GSH和GSSG检测试剂盒(S0053)

GSH和GSSG检测试剂盒

产品编号: S0053

产品包装:共100次
选择包装
共100次

说明书下载

586.00 10
价格: ¥ 586.00

产品简介
使用说明
产品图片
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
S0053 GSH和GSSG检测试剂盒 共100次 586.00元

GSH和GSSG检测试剂盒(GSH and GSSG Assay Kit)是一种简单易行的可以分别检测出GSH(还原型谷胱甘肽)和GSSG(氧化型谷胱甘肽,oxidized glutathione disulfide)含量的检测试剂盒。
谷胱甘肽(glutathione)是一种由3个氨基酸残基组成的小肽,全称为谷氨酰-半胱氨酰-甘氨酸,英文名称为glutamyl-cysteinyl-glycine,简称为glutathione。由于半胱氨酸上的巯基(SH)为谷胱甘肽的活性基团,所以常简写为G-SH或GSH。谷胱甘肽包括还原型谷胱甘肽(reduced glutathione, 常称为GSH)和氧化型谷胱甘肽(oxidized glutathione disulfide)两种形式。由于氧化型谷胱甘肽是由两个GSH通过巯基脱氢而成,所以常简写为G-S-S-G或GSSG。还原型谷胱甘肽是绝大多数活细胞中巯基的主要来源,对于维持蛋白质中巯基适当的氧化还原状态有重要作用,并且是动物细胞中关键的抗氧化剂。总谷胱甘肽中通常90-95%为还原型谷胱甘肽。
通过谷胱甘肽还原酶把GSSG还原成GSH,而GSH可以和生色底物DTNB反应产生黄色的TNB和GSSG。适当配制反应体系,前后两个反应合并起来后,总谷胱甘肽(GSSG+GSH)就相当于一个颜色产生的限速因素,总谷胱甘肽的量就决定了黄色的TNB形成量。从而通过测定A412就可以计算出总谷胱甘肽的量。用适当试剂先清除样品中的GSH,然后利用上述反应原理就可以测定出GSSG的含量。用总谷胱甘肽(GSSG+GSH)的量扣除GSSG的含量,就可以计算出GSH的含量。
本试剂盒的具体反应原理如下:

GSH和GSSG检测试剂盒(S0053)
GSH和GSSG检测试剂盒(S0053)

两个反应相合并:

GSH和GSSG检测试剂盒(S0053)

本试剂盒可以检测动物组织、血浆、红细胞、和培养细胞或其它适当样品中GSH和GSSG的含量。
本试剂盒提供了蛋白去除试剂M,可以更加准确地测定出含有蛋白的样品中的GSH和GSSG的量。
本试剂盒的检测下限为0.5μM。一个试剂盒共可以进行100次检测,可以测定100个样品的总谷胱甘肽或GSSG的含量,或可以测定出50个样品中GSH和GSSG的各自含量。
包装清单:

产品编号 产品名称 包装
S0053-1 总谷胱甘肽检测缓冲液 60ml
S0053-2 谷胱甘肽还原酶 150μl
S0053-3 氧化型谷胱甘肽 (GSSG) 5mg
S0053-4 DTNB 4.5mg
S0053-5 蛋白去除试剂M 1g
S0053-6 NADPH 4mg
S0053-7 DMSO 1.5ml
S0053-8 GSH清除辅助液 2ml
S0053-9 GSH清除试剂 500μl
说明书 1份

保存条件:
-20℃保存,一年有效。GSSG配制成溶液后,需适当分装,-20℃保存至少3个月有效。DTNB溶解于DMSO后,需适当分装,-20℃保存至少3个月有效。蛋白去除试剂M配制成溶液后仅限当天使用。NADPH溶解后,适当分装,-70℃保存。稀释的GSH清除辅助液和GSH清除试剂溶液均须新鲜配制使用。
注意事项:
本试剂盒检测时牵涉到氧化还原反应,所有氧化剂或还原剂都会干扰本试剂盒的测定。特别是DTT、巯基乙醇等含有巯基的试剂会严重干扰本试剂盒的测定,请尽量避免。
一定要严格控制反应时的温度和反应时间,否则每次都需做标准曲线。
NADPH等试剂不太稳定,要严格按照后续说明操作,谨防失活。
蛋白去除试剂M溶液必须新鲜配制并限当日使用。GSH清除试剂也须新鲜稀释后使用。
蛋白去除试剂M较难溶解,可以通过剧烈vortex并适当加热(不超过37℃)以促进溶解。
DMSO在4℃、冰浴等较低温度情况下会凝固,可以20-25℃水浴温育片刻至全部融解后使用。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1.试剂盒的准备工作:
a.GSSG储备液的配制:在本试剂盒提供的5mg GSSG中加入816微升Milli-Q级纯水,溶解并混匀,即为GSSG储备液,浓度为10mM。除立即待用部分外,其余GSSG储备液适当分装后-20℃保存。
b.DTNB储备液的配制:在本试剂盒提供的4.5mg DTNB中加入1.5毫升本试剂盒提供的DMSO,溶解并混匀,即为DTNB储备液。除立即待用部分外,其余DTNB储备液适当分装后-20℃保存。
c.蛋白去除试剂M溶液的配制:称取0.2克蛋白去除试剂M,加入4毫升总谷胱甘肽检测缓冲液,配制成4毫升5%的水溶液。蛋白去除试剂M溶液必须新鲜配制并限当天使用。
d.NADPH储备液(40mg/ml)的配制:在本试剂盒提供的4mg NADPH中加入100微升Milli-Q级纯水,溶解并混匀,即为NADPH储备液。除立即待用部分外,其余NADPH储备液适当分装后-70℃保存。
e.5倍稀释谷胱甘肽还原酶的配制:取50微升谷胱甘肽还原酶,加入200微升总谷胱甘肽检测缓冲液,混匀,即成5倍稀释的谷胱甘肽还原酶。
f.总谷胱甘肽检测工作液的配制:根据待检测的样品数参考下表配制适当量的总谷胱甘肽检测工作液,表中三种试剂按比例混合后即为总谷胱甘肽检测工作液。

1个样品 10个样品 20个样品
5倍稀释谷胱甘肽还原酶 6.6μl 66μl 132μl
DTNB储备液 6.6μl 66μl 132μl
总谷胱甘肽检测缓冲液 150μl 1.5ml 3ml

g.  0.5mg/ml NADPH的配制:取10微升NADPH储备液,加入790微升总谷胱甘肽检测缓冲液,混匀即为0.5mg/ml NADPH。每检测一个样品需50微升0.5mg/ml NADPH。
h.  稀释的GSH清除辅助液的配制:在47微升Milli-Q级纯水中加入53微升GSH清除辅助液,立即混匀。稀释后的GSH清除辅助液不太稳定,每次使用时均须新鲜配制,并仅限当日使用。
i.  GSH清除试剂工作液的配制:10.8微升GSH清除试剂中加入89.2微升无水乙醇,立即混匀。GSH清除剂工作液每次也须新鲜配制。
2.标准品的准备:
a.  把10mM GSSG储备液用蛋白去除试剂M溶液稀释成15μM GSSG溶液。然后依次稀释成10、5、2、1、0.5μM GSSG溶液。取15、10、5、2、1、0.5μM GSSG溶液六个点做标准曲线。注意:由于GSSG在蛋白去除试剂M溶液中不太稳定,用蛋白去除试剂M溶液配制的GSSG溶液必须新鲜配制后使用,不可冻存后再使用。
b.  如果需要测定样品中GSSG含量,按照每100微升标准品加入20微升稀释的GSH清除辅助液的比例加入稀释的GSH清除辅助液,立即vortex混匀。然后按照每100微升标准品加入4微升GSH清除试剂工作液的比例加入GSH清除试剂工作液,立即vortex混匀,25℃反应60分钟。即可用于后续的GSSG含量的检测。
3.待测总谷胱甘肽含量样品和标准品的准备:
a.组织样品的准备。取组织用液氮速冻,然后研成粉末。每10毫克研碎的组织粉末,加入30微升蛋白去除试剂M溶液,充分Vortex。再加入70微升蛋白去除试剂M溶液,用玻璃匀浆器充分匀浆(对于比较容易匀浆的组织可以不用液氮速冻等处理,而直接加入适量蛋白去除试剂M溶液进行匀浆)。4℃放置10分钟后,10,000g 4℃离心10分钟,取上清用于总谷胱甘肽的测定。样品需暂时4℃保存,不立即测定的样品可以-70℃保存,但不宜超过10天。对于处理好的组织样品通常需用蛋白去除试剂M溶液进行适当稀释后再进行测定,稀释倍数通常为5-20倍。
b.细胞样品的准备。请尽量使用新鲜的细胞进行测定,而不要使用冻存的细胞进行测定。PBS洗涤细胞一次,离心收集细胞,吸尽上清。加入细胞沉淀体积3倍量的蛋白去除试剂M溶液,即如果细胞沉淀为10微升,则加入30微升蛋白去除试剂M溶液,充分Vortex。(细胞沉淀的体积可以根据细胞沉淀的重量进行估算。收集细胞前后分别对离心管进行称重,从而就可以计算出细胞沉淀的重量。10毫克细胞沉淀的体积可以粗略地看做10微升。) 然后利用液氮和37℃水浴对样品进行两次快速的冻融。4℃或冰浴放置5分钟。4℃,10,000g离心10分钟。取上清用于总谷胱甘肽的测定。样品需暂时4℃保存,不立即测定的样品可以-70℃保存,但不宜超过10天。对于处理好的细胞样品通常需用蛋白去除试剂M溶液进行适当稀释后再进行测定,稀释倍数可以高达20倍。
c.红细胞或血浆样品的准备。请尽量使用新鲜的血液进行测定。600g离心10分钟,沉淀为红细胞,上清为血浆。对于红细胞,用PBS洗涤两次。取约50微升红细胞沉淀或血浆,加入50微升蛋白去除试剂M溶液,充分Vortex。4℃或冰浴放置10分钟。4℃,10,000g离心10分钟。取上清用于总谷胱甘肽的测定。样品需暂时4℃保存,不立即测定的样品可以-70℃保存,但不宜超过10天。对于处理好的红细胞样品最后需用蛋白去除试剂M溶液稀释10倍后再进行后续的测定,而对于血浆样品,应直接取10微升进行测定。
d.说明:对于一些谷胱甘肽含量特别低的样品,可以通过冷冻干燥进行浓缩后再进行测定。
4. 待测GSSG含量样品的准备:
取部分上述准备好的待测总谷胱甘肽含量的样品,按照每100微升样品加入20微升稀释的GSH清除辅助液的比例加入稀释的GSH清除辅助液,立即vortex混匀。再按照每100微升样品加入4微升GSH清除试剂工作液的比例加入GSH清除工作液,立即vortex混匀,25℃反应60分钟。通过上述反应可以清除高达50μM的GSH,如果样品中GSH含量过高需进行适当稀释后再进行去除GSH的操作。通过上述处理就可以用于后续的测定。
5. 样品和标准品的测定:
a.  参考下表,使用96孔板,依次加入样品或标准品,混匀。加入150微升总谷胱甘肽检测工作液后,混匀,25℃或室温孵育5分钟。

空白对照 (blank) 标准曲线 (standard) 样品(sample)
样品或标准品 0 μl 10 μl x μl
蛋白去除试剂M溶液 10 μl 0 μl 10-x μl
总谷胱甘肽检测工作液 150 μl 150 μl 150 μl
25℃或室温孵育 5 min 5 min 5 min
0.5mg/ml NADPH 50 μl 50 μl 50 μl

b.加入50微升0.5mg/ml NADPH溶液,混匀。
c.立即用酶标仪测定A412,每5分钟测定一次或实时测定,共测定25分钟,测得5个数据。(说明:为了简化实验步骤,可以在加入NADPH溶液混匀后25分钟,仅测定一次A412)。如果仪器可以设置温度,把温度设置在25℃,否则就在室温状况下测定。如果酶标仪不能测定A412,可以测定405-414nm附近范围的吸光度。如果标准曲线良好,但样品的吸光度比较低,可以延长孵育时间至30-60分钟,标准品和样品的吸光度在一定范围内会随时间的延长接近于线性增加的。
注意:如果进行GSSG含量测定,标准品也须平行地进行去除GSH的相关操作,以减小误差。如果样品需同时测定总谷胱甘肽含量和GSSG含量,由于两者的检测体系不同,须分别单独做标准曲线。
d.标准品的实测效果参考图1。
GSH和GSSG检测试剂盒(S0053) 图1. GSSG标准曲线实测效果图。图中数据仅供参考,实际的检测效果可能会因具体反应条件的不同而有所不同。 6.样品中总谷胱甘肽含量的计算:
a.单点测定法:即反应25分钟(或30-60分钟)后仅测定一次吸光度。根据不同浓度标准品测得的不同吸光度作出标准曲线。样品对照标准曲线即可计算出总谷胱甘肽(标准曲线计算得到的GSSG浓度乘以2)或GSSG的含量。实际计算出来的总谷胱甘肽的含量相当于把氧化型谷胱甘肽的含量乘以2再加上还原型谷胱甘肽的含量。单点法测定相对比较便捷,而动力学法测定则相对比较精确。注意:由于1个GSSG分子反应后可以还原成2个GSH分子,所以GSSG的浓度如果换算成GSH的浓度时需乘以2,例如完全清除样品中内源GSH的情况下,GSSG的浓度为5μM,则相当于GSH的浓度为10μM。
b.动力学测定法:先根据不同时间点测定得到的吸光度值计算出ΔA412/min。然后以标准品的浓度为横坐标,以ΔA412/min为纵坐标,做出标准曲线。根据样品的ΔA412/min,对照标准曲线就可以计算出测定时样品中总谷胱甘肽或GSSG的含量。
c.同时根据样品的稀释倍数以及最初样品的使用量,可以计算出每毫克组织或细胞中的总谷胱甘肽或GSSG的含量。对于细胞样品,也可以根据最初细胞的使用数量,然后另外取一定数量的细胞裂解后测定蛋白浓度,从而计算出细胞样品的蛋白量,最后计算出每毫克蛋白中总谷胱甘肽或GSSG的含量。
d.根据测定得到的总谷胱甘肽的含量和GSSG的含量就可以计算出GSH的含量。
计算公式为:GSH=Total Glutathione-GSSG×2 (注意:Total Glutathione为通过标准曲线计算得到的GSSG浓度乘以2,同时清除GSH后得到的GSSG也要乘以2,因为1个GSSG分子在反应后可以还原成2个GSH分子)。例如通过本试剂盒测定的总谷胱甘肽(Total Glutathione)的浓度是15μM (即在测定总谷胱甘肽时通过标准曲线计算得到的GSSG浓度为7.5μM,乘以2即为总谷胱甘肽浓度),测定的GSSG的浓度是1.2μM (即在单独测定GSSG含量时通过标准曲线计算得到的GSSG浓度为1.2μM),那么样品中GSH的浓度为15-1.2×2=12.6μM。

相关产品:

产品编号 产品名称 包装
S0052 总谷胱甘肽检测试剂盒 100次
S0052B 蛋白去除试剂S 3g
S0053 GSH和GSSG检测试剂盒 共100次
S0054 蛋白去除试剂M 5g
S0055 谷胱甘肽还原酶检测试剂盒 100次
S0056 谷胱甘肽过氧化物酶检测试剂盒 100次
S0058 总谷胱甘肽过氧化物酶检测试剂盒 100次

GSH和GSSG检测试剂盒(S0053)

相关产品请点击如下按钮:
活性氧相关

使用本产品的文献:
1. Ji LL, Chen Y, Wang ZT.
Yamaoka Ligularia base of HEK293 cells glutathione content and the impact of related activity.
INT J TOXICOL . 2007 Jun; 21(3):205-7. (IF 1.255)

2. Jia JH, Wang Y, Cao YB, Gao PH, Jia XM, Ma ZP, Xu YG, Dai BD, Jiang YY.
CaIPF7817 is involved in the regulation of redox homeostasis in Candida albicans.
BIOCHEM BIOPH RES CO . 2007 Jul 20;359(1):163-7. (IF 2.985)

3. Wang B, Feng WY, Zhu MT, Wang Y, Wang M, Gu YQ, Ouyang H, Wang HJ, Li M, Zhao YL, Chai ZF, Wang HF.
Neurotoxicity of low-dose repeatedly intranasal instillation of nano- and submicron-sized ferric oxide particles in mice.
J Nanopart Res . 2007 Sep;118(3):233-43. (IF 2.132)

4. Zhu MT, Feng WY, Wang B, Wang TC, Gu YQ, Wang M, Wang Y, Ouyang H, Zhao YL, Chai ZF.
Comparative study of pulmonary responses to nano- and submicron-sized ferric oxide in rats.
Toxicology . 2008 May 21;247(2-3):102-11. (IF 4.099)

5. Bi J, Jiang B, Liu JH, Zhang XL, An LJ.
Protective effects of catalpol against H(2)O(2)-induced oxidative stress in astrocytes primary cultures.
Neurosci Lett . 2008 Sep 19;442(3):224-7. (IF 2.274)

6. Fan J, Cai H, Yang S, Yan L, Tan W.
Comparison between the effects of normoxia and hypoxia on antioxidant enzymes and glutathione redox state in ex vivo culture of CD34(+) cells.
COMP BIOCHEM PHYS B . 2008 Oct;151(2):153-8. (IF 2.219)

7. Wang L, Yan B, Liu N, Li Y, Wang Q.
Effects of cadmium on glutathione synthesis in hepatopancreas of freshwater crab, Sinopotamon yangtsekiense.
Chemosphere . 2008 Dec;74(1):51-6. (IF 5.778)

8. Liu Z, Zhang X, Bai J, Suo B, Xu P, Wang L.
Exogenous paraquat changes antioxidant enzyme activities and lipid peroxidation in drought-stressed cucumber leaves.
Scientia Horticulturae . 2009;121:138-43. (IF 1.961)

9. LIU JJ, LIN SH, XU PL, WANG XJ, BAI JG.
Effects of Exogenous Silicon on the Activities of Antioxidant Enzymes and Lipid Peroxidation in Chilling-Stressed Cucumber Leaves.
AGR SCI CHINA . 2009; 8(9): 1075-86. (IF 0.82)

10. Zhang P, Xua Y, Li L, Jiang Q, Wang M, Jin L.
In vitro protective effects of pyrroloquinoline quinone on methylmercury-induced neurotoxicity.
Environmental Toxicology and Pharmacology . 2009 Jan;27(1):103-10. (IF 3.061)

11. Dong HS, Li L, Song ZH, Tang J, Xu B, Zhai XW, Sun LL, Zhang P, Li ZB, Pan QJ, Shi QH, Shen W.
Premeiotic fetal murine germ cells cultured in vitro form typical oocyte-like cells but do not progress through meiosis.
Theriogenology . 2009 Jul 15;72(2):219-31. (IF 2.094)

12. Sun ZW, Zhang L, Zhu SJ, Chen WC, Mei B.
Excitotoxicity effects of glutamate on human neuroblastoma SH-SY5Y cells via oxidative damage.
Neurosci Bull . 2010;26(1):8-16. (IF 4.326)

13. Cao X, Xiao H, Zhang Y, Zou L, Chu Y, Chu X.
1, 5-Dicaffeoylquinic acid-mediated glutathione synthesis through activation of Nrf2 protects against OGD/reperfusion-induced oxidative stress in astrocytes.
Brain Res . 2010;1347:142-8. (IF 2.733)

14. Sun L, Xu S, Zhou M, Wang C, Wu Y, Chan P.
Effects of cysteamine on MPTP-induced dopaminergic neurodegeneration in mice.
Brain Res . 2010;1335:74-82. (IF 2.733)

15. Huang JZ, Chen YZ, Su M, Zheng HF, Yang YP, Chen J, Liu CF.
dl-3-n-Butylphthalide prevents oxidative damage and reduces mitochondrial dysfunction in an MPP(+)-induced cellular model of Parkinson’s disease.
Neurosci Lett . 2010;475(2):89-94. (IF 2.274)

16. Liu ZJ, Guo YK, Bai JG.
Exogenous hydrogen peroxide changes antioxidant enzyme activity and protects ultrastructure in leaves of two cucumber ecotypes under osmotic stress.
JOURNAL OF PLANT GROWTH REGULATION .2010;29(7):171-83. (IF 2.179)

17. Xu JY, Su YY, Cheng JS, Li SX, Liu RL, Li WX, Xu GT, Li QN.
Protective effects of fullerenol on carbon tetrachloride-induced acute hepatotoxicity and nephrotoxicity in rats.
Sciencedirect Carbon . 2010 Apr;48(5):1388-96.

18. Wang W, Feng MF, Lin Z.
The Glutathione and Metallothionein Contents Variation in Earthworm Exposed by AHTN.
Bioinformatics and Biomedical Engineering (iCBBE) .2010 Jun 18-20;1-4.

19. Liu J, Zhou J, An W, Lin Y, Yang Y, Zang W.
Apocynin attenuates pressure overload-induced cardiac hypertrophy in rats by reducing levels ofreactive oxygen species.
CAN J PHYSIOL PHARM . 2010 Jul;88(7):745-52. (IF 1.946)

20. Yang CR, Miao DQ, Zhang QH, Guo L, Tong JS, Wei Y, Huang X, Hou Y, Schatten H, Liu Z, Sun QY.
Short-term preservation of porcine oocytes in ambient temperature: novel approaches.
PLoS One . 2010 Dec 7;5(12):e14242. (IF 2.74)

21. Hu L, Chen L, Yang G, Li L, Sun H, Chang Y, Tu Q, Wu M, Wang H.
HBx sensitizes cells to oxidative stress-induced apoptosis by accelerating the loss of Mcl-1 proteinvia caspase-3 cascade.
Mol Cancer . 2011 Apr 20;10:43. (IF 15.302)

22. Ding B, Yuan L, Yu H, Li L, Ma W, Bi Y, Feng J, Xiao R.
Genistein and folic acid prevent oxidative injury induced by β-amyloid peptide.
BASIC CLIN PHARMACOL . 2011 May;108(5):333-40. (IF 2.651)

23. Li Q, Yu B, Gao Y, Dai AH, Bai JG.
Cinnamic acid pretreatment mitigates chilling stress of cucumber leaves through alteringantioxidant enzyme activity.
J Plant Physiol . 2011 Jun 15;168(9):927-34. (IF 3.013)

24. Zhang XL,Jia XF, Yu B, Gao Y, Bai JG.
Exogenous hydrogen peroxide influences antioxidant enzyme activity and lipid peroxidation in cucumber leaves at low light.
Scientia Horticulturae . 2011Jul;129(4):656-2. (IF 1.961)

25. Zhang J, Zhao QZ, Duan GL, Huang YC.
Influence ofsulphuronarsenicaccumulationandmetabolisminriceseedlings.
ENVIRON EXP BOT .2011 Aug;72(1):34-40. (IF 4.027)

26. Sun WJ, Nie YX, Gao Y, Dai AH, Bai JG.
Exogenous cinnamic acid regulates antioxidant enzyme activity and reduces lipid peroxidation in drought-stressed cucumber leaves.
ACTA PHYSIOLOGIAE PLANTARUM .2012;34(2):641-55. (IF 1.608)

27. Zhang B, Chen N, Chen H, Wang Z, Zheng Q.
The critical role of redox homeostasis in shikonin-induced HL-60 cell differentiation via unique modulation of theNrf2/ARE pathway.
Oxid Med Cell Longev . 2012;2012:781516. (IF 5.076)

28. Wang J, Hou Y, Dong L, Niu X, Fan Y.
Influence of TiO2 nanoparticles on glutathione in rat synovial cell line RSC-364.
World Congress on Medical Physics and Biomedical Engineering . 2012 May 26-31;39:75-8.

29. Dai A, Nie Y, Yu B, Li Q, Lu L,Bai J.
Cinnamic acid pretreatment enhances heat tolerance of cucumber leaves through modulating antioxidant enzyme activity.
Environmental and Experimental Botany .2012 Jul;79:1-10. (IF 3.712)

30. Zhong WX, Wang YB, Peng L, Ge XZ, Zhang J, Liu SS, Zhang XN, Xu ZH, Chen Z, Luo JH.
Lanthionine synthetase C-like protein 1 interacts with and inhibits cystathionine β-synthase: a target for neuronalantioxidant defense.
J Biol Chem . 2012 Oct 5;287(41):34189-201. (IF 4.238)

31. Jia P, Xu YJ, Zhang ZL, Li K, Li B, Zhang W, Yang H.
Ferric ion could facilitate osteoclast differentiation and bone resorption through the production ofreactive oxygen species.
J Orthop Res . 2012 Nov;30(11):1843-52. (IF 2.728)

32. Zhang J, Li D, Sun W, Wang X, Bai J.
Exogenous p-hydroxybenzoic acid regulates antioxidant enzyme activity and mitigates heat stress of cucumber leaves.
Scientia Horticulturae .2012 Dec 4;148:235- 45. (IF 1.961)

33. Zhang L, Zhang J, Zhao B, Zhao-Wilson X.
Quinic acid could be a potential rejuvenating natural compound by improving survival of Caenorhabditis elegansunder deleterious conditions.
REJUV RES . 2012 Dec;15(6):573-83. (IF 3.811)

34. Khan M, Li T, Ahmad Khan MK, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T.
Alantolactone induces apoptosis in HepG2 cells through GSH depletion, inhibition of STAT3 activation, andmitochondrial dysfunction.
Biomed Res Int . 2013;2013:719858. (IF 2.276)

35. Jiang Q, Zhou Z, Wang L, Shi X, Wang J, Yue F, Yi Q, Yang C, Song L.
The immunomodulation of inducible nitric oxide in scallop Chlamys farreri.
FISH SHELLFISH IMMUN . 2013 Jan;34(1):100-8. (IF 3.298)

36. Wang J, Jiang Z, Ji J, Wang X, Wang T, Zhang Y, Tai T, Chen M, Sun L, Li X, Zhang L.
Gene expression profiling and pathway analysis of hepatotoxicity induced by triptolide in Wistar rats.
Food Chem Toxicol . 2013 Apr 29;58C:495-505. (IF 4.679)

37. Ren C, Li Y, Han R, Gao D, Li W, Shi J, Hoogewijs D, Braeckman BP, De Henau S, Lu Y, Qu W, Gao Y, Wu Y, Li Z, Liu H, Wang Z, Zhang C.
GLB-13 is associated with oxidative stress resistance in caenorhabditis elegans.
IUBMB Life . 2013 May;65(5):423-34. (IF 3.244)

38. Jing C, Li X, Zhang J, Wang J.
Responses of the Antioxidant System in QGY-7701 Cells to the Cytotoxicity and Apoptosis Induced by 1-Octyl-3-methylimidazolium Chloride.
J BIOCHEM MOL TOXIC . 2013 Jun;27(6):330-6. (IF 3.606)

39. Dai BD, Wang Y, Zhao LX, Li DD, Li MB, Cao YB, Jiang YY.
Cap1p attenuates the apoptosis of Candida albicans.
FEBS J . 2013 Jun;280(11):2633-43. (IF 4.392)

40. Ma WW, Hou CC, Zhou X, Yu HL, Xi YD, Ding J, Zhao X, Xiao R.
Genistein alleviates the mitochondria-targeted DNA damage induced by β-amyloid peptides 25-35 in C6 gliomacells.
Neurochem Res . 2013 Jul;38(7):1315-23. (IF 3.038)

41. Zhang L, Wang L, Hu Y, Liu Z, Tian Y, Wu X, Zhao Y, Tang H, Chen C, Wang Y.
Selective metabolic effects of gold nanorods on normal and cancer cells and their application in anticancer drugscreening.
Biomaterials . 2013 Sep;34(29):7117-26. (IF 10.317)

42. Liu L, Wang P, Liang C, He D, Yu Y, Liu X.
Distinct effects of Nampt inhibition on mild and severe models of lipopolysaccharide-induced myocardialimpairment.
Int Immunopharmacol . 2013 Oct;17(2):342-9. (IF 3.943)

43. Mo C, Wang L, Zhang J, Numazawa S, Tang H, Tang X, Han X, Li J, Yang M, Wang Z, Wei D, Xiao H.
The crosstalk between Nrf2 and AMPK signal pathways is important for the anti-inflammatory effect of berberine in LPS-stimulated macrophages and endotoxin-shocked mice.
ANTIOXID REDOX SIGN . 2014 Feb 1;20(4):574-88. (IF 6.323)

44. Xu W, Wang S, Chen Q, Zhang Y, Ni P, Wu X, Zhang J, Qiang F, Li A, Røe OD, Xu S, Wang M, Zhang R, Zhou J.
TXNL1-XRCC1 pathway regulates cisplatin-induced cell death and contributes to resistance in human gastric cancer.
Cell Death Dis . 2014 Feb 13;5:e1055. (IF 6.304)

45. Meng G, Xia M, Xu C, Yuan D, Schnurr M, Wei J.
Multifunctional antitumor molecule 5′-triphosphate siRNA combining glutaminase silencing and RIG-I activation.
Int J Cancer . 2014 Apr 15;134(8):1958-71. (IF 5.145)

46. He X, Song W, Liu C, Chen S, Hua J.
Rapamycin inhibits acrolein-induced apoptosis by alleviating ROS-driven mitochondrial dysfunction in male germ cells.
CELL PROLIFERAT . 2014 Apr;47(2):161-71. (IF 5.753)

47. Hou Y, Lai M, Chen X, Li J, Hu Y, Luo Z, Ding X, Cai K.
Effects of mesoporous SiO2 , Fe3 O4 , and TiO2 nanoparticles on the biological functions of endothelial cells in vitro.
J Biomed Mater Res A .2014 Jun;102(6):1726-36. (IF 3.525)

48. Zhou H, Wang X, Zhou Y, Yao H, Ahmad F.
Evaluation of the toxicity of ZnO nanoparticles to Chlorella vulgaris by use of the chiral perturbation approach.
Anal Bioanal Chem . 2014 Jun;406(15):3689-95. (IF 3.637)

49. Wang Y, Zhao W, Hao J, Xu W, Luo Y, Wu W, Yang Z, Liang Z, Huang K.
Changes in biosynthesis and metabolism of glutathione upon ochratoxin A stress in Arabidopsis thaliana.
PLANT PHYSIOL BIOCH . 2014 Jun;79:10-8. (IF 3.72)

50. Wang C, Chen H, Zhang J, Hong Y, Ding X, Ying W.
Malate-aspartate shuttle mediates the intracellular ATP levels, antioxidation capacity and survival of differentiated PC12 cells.
Int J Physiol Pathophysiol Pharmacol . 2014 Jul 12;6(2):109-14.

51. Diao Y, Xu H, Li G, Yu A, Yu X, Hu W, Zheng X, Li S, Wang Y, Hu Z.
Cloning a glutathione peroxidase gene from Nelumbo nucifera and enhanced salt tolerance by overexpressing in rice.
Mol Biol Rep . 2014 Aug;41(8):4919-27. (IF 1.402)

52. Xie JM, Li B, Yu HP, Gao QG, Li W, Wu HR, Qin ZH.
TIGAR has a dual role in cancer cell survival through regulating apoptosis and autophagy.
Cancer Res . 2014 Sep 15;74(18):5127-38. (IF 9.727)

53. Zhang B, Wang XQ, Chen HY, Liu BH.
Involvement of the Nrf2 Pathway in the Regulation of Pterostilbene-Induced Apoptosis in HeLa Cells via ER Stress.
J Pharmacol Sci . 2014 Nov 20;126(3):216-229. (IF 2.835)

54. Zhang C, Zhou Z, Zhi X, Ma Y, Wang K, Wang Y, Zhang Y, Fu H, Jin W, Pan F, Cui D.
Insights into the distinguishing stress-induced cytotoxicity of chiral gold nanoclusters and the relationship with GSTP1.
Theranostics . 2015 Jan 1;5(2):134-49. (IF 8.579)

55. Ma YC, Su N, Shi XJ, Zhao W, Ke Y, Zi X, Zhao NM, Qin YH, Zhao HW, Liu HM.
Jaridonin-induced G2/M phase arrest in human esophageal cancer cells is caused by reactive oxygen species-dependent Cdc2-tyr15 phosphorylation via ATM-Chk1/2-Cdc25C pathway.
TOXICOL APPL PHARM  . 2015 Jan 15;282(2):227-36. (IF 3.616)

56. Shi H, Jiang C, Ye T, Tan D, Reiter RJ, Zhang H, Liu R, Chan Z.
Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of improved abiotic stress resistance in bermudagrass [Cynodon dactylon (L). Pers.] by exogenous melatonin.
J Exp Bot .2015 Feb;66(3):681-94. (IF 5.908)

57. Liu H, Li Q, Cheng X, Wang H, Wang G, Hao H.
UDP-glucuronosyltransferase 1A determinates intracellular accumulation and anti-cancer effect of β-lapachone in human colon cancer cells.
PLoS One . 2015 Feb 18;10(2):e0117051. (IF 2.74)

58. Wang G, Wang H, Xiong X, Chen S, Zhang D.
Mitochondria thioredoxin’s backup role in oxidative stress resistance in Trichoderma reesei.
Microbiol Res . 2015 Feb;171:32-8. (IF 3.97)

59. Jiang F, Chen L, Yang YC, Wang XM, Wang RY, Li L, Wen W, Chang YX, Chen CY, Tang J, Liu GM, Huang WT, Xu L, Wang HY.
CYP3A5 Functions as a Tumor Suppressor in Hepatocellular Carcinoma by Regulating mTORC2/Akt Signaling.
Cancer Res . 2015 Apr 1;75(7):1470-81. (IF 9.727)

60. She T, Qu L, Wang L, Yang X, Xu S, Feng J, Gao Y, Zhao C, Han Y, Cai S, Shou C.
Sarsaparilla (Smilax Glabra Rhizome) Extract Inhibits Cancer Cell Growth by S Phase Arrest, Apoptosis, and Autophagy via Redox-Dependent ERK1/2 Pathway.
CANCER PREV RES . 2015 May;8(5):464-74. (IF 3.473)

61. Shi H, Ye T, Han N, Bian H, Liu X, Chan Z.
Hydrogen sulfide regulates abiotic stress tolerance and biotic stress resistance in Arabidopsis.
J Integr Plant Biol .2015 Jul;57(7):628-40. (IF 4.885)

62. Du Z, Jia XL, Wang Y, Wu T, Han ZH, Zhang XZ.
Redox homeostasis and reactive oxygen species scavengers shift during ontogenetic phase changes in apple.
Plant Sci . 2015 Jul;236:283-94. (IF 3.591)

63. Zheng CP, Han L, Hou WJ, Tang J, Wen YH, Fu R, Wang YJ, Wen WP.
MicroRNA-9 suppresses the sensitivity of CNE2 cells to ultraviolet radiation.
Mol Med Rep . 2015 Aug;12(2):2367-73. (IF 2.1)

64. Zheng L, Meng Y, Ma J, Zhao X, Cheng T, Ji J, Chang E, Meng C, Deng N, Chen L, Shi S, Jiang Z.
Transcriptomic analysis reveals importance of ROS and phytohormones in response to short-term salinity stress in Populus tomentosa.
Front Plant Sci . 2015 Sep 15;6:678. (IF 4.402)

65. Qiu M, Chen L, Tan G, Ke L, Zhang S, Chen H, Liu J.
A reactive oxygen species activation mechanism contributes to JS-K-induced apoptosis in human bladder cancer cells.
SCI REP-UK . 2015 Oct 13;5:15104. (IF 3.998)

66. Xu Y, Zhou X, Shi C, Wang J, Wu Z.
α-Lipoic acid protects against the oxidative stress and cytotoxicity induced by cadmium in HepG2 cells through regenerating glutathione regulated by glutamate-cysteine ligase.
TOXICOL MECH METHOD . 2015 Oct;25(8):596-603. (IF 2.295)

67. Zhang J, Cao M, Yang W, Sun F, Xu C, Yin L, Pu Y.
Inhibition of Glucose-6-Phosphate Dehydrogenase Could Enhance 1,4-Benzoquinone-Induced OxidativeDamage in K562 Cells.
Oxid Med Cell Longev . 2016;2016:3912515. (IF 5.076)

68. Liu Z, Gan L, Chen Y, Luo D, Zhang Z, Cao W, Zhou Z, Lin X, Sun C.
Mark4 promotes oxidative stress and inflammation via binding to PPARγ and activating
SCI REP-UK . 2016 Feb 18;6:21382. (IF 3.998)

