CRISPR Human Genome 80K Knockout Libraries现货!


CRISPR Human Genome 80K Knockout Libraries现货!

Cellecta是一家生命科学研究公司,成立于2006年,总部位于美国加利福尼亚州的山景城。该公司专注于开发和提供高质量的基因组学和功能基因组学研究工具,帮助科学家研究基因在生物学和疾病中的作用。

Cellecta的主要产品包括基因编辑工具、RNAi筛查工具、CRISPR筛查工具、基因表达分析工具等。这些工具可以用于基于细胞的研究、动物模型研究和临床前药物筛选等领域。除了产品外,Cellecta还提供定制化研究服务,包括基因编辑、CRISPR筛查、RNAi筛查、基因表达分析等服务。该公司的客户包括学术机构、生物技术公司和制药公司等。

 

简介:

Cellecta的第一个单模块人类全基因组CRISPR敲除文库设计得足够小(约80000个构建体),可以用大多数标准的人类细胞模块进行方便的全基因组“缺失活力”筛选。针对每个基因有4sgRNA,所有这些都是使用Doench等人描述的有效指南标准设计的,并且包含Cellecta设计的HEAT sgRNA-tracrRNA结构,我们已经证明它比标准sgRNA设计产生了更有力的筛选结果,该文库具有足够深的基因覆盖范围,可以产生有效的结果。

 

特点:

单个文库,约80000个构建体,靶向约19000个蛋白质编码基因

每个基因都被基于Doench等标准设计的4sgRNA靶向

所有人类蛋白质编码基因覆盖19000多个基因靶点

此库可以使用RFPPuro、仅PuroRFPNeo标记获得。

 

产品信息:

货号

品名

规格

品牌

KOHGW-80K-P

CRISPR Human Genome 80K Knockout Library

200ug

cellecta

 

上海金畔生物大量现货,欢迎来询!

 

人类凝血因子(Human Factor IXa β)HFIXa 1080说明书

 Enzyme Research 是一家拥有超过25年的酶研究的实验室,生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质,赢得了世界各地凝血研究实验室的信任。

Human Factor IXa β

 

HFIXa 1080             Human Factor IXa β

Prepared from Human Factor IX by activation with Bovine Factor XIa. This Bovine Factor XIa is removed after activation. Complete activation is observed by SDS-PAGE. The Factor XIa activates Factor IX in a two-step reaction. In the first step, an internal Arg-Ala bond is cleaved, and in the second step, an Arg-Val bond is cleaved. The second cleavage leads to the liberation of an activation peptide from the NH2-terminal portion of the heavy chain to produce Factor IXaβ.

Buffer composition = 20 mM Tris-HCl/0.1 M NaCl /pH 7.4
*Extinction Coefficient (1%) = 14.3 
One unit =5 µg
Molecular Weight = 45,000 daltons

 

重组人CB1蛋白VLP Human CB1 Protein-VLP

重组人CB1蛋白VLP Human CB1 Protein-VLP

产品说明书

FAQ

COA

已发表文献

产品信息

 

产品名称

产品编号

规格

Recombinant Human CB1 Protein-VLP

95035ES60

100 μg

95035ES80

1 mg

 

性能参数

 

蛋白别名(Synonyms)

CB1 / CNR1 / CNR

表达系统(Source)

HEK293 Cells

表达区间(AA sequence)

Accession #P21554: Met1 – Leu472

内毒素(Endotoxin)

< 1EU per μg by the LAL method.

纯度(Purity)

>90% as determined by DLS.

制剂(Formulation)

115mM Gly 4% trehalose

活性(Activity)

ELISAImmobilized Human CB1 VLP at 5 ug/mL (30 uL/well) can bind Monoclonal Anti-CB1 / CNR1 Reference Antibody (nimacimab), The EC50 was approximately 0.04092 µg/ml.

应用(Applications

ELISA, BLI, SPR, Immunization

稀释(dilution)

Dilute with PBS.

 

储存条件

 

The product should be stored at –85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

 

  1. Please operate with lab coats and disposable gloves,for your safety.       
  2. This product is for research use only.        

