| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VGI0071 | pCo hygro | Invitrogen | 果蝇细胞表达系统 |
Comments for pCoHygro
4526 nucleotides
copia promoter: bases 500-746
Hygromycin resistance gene: bases 781-1830
SV40 early polyadenylation sequence: bases 2146-2280
pUC origin: bases 2648-3321 （complementary strand）
Ampicillin （bla） resistance gene: bases 3466-4326 （complementary strand）
bla promoter: bases 4327-4425 （complementary strand）
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VRA0340 | pSilencer 3.1-H1 hygro | Ambion | RNAi载体 |
The pSilencer vectors employ RNA polymerase III （pol III） promoters which generate large amounts of small RNA using relatively simple promoter and terminator sequences. They also include an antibiotic resistance gene that provides a mechanism to select for transfected cells that express the introduced DNA.
This Ambion® vector is for the expression of siRNA. It has an antibiotic resistance gene （hygromycin） to enable selection of transfected cells and features the human RNA polymerase III promoter （U6）. Sufficient reagents are provided for 20 reactions.
• Select transfected cells to enrich the population of cells expressing your siRNA
• Eliminate the need to synthesize RNA oligonucleotides for RNAi experiments
• Supplied linearized and ready for ligation
Compensate for Low Transfection Efficiencies and Perform Long-Term Studies
The use of mammalian siRNA expression vectors with antibiotic selectable markers conveys many benefits. Selectable markers can help compensate for poor plasmid transfection efficiencies seen with some cell lines. In these cases, only a fraction of the transfected cells express the siRNA, and reduction in target gene expression with even a potent siRNA can be difficult to detect. Use of a selectable marker and transient antibiotic selection permits.