69. Deng C, Zhang B, Zhang S, Duan C, Cao Y, Kang W, Yan H, Ding X, Zhou F, Wu L, Duan G, Shen S, Xu G, Zhang W, Chen M, Huang S,Zhang X, Lv Y, Ling T, Wang L, Zou X.
Low nanomolar concentrations of Cucurbitacin-I induces G2/M phase arrest and apoptosis by perturbingredox homeostasis in gastric cancer cells in vitro and in vivo.
Cell Death Dis . 2016 Feb 18;7:e2106. (IF 6.304)

70. Deng C, Zhang B, Zhang S, Duan C, Cao Y, Kang W, Yan H, Ding X, Zhou F, Wu L, Duan G, Shen S, Xu G, Zhang W, Chen M, Huang S, Zhang X, Lv Y, Ling T, Wang L, Zou X.
Low nanomolar concentrations of Cucurbitacin-I induces G2/M phase arrest and apoptosis by perturbing redox homeostasis in gastric cancer cells in vitro and in vivo.
Cell Death Dis . 2016 Feb 18;7:e2106. (IF 6.304)

71. Liu X, Fu Z, Wu Y, Hu X Jr, Zhu T Jr, Jin C Jr.
Neuroprotective effect of hydrogen sulfide on acute cauda equina injury in rats.
Spine J . 2016 Mar;16(3):402-7. (IF 3.191)

72. Wang X, Liang Z, Hou J, Bao X, Shen Y.
Identification and functional evaluation of the reductases and dehydrogenases from
BMC Biotechnol . 2016 Apr 1;16:31. (IF 2.312)

73. Lu MC, Ji JA, Jiang YL, Chen ZY, Yuan ZW, You QD, Jiang ZY.
An inhibitor of the Keap1-Nrf2 protein-protein interaction protects NCM460 colonic
SCI REP-UK . 2016 May 24;6:26585. (IF 3.998)

74. Fang Y, Wang J, Xu L, Cao Y, Xu F, Yan L, Nie M, Yuan N, Zhang S, Zhao R, Wang H, Wu M, Zhang X, Wang J.
Autophagy maintains ubiquitination-proteasomal degradation of Sirt3 to limit oxidative stress in K562leukemia cells.
ONCOTARGET . 2016 Jun 14;7(24):35692-35702. (IF 5.168)

75. Fan XY, Chen XY, Liu YJ, Zhong HM, Jiang FL, Liu Y.
Oxidative stress-mediated intrinsic apoptosis in human promyelocytic leukemia HL-60 cells induced byorganic arsenicals.
SCI REP-UK . 2016 Jul 19;6:29865. (IF 3.998)

76. Zhou Q, Chen B, Wang X, Wu L, Yang Y, Cheng X, Hu Z, Cai X, Yang J, Sun X, Lu W, Yan H, Chen J, Ye J, Shen J, Cao P.
Sulforaphane protects against rotenone-induced neurotoxicity in vivo: Involvement of the mTOR, Nrf2, andautophagy pathways.
SCI REP-UK . 2016 Aug 24;6:32206. (IF 3.998)

77. Guo L, Lv G, Qiu L, Yang H, Zhang L, Yu H, Zou M, Lin J.
Insights into anticancer activity and mechanism of action of a ruthenium(II) complex
Eur J Pharmacol . 2016 Sep 5;786:60-71. (IF 3.263)

78. Liu Q, Sun Y, Lv Y, Le Z, Xin Y, Zhang P, Liu Y.
TERT alleviates irradiation-induced late rectal injury by reducing hypoxia-induced ROS levels through theactivation of NF-κB and autophagy.
Int J Mol Med . 2016 Sep;38(3):785-93. (IF 3.098)

79. Guo H, Zhang N, Liu D, Wang P, Ma X.
Inhibitory effect on the proliferation of human heptoma induced by cell-permeable
Biomed Pharmacother . 2016 Oct;83:1379-1386. (IF 4.545)

80. Yuan-Jing F, Wei W, Jian-Ping L, Yu-Xia J, Zi-Ling D.
Genistein promotes the metabolic transformation of acetaminophen to glucuronic acid in human L-O2,HepG2 and Hep3b cells via the Nrf2/Keap1 pathway.
Food Funct . 2016 Nov 9;7(11):4683-4692. (IF 4.171)

81. Tao J, Shen X, Ai Y, Han X.
Tea polyphenols protect against ischemia/reperfusion-induced liver injury in mice through anti-oxidativeand anti-apoptotic properties.
Exp Ther Med . 2016 Nov;12(5):3433-3439. (IF 1.785)

82. Wang D, Meng G, Zheng M, Zhang Y, Chen A, Wu J, Wei J.
The Glutaminase-1 Inhibitor 968 Enhances Dihydroartemisinin-Mediated Antitumor Efficacy in HepatocellularCarcinoma Cells.
PLoS One . 2016 Nov 11;11(11):e0166423. (IF 2.74)

83. Xu Y, Zhu J, Hu X, Wang C, Lu D, Gong C, Yang J, Zong L.
CLIC1 Inhibition Attenuates Vascular Inflammation, Oxidative Stress, and Endothelial Injury.
PLoS One . 2016 Nov 18;11(11):e0166790. (IF 2.74)

84. Liang W, Cai A, Chen G, Xi H, Wu X, Cui J, Zhang K, Zhao X, Yu J, Wei B, Chen L.
Shikonin induces mitochondria-mediated apoptosis and enhances chemotherapeutic sensitivity of gastriccancer through reactive oxygen species.
SCI REP-UK . 2016 Dec 1;6:38267. (IF 3.998)

85. Li X, Fan XX, Jiang ZB, Loo WT, Yao XJ, Leung EL, Chow LW, Liu L.
Shikonin inhibits gefitinib-resistant non-small cell lung cancer by inhibiting TrxR and activating the EGFRproteasomal degradation pathway.
Pharmacol Res . 2017 Jan;115:45-55. (IF 5.893)

86. Li X, Fan XX, Jiang ZB, Loo WT, Yao XJ, Leung EL, Chow LW, Liu L.
Shikonin inhibits gefitinib-resistant non-small cell lung cancer by inhibiting TrxR and activating the EGFR proteasomal degradation pathway.
Pharmacol Res . 2017 Jan;115:45-55. (IF 5.893)

87. Lu S, Zhang Y, Zhong S, Gao F, Chen Y, Li W, Zheng F, Shi G.
N-n-butyl Haloperidol Iodide Protects against Hypoxia/Reoxygenation Injury in CardiacMicrovascular EndothelialCells by Regulating the ROS/MAPK/Egr-1 Pathway.
Front Pharmacol . 2017 Jan 5;7:520. (IF 4.225)

88. Sun X, Wang W, Chen J, Cai X, Yang J, Yang Y, Yan H, Cheng X, Ye J, Lu W, Hu C, Sun H, Pu J, Cao P.
The Natural Diterpenoid Isoforretin A Inhibits Thioredoxin-1 and Triggers Potent ROS-Mediated AntitumorEffects.
Cancer Res . 2017 Feb 15;77(4):926-936. (IF 9.727)

89. Wang Y, Gu YH, Liu M, Bai Y, Wang HL.
Fluoxetine protects against methamphetamine induced lung inflammation by suppressing oxidative stressthrough the SERT/p38 MAPK/Nrf2 pathway in rats.
Mol Med Rep . 2017 Feb;15(2):673-680. (IF 2.1)

90. Sun X, Wang W, Chen J, Cai X, Yang J, Yang Y, Yan H, Cheng X, Ye J, Lu W, Hu C, Sun H, Pu J, Cao P.
The Natural Diterpenoid Isoforretin A Inhibits Thioredoxin-1 and Triggers Potent ROS-MediatedAntitumor Effects.
Cancer Res . 2017 Feb 15;77(4):926-936. (IF 9.727)

91. Wang Y, Gu YH, Liu M, Bai Y, Wang HL.
Fluoxetine protects against methamphetamine induced lung inflammation by suppressing oxidative stress through the SERT/p38 MAPK/Nrf2 pathway in rats.
Mol Med Rep . 2017 Feb;15(2):673-680. (IF 2.1)

92. Jiang Y, Cao Y, Wang Y, Li W, Liu X, Lv Y, Li X, Mi J.
Cysteine transporter SLC3A1 promotes breast cancer tumorigenesis.
Theranostics . 2017 Feb 26;7(4):1036-1046. (IF 8.579)

93. Zhang Z, Chen J, Li B, He C, Chen Y, Tian S.
Influence of Oxidative Stress on Biocontrol Activity of Cryptococcus laurentii against Blue Mold on Peach Fruit.
Front Microbiol . 2017 Feb 2;8:151. (IF 4.235)

94. Qiu M, Chen L, Tan G, Ke L, Zhang S, Chen H, Liu J.
JS-K promotes apoptosis by inducing ROS production in human prostate cancer cells.
Oncol Lett . 2017 Mar;13(3):1137-1142. (IF 2.311)

95. Zhang XL, Yuan YH, Shao QH, Wang ZZ, Zhu CG, Shi JG, Ma KL, Yan X, Chen NH.
DJ-1 regulating PI3K-Nrf2 signaling plays a significant role in bibenzyl compound 20C-mediatedneuroprotection against rotenone-induced oxidative insult.
Toxicol Lett . 2017 Apr 5;271:74-83. (IF 3.569)

96. Shi H, Li Y, Ren X, Zhang Y, Yang Z, Qi C.
A novel quinazoline-based analog induces G2/M cell cycle arrest and apoptosis in human A549lung cancer cells via a ROS-dependent mechanism.
BIOCHEM BIOPH RES CO . 2017 Apr 29;486(2):314-320. (IF 2.985)

97. Zhang J, Zhou X, Wu W, Wang J, Xie H, Wu Z.
Regeneration of glutathione byα-lipoic acid via Nrf2/ARE signaling pathway alleviates cadmium-induced HepG2 cell toxicity.
ENVIRON TOXICOL CHEM . 2017 Apr;51:30-37. (IF 3.152)

98. Li X, Li X, Lu J, Huang Y, Lv L, Luan Y, Liu R, Sun R.
Saikosaponins induced hepatotoxicity in mice via lipid metabolism dysregulation and oxidativestress: a proteomic study.
BMC COMPLEM ALTERN M . 2017 Apr 19;17(1):219. (IF 2.833)

99. Xu J, Bian X, Liu Y, Hong L, Teng T, Sun Y, Xu Z.
Adenosine A2 receptor activation ameliorates mitochondrial oxidative stress upon reperfusion throughthe posttranslational modification of NDUFV2 subunit of complex I in the heart.
FREE RADICAL BIO MED . 2017 May;106:208-218. (IF 6.17)

100. Guo J, Xu B, Han Q, Zhou H, Xia Y, Gong C, Dai X, Li Z, Wu G.
Ferroptosis:A Novel Anti-Tumor Action for Cisplatin.
Cancer Res Treat . 2017 May 10. (IF 3.761)

101. Xu X, Xia L, Chen W, Huang Q.
Detoxification of hexavalent chromate by growing Paecilomyces lilacinus XLA.
Environ Pollut. 2017 Jun;225:47-54. (IF 6.792)

102. Lin XH, Pan JB, Zhang XJ.
WITHDRAWN: Anti-inflammatory and anti-oxidant effects of apigetrin on LPS-induced acute lung injury by regulating Nrf2 and AMPK pathways.
BIOCHEM BIOPH RES CO . 2017 Jul 13. pii: S0006-291X(17)31413-4. (IF 2.985)

103. Liu X, Liu H, Zhai Y, Li Y, Zhu X, Zhang W.
Laminarin protects against hydrogen peroxide-induced oxidative damage in MRC-5 cells possiblyvia regulating NRF2.
PeerJ . 2017 Jul 31;5:e3642. (IF 2.379)

104. Jiang LL, Zhao DS, Fan YX, Yu Q, Li P, Li HJ.
Detection of Emodin Derived Glutathione Adduct in Normal Rats Administered with Large Dosage of Polygoni Multiflori Radix.
Front Pharmacol . 2017 Jul 6;8:446. (IF 4.225)

105. Duan M, Zhang R, Zhu F, Zhang Z, Gou L, Wen J, Dong J, Wang T.
A Lipid-Anchored NAC Transcription Factor Is Translocated intothe Nucleus and ActivatesGlyoxalase I Expression during Drought Stress.
Plant Cell . 2017 Jul;29(7):1748-1772. (IF 9.618)

106. Duan M, Zhang R, Zhu F, Zhang Z, Gou L, Wen J, Dong J, Wang T.
A Lipid-Anchored NAC Transcription Factor Is Translocated into the Nucleus and Activates Glyoxalase I Expression during Drought Stress.
Plant Cell . 2017 Jul;29(7):1748-1772. (IF 9.618)

107. He JH, Yan M, Zuo H, Niu S, Yuan J, Weng SP, He J, Xu X.
High reduced/oxidized glutathione ratio in infectious spleen and kidney necrosis virus-infectedcells contributes to degradation of VP08R multimers.
Vet Microbiol . 2017 Aug;207:19-24. (IF 3.03)

108. Qiu M, Ke L, Zhang S, Zeng X, Fang Z, Liu J.
JS-K,a GST-activated nitric oxide donor prodrug, enhances chemo-sensitivity in renal carcinomacells and prevents cardiac myocytes toxicity induced by Doxorubicin.
CANCER CHEMOTH PHARM . 2017 Aug;80(2):275-286. (IF 2.967)

109. Meng W, Pei Z, Feng Y, Zhao J, Chen Y, Shi W, Xu Q, Lin F, Sun M, Xiao K.
Neglected role of hydrogen sulfide in sulfur mustard poisoning: Keap1 S-sulfhydration and subsequent Nrf2 pathway activation.
SCI REP-UK . 2017 Aug 25;7(1):9433. (IF 3.998)

110. Zhang GM, Deng MT, Lei ZH, Wan YJ, Nie HT, Wang ZY, Fan YX, Wang F, Zhang YL.
Effects of NRF1 on steroidogenesis and apoptosis in goat luteinized granulosa cells.
Reproduction . 2017 Aug;154(2):111-122. (IF 3.206)

111. Wu H, Yu W, Meng F, Mi J, Peng J, Liu J, Zhang X, Hai C, Wang X.
Polychlorinated biphenyls-153 induces metabolic dysfunction through activation of ROS/NF-κB signaling via downregulation of HNF1b.
Redox Biol . 2017 Aug;12:300-310. (IF 9.986)

112. Zhao J, Fu B, Peng W, Mao T, Wu H, Zhang Y.
Melatonin protect the development of preimplantation mouse embryos from sodium fluoride-induced oxidative injury.
ENVIRON TOXICOL CHEM . 2017 Sep;54:133-141. (IF 3.152)

113. Huang L, Zhang K, Guo Y, Huang F, Yang K, Chen L, Huang K, Zhang F, Long Q, Yang Q.
Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts.
SCI REP-UK . 2017 Sep 20;7(1):11989. (IF 3.998)

114. Chen H, Tang X, Zhou B, Zhou Z, Xu N, Wang Y.
A ROS-mediated mitochondrial pathway and Nrf2 pathway activation are involved in BDE-47induced apoptosis in Neuro-2a cells.
Chemosphere . 2017 Oct;184:679-686. (IF 5.778)

115. Feng RB, Wang Y, He C, Yang Y, Wan JB.
Gallic acid,a natural polyphenol, protects against tert-butyl hydroperoxide-induced hepatotoxicity by activating ERK-Nrf2-Keap1-mediated antioxidative response.
Food Chem Toxicol . 2017 Oct 21. pii: S0278-6915(17)30631-2. (IF 4.679)

116. He X, Wu C, Cui Y, Zhu H, Gao Z, Li B, Hua J, Zhao B.
The aldehyde group of gossypol induces mitochondrial apoptosis via ROS-SIRT1-p53-PUMApathway in male germline stem cell.
ONCOTARGET . 2017 Oct 24;8(59):100128-100140. (IF 5.168)

117. Wang XY, Li YL, Wang HY, Zhu M, Guo D, Wang GL, Gao YT, Yang Z, Li T, Yang CY, Chen YM.
Propofol inhibits invasion and proliferation of C6 glioma cells by regulating the Ca2+ permeableAMPA receptor-system xc- pathway.
Toxicol In Vitro . 2017 Oct;44:57-65. (IF 2.959)

118. Huang R, Li X, Yu Y, Ma L, Liu S, Zong X, Zheng Q.
SETD7 isa prognosis predicting factor of breast cancer and regulates redox homeostasis.
ONCOTARGET . 2017 Oct 6;8(55):94080-94090. (IF 5.168)

119. Dai JP, Wang QW, Su Y, Gu LM, Zhao Y, Chen XX, Chen C, Li WZ, Wang GF, Li KS.
Emodin Inhibition of Influenza A Virus Replication and Influenza Viral Pneumonia via the Nrf2, TLR4, p38/JNK and NF-kappaB Pathways.
Molecules . 2017 Oct 18;22(10). pii: E1754. (IF 3.267)

120. Xie F, Wu D, Huang SF, Cao JG, Li HN, He L, Liu MQ, Li LF, Chen LX.
The endoplasmic reticulum stress-autophagy pathway is involved in apelin-13-inducedcardiomyocyte hypertrophy in vitro.
Acta Pharmacol Sin . 2017 Dec;38(12):1589-1600. (IF 5.064)

121. An B, Lan J, Deng X, Chen S, Ouyang C, Shi H, Yang J, Li Y.
Silencing of D-Lactate Dehydrogenase Impedes Glyoxalase System and Leads to MethylglyoxalAccumulation and Growth Inhibition in Rice.
Front Plant Sci . 2017 Dec 5;8:2071. (IF 4.402)

122. Ren M,Wang T,Huang L,Ye X,Han Z
Mesoporous Silica Nanoparticles Rescue H2O2-induced Inhibition of Cardiac Differentiation.
Cell Struct Funct . 2018;43(2):109-117. (IF 2.429)

123. Liu SY,Lu S,Yu XL,Yang SG,Liu W,Liu XM,Wang SW,Zhu J,Ji M,Liu DQ,Zhang ZP,Liu RT
Fruitless Wolfberry-Sprout Extract Rescued Cognitive Deficits and Attenuated Neuropathology in Alzheimer’s Disease Transgenic Mice.
Curr Alzheimer Res . 2018;15(9):856-868. (IF 3.047)

124. Dai G,Zheng D,Guo W,Yang J,Cheng AY
Cinobufagin Induces Apoptosis in Osteosarcoma Cells Via the Mitochondria-Mediated Apoptotic Pathway.
CELL PHYSIOL BIOCHEM. 2018;46(3):1134-1147. (IF 5.5)

125. You X, Liu L, Li X, Du H, Nie D, Zhang X, Tong H, Wu M, Gao Y, Liao Z.
Immune response ofinterferon-γ-inducible lysosomal thiol reductase (GILT)from Chinesesturgeon (Acipenser sinensis)to microbial invasion andits antioxdative activity in lipopolysaccharides-treated mammalian dentritic cells.
FISH SHELLFISH IMMUN . 2018 Jan;72:356-366. (IF 3.298)

126. Dai J, Gu L, Su Y, Wang Q, Zhao Y, Chen X, Deng H, Li W, Wang G, Li K.
Inhibition of curcumin on influenza A virus infection and influenzal pneumonia via oxidativestress, TLR2/4, p38/JNK MAPK and NF-κB pathways.
Int Immunopharmacol . 2018 Jan;54:177-187. (IF 3.943)

127. Lu MC, Jiao Q, Liu T, Tan SJ, Zhou HS, You QD, Jiang ZY.
Discovery ofa head-to-tail cyclic peptide as the Keap1-Nrf2 protein-protein interaction inhibitor with high cell potency.
Eur J Med Chem . 2018 Jan 1;143:1578-1589. (IF 5.572)

128. Fan XY, Liu YJ, Chen K, Jiang FL, Hu YJ, Liu D, Liu Y, Ge YS.
Organic arsenicals target thioredoxin reductase followed by oxidative stress and mitochondrialdysfunction resulting in apoptosis.
Eur J Med Chem . 2018 Jan 1;143:1090-1102. (IF 5.572)

129. Wang Q, Pu Y, Yang D, Yin X, He Z, Yang Y, Yang Y.
Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea.
BIOCHEM BIOPH RES CO . 2018 Jan 8;495(2):1851-1857. (IF 2.985)

130. Dai J,Gu L,Su Y,Wang Q,Zhao Y,Chen X,Deng H,Li W,Wang G,Li K
Inhibition of curcumin on influenza A virus infection and influenzal pneumonia via oxidative stress, TLR2/4, p38/JNK MAPK and NF-κB pathways.
Int Immunopharmacol . 2018 Jan;54:177-187. (IF 3.943)

131. Lu MC,Jiao Q,Liu T,Tan SJ,Zhou HS,You QD,Jiang ZY
Discovery of a head-to-tail cyclic peptide as the Keap1-Nrf2 protein-protein interaction inhibitor with high cell potency.
Eur J Med Chem . 2018 Jan 1;143:1578-1589. (IF 5.572)

132. Yang X,Wang Z,Li X,Liu B,Liu M,Liu L,Chen S,Ren M,Wang Y,Yu M,Wang B,Zou J,Zhu WG,Yin Y,Gu W,Luo J
SHMT2 Desuccinylation by SIRT5 Drives Cancer Cell Proliferation.
Cancer Res . 2018 Jan 15;78(2):372-386. (IF 9.727)

133. You X,Liu L,Li X,Du H,Nie D,Zhang X,Tong H,Wu M,Gao Y,Liao Z
Immune response of interferon-γ-inducible lysosomal thiol reductase (GILT) from Chinese sturgeon (Acipenser sinensis) to microbial invasion and its antioxdative activity in lipopolysaccharides-treated
FISH SHELLFISH IMMUN. 2018 Jan;72:356-366. (IF 3.298)

134. Fan XY,Liu YJ,Chen K,Jiang FL,Hu YJ,Liu D,Liu Y,Ge YS
Organic arsenicals target thioredoxin reductase followed by oxidative stress and mitochondrial dysfunction resulting in apoptosis.
Eur J Med Chem. 2018 Jan 1;143:1090-1102. (IF 5.572)

135. Zhou Q, Fu X, Wang X, Wu Q, Lu Y, Shi J, Klaunig JE, Zhou S.
Autophagy plays a protective role in Mn-induced toxicity in PC12 cells.
Toxicology . 2018 Feb 1;394:45-53. (IF 4.099)

136. Zhang Y, Li XR, Zhao L, Duan GL, Xiao L, Chen HP.
DJ-1 preserving mitochondrial complex I activity plays a critical role in resveratrol-mediatedcardioprotection against hypoxia/reoxygenation-induced oxidative stress.
Biomed Pharmacother . 2018 Feb;98:545-552. (IF 4.545)

137. Fu LH, Wei ZZ, Hu KD, Hu LY, Li YH, Chen XY, Han Z, Yao GF, Zhang H.
Hydrogen sulfide inhibits the growth of Escherichia coli through oxidative damage.
J Microbiol . 2018 Feb 28. (IF 2.845)

138. Liu Y, Xu C, Zhu Y, Zhang L, Chen T, Zhou F, Chen H, Lin Y.
The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice.
J Integr Plant Biol . 2018 Feb;60(2):173-188. (IF 4.885)

139. Su YQ, Zhao YJ, Wu N, Chen YE, Zhang WJ, Qiao DR, Cao Y.
Chromium removal from solution by five photosynthetic bacteria isolates.
APPL MICROBIOL BIOT . 2018 Feb;102(4):1983-1995. (IF 3.53)

140. Huang D,Yin L,Liu X,Lv B,Xie Z,Wang X,Yu B,Zhang Y
Geraniin protects bone marrow‑derived mesenchymal stem cells against hydrogen peroxide‑induced cellular oxidative stress in vitro.
Int J Mol Med . 2018 Feb;41(2):739-748. (IF 3.098)

141. Ma J,Song D,Zhang Y,Chen L,Zhang S,Jia J,Chen T,Guo C,Tian L,Gao A,Niu P
SIRT1 exhibits antioxidative effects in HT22 cells induced by tert-butyl alcohol.
Environ Toxicol . 2018 Feb;33(2):142-148. (IF 3.118)

142. Li B,He X,Zhuang M,Niu B,Wu C,Mu H,Tang F,Cui Y,Liu W,Zhao B,Peng S,Li G,Hua J
Melatonin Ameliorates Busulfan-Induced Spermatogonial Stem Cell Oxidative Apoptosis in Mouse Testes.
ANTIOXID REDOX SIGN . 2018 Feb 10;28(5):385-400. (IF 6.323)

143. Zhang Y,Li XR,Zhao L,Duan GL,Xiao L,Chen HP
DJ-1 preserving mitochondrial complex I activity plays a critical role in resveratrol-mediated cardioprotection against hypoxia/reoxygenation-induced oxidative stress.
Biomed Pharmacother. 2018 Feb;98:545-552. (IF 4.545)

144. Luo CQ, Zhou YX, Zhou TJ, Xing L, Cui PF, Sun M, Jin L, Lu N, Jiang HL.
Reactive oxygen species-responsive nanoprodrug with quinone methides-mediated GSHdepletion for improved chlorambucil breast cancers therapy.
J Control Release . 2018 Mar 28;274:56-68. (IF 7.727)

145. Zhang S, An Q, Wang T, Gao S, Zhou G.
Autophagy-and MMP-2/9-mediated Reduction and Redistribution of ZO-1 Contribute to Hyperglycemia-increased Blood-Brain Barrier Permeability during Early Reperfusion in Stroke.
Neuroscience . 2018 Mar 7. pii: S0306-4522(18)30160-X. (IF 3.056)

146. Bai Z, Gao M, Xu X, Zhang H, Xu J, Guan Q, Wang Q, Du J, Li Z, Zuo D, Zhang W, Wu Y.
Overcoming resistance to mitochondrial apoptosis by BZML-induced mitotic catastrophe is enhanced by inhibition of autophagy in A549/Taxol cells.
CELL PROLIFERAT . 2018 Mar 1. (IF 5.753)

147. Wang J,Zhang J,Chen X,Yang Y,Wang F,Li W,Awuti M,Sun Y,Lian C,Li Z,Wang M,Xu JY,Jin C,Tian H,Gao F,Zhang J,Sinha D,Lu L,Xu GT
miR-365 promotes diabetic retinopathy through inhibiting Timp3 and increasing oxidative stress.
Exp Eye Res . 2018 Mar;168:89-99. (IF 3.011)

148. Chen J,Zhang DM,Feng X,Wang J,Qin YY,Zhang T,Huang Q,Sheng R,Chen Z,Li M,Qin ZH
TIGAR inhibits ischemia/reperfusion-induced inflammatory response of astrocytes.
Neuropharmacology . 2018 Mar 15;131:377-388. (IF 4.431)

149. Qiu M,Shi F,Dai F,Song R,Wang S,You Y,Zhao B
A reactive oxygen species activation mechanism contributes to Sophoridine-induced apoptosis in rat liver BRL-3A cells.
J Ethnopharmacol. 2018 Mar 1;213:376-383. (IF 3.69)

150. Luo CQ,Zhou YX,Zhou TJ,Xing L,Cui PF,Sun M,Jin L,Lu N,Jiang HL
Reactive oxygen species-responsive nanoprodrug with quinone methides-mediated GSH depletion for improved chlorambucil breast cancers therapy.
J Control Release. 2018 Mar 28;274:56-68. (IF 7.727)

151. Guo J,Xu B,Han Q,Zhou H,Xia Y,Gong C,Dai X,Li Z,Wu G
Ferroptosis: A Novel Anti-tumor Action for Cisplatin.
Cancer Res Treat . 2018 Apr;50(2):445-460. (IF 3.761)

152. Lei Q,Tan J,Yi S,Wu N,Wang Y,Wu H
Mitochonic acid 5 activates the MAPK-ERK-yap signaling pathways to protect mouse microglial BV-2 cells against TNFα-induced apoptosis via increased Bnip3-related mitophagy.
Cell Mol Biol Lett . 2018 Apr 5;23:14. (IF 3.451)

153. Gui L,Zhou J,Zhou L,Wei S
A smart copper-phthalocyanine framework nanoparticle for enhancing photodynamic therapy in hypoxic conditions by weakening cells through ATP depletion.
J Mater Chem B. 2018 Apr 14;6(14):2078-2088. (IF 5.344)

154. Wang J,Zhang Y
Neuroprotective effect of berberine agonist against impairment of learning and memory skills in severe traumatic brain injury via Sirt1/p38 MAPK expression.
Mol Med Rep . 2018 May;17(5):6881-6886. (IF 2.1)

155. Wu D,Han R,Deng S,Liu T,Zhang T,Xie H,Xu Y
Protective Effects of Flagellin A N/C Against Radiation-Induced NLR Pyrin Domain Containing 3 Inflammasome-Dependent Pyroptosis in Intestinal Cells.
INT J RADIAT ONCOL . 2018 May 1;101(1):107-117. (IF 5.859)

156. Wang W,Zheng W,Hu F,He X,Wu D,Zhang W,Liu H,Ma X
Enhanced Biosynthesis Performance of Heterologous Proteins in CHO-K1 Cells Using CRISPR-Cas9.
ACS Synth Biol. 2018 May 18;7(5):1259-1268. (IF 4.411)

157. Zhang S, An Q, Wang T, Gao S, Zhou G.
Autophagy- and MMP-2/9-mediated Reduction and Redistribution of ZO-1 Contribute to Hyperglycemia-increased Blood-Brain Barrier Permeability During Early Reperfusion in Stroke.
Neuroscience. 2018 May 1;377:126-137. (IF 3.056)

158. Feng M, Yin H, Cao Y, Peng H, Lu G, Liu Z, Dang Z.
Cadmium-induced stress response of Phanerochaete chrysosporium duringthe biodegradation of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47).
ECOTOX ENVIRON SAFE . 2018 Jun 15;154:45-51. (IF 4.872)

159. Wang H,Shen X,Tian G,Shi X,Huang W,Wu Y,Sun L,Peng C,Liu S,Huang Y,Chen X,Zhang F,Chen Y,Ding W,Lu Z
AMPKα2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction.
FREE RADICAL BIO MED . 2018 Jun;121:202-214. (IF 6.17)

160. Sun X,Wang P,Yao LP,Wang W,Gao YM,Zhang J,Fu YJ
Paeonol alleviated acute alcohol-induced liver injury via SIRT1/Nrf2/NF-κB signaling pathway.
ENVIRON TOXICOL CHEM. 2018 Jun;60:110-117. (IF 3.152)

161. Feng M,Yin H,Cao Y,Peng H,Lu G,Liu Z,Dang Z
Cadmium-induced stress response of Phanerochaete chrysosporium during the biodegradation of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47).
ECOTOX ENVIRON SAFE. 2018 Jun 15;154:45-51. (IF 4.872)

162. Wang Y,Lei T,Yuan J,Wu Y,Shen X,Gao J,Feng W,Lu Z
GCN2 deficiency ameliorates doxorubicin-induced cardiotoxicity by decreasing cardiomyocyte apoptosis and myocardial oxidative stress.
Redox Biol . 2018 Jul;17:25-34. (IF 9.986)

163. Tang Q,Bai L,Zou Z,Meng P,Xia Y,Cheng S,Mu S,Zhou J,Wang X,Qin X,Cao X,Jiang X,Chen C
Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure.
Neurotoxicology. 2018 Jul;67:27-36. (IF 3.105)

164. Wang Z,Zhang J,Wang L,Li W,Chen L,Li J,Zhao D,Zhang H,Guo X
Glycine mitigates renal oxidative stress by suppressing Nox4 expression in rats with streptozotocin-induced diabetes.
J Pharmacol Sci . 2018 Aug;137(4):387-394. (IF 2.835)

165. Ma X,Hu B,Zou C,Han A,Xu Z,Zhang T,Yu W
The effects of hyperoxia liquid regulate cardiopulmonary bypass‑induced myocardial damage through the Nrf2‑ARE signaling pathway.
Mol Med Rep . 2018 Aug;18(2):2342-2348. (IF 2.1)

166. Xu C,Sun Y,Qi Y,Yu Y,He Y,Hu M,Hu Q,Wu T,Zhang D,Shang L,Deng H,Zhang Z
Selective self-induced stimulus amplification prodrug platform for inhibiting multidrug resistance and lung metastasis.
J Control Release. 2018 Aug 28;284:224-239. (IF 7.727)

167. Li K,Yang K,Zheng L,Li Y,Wang Q,Lin R,He D
Anti-acute myeloid leukemia activity of 2-chloro-3-alkyl-1,4-naphthoquinone derivatives through inducing mtDNA damage and GSH depletion.
BIOORG MED CHEM LETT. 2018 Aug 7;26(14):4191-4200. (IF 2.572)

168. Li F,Li X,Cui P
Detoxification of U(VI) by Paecilomyces catenlannulatus investigated by batch, XANES and EXAFS techniques.
J Environ Radioact . 2018 Sep;189:24-30.