   Ver. CN20240115

重组人CB1蛋白VLP Human CB1 Protein-VLP

暂无内容

重组人CB1蛋白VLP Human CB1 Protein-VLP

暂无内容

产品信息

 

产品名称

产品编号

规格

Recombinant Human CB1 Protein-VLP

95035ES60

100 μg

95035ES80

1 mg

 

性能参数

 

蛋白别名(Synonyms)

CB1 / CNR1 / CNR

表达系统(Source)

HEK293 Cells

表达区间(AA sequence)

Accession #P21554: Met1 – Leu472

内毒素(Endotoxin)

< 1EU per μg by the LAL method.

纯度(Purity)

>90% as determined by DLS.

制剂(Formulation)

115mM Gly 4% trehalose

活性(Activity)

ELISAImmobilized Human CB1 VLP at 5 ug/mL (30 uL/well) can bind Monoclonal Anti-CB1 / CNR1 Reference Antibody (nimacimab), The EC50 was approximately 0.04092 µg/ml.

应用(Applications

ELISA, BLI, SPR, Immunization

稀释(dilution)

Dilute with PBS.

 

储存条件

 

The product should be stored at –85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

 

  1. Please operate with lab coats and disposable gloves,for your safety.       
  2. This product is for research use only.        

   Ver. CN20240115

重组人CB1蛋白VLP Human CB1 Protein-VLP

暂无内容

重组人CB1蛋白VLP Human CB1 Protein-VLP

暂无内容

重组人GIPR蛋白VLP Human GIPR Protein-VLP

重组人GIPR蛋白VLP Human GIPR Protein-VLP

产品说明书

FAQ

COA

已发表文献

产品信息

 

产品名称

产品编号

规格

Recombinant Human GIPR Protein-VLP

95023ES60

100 μg

95023ES80

1 mg

 

性能参数

 

蛋白别名(Synonyms)

Growth hormone secretagogue receptor type 1; GHS-R; GH-releasing peptide receptor; Ghrelin receptor

表达系统(Source)

HEK293 Cells

表达区间(AA sequence)

Accession #P48546: Met1 – Cys466

内毒素(Endotoxin)

< 1EU per μg by the LAL method.

纯度(Purity)

> 85% as determined by DLS.

制剂(Formulation)

115mM Gly 4% trehalose

应用(Applications

ELISA, Kinetics (BLI), Kinetics (SPR), Immunization

稀释(dilution)

Dilute with PBS.

 

储存条件

 

The product should be stored at –85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

 

  1. Please operate with lab coats and disposable gloves,for your safety.       
  2. This product is for research use only.  

 

Ver. CN20240115     

重组人GIPR蛋白VLP Human GIPR Protein-VLP

暂无内容

重组人GIPR蛋白VLP Human GIPR Protein-VLP

暂无内容

产品信息

 

产品名称

产品编号

规格

Recombinant Human GIPR Protein-VLP

95023ES60

100 μg

95023ES80

1 mg

 

性能参数

 

蛋白别名(Synonyms)

Growth hormone secretagogue receptor type 1; GHS-R; GH-releasing peptide receptor; Ghrelin receptor

表达系统(Source)

HEK293 Cells

表达区间(AA sequence)

Accession #P48546: Met1 – Cys466

内毒素(Endotoxin)

< 1EU per μg by the LAL method.

纯度(Purity)

> 85% as determined by DLS.

制剂(Formulation)

115mM Gly 4% trehalose

应用(Applications

ELISA, Kinetics (BLI), Kinetics (SPR), Immunization

稀释(dilution)

Dilute with PBS.

 

储存条件

 

The product should be stored at –85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

 

  1. Please operate with lab coats and disposable gloves,for your safety.       
  2. This product is for research use only.  

 

Ver. CN20240115     

重组人GIPR蛋白VLP Human GIPR Protein-VLP

暂无内容

重组人GIPR蛋白VLP Human GIPR Protein-VLP

暂无内容

Human IL-21 重组人白细胞介素-21(人白介素-21)

Human IL-21 重组人白细胞介素-21(人白介素-21)

产品说明书

FAQ

COA

已发表文献

Hunan IL-21 protein is a molecule that is found in the immune system of individuals. It is a type of interleukin, which is a protein that helps in the communication between immune cells. IL-21 plays a crucial role in regulating the body's immune response, specifically in the development, differentiation, and function of certain immune cells, such as T cells and natural killer cells.

 

性能参数

Synonyms

Interleukin-21, IL-21, Za11

Uniprot No.

Q9HBE4

Source

HEK293 cells-derived human IL-21 protein, Gln30-Ser162, with N-terminal Flag tag.