169. Xie HL,Zhu S,Zhang J,Wen J,Yuan HJ,Pan LZ,Luo MJ,Tan JH
Glucose metabolism during in vitro maturation of mouse oocytes: An study using RNA interference.
J Cell Physiol . 2018 Sep;233(9):6952-6964. (IF 5.546)

170. Feng RB,Wang Y,He C,Yang Y,Wan JB
Gallic acid, a natural polyphenol, protects against tert-butyl hydroperoxide- induced hepatotoxicity by activating ERK-Nrf2-Keap1-mediated antioxidative response.
Food Chem Toxicol . 2018 Sep;119:479-488. (IF 4.679)

171. Wang D,Xie N,Gao W,Kang R,Tang D
The ferroptosis inducer erastin promotes proliferation and differentiation in human peripheral blood mononuclear cells.
BIOCHEM BIOPH RES CO . 2018 Sep 10;503(3):1689-1695. (IF 2.985)

172. Bao J,Ye C,Zheng Z,Zhou Z
Fmr1 protects cardiomyocytes against lipopolysaccharide-induced myocardial injury.
Exp Ther Med. 2018 Sep;16(3):1825-1833. (IF 1.785)

173. Ding A,Wen X
Dandelion root extract protects NCM460 colonic cells and relieves experimental mouse colitis.
J NAT MED-TOKYO. 2018 Sep;72(4):857-866. (IF 2.055)

174. Wang Q,Bu S,Xin D,Li B,Wang L,Lai D
Autophagy Is Indispensable for the Self-Renewal and Quiescence of Ovarian Cancer Spheroid Cells with Stem Cell-Like Properties.
Oxid Med Cell Longev. 2018 Sep 17;2018:7010472. (IF 5.076)

175. Xu KY,Wu CY,Tong S,Xiong P,Wang SH
The selective Nlrp3 inflammasome inhibitor Mcc950 attenuates lung ischemia-reperfusion injury.
BIOCHEM BIOPH RES CO. 2018 Sep 18;503(4):3031-3037. (IF 2.985)

176. Yue L,Dai Z,Chen X,Liu C,Hu Z,Song B,Zheng X
Development of a novel FePt-based multifunctional ferroptosis agent for high-efficiency anticancer therapy.
Nanoscale. 2018 Sep 27;10(37):17858-17864. (IF 6.895)

177. Wang XX,Zhang TY,Dao GH,Hu HY
Tolerance and resistance characteristics of microalgae Scenedesmus sp. LX1 to methylisothiazolinone.
Environ Pollut . 2018 Oct;241:200-211. (IF 6.792)

178. Liu S,Yuan J,Yue W,Bi Y,Shen X,Gao J,Xu X,Lu Z
GCN2 deficiency protects against high fat diet induced hepatic steatosis and insulin resistance in mice.
BBA-MOL BASIS DIS . 2018 Oct;1864(10):3257-3267. (IF 4.352)

179. Middepogu A,Hou J,Gao X,Lin D
Effect and mechanism of TiO2 nanoparticles on the photosynthesis of Chlorella pyrenoidosa.
ECOTOX ENVIRON SAFE. 2018 Oct;161:497-506. (IF 4.872)

180. Qiu YL,Cheng XN,Bai F,Fang LY,Hu HZ,Sun DQ
Aucubin protects against lipopolysaccharide-induced acute pulmonary injury through regulating Nrf2 and AMPK pathways.
Biomed Pharmacother. 2018 Oct;106:192-199. (IF 4.545)

181. Wang Z,Luo H,Xia H
Theaflavins attenuate ethanol‑induced oxidative stress and cell apoptosis in gastric mucosa epithelial cells via downregulation of the mitogen‑activated protein kinase pathway.
Mol Med Rep. 2018 Oct;18(4):3791-3799. (IF 2.1)

182. Wang KF,Guo C,Ju F,Samak NA,Zhuang GQ,Liu CZ
Farnesol-induced hyperbranched morphology with short hyphae and bulbous tips of Coriolus versicolor.
SCI REP-UK. 2018 Oct 12;8(1):15213. (IF 3.998)

183. He H,Qiao Y,Zhang Z,Wu Z,Liu D,Liao Z,Yin D,He M
Dual action of vitamin C in iron supplement therapeutics for iron deficiency anemia: prevention of liver damage induced by iron overload.
Food Funct. 2018 Oct 17;9(10):5390-5401. (IF 4.171)

184. Liang Y,Huang W,Zeng D,Huang X,Chan L,Mei C,Feng P,Tan CH,Chen T
Cancer-targeted design of bioresponsive prodrug with enhanced cellular uptake to achieve precise cancer therapy.
Drug Deliv . 2018 Nov;25(1):1350-1361. (IF 4.902)

185. Xie Z,Guo Z,Wang Y,Lei J,Yu J
Protocatechuic acid inhibits the growth of ovarian cancer cells by inducing apoptosis and autophagy.
Phytother Res. 2018 Nov;32(11):2256-2263. (IF 4.087)

186. Li H,Li D,He Z,Fan J,Li Q,Liu X,Guo P,Zhang H,Chen S,Li Q,Zhang R,Xing X,Zeng X,Zhang A,Tang H,Dong G,Wang Q,Xiao Y,Chen L,Chen W
The effects of Nrf2 knockout on regulation of benzene-induced mouse hematotoxicity.
TOXICOL APPL PHARM . 2018 Nov 1;358:56-67. (IF 3.616)

187. Yang Y,Wang G,Wu W,Yao S,Han X,He D,He J,Zheng G,Zhao Y,Cai Z,Yu R
Camalexin Induces Apoptosis via the ROS-ER Stress-Mitochondrial Apoptosis Pathway in AML Cells.
Oxid Med Cell Longev. 2018 Nov 14;2018:7426950. (IF 5.076)

188. Lei L,Hu H,Lei Y,Feng J
Leukocytic toll-like receptor 2 knockout protects against diabetes-induced cardiac dysfunction.
BIOCHEM BIOPH RES CO. 2018 Nov 30;506(3):668-673. (IF 2.985)

189. Liang S,Han L,Mu W,Jiang D,Hou T,Yin X,Pang X,Yang R,Liu Y,Zhang N
Carboplatin-loaded SMNDs to reduce GSH-mediated platinum resistance for prostate cancer therapy.
J Mater Chem B. 2018 Nov 21;6(43):7004-7014. (IF 5.344)

190. Wang W,Wang X,Zhang XS,Liang CZ
Cryptotanshinone Attenuates Oxidative Stress and Inflammation through the Regulation of Nrf-2 and NF-κB in Mice with Unilateral Ureteral Obstruction.
BASIC CLIN PHARMACOL . 2018 Dec;123(6):714-720. (IF 2.651)

191. Xu S,Zhao X,Liu F,Cao Y,Wang B,Wang X,Yin M,Wang Q,Feng X
Crucial role of oxidative stress in bactericidal effect of parthenolide against Xanthomonas oryzae pv. oryzae.
Pest Manag Sci . 2018 Dec;74(12):2716-2723. (IF 3.75)

192. Zhang X,Högy P,Wu X,Schmid I,Wang X,Schulze WX,Jiang D,Fangmeier A
Physiological and Proteomic Evidence for the Interactive Effects of Post-Anthesis Heat Stress and Elevated CO2 on Wheat.
Proteomics . 2018 Dec;18(23):e1800262. (IF 3.254)

193. Wang XX,Zhang TY,Dao GH,Hu HY
Interaction between 1,2-benzisothiazol-3(2H)-one and microalgae: Growth inhibition and detoxification mechanism.
Aquat Toxicol . 2018 Dec;205:66-75. (IF 4.344)

194. Liu SY,Song JY,Fan B,Wang Y,Pan YR,Che L,Sun YJ,Li GY
Resveratrol protects photoreceptors by blocking caspase- and PARP-dependent cell death pathways.
FREE RADICAL BIO MED. 2018 Dec;129:569-581. (IF 6.17)

195. Meng Q,Xu J,Liang C,Liu J,Hua J,Zhang Y,Ni Q,Shi S,Yu X
GPx1 is involved in the induction of protective autophagy in pancreatic cancer cells in response to glucose deprivation.
Cell Death Dis. 2018 Dec 11;9(12):1187. (IF 6.304)

196. Wu X,Li X,Li Z,Yu Y,You Q,Zhang X
Discovery of Nonquinone Substrates for NAD(P)H: Quinone Oxidoreductase 1 (NQO1) as Effective Intracellular ROS Generators for the Treatment of Drug-Resistant Non-Small-Cell Lung Cancer.
J Med Chem. 2018 Dec 27;61(24):11280-11297. (IF 6.205)

197. Ren C,Wu H,Li D,Yang Y,Gao Y,Jizhang Y,Liu D,Ji X,Zhang X
Remote Ischemic Conditioning Protects Diabetic Retinopathy in Streptozotocin-induced Diabetic Rats via Anti-Inflammation and Antioxidation.
Aging Dis. 2018 Dec 4;9(6):1122-1133. (IF 5.402)

198. Qiu M,Zhang S,Ke L,Tang H,Zeng X,Liu J
JS-K enhances chemosensitivity of prostate cancer cells to Taxol via reactive oxygen species activation.
Oncol Lett. 2019 Jan;17(1):757-764. (IF 2.311)

199. Zhang J,Wang B,Zhao B,Li Y,Zhao X,Yuan Z
Blueberry anthocyanin alleviate perfluorooctanoic acid-induced toxicity in planarian (Dugesia japonica) by regulating oxidative stress biomarkers, ATP contents, DNA methylation and mRNA expression.
Environ Pollut. 2019 Feb;245:957-964. (IF 6.792)

200. Shi F, Pan H, Cui B, Li Y, Huang L, Lu Y.
Dictamnine-induced hepatotoxicity in mice: the role of metabolic activation of furan.
TOXICOL APPL PHARM . 2019 Feb 1;364:68-76. (IF 3.616)

201. Hu X,Liang Y,Zhao B,Wang Y
Thymoquinone protects human retinal pigment epithelial cells against hydrogen peroxide induced oxidative stress and apoptosis.
J Cell Biochem. 2019 Mar;120(3):4514-4522. (IF 4.237)

202. Liu Z,Li T,Zhu F,Deng S,Li X,He Y
Regulatory roles of miR-22/Redd1-mediated mitochondrial ROS and cellular autophagy in ionizing radiation-induced BMSC injury.
Cell Death Dis. 2019 Mar 7;10(3):227. (IF 6.304)

203. Wang SQ,Yang XY,Cui SX,Gao ZH,Qu XJ
Heterozygous knockout insulin-like growth factor-1 receptor (IGF-1R) regulates mitochondrial functions and prevents colitis and colorectal cancer.
FREE RADICAL BIO MED. 2019 Apr;134:87-98. (IF 6.17)

204. Zhao MX,Wen JL,Wang L,Wang XP,Chen TS
Intracellular catalase activity instead of glutathione level dominates the resistance of cells to reactive oxygen species.
CELL STRESS CHAPERON. 2019 May;24(3):609-619. (IF 2.892)

205. Xu L,Wang L,Zhou L,Dorfman RG,Pan Y,Tang D,Wang Y,Yin Y,Jiang C,Zou X,Wu J,Zhang M
The SIRT2/cMYC Pathway Inhibits Peroxidation-Related Apoptosis In Cholangiocarcinoma Through Metabolic Reprogramming.
Neoplasia. 2019 May;21(5):429-441. (IF 5.696)

206. Bai F,Yu Z,Gao X,Gong J,Fan L,Liu F
Simvastatin induces breast cancer cell death through oxidative stress up-regulating miR-140-5p.
AGING-US. 2019 May 28;11(10):3198-3219. (IF 4.831)

207. Huang C,Wen C,Yang M,Gan D,Fan C,Li A,Li Q,Zhao J,Zhu L,Lu D
Lycopene protects against t-BHP-induced neuronal oxidative damage and apoptosis via activation of the PI3K/Akt pathway.
Mol Biol Rep. 2019 Jun;46(3):3387-3397. (IF 1.402)

208. Xu C,Xu J,Ji G,Liu Q,Shao W,Chen Y,Gu J,Weng Z,Zhang X,Wang Y,Gu A
Deficiency of X-ray repair cross-complementing group 1 in primordial germ cells contributes to male infertility.
FASEB J. 2019 Jun;33(6):7427-7436. (IF 4.966)

209. Mao M,Zhang T,Wang Z,Wang H,Xu J,Yin F,Wang G,Sun M,Wang Z,Hua Y,Cai Z
Glaucocalyxin A-induced oxidative stress inhibits the activation of STAT3 signaling pathway and suppresses osteosarcoma progression in vitro and in vivo.
BBA-MOL BASIS DIS. 2019 Jun 1;1865(6):1214-1225. (IF 4.352)

210. Wei Y,Wang R,Teng J
Inhibition of Calcium/Calmodulin-Dependent Protein Kinase IIα Suppresses Oxidative Stress in Cerebral Ischemic Rats Through Targeting Glucose 6-Phosphate Dehydrogenase.
Neurochem Res. 2019 Jul;44(7):1613-1620. (IF 3.038)

211. Li HS,Zhou YN,Li L,Li SF,Long D,Chen XL,Zhang JB,Feng L,Li YP
HIF-1α protects against oxidative stress by directly targeting mitochondria.
Redox Biol. 2019 Jul;25:101109. (IF 9.986)

212. Li R,Liu Y,Shan YG,Gao L,Wang F,Qiu CG
Bailcalin Protects against Diabetic Cardiomyopathy through Keap1/Nrf2/AMPK-Mediated Antioxidative and Lipid-Lowering Effects.
Oxid Med Cell Longev. 2019 Jul 1;2019:3206542. (IF 5.076)

213. Chen Y,Zheng Q,Jia X,Chen K,Wang Y,Wu T,Xu X,Han Z,Zhang Z,Zhang X
MdGGT1 Impacts Apple miR156 Precursor Levels via Ontogenetic Changes in Subcellular Glutathione Homeostasis.
Front Plant Sci. 2019 Jul 31;10:994. (IF 4.402)

214. Luca M,Di Mauro M,Perry G
Neuropsychiatric Disturbances and Diabetes Mellitus: The Role of Oxidative Stress.
Oxid Med Cell Longev. 2019 Jul 4;2019:5698132. (IF 5.076)

215. Lu MC,Zhao J,Liu YT,Liu T,Tao MM,You QD,Jiang ZY
CPUY192018, a potent inhibitor of the Keap1-Nrf2 protein-protein interaction, alleviates renal inflammation in mice by restricting oxidative stress and NF-κB activation.
Redox Biol. 2019 Sep;26:101266. (IF 9.986)

216. Zeng R,Wang X,Zhou Q,Fu X,Wu Q,Lu Y,Shi J,Klaunig JE,Zhou S
Icariin protects rotenone-induced neurotoxicity through induction of SIRT3.
TOXICOL APPL PHARM . 2019 Sep 15;379:114639. (IF 3.616)

217. Xiao D,Yuan D,Tan B,Wang J,Liu Y,Tan B
The Role of Nrf2 Signaling Pathway in Eucommia ulmoides Flavones Regulating Oxidative Stress in the Intestine of Piglets.
Oxid Med Cell Longev. 2019 Sep 2;2019:9719618. (IF 5.076)

218. Zhang J,Sheng J,Dong L,Xu Y,Yu L,Liu Y,Huang X,Wan S,Lan HY,Wang H
Cardiomyocyte-specific loss of RNA polymerase II subunit 5-mediating protein causes myocardial dysfunction and heart failure.
Cardiovasc Res. 2019 Sep 1;115(11):1617-1628. (IF 8.168)

219. Yu Z,Yu Z,Chen Z,Yang L,Ma M,Lu S,Wang C,Teng C,Nie Y
Zinc chelator TPEN induces pancreatic cancer cell death through causing oxidative stress and inhibiting cell autophagy.
J Cell Physiol. 2019 Nov;234(11):20648-20661. (IF 5.546)

220. Xiao Z,Liang P,Chen J,Chen MF,Gong F,Li C,Zhou C,Hong P,Yang P,Qian ZJ
A Peptide YGDEY from Tilapia Gelatin Hydrolysates Inhibits UVB-mediated Skin Photoaging by Regulating MMP-1 and MMP-9 Expression in HaCaT Cells.
Photochem Photobiol. 2019 Nov;95(6):1424-1432. (IF 2.721)

221. Huang L,Cai C,Dang W,Lu JH,Hu GF,Gu J
Propyl isothiocyanate induces apoptosis in gastric cancer cells by oxidative stress via glutathione depletion.
Oncol Lett. 2019 Nov;18(5):5490-5498. (IF 2.311)

222. Li K,Dong W,Qiu L,Liu Q,Lv G,Peng Y,Xie M,Lin J
A new GSH-responsive prodrug of 5-aminolevulinic acid for photodiagnosis and photodynamic therapy of tumors.
Eur J Med Chem. 2019 Nov 1;181:111582. (IF 5.572)

223. Hu X,Liu H,Wang Z,Hu Z,Li L
miR-200a Attenuated Doxorubicin-Induced Cardiotoxicity through Upregulation of Nrf2 in Mice.
Oxid Med Cell Longev. 2019 Nov 3;2019:1512326. (IF 5.076)

224. Guo M,Zhou X,Han X,Zhang Y,Jiang L
SPINK1 is a prognosis predicting factor of non-small cell lung cancer and regulates redox homeostasis.
Oncol Lett. 2019 Dec;18(6):6899-6908. (IF 2.311)

225. Guo H,Xu J,Zheng Q,He J,Zhou W,Wang K,Huang X,Fan Q,Ma J,Cheng J,Mei W,Xing R,Cai R
NRF2 SUMOylation promotes de novo serine synthesis and maintains HCC tumorigenesis.
Cancer Lett. 2019 Dec 1;466:39-48. (IF 7.36)

226. Guo J,Zhang T,Gu J,Cai K,Deng X,Chen K,Huang K,Wang G,Li H,Wang J
Oleic Acid Protects against Hepatic Ischemia and Reperfusion Injury in Mice by Inhibiting AKT/mTOR Pathways.
Oxid Med Cell Longev. 2019 Dec 13;2019:4842592. (IF 5.076)

227. Zhou Q,Wang X,Shao X,Wang H,Liu X,Ke X,Xiong C,Wei L,Zou H
tert-Butylhydroquinone Treatment Alleviates Contrast-Induced Nephropathy in Rats by Activating the Nrf2/Sirt3/SOD2 Signaling Pathway.
Oxid Med Cell Longev. 2019 Dec 18;2019:4657651. (IF 5.076)

228. Xin Li,Kejing Zhang,Yu Hu,Na Luo
ERRα activates SHMT2 transcription to enhance the resistance of breast cancer to lapatinib via modulating the mitochondrial metabolic adaption
Biosci Rep. 2020 Jan 31;40(1):BSR20192465.;doi: 10.1042/BSR20192465.

229. Man Luo,Ji-Cheng Huang,Zhan-Qing Yang,Yu-Si Wang,Bin Guo,Zhan-Peng Yue
Hydroxysafflor Yellow A Exerts Beneficial Effects by Restoring Hormone Secretion and Alleviating Oxidative Stress in Polycystic Ovary Syndrome Mice
Exp Physiol. 2020 Feb;105(2):282-292.;doi: 10.1113/EP088147. (IF 2.431)

230. Yuanyuan Zhou,Xiaoya Xu,Jie Wu,Lingling Xu,Min Zhang,Zegeng Li,Dianlei Wang
Allyl isothiocyanate treatment alleviates chronic obstructive pulmonary disease through the Nrf2-Notch1 signaling and upregulation of MRP1
Life Sci. 2020 Feb 15;243:117291.;doi: 10.1016/j.lfs.2020.117291.

231. Hai Wang,Can Liu,Yongxin Zhao,Wenling Zhang,Keqian Xu,Dai Li,Yong Zhou,Hao Li,Guishan Xiao,Bin Lu,Ge Gao
Inhibition of LONP1 protects against erastin-induced ferroptosis in Pancreatic ductal adenocarcinoma PANC1 cells
Biochem Biophys Res Commun. 2020 Feb 19;522(4):1063-1068.;doi: 10.1016/j.bbrc.2019.11.187.

232. Man Luo,Zhan-Qing Yang,Ji-Cheng Huang,Yu-Si Wang,Bin Guo,Zhan-Peng Yue
Genistein protects ovarian granulosa cells from oxidative stress via cAMP-PKA signaling
Cell Biol Int. 2020 Feb;44(2):433-445.;doi: 10.1002/cbin.11244.

233. Wu T,He K,Liang X,Wei T,Wang Y,Zou L,Zhang T,Xue Y,Tang M
The glycolytic shift was involved in CdTe/ZnS quantum dots inducing microglial activation mediated through the mTOR signaling pathway.
J Appl Toxicol. 2020 Mar;40(3):388-402. (IF 2.997)

234. Hai-Fan Yu,Cui-Cui Duan,Zhan-Qing Yang,Yu-Si Wang,Zhan-Peng Yue,Bin Guo
Malic enzyme 1 is important for uterine decidualization in response to progesterone/cAMP/PKA/HB-EGF pathway
FASEB J. 2020 Mar;34(3):3820-3837.;doi: 10.1096/fj.201902289R.

235. De-Ming Liu,Dong Yang,Chun-Yan Zhou,Jia-Si Wu,Guo-Lin Zhang,Ping Wang,Fei Wang,Xian-Li Meng
Aloe-emodin induces hepatotoxicity by the inhibition of multidrug resistance protein 2
Phytomedicine. 2020 Mar;68:153148.;doi: 10.1016/j.phymed.2019.153148.

236. Jiang H,Li R,Zhang Z,Chang C,Liu Y,Liu Z,He Q,Wang Q
Retinoid X receptor α (RXRα)-mediated erythroid-2-related factor-2 (NRF2) inactivation contributes to N,N-dimethylformamide (DMF)-induced oxidative stress in HL-7702 and HuH6 cells.
J Appl Toxicol. 2020 Apr;40(4):470-482. (IF 2.997)

237. Yan Chen,Ning Li,Haijing Wang,Ningning Wang,Hui Peng,Jing Wang,Yihong Li,Mingdi Liu,Hui Li,Yu Zhang,Zhaohui Wang
Amentoflavone suppresses cell proliferation and induces cell death through triggering autophagy-dependent ferroptosis in human glioma
Life Sci. 2020 Apr 15;247:117425.;doi: 10.1016/j.lfs.2020.117425.

238. Xuwei Liu,Kexin Li,Fengmei Zhang,Yunfei Zhang,Chun Deng,Chunbao Guo
Ablation of glutaredoxin 1 promotes pulmonary angiogenesis and alveolar formation in hyperoxia-injured lungs by modifying HIF-1α stability and inhibiting the NF-κB pathway
Biochem Biophys Res Commun. 2020 Apr 30;525(2):528-535.;doi: 10.1016/j.bbrc.2020.02.129.

239. Yu Wu,Yu-Gang Shi,Xiao-Liang Zheng,Ya-Li Dang,Chen-Min Zhu,Run-Run Zhang,Yu-Ying Fu,Tian-Yi Zhou,Jun-Hui Li
Lipophilic ferulic acid derivatives protect PC12 cells against oxidative damage via modulating β-amyloid aggregation and activating Nrf2 enzymes
Food Funct. 2020 May 1;11(5):4707-4718.;doi: 10.1039/d0fo00800a.

240. Wenjin Guo,Juxiong Liu,Jingxuan Sun,Qian Gong,He Ma,Xingchi Kan,Yu Cao,Jianfa Wang,Shoupeng Fu
Butyrate alleviates oxidative stress by regulating NRF2 nuclear accumulation and H3K9/14 acetylation via GPR109A in bovine mammary epithelial cells and mammary glands
Free Radic Biol Med. 2020 May 20;152:728-742.;doi: 10.1016/j.freeradbiomed.2020.01.016.

241. Yunwen Yang,Suwen Liu,Huiping Gao,Peipei Wang,Yue Zhang,Aihua Zhang,Zhanjun Jia,Songming Huang
Ursodeoxycholic acid protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction through acting on ALDH1L2
Free Radic Biol Med. 2020 May 20;152:821-837.;doi: 10.1016/j.freeradbiomed.2020.01.182.

242. Qihui Jin,Yanhong Jiang,Lizhong Fu,Yanqiu Zheng,Yuxia Ding,Qian Liu
Wenxin Granule Ameliorates Hypoxia/Reoxygenation-Induced Oxidative Stress in Mitochondria via the PKC- δ/NOX2/ROS Pathway in H9c2 Cells
Oxid Med Cell Longev. 2020 May 20;2020:3245483.;doi: 10.1155/2020/3245483.

243. Hui He,Zhen Zou,Bin Wang,Ge Xu,Chengzhi Chen,Xia Qin,Chao Yu,Jun Zhang
Copper Oxide Nanoparticles Induce Oxidative DNA Damage and Cell Death via Copper Ion-Mediated P38 MAPK Activation in Vascular Endothelial Cells
Int J Nanomedicine. 2020 May 8;15:3291-3302.;doi: 10.2147/IJN.S241157.

244. Jiani Xie,Chengyan Wang,Ning Wang,Shuang Zhu,Linqiang Mei,Xiao Zhang,Yuan Yong,Lele Li,Chunying Chen,Changshui Huang,Zhanjun Gu,Yuliang Li,Yuliang Zhao
Graphdiyne nanoradioprotector with efficient free radical scavenging ability for mitigating radiation-induced gastrointestinal tract damage
Biomaterials. 2020 Jun;244:119940.;doi: 10.1016/j.biomaterials.2020.119940.

245. Wang Zhuoya,Zhao Nannan,Zhang Aiping,Wang Guoyan,Dong Menghua,Zhang Jiashen,Xiong Yanlian,Luan Xiying
Human placenta derived mesenchymal stromal cells alleviate GVHD by promoting the generation of GSH and GST in PD-1 + T cells
Cell Immunol. 2020 Jun;352:104083.;doi: 10.1016/j.cellimm.2020.104083.

246. Jun Chu,Bo Yan,Jie Zhang,Lilan Peng,Xiang Ao,Zhenyu Zheng,Tao Jiang,Zhongmin Zhang
Casticin Attenuates Osteoarthritis-Related Cartilage Degeneration by Inhibiting the ROS-Mediated NF-κB Signaling Pathway in vitro and in vivo
Inflammation. 2020 Jun;43(3):810-820.;doi: 10.1007/s10753-019-01167-y.

247. Hui He,Shiquan Xiao,Ge Xu,Bin Wang,Zhen Zou,Xia Qin,Chao Yu,Jun Zhang
The NADPH oxidase 4 protects vascular endothelial cells from copper oxide nanoparticles-induced oxidative stress and cell death
Life Sci. 2020 Jul 1;252:117571.;doi: 10.1016/j.lfs.2020.117571.

248. Jun Luo,Li Li,Die Hu,Xian Zhang
LINC00612/miR-31-5p/Notch1 Axis Regulates Apoptosis, Inflammation, and Oxidative Stress in Human Pulmonary Microvascular Endothelial Cells Induced by Cigarette Smoke Extract
Int J Chron Obstruct Pulmon Dis. 2020 Aug 26;15:2049-2060.;doi: 10.2147/COPD.S255696.

249. Jinhui Hu,Ting Wang,Lin Zhou,Shaohua Wei
A ROS responsive nanomedicine with enhanced photodynamic therapy via dual mechanisms: GSH depletion and biosynthesis inhibition
J Photochem Photobiol B. 2020 Aug;209:111955.;doi: 10.1016/j.jphotobiol.2020.111955.

250. Qi Lv,Haiyue Niu,Lanzhu Yue,Jiaxi Liu,Liyan Yang,Chunyan Liu,Huijuan Jiang,Shuwen Dong,Zonghong Shao,Limin Xing,Huaquan Wang
Abnormal Ferroptosis in Myelodysplastic Syndrome
Front Oncol. 2020 Sep 2;10:1656.;doi: 10.3389/fonc.2020.01656.

251. Rui Zhang,Jie Shi,Tingting Wang,Xiaonan Qiu,Ruixia Liu,Yitian Li,Qing Gao,Ning Wang
Apigetrin ameliorates streptozotocin-induced pancreatic β-cell damages via attenuating endoplasmic reticulum stress
In Vitro Cell Dev Biol Anim. 2020 Sep;56(8):622-634.;doi: 10.1007/s11626-020-00478-x.

252. Shuhua Cao,Xuezhao Li,Yong Gao,Fahui Li,Kaoxue Li,Xuanxuan Cao,Yiwen Dai,Lirong Mao,Shanshan Wang,Xishi Tai
A simultaneously GSH-depleted bimetallic Cu(ii) complex for enhanced chemodynamic cancer therapy
Dalton Trans. 2020 Sep 14;49(34):11851-11858.;doi: 10.1039/d0dt01742f.

253. Yunhua Yu,Fei Wang,Jianjun Wang,Dongmei Zhang,Xianxian Zhao
Ketogenic diet attenuates aging-associated myocardial remodeling and dysfunction in mice
Exp Gerontol. 2020 Oct 15;140:111058.;doi: 10.1016/j.exger.2020.111058.

254. Zhen-Dong Zhang,Ya-Jun Yang,Xi-Wang Liu,Zhe Qin,Shi-Hong Li,Jian-Yong Li
The Protective Effect of Aspirin Eugenol Ester on Paraquat-Induced Acute Liver Injury Rats
Front Med (Lausanne). 2020 Dec 17;7:589011.;doi: 10.3389/fmed.2020.589011.

255. Hongdong Ma,Xindong Wang,Weilin Zhang,Haitian Li,Wei Zhao,Jun Sun,Maowei Yang
Melatonin Suppresses Ferroptosis Induced by High Glucose via Activation of the Nrf2/HO-1 Signaling Pathway in Type 2 Diabetic Osteoporosis
Oxid Med Cell Longev. 2020 Dec 4;2020:9067610.;doi: 10.1155/2020/9067610.

256. Jian-Shu Lou,Li-Ping Zhao,Zhi-Hui Huang,Xia-Yin Chen,Jing-Ting Xu,William Chi-Shing Tai,Karl W K Tsim,Yi-Tao Chen,Tian Xie
Ginkgetin derived from Ginkgo biloba leaves enhances the therapeutic effect of cisplatin via ferroptosis-mediated disruption of the Nrf2/HO-1 axis in EGFR wild-type non-small-cell lung cancer
Phytomedicine. 2021 Jan;80:153370.;doi: 10.1016/j.phymed.2020.153370.

过氧化氢检测试剂盒(S0038)

过氧化氢检测试剂盒

产品编号: S0038

产品包装:150次
选择包装
150次

说明书下载

985.00 10
价格: ¥ 985.00

产品简介
使用说明
产品图片
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
S0038 过氧化氢检测试剂盒 150次 985.00元

过氧化氢检测试剂盒(Hydrogen Peroxide Assay Kit)可以用于培养细胞或组织内过氧化氢水平的测定,也可以用于培养细胞的上清或血清、尿液、血浆或其它生物体液中的过氧化氢浓度的测定。
过氧化氢是一种活性氧代谢的副产物,在许多氧化应急反应中过氧化氢都是一种关键的调节因子。过氧化氢可以激活NF-κB等因子,这些过氧化氢相关的信号途径和哮喘、炎症性关节炎、动脉硬化以及神经退行性疾病等许多疾病相关。过氧化氢也和细胞凋亡、细胞增殖等密切相关。
本试剂盒通过过氧化氢氧化二价铁离子产生三价铁离子,然后和xylenol orange在特定的溶液中形成紫色的产物,从而实现对过氧化氢浓度的测定。本试剂盒经过改良配方,可以检测低达1微摩尔/升的过氧化氢。
本试剂盒方便快捷,通常10-20个样品可以在40-60分钟内测定完毕。
本试剂盒可以检测150个样品。
包装清单:

产品编号 产品名称 包装
S0038-1 过氧化氢检测试剂 15ml
S0038-2 过氧化氢标准溶液(1M) 1ml
S0038-3 过氧化氢检测裂解液 50ml
说明书 1份

保存条件:
-20℃保存,一年有效。其中过氧化氢标准溶液需避光保存。
注意事项:
一些干扰氧化还原的试剂或在酸性条件下呈紫色或接近的试剂会对过氧化氢的检测产生干扰,需尽量避免。
如果样品中含有外加的较高浓度的铁盐,会干扰测定。但普通培养基、血清等样品中含有的微量的铁盐不会干扰测定。
所测得的标准曲线尽管在一定的浓度范围内接近直线,但整个标准曲线不是直线。
测定时需可以测定A560的酶标仪一台(测540-570nm也可以)或可以测定微量样品的分光光度计一台。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1.样品测定的准备:
a.细胞或组织样品的制备
对于培养的细胞,先收集细胞到离心管内,弃上清,按照每100万细胞加入100-200微升过氧化氢检测裂解液的比例加入裂解液,随后充分匀浆以破碎并裂解细胞。4℃约12000g离心3-5分钟,取上清,用于后续测定。组织样品按照每5-10mg组织加入100-200微升裂解液的比例进行匀浆。4℃约12000g离心3-5分钟,取上清用于后续测定。以上所有操作均需在4℃或冰上操作。制备好的细胞或组织样品如果不立即测定,可以-20℃冻存。
b.培养细胞上清液样品的制备
培养细胞的上清液可以直接用于后续的测定。
c.血清、血浆或尿液样品的准备:
配制50mM磷酸缓冲液,pH为6.0。用pH为6.0的50mM磷酸缓冲液把样品稀释50倍。例如4微升样品稀释到196微升pH为6.0的50mM磷酸缓冲液中。稀释后即可用于后续的测定。
2.标准曲线测定的准备:
a.过氧化氢标准品的校准:
由于过氧化氢不是非常稳定,使用前需自行测定过氧化氢的实际浓度以进行校准。把浓度约为1M的过氧化氢用水稀释100倍,使过氧化氢的浓度约为10mM,测定A240。A240的测定可采用如下的任一方法:
(a)普通紫外分光光度计法:使用含比色皿架的紫外分光光度计、NanoDrop 2000C、NanoDrop Oneᶜ、QuickDrop等仪器,配套石英比色皿。确定比色皿光程(path length),一般为1cm。用比色皿检测的过氧化氢浓度最接近实际浓度。
(b)微量紫外分光光度计法:如NanoDrop 2000、NanoDrop One、QuickDrop、含超微量检测板µDrop Plate的Varioskan等仪器。确定光程:对于NanoDrop 2000、NanoDrop One等,需要取消“自动化光程”,此时光程一般为0.1cm;Varioskan的超微量检测板µDrop Plate的光程一般为0.05cm。具体的微量紫外分光光度计的光程请参考仪器参数。
(c)96孔紫外酶标仪法(须能检测240nm波长):根据96孔板的参数确定光程,一般200微升样品的光程为0.552cm (样品体积除以96孔单孔孔内横截面面积)。一般建议使用专用的96孔紫外检测板(如96孔UV板),如果没有紫外检测板,也可使用一般的96孔板,但由于为非紫外检测专用板,会有非常高的紫外吸收信号,所以需要设置含等量双蒸水的孔作为空白对照(一般200μl水在该类96孔板的A240在3.8左右),计算时须减去该空白对照。在使用非紫外检测专用板的情况下,由于96孔酶标仪在240nm的检测上限有限,建议将过氧化氢稀释至约10mM左右后再进行浓度测定。
注意:以上所有方法都需要设置等量双蒸水作为空白对照,并在计算时减去该空白对照。
浓度计算公式:c=A/(ε×b)。其中:c为样品浓度(单位为mol/L或M);A为吸光值;ε为波长依赖的摩尔消光系数(单位为L×mol-1×cm-1或M-1×cm-1),过氧化氢的摩尔消光系数为43.6M-1cm-1;b=光程(单位为cm)。
因此:过氧化氢浓度(M)=A240/(43.6×b);即:过氧化氢浓度(mM)=22.94 X A240/b
从而计算出本试剂盒提供的过氧化氢的实际浓度,并根据实际测定出来的浓度进行后续的标准曲线的设置。
示例:将本试剂盒提供的约为1M的过氧化氢用双蒸水稀释100倍后,用96孔酶标仪及一般的96孔板进行检测,每孔200微升,每组3个平行。双蒸水对照组的平均A240为3.750,过氧化氢样品组的平均A240为3.974,差值为0.224,200微升样品的光程为0.552cm。代入公式,过氧化氢浓度(mM)=22.94×0.224/0.552=9.31,则实际本试剂盒提供的过氧化氢浓度为0.931M。
b.标准曲线的设置:
样品在什么溶液中标准品也需用什么溶液稀释,这样可以减小误差。例如对于细胞样品,标准品宜用过氧化氢检测裂解液稀释,对于培养细胞的上清液样品,标准品宜用相应的细胞培养液稀释。标准溶液可以稀释成1、3、10、30、100微摩尔/升,或1、2、5、10、20、50、100微摩尔/升,初次测定后知道样品的浓度范围后可以对标准品在样品浓度范围附近密集测定。
3. 过氧化氢浓度的测定:
a.把过氧化氢检测试剂在冰上或冰水浴上融解。
b.在检测孔或检测管内加入50微升样品或标准品。
c.在每个孔内加入100微升过氧化氢检测试剂。
d.轻轻振荡或敲打混匀,室温(15-30℃)放置30分钟。然后立即测定A560。如测A560有困难,波长可以选择540-570nm。
e.根据标准曲线计算出样品中过氧化氢的浓度。
注意:如果样品中过氧化氢的浓度过高,可以适当稀释后再测定。如果样品中过氧化氢的浓度过低,可以把样品的体积改为使用100微升,同时标准品也使用100微升,而检测试剂仍然 使用100微升。这样可以提高检测的灵敏度,但缺点是样品需要消耗100微升。

过氧化氢检测试剂盒(S0038)

相关产品请点击如下按钮:
活性氧相关

使用本产品的文献:
1. Dai X, Sun Y, Jiang Z.
Protective Effects of Vitamin Eagainst Oxidative Damage Induced by Abeta(1-40)Cu(II) Complexes.
ACTA BIOCH BIOPH SIN . 2007 Feb;39(2):123-30. (IF 2.836)

2. Gao S, Liu C, Qu S, Song J, Li J, Zhang P, Wang Q, Guo C, Gao F, Zhang L.
Non-cell Corynebacterium parvum generated by nanotechnology: a promising immunomodulator with less side effects.
Int Immunopharmacol . 2007 Oct;7(10):1334-42. (IF 3.943)

3. Qian H, Chen W, Li J, Wang J, Zhou Z, Liu W, Fu Z.
The effect of exogenous nitric oxide on alleviating herbicide damage in Chlorella vulgaris.
Aquat Toxicol . 2009 May 17;92(4):250-7. (IF 4.344)

4. Sheng R, Gu ZL, Xie ML, Zhou WX, Guo CY.
Epigallocatechin gallate protects H9c2 cardiomyoblasts against hydrogen dioxides-induced apoptosis and telomere attrition.
Eur J Pharmacol . 2010;641(2-3):199-206. (IF 3.263)

5. Dai X, Sun Y, Gao Z, Jiang Z.
Copper Enhances Amyloid-beta Peptide Neurotoxicity and non beta-Aggregation: A Series of Experiments Conducted upon Copper-Bound and Copper-Free Amyloid-beta Peptide.
J Mol Neurosci . 2010 May;41(1):66-73. (IF 2.678)

6. Yang Q, Gong ZJ, Zhou Y, Yuan JQ, Cheng J, Tian L, Li S, Lin XD, Xu R, Zhu ZR, Mao C.
Role of Drosophila alkaline ceramidase (Dacer) in Drosophila development and longevity.
Cell Mol Life Sci . 2010 May;67(9):1477-90. (IF 6.496)

7. Dai X, Sun Y, Gao Z, Jiang Z.
Copper enhances amyloid-beta peptide neurotoxicity and non beta-aggregation: a series ofexperiments conducted upon copper-bound and copper-free amyloid-beta peptide.
J Mol Neurosci . 2010 May;41(1):66-73. (IF 2.678)

8. Huang HC, Lin CJ, Liu WJ, Jiang RR, Jiang ZF.
Dual effects of curcumin on neuronal oxidative stress in the presence of Cu(II).
Food Chem Toxicol . 2011 Jul;49(7):1578-83. (IF 4.679)

9. Jiang S, Zu Y, Wang Z, Zhang Y, Fu Y.
Involvement of mitochondrial permeability transition pore opening in 7-xylosyl-10-deacetylpaclitaxel-induced apoptosis.
Planta Med . 2011 Jul;77(10):1005-12. (IF 2.687)

10. Shi R, Hu C, Yuan Q, Yang T, Peng J, Li Y, Bai Y, Cao Z, Cheng G, Zhang G.
Involvement of vascular peroxidase 1 in angiotensin II-induced vascular smooth muscle cellproliferation.
Cardiovasc Res . 2011 Jul 1;91(1):27-36. (IF 8.168)

11. Dai X, Chang P, Liu W, Xu K, Sun Y, Zhu S, Jiang Z.
Aβ-40 Y10F Increases βfibrils Formation but Attenuates the Neurotoxicity of Amyloid-β Peptide.
Int J Mol Sci . 2012;13(5):5324-37. (IF 4.556)

12. Gong C, Tao G, Yang L, Liu J, He H, Zhuang Z.
The role of reactive oxygen species in silicon dioxide nanoparticle-induced cytotoxicity and DNAdamage in HaCaT cells.
Mol Biol Rep . 2012 Apr;39(4):4915-25. (IF 1.402)

13. Wang J, Wang Q, Li J, Shen Q, Wang F, Wang L.
Cadmium induces hydrogen peroxide production and initiates hydrogen peroxide-dependentapoptosis in the gill of freshwater crab, Sinopotamon henanense.
COMP BIOCHEM PHYS C . 2012 Nov;156(3-4):195-201. (IF 2.892)

14. Li R, Li H, Luo H, Lin Z, Jiang Z, Luo W.
SSAO inhibitors suppress hepatocellular tumor growth in mice.
Cellular Immunology . 2013 May–June;283(1-2):61–9. (IF 3.291)

15. Wang J, Zhang P, Shen Q, Wang Q, Liu D, Li J, Wang L.
The Effects of Cadmium Exposure on the Oxidative State and Cell Death in the Gill of Freshwater Crab Sinopotamon henanense.
PLoS One . 2013 May 30;8(5):e64020. (IF 2.74)

16. Dong J, Zhang M, Liao Z, Wu W, Wang T, Chen L, Yang T, Guo L, Xu D, Wen F.
Resolvin-D1 inhibits interleukin-8 and hydrogen peroxide production induced by cigarette smoke extract in 16HBE cells via attenuating NF-κB activation.
CHINESE MED J-PEKING . 2014;127(3):511-7. (IF 1.585)

17. Zhang L, Dong L, Liu X, Jiang Y, Zhang L, Zhang X, Li X, Zhang Y.
α-Melanocyte-stimulating hormone protects retinal vascular endothelial cells from oxidative stress and apoptosis in a rat model of diabetes.
PLoS One . 2014 Apr 2;9(4):e93433. (IF 2.74)

18. Meng H, Guo J, Wang H, Yan P, Niu X, Zhang J.
Erythropoietin activates Keap1-Nrf2/ARE pathway in rat brain after ischemia.
Int J Neurosci . 2014 May;124(5):362-8. (IF 2.107)

19. Ren M, He L, Huang Y, Mao Q, Li S, Qu H, Bian M, Liang P, Chen X, Ling J, Chen T, Liang C, Wang X, Li X, Yu X.
Molecular characterization of Clonorchis sinensis secretory myoglobin: delineating its role in anti-oxidative survival.
PARASITE VECTOR . 2014 May 29;7(1):250. (IF 2.824)

20. Liu J, Yin Y, Song Z, Li Y, Jiang S, Shao C, Wang Z.
NADH: flavin oxidoreductase/NADH oxidase and ROS regulate microsclerotium development in Nomuraea rileyi.
WORLD J MICROB BIOT . 2014 Jul;30(7):1927-35. (IF 2.477)

21. Chen H, Gong Y, Han R.
Cadmium Telluride Quantum Dots (CdTe-QDs) and Enhanced Ultraviolet-B (UV-B) Radiation Trigger Antioxidant Enzyme Metabolism and Programmed Cell Death in Wheat Seedlings.
PLoS One . 2014 Oct 20;9(10):e110400. (IF 2.74)

22. Zhang H, Liu Y, Wen F, Yao D, Wang L, Guo J, Ni L, Zhang A, Tan M, Jiang M.
A novel rice C2H2-type zinc finger protein, ZFP36, is a key player involved in abscisic acid-induced antioxidant defence and oxidative stress tolerance in rice.
J Exp Bot . 2014 Nov;65(20):5795-809. (IF 5.908)

23. Zhong S, Zhao L, Li Q, Yang P, Varghese Z, Moorhead JF, Chen Y, Ruan XZ.
Inflammatory Stress Exacerbated Mesangial Foam Cell Formation and Renal Injury via Disrupting Cellular Cholesterol Homeostasis.
Inflammation . 2015;38(3):959-71. (IF 3.212)

24. Li W, Cao L, Han L, Xu Q, Ma Q.
Superoxide dismutase promotes the epithelial-mesenchymal transition of pancreatic cancer cells via activation of the H2O2/ERK/NF-κB axis.
Int J Oncol . 2015;46(6):2613-20. (IF 3.899)

25. Wu F, Sheng P, Tan J, Chen X, Lu G, Ma W, Heng Y, Lin Q, Zhu S, Wang J, Wang J, Guo X, Zhang X, Lei C, Wan J.
Plasma membrane receptor-like kinase leaf panicle 2 acts downstream of the DROUGHT AND SALT TOLERANCE transcription factor to regulate drought sensitivity in rice.
J Exp Bot . 2015 Jan;66(1):271-81. (IF 5.908)

26. Wang W, Ding XQ, Gu TT, Song L, Li JM, Xue QC, Kong LD.
Pterostilbene and allopurinol reduce fructose-induced podocyte oxidative stress and inflammation via microRNA-377.
FREE RADICAL BIO MED . 2015 Jun;83:214-26. (IF 6.17)

27. Yang HT, Yang MC, Sun JJ, Guo F, Lan JF, Wang XW, Zhao XF, Wang JX.
Catalase eliminates reactive oxygen species and influences the intestinal microbiota of shrimp.
FISH SHELLFISH IMMUN . 2015 Nov;47(1):63-73. (IF 3.298)

28. Wang D, Ma Y, Yang X, Xu X, Zhao Y, Zhu Z, Wang X, Deng H, Li C, Gao F, Tong J, Yamanaka K, An Y.
Hypermethylation of the Keap1 gene inactivates its function, promotes Nrf2 nuclear accumulation, and is involved in arsenite-induced human keratinocyte transformation.
FREE RADICAL BIO MED . 2015 Dec;89:209-19. (IF 6.17)

29. Tang Y, Xu Q, Peng H, Liu Z, Yang T, Yu Z, Cheng G, Li X, Zhang G, Shi R.
The role of vascular peroxidase 1 in ox-LDL-induced vascular smooth muscle cell calcification.
Atherosclerosis . 2015 Dec;243(2):357-63. (IF 3.919)

30. Zhang Z, Huang T, Yu F, Liu X, Zhao C, Chen X, Kelvin DJ, Gu J.
Infectious Progeny of 2009 A (H1N1) Influenza Virus Replicated in and Released from Human Neutrophils.
SCI REP-UK . 2015 Dec 7;5:17809. (IF 3.998)

31. Zhang Y, Zhang Y.
Toll-like receptor-6 (TLR6) deficient mice are protected from myocardial fibrosis induced by high fructose feeding through anti-oxidant and inflammatory signaling pathway.
BIOCHEM BIOPH RES CO . 2016 Apr 29;473(2):388-95. (IF 2.985)

32. Shen Y, Miao NJ, Xu JL, Gan XX, Xu D, Zhou L, Xue H, Zhang W, Lu LM.
N-acetylcysteine alleviates angiotensin II-mediated renal fibrosis in mouse obstructed kidneys.
Acta Pharmacol Sin . 2016 May;37(5):637-44. (IF 5.064)

33. Ren H, Yang Z, Luo C, Zeng H, Li P, Kang JX, Wan JB, He C, Su H.
Enriched Endogenous Omega-3 Fatty Acids in Mice Ameliorate Parenchymal Cell Death After TraumaticBrain Injury.
Mol Neurobiol . 2016 May 11.[Epub ahead of print] (IF 4.5)

34. Shen J, Tian Y, Li Y, Ma R, Zhang Q, Zhang J, Fang J.
Bactericidal Effects against S. aureus and Physicochemical Properties of Plasma Activated Water stored atdifferent temperatures.
SCI REP-UK . 2016 Jun 27;6:28505. (IF 3.998)

35. Yang HT, Yang MC, Sun JJ, Shi XZ, Zhao XF, Wang JX.
Dual oxidases participate in the regulation of intestinal microbiotic homeostasis in the
Dev Comp Immunol . 2016 Jun;59:153-63. (IF 3.192)

36. Yang L, Yuan Y, Fu C, Xu X, Zhou J, Wang S, Kong L, Li Z, Guo Q, Wei L.
LZ-10, a novel analog of enoxacin, inducing apoptosis via activation of
FREE RADICAL BIO MED . 2016 Jun;95:155-68. (IF 6.17)

37. Wu B, Yu H, Wang Y, Pan Z, Zhang Y, Li T, Li L, Zhang W, Ge L, Chen Y, Ho CK, Zhu D, Huang X, Lou Y.
Peroxiredoxin-2 nitrosylation facilitates cardiomyogenesis of mouse embryonic stem cells via XBP-1s/PI3Kpathway.
FREE RADICAL BIO MED . 2016 Aug;97:179-91. (IF 6.17)

38. Zhu Z, Sun B, Xu X, Chen H, Zou L, Chen G, Cao B, Chen C, Lei J.
Overexpression of AtEDT1/HDG11 in Chinese Kale (Brassica oleracea var. alboglabra) Enhances Droughtand Osmotic Stress Tolerance.
Front Plant Sci . 2016 Aug 30;7:1285. (IF 4.402)

39. Wang Q, Zhou Y, Chen K, Ju X.
Identification and characterization of an atypical 2-cys peroxiredoxin from the silkworm, Bombyx mori.
Insect Mol Biol . 2016 Aug;25(4):347-54. (IF 2.533)

40. Li H, Sun JJ, Chen GY, Wang WW, Xie ZT, Tang GF, Wei SD.
Carnosic acid nanoparticles suppress liver ischemia/reperfusion injury by inhibition of ROS, Caspases and NF-κB signaling pathway in mice.
Biomed Pharmacother . 2016 Aug;82:237-46. (IF 4.545)

41. Huang Q, Wang Q, Zhang S, Jiang S, Zhao L, Yu L, Hultström M, Patzak A, Li L, Wilcox CS, Lai EY.
Increased hydrogen peroxide impairs angiotensin II contractions of afferent arterioles in mice after renalischaemia-reperfusion injury.
ACTA PHYSIOL . 2016 Oct;218(2):136-45. (IF 5.542)

42. Li K, Xie Y, You M, Huang L, Zheng X.
Cerium Oxide-Incorporated Calcium Silicate Coating Protects MC3T3-E1 Osteoblastic Cells from H2O2-Induced Oxidative Stress.
Biol Trace Elem Res . 2016 Nov;174(1):198-207. (IF 2.639)

43. Wang SQ, Wang C, Chang LM, Zhou KR, Wang JW, Ke Y, Yang DX, Shi HG, Wang R, Shi XL, Ma LY, Liu HM.
Geridonin and paclitaxel act synergistically to inhibit the proliferation of gastric cancer cells through ROS-mediated regulation of the PTEN/PI3K/Akt pathway.
ONCOTARGET . 2016 Nov 8;7(45):72990-73002. (IF 5.168)

44. Liu Q, Yang J, Yan S, Zhang S, Zhao J, Wang W, Yang T, Wang X, Mao X, Dong J, Zhu X, Liu B.
The germin-like protein OsGLP2-1 enhances resistance to fungal blast and bacterial blight in
Plant Mol Biol . 2016 Nov;92(4-5):411-423. (IF 3.302)

45. Xu ZQ, Shao BZ, Ke P, Liu JG, Liu GK, Chen XW, Su DF, Liu C.
Combined administration of anisodamine and neostigmine rescued acute lethal crush syndrome through α7nAChR-dependent JAK2-STAT3 signaling.
SCI REP-UK . 2016 Nov 22;6:37709. (IF 3.998)

46. Zhou B, Mu J, Gong Y, Lu C, Zhao Y, He T, Qin Z.
Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-β-mediated Nox4expression.
Redox Biol . 2016 Dec 30;11:390-402. (IF 9.986)

47. Zhang Y, Huang X, Zhao XY, Hu YJ, Sun HY, Kong WJ.
Role ofthe Ubiquitin C-Terminal Hydrolase L1-Modulated Ubiquitin Proteasome System in Auditory Cortex Senescence.
ORL J OTO-RHINO-LARY . 2017;79(3):153-163. (IF 1.08)

48. Liu X, Zhang X, Ding Y, Zhou W, Tao L, Lu P, Wang Y, Hu R.
Nuclear Factor E2-Related Factor-2 Negatively Regulates NLRP3 Inflammasome Activity by InhibitingReactive Oxygen Species-Induced NLRP3 Priming.
ANTIOXID REDOX SIGN . 2017 Jan 1;26(1):28-43. (IF 6.323)

49. Zhong S, Zhao L, Wang Y, Zhang C, Liu J, Wang P, Zhou W, Yang P, Varghese Z, Moorhead JF, Chen Y, Ruan XZ.
Cluster of Differentiation 36 Deficiency Aggravates Macrophage Infiltration and Hepatic Inflammation byUpregulating Monocyte Chemotactic Protein-1 Expression of Hepatocytes Through Histone Deacetylase 2-Dependent Pathway.
ANTIOXID REDOX SIGN . 2017 Jan 10.doi: 10.1089/ars.2016.6808. [Epub ahead of print] (IF 6.323)

50. Chen Z, Xie X, Huang J, Gong W, Zhu X, Chen Q, Huang J, Huang H.
Connexin43 regulates high glucose-induced expression of fibronectin, ICAM-1 and TGF-β1 via Nrf2/AREpathway in glomerular mesangial cells.
FREE RADICAL BIO MED . 2017 Jan;102:77-86. (IF 6.17)

51. Dong W, Liu Z, Qiu L, Wang W, Song X, Wang X, Li Y, Xin L, Wang L, Song L.
The modulation role of serotonin in Pacific oyster Crassostrea gigas in response to air exposure.
FISH SHELLFISH IMMUN . 2017 Jan 31. pii: S1050-4648(17)30056-6. (IF 3.298)

52. Zhang H, Chen MK, Li K, Hu C, Lu MH, Situ J.
Eupafolin nanoparticle improves acute renal injury induced by LPS through inhibiting ROS andinflammation.
Biomed Pharmacother . 2017 Jan;85:704-711. (IF 4.545)

53. Lu XL, Zhao CH, Yao XL, Zhang H.
Quercetin attenuates high fructose feeding-induced atherosclerosis by suppressing inflammation andapoptosis via ROS-regulated PI3K/AKT signaling pathway.
Biomed Pharmacother . 2017 Jan;85:658-671. (IF 4.545)

54. Jiang DQ, Wang Y, Li MX, Ma YJ, Wang Y.
SIRT3 in Neural Stem Cells Attenuates Microglia Activation-Induced Oxidative Stress Injury Through Mitochondrial Pathway.
Front Cell Neurosci . 2017 Jan 31;11:7. (IF 3.921)

55. Chen Z, Xie X, Huang J, Gong W, Zhu X, Chen Q, Huang J, Huang H.
Connexin43 regulates high glucose-induced expression of fibronectin, ICAM-1 andTGF-β1 viaNrf2/ARE pathway in glomerular mesangial cells.
FREE RADICAL BIO MED 2017 Jan;102:77-86. (IF 6.17)

56. Ma Y, Jiao J, Fan X, Sun H, Zhang Y, Jiang J, Liu C.
Endophytic Bacterium Pseudomonas fluorescens RG11 May Transform Tryptophan to Melatonin and Promote Endogenous Melatonin Levels in the Roots of Four Grape Cultivars.
Front Plant Sci . 2017 Jan 10;7:2068. (IF 4.402)

57. Zhang H, Chen MK, Li K, Hu C, Lu MH, Situ J.
Eupafolin nanoparticle improves acute renal injury induced by LPS through inhibiting ROS and inflammation.
Biomed Pharmacother . 2017 Jan;85:704-711. (IF 4.545)

58. Lu XL, Zhao CH, Yao XL, Zhang H.
Quercetin attenuates high fructose feeding-induced atherosclerosis by suppressing inflammation and apoptosis via ROS-regulated PI3K/AKT signaling pathway.
Biomed Pharmacother . 2017 Jan;85:658-671. (IF 4.545)

59. Zhu Z, Chen Y, Shi G, Zhang X.
Selenium delays tomato fruit ripening by inhibiting ethylene biosynthesis and enhancing the antioxidantdefense system.
Food Chem . 2017 Mar 15;219:179-184. (IF 6.306)

60. Zhu Z, Chen Y, Shi G, Zhang X.
Selenium delays tomato fruit ripening by inhibiting ethylene biosynthesis and enhancing the antioxidant defense system.
Food Chem . 2017 Mar 15;219:179-184. (IF 6.306)

61. Dai F, Li Q, Wang Y, Ge C, Feng C, Xie S, He H, Xu X, Wang C.
Design, Synthesis,and Biological Evaluation of Mitochondria-Targeted Flavone-Naphthalimide-Polyamine Conjugates with Antimetastatic Activity.
J Med Chem . 2017 Mar 9;60(5):2071-2083. (IF 6.205)

62. Xie Y, Han S, Li X, Amombo E, Fu J.
Amelioration of Salt Stress on Bermudagrass by the Fungus Aspergillus aculeatus.
MOL PLANT MICROBE IN . 2017 Mar;30(3):245-254. (IF 3.696)

63. Gong W, Li J, Chen Z, Huang J, Chen Q, Cai W, Liu P, Huang H.
Polydatin promotes Nrf2-ARE anti-oxidative pathway through activating CKIP-1 to resist HG-induced up-regulation of FN and ICAM-1 in GMCs and diabetic mice kidneys.
FREE RADICAL BIO MED . 2017 May;106:393-405. (IF 6.17)

64. Yao W, Zhao H, Shi R, Li X, Li Y, Ke C, Liu J.
Recombinant protein transduction domain-Cu/Zn superoxide dismutase alleviates bone cancerpain via peroxiredoxin 4 modulation and antioxidation.
BIOCHEM BIOPH RES CO . 2017 May 13;486(4):1143-1148. (IF 2.985)

65. Chen J, Wang YX, Dong MQ, Zhang B, Luo Y, Niu W, Li ZC.
Reoxygenation Reverses Hypoxic Pulmonary Arterial Remodeling by Inducing Smooth MuscleCell Apoptosis via Reactive Oxygen Species-Mediated Mitochondrial Dysfunction.
J Am Heart Assoc . 2017 Jun 23;6(6). (IF 4.605)

66. Wei G, Lai Y, Wang G, Chen H, Li F, Wang S.
Insect pathogenic fungus interacts with the gut microbiota to accelerate mosquito mortality.
P NATL ACAD SCI USA . 2017 Jun 6;114(23):5994-5999. (IF 9.412)

67. Chen YH, Liu SJ, Gao MM, Zeng T, Lin GW, Tan NN, Tang HL, Lu P, Su T, Sun WW, Xie LC, Yi YH, Long YS.
MDH2 isan RNA binding protein involved in downregulation of sodium channel Scn1aexpression under seizure condition.
BBA-BIOMEMBRANES . 2017 Jun;1863(6):1492-1499. (IF 3.411)

68. Zhu Z, Ren Z, Fan X, Pan Y, Lv S, Pan C, Lei A, Zeng W.
Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages.
PLoS One . 2017 Jul 10;12(7):e0181110. (IF 2.74)

69. Wang B, Xiong S, Lin S, Xia W, Li Q, Zhao Z, Wei X, Lu Z, Wei X, Gao P, Liu D, Zhu Z.
Enhanced Mitochondrial Transient Receptor Potential Channel, Canonical Type 3-MediatedCalcium Handling in the Vasculature From Hypertensive Rats.
J Am Heart Assoc . 2017 Jul 15;6(7). pii: e005812. (IF 4.605)

70. Liu Z, Liu Y, Xu Q, Peng H, Tang Y, Yang T, Yu Z, Cheng G, Zhang G, Shi R.
Critical role of vascular peroxidase 1 in regulating endothelial nitric oxide synthase.
Redox Biol . 2017 Aug;12:226-232. (IF 9.986)

71. Meng W, Pei Z, Feng Y, Zhao J, Chen Y, Shi W, Xu Q, Lin F, Sun M, Xiao K.
Neglected role of hydrogen sulfide in sulfur mustard poisoning: Keap1 S-sulfhydration and subsequent Nrf2 pathway activation.
SCI REP-UK . 2017 Aug 25;7(1):9433. (IF 3.998)

72. Zhong S, Zhao L, Wang Y, Zhang C, Liu J, Wang P, Zhou W, Yang P, Varghese Z, Moorhead JF, Chen Y, Ruan XZ.
Cluster of Differentiation 36 Deficiency Aggravates Macrophage Infiltration and HepaticInflammation by Upregulating Monocyte Chemotactic Protein-1 Expression of Hepatocytes Through Histone Deacetylase 2-Dependent Pathway.
ANTIOXID REDOX SIGN . 2017 Aug 1;27(4):201-214. (IF 6.323)

73. Tang S, Li L, Wang Y, Chen Q, Zhang W, Jia G, Zhi H, Zhao B, Diao X.
Genotype-specific physiological and transcriptomic responses to drought stress in Setaria italica (an emerging model for Panicoideae grasses).
SCI REP-UK . 2017 Aug 30;7(1):10009. (IF 3.998)

74. Lv Y, Cheng X, Du G, Zhou J, Chen J.
Engineering of an H2O2 auto-scavenging in vivo cascade for pinoresinol production.
Biotechnol Bioeng . 2017 Sep;114(9):2066-2074. (IF 4.002)

75. Li X, Han S, Wang G, Liu X, Amombo E, Xie Y, Fu J.
The Fungus Aspergillus aculeatus Enhances Salt-Stress Tolerance, Metabolite Accumulation, and Improves Forage Quality in Perennial Ryegrass.
Front Microbiol . 2017 Sep 4;8:1664. (IF 4.235)

76. Lv Y,Cheng X,Du G,Zhou J,Chen J
Engineering of an H2 O2 auto-scavenging in vivo cascade for pinoresinol production.
Biotechnol Bioeng . 2017 Sep;114(9):2066-2074. (IF 4.002)

77. Luo X, Dan Wang, Luo X, Zhu X, Wang G, Ning Z, Li Y, Ma X, Yang R, Jin S, Huang Y, Meng Y, Li X.
Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liversinusoidal endothelial cells defenestration.
Redox Biol . 2017 Oct;13:508-521. (IF 9.986)

78. Tang G, Zhang C, Ju Z, Zheng S, Wen Z, Xu S, Chen Y, Ma Z.
The mitochondrial membrane protein FgLetm1 regulates mitochondrial integrity, production of endogenous reactive oxygen species and mycotoxin biosynthesis in Fusarium graminearum.
Mol Plant Pathol . 2017 Oct 27. (IF 4.326)

79. Zou Y, Xiong JB, Ma K, Wang AZ, Qian KJ.
Rac2 deficiency attenuates CCl4-induced liver injury through suppressing inflammation and oxidative stress.
Biomed Pharmacother . 2017 Oct;94:140-149. (IF 4.545)

80. Meng M.
Digitoflavone (DG) attenuates LPS-induced acute lung injury through reducing oxidative stress and inflammatory response dependent on the suppression of TXNIP/NLRP3 and NF-κB.
Biomed Pharmacother . 2017 Oct;94:712-725. (IF 4.545)

81. Liu M, Du G, Liu B, Hu Y, Liu J, Jia Y, Minion FC, Shao G, Zhao R.
Cholesterol exacerbates Mycoplasma hyopneumoniae-induced apoptosis via stimulatingproliferation and adhesion to porcine alveolar macrophages.
Vet Microbiol . 2017 Nov;211:112-118. (IF 3.03)

82. Yang HT, Zou SS, Zhai LJ, Wang Y, Zhang FM, An LG, Yang GW.
Pathogen invasion changes the intestinal microbiota composition and induces innate immuneresponses in the zebrafish intestine.
FISH SHELLFISH IMMUN . 2017 Dec;71:35-42. (IF 3.298)

83. Ma J, Chen J, Wang M, Ren Y, Wang S, Lei C, Cheng Z, Sodmergen.
Disruption of OsSEC3A increases the content of salicylicacid and induces plant defenseresponses in rice.
J Exp Bot . 2017 Dec 30. (IF 5.908)

84. Li Y, Pan J, Ye G, Zhang Q, Wang J, Zhang J, Fang J.
In vitro studies ofthe antimicrobial effect of non-thermal plasma-activated water asa novelmouthwash.
Eur J Oral Sci . 2017 Dec;125(6):463-470. (IF 2.22)

85. Xu S, Yang X, Qian Y, Xiao Q.
Parkinson disease-related DJ-1 modulates the expression of uncoupling protein 4 againstoxidative stress.
J Neurochem . 2018 Jan 8. (IF 4.066)

86. Zhang L,Wang J,Chen Y,Zeng L,Li Q,Liu Y,Wang L
Thioredoxin-1 Protects Bone Marrow-Derived Mesenchymal Stromal Cells from Hyperoxia-Induced Injury In Vitro.
Oxid Med Cell Longev . 2018 Jan 21;2018:1023025. (IF 5.076)

87. Su X, Tian Y, Zhou H, Li Y, Zhang Z, Jiang B, Yang B, Zhang J, Fang J.
Inactivation efficacy of non-thermal plasma activated solutions against Newcastle disease virus.
APPL ENVIRON MICROB . 2018 Feb 23. (IF 4.016)

88. Qu C, Li J, Zhou Y, Yang S, Chen W, Li F, You B, Liu Y, Zhang X.
Targeted Delivery of Doxorubicin via CD147-Mediated ROS/pH Dual-Sensitive Nanomicelles for the Efficient Therapy of Hepatocellular Carcinoma.
AAPS J . 2018 Feb 23;20(2):34. (IF 3.737)

89. Gong W, Chen Z, Zou Y, Zhang L, Huang J, Liu P, Huang H.
CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.
FREE RADICAL BIO MED . 2018 Feb 1;115:338-350. (IF 6.17)

90. Liao X, Li J, Muhammad AI, Suo Y, Chen S, Ye X, Liu D, Ding T.
Application ofa Dielectric Barrier Discharge Atmospheric Cold Plasma (Dbd-Acp)for EshcerichiaColi Inactivation in Apple Juice.
J Food Sci . 2018 Feb;83(2):401-408. (IF 2.478)

91. Zhang XT,Sun XQ,Wu C,Chen JL,Yuan JJ,Pang QF,Wang ZP
Heme oxygnease-1 induction by methylene blue protects RAW264.7 cells from hydrogen peroxide-induced injury.
Biochem Pharmacol . 2018 Feb;148:265-277. (IF 4.96)

92. Liao X,Li J,Muhammad AI,Suo Y,Chen S,Ye X,Liu D,Ding T
Application of a Dielectric Barrier Discharge Atmospheric Cold Plasma (Dbd-Acp) for Eshcerichia Coli Inactivation in Apple Juice.
J Food Sci . 2018 Feb;83(2):401-408. (IF 2.478)

93. Li W, Jiang Z, Xiao X, Wang Z, Wu Z, Ma Q, Cao L.
Curcumin inhibits superoxide dismutase-induced epithelial-to-mesenchymal transition via the PI3K/Akt/NF-κB pathway in pancreatic cancer cells.
Int J Oncol . 2018 Mar 1. (IF 3.899)

94. Qiu M,Shi F,Dai F,Song R,Wang S,You Y,Zhao B
A reactive oxygen species activation mechanism contributes to Sophoridine-induced apoptosis in rat liver BRL-3A cells.
J Ethnopharmacol . 2018 Mar 1;213:376-383. (IF 3.69)

95. Zang Y,Liu J,Tang XX,Zhou B
Description of a Zostera marina catalase gene involved in responses to temperature stress.
PeerJ . 2018 Mar 26;6:e4532. (IF 2.379)

96. Zhou S,Hong Q,Li Y,Li Q,Wang M
Autophagy contributes to regulate the ROS levels and PCD progress in TMV-infected tomatoes.
Plant Sci . 2018 Apr;269:12-19. (IF 3.591)

97. Liu S,Zhang C,Chao N,Lu J,Zhang Y
Cloning, Characterization, and Functional Investigation of VaHAESA from Vitis amurensis Inoculated with Plasmopara viticola.
Int J Mol Sci . 2018 Apr 16;19(4). pii: E1204. (IF 4.556)

98. Su X,Tian Y,Zhou H,Li Y,Zhang Z,Jiang B,Yang B,Zhang J,Fang J
Inactivation Efficacy of Nonthermal Plasma-Activated Solutions against Newcastle Disease Virus.
APPL ENVIRON MICROB . 2018 Apr 16;84(9). pii: e02836-17. (IF 4.016)

99. Huang L, Zhang M, Jia J, Zhao X, Huang X, Ji E, Ni L, Jiang M.
An Atypical Late Embryogenesis Abundant Protein OsLEA5 Plays A Positive Role In ABA-Induced Antioxidant Defense In Oryza Sativa L.
Plant Cell Physiol. . 2018 May 1;59(5):916-929

100. Xu S,Yang X,Qian Y,Xiao Q
Parkinson’s disease-related DJ-1 modulates the expression of uncoupling protein 4 against oxidative stress.
J Neurochem . 2018 May;145(4):312-322. (IF 4.066)

101. Li Y,Li Q,Hong Q,Lin Y,Mao W,Zhou S
Reactive oxygen species triggering systemic programmed cell death process via elevation of nuclear calcium ion level in tomatoes resisting tobacco mosaic virus.
Plant Sci . 2018 May;270:166-175. (IF 3.591)

102. Zhu J,Sun Y,Lu Y,Jiang X,Ma B,Yu L,Zhang J,Dong X,Zhang Q
Glaucocalyxin A exerts anticancer effect on osteosarcoma by inhibiting GLI1 nuclear translocation via regulating PI3K/Akt pathway.
Cell Death Dis . 2018 Jun 13;9(6):708. (IF 6.304)

103. Leng Y,Wu Y,Lei S,Zhou B,Qiu Z,Wang K,Xia Z
Inhibition of HDAC6 Activity Alleviates Myocardial Ischemia/Reperfusion Injury in Diabetic Rats: Potential Role of Peroxiredoxin 1 Acetylation and Redox Regulation.
Oxid Med Cell Longev . 2018 Jun 25;2018:9494052. (IF 5.076)

104. Zhang Y,Li Q,Fang M,Ma Y,Liu N,Yan X,Zhou J,Li F
The Kidney Injury Induced by Short-Term PM2.5 Exposure and the Prophylactic Treatment of Essential Oils in BALB/c Mice.
Oxid Med Cell Longev . 2018 Jul 29;2018:9098627. (IF 5.076)

105. Citao Liu,Shujun Ou,Bigang Mao,Jiuyou Tang,Wei Wang,Hongru Wang,Shouyun Cao,Michael R Schläppi,Bingran Zhao,Guoying Xiao,Xiping Wang,Chengcai Chu
Early Selection of bZIP73 Facilitated Adaptation of Japonica Rice to Cold Climates
Nat Commun. 2018 Aug 17;9(1):3302.;doi: 10.1038/s41467-018-05753-w. (IF 12.121)

106. Yin Y,Wang Z,Cheng D,Chen X,Chen Y,Ma Z
The ATP-binding protein FgArb1 is essential for penetration, infectious and normal growth of Fusarium graminearum.
New Phytol . 2018 Sep;219(4):1447-1466. (IF 8.512)

107. Han X,Wu YC,Meng M,Sun QS,Gao SM,Sun H
Linarin prevents LPS‑induced acute lung injury by suppressing oxidative stress and inflammation via inhibition of TXNIP/NLRP3 and NF‑κB pathways.
Int J Mol Med . 2018 Sep;42(3):1460-1472. (IF 3.098)

108. Yin Y,Wu S,Chui C,Ma T,Jiang H,Hahn M,Ma Z
The MAPK kinase BcMkk1 suppresses oxalic acid biosynthesis via impeding phosphorylation of BcRim15 by BcSch9 in Botrytis cinerea.
PLoS Pathog . 2018 Sep 13;14(9):e1007285. (IF 6.218)

109. Wang L,Gao H,Yang X,Liang X,Tan Q,Chen Z,Zhao C,Gu Z,Yu M,Zheng Y,Huang Y,Zhu L,Jacob TJC,Wang L,Chen L
The apoptotic effect of Zoledronic acid on the nasopharyngeal carcinoma cells via ROS mediated chloride channel activation.
CLIN EXP PHARMACOL P  . 2018 Oct;45(10):1019-1027. (IF 2.092)

110. Li J,Ma L,Liao X,Liu D,Lu X,Chen S,Ye X,Ding T
Ultrasound-Induced Escherichia coli O157:H7 Cell Death Exhibits Physical Disruption and Biochemical Apoptosis.
Front Microbiol . 2018 Oct 16;9:2486. (IF 4.235)

111. Yu Z,Dong L,Jiang Z,Yi K,Zhang J,Zhang Z,Zhu Z,Wu Y,Xu M,Ni J
A semi-dominant mutation in a CC-NB-LRR-type protein leads to a short-root phenotype in rice.
Rice (N Y) . 2018 Oct 3;11(1):54.