Molecular Weight

The protein has a predicted MW of 16.5 kDa. And it migrates as 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

< 0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS.

Reconstitution

Centrifuge tubes before opening. Dissolve lyophilized protein with PBS. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

储存条件

The product should be stored at -25~15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~15℃ under sterile conditions after reconstitution.

 

注意事项

1.Please operate with lab coats and disposable gloves, for your safety.

2.This product is for research use only.

 

产品数据

Human IL-21 重组人白细胞介素-21(人白介素-21)                                

Ver. CN20230705

 

Human IL-21 重组人白细胞介素-21(人白介素-21)

暂无内容

Human IL-21 重组人白细胞介素-21(人白介素-21)

暂无内容

Hunan IL-21 protein is a molecule that is found in the immune system of individuals. It is a type of interleukin, which is a protein that helps in the communication between immune cells. IL-21 plays a crucial role in regulating the body's immune response, specifically in the development, differentiation, and function of certain immune cells, such as T cells and natural killer cells.

 

性能参数

Synonyms

Interleukin-21, IL-21, Za11

Uniprot No.

Q9HBE4

Source

HEK293 cells-derived human IL-21 protein, Gln30-Ser162, with N-terminal Flag tag.

Molecular Weight

The protein has a predicted MW of 16.5 kDa. And it migrates as 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

< 0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS.

Reconstitution

Centrifuge tubes before opening. Dissolve lyophilized protein with PBS. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

储存条件

The product should be stored at -25~15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~15℃ under sterile conditions after reconstitution.

 

注意事项

1.Please operate with lab coats and disposable gloves, for your safety.

2.This product is for research use only.

 

产品数据

Human IL-21 重组人白细胞介素-21(人白介素-21)                                

Ver. CN20230705

 

Human IL-21 重组人白细胞介素-21(人白介素-21)

暂无内容

Human IL-21 重组人白细胞介素-21(人白介素-21)

暂无内容

人类凝血因子(Human Factor VIIa) HFVIIa说明书

 Enzyme Research 是一家拥有超过25年的酶研究的实验室,生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质,赢得了世界各地凝血研究实验室的信任。

Human Factor VIIa

 

HFVIIa             Human Factor VIIa

Prepared from purified Human Factor VII using Human Factor XIIa. The Factor Xlla is removed using affinity chromatography. Purity is determined by SDS-PAGE. Human Factor VIIa reduces to 29,500 and 23,500 with the addition of 2-mercaptoethanol. Activity is determined via clotting assay.  Factor Vlla, in the presence of calcium ions and Tissue factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa.

Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/pH 7.4
*Extinction Coefficient (1%) = 13.9
Molecular Weight = 50,000 daltons

Also available: des-Gla-Human Factor VIIa and 
Factor VIIa inactivated

Nestin anti-Human Antibody说明书

ESI BIO的使命是成为客户信赖的合作伙伴,将科学发现转化为诊所。我们通过提供突破性的干细胞技术作为研究级和治疗级产品来实现这一目标,从研究到诊所的照明,以帮助我们的客户实现他们治疗疾病的目标。

ESI BIO建立在丰富的创新和科学的基础之上。我们于2000年在新加坡成立了ES Cell International,在那里我们通过制造个可翻译的临床级胚胎干细胞系来创建历史,以便分发给研究界。

 

ESI BIO现在开展业务Alameda CA. 我们是BioTime,Inc。拥有的以干细胞为基础的子公司的骄傲家族的成员,我们从中受益于的科学,知识产权和干细胞技术的深层来源。

抗体

 

胚胎干细胞(ESCs)和诱导多能干细胞(iPSCs)的表征是干细胞研究中的关键步骤。验证起始细胞群的常规过程应该简单,快速,重要的是可靠,以确保准确和一致的结果。ESI BIO为您提供关键抗体形式和活体染色工具,这些工具专为干细胞直接开发和测试而开发。

快速而可靠的多能性验证
ESI BIO的BioLite™抗体可直接染色培养液中的活细胞,因此无需牺牲可用于下游实验的有价值物质。

· 确认多能性标志物表达

· 允许在染色后继续细胞培养

· 低内毒素,不含Sodium azide的配方

· 无需固定或透化,仅需孵育30分钟

 