112. Jin Y,Yang Q,Liang L,Ding L,Liang Y,Zhang D,Wu B,Yang T,Liu H,Huang T,Shen H,Tu H,Pan Y,Wei Y,Yang Y,Zhou F
Compound kushen injection suppresses human acute myeloid leukaemia by regulating the Prdxs/ROS/Trx1 signalling pathway.
J EXP CLIN CANC RES . 2018 Nov 19;37(1):277. (IF 7.068)

113. Deng W,Duan M,Qian B,Zhu Y,Lin J,Zheng L,Zhang C,Qi X,Luo M
NADPH oxidase 1/4 inhibition attenuates the portal hypertensive syndrome via modulation of mesenteric angiogenesis and arterial hyporeactivity in rats.
CLIN RES HEPATOL GAS . 2018 Nov 6. pii: S2210-7401(18)30220-1. (IF 2.718)

114. Meng Q,Xu J,Liang C,Liu J,Hua J,Zhang Y,Ni Q,Shi S,Yu X
GPx1 is involved in the induction of protective autophagy in pancreatic cancer cells in response to glucose deprivation.
Cell Death Dis . 2018 Dec 11;9(12):1187. (IF 6.304)

115. Liang F,Su F,Wang X,Long S,Zheng Y,He X,Pang J,Pei Z
Xyloketal derivative C53N protects against mild traumatic brain injury in mice.
DRUG DES DEV THER. 2018 Dec 27;13:173-182. (IF 3.216)

116. Zhou R,Wang H,Yang Y,Zhang C,Dong X,Du J,Yan L,Zhang G,Gu Z,Zhao Y
Tumor microenvironment-manipulated radiocatalytic sensitizer based on bismuth heteropolytungstate for radiotherapy enhancement.
Biomaterials. 2019 Jan;189:11-22. (IF 10.317)

117. Zeng SY,Yang L,Yan QJ,Gao L,Lu HQ,Yan PK
Nox1/4 dual inhibitor GKT137831 attenuates hypertensive cardiac remodelling associating with the inhibition of ADAM17-dependent proinflammatory cytokines-induced signalling pathways in the rats with a
Biomed Pharmacother. 2019 Jan;109:1907-1914. (IF 4.545)

118. Zhang L,Zhao P,Yue C,Jin Z,Liu Q,Du X,He Q
Sustained release of bioactive hydrogen by Pd hydride nanoparticles overcomes Alzheimer’s disease.
Biomaterials. 2019 Mar;197:393-404. (IF 10.317)

119. You Y,Huang Y,Wang D,Li Y,Wang G,Jin S,Zhu X,Wu B,Du X,Li X
Angiotensin (1-7) inhibits arecoline-induced migration and collagen synthesis in human oral myofibroblasts via inhibiting NLRP3 inflammasome activation.
J Cell Physiol. 2019 Apr;234(4):4668-4680. (IF 5.546)

120. Xin L,Huang B,Zhang H,Li C,Bai C,Wang C
OsHV-1 infection leads to mollusc tissue lesion and iron redistribution, revealing a strategy of iron limitation against pathogen.
Metallomics. 2019 Apr 17;11(4):822-832. (IF 3.796)

121. Du X,Zhang T,Ma G,Gu X,Wang G,Li J
Glucose-responsive mesoporous silica nanoparticles to generation of hydrogen peroxide for synergistic cancer starvation and chemistry therapy.
INT J NANOMED. 2019 Apr 2;14:2233-2251. (IF 5.115)

122. Chen C,Jiang X,Li Y,Yu H,Li S,Zhang Z,Xu H,Yang Y,Liu G,Zhu F,Ren X,Zou L,Xu B,Liu J,Spencer PS,Yang X
Low-dose oral copper treatment changes the hippocampal phosphoproteomic profile and perturbs mitochondrial function in a mouse model of Alzheimer’s disease.
FREE RADICAL BIO MED. 2019 May 1;135:144-156. (IF 6.17)

123. Zhong J,Xie J,Xiao J,Li D,Xu B,Wang X,Wen H,Zhou Z,Cheng Y,Xu J,Wang H
Inhibition of PDE4 by FCPR16 induces AMPK-dependent autophagy and confers neuroprotection in SH-SY5Y cells and neurons exposed to MPP+-induced oxidative insult.
FREE RADICAL BIO MED. 2019 May 1;135:87-101. (IF 6.17)

124. Liao X,Liu D,Chen S,Ye X,Ding T
Degradation of antibiotic resistance contaminants in wastewater by atmospheric cold plasma: kinetics and mechanisms.
Environ Technol. 2019 May 27:1-14; (IF 2.213)

125. Han D,Gu X,Gao J,Wang Z,Liu G,Barkema HW,Han B
Chlorogenic acid promotes the Nrf2/HO-1 anti-oxidative pathway by activating p21Waf1/Cip1 to resist dexamethasone-induced apoptosis in osteoblastic cells.
FREE RADICAL BIO MED. 2019 Jun;137:1-12. (IF 6.17)

126. Zeng SY,Yang L,Lu HQ,Yan QJ,Gao L,Qin XP
Rutaecarpine prevents hypertensive cardiac hypertrophy involving the inhibition of Nox4-ROS-ADAM17 pathway.
J Cell Mol Med. 2019 Jun;23(6):4196-4207. (IF 4.486)

127. Tang Y,Huang Q,Liu C,Ou H,Huang D,Peng F,Liu C,Mo Z
p22phox promotes Ang-II-induced vascular smooth muscle cell phenotypic switch by regulating KLF4 expression.
BIOCHEM BIOPH RES CO. 2019 Jun 18;514(1):280-286. (IF 2.985)

128. Gong F,Gao L,Ding T
IDH2 protects against nonalcoholic steatohepatitis by alleviating dyslipidemia regulated by oxidative stress.
BIOCHEM BIOPH RES CO. 2019 Jun 30;514(3):593-600. (IF 2.985)

129. Wu Y,Guo T,Qiu Y,Lin Y,Yao Y,Lian W,Lin L,Song J,Yang H
An inorganic prodrug, tellurium nanowires with enhanced ROS generation and GSH depletion for selective cancer therapy.
Chem Sci. 2019 Jun 6;10(29):7068-7075. (IF 9.346)

130. Zhang P,Wang XL,Yang ZH,Su XJ,Wang XH
A novel rat model of seminal vesiculitis.
Asian J Androl. 2019 Jul-Aug;21(4):360-364. (IF 2.448)

131. Yang N,Ma H,Jiang Z,Niu L,Zhang X,Liu Y,Wang Y,Cheng S,Deng Y,Qi H,Wang Z
Dosing depending on SIRT3 activity attenuates doxorubicin-induced cardiotoxicity via elevated tolerance against mitochondrial dysfunction and oxidative stress.
BIOCHEM BIOPH RES CO. 2019 Sep 10;517(1):111-117. (IF 2.985)

132. Zhou TJ,Xing L,Fan YT,Cui PF,Jiang HL
Inhibition of breast cancer proliferation and metastasis by strengthening host immunity with a prolonged oxygen-generating phototherapy hydrogel.
J Control Release. 2019 Sep 10;309:82-93. (IF 7.727)

133. Hu JJ,Liu MD,Gao F,Chen Y,Peng SY,Li ZH,Cheng H,Zhang XZ
Photo-controlled liquid metal nanoparticle-enzyme for starvation/photothermal therapy of tumor by win-win cooperation.
Biomaterials. 2019 Oct;217:119303. (IF 10.317)

134. Zhou G,Wang YS,Peng H,Shen PF,Xie XB,Shi QS
Functional roles of norCB in Pseudomonas aeruginosa ATCC 9027 under aerobic conditions.
J BASIC MICROB. 2019 Nov;59(11):1154-1162. (IF 1.909)

135. Tian Z,Yang K,Yao T,Li X,Ma Y,Qu C,Qu X,Xu Y,Guo Y,Qu Y
Catalytically Selective Chemotherapy from Tumor-Metabolic Generated Lactic Acid.
Small. 2019 Nov;15(46):e1903746. (IF 11.459)

136. Jun Tong,Yuanmeng Jin,Qinjie Weng,Shuwen Yu,Hafiz Muhammad Jafar Hussain,Hong Ren,Jing Xu,Wen Zhang,Xiao Li,Weiming Wang,Jingyuan Xie,Nan Chen
Glomerular Transcriptome Profiles in Focal Glomerulosclerosis: New Genes and Pathways for Steroid Resistance
Am J Nephrol. 2020;51(6):442-452.;doi: 10.1159/000505956.

137. Huiying Zhang,Xin Liu,Xiyong Zhang,Ningning Qin,Kaifang Xu,Weihua Yin,Yueqin Zheng,Yuanyuan Song,Rensen Zeng,Jian Liu
Phosphoinositide 3-Kinase Promotes Oxidative Burst, Stomatal Closure and Plant Immunity in Bacterial Invasion
Front Plant Sci. 2020 Jan 24;10:1740.;doi: 10.3389/fpls.2019.01740.

138. Yongjian Guo,Libin Wei,Yuxin Zhou,Na Lu,Xiaoqing Tang,Zhiyu Li,Xiaotang Wang
Flavonoid GL-V9 induces apoptosis and inhibits glycolysis of breast cancer via disrupting GSK-3β-modulated mitochondrial binding of HKII
Free Radic Biol Med. 2020 Jan;146:119-129.;doi: 10.1016/j.freeradbiomed.2019.10.413.

139. Yang X,Zhao H,Luo C,Du L,Cheng J,Xie J,Jiang D,Fu Y
CmAim24 Is Essential for Mitochondrial Morphology, Conidiogenesis, and Mycoparasitism in Coniothyrium minitans.
APPL ENVIRON MICROB. 2020 Feb 18;86(5). pii: e02291-19. (IF 4.016)

140. Xinyu Liao,Donghong Liu,Tian Ding
Nonthermal Plasma Induces the Viable-but-Nonculturable State in Staphylococcus aureus via Metabolic Suppression and the Oxidative Stress Response
Appl Environ Microbiol. 2020 Feb 18;86(5):e02216-19.;doi: 10.1128/AEM.02216-19.

141. Rongrong Ni,Guojing Song,Xiaohong Fu,Ruifeng Song,Lanlan Li,Wendan Pu,Jining Gao,Jun Hu,Qin Liu,Fengtian He,Dinglin Zhang,Gang Huang
Reactive oxygen species-responsive dexamethasone-loaded nanoparticles for targeted treatment of rheumatoid arthritis via suppressing the iRhom2/TNF-α/BAFF signaling pathway
Biomaterials. 2020 Feb;232:119730.;doi: 10.1016/j.biomaterials.2019.119730.

142. Yu Zhang,Liming Li,Jiafu Mu,Jiachen Chen,Shiqing Feng,Jianqing Gao
Implantation of a functional TEMPO-hydrogel induces recovery from rat spinal cord transection through promoting nerve regeneration and protecting bladder tissue
Biomater Sci. 2020 Mar 17;8(6):1695-1701.;doi: 10.1039/c9bm01530b.

143. Yuanyang Dong,Qihang Hou,Jiaqi Lei,Patricia G Wolf,Hammed Ayansola,Bingkun Zhang
Quercetin Alleviates Intestinal Oxidative Damage Induced by H 2 O 2 via Modulation of GSH: In Vitro Screening and In Vivo Evaluation in a Colitis Model of Mice
ACS Omega. 2020 Apr 2;5(14):8334-8346.;doi: 10.1021/acsomega.0c00804.

144. Syed Adeel Zafar,Amjad Hameed,Muhammad Ashraf,Abdus Salam Khan,Zia-Ul- Qamar,Xueyong Li,Kadambot H M Siddique
Agronomic, physiological and molecular characterisation of rice mutants revealed the key role of reactive oxygen species and catalase in high-temperature stress tolerance
Funct Plant Biol. 2020 Apr;47(5):440-453.;doi: 10.1071/FP19246.

145. Boya Zhang,Shiyu Wan,Xinyu Peng,Mingying Zhao,Sai Li,Yuji Pu,Bin He
Human serum albumin-based doxorubicin prodrug nanoparticles with tumor pH-responsive aggregation-enhanced retention and reduced cardiotoxicity
J Mater Chem B. 2020 May 6;8(17):3939-3948.;doi: 10.1039/d0tb00327a.

146. Xue-Jun Chen,Lei Wang,Xiao-Yang Song
Mitoquinone alleviates vincristine-induced neuropathic pain through inhibiting oxidative stress and apoptosis via the improvement of mitochondrial dysfunction
Biomed Pharmacother. 2020 May;125:110003.;doi: 10.1016/j.biopha.2020.110003.

147. Juan Wu,Nan Zhang,Ziguang Liu,Shengyi Liu,Chunxiao Liu,Jianhui Lin,He Yang,Shuang Li,Yasushi Yukawa
The AtGSTU7 gene influences glutathione-dependent seed germination under ABA and osmotic stress in Arabidopsis
Biochem Biophys Res Commun. 2020 Jul 30;528(3):538-544.;doi: 10.1016/j.bbrc.2020.05.153.

148. Ruilong Li,Weidong Yang
Gomisin J inhibits the glioma progression by inducing apoptosis and reducing HKII-regulated glycolysis
Biochem Biophys Res Commun. 2020 Aug 13;529(1):15-22.;doi: 10.1016/j.bbrc.2020.05.109.

149. Si-Yu Zeng,Qiu-Jiang Yan,Li Yang,Qing-Hua Mei,Hui-Qin Lu
Inhibition of the ROS-EGFR Pathway Mediates the Protective Action of Nox1/4 Inhibitor GKT137831 against Hypertensive Cardiac Hypertrophy via Suppressing Cardiac Inflammation and Activation of Akt and
Mediators Inflamm. 2020 Aug 4;2020:1078365.;doi: 10.1155/2020/1078365.

150. Tianlei Sun,Cheryl H T Kwong,Cheng Gao,Jianwen Wei,Ludan Yue,Jianxiang Zhang,Richard Dequan Ye,Ruibing Wang
Amelioration of ulcerative colitis via inflammatory regulation by macrophage-biomimetic nanomedicine
Theranostics. 2020 Aug 8;10(22):10106-10119.;doi: 10.7150/thno.48448.

151. Zhicheng Zhang,Li Wang,Yawen Ding,Jinhui Wu,Yiqiao Hu,Ahu Yuan
Synergy of hypoxia relief and chromatin remodeling to overcome tumor radiation resistance
Biomater Sci. 2020 Sep 7;8(17):4739-4749.;doi: 10.1039/d0bm00119h.

152. Yi Zhao,Hong Liu,Xun Xi,Shuai Chen,Dongxu Liu
TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT
Exp Cell Res. 2020 Dec 1;397(1):112336.;doi: 10.1016/j.yexcr.2020.112336.

153. Bingbing Zhang,Ming Li,Wei Yang,Juan J Loor,Yusheng Liang,Shuang Wang,Yingying Zhao,Han Guo,Xinru Ma,Liyun Yu,Chuang Xu
Mitochondrial dysfunction and endoplasmic reticulum stress in calf hepatocytes are associated with fatty acid-induced ORAI calcium release-activated calcium modulator 1 signaling
J Dairy Sci. 2020 Dec;103(12):11945-11956.;doi: 10.3168/jds.2020-18684.

154. Jie Wang,Nan Nan,Lulu Shi,Ning Li,Shuangzhan Huang,Ai Zhang,Yutong Liu,Peng Guo,Bao Liu,Zheng-Yi Xu
Arabidopsis BRCA1 represses RRTF1-mediated ROS production and ROS-responsive gene expression under dehydration stress
New Phytol. 2020 Dec;228(5):1591-1610.;doi: 10.1111/nph.16786.

155. Yan Yang,Jie Li,Lingqiang Zhang,Zeyuan Lin,Haiming Xiao,Xiaohong Sun,Meng Zhang,Peiqing Liu,Heqing Huang
CKIP-1 acts downstream to Cx43 on the activation of Nrf2 signaling pathway to protect from renal fibrosis in diabetes
Pharmacol Res. 2021 Jan;163:105333.;doi: 10.1016/j.phrs.2020.105333.

过氧化氢酶检测试剂盒(S0051)

过氧化氢酶检测试剂盒

产品编号: S0051

产品包装:100次
选择包装
100次

说明书下载

458.00 10
价格: ¥ 458.00

产品简介
使用说明
产品图片
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
S0051 过氧化氢酶检测试剂盒 100次 458.00元

过氧化氢酶检测试剂盒(Catalase Assay Kit)是一种简单易行的通过显色反应来检测细胞、组织或其它样品中过氧化氢酶
(Catalase)活性的试剂盒。在过氧化氢相对比较充足的情况下,过氧化氢酶可以催化过氧化氢产生水和氧气。残余的过氧化氢在过氧化物酶(Peroxidase)的催化下可以氧化生色底物,产生红色的产物(N-(4-antipyryl)-3-chloro-5-sulfonate-p-benzoquinonemonoimine),最大吸收波长为520nm。用过氧化氢标准品,制作标准曲线,这样就可以计算出样品中的过氧化氢酶在单位时间单位体积内催化了多少量的过氧化氢转变为水和氧气,从而可以计算出样品中过氧化氢酶的酶活力。
过氧化氢酶分布非常广泛。在肝脏、肾脏和红细胞中,过氧化氢酶的水平非常高,是清除能导致氧化损伤的过氧化氢的主要场所。
过氧化氢酶的活性也可以用紫外分光光度计测定A240,但蛋白质或其它组份在A240附近都有比较强的吸收,会对测定产生严重干扰。因此,用紫外法测定过氧化氢酶的活性,比较适合纯化的过氧化氢酶。本试剂盒通过检测A520来测定过氧化物酶催化下由过氧化氢氧化生色底物产生的红色产物,受干扰的因素小,检测灵敏度高,可以检测出低达1U/ml的过氧化氢酶。
本试剂盒可以检测全血、红细胞裂解产物、血清、组织匀浆产物、细胞裂解产物等生物样品中的过氧化氢酶的活性。一个试剂盒共可以进行100次检测。
包装清单:

产品编号 产品名称 包装
S0051-1 过氧化氢酶检测缓冲液 60ml
S0051-2 过氧化氢 (约1M) 5ml
S0051-3 过氧化氢酶反应终止液 50ml
S0051-4 显色底物 20ml
S0051-5 过氧化物酶 20μl
说明书 1份

保存条件:
-20℃保存,一年有效。 过氧化氢酶检测缓冲液和过氧化氢酶反应终止液也可以4℃保存。
注意事项:
待测的过氧化氢酶样品,无论是纯的过氧化氢酶还是细胞或组织裂解产物,在4℃通常可以保存1周,-70℃可以长期保存,但-20℃保存后过氧化氢酶的活力会显著下降。
在检测过程中加入过氧化氢酶反应终止液后15分钟内需开始显色反应。
过氧化氢不太稳定,精确的过氧化氢浓度需参考本说明书中的方法进行测定。
如需对样品中的过氧化氢酶活力进行精确定量,需自备蛋白浓度测定试剂盒。例如可以选购碧云天生产的BCA蛋白浓度测定试剂盒(P0009/P0010/P0010S/P0011/P0012/P0012S)测定样品的蛋白浓度。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1. 试剂盒的准备工作:
a.  配制250mM过氧化氢溶液。本试剂盒提供的过氧化氢浓度约为1M。由于过氧化氢不是非常稳定,使用前需自行测定过氧化氢的实际浓度。把浓度约为1M的过氧化氢用本试剂盒提供的过氧化氢酶检测缓冲液稀释100倍,使过氧化氢的浓度约为10mM。测定A240。A240的测定可采用如下的任一方法:
(a)普通紫外分光光度计法:使用含比色皿架的紫外分光光度计、NanoDrop 2000C、NanoDrop Oneᶜ、QuickDrop等仪器,配套石英比色皿。确定比色皿光程(path length),一般为1cm。用比色皿检测的过氧化氢浓度最接近实际浓度。
(b)微量紫外分光光度计法:如NanoDrop 2000、NanoDrop One、QuickDrop、含超微量检测板µDrop Plate的Varioskan等仪器。确定光程:对于NanoDrop 2000、NanoDrop One等,需要取消“自动化光程”,此时光程一般为0.1cm;Varioskan的超微量检测板µDrop Plate的光程一般为0.05cm。具体的微量紫外分光光度计的光程请参考仪器参数。
(c)96孔紫外酶标仪法(须能检测240nm波长):根据96孔板的参数确定光程,一般200微升样品的光程为0.552cm (样品体积除以96孔单孔孔内横截面面积)。一般建议使用专用的96孔紫外检测板(如96孔UV板),如果没有紫外检测板,也可使用一般的96孔板,但由于为非紫外检测专用板,会有非常高的紫外吸收信号,所以需要设置含等量双蒸水的孔作为空白对照(一般200μl水在该类96孔板的A240在3.8左右),计算时须减去该空白对照。在使用非紫外检测专用板的情况下,由于96孔酶标仪在240nm的检测上限有限,建议将过氧化氢稀释至约10mM左右后再进行浓度测定。
注意:以上所有方法都需要设置等量双蒸水作为空白对照,并在计算时减去该空白对照。
浓度计算公式:c=A/(ε×b)。其中:c为样品浓度(单位为mol/L或M);A为吸光值;ε为波长依赖的摩尔消光系数(单位为L×mol-1×cm-1或M-1×cm-1),过氧化氢的摩尔消光系数为43.6M-1cm-1;b=光程(单位为cm)。
因此:过氧化氢浓度(M)=A240/(43.6×b);即:过氧化氢浓度(mM)=22.94 × A240/b
从而计算出本试剂盒提供的过氧化氢的实际浓度。
示例:将本试剂盒提供的约为1M的过氧化氢用双蒸水稀释100倍后,用96孔酶标仪及一般的96孔板进行检测,每孔200微升,每组3个平行。双蒸水对照组的平均A240为3.750,过氧化氢样品组的平均A240为3.974,差值为0.224,200微升样品的光程为0.552cm。代入公式,过氧化氢浓度(mM)=22.94×0.224/0.552=9.31,则实际本试剂盒提供的过氧化氢浓度为0.931M。
然后再根据实际的过氧化氢浓度配制250mM过氧化氢溶液。
b.  配制5mM过氧化氢溶液。根据测定得到的实际过氧化氢浓度配制5mM过氧化氢溶液。
c.  配制显色工作液。在冰浴上溶解显色底物,适当分装后再使用,尽量避免反复冻融。其它试剂放置在冰浴上备用。取适当量的过氧化物酶,按照1:1000的比例用显色底物稀释,配制成显色工作液。例如取5μl过氧化物酶,加入5ml显色底物,混匀即得到5ml显色工作液。
2. 样品的准备:
用适当的裂解液裂解细胞或组织(可以使用碧云天生产的Western及IP细胞裂解液(P0013)进行裂解。用本试剂盒提供的过氧化氢酶检测缓冲液稀释样品,裂解好的样品至少加入等体积的过氧化氢酶检测缓冲液进行稀释。具体的稀释倍数可以参考下表。

样品名称 蛋白浓度 检测时的样品使用量 反应时间 A520 (空白对照-样品)
Blank 1.17 (空白对照实测值)
Human red blood cell lysate 0.2mg/ml 4μl 2min 0.46
Rat liver lysate 0.3mg/ml 7.5μl 1min 0.57
Rat kidney lysate 0.3mg/ml 7.5μl 3min 0.42
Rat spleen lysate 0.3mg/ml 7.5μl 3min 0.16
Rat brain lysate 0.3mg/ml 15μl 3min 0.021
HepG2 lysate 2.0mg/ml 3μl 3min 0.32
Jurkat lysate 2.0mg/ml 3μl 3min 0.46

说明:表中的数据仅供参考,实际测定值由于检测体系的不同等可能与参考值之间存在20-50%的误差。对于相同的样品,同样的实验体系,测得的A520值和表1相比可能会有较大差异。如果实验体系不同,例如测得蛋白浓度的方法不同,使用的检测波长不同等,测得的A520值和我们提供的参考数值相比可能会有更大的差异。对于表中没有列出的样品,可以参考表中的类似样品进行检测,然后再根据检测结果适当调整蛋白浓度和样品使用量,或反应时间。通常对于完全未知的样品,可以对样品进行1、10、20和50倍稀释,然后各取10μl,反应1分钟。样品稀释后的浓度,最好可以使反应体系中的过氧化氢在反应1-5分钟后减少30-50%左右,这时的检测结果更加精确。
a.  细胞样品的准备。可以用碧云天生产的Western及IP细胞裂解液(P0013)参考相应的说明裂解细胞样品。
b.  组织样品的准备。可以用碧云天生产的Western及IP细胞裂解液(P0013)参考相应的说明裂解组织样品。
c.  全血样品的准备。收集全血(whole blood)至一抗凝管内,颠倒混匀。取100微升全血冻融一次,用过氧化氢酶检测缓冲液稀释1000倍后进行后续检测。
d.  红细胞裂解液的准备。用抗凝管收集血液,颠倒混匀。取至少500微升全血4℃ 2500g离心5分钟。弃上清,沉淀用冰冷的生理盐水(0.9%氯化钠)洗涤3次。用约5倍细胞体积的冰冷的去离子水,例如Milli-Q纯水,重悬细胞沉淀,冰浴10分钟。使用前用过氧化氢酶检测缓冲液稀释400倍后进行后续检测。
3. 标准曲线测定:
a.  取0、12.5、25、50或75微升配制好的5mM过氧化氢溶液至1.5ml或0.5ml塑料离心管中,分别加入过氧化氢酶检测缓冲液至最后体积为100微升,混匀,此时过氧化氢溶液浓度分别为0、0.625、1.25、2.5、3.75mM。如有需要,可以设置更高浓度的过氧化氢标准溶液。
b.  各取4微升,加入到96孔板中的一个孔内。加入200μl显色工作液。25℃至少孵育15分钟后测定A520,但孵育时间不宜超过45分钟。(注:本步骤可以和样品测定步骤中的最后一步同时进行。)
4. 样品测定:

空白对照 (blank) 样品 (sample)
样品体积 0 μl x μl
过氧化氢酶检测缓冲液 40 μl 40-x μl
250mM过氧化氢溶液 10 μl 10 μl

a.  参考上表,取x微升(0-40微升)样品至1.5ml塑料离心管中,加入过氧化氢酶检测缓冲液至体积为40微升(即加入40-x微升过氧化氢酶检测缓冲液),混匀。再加入10微升250mM过氧化氢溶液,用移液器迅速混匀。参考表1,25℃反应1-5分钟。
b.  加入450微升过氧化氢酶反应终止液,颠倒混匀或Vortex混匀以终止反应。需在终止反应后15分钟内完成下面的步骤c和步骤d。
c.  在一洁净的塑料离心管内加入40微升过氧化氢酶检测缓冲液,再加入10微升已终止并混匀的上述反应体系,混匀。
d.  从上一步骤的50微升体系中取10微升加入到96孔板中的一个孔内。加入200微升显色工作液。
e.  25℃至少孵育15分钟后测定A520,但孵育时间不宜超过45分钟。
5. 样品中过氧化氢酶酶活力的计算:
a.  计算出标准曲线。A520=k[过氧化氢微摩尔数]+b,由标准曲线计算出k和b的值。标准曲线的检测效果请参考图1。
例如本说明书中的标准曲线公式为:y=70.281x +0.06,即k=70.281,b=0.06,则A520=70.281 × [过氧化氢微摩尔数]+0.06。

过氧化氢酶检测试剂盒(S0051)

图1. 本过氧化氢酶检测试剂盒的标准曲线效果图。配制好的过氧化氢标准溶液(0、0.625、1.25、2.5、3.75、5mM)各取4微升,用本试剂盒进行检测,对应的微摩尔数分别为0、0.0025、0.005、0.01、0.015、0.02微摩尔。不同的检测条件下,实际读数会因检测仪器等的不同而存在差异,图中数据仅供参考。

b.  计算出样品中残余的过氧化氢微摩尔数。
残余过氧化氢微摩尔数=(A520-b)/k
c.  过氧化氢酶酶活力单位的定义:1个酶活力单位(1 unit)在25℃,pH7.0的条件下,在1分钟内可以催化分解1微摩尔过氧化氢。
d1. 对于细胞或组织样品的过氧化氢酶活力计算:
[样品过氧化氢酶酶活力]=[消耗过氧化氢微摩尔数] × [稀释倍数]/([反应分钟数] × [样品体积] × [蛋白浓度])
[样品过氧化氢酶酶活力]的单位为units/mg蛋白
[消耗过氧化氢微摩尔数]=[空白对照残余过氧化氢微摩尔数]-[样品残余过氧化氢微摩尔数]
[稀释倍数]=250
[反应分钟数]即为实际的反应分钟数
[样品体积]为表2中的X微升,以毫升来表示即为X/1000毫升。
[蛋白浓度]为取X微升样品时,样品中的蛋白浓度,单位为mg/ml。
d2. 对于血浆等液体样品的过氧化氢酶活力计算:
[样品过氧化氢酶酶活力]=[消耗过氧化氢微摩尔数] X[稀释倍数]/([反应分钟数]X[样品体积])
[样品过氧化氢酶酶活力]的单位为units/ml样品

过氧化氢酶检测试剂盒(S0051)

相关产品请点击如下按钮:
活性氧相关

使用本产品的文献:
1. Wang DW, Hu YL, Liu YJ, Lin ZP.
Studies on the Function of Vitreoscilla Hemoglobin Gene in Improving Sur factin Producing Rate of Bacillus subtilis.
BIOTECHNOL LETT . 2007;5:116-9. (IF 1.977)

2. He Z, Sun X, Mei G, Yu S, Li N.
Nonclassical secretion of human catalase on the surface of CHO cells is more efficient than classical secretion.
Cell Biol Int . 2008 Apr;32(4):367-73. (IF 2.571)

3. He Z, Yu S, Mei G, Zheng M, Wang M, Dai Y, Tang B, Li N.
Maternally transmitted milk containing recombinant human catalase provides protection against oxidation for mouse offspring during lactation.
FREE RADICAL BIO MED . 2008 Oct 15;45(8):1135-42. (IF 6.17)

4. Zhu M, Gu F, Shi J, Hu J, Hu Y, Zhao Z.
Increased oxidative stress and astrogliosis responses in conditional double-knockout mice of Alzheimer-like presenilin-1 and presenilin-2.
FREE RADICAL BIO MED . 2008 Nov 15;45(10):1493-9. (IF 6.17)

5. Wang XZ, Liu SS, Sun Y, Wu JY, Zhou YL, Zhang JH.
Beta-cypermethrin impairs reproductive function in male mice by inducing oxidative stress.
Theriogenology . 2009 Sep 15;72(5):599-611. (IF 2.094)

6. Qian J, Jiang F, Wang B, Yu Y, Zhang X, Yin Z, Liu C.
Ophiopogonin D prevents H2O2-induced injury in primary human umbilical vein endothelial cells.
J Ethnopharmacol . 2010;128(2):438-45. (IF 3.69)

7. Huang X, Zhuang J, Teng X, Li L, Chen D, Yan X, Tang F.
The promotion of human malignant melanoma growth by mesoporous silica nanoparticles through decreased reactive oxygen species.
Biomaterials . 2010;31(24):6142-53. (IF 10.317)

8. Chen Y, Chang L, Li W, Rong Z, Liu W, Shan R, Pan R.
Thioredoxin protects fetal type II epithelial cells from hyperoxia-induced injury.
PEDIATR PULM . 2010 Dec;45(12):1192-200. (IF 2.534)

9. Li W, Wu Y, Ren C, Lu Y, Gao Y, Zheng X, Zhang C.
The activity of recombinant human neuroglobin as an antioxidant and free radical scavenger.
Proteins . 2011 Jan;79(1):115-25. (IF 2.828)

10. Fan J, Yin JJ, Ning B, Wu X, Hu Y, Ferrari M, Anderson GJ, Wei J, Zhao Y, Nie G.
Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles.
Biomaterials . 2011 Feb;32(6):1611-8. (IF 10.317)

11. Wang J, Sun P, Bao Y, Liu J, An L.
Cytotoxicity of single-walled carbon nanotubes on PC12 cells.
Toxicol In Vitro . 2011 Feb;25(1):242-50. (IF 2.959)

12. Yang L, Zheng XL, Sun H, Zhong YJ, Wang Q, He HN, Shi XW, Zhou B, Li JK, Lin Y, Zhang L, Wang X.
Catalase suppression-mediated H(2)O(2) accumulation in cancer cells by wogonin effectively blocks tumor necrosis factor-induced NF-κB activation and sensitizes apoptosis.
Cancer Sci . 2011 Apr;102(4):870-6. (IF 4.966)

13. Liu XM, Zhou Q, Xu SZ, Wakamatsu K, Lei TC.
Maintenance of immune hyporesponsiveness to melanosomal proteins by DHICA-mediated antioxidation: Possible implications for autoimmune vitiligo.
FREE RADICAL BIO MED . 2011 May 1;50(9):1177-85. (IF 6.17)

14. Min L, He S, Chen Q, Peng F, Peng H, Xie M.
Comparative proteomic analysis of cellular response of human airway epithelial cells (A549) tobenzo(a)pyrene.
TOXICOL MECH METHOD . 2011 Jun;21(5):374-82. (IF 2.295)

15. Wang S, Duan Y, Zeng Q, Zhao Z, Wang X.
[D-Ala2, D-Leu5]-Enkephalin (Dadle) Reduces Inflammation Responses after Transient Global Ischemia.
ADV MATER . 2011 Sep;345:343-48. (IF 27.398)

16. Shi X, Gu A, Ji G, Li Y, Di J, Jin J, Hu F, Long Y, Xia Y, Lu C, Song L, Wang S, Wang X.
Developmental toxicity of cypermethrin in embryo-larval stages of zebrafish.
Chemosphere . 2011 Oct;85(6):1010-6. (IF 5.778)

17. Zhou J, Li Y, Yan G, Bu Q, Lv L, Yang Y, Zhao J, Shao X, Deng Y, Zhu R, Zhao Y, Cen X.
Protective role of taurine against morphine-induced neurotoxicity in C6 cells via inhibition ofoxidative stress.
Neurotox Res . 2011 Nov;20(4):334-42. (IF 2.992)

18. Zhang Y, Fu D, Yu F, Liu Q, Yu Z.
Two catalase homologs are involved in host protection against bacterial infection and oxidative stress in Crassostrea hongkongensis.
FISH SHELLFISH IMMUN . 2011 Dec;31(6):894-903. (IF 3.298)

19. Cao L, Dai C, Li Z, Fan Z, Song Y, Wu Y, Cao Z, Li W.
Antibacterial activity and mechanism of a scorpion venom Peptide derivative in vitro and in vivo.
PLoS One . 2012;7(7):e40135. (IF 2.74)

20. Tie L, Yang HQ, An Y, Liu SQ, Han J, Xu Y, Hu M, Li WD, Chen AF, Lin ZB, Li XJ.
Ganoderma lucidum polysaccharide accelerates refractory wound healing by inhibition of mitochondrial oxidativestress in type 1 diabetes.
CELL PHYSIOL BIOCHEM . 2012;29(3-4):583-94. (IF 5.5)

21. Shi H, Wang Y, Cheng Z, Ye T, Chan Z.
Analysis of natural variation in bermudagrass (Cynodon dactylon) reveals physiological responses underlyingdrought tolerance.
PLoS One . 2012;7(12):e53422. (IF 2.74)

22. Zhao G, Yao-Yue C, Qin GW, Guo LH.
Luteolin from Purple Perilla mitigates ROS insult particularly in primary neurons.
Neurobiol Aging . 2012 Jan;33(1):176-86. (IF 4.347)

23. Li NS, Luo XJ, Dai Z, Liu B, Zhang YS, Yang ZC, Peng J.
Beneficial effects of capsiate on ethanol-induced mucosal injury in rats are related to stimulationof calcitonin gene-related Peptide release.
Planta Med . 2012 Jan;78(1):24-30. (IF 2.687)

24. Liu JH, Qi ZM, Huang Q, Wei XL,Ke ZG, Fang YS, Tian YC, Yu ZG.
Study of energetic-particle-irradiation induced biological effect on Rhizopus oryzae through Synchrotron-FTIR micro-spectroscopy.
Journal of Molecular Structure . 2012 Jan;1031(16):1-8. (IF 2.12)

25. Wang J, Sun P, Bao Y, Dou B, Song D, Li Y.
Vitamin E renders protection to PC12 cells against oxidative damage and apoptosis induced bysingle-walled carbon nanotubes.
Toxicol In Vitro . 2012 Feb;26(1):32-41. (IF 2.959)

26. Wang H, Meng XL, Xu JP, Wang J, Wang H, Ma CW.
Production, purification, and characterization of the cecropin from Plutella xylostella, pxCECA1using an intein-induced self-cleavable system in Escherichia coli.
APPL MICROBIOL BIOT . 2012 May;94(4):1031-9. (IF 3.53)

27. Shao X, Hu Z, Hu C, Bu Q, Yan G, Deng P, Lv L, Wu D, Deng Y, Zhao J, Zhu R, Li Y, Li H, Xu Y, Yang H, Zhao Y, Cen X.
Taurine protects methamphetamine-induced developmental angiogenesis defect throughantioxidant mechanism.
TOXICOL APPL PHARM  . 2012 May 1;260(3):260-70. (IF 3.616)

28. Sun WH, Liu F, Chen Y, Zhu YC.
Hydrogen sulfide decreases the levels of ROS by inhibiting mitochondrial complex IV andincreasing SOD activities in cardiomyocytes under ischemia/reperfusion.
BIOCHEM BIOPH RES CO . 2012 May 4;421(2):164-9. (IF 2.985)

29. Deng X, Wu K, Wan J, Li L, Jiang R, Jia M, Jing Y, Zhang L.
Aminotriazole attenuated carbon tetrachloride-induced oxidative liver injury in mice.
Food Chem Toxicol . 2012 Jun 8;50(9):3073-8. (IF 4.679)

30. Li XC, Xing YZ, Jiang X, Qiao J,Tan HL, Zhou B.
Identification And Characterization Of The Catalase Gene Pycat From The Red Alga Pyropia Yezoensis (Bangiales, Rhodophyta) 1.
Journal of Phycology . 2012 Jun;48(3):664-9. (IF 2.831)

31. Liu Z, Li S, Cai Y, Wang A, He Q, Zheng C, Zhao T, Ding X, Zhou X.
Manganese superoxide dismutase induces migration and invasion of tongue squamous cell carcinoma via H2O2-dependent Snail signaling.
FREE RADICAL BIO MED . 2012 Jul 1;53(1):44-50. (IF 6.17)

32. Zhou T, Zong R, Zhang Z, Zhu C, Pan F, Xiao X, Liu Z, He H, Ma JX, Liu Z, Zhou Y.
SERPINA3K Protects against Oxidative Stress via Modulating ROS Generation/Degradation andKEAP1-NRF2 Pathway in the Corneal Epithelium.
INVEST OPHTH VIS SCI . 2012 Jul 27;53(8):5033-43. (IF 3.47)

33. Cao L, Li Z, Zhang R, Wu Y, Li W, Cao Z.
StCT2, a new antibacterial peptide characterized from the venom of the scorpion Scorpiopstibetanus.
Peptides . 2012 Aug;36(2):213-20. (IF 2.843)

34. Zheng J, Yang B, Yu Y, Chen Q, Huang T, Li D.
Ganoderma lucidum polysaccharides exert anti-hyperglycemic effect on streptozotocin-induced diabetic rats through affecting β-cells.
COMB CHEM HIGH T SCR . 2012 Aug;15(7):542-50. (IF 1.195)

35. Wang X, Zhang Y, Li T, Tian W, Zhang Q, Cheng Y.
Generation 9 polyamidoamine dendrimer encapsulated platinum nanoparticle mimics catalase size, shape, andcatalytic activity.
Langmuir . 2013 Apr 30;29(17):5262-70. (IF 3.557)

36. Shi H, Ye T, Wang Y, Chan Z.
Arabidopsis ALTERED MERISTEM PROGRAM 1 negatively modulates plant responses to abscisic acid anddehydration stress.
PLANT PHYSIOL BIOCH . 2013 Apr 3;67C:209-216. (IF 3.72)

37. Ren C, Li Y, Han R, Gao D, Li W, Shi J, Hoogewijs D, Braeckman BP, De Henau S, Lu Y, Qu W, Gao Y, Wu Y, Li Z, Liu H, Wang Z, Zhang C.
GLB-13 is associated with oxidative stress resistance in caenorhabditis elegans.
IUBMB Life . 2013 May;65(5):423-34. (IF 3.244)

38. Zhang H, Shao D, Wu Y, Dai B, Cai C, Fang W, Ye B, Zhang Y, liu J, Jia X.
Regulation of nodularin-induced apoptosis by epigallocatechin-3-gallate on fish lymphocytes in vitro.
FISH SHELLFISH IMMUN . 2013 May;34(5):1085-93. (IF 3.298)

39. Wang M, Wang L, Zhou Z, Gao Y, Wang L, Shi X, Gai Y, Mu C, Song L.
The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis.
Int J Immunogenet . 2013 Jun;40(3):230-40. (IF 1.13)

40. Sun P, Chen JY, Li J, Sun MR, Mo WC, Liu KL, Meng YY, Liu Y, Wang F, He RQ, Hua Q.
The protective effect of geniposide on human neuroblastoma cells in the presence of formaldehyde.
BMC COMPLEM ALTERN M . 2013 Jul 1;13:152. (IF 2.833)

41. Yu CH, Liu ZY, Sun W, Li YJ, Zhang DS, Pan RT, Sun ZL.
Effect of Danofloxacin on Reactive Oxygen Species Production, Lipid Peroxidation and Antioxidant EnzymeActivities in Kidney Tubular Epithelial Cell Line, LLC-PK1.
BASIC CLIN PHARMACOL . 2013 Dec;113(6):377-84. (IF 2.651)

42. He Z, Zhang X, Chen C, Wen Z, Hoopes SL, Zeldin DC, Wang DW.
Cardiomyocyte-specific expression of CYP2J2 prevents development of cardiac remodelling induced by angiotensin II.
Cardiovasc Res . 2015 Mar 1;105(3):304-17. (IF 8.168)

43. Luo C, Li Y, Yang L, Wang X, Long J, Liu J.
Superparamagnetic iron oxide nanoparticles exacerbate the risks of reactive oxygen species-mediated external stresses.
Arch Toxicol . 2015 Mar;89(3):357-69. (IF 5.059)

44. Hu Y, Li R, Yang H, Luo H, Chen Z.
Sirtuin 6 is essential for sodium sulfide-mediated cytoprotective effect in ischemia/reperfusion-stimulated brain endothelial cells.
J STROKE CEREBROVASC  . 2015 Mar;24(3):601-9. (IF 1.598)

45. Chen L, Bi B, Zeng J, Zhou Y, Yang P, Guo Y, Zhu J, Yang Q, Zhu N, Liu T.
Rosiglitazone ameliorates senescence-like phenotypes in a cellular photoaging model.
J Dermatol Sci . 2015 Mar;77(3):173-81. (IF 3.681)

46. Su H, Liu DD, Zhao M, Hu WL, Xue SS, Cao Q, Le XY, Ji LN, Mao ZW.
Dual-Enzyme Characteristics of Polyvinylpyrrolidone-Capped Iridium Nanoparticles and Their Cellular Protective Effect against H2O2-Induced Oxidative Damage.
ACS APPL MATER INTER . 2015 Apr 22;7(15):8233-42. (IF 8.758)

47. Cai W, Liu W, Wang WS, Fu ZW, Han TT, Lu YT.
Overexpression of Rat Neurons Nitric Oxide Synthase in Rice Enhances Drought and Salt Tolerance.
PLoS One . 2015 Jun 29;10(6):e0131599. (IF 2.74)

48. Keogh K, Waters SM, Kelly AK, Wylie AR, Kenny DA.
Effect offeed restriction and subsequent re-alimentation on hormones and genes of the somatotropic axis in cattle.
Physiol Genomics . 2015 Jul;47(7):264-73. (IF 2.749)

49. Chen Z, Wang D, Gu C, Liu X, Pei W, Li J, Cao Y, Jiao Y, Tong J, Nie J.
Down-regulation of let-7 microRNA increased K-ras expression in lung damage induced by radon.
ENVIRON TOXICOL CHEM . 2015 Sep;40(2):541-8. (IF 3.152)

50. Dou T, Yan M, Wang X, Lu W, Zhao L, Lou D, Wu C, Chang X, Zhou Z.
Nrf2/ARE Pathway Involved in Oxidative Stress Induced by Paraquat in Human Neural Progenitor Cells.
Oxid Med Cell Longev . 2016;2016:8923860. (IF 5.076)

51. Yao C, Li G, Qian Y, Cai M, Yin H, Xiao L, Tang W, Guo F, Shi B.
Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia.
Oxid Med Cell Longev . 2016;2016:3406802. (IF 5.076)

52. Sun H, Li G, An T, Zhao H, Wong PK.
Unveiling the photoelectrocatalytic inactivation mechanism of Escherichia coli: Convincing evidence from responses of parent and anti-oxidation single gene knockout mutants.
Water Res . 2016 Jan 1;88:135-43. (IF 9.13)

53. Jiao Y, Ma S, Wang Y, Li J, Shan L, Liu Q, Liu Y, Song Q, Yu F, Yu H, Liu H, Huang L, Chen J.
N-Acetyl Cysteine Depletes Reactive Oxygen Species and Prevents Dental Monomer-Induced IntrinsicMitochondrial Apoptosis In Vitro in Human Dental Pulp Cells.
PLoS One . 2016 Jan 25;11(1):e0147858. (IF 2.74)

54. Liu Z, Gan L, Chen Y, Luo D, Zhang Z, Cao W, Zhou Z, Lin X, Sun C.
Mark4 promotes oxidative stress and inflammation via binding to PPARγ and activating
SCI REP-UK . 2016 Feb 18;6:21382. (IF 3.998)

55. Chen W, Su H, Xu Y, Bao T, Zheng X.
Protective effect of wild raspberry (Rubus hirsutus Thunb.) extract against acrylamide-induced oxidative damage is potentiated after simulated gastrointestinal digestion.
Food Chem . 2016 Apr 1;196:943-52. (IF 6.306)

56. Lin J, Zou Y, Cao K, Ma C, Chen Z.
The impact of heterologous catalase expression and superoxide dismutase overexpression on enhancingthe oxidative resistance in Lactobacillus casei.
J IND MICROBIOL BIOT . 2016 May;43(5):703-11. (IF 2.824)

57. Huang H, Xiao X, Lin F, Grossart HP, Nie Z, Sun L, Xu C, Shi J.
Continuous-release beads of natural allelochemicals for the long-term control of cyanobacterial growth:Preparation, release dynamics and inhibitory effects.
Water Res . 2016 May 15;95:113-23. (IF 9.13)

58. Wang J, Chen X, Wang F, Zhang J, Li P, Li Z, Xu J, Gao F, Jin C, Tian H, Zhang J, Li W, Lu L, Xu GT.
OFD1, as a Ciliary Protein, Exhibits Neuroprotective Function in Photoreceptor Degeneration Models.
PLoS One . 2016 May 19;11(5):e0155860. (IF 2.74)

59. Wang M, Zhao X, Xiao Z, Yin X, Xing T, Xia G.
A wheat superoxide dismutase gene TaSOD2 enhances salt resistance through modulating redoxhomeostasis by promoting NADPH oxidase activity.
Plant Mol Biol . 2016 May;91(1-2):115-30. (IF 3.302)

60. Ran B, Li M, Li Y, Lin Y, Liu W, Luo Q, Fu Y, Tang Q, Yang Y, Pu Y.
Everolimus (RAD001) inhibits the proliferation of rat vascular smooth muscle cells by up-regulating theactivity of the p27/kip1 gene promoter.
Anatol J Cardiol . 2016 Jun;16(6):385-91. (IF 1.223)

61. Dong Y, Cui P, Li Z, Zhang S.
Aging asymmetry: systematic survey of changes in age-related biomarkers in the annual fishNothobranchius guentheri.
Fish Physiol Biochem . 2016 Sep 10.[Epub ahead of print] (IF 2.242)

62. Meng J, Lv Z, Qiao X, Li X, Li Y, Zhang Y, Chen C.
The decay of Redox-stress Response Capacity is a substantive characteristic of aging: Revising the redoxtheory of aging.
Redox Biol . 2016 Dec 28;11:365-374. (IF 9.986)

63. Han Y, Su J, Liu X, Zhao Y, Wang C, Li X.
Naringin alleviates early brain injury after experimental subarachnoid hemorrhage by reducing oxidativestress and inhibiting apoptosis.
Brain Res Bull . 2016 Dec 21. pii:S0361-9230(16)30479-8. (IF 3.37)

64. Chen YQ, Liu XG, Zhao W, Cui H, Ruan J, Yuan Y, Tu Z.
MET18 Deficiency Increases the Sensitivity of Yeast to Oxidative Stress and Shortens ReplicativeLifespan by Inhibiting Catalase Activity.
Biomed Res Int . 2017;2017:7587395. (IF 2.276)

65. Hou B, Qiang G, Zhao Y, Yang X, Chen X, Yan Y, Wang X, Liu C, Zhang L, Du G.
Salvianolic Acid A Protects Against Diabetic Nephropathy through Ameliorating GlomerularEndothelial Dysfunction via Inhibiting AGE-RAGE Signaling.
CELL PHYSIOL BIOCHEM . 2017;44(6):2378-2394. (IF 5.5)

66. Chen W, Su H, Xu Y, Jin C.
In vitro gastrointestinal digestion promotes the protective effect of blackberry extract against acrylamide-induced oxidative stress.
SCI REP-UK . 2017 Jan 13;7:40514. (IF 3.998)

67. Chen W, Su H, Xu Y, Jin C.
In vitro gastrointestinal digestion promotes the protective effect of blackberry extract againstacrylamide-induced oxidative stress.
SCI REP-UK . 2017 Jan 13;7:40514. (IF 3.998)

68. Xuanfei L, Hao C, Zhujun Y, Yanming L, Jianping G.
Imidazoline I2 receptor inhibitor idazoxan regulates the progression of hepatic fibrosis via Akt-Nrf2-Smad2/3 signaling pathway.
ONCOTARGET . 2017 Mar 28;8(13):21015-21030. (IF 5.168)

69. Wan B, Zhang Q, Ni J, Li S, Wen D, Li J, Xiao H, He P, Ou HY, Tao J, Teng Q, Lu J, Wu W, Yao YF.
Type VI secretion system contributes to Enterohemorrhagic Escherichia coli virulence by secreting catalase against host reactive oxygen species (ROS).
PLoS Pathog . 2017 Mar 13;13(3):e1006246. (IF 6.218)

70. Dong Y, Cui P, Li Z, Zhang S.
Aging asymmetry: systematic survey of changes in age-related biomarkers inthe annual fishNothobranchius guentheri.
Fish Physiol Biochem . 2017 Apr;43(2):309-319. (IF 2.242)

71. Zhu Z, Fan X, Pan Y, Lu Y, Zeng W.
Trehalose improves rabbit sperm quality during cryopreservation.
Cryobiology . 2017 Apr;75:45-51. (IF 2.283)

72. Meng J, Lv Z, Qiao X, Li X, Li Y, Zhang Y, Chen C.
The decay of Redox-stress Response Capacity is a substantive characteristic of aging: Revising the redox theory of aging.
Redox Biol . 2017 Apr;11:365-374. (IF 9.986)

73. Han X, Xue X, Zhao Y, Li Y, Liu W, Zhang J, Fan S.
Rutin-Enriched Extract from Coriandrum sativum L. Ameliorates Ionizing Radiation-InducedHematopoietic Injury.
Int J Mol Sci . 2017 Apr 29;18(5). pii: E942. (IF 4.556)

74. Zhang GM, Deng MT, Lei ZH, Wan YJ, Nie HT, Wang ZY, Fan YX, Wang F, Zhang YL.
Effects of NRF1 on steroidogenesis and apoptosis in goat luteinized granulosa cells.
Reproduction . 2017 Aug;154(2):111-122. (IF 3.206)

75. Zhang L, Xu Y, Li Y, Bao T, Gowd V, Chen W.
Protective property of mulberry digest against oxidative stress-A potential approach to ameliorate dietary acrylamide-induced cytotoxicity.
Food Chem . 2017 Sep 1;230:306-315. (IF 6.306)

76. Feng M, Yin H, Peng H, Liu Z, Lu G, Dang Z.
Hexavalent chromium induced oxidative stress and apoptosis in Pycnoporus sanguineus.
Environ Pollut . 2017 Sep;228:128-139. (IF 6.792)

77. Gao Y, Zhang J, Liu Y, Zhang S, Wang Y, Liu B, Liu H, Li R, Lv C, Song X.
Regulation of TERRA on telomeric and mitochondrial functions in IPF pathogenesis.
BMC Pulm Med . 2017 Dec 2;17(1):163. (IF 2.813)

78. Wang M, Wang L, Jia Z, Wang X, Yi Q, Zhao L, Song L.
The versatile functions of LRR-only proteins in mollusk Chlamys farreri.
Dev Comp Immunol . 2017 Dec;77:188-199. (IF 3.192)

79. Zhang S,Huang Q,Wang Q,Wang Q,Cao X,Zhao L,Xu N,Zhuge Z,Mao J,Fu X,Liu R,Wilcox CS,Patzak A,Li L,Lai EY
Enhanced Renal Afferent Arteriolar Reactive Oxygen Species and Contractility to Endothelin-1 Are Associated with Canonical Wnt Signaling in Diabetic Mice.
KIDNEY BLOOD PRESS R . 2018;43(3):860-871. (IF 1.898)

80. Tao J, Xu J, Chen F, Xu B, Gao J, Hu Y.
Folate acid-Cyclodextrin/Docetaxel induces apoptosis in KB cells via the intrinsic mitochondrialpathway and displays antitumor activity in vivo.
Eur J Pharm Sci . 2018 Jan 1;111:540-548. (IF 3.616)

81. Dai J,Gu L,Su Y,Wang Q,Zhao Y,Chen X,Deng H,Li W,Wang G,Li K
Inhibition of curcumin on influenza A virus infection and influenzal pneumonia via oxidative stress, TLR2/4, p38/JNK MAPK and NF-κB pathways.
Int Immunopharmacol . 2018 Jan;54:177-187. (IF 3.943)

82. Wu H,Li F,Wang S,Lu J,Li J,Du Y,Sun X,Chen X,Gao J,Ling D
Ceria nanocrystals decorated mesoporous silica nanoparticle based ROS-scavenging tissue adhesive for highly efficient regenerative wound healing.
Biomaterials. 2018 Jan; (IF 10.317)

83. Tao J,Xu J,Chen F,Xu B,Gao J,Hu Y
Folate acid-Cyclodextrin/Docetaxel induces apoptosis in KB cells via the intrinsic mitochondrial pathway and displays antitumor activity in vivo.
Eur J Pharm Sci. 2018 Jan 1; (IF 3.616)

84. Wang L,Wang F,Liu S,Yang X,Yang J,Ming D
VEGF attenuates 2-VO induced cognitive impairment and neuronal injury associated with the activation of PI3K/Akt and Notch1 pathway.
Exp Gerontol . 2018 Feb;102:93-100. (IF 3.376)

85. Wang N, Zhang J, Qin M, Yi W, Yu S, Chen Y, Guan J, Zhang R.
Amelioration of streptozotocin induced pancreatic β cell damage by morin: Involvement of the AMPK FOXO3 catalase signaling pathway.
Int J Mol Med . 2018 Mar;41(3):1409-1418. (IF 3.098)

86. Wang J, Zhang J, Chen X, Yang Y, Wang F, Li W, Awuti M, Sun Y, Lian C, Li Z, Wang M, Xu JY, Jin C, Tian H, Gao F, Zhang J, Sinha D, Lu L, Xu GT.
miR-365 promotes diabetic retinopathy through inhibiting Timp3 and increasing oxidative stress.
Exp Eye Res . 2018 Mar;168:89-99. (IF 3.011)

87. Wang N,Zhang J,Qin M,Yi W,Yu S,Chen Y,Guan J,Zhang R
Amelioration of streptozotocin‑induced pancreatic β cell damage by morin: Involvement of the AMPK‑FOXO3‑catalase signaling pathway.
Int J Mol Med. 2018 Mar; (IF 3.098)

88. Zang Y,Liu J,Tang XX,Zhou B
Description of a Zostera marina catalase gene involved in responses to temperature stress.
PeerJ. 2018 Mar 26; (IF 2.379)

89. Ren C,Hu X,Zhou Q
Graphene Oxide Quantum Dots Reduce Oxidative Stress and Inhibit Neurotoxicity In Vitro and In Vivo through Catalase-Like Activity and Metabolic Regulation.
Adv Sci (Weinh). 2018 Mar 4;

90. Lu QB, Wan MY, Wang PY, Zhang CX, Xu DY, Liao X, Sun HJ.
Chicoric acid prevents PDGF-BB-induced VSMC dedifferentiation, proliferation and migration by suppressing ROS/NFκB/mTOR/P70S6K signaling cascade.
Redox Biol . 2018 Apr;14:656-668. (IF 9.986)

91. Zhao L, Qi L, Li C, Li L, Jin L, Yuan J.
SVCV impairs mitochondria complex Ⅲ resulting in accumulation of hydrogen peroxide.
FISH SHELLFISH IMMUN . 2018 Apr;75:58-65. (IF 3.298)

92. Ma TJ,Lan DH,He SZ,Ye Z,Li P,Zhai W,Chen WQ,Huang Y,Fu Y,Sun A,Wang YB,Ye Z,Li JL,Gao Y,Yan XL,Li ZH
Nrf2 protects human lens epithelial cells against H2O2-induced oxidative and ER stress: The ATF4 may be involved.
Exp Eye Res . 2018 Apr;169:28-37. (IF 3.011)

93. Feng M, Yin H, Cao Y, Peng H, Lu G, Liu Z, Dang Z.
Cadmium-induced stress response of Phanerochaete chrysosporium duringthe biodegradation of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47).
ECOTOX ENVIRON SAFE . 2018 Jun 15;154:45-51. (IF 4.872)

94. Chen Y,Wang B,Zhao H
Thymoquinone reduces spinal cord injury by inhibiting inflammatory response, oxidative stress and apoptosis via PPAR-γ and PI3K/Akt pathways.
Exp Ther Med. 2018 Jun; (IF 1.785)

95. Dong Z,Yuan Y
Accelerated inflammation and oxidative stress induced by LPS in acute lung injury: Ιnhibition by ST1926.
Int J Mol Med. 2018 Jun; (IF 3.098)

96. Zhang X,Li J,Cheng Y,Yi J,Liu X,Cheng W
Downregulation of CUEDC2 prevents doxorubicin‑induced cardiotoxicity in H9c2 cells.
Mol Med Rep. 2018 Jul; (IF 2.1)

97. Su T,Wang P,Li H,Zhao Y,Lu Y,Dai P,Ren T,Wang X,Li X,Shao Q,Zhao D,Zhao Y,Ma C
The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome-derived signaling in plant development.
J Integr Plant Biol. 2018 Jul; (IF 4.885)

98. Zou MZ,Liu WL,Li CX,Zheng DW,Zeng JY,Gao F,Ye JJ,Zhang XZ
A Multifunctional Biomimetic Nanoplatform for Relieving Hypoxia to Enhance Chemotherapy and Inhibit the PD-1/PD-L1 Axis.
Small. 2018 Jul; (IF 11.459)

99. Wang Y,Chen G,Yan J,Chen X,He F,Zhu C,Zhang J,Lin J,Pan G,Yu J,Pei M,Yang H,Liu T
Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells.
Oxid Med Cell Longev. 2018 Jul 15; (IF 5.076)

100. Ding W,Zhao Y,Xu JW,Zhao P,Li T,Ma H,Reiter RJ,Yu X
Melatonin: A Multifunctional Molecule That Triggers Defense Responses against High Light and Nitrogen Starvation Stress in Haematococcus pluvialis.
J AGR FOOD CHEM. 2018 Jul 25; (IF 4.192)

101. Xue J,Gu H,Liu D,Ma W,Wei X,Zhao L,Liu Y,Zhang C,Yuan Z
Mitochondrial dysfunction is implicated in retinoic acid-induced spina bifida aperta in rat fetuses.
Int J Dev Neurosci . 2018 Aug;68:39-44. (IF 1.911)

102. Chen L,Zhang J,Li C,Wang Z,Li J,Zhao D,Wang S,Zhang H,Huang Y,Guo X
Glycine Transporter-1 and glycine receptor mediate the antioxidant effect of glycine in diabetic rat islets and INS-1 cells.
FREE RADICAL BIO MED . 2018 Aug 1;123:53-61. (IF 6.17)

103. Fu Y,Wu HQ,Cui HL,Li YY,Li CZ
Gastroprotective and anti-ulcer effects of oxymatrine against several gastric ulcer models in rats: Possible roles of antioxidant, antiinflammatory, and prosurvival mechanisms.
Phytother Res. 2018 Oct; (IF 4.087)

104. Wang XX,Zhang TY,Dao GH,Hu HY
Tolerance and resistance characteristics of microalgae Scenedesmus sp. LX1 to methylisothiazolinone.
Environ Pollut. 2018 Oct; (IF 6.792)

105. Li M,Wang S,Li X,Jiang L,Wang X,Kou R,Wang Q,Xu L,Zhao N,Xie K
Diallyl sulfide protects against lipopolysaccharide/d-galactosamine-induced acute liver injury by inhibiting oxidative stress, inflammation and apoptosis in mice.
Food Chem Toxicol. 2018 Oct; (IF 4.679)

106. Li W,Ji M,Lin Y,Miao Y,Chen S,Li H
DEPP/DEPP1/C10ORF10 regulates hepatic glucose and fat metabolism partly via ROS-induced FGF21.
FASEB J. 2018 Oct; (IF 4.966)

107. Yang Y,Gao J,Zhang Y,Xu W,Hao Y,Xu Z,Tao L
Natural pyrethrins induce autophagy of HepG2 cells through the activation of AMPK/mTOR pathway.
Environ Pollut. 2018 Oct; (IF 6.792)

108. Zhou L,Yu M,Arshad M,Wang W,Lu Y,Gong J,Gu Y,Li P,Xu L
Coordination Among Lipid Droplets, Peroxisomes, and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in Adipocytes.
Diabetes. 2018 Oct; (IF 7.72)

109. Lv Y,Cheng X,Wu D,Du G,Zhou J,Chen J
Improving bioconversion of eugenol to coniferyl alcohol by in situ eliminating harmful H2O2.
BIORESOURCE TECHNOL. 2018 Nov; (IF 7.539)

110. Zhang D,Li Y,Zhang T,Liu J,Jahejo AR,Yang L,Chen P,Ning G,Huo N,Ma H,Yan F,Tian W
Protective effects of zinc and N-acetyl-L-cysteine supplementation against cadmium induced erythrocyte cytotoxicity in Arbor Acres broiler chickens (Gallus gallus domesticus).
ECOTOX ENVIRON SAFE. 2018 Nov 15; (IF 4.872)

111. Wang XX,Zhang TY,Dao GH,Hu HY
Interaction between 1,2-benzisothiazol-3(2H)-one and microalgae: Growth inhibition and detoxification mechanism.
Aquat Toxicol . 2018 Dec;205:66-75. (IF 4.344)

112. Xu S,Zhao X,Liu F,Cao Y,Wang B,Wang X,Yin M,Wang Q,Feng X
Crucial role of oxidative stress in bactericidal effect of parthenolide against Xanthomonas oryzae pv. oryzae.
Pest Manag Sci . 2018 Dec;74(12):2716-2723. (IF 3.75)

113. Wu Z,Wang Y,Meng X,Wang X,Li Z,Qian S,Wei Y,Shu L,Ding Y,Wang P,Peng Y
Total C-21 steroidal glycosides, isolated from the root tuber of Cynanchum auriculatum Royle ex Wight, attenuate hydrogen peroxide-induced oxidative injury and inflammation in L02 cells.
Int J Mol Med . 2018 Dec;42(6):3157-3170. (IF 3.098)

114. Liang Z,Yuan Z,Li G,Fu F,Shan Y
Hypolipidemic, Antioxidant, and Antiapoptotic Effects of Polysaccharides Extracted from Reishi Mushroom, Ganoderma lucidum (Leysser: Fr) Karst, in Mice Fed a High-Fat Diet.
J Med Food . 2018 Dec;21(12):1218-1227. (IF 1.981)

115. Wang Z,Zhou Y,Yu Y,He K,Cheng LM
Lipopolysaccharide preconditioning increased the level of regulatory B cells in the spleen after acute ischaemia/reperfusion in mice.
Brain Res . 2018 Dec 15;1701:46-57. (IF 2.733)

116. Li YJ,Li P,Wang T,Zhang FJ,Huang XX,Hou BK
The maize secondary metabolism glycosyltransferase UFGT2 modifies flavonols and contributes to plant acclimation to abiotic stresses.
ANN BOT-LONDON . 2018 Dec 31;122(7):1203-1217. (IF 4.005)

117. Ren C,Wu H,Li D,Yang Y,Gao Y,Jizhang Y,Liu D,Ji X,Zhang X
Remote Ischemic Conditioning Protects Diabetic Retinopathy in Streptozotocin-induced Diabetic Rats via Anti-Inflammation and Antioxidation.
Aging Dis . 2018 Dec 4;9(6):1122-1133. (IF 5.402)

118. Xu N,Wang Q,Jiang S,Wang Q,Hu W,Zhou S,Zhao L,Xie L,Chen J,Wellstein A,Lai EY
Fenofibrate improves vascular endothelial function and contractility in diabetic mice.
Redox Biol. 2019 Jan;20:87-97. (IF 9.986)

119. Zhuang S,Zhong J,Bian Y,Fan Y,Chen Q,Liu P,Liu Z
Rhein ameliorates lipopolysaccharide-induced intestinal barrier injury via modulation of Nrf2 and MAPKs.
Life Sci. 2019 Jan 1;216:168-175. (IF 3.647)

120. Jiang L,Guo YJ,Zhang F,Zhang YY,McCook LJ,Yuan XC,Lei XM,Zhou GW,Guo ML,Cai L,Lian JS,Qian PY,Huang H
Diurnally Fluctuating pCO2 Modifies the Physiological Responses of Coral Recruits Under Ocean Acidification.
Front Physiol. 2019 Jan 11; (IF 3.367)

121. Chen X,Yin H,Li G,Wang W,Wong PK,Zhao H,An T
Antibiotic-resistance gene transfer in antibiotic-resistance bacteria under different light irradiation: Implications from oxidative stress and gene expression.
Water Res. 2019 Feb 1;149:282-291. (IF 9.13)

122. Pan X,Song X,Wang C,Cheng T,Luan D,Xu K,Tang B
H2Se Induces Reductive Stress in HepG2 Cells and Activates Cell Autophagy by Regulating the Redox of HMGB1 Protein under Hypoxia.
Theranostics. 2019 Feb 28;9(6):1794-1808. (IF 8.579)

123. Zhang C,Yang Y,Liang W,Wang T,Wang S,Wang X,Wang Y,Jiang H,Feng H
Neuroprotection by urate on the mutant hSOD1-related cellular and Drosophila models of amyotrophic lateral sclerosis: Implication for GSH synthesis via activating Akt/GSK3β/Nrf2/GCLC pathways.
Brain Res Bull. 2019 Mar;146:287-301. (IF 3.37)

124. Zhang H,Chen Y,Pei Z,Gao H,Shi W,Sun M,Xu Q,Zhao J,Meng W,Xiao K
Protective effects of polydatin against sulfur mustard-induced hepatic injury.
TOXICOL APPL PHARM . 2019 Mar 15;367:1-11. (IF 3.616)

125. Zhang J,Yue X,Luo H,Jiang W,Mei Y,Ai L,Gao G,Wu Y,Yang H,An J,Ding S,Yang X,Sun B,Luo W,He R,Jia J,Lyu J,Tong Z
Illumination with 630 nm Red Light Reduces Oxidative Stress and Restores Memory by Photo-Activating Catalase and Formaldehyde Dehydrogenase in SAMP8 Mice.
ANTIOXID REDOX SIGN. 2019 Apr 10; (IF 6.323)

126. Quan XJ,Liang CL,Sun MZ,Zhang L,Li XL
Overexpression of steroid receptor coactivators alleviates hyperglycemia-induced endothelial cell injury in rats through activating the PI3K/Akt pathway.
Acta Pharmacol Sin. 2019 May; (IF 5.064)

127. Yue X,Zhang G,Zhao Z,Yue J,Pu X,Sui M,Zhan Y,Shi Y,Wang Z,Meng G,Zhao Z,An L
A Cryophyte Transcription Factor, CbABF1, Confers Freezing, and Drought Tolerance in Tobacco.
Front Plant Sci. 2019 May 31;10:699. (IF 4.402)

128. Zhu Z,Li R,Lv Y,Zeng W
Melatonin protects rabbit spermatozoa from cryo-damage via decreasing oxidative stress.
Cryobiology. 2019 Jun;88:1-8. (IF 2.283)

129. Xu X,Huang L,Zhang Z,Tong J,Mi J,Wu Y,Zhang C,Yan H
Targeting non-oncogene ROS pathway by alantolactone in B cell acute lymphoblastic leukemia cells.
Life Sci. 2019 Jun 15;227:153-165. (IF 3.647)

130. Tang Y,Bao X,Wang S,Liu Y,Tan J,Yang M,Zhang M,Dai R,Yu X
A Physic Nut Stress-Responsive HD-Zip Transcription Factor, JcHDZ07, Confers Enhanced Sensitivity to Salinity Stress in Transgenic Arabidopsis.
Front Plant Sci. 2019 Jul 17;10:942. (IF 4.402)

131. Wu J,Pan W,Wang C,Dong H,Xing L,Hou J,Fang S,Li H,Yang F,Yu B
H2S attenuates endoplasmic reticulum stress in hypoxia-induced pulmonary artery hypertension.
BIOSCIENCE REP. 2019 Jul 8;39(7). pii: BSR20190304. (IF 2.942)

132. Li Y,Zhang J,Liu H,Yuan J,Yin Y,Wang T,Cheng B,Sun S,Guo Z
Curcumin ameliorates glyoxylate-induced calcium oxalate deposition and renal injuries in mice.
Phytomedicine. 2019 Aug;61:152861. (IF 4.268)

133. Zhou Y,Song L,Ni S,Zhang Y,Zhang S
Administration of rGDF11 retards the aging process in male mice via action of anti-oxidant system.
Biogerontology. 2019 Aug;20(4):433-443. (IF 3.667)

134. Xiao X,Cheng Y,Song D,Li X,Hu Y,Lu Z,Wang F,Wang Y
Selenium-enriched Bacillus paralicheniformis SR14 attenuates H2O2-induced oxidative damage in porcine jejunum epithelial cells via the MAPK pathway.
APPL MICROBIOL BIOT. 2019 Aug;103(15):6231-6243. (IF 3.53)

135. Yuan Y,Chen Y,Peng T,Li L,Zhu W,Liu F,Liu S,An X,Luo R,Cheng J,Liu J,Lu Y
Mitochondrial ROS-induced lysosomal dysfunction impairs autophagic flux and contributes to M1 macrophage polarization in a diabetic condition.
CLIN SCI. 2019 Aug 14;133(15):1759-1777. (IF 5.223)

136. Jiang C,Qi Z,He W,Li Z,Tang Y,Wang Y,Huang Y,Zang H,Yang H,Liu J
Dynamically enhancing plaque targeting via a positive feedback loop using multifunctional biomimetic nanoparticles for plaque regression.
J Control Release. 2019 Aug 28;308:71-85. (IF 7.727)

137. Lin H,Zhang J,Ni T,Lin N,Meng L,Gao F,Luo H,Liu X,Chi J,Guo H
Yellow Wine Polyphenolic Compounds prevents Doxorubicin-induced cardiotoxicity through activation of the Nrf2 signalling pathway.
J Cell Mol Med. 2019 Sep;23(9):6034-6047. (IF 4.486)

138. Zhu Z,Li R,Fan X,Lv Y,Zheng Y,Hoque SAM,Wu,Zeng W
Resveratrol Improves Boar Sperm Quality via 5’AMP-Activated Protein Kinase Activation during Cryopreservation.
Oxid Med Cell Longev. 2019 Sep 4;2019:5921503. (IF 5.076)

139. Mo JL,Pan ZG,Chen X,Lei Y,Lv LL,Qian C,Sun FY
MicroRNA-365 Knockdown Prevents Ischemic Neuronal Injury by Activating Oxidation Resistance 1-Mediated Antioxidant Signals.
Neurosci Bull. 2019 Oct;35(5):815-825. (IF 4.326)

140. Fang J,Zhao X,Li S,Xing X,Wang H,Lazarovici P,Zheng W
Protective mechanism of artemisinin on rat bone marrow-derived mesenchymal stem cells against apoptosis induced by hydrogen peroxide via activation of c-Raf-Erk1/2-p90rsk-CREB pathway.
Stem Cell Res Ther. 2019 Oct 26;10(1):312. (IF 5.116)

141. Zhang C,Liang W,Wang H,Yang Y,Wang T,Wang S,Wang X,Wang Y,Feng H
γ-Oryzanol mitigates oxidative stress and prevents mutant SOD1-Related neurotoxicity in Drosophila and cell models of amyotrophic lateral sclerosis.
Neuropharmacology. 2019 Dec 1;160:107777. (IF 4.431)

142. Sha Luo,Jie Yin,Yang Peng,Jing Xie,Hetao Wu,Donglan He,Xiaohua Li,Guojun Cheng
Glutathione is Involved in Detoxification of Peroxide and Root Nodule Symbiosis of Mesorhizobium huakuii
Curr Microbiol. 2020 Jan;77(1):1-10.;doi: 10.1007/s00284-019-01784-8.

143. Xin Wang,Yanan Zhang,Shuai Han,Hongshen Chen,Chen Chen,Lingling Ji,Bihu Gao
Overexpression of miR‑30c‑5p reduces cellular cytotoxicity and inhibits the formation of kidney stones through ATG5
Int J Mol Med. 2020 Feb;45(2):375-384.;doi: 10.3892/ijmm.2019.4440.

144. Jiang H,Li R,Zhang Z,Chang C,Liu Y,Liu Z,He Q,Wang Q
Retinoid X receptor α (RXRα)-mediated erythroid-2-related factor-2 (NRF2) inactivation contributes to N,N-dimethylformamide (DMF)-induced oxidative stress in HL-7702 and HuH6 cells.
J Appl Toxicol. 2020 Apr;40(4):470-482. (IF 2.997)

145. Wei Y,Liu W,Hu W,Yan Y,Shi H
The chaperone MeHSP90 recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava.
New Phytol. 2020 Apr;226(2):476-491. (IF 8.512)

146. Hongmei Jiang,Ruobi Li,Zhen Zhang,Chong Chang,Ye Liu,Ziqi Liu,Qianmei He,Qing Wang
Retinoid X receptor α (RXRα)-mediated erythroid-2-related factor-2 (NRF2) inactivation contributes to N,N-dimethylformamide (DMF)-induced oxidative stress in HL-7702 and HuH6 cells
J Appl Toxicol. 2020 Apr;40(4):470-482.;doi: 10.1002/jat.3919.