图1. [A]小鼠ES细胞的IF分析。用BioLite SSEA-1(Dy-Light 488)抗体染色后培养细胞和相同视野的相差图。[B] IF重组成人成纤维细胞第11天的原代iPS细胞集落的IF分析。用BioLite TRA-1-81(DyLight 488)抗体染色后培养细胞和相同视野的相差图。以10X放大率拍摄图像。

Nestin抗人类抗体

Nestin anti-Human Antibody, 100 μL

巢蛋白是在干细胞中表达的VI类中间丝蛋白。

SKU: ST11023

巢蛋白是在发育中枢神经系统(CNS)的干细胞中表达但在成熟CNS细胞中不表达的VI类中间丝蛋白。其表达广泛用作源自人ES和iPS细胞的神经谱系细胞的主要标记。巢蛋白也在非神经干细胞群中表达,例如胰岛祖细胞和造血祖细胞。

浓度 0.5毫克/毫升

物种反应性 人的

主办 小鼠单克隆

克隆 10C2

同型 的IgG1

免疫原 来自人巢蛋白的重组150个氨基酸片段与谷胱甘肽S转移酶缀合。

公式 含水缓冲液,0.09%Sodium azide,(pH7.2)。

储存和稳定性 储存在2-8°C。根据指示存放,自收到之日起6个月内稳定。

应用程序测试 免疫细胞化学/免疫荧光(ICC / IF)

推荐的稀释液 免疫细胞化学/免疫荧光1:100

Western Blot 1:100

建议针对每种应用滴定抗体以获得*性能。

参考 Lendahl,U。,et al。(1990)CNS干细胞表达一类新的中间丝蛋白。Cell 60(4):585-595。PMID:1689217。

 

Carpenter,MK,et al。(2001)富集人胚胎干细胞的神经元和神经前体。Exp Neurol 172(2):383-397。PMID:11716562。

 

Gilyarov,AV(2008)Nestin in central nervous system cells。Neurosci Behav Physiol 38(2):165-169。PMID:18197384。

 

 

货号

品名

规格

品牌

ST11006

BioLite™ SSEA-1 (DyLight 488) anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

ST11008

BioLite™ TRA-1-81 (DyLight 488) anti-Human Antibody, 100 μL

 100 μL

esibio

ST11023

Nestin anti-Human Antibody, 100 μL

 100 μL

esibio

ST11003

Oct4 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

 ST11001

Sox2 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

ST11013

SSEA-1 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

 ST11014

SSEA-3 anti-Human/Mouse Antibody, 100 µL

 100 µL

esibio

ST11015

SSEA-4 anti-Human Antibody, 100 µL

 100 µL

esibio

ST11018

TRA-1-60 (PE) anti-Human Antibody, 100 μL

 100 μL

esibio

ST11016

TRA-1-60 anti-Human Antibody, 100 µL

 100 µL

esibio

ST11017

TRA-1-81 anti-Human Antibody, 100 µL

 100 µL

esibio

 ES-700

ESI-017 Human Embryonic Stem Cell Line

ea

esibio

ES-701

ESI-035 Human Embryonic Stem Cell Line

ea

esibio

 ES-702

ESI-049 Human Embryonic Stem Cell Line

ea

esibio

ES-703

ESI-051 Human Embryonic Stem Cell Line

ea

esibio

ES-704

ESI-053 Human Embryonic Stem Cell Line

ea

esibio

ES-706

Human Embryonic Stem Cell Bundle

ea

esibio

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多个品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发,欢迎合作。

 

重组人FSHα&β蛋白 Recombinant Human FSH alpha&beta Protein,hFc Tag

重组人FSHα&β蛋白 Recombinant Human FSH alpha&beta Protein,hFc Tag

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

FSH alpha & FSH beta;FSH alphaβFSH alpha/beta

表达区间及表达系统(Source)

Human FSH alpha&beta Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala25-Ser116 (FSH alpha) & Asn19-Glu129 (FSH beta).[Accession | P01215(FSH alpha)&P01225 (FSH beta)]

分子量大小(Molecular Weight)

The protein has a predicted MW of 10.20 kDa (FSH alpha) and 39.2 kDa (FSH beta). Due to glycosylation, the protein migrates to 25-30 kDa (FSH alpha) and 50-60 kDa (FSH beta) based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名(Synonyms)

FSH alpha & FSH beta;FSH alphaβFSH alpha/beta

表达区间及表达系统(Source)

Human FSH alpha&beta Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala25-Ser116 (FSH alpha) & Asn19-Glu129 (FSH beta).[Accession | P01215(FSH alpha)&P01225 (FSH beta)]

分子量大小(Molecular Weight)

The protein has a predicted MW of 10.20 kDa (FSH alpha) and 39.2 kDa (FSH beta). Due to glycosylation, the protein migrates to 25-30 kDa (FSH alpha) and 50-60 kDa (FSH beta) based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

人极低密度脂蛋白 Human VLDL|Human Very Low Density Lipoprotein(Human VLDL)

人极低密度脂蛋白 Human VLDL|Human Very Low Density Lipoprotein(Human VLDL)

产品说明书

FAQ

COA

已发表文献

产品描述

极低密度脂蛋白(VLDL)为一种由肝脏制造的脂蛋白,在血液中转换成低密度脂蛋白,其主要功能是传送内源性三酸甘油脂、胆固醇、磷脂和胆固醇脂。为五种主要的脂蛋白按照分子量大小,主要分为以下几种(从高到低):乳糜(CM)、极低密度脂蛋白 (VLDL)、中间密度脂蛋白 (IDL)、低密度脂蛋白 (LDL)、高密度脂蛋白(HDLVLDL可以使脂肪和胆固醇在水溶性的血液中移动。VLDL粒子直径为30~80 nm
人源极低密度脂蛋白Human Very Low Density LipoproteinHuman VLDL),d≤1.006 g/mL来自健康人源血浆,超速离心纯化获得,通过琼脂糖电泳检测其均匀性和纯度本产品为无菌充氮包装,可以直接稀释使用。

 

产品性质

纯度Purity by Agarose Gel

98%

浓度 (Concentration)

0.8-2.0 mg/ml

外观Appearance

乳状液体

缓冲液组分(Buffer

1 µM EDTA in PBS, pH 7.4

 

稀释方法

根据实验需要用PBS磷酸盐缓冲液或细胞培养液稀释即可。

 

运输与保存方法

冰袋运输4ºC无菌,建议避光,收到货后稳定保存6周。切忌冻存!

 

注意事项
1) 本品的稀释工作液极不稳定,建议即配即用;
2) 长期贮存可能会有沉淀析出,属于正常现象,低速离心2 min去除沉淀即可使用;
3) 为了您的安全和健康,请穿实验服并戴一次性手套操作。

 

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Q:如何终止该产品反应?

A:加入过量 EDTA。

Q:该产品需要用什么缓冲液溶解?

A:根据实验需要用 PBS 磷酸盐缓冲液或细胞培养液稀释即可

Q:该产品如何储存?

A4ºC 无菌避光,收到货后可稳定保存 6 周。但不可冻存。

Q:该产品长期保存会出现少量沉淀,还可以使用吗?

A:这是正常现象。低速离心 1 ~2min 去除沉淀物,即得到澄清液

人极低密度脂蛋白 Human VLDL|Human Very Low Density Lipoprotein(Human VLDL)

暂无内容

产品描述

极低密度脂蛋白(VLDL)为一种由肝脏制造的脂蛋白,在血液中转换成低密度脂蛋白,其主要功能是传送内源性三酸甘油脂、胆固醇、磷脂和胆固醇脂。为五种主要的脂蛋白按照分子量大小,主要分为以下几种(从高到低):乳糜(CM)、极低密度脂蛋白 (VLDL)、中间密度脂蛋白 (IDL)、低密度脂蛋白 (LDL)、高密度脂蛋白(HDLVLDL可以使脂肪和胆固醇在水溶性的血液中移动。VLDL粒子直径为30~80 nm
人源极低密度脂蛋白Human Very Low Density LipoproteinHuman VLDL),d≤1.006 g/mL来自健康人源血浆,超速离心纯化获得,通过琼脂糖电泳检测其均匀性和纯度本产品为无菌充氮包装,可以直接稀释使用。

 

产品性质

纯度Purity by Agarose Gel

98%

浓度 (Concentration)

0.8-2.0 mg/ml

外观Appearance

乳状液体

缓冲液组分(Buffer

1 µM EDTA in PBS, pH 7.4

 

稀释方法

根据实验需要用PBS磷酸盐缓冲液或细胞培养液稀释即可。

 

运输与保存方法

冰袋运输4ºC无菌,建议避光,收到货后稳定保存6周。切忌冻存!