147. Rohini Samadarsi,Debjani Dutta
Anti-oxidative effect of mangiferin-chitosan nanoparticles on oxidative stress-induced renal cells
Int J Biol Macromol. 2020 May 15;151:36-46.;doi: 10.1016/j.ijbiomac.2020.02.112.

148. Wenjin Guo,Juxiong Liu,Jingxuan Sun,Qian Gong,He Ma,Xingchi Kan,Yu Cao,Jianfa Wang,Shoupeng Fu
Butyrate alleviates oxidative stress by regulating NRF2 nuclear accumulation and H3K9/14 acetylation via GPR109A in bovine mammary epithelial cells and mammary glands
Free Radic Biol Med. 2020 May 20;152:728-742.;doi: 10.1016/j.freeradbiomed.2020.01.016.

149. Song Zhou,Yunjing Sun,Kai Zhao,Yanzhou Gao,Jiangman Cui,Liping Qi,Lingfang Huang
miR‑21/PTEN pathway mediates the cardioprotection of geniposide against oxidized low‑density lipoprotein‑induced endothelial injury via suppressing oxidative stress and inflammatory response
Int J Mol Med. 2020 May;45(5):1305-1316.;doi: 10.3892/ijmm.2020.4520.

150. Tao Wang,Ling Xu,Lei Gao,Lin Zhao,Xin-Hong Liu,Yan-Yan Chang,Yun-Lin Liu
Paeoniflorin attenuates early brain injury through reducing oxidative stress and neuronal apoptosis after subarachnoid hemorrhage in rats
Metab Brain Dis. 2020 Aug;35(6):959-970.;doi: 10.1007/s11011-020-00571-w.

151. Xue-Li Qiao,Qing-Jian Liang,Yuan Liu,Wei-Na Wang
A Novel Kelch-Like-1 Is Involved in Antioxidant Response by Regulating Antioxidant Enzyme System in Penaeus vannamei
Genes (Basel). 2020 Sep 15;11(9):1077.;doi: 10.3390/genes11091077.

152. Zhicheng Zhang,Li Wang,Yawen Ding,Jinhui Wu,Yiqiao Hu,Ahu Yuan
Synergy of hypoxia relief and chromatin remodeling to overcome tumor radiation resistance
Biomater Sci. 2020 Sep 7;8(17):4739-4749.;doi: 10.1039/d0bm00119h.

153. Yi-Qing Wang,Ji-Xiang Huang,Wen-Wen Zhou
Isolation, characterization and cytoprotective effects against UV radiation of exopolysaccharide produced from Paenibacillus polymyxa PYQ1
J Biosci Bioeng. 2020 Sep;130(3):283-289.;doi: 10.1016/j.jbiosc.2020.05.001.

154. Cai Liu,Dongqiang He,Qiming Zhao
Licoricidin improves neurological dysfunction after traumatic brain injury in mice via regulating FoxO3/Wnt/β-catenin pathway
J Nat Med. 2020 Sep;74(4):767-776.;doi: 10.1007/s11418-020-01434-5.

155. Yu Zhang,Tong-Tong Ji,Ting-Ting Li,Yang-Yang Tian,Lin-Feng Wang,Wen-Cheng Liu
Jasmonic acid promotes leaf senescence through MYC2-mediated repression of CATALASE2 expression in Arabidopsis
Plant Sci. 2020 Oct;299:110604.;doi: 10.1016/j.plantsci.2020.110604.

156. Yangyang Chen,Jichun Tang,Yinghong Zhang,Juan Du,Yuanli Wang,Hui Yu,Yanling He
Astaxanthin alleviates gestational diabetes mellitus in mice through suppression of oxidative stress
Naunyn Schmiedebergs Arch Pharmacol. 2020 Dec;393(12):2517-2527.;doi: 10.1007/s00210-020-01861-x.

总谷胱甘肽检测试剂盒(S0052)

总谷胱甘肽检测试剂盒

产品编号: S0052

产品包装:100次
选择包装
100次

说明书下载

389.00 10
价格: ¥ 389.00

产品简介
使用说明
产品图片
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
S0052 总谷胱甘肽检测试剂盒 100次 389.00元

总谷胱甘肽检测试剂盒(Total Glutathione Assay Kit)是一种简单易行的检测总谷胱甘肽(GSSG+GSH)的试剂盒。
谷胱甘肽(glutathione)是一种由3个氨基酸残基组成的小肽,全称为谷氨酰-半胱氨酰-甘氨酸,英文名称为glutamyl-cysteinyl-glycine,简称为glutathione。由于半胱氨酸上的巯基(SH)为谷胱甘肽的活性基团,所以常简写为G-SH或GSH。谷胱甘肽包括还原型谷胱甘肽(reduced glutathione, 常称为GSH)和氧化型谷胱甘肽(oxidized glutathione disulfide)两种形式。由于氧化型谷胱甘肽是由两个GSH通过巯基脱氢而成,所以常简写为G-S-S-G或GSSG。还原型谷胱甘肽是绝大多数活细胞中巯基的主要来源,对于维持蛋白质中巯基适当的氧化还原状态有重要作用,并且是动物细胞中关键的抗氧化剂。总谷胱甘肽中通常90-95%为还原型谷胱甘肽。
通过谷胱甘肽还原酶把氧化型谷胱甘肽(GSSG)还原成还原型谷胱甘肽(GSH),而GSH可以和生色底物DTNB反应产生黄色的TNB和GSSG。适当配制反应体系,前后两个反应合并起来后,总谷胱甘肽(GSSG+GSH)就相当于一个颜色产生的限速因素,总谷胱甘肽的量就决定了黄色的TNB形成量。从而通过测定A412就可以计算出总谷胱甘肽的量。
本试剂盒的具体反应原理如下:

总谷胱甘肽检测试剂盒(S0052)
总谷胱甘肽检测试剂盒(S0052)

两个反应相合并:

总谷胱甘肽检测试剂盒(S0052)

本试剂盒可以检测动物组织、血浆、红细胞、和培养细胞或其它适当样品中总谷胱甘肽的含量。
本试剂盒提供了蛋白去除试剂S,可以更加准确地测定出含有蛋白的样品中的总谷胱甘肽的量。
本试剂盒的检测下限为1μM。一个试剂盒共可以进行100次检测。
包装清单:

产品编号 产品名称 包装
S0052-1 总谷胱甘肽检测缓冲液 60ml
S0052-2 谷胱甘肽还原酶 150μl
S0052-3 还原型谷胱甘肽(GSH) 4.5mg
S0052-4 DTNB 4.5mg
S0052-5 蛋白去除试剂S 0.4g
S0052-6 NADPH 4mg
S0052-7 DMSO 1.5ml
说明书 1份

保存条件:
-20℃保存,一年有效。还原型谷胱甘肽配制成溶液后,需适当分装,-20℃保存至少3个月有效。DTNB溶解在DMSO中后,需适当分装,-20℃保存至少3个月有效。蛋白去除试剂S配制成溶液后可以4℃保存。NADPH溶解后,适当分装,-70℃保存。
注意事项:
本试剂盒检测时牵涉到氧化还原反应,所有氧化剂或还原剂都会干扰本试剂盒的测定。特别是DTT、巯基乙醇等含有巯基的试剂会严重干扰本试剂盒的测定,请尽量避免。
一定要严格控制反应时的温度和反应时间,否则需每次都做标准曲线。
NADPH等试剂不太稳定,要严格按照后续说明操作,谨防失活。
DMSO在4℃、冰浴等较低温度情况下会凝固,可以20-25℃水浴温育片刻至全部融解后使用。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 试剂盒的准备工作:
a.GSH储备液的配制:在本试剂盒提供的4.5mgGSH中加入1.5毫升Milli-Q级纯水,溶解并混匀,即为GSH储备液,浓度为10mM。除立即待用部分外,其余GSH储备液适当分装后-20℃保存。
b.DTNB储备液的配制:在本试剂盒提供的4.5mg DTNB中加入1.5毫升本试剂盒提供的DMSO,溶解并混匀,即为DTNB储备液。除立即待用部分外,其余DTNB储备液适当分装后-20℃保存。
c.蛋白去除试剂S溶液的配制:在本试剂盒提供的0.4克蛋白去除试剂S中加入8毫升Milli-Q级纯水,配制成8毫升5%的水溶液。4℃保存。
d.NADPH储备液(40mg/ml)的配制:在本试剂盒提供的4mg NADPH中加入100微升Milli-Q级纯水,溶解并混匀,即为NADPH储备液。除立即待用部分外,其余NADPH储备液适当分装后-70℃保存。
e.5倍稀释谷胱甘肽还原酶的配制:取50微升谷胱甘肽还原酶,加入200微升总谷胱甘肽检测缓冲液,混匀,即成5倍稀释的谷胱甘肽还原酶。
f.总谷胱甘肽检测工作液的配制:根据待检测的样品数参考下表配制适当量的总谷胱甘肽检测工作液,表中三种试剂按比例混合后即为总谷胱甘肽检测工作液。

1个样品 10个样品 20个样品
5倍稀释谷胱甘肽还原酶 6.6 μl 66 μl 132 μl
DTNB储备液 6.6 μl 66 μl 132 μl
总谷胱甘肽检测缓冲液 150 μl 1.5 ml 3 ml

g.0.5mg/ml NADPH的配制:取10微升NADPH储备液,加入790微升总谷胱甘肽检测缓冲液,混匀即为0.5mg/ml NADPH。每检测一个样品需50微升0.5mg/ml NADPH。
2. 标准品的准备:
把10mM GSH储备液用蛋白去除试剂S溶液稀释成50μM GSH溶液。然后依次稀释成25、15、10、5、2μM GSH溶液。取50、25、15、10、5、2μM GSH溶液六个点做标准曲线。注意:由于GSH在蛋白去除试剂S溶液中不太稳定,用蛋白去除试剂S溶液配制的GSH溶液必须新鲜配制后使用,不可冻存后再使用。
3. 样品的准备:
a.组织样品的准备。取组织用液氮速冻,然后研成粉末。每10毫克研碎的组织粉末,加入30微升蛋白去除试剂S溶液,充分Vortex。再加入70微升蛋白去除试剂S溶液,用玻璃匀浆器充分匀浆(对于比较容易匀浆的组织可以不用液氮速冻等处理,而直接加入适量蛋白去除试剂S溶液进行匀浆)。4℃放置10分钟后,10,000g 4℃离心10分钟,取上清用于总谷胱甘肽的测定。样品需暂时4℃保存,不立即测定的样品可以-70℃保存,但不宜超过10天。对于处理好的组织样品通常需用蛋白去除试剂S溶液进行适当稀释后再进行测定,稀释倍数通常为5-20倍。
b.细胞样品的准备。请尽量使用新鲜的细胞进行测定,而不要使用冻存的细胞进行测定。PBS洗涤细胞一次,离心收集细胞,吸尽上清。加入细胞沉淀体积3倍量的蛋白去除试剂S溶液,即如果细胞沉淀为10微升,则加入30微升蛋白去除试剂S溶液,充分Vortex。(细胞沉淀的体积可以根据细胞沉淀的重量进行估算。收集细胞前后分别对离心管进行称重,从而就可以计算出细胞沉淀的重量。10毫克细胞沉淀的体积可以粗略地看做10微升。) 然后利用液氮和37℃水浴对样品进行两次快速的冻融。4℃或冰浴放置5分钟。4℃,10,000g离心10分钟。取上清用于总谷胱甘肽的测定。样品需暂时4℃保存,不立即测定的样品可以-70℃保存,但不宜超过10天。对于处理好的细胞样品通常需用蛋白去除试剂S溶液进行适当稀释后再进行测定,稀释倍数可以高达20倍。
c.红细胞或血浆样品的准备。请尽量使用新鲜的血液进行测定。600g离心10分钟,沉淀为红细胞,上清为血浆。对于红细胞,用PBS洗涤两次。取约50微升红细胞沉淀或血浆,加入50微升蛋白去除试剂S溶液,充分Vortex。4℃或冰浴放置10分钟。4℃,10,000g离心10分钟。取上清用于总谷胱甘肽的测定。样品需暂时4℃保存,不立即测定的样品可以-70℃保存,但不宜超过10天。对于处理好的红细胞样品最后需用蛋白去除试剂S溶液稀释10倍后再进行后续的测定,而对于血浆样品,应直接取10微升进行测定。
d.对于一些谷胱甘肽含量特别低的样品,可以通过冷冻干燥进行浓缩后再进行测定。
4.样品和标准品的测定:
a.参考下表,使用96孔板,依次加入样品或标准品,混匀。加入150微升总谷胱甘肽检测工作液后,混匀,25℃或室温孵育5分钟。

空白对照 (blank) 标准曲线 (standard) 样品(sample)
样品或标准品 0 μl 10 μl x μl
蛋白去除试剂S溶液 10 μl 0 μl 10-x μl
总谷胱甘肽检测工作液 150 μl 150 μl 150 μl
25℃或室温孵育 5 min 5 min 5 min
0.5mg/ml NADPH 50 μl 50 μl 50 μl

b.加入50微升0.5mg/ml NADPH溶液,混匀。
c.立即用酶标仪测定A412,每5分钟测定一次或实时测定,共测定25分钟,测得5个数据。(说明:为了简化实验步骤,可以在加入NADPH溶液混匀后25分钟,仅测定一次A412)。如果仪器可以设置温度,把温度设置在25℃,否则就在室温状况下测定。如果酶标仪不能测定A412,可以测定405-414nm附近范围的吸光度。如果标准曲线良好,但样品的吸光度比较低,可以延长孵育时间至30-60分钟,标准品和样品的吸光度在一定范围内会随时间的延长接近于线性增加的。
d.标准品的实测效果参考图1。
总谷胱甘肽检测试剂盒(S0052) 图1. GSH标准曲线实测效果图。图中数据仅供参考,实际的检测效果可能会因具体反应条件的不同而有所不同。 5.样品中总谷胱甘肽含量的计算:
a.单点测定法:即反应25分钟(或30-60分钟)后仅测定一次吸光度。根据不同浓度标准品测得的不同吸光度作出标准曲线。样品对照标准曲线即可计算出总谷胱甘肽的含量。实际计算出来的总谷胱甘肽的含量相当于把氧化型谷胱甘肽的含量乘以2再加上还原型谷胱甘肽的含量。单点法测定相对比较便捷,而动力学法测定则相对比较精确。
b.动力学测定法:先根据不同时间点测定得到的吸光度值计算出ΔA412/min。然后以标准品的浓度为横坐标,以ΔA412/min为纵坐标,做出标准曲线。根据样品的ΔA412/min,对照标准曲线就可以计算出测定时样品中总谷胱甘肽的含量。
c.同时根据样品的稀释倍数、以及最初样品的使用量,可以计算出每毫克组织或细胞中的总谷胱甘肽的含量。对于细胞样品,也可以根据最初细胞的使用数量,然后另外取一定数量的细胞裂解后测定蛋白浓度,从而计算出细胞样品的蛋白量,最后计算出每毫克蛋白中总谷胱甘肽的含量。

相关产品:

产品编号 产品名称 包装
S0052 总谷胱甘肽检测试剂盒 100次
S0052B 蛋白去除试剂S 3g
S0053 GSH和GSSG检测试剂盒 共100次
S0054 蛋白去除试剂M 5g
S0055 谷胱甘肽还原酶检测试剂盒 100次
S0056 谷胱甘肽过氧化物酶检测试剂盒 100次
S0058 总谷胱甘肽过氧化物酶检测试剂盒 100次

总谷胱甘肽检测试剂盒(S0052)

相关产品请点击如下按钮:
活性氧相关

使用本产品的文献:
1. Xiao-Long W, Chuan-Ping Y, Kai X, Ou-Jv Q.
Selenoprotein W depletion in vitro might indicate that its main function is not as an antioxidative enzyme.
BIOCHEMISTRY-MOSCOW+ . 2010;75(2):201-7. (IF 1.978)

2. Wu X, Zhu Y, Yan H, Liu B, Li Y, Zhou Q, Xu K.
Isothiocyanates induce oxidative stress and suppress the metastasis potential of human non-smallcell lung cancer cells.
BMC Cancer . 2010 Jun 9;10:269. (IF 3.15)

3. Chen L, Sun BB, Wang T, Wang X, Li JQ, Wang HX, Zhang SF, Liu DS, Liu L, Xu D, Ou XM, Chen YJ, Yang T, Zhou H, Wen FQ.
Cigarette smoke enhances {beta}-defensin 2 expression in rat airways via nuclear factor-{kappa}B activation.
Eur Respir J . 2010 Sep;36(3):638-45. (IF 12.339)

4. Zhang X, Zhao X, Ma Z.
PYDDT, a novel phase 2 enzymes inducer, activates Keap1-Nrf2 pathway via depleting the cellularlevel of glutathione.
Toxicol Lett . 2010 Nov 10;199(1):93-101. (IF 3.569)

5. Wang J, Sun P, Bao Y, Liu J, An L.
Cytotoxicity of single-walled carbon nanotubes on PC12 cells.
Toxicol In Vitro . 2011 Feb;25(1):242-50. (IF 2.959)

6. Bao HG, Wang XY, Li JY, Wu CX.
Comparison of effects of hypoxia on glutathione and activities of related enzymes in livers of Tibetchicken and Silky chicken.
J POULT SCI . 2011 Mar;90(3):648-52. (IF 0.88)

7. Zhang J, Zhao QZ, Duan GL, Huang YC.
Influence of sulphur on arsenic accumulation and metabolism in rice seedlings.
Environmental and Experimental Botany . 2011 Aug;72(1):34-40. (IF 3.712)

8. Li DY, Xue MY, Geng ZR, Chen PY.
The suppressive effects of Bursopentine (BP5) on oxidative stress and NF-?B activation in lipopolysaccharide-activated murine peritoneal macrophages.
CELL PHYSIOL BIOCHEM . 2012;29(1-2):9-20. (IF 5.5)

9. Zhao G, Yao-Yue C, Qin GW, Guo LH.
Luteolin from Purple Perilla mitigates ROS insult particularly in primary neurons.
Neurobiol Aging . 2012 Jan;33(1):176-86. (IF 4.347)

10. Liu JH, Qi ZM, Huang Q, Wei XL, Ke ZG, Fang YS, Tian YC, Yu ZL.
Study of energetic-particle-irradiation induced biological effect on Rhizopus oryzae through Synchrotron-FTIR micro-spectroscopy.
Journal of Molecular Structure . 2012Jan;1031(16):1-8. (IF 2.12)

11. Wang J, Sun P, Bao Y, Dou B, Song D, Li Y.
Vitamin E renders protection to PC12 cells against oxidative damage and apoptosis induced bysingle-walled carbon nanotubes.
Toxicol In Vitro . 2012 Feb;26(1):32-41. (IF 2.959)

12. Xu HL, Yu XF, Qu SC, Qu XR, Jiang YF, Sui da Y.
Juglone, from Juglans mandshruica Maxim, inhibits growth and induces apoptosis in humanleukemia cell HL-60 through a reactive oxygen species-dependent mechanism.
Food Chem Toxicol . 2012 Mar;50(3-4):590-6. (IF 4.679)

13. Chen T, Zhang L, Yue JQ, Lv ZQ, Xia W, Wan YJ, Li YY, Xu SQ.
Prenatal PFOS exposure induces oxidative stress and apoptosis in the lung of rat off-spring.
Reprod Toxicol . 2012 Jul;33(4):538-45. (IF 3.121)

14. Lou H, Jing X, Ren D, Wei X, Zhang X.
Eriodictyol protects against H(2)O(2)-induced neuron-like PC12 cell death through activation of Nrf2/ARE signaling pathway.
Neurochem Int . 2012 Jul;61(2):251-7. (IF 3.881)

15. Ou XH, Li S, Wang ZB, Li M, Quan S, Xing F, Guo L, Chao SB, Chen Z, Liang XW, Hou Y, Schatten H, Sun QY.
Maternal insulin resistance causes oxidative stress and mitochondrial dysfunction in mouse oocytes.
Hum Reprod . 2012 Jul;27(7):2130-45. (IF 5.733)

16. Fan S, Yu Y, Qi M, Sun Z, Li L, Yao G, Tashiro S, Onodera S, Ikejima T.
P53-mediated GSH depletion enhanced the cytotoxicity of NO in silibinin-treated human cervicalcarcinoma HeLa cells.
FREE RADICAL RES . 2012 Sep;46(9):1082-92. (IF 2.839)

17. Wang Y, Wang L, Zhu Z, Ma W, Lei C.
The molecular characterization of antioxidant enzyme genes in Helicoverpa armigera adults and their involvement in response to ultraviolet-A stress.
J Insect Physiol . 2012 Sep;58(9):1250-8. (IF 2.246)

18. Lin M, Xia B, Yang M, Gao S, Huo Y, Lou G.
Characterization and antitumor activities of a polysaccharide from the rhizoma of Menispermum dauricum.
Int J Biol Macromol . 2013 Feb;53:72-6. (IF 5.162)

19. Yang L, Qu M, Wang Y, Duan H, Chen P, Wang Y, Shi W, Danielson P, Zhou Q.
Trichostatin A inhibits transforming growth factor-β-induced reactive oxygen species accumulation andmyofibroblast differentiation via enhanced NF-E2-related factor 2-antioxidant response element signaling.
Mol Pharmacol . 2013 Mar;83(3):671-80. (IF 3.664)

20. Liu BN, Yan HQ, Wu X, Pan ZH, Zhu Y, Meng ZW, Zhou QH, Xu K.
Apoptosis induced by benzyl isothiocyanate in gefitinib-resistant lung cancer cells is associated with Akt/MAPK pathways and generation of reactive oxygen species.
Cell Biochem Biophys . 2013 May;66(1):81-92. (IF 2.073)

21. Zhou Q, Duan H, Wang Y, Qu M, Yang L, Xie L.
ROCK inhibitor Y-27632 increases the cloning efficiency of limbal stem/progenitor cells by improving theiradherence and ROS-scavenging capacity.
TISSUE ENG PART C-ME . 2013 Jul;19(7):531-7. (IF 2.608)

22. Liu X, Chen L, Feng B, Liu G.
Reversing effect of sorcin in the drug resistance of human nasopharyngeal carcinoma.
ANAT REC . 2014 Feb;297(2):215-21. (IF 1.634)

23. Ji X, Wang H, Zhu J, Zhu L, Pan H, Li W, Zhou Y, Cong Z, Yan F, Chen S.
Knockdown of Nrf2 suppresses glioblastoma angiogenesis by inhibiting hypoxia-induced activation of HIF-1α.
Int J Cancer . 2014 Aug 1;135(3):574-84. (IF 5.145)

24. Gao Y, Li W, Liu X, Gao F, Zhao X.
Reversing effect and mechanism of soluble resistance-related calcium-binding protein on multidrug resistance in human lung cancer A549/DDP cells.
Mol Med Rep . 2015 Mar;11(3):2118-24. (IF 2.1)

25. Han S, Lv X, Wang Y, Gong H, Zhang C, Tong A, Zhang B, Yao H.
Effect and mechanism of peroxisome proliferator-activated receptor-γ on the drug resistance of the U-87 MG/CDDP human malignant glioma cell line.
Mol Med Rep . 2015 Aug;12(2):2239-46. (IF 2.1)

26. Xie X, Zhao Y, Ma CY, Xu XM, Zhang YQ, Wang CG, Jin J, Shen X, Gao JL, Li N, Sun ZJ, Dong DL.
Dimethyl fumarate induces necroptosis in colon cancer cells through GSH depletion/ROS increase/MAPKs activation pathway.
BRIT J PHARMACOL . 2015 Aug;172(15):3929-43. (IF 7.73)

27. Yuan ZY, Hu YL, Gao JQ.
Brain Localization and Neurotoxicity Evaluation of Polysorbate 80-Modified Chitosan Nanoparticles in Rats.
PLoS One . 2015 Aug 6;10(8):e0134722. (IF 2.74)

28. Wang C, Wang G, Liu H, Hou YL.
Protective effect of bioactive compounds from Lonicera japonica Thunb. against H2O2-induced cytotoxicityusing neonatal rat cardiomyocytes.
Iran J Basic Med Sci . 2016 Jan;19(1):97-105. (IF 2.146)

29. Liu X, Li H, Liu L, Lu Y, Gao Y, Geng P, Li X, Huang B, Zhang Y, Lu J.
Methylation of arginine by PRMT1 regulates Nrf2 transcriptional activity during the antioxidative response.
BBA-BIOMEMBRANES . 2016 Aug;1863(8):2093-103. (IF 3.411)

30. Zhang T, Zhang C, Fan X, Li R, Zhang J.
Effect of C-type natriuretic peptide pretreatment on in vitro bovine oocyte maturation.
IN VITRO CELL DEV-AN . 2016 Oct 19.[Epub ahead of print] (IF 1.665)

31. Jia Y, Wang HD, Wang Q, Ding H, Wu HM, Pan H.
GSH depletion and consequent AKT inhibition contribute tothe Nrf2 knockdown-induceddecrease in proliferation in glioblastoma U251 cells.
Oncol Rep . 2017 Apr;37(4):2252-2260. (IF 3.417)

32. Han X, Xue X, Zhao Y, Li Y, Liu W, Zhang J, Fan S.
Rutin-Enriched Extract from Coriandrum sativum L. Ameliorates Ionizing Radiation-InducedHematopoietic Injury.
Int J Mol Sci . 2017 Apr 29;18(5). pii: E942. (IF 4.556)

33. Cen J, Zheng B, Bai R, Zhang L, Zhang F, Zhang X.
Triterpenoids from Aglaia abbreviata exert cytotoxicity and multidrug resistant reversal effect in MCF-7/ADM cells via reactive oxygen species induction and P-glycoprotein inhibition.
ONCOTARGET . 2017 Apr 20;8(41):69465-69476. (IF 5.168)

34. Zhang R, Shao M, Han X, Wang C, Li Y, Hu B, Pang D, Xie Z.
ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSequantum dots biosynthesized in Saccharomyces cerevisiae.
INT J NANOMED . 2017 May 19;12:3865-3879. (IF 5.115)

35. Cai Y, Zhao B, Liang Q, Zhang Y, Cai J, Li G.
The selective effect of glycyrrhizin and glycyrrhetinic acid on topoisomerase IIαand apoptosis in combination with etoposide on triple negative breast cancer MDA-MB-231 cells.
Eur J Pharmacol . 2017 Aug 15;809:87-97. (IF 3.263)

36. Fu Q,Li C,Yu L
Gambogic acid inhibits spinal cord injury and inflammation through suppressing the p38 and Akt signaling pathways.
Mol Med Rep . 2018 Jan;17(1):2026-2032. (IF 2.1)

37. Ahangarpour A,Alboghobeish S,Oroojan AA,Dehghani MA
Mice pancreatic islets protection from oxidative stress induced by single-walled carbon nanotubes through naringin.
Hum Exp Toxicol . 2018 Jan 1:960327118769704. (IF 2.067)

38. Chen Y,Wang B,Zhao H
Thymoquinone reduces spinal cord injury by inhibiting inflammatory response, oxidative stress and apoptosis via PPAR-γ and PI3K/Akt pathways.
Exp Ther Med . 2018 Jun;15(6):4987-4994. (IF 1.785)

39. Liu M,Xiao C,Sun M,Tan M,Hu L,Yu Q
Parthenolide Inhibits STAT3 Signaling by Covalently Targeting Janus Kinases.
Molecules . 2018 Jun 19;23(6). pii: E1478. (IF 3.267)

40. Pan L,Zhou L,Yin W,Bai J,Liu R
miR-125a induces apoptosis, metabolism disorder and migrationimpairment in pancreatic cancer cells by targeting Mfn2-related mitochondrial fission.
Int J Oncol . 2018 Jul;53(1):124-136. (IF 3.899)

41. Tang SG,Liu XY,Ye JM,Hu TT,Yang YY,Han T,Tan W
Isosteviol ameliorates diabetic cardiomyopathy in rats by inhibiting ERK and NF-κB signaling pathways.
J Endocrinol . 2018 Jul;238(1):47-60. (IF 4.041)

42. Chen L,Zhang J,Li C,Wang Z,Li J,Zhao D,Wang S,Zhang H,Huang Y,Guo X
Glycine Transporter-1 and glycine receptor mediate the antioxidant effect of glycine in diabetic rat islets and INS-1 cells.
FREE RADICAL BIO MED . 2018 Aug 1;123:53-61. (IF 6.17)

43. Wei W,Luo C,Yang J,Sun B,Zhao D,Liu Y,Wang Y,Yang W,Kan Q,Sun J,He Z
Precisely albumin-hitchhiking tumor cell-activated reduction/oxidation-responsive docetaxel prodrugs for the hyperselective treatment of breast cancer.
J Control Release . 2018 Sep 10;285:187-199. (IF 7.727)

44. Wu X,Gao H,Hou Y,Yu J,Sun W,Wang Y,Chen X,Feng Y,Xu QM,Chen X
Dihydronortanshinone, a natural product, alleviates LPS-induced inflammatory response through NF-κB, mitochondrial ROS, and MAPK pathways.
TOXICOL APPL PHARM  . 2018 Sep 15;355:1-8. (IF 3.616)

45. Liu H,Huang H,Li R,Bi W,Feng L,E L,Hu M,Wen W
Mitophagy protects SH-SY5Y neuroblastoma cells against the TNFα-induced inflammatory injury: Involvement of microRNA-145 and Bnip3.
Biomed Pharmacother. 2019 Jan;109:957-968. (IF 4.545)

46. Zhang C,Yang Y,Liang W,Wang T,Wang S,Wang X,Wang Y,Jiang H,Feng H
Neuroprotection by urate on the mutant hSOD1-related cellular and Drosophila models of amyotrophic lateral sclerosis: Implication for GSH synthesis via activating Akt/GSK3β/Nrf2/GCLC pathways.
Brain Res Bull. 2019 Mar;146:287-301. (IF 3.37)

47. Wang QL,Guo C,Qi J,Ma JH,Liu FY,Lin SQ,Zhang CY,Xie WD,Zhuang JJ,Li X
Protective effects of 3β-angeloyloxy-8β, 10β-dihydroxyeremophila-7(11)-en-12, 8α-lactone on paraquat-induced oxidative injury in SH-SY5Y cells.
J Asian Nat Prod Res . 2019 Apr;21(4):364-376. (IF 1.345)

48. Chang G,Chen Y,Zhang H,Zhou W
Trans sodium crocetinate alleviates ischemia/reperfusion-induced myocardial oxidative stress and apoptosis via the SIRT3/FOXO3a/SOD2 signaling pathway.
Int Immunopharmacol. 2019 Jun;71:361-371. (IF 3.943)

49. Sun X,Wang S,Gai J,Guan J,Li J,Li Y,Zhao J,Zhao C,Fu L,Li Q
SIRT5 Promotes Cisplatin Resistance in Ovarian Cancer by Suppressing DNA Damage in a ROS-Dependent Manner via Regulation of the Nrf2/HO-1 Pathway.
Front Oncol. 2019 Aug 13;9:754. (IF 4.848)

50. Yang H,Shi Z,Wang XX,Cheng R,Lu M,Zhu J,Deng W,Zeng Y,Zhao LY,Zhang SY
Phenanthrene, but not its isomer anthracene, effectively activates both human and mouse nuclear receptor constitutive androstane receptor (CAR) and induces hepatotoxicity in mice.
TOXICOL APPL PHARM . 2019 Sep 1;378:114618. (IF 3.616)

51. Du K,Liu Q,Liu M,Lv R,He N,Wang Z
Encapsulation of glucose oxidase in Fe(III)/tannic acid nanocomposites for effective tumor ablation via Fenton reaction.
Nanotechnology. 2019 Sep 16;31(1):015101. (IF 3.551)

52. Li X,Yao Q,Huang J,Jin Q,Xu B,Chen F,Tu C
Morin Hydrate Inhibits TREM-1/TLR4-Mediated Inflammatory Response in Macrophages and Protects Against Carbon Tetrachloride-Induced Acute Liver Injury in Mice.
Front Pharmacol. 2019 Sep 20;10:1089. (IF 4.225)

53. Wang TX,Liang JY,Zhang C,Xiong Y,Guan KL,Yuan HX
The oncometabolite 2-hydroxyglutarate produced by mutant IDH1 sensitizes cells to ferroptosis.
Cell Death Dis. 2019 Oct 7;10(10):755. (IF 6.304)

54. Wang Y,Ji X,Yan M,Chen X,Kang M,Teng L,Wu X,Chen J,Deng C
Protective effect and mechanism of polysaccharide from Dictyophora indusiata on dextran sodium sulfate-induced colitis in C57BL/6 mice.
Int J Biol Macromol. 2019 Nov 1;140:973-984. (IF 5.162)

55. Hou L,Sun F,Sun W,Zhang L,Wang Q
Lesion of the Locus Coeruleus Damages Learning and Memory Performance in Paraquat and Maneb-induced Mouse Parkinson’s Disease Model.
Neuroscience. 2019 Nov 1;419:129-140. (IF 3.056)

56. Liu Q,Du K,Liu M,Lv R,Sun B,Cao D,He N,Wang Z
Sulfosalicylic acid/Fe3+ based nanoscale coordination polymers for effective cancer therapy by the Fenton reaction: an inspiration for understanding the role of aspirin in the prevention of cancer.
BIOMATER SCI-UK. 2019 Nov 19;7(12):5482-5491. (IF 6.183)

57. Zhang C,Liang W,Wang H,Yang Y,Wang T,Wang S,Wang X,Wang Y,Feng H
γ-Oryzanol mitigates oxidative stress and prevents mutant SOD1-Related neurotoxicity in Drosophila and cell models of amyotrophic lateral sclerosis.
Neuropharmacology. 2019 Dec 1;160:107777. (IF 4.431)

58. Sha Luo,Jie Yin,Yang Peng,Jing Xie,Hetao Wu,Donglan He,Xiaohua Li,Guojun Cheng
Glutathione is Involved in Detoxification of Peroxide and Root Nodule Symbiosis of Mesorhizobium huakuii
Curr Microbiol. 2020 Jan;77(1):1-10.;doi: 10.1007/s00284-019-01784-8.

59. Lingyun Xiao,Shu Liang,Lanlan Ge,Haoqiang Wan,Weigang Wu,Jia Fei,Shipin Wu,Boping Zhou,Xiaobin Zeng
4,5-di-O-caffeoylquinic acid methyl ester isolated from Lonicera japonica Thunb. targets the Keap1/Nrf2 pathway to attenuate H 2 O 2-induced liver oxidative damage in HepG2 cells
Phytomedicine. 2020 Apr 23;70:153219.;doi: 10.1016/j.phymed.2020.153219.

60. Pei Zhang,Ling Chen,Qiqi Zhao,Xixun Du,Mingxia Bi,Yong Li,Qian Jiao,Hong Jiang
Ferroptosis was more initial in cell death caused by iron overload and its underlying mechanism in Parkinson’s disease
Free Radic Biol Med. 2020 May 20;152:227-234.;doi: 10.1016/j.freeradbiomed.2020.03.015.

61. Qingye Zeng,Baishuang Zeng,Jinwei Chai,Jiena Wu,Ruiyin Guo,Yahua Gao,Xiaoyan Han,Jie Yang,Michail Kotsyfakis,Xueqing Xu
Antioxidant properties and neuroprotective effects of Esc-1GN through the regulation of MAPK and AKT signaling
Life Sci. 2020 Aug 1;254:117753.;doi: 10.1016/j.lfs.2020.117753.