 

注意事项
1) 本品的稀释工作液极不稳定,建议即配即用;
2) 长期贮存可能会有沉淀析出,属于正常现象,低速离心2 min去除沉淀即可使用;
3) 为了您的安全和健康,请穿实验服并戴一次性手套操作。

 

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20606JP75

Human DiI-Ac-LDL 红色荧光标记人源乙酰化低密度脂蛋白

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Human High Ox-LDL 人源高氧化程度低密度脂蛋白

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Human HDL 人源高密度脂蛋白

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Human LDL 人源低密度脂蛋白

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Human DiI-LDL红色荧光标记人源低密度脂蛋白

300μg

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Q:如何终止该产品反应?

A:加入过量 EDTA。

Q:该产品需要用什么缓冲液溶解?

A:根据实验需要用 PBS 磷酸盐缓冲液或细胞培养液稀释即可

Q:该产品如何储存?

A4ºC 无菌避光,收到货后可稳定保存 6 周。但不可冻存。

Q:该产品长期保存会出现少量沉淀,还可以使用吗?

A:这是正常现象。低速离心 1 ~2min 去除沉淀物,即得到澄清液

人极低密度脂蛋白 Human VLDL|Human Very Low Density Lipoprotein(Human VLDL)

暂无内容

重组人CD20蛋白VLP Human CD20 Protein-VLP

重组人CD20蛋白VLP Human CD20 Protein-VLP

产品说明书

FAQ

COA

已发表文献

产品信息

 

产品名称

产品编号

规格

Recombinant Human CD20 Protein-VLP

95038ES60

100 μg

95038ES80

1 mg

 

性能参数

 

蛋白别名(Synonyms)

MS4A1,CD20,MS4A-1

表达系统(Source)

HEK293 Cells

表达区间(AA sequence)

Accession #P11836: Met1 – Pro297

内毒素(Endotoxin)

< 1EU per μg by the LAL method.

纯度(Purity)

>90% as determined by DLS.

制剂(Formulation)

115mM Gly 4% trehalose

活性(Activity)

ELISA Data 1Immobilized Human CD20 VLP at 5 ug/mL (30 ul/well) can bind obinutuzumab(P214063), The EC50 was approximately 0.006752 ug/ml.

ELISA Data 2Immobilized Human CD20 VLP at 5 ug/mL (30 ul/well) can bind Anti-CD20 / MS4A1 Reference Antibody (divozilimab), The EC50 was approximately 0.01698 ug/ml.

应用(Applications

ELISA, BLI, SPR, Immunization

稀释(dilution)

Dilute with PBS.

 

储存条件

 

The product should be stored at –85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

 

  1. Please operate with lab coats and disposable gloves,for your safety.       
  2. This product is for research use only.      

   Ver. CN20240115

重组人CD20蛋白VLP Human CD20 Protein-VLP

暂无内容

重组人CD20蛋白VLP Human CD20 Protein-VLP

暂无内容

产品信息

 

产品名称

产品编号

规格

Recombinant Human CD20 Protein-VLP

95038ES60

100 μg

95038ES80

1 mg

 

性能参数

 

蛋白别名(Synonyms)

MS4A1,CD20,MS4A-1

表达系统(Source)

HEK293 Cells

表达区间(AA sequence)

Accession #P11836: Met1 – Pro297

内毒素(Endotoxin)

< 1EU per μg by the LAL method.

纯度(Purity)

>90% as determined by DLS.

制剂(Formulation)

115mM Gly 4% trehalose

活性(Activity)

ELISA Data 1Immobilized Human CD20 VLP at 5 ug/mL (30 ul/well) can bind obinutuzumab(P214063), The EC50 was approximately 0.006752 ug/ml.

ELISA Data 2Immobilized Human CD20 VLP at 5 ug/mL (30 ul/well) can bind Anti-CD20 / MS4A1 Reference Antibody (divozilimab), The EC50 was approximately 0.01698 ug/ml.

应用(Applications

ELISA, BLI, SPR, Immunization

稀释(dilution)

Dilute with PBS.

 

储存条件

 

The product should be stored at –85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

 

  1. Please operate with lab coats and disposable gloves,for your safety.       
  2. This product is for research use only.      

   Ver. CN20240115

重组人CD20蛋白VLP Human CD20 Protein-VLP

暂无内容

重组人CD20蛋白VLP Human CD20 Protein-VLP

暂无内容