蛋白去除试剂S(S0052B)

蛋白去除试剂S

产品编号: S0052B

产品包装:3g
选择包装
3g

说明书下载

86.00 10
价格: ¥ 86.00

产品简介
使用说明
产品图片
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
S0052B 蛋白去除试剂S 3g 86.00元

蛋白去除试剂S是用于碧云天生产的总谷胱甘肽检测试剂盒(S0052)的专用试剂。总谷胱甘肽检测试剂盒中已经提供了适量的蛋白去除试剂S,但在某些特定情况下,有些用户希望使用较大量的蛋白去除试剂S,可以购买本试剂用于总谷胱甘肽检测试剂盒的相关检测。
包装清单:

产品编号 产品名称 包装
S0052B 蛋白去除试剂S 3g
说明书 1份

保存条件:
4℃保存,一年有效。蛋白去除试剂S配制成溶液后仅限当天使用。
注意事项:
蛋白去除试剂S溶液必须新鲜配制并限当日使用。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
请参考碧云天生产的总谷胱甘肽检测试剂盒(S0052)的说明书使用蛋白去除试剂S。

蛋白去除试剂S(S0052B)

相关产品请点击如下按钮:
活性氧相关

使用本产品的文献:

蛋白去除试剂M(S0054)

蛋白去除试剂M

产品编号: S0054

产品包装:5g
选择包装
5g

说明书下载

139.00 10
价格: ¥ 139.00

产品简介
使用说明
产品图片
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
S0054 蛋白去除试剂M 5g 139.00元

蛋白去除试剂M是用于碧云天生产的GSH和GSSG检测试剂盒(S0053)的专用试剂。GSH和GSSG检测试剂盒中已经提供了适量的蛋白去除试剂M,但在某些特定情况下,有些用户希望使用较大量的蛋白去除试剂M,可以购买本试剂用于GSH和GSSG检测试剂盒的相关检测。
包装清单:

产品编号 产品名称 包装
S0054 蛋白去除试剂M 5g
说明书 1份

保存条件:
4℃保存,一年有效。蛋白去除试剂M配制成溶液后仅限当天使用。
注意事项:
蛋白去除试剂M溶液必须新鲜配制并限当日使用。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
请参考碧云天生产的GSH和GSSG检测试剂盒(S0053)的说明书使用蛋白去除试剂M。

蛋白去除试剂M(S0054)

相关产品请点击如下按钮:
活性氧相关

使用本产品的文献:
1. Liu L,Wang Q,Zhao B,Wu Q,Wang P
Exogenous nicotinamide adenine dinucleotide administration alleviates ischemia/reperfusion-induced oxidative injury in isolated rat hearts via Sirt5-SDH-succinate pathway.
Eur J Pharmacol. 2019 Sep 5;858:172520. (IF 3.263)

谷胱甘肽还原酶检测试剂盒(DTNB法)(S0055)

谷胱甘肽还原酶检测试剂盒(DTNB法)

产品编号: S0055

产品包装:100次
选择包装
100次

说明书下载

486.00 10
价格: ¥ 486.00

产品简介
使用说明
产品图片
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
S0055 谷胱甘肽还原酶检测试剂盒(DTNB法) 100次 486.00元

谷胱甘肽还原酶检测试剂盒(DTNB法)(Glutathione Reductase Assay Kit with DTNB)是一种简单易行的通过比色法来检测细胞、组织或其它样品中谷胱甘肽还原酶(Glutathione reductase,GR)活性的试剂盒。谷胱甘肽还原酶在许多组织中都有分布,可以维持细胞内充足的还原型谷胱甘肽(GSH)水平。GSH可以清除自由基和一些有机过氧化物,或作为谷胱甘肽氧化酶(Glutathione peroxidase)的底物来清除一些过氧化物。
谷胱甘肽还原酶可以还原氧化型谷胱甘肽(GSSG)生成还原型谷胱甘肽(GSH),而GSH可以和生色底物DTNB反应产生黄色的TNB和GSSG,并可以通过测定A412来检测TNB的生成量。适当设置应体系,前后两个反应合并起来后,GSSG/GSH过量而GR相对不足时,该反应体系中谷胱甘肽还原酶就成为整个反应体系的限速因素,此时黄色的TNB生成量和谷胱甘肽还原酶的活性呈线性正相关。从而通过测定A412就可以计算出谷胱甘肽还原酶的活性水平。本试剂盒的具体反应原理如下:

谷胱甘肽还原酶检测试剂盒(DTNB法)(S0055)
谷胱甘肽还原酶检测试剂盒(DTNB法)(S0055)

两个反应相合并:
谷胱甘肽还原酶检测试剂盒(DTNB法)(S0055) 本试剂盒检测肝脏样品的检测效果如图1所示。图中可见,对于肝脏样品可以检测到比较强的谷胱甘肽还原酶的活性并且有很好的剂量效应。
谷胱甘肽还原酶检测试剂盒(DTNB法)(S0055) 图1. 谷胱甘肽还原酶检测试剂盒(DTNB法)用于肝脏裂解样品的实测效果图。 包装清单:

产品编号 产品名称 包装
S0055-1 谷胱甘肽还原酶检测缓冲液 50ml
S0055-2 样品稀释液 50ml
S0055-3 NADPH 5mg
S0055-4 氧化型谷胱甘肽(GSSG) 14.2mg
S0055-5 DTNB 4.5mg
S0055-6 DMSO 1.5ml
说明书 1份

保存条件:
-20℃保存,一年有效。NADPH溶解后宜分装并-70℃保存,4℃可以保存一天,-20℃保存一周后NADPH会降解10%以上。GSSG和DTNB在配制成溶液后,适当分装后-20℃保存。
注意事项:
本试剂盒检测时涉及氧化还原反应,因此所有氧化剂或还原剂都会干扰本试剂盒的测定。如果在样品中的还原剂无法避免,例如DTT、巯基乙醇等,则这些还原剂的总浓度至少低于0.1mM。0.15mM的DTT可以抑制40%的酶活力。另外,硫酸钠、硫酸铵和铁氰化物都会干扰本试剂盒的测定。请尽量避免。
样品可以立即测定,也可以-70℃冻存待以后测定。
一定要严格控制反应时的温度为25℃,否则会引起较多误差。
NADPH不太稳定,要严格按照后续说明操作,谨防失活。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1.样品的准备:
a.细胞样品的准备。对于贴壁细胞,由于后续用于酶活性的测定,应避免使用胰酶消化细胞,可以用PBS、HBSS或生理盐水洗涤一遍。对于悬浮细胞,可以离心收集细胞,并用PBS、HBSS或生理盐水洗涤一遍。后续可以用碧云天生产的Western及IP细胞裂解液(P0013)参考相应的说明裂解细胞样品。按照每100万细胞直接加入100-200微升裂解液的比例进行裂解。对于贴壁细胞,可以使用细胞铲或细胞刮(FLFT021/FSCP023/FSCP029)辅助收集细胞样品。如果出现裂解效果不佳的情况,可以把处在裂解液中的细胞样品用玻璃匀浆器在4℃或冰浴匀浆。随后4℃,12,000×g离心10分钟。取上清用于酶活性的测定。
b.组织样品的准备。动物用含有0.16mg/ml heparin的生理盐水(0.9% NaCl containing 0.16mg/ml heparin)灌流清除血液后获取组织样品。按照约每20mg组织加入200微升Western及IP细胞裂解液(P0013)或其它适当的匀浆液的比例,用TissueMaster™手持式组织研磨仪(E6600)或玻璃匀浆器在4℃或冰浴匀浆。4℃,12,000×g离心10分钟。取上清用于酶活性的测定。
c.红细胞裂解液的准备。用抗凝管收集血液,颠倒混匀。取至少500微升全血4℃ 2500×g离心5分钟。弃上清,用冰冷的约红细胞沉淀10倍体积的Western及IP细胞裂解液(P0013)或其它适当的匀浆液重悬沉淀,再同前离心,弃上清。加入约红细胞沉淀4倍体积的冰冷的Milli-Q级纯水裂解红细胞。12,000×g离心5分钟,取上清。
d.上述各种样品可以用碧云天生产的BCA蛋白浓度测定试剂盒(P0009/P0010/P0010S/P0011/P0012/P0012S)测定蛋白浓度。通常可以先取含1-100微克蛋白的样品用于谷胱甘肽还原酶的检测。注:对于GR活力较高的组织样品,含1-10微克蛋白的样品可能就能满足检测需求,而对于GR活力较低的样品,可能需要10-100微克的蛋白量。如果发现样品中谷胱甘肽还原酶的活力过高,可以用样品稀释液进行稀释。如果样品中谷胱甘肽还原酶的活力过低,则需适当加大蛋白用量。准备好的样品如果当日测定,可以在冰浴或4℃临时保存;如果不能当日检测,可以-70℃冻存。
2.试剂盒的准备工作:
a.配制6mM NADPH溶液的配制。在本试剂盒提供的5mg NADPH中加入1ml Milli-Q级纯水或重蒸水,溶解并混匀,即为6mM NADPH溶液。除立即待用部分外,其余的NADPH溶液需适当分装后尽快-70℃保存。
b.GSSG溶液的配制。在本试剂盒提供的14.2mg GSSG中加入10ml Milli-Q级纯水,溶解并混匀。除立即待用部分外,其余的GSSG溶液需适当分装后-20℃保存。
c.DTNB溶液的配制。在本试剂盒提供的4.5mg DTNB中加入1.5毫升本试剂盒提供的DMSO,溶解并混匀。除立即待用部分外,其余的DTNB溶液需适当分装后-20℃保存。
d.所有试剂在使用前均须在水浴中或PCR仪等设备上温育到25℃。
3.样品测定:
a.参考下表(表1),使用96孔板,依次加入各溶液,混匀。

空白对照 (blank) 样品(sample)
GSSG溶液 100μl 100μl
谷胱甘肽还原酶检测缓冲液 90μl 70-90μl
样品 0μl 0-20μl
NADPH溶液(6mM) 10μl 10μl
总体积 200μl 200μl

b.加入6.6微升DTNB溶液,混匀。
c.立即使用适当的酶标仪或微量紫外分光光度计测定A412,此时记录为0分钟读值,即A412 (Time 0)。如果仪器可以设置温度,把温度设置在25℃,否则可以通过空调调节室温到25℃,待预计仪器也达到25℃后再开始测定A412
d.每隔2分钟记录A412值,即A412 (Time n),至少连续记录10分钟,获得5个点的数据。或者如果仪器具备相应功能,可以让仪器连续测定10分钟或自动每隔2分钟测定一次A412。如果样品的吸光度比较低,可以延长孵育时间,样品的吸光度在一定范围内会随时间的延长接近于线性增加的,此时可以考虑每10分钟测定一次吸光度。
4.样品中谷胱甘肽还原酶酶活力的计算:
a.谷胱甘肽还原酶酶活力单位的定义:1个酶活力单位(1 unit)在25℃,pH7.5的条件下,在1分钟内可以还原化1微摩尔GSSG。1 U=1000 mU。
b.计算时每个样品的A412/min都需要扣除相应的样品本底对照测定出来的A412/min。测定出来的ΔA412/min最好能控制在0.005-0.1范围内。如测定出来的ΔA412/min数值过大,则可以把样品适当稀释或者减小样品的用量,如ΔA412/min数值过小,处理样品时需设法尽量浓缩样品、并适当加大样品的用量。肝脏裂解样品的实测效果参考图1。
注:ΔA412 (blank)=A412 (blank, Time n)-A412 (blank, Time 0);
ΔA412 (sample)=A412 (sample, Time n)-A412 (sample, Time 0);
ΔA412=ΔA412 (sample)-ΔA412 (blank);
ΔA412/min=ΔA412/n
c.对于谷胱甘肽还原酶:1mU/ml=1nmol TNB/min/ml=(ΔA412/min)/(εμM×L(cm))
即相当于:[检测体系中谷胱甘肽还原酶活力]= (ΔA412/min)/(εμM×L(cm))=[(ΔA412 (sample)-ΔA412 (blank))/min]/ (εμM×L(cm))
[样品中谷胱甘肽还原酶活力]=[检测体系中消耗谷胱甘肽还原酶活力]×[稀释倍数]/[样品中的蛋白浓度] = [(ΔA412/min)/(εμM×L(cm))]×[dil×(V(ml)/Vsample(ml))]/[样品中的蛋白浓度]
注:[检测体系中谷胱甘肽还原酶活力]的单位为mU/ml,[样品中的蛋白浓度]的单位为mg/ml,所以最终[样品中谷胱甘肽还原酶活力]的单位为:U/mg蛋白或mU/mg蛋白;
εμM为摩尔消光系数:TNB在A412的摩尔消光系数为0.01415μM-1cm-1
L(cm)为测吸光度时的路径长度:200μl样品在一般的96孔中的高度约为0.552cm,如果使用不同的反应孔,请注意修改为溶液在该孔中的高度;
dil为样品的稀释倍数;
V(ml)为反应体系,本反应体系为0.2ml;
Vsample(ml)为反应体系中样品的体积,以毫升表示。
d.计算示例:样品的蛋白浓度经测定为5mg/ml,用样品稀释液稀释10倍后,取10微升稀释后的样品参考表1进行测定。如果ΔA412/min(sample)=0.048,ΔA412/min(blank)=0.006,那么:
[检测体系中谷胱甘肽还原酶活力]= (0.048-0.006)/(0.01415×0.552) =5.38mU/ml
[样品中谷胱甘肽还原酶活力]= 5.38mU/ml×10×0.2/0.01/(5mg/ml)= 0.2152U/mg(蛋白)

谷胱甘肽还原酶检测试剂盒(DTNB法)(S0055)

相关产品请点击如下按钮:
活性氧相关

使用本产品的文献:
1. Jin WS, Kong ZL, Shen ZF, Jin YZ, Zhang WK, Chen GF.
Regulation of hypoxia inducible factor-1α expression by the alteration of redox status in HepG2cells.
J EXP CLIN CANC RES . 2011 May 19;30:61. (IF 7.068)

2. Min L, He S, Chen Q, Peng F, Peng H, Xie M.
Comparative proteomic analysis of cellular response of human airway epithelial cells (A549) tobenzo(a)pyrene.
TOXICOL MECH METHOD . 2011 Jun;21(5):374-82. (IF 2.295)

3. Zhang H, Chen T, Jiang J, Wong YS, Yang F, Zheng W.
Selenium-containing allophycocyanin purified from selenium-enriched Spirulina of platensisattenuates AAPH-induced oxidative stress in human erythrocytes through inhibition ROS generation.
J AGR FOOD CHEM . 2011 Aug 24;59(16):8683-90. (IF 4.192)

4. Huang HC, Xu, K, jIang ZF.
Curcumin-Mediated Neuroprotection Against Amyloid-β-Induced Mitochondrial Dysfunction Involves the Inhibition of GSK-3β.
J ALZHEIMERS DIS . 2012;32(4):981-96. (IF 3.909)

5. Shi H, Wang Y, Cheng Z, Ye T, Chan Z.
Analysis of natural variation in bermudagrass (Cynodon dactylon) reveals physiological responses underlyingdrought tolerance.
PLoS One . 2012;7(12):e53422. (IF 2.74)

6. Yan F, Fang F, Jin H and Jin Y.
Influence of Tartary Buckwheat Extracts Supplementation on Oxidative Stress Induced by Acute Exhaustive Exercise in Rats.
International Journal of Pharmacology . 2012 Feb;56:173-8. (IF 0.754)

7. Wang L, Hao J, Hu J, Pu J, Lü Z, Zhao L, Wang Q, Yu Q, Wang Y, Li G.
Protective Effects of Ginsenosides against Bisphenol A-Induced Cytotoxicity in 15P-1 Sertoli Cellsvia Extracellular Signal-Regulated Kinase 1/2 Signalling and Antioxidant Mechanisms.
BASIC CLIN PHARMACOL . 2012 Jul;111(1):42-9. (IF 2.651)

8. Wang L, Hao J, Hu J, Pu J, Lü Z, Zhao L, Wang Q, Yu Q, Wang Y, Li G.
Protective effects of ginsenosides against Bisphenol A-induced cytotoxicity in 15P-1 Sertoli cells via extracellular signal-regulated kinase 1/2 signalling and antioxidant mechanisms.
BASIC CLIN PHARMACOL . 2012 Jul;111(1):42-9. (IF 2.651)

9. Lu H, Wan J, Jiang R, Xie J, Peng X, Zhang L.
Sodium butyrate potentiates carbon tetrachloride-induced acute liver injury in mice.
TOXICOL MECH METHOD . 2012 Oct;22(8):648-55. (IF 2.295)

10. Li CW, Wu XL, Zhao XN, Su ZQ, Chen HM, Wang XF, Zhang XJ, Zeng HF, Chen JN, Li YC, Su ZR.
Anti-inflammatory property of the ethanol extract of the root and rhizome of Pogostemon cablin (Blanco) Benth.
THESCIENTIFICWORLDJO . 2013 Dec 9;2013:434151. (IF 1.219)

11. Jiang D, Chen K, Lu X, Gao HJ, Qin ZH, Lin F.
Exercise ameliorates the detrimental effect of chloroquine on skeletal muscles in mice via restoring autophagy flux.
Acta Pharmacol Sin . 2014 Jan;35(1):135-42. (IF 5.064)

12. Chen H, Zha J, Liang X, Li J, Wang Z.
Effects of the human antiepileptic drug carbamazepine on the behavior, biomarkers, and heat shock proteins in the Asian clam Corbicula fluminea.
Aquat Toxicol . 2014 Oct;155:1-8. (IF 4.344)

13. Luo C, Li Y, Yang L, Wang X, Long J, Liu J.
Superparamagnetic iron oxide nanoparticles exacerbate the risks of reactive oxygen species-mediated external stresses.
Arch Toxicol . 2015 Mar;89(3):357-69. (IF 5.059)

14. Zhang G, Zhu J, Zhou Y, Wei Y, Xi L, Qin H, Rao Z, Han M, Ma Y, Wu X.
Hesperidin Alleviates Oxidative Stress and Upregulates the Multidrug Resistance Protein 2 in Isoniazid andRifampicin-Induced Liver Injury in Rats.
J BIOCHEM MOL TOXIC . 2016 Jul;30(7):342-9. (IF 3.606)

15. Ren A, Liu R, Miao ZG, Zhang X, Cao PF, Chen TX, Li CY, Shi L, Jiang AL, Zhao MW.
Hydrogen-rich water regulates effects of ROS balance on morphology, growth and secondarymetabolism via glutathione peroxidase in Ganoderma lucidum.
Environ Microbiol . 2017 Feb;19(2):566-583. (IF 4.933)

16. Zhu Z, Chen Y, Shi G, Zhang X.
Selenium delays tomato fruit ripening by inhibiting ethylene biosynthesis and enhancing the antioxidantdefense system.
Food Chem . 2017 Mar 15;219:179-184. (IF 6.306)

17. Zhu Z, Chen Y, Shi G, Zhang X.
Selenium delays tomato fruit ripening by inhibiting ethylene biosynthesis and enhancing the antioxidant defense system.
Food Chem . 2017 Mar 15;219:179-184. (IF 6.306)

18. He JH, Yan M, Zuo H, Niu S, Yuan J, Weng SP, He J, Xu X.
High reduced/oxidized glutathione ratio in infectious spleen and kidney necrosis virus-infectedcells contributes to degradation of VP08R multimers.
Vet Microbiol . 2017 Aug;207:19-24. (IF 3.03)

19. Zhang S, Shao Q, Geng H, Su S.
The effect of royal jelly on the growth of breast cancer in mice.
Oncol Lett . 2017 Dec;14(6):7615-7621. (IF 2.311)

20. Dai G,Zheng D,Guo W,Yang J,Cheng AY
Cinobufagin Induces Apoptosis in Osteosarcoma Cells Via the Mitochondria-Mediated Apoptotic Pathway.
CELL PHYSIOL BIOCHEM . 2018;46(3):1134-1147. (IF 5.5)

21. Xu Y,Sun D,Song C,Wang R,Dong X
MnTMPyP inhibits paraquat-induced pulmonary epithelial-like cell injury by inhibiting oxidative stress.
J Toxicol Sci . 2018;43(9):545-555. (IF 1.737)

22. Dai J,Gu L,Su Y,Wang Q,Zhao Y,Chen X,Deng H,Li W,Wang G,Li K
Inhibition of curcumin on influenza A virus infection and influenzal pneumonia via oxidative stress, TLR2/4, p38/JNK MAPK and NF-κB pathways.
Int Immunopharmacol . 2018 Jan;54:177-187. (IF 3.943)

23. Duan G, Shi M, Xie L, Xu M, Wang Y, Yan H, Zhuge Y, Zou X.
Increased Glutamine Consumption in Cisplatin-Resistant Cells Has a Negative Impact on CellGrowth.
SCI REP-UK . 2018 Mar 6;8(1):4067. (IF 3.998)

24. Duan G,Shi M,Xie L,Xu M,Wang Y,Yan H,Zhuge Y,Zou X
Increased Glutamine Consumption in Cisplatin-Resistant Cells Has a Negative Impact on Cell Growth.
SCI REP-UK . 2018 Mar 6;8(1):4067. (IF 3.998)

25. Wang J,Zhang Y
Neuroprotective effect of berberine agonist against impairment of learning and memory skills in severe traumatic brain injury via Sirt1/p38 MAPK expression.
Mol Med Rep . 2018 May;17(5):6881-6886. (IF 2.1)

26. Wu D,Han R,Deng S,Liu T,Zhang T,Xie H,Xu Y
Protective Effects of Flagellin A N/C Against Radiation-Induced NLR Pyrin Domain Containing 3 Inflammasome-Dependent Pyroptosis in Intestinal Cells.
INT J RADIAT ONCOL . 2018 May 1;101(1):107-117. (IF 5.859)

27. Lu C,Chen X,Wang Q,Xu X,Xu B
TNFα promotes glioblastoma A172 cell mitochondrial apoptosis via augmenting mitochondrial fission and repression of MAPK-ERK-YAP signaling pathways.
ONCOTARGETS THER . 2018 Oct 18;11:7213-7227. (IF 3.337)

28. Wang Y,An W,Zhang F,Niu M,Liu Y,Shi R
Nebivolol ameliorated kidney damage in Zucker diabetic fatty rats by regulation of oxidative stress/NO pathway: Comparison with captopril.
CLIN EXP PHARMACOL P  . 2018 Nov;45(11):1135-1148. (IF 2.092)

29. Yao S,Yan W
Overexpression of Mst1 reduces gastric cancer cell viability by repressing the AMPK-Sirt3 pathway and activating mitochondrial fission.
ONCOTARGETS THER . 2018 Nov 29;11:8465-8479. (IF 3.337)

30. Zhang X,Högy P,Wu X,Schmid I,Wang X,Schulze WX,Jiang D,Fangmeier A
Physiological and Proteomic Evidence for the Interactive Effects of Post-Anthesis Heat Stress and Elevated CO2 on Wheat.
Proteomics . 2018 Dec;18(23):e1800262. (IF 3.254)

31. Xu S,Zhao X,Liu F,Cao Y,Wang B,Wang X,Yin M,Wang Q,Feng X
Crucial role of oxidative stress in bactericidal effect of parthenolide against Xanthomonas oryzae pv. oryzae.
Pest Manag Sci . 2018 Dec;74(12):2716-2723. (IF 3.75)

32. Xie Z,Zhou Y,Duan X,Yang L
Inhibitory effect of Tanshinone IIA on inverted formin-2 protects HaCaT cells against oxidative injury via regulating mitochondrial stress.
J RECEPT SIG TRANSD. 2019 Apr;39(2):134-145. (IF 1.466)

33. Zhang X,Li F,Cui Y,Liu S,Sun H
Mst1 overexpression combined with Yap knockdown augments thyroid carcinoma apoptosis via promoting MIEF1-related mitochondrial fission and activating the JNK pathway.
Cancer Cell Int. 2019 May 22;19:143. (IF 4.175)

34. Zhang L,Li S,Wang R,Chen C,Ma W,Cai H
Anti-tumor effect of LATS2 on liver cancer death: Role of DRP1-mediated mitochondrial division and the Wnt/β-catenin pathway.
Biomed Pharmacother. 2019 Jun;114:108825. (IF 4.545)

35. Zhang J,Sun L,Li W,Wang Y,Li X,Liu Y
Overexpression of macrophage stimulating 1 enhances the anti-tumor effects of IL-24 in esophageal cancer via inhibiting ERK-Mfn2 signaling-dependent mitophagy.
Biomed Pharmacother. 2019 Jun;114:108844. (IF 4.545)

36. Mao M,Zhang T,Wang Z,Wang H,Xu J,Yin F,Wang G,Sun M,Wang Z,Hua Y,Cai Z
Glaucocalyxin A-induced oxidative stress inhibits the activation of STAT3 signaling pathway and suppresses osteosarcoma progression in vitro and in vivo.
BBA-MOL BASIS DIS. 2019 Jun 1;1865(6):1214-1225. (IF 4.352)

37. Xu X,Huang L,Zhang Z,Tong J,Mi J,Wu Y,Zhang C,Yan H
Targeting non-oncogene ROS pathway by alantolactone in B cell acute lymphoblastic leukemia cells.
Life Sci. 2019 Jun 15;227:153-165. (IF 3.647)

38. Yangyang Chen,Jichun Tang,Yinghong Zhang,Juan Du,Yuanli Wang,Hui Yu,Yanling He
Astaxanthin alleviates gestational diabetes mellitus in mice through suppression of oxidative stress
Naunyn Schmiedebergs Arch Pharmacol. 2020 Dec;393(12):2517-2527.;doi: 10.1007/s00210-020-01861-x.

谷胱甘肽过氧化物酶检测试剂盒(S0056)

谷胱甘肽过氧化物酶检测试剂盒

产品编号: S0056

产品包装:100次
选择包装
100次

说明书下载

488.00 10
价格: ¥ 488.00

产品简介
使用说明
产品图片
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
S0056 谷胱甘肽过氧化物酶检测试剂盒 100次 488.00元

谷胱甘肽过氧化物酶检测试剂盒(NADPH法)(Cellular Glutathione Peroxidase Assay Kit with NADPH)是一种简单易行的通过紫外比色来检测细胞、组织或其它样品中谷胱甘肽过氧化物酶(Glutathione peroxidase, GPx)活性的试剂盒。
绝大部分细胞内的谷胱甘肽过氧化物酶都是含硒的,且硒为该酶的活性中心组成部分。细胞内也有很少量的不含硒的谷胱甘肽过氧化物酶存在。本试剂盒检测的是最常见的含硒的谷胱甘肽过氧化物酶。
本试剂盒的使用灵活方便,样品用量和检测时间的范围宽,样品用量可以根据样品中GPx的活力在0.1-50微升之间调整,检测时间可以根据样品中GPx的活力在5-30分钟内进行调整。
本试剂盒和总谷胱甘肽过氧化物酶检测试剂盒(S0058)配合使用,可以定量检测出样品中不含硒的谷胱甘肽过氧化物酶。
谷胱甘肽过氧化物酶可以清除活细胞内的过氧化物,在保护细胞免受自由基损伤过程中起着关键作用。细胞内的脂类容易和自由基发生反应,产生脂类过氧化物。谷胱甘肽过氧化物酶可以利用还原型谷胱甘肽(GSH)还原脂类过氧化物,从而消除自由基的毒害作用。谷胱甘肽过氧化物酶几乎在所有组织中都有分布。在一些病理状况下谷胱甘肽过氧化物酶的活力会发生明显上调或下调。
谷胱甘肽过氧化物酶可以利用还原型谷胱甘肽(GSH)催化过氧化氢以及许多有机过氧化物,产生水或有机醇。但以过氧化氢为底物进行检测会受同样可以分解过氧化氢的过氧化氢酶(Catalase)的影响,因为过氧化氢酶的酶活性会干扰谷胱甘肽过氧化物酶的测定。本试剂盒利用了一种间接测定的方法。谷胱甘肽过氧化物酶(GPx)可以催化GSH产生GSSG,而谷胱甘肽还原酶可以利用NADPH催化GSSG产生GSH,通过在A340检测NADPH的减少量就可以计算出谷胱甘肽过氧化物酶的活力水平。在上述反应中,谷胱甘肽过氧化物酶是整个反应体系的限速步骤,因此NADPH的减少量和谷胱甘肽过氧化物酶的活力线性相关。
本试剂盒利用了如下的反应原理,GPx为谷胱甘肽过氧化物酶(Glutathione peroxidase),GR为谷胱甘肽还原酶(Glutathione reductase),R-OOH为过氧化物。

谷胱甘肽过氧化物酶检测试剂盒(S0056)
谷胱甘肽过氧化物酶检测试剂盒(S0056)

本试剂盒提供的有机过氧化物试剂(t-Bu-OOH)在没有谷胱甘肽过氧化物酶存在的情况下不会和GSH产生反应,也不会被细胞内的过氧化氢酶催化而分解。因而可以较为特异地检测出谷胱甘肽过氧化物酶的活力。
由于t-Bu-OOH不能被不含硒的谷胱甘肽过氧化物酶所催化,所以本试剂盒可以比较特异地定量检测最常见的含硒的谷胱甘肽过氧化物酶。
本试剂盒可检测组织匀浆产物、细胞裂解产物等样品中谷胱甘肽过氧化物酶的活性。一个试剂盒可进行100次检测。
包装清单:

产品编号 产品名称 包装
S0056-1 样品匀浆液 100ml
S0056-2 谷胱甘肽过氧化物酶检测缓冲液 50ml
S0056-3 谷胱甘肽还原酶 100μl
S0056-4 NADPH 11.5mg
S0056-5 还原型谷胱甘肽(GSH) 10mg
S0056-6 过氧化物试剂(t-Bu-OOH) 200μl
说明书 1份

保存条件:
-20℃保存,一年有效。NADPH溶解后宜适当分装并-70℃保存,4℃可以保存一天,-20℃保存一周后NADPH会降解10%以上。GSH配制成溶液后,适当分装后-20℃保存。
注意事项:
本试剂盒检测时涉及氧化还原反应,因此所有氧化剂或还原剂都会干扰本试剂盒的测定。如果在样品中的还原剂无法避免,例如DTT、巯基乙醇等,则这些还原剂的总浓度至少低于0.1mM。0.15mM的DTT可以抑制40%的酶活力。
常用的Triton X-100、Tween-20等去垢剂都含有较高水平的过氧化物,会影响本试剂盒的测定。如果必须使用这些去垢剂,最好使用纯度较高并注明含较低过氧化物的去垢剂。
加入过氧化物试剂溶液后的第一次读数,例如0分钟时A340读值不宜低于1。如果读数低于1,说明样品中的GPx活力太高,或者样品中本身的GSSG含量太高,需要将样品适当稀释或者减少样品的用量,否则反应很快就进入平台期,无法获取正常的检测数据。样品可以立即测定,也可以-70℃冻存待以后测定。
一定要严格控制反应时的温度为25℃,否则会引起较多误差。
NADPH不太稳定,要严格按照后续说明操作,谨防失活。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1.样品的准备:
a.细胞样品的准备:对于贴壁细胞,由于后续用于酶活性的测定,应避免使用胰酶消化细胞。可以使用EDTA处理细胞或用细胞铲或细胞刮(FLFT021/FSCP023/FSCP029)收集细胞。细胞用PBS或生理盐水洗涤一遍。后续(a)和(b)步骤可以任选其一(优先推荐步骤(a)):
(a).可以用碧云天生产的Western及IP细胞裂解液(P0013)参考相应说明裂解细胞样品。按照每100万细胞加入100-200微升裂解液的比例进行裂解。如果出现裂解效果不佳的情况,可以把处在裂解液中的细胞样品用玻璃匀浆器在4℃或冰浴匀浆。随后4℃,12000g离心10分钟。取上清用于酶活性的测定。
(b).可以用本试剂盒中的样品匀浆液,按照每100万细胞加入100-200微升样品匀浆液的比例用玻璃匀浆器在4℃或冰浴匀浆。随后4℃,12,000g离心10分钟。取上清用于酶活性的测定。
b.组织样品的准备:动物用含有0.16mg/ml heparin的生理盐水(0.9% NaCl containing 0.16mg/ml heparin)灌流清除血液后获取组织样品。按照约每20mg组织加入200微升样品匀浆液的比例,用TissueMaster™手持式组织研磨仪(E6600)或玻璃匀浆器在4℃或冰浴匀浆。4℃,12,000g离心10分钟。取上清用于酶活性的测定。
c.红细胞裂解液的准备:用抗凝管收集血液,颠倒混匀。取至少500微升全血4℃ 2500g离心5分钟。弃上清,用冰冷的约红细胞沉淀10倍体积的样品匀浆液重悬沉淀,再同前离心,弃上清。加入约红细胞沉淀4倍体积的冰冷的Milli-Q级纯水裂解红细胞。12,000g离心5分钟,取上清。
d.上述各种样品可以用碧云天生产的BCA蛋白浓度测定试剂盒(P0009/P0010/P0010S/P0011/P0012/P0012S)测定蛋白浓度。通常可以先取含1-100微克蛋白的样品用于谷胱甘肽过氧化物酶的检测。注:对于GPx活力较高的组织样品,含1-10微克蛋白的样品可能就能满足检测需求,而对于GPx活力较低的样品例如某些细胞样品,可能需要10-100微克的蛋白量。如果发现样品中谷胱甘肽过氧化物酶的活力过高,可以用谷胱甘肽过氧化物酶检测缓冲液进行稀释。如果样品中谷胱甘肽过氧化物酶的活力过低,则需适当加大蛋白用量。准备好的样品如果当日测定,可以在冰浴保存,如果日后测定可以-70℃冻存。
2.试剂盒的准备工作:
a.62.5mM NADPH溶液的配制。在本试剂盒提供的11.5mg NADPH中加入220微升Milli-Q级纯水,溶解并混匀,即为62.5mM NADPH溶液。除立即待用部分外,其余的NADPH溶液需适当分装后-70℃保存。
b.75mM GSH溶液的配制。在本试剂盒提供的10mg GSH中加入433微升Milli-Q级纯水,溶解并混匀,即为75mM GSH溶液。除立即待用部分外,其余的GSH溶液需适当分装后-20℃保存。
c.GPx检测工作液的配制。根据待测定的样品数(含对照),按照每个检测需要40微升GPx检测工作液的体积配制适量的GPx检测工作液。配制好的GPx检测工作液仅限当日使用,且需尽量在冰浴上存放。具体配制方法参考下表。

可测定样品数(含对照) 1个样品 10个样品 20个样品
谷胱甘肽过氧化物酶检测缓冲液 35 µl 350 µl 700 µl
62.5mM NADPH 2 µl 20 µl 40 µl
75mM GSH 2 µl 20 µl 40 µl
谷胱甘肽还原酶 1 µl 10 µl 20 µl
GPx检测工作液 40 µl 400 µl 800 µl

d.30mM过氧化物试剂溶液的配制。取21.5微升过氧化物试剂(t-Bu-OOH)加入5毫升Milli-Q级纯水,混匀,即配制成30mM过氧化物试剂溶液。配制好的30mM过氧化物试剂溶液仅限当日使用,且需尽量在冰浴上存放。
e.所有试剂使用前须在水浴中或PCR仪等设备上温育到25℃。
3. 样品测定:
a.参考下表,使用96孔板,依次加入检测缓冲液、待测样品和GPx检测工作液,混匀,加入40微升GPx检测工作液后,室温孵育15分钟,以消耗掉样品中的GSSG,排除对后继检测的干扰。

空白对照 (blank) 样品 (sample)
谷胱甘肽过氧化物酶检测缓冲液 50 µl 0-50 µl
待测样品 0-50 µl
GPx检测工作液 40 µl 40 µl
总体积 90 µl 90 µl

b.每孔加入10微升30mM过氧化物试剂溶液,混匀。
c.立即使用适当的酶标仪或微量紫外分光光度计测定A340,此时记录为0分钟读值。如果仪器可以设置温度,把温度设置在25℃,否则可以通过空调调节室温到25℃,待预计仪器也达到25℃后再开始测定A340
d.连续测定5分钟或自动每隔1分钟测定一次A340。如果仪器不具备相应功能,可以手工操作,每隔1分钟记录A340值,至少连续记录5分钟,获得6个点的数据。
注1:连续测定的时间可以根据样品中GPx的活力来调整,但是需确保获得6个点的数据。对于GPx的活力较高的样品,例如肝脏等组织样品,建议测定5分钟或10分钟,对应的测定间隔时间设为1分钟和2分钟;对于GPx的活力很低的样品,例如THP-1等细胞样品,可以延长测定时间为10、15或者20分钟,对应的测定间隔时间设为2、3或4分钟。也可以连续测定20分钟,每隔1分钟测定1次,最后取呈线性的时间点前的数据用于分析。
注2:如果样品的第一次读数比如0分钟时A340读值低于1,说明样品中的GPx活力太高,或者样品中本身的GSSG含量太高,需要将样品适当稀释或者减少样品的用量。
e.测定出来的ΔA340/min最好能控制在0.01-0.2范围内。如测定出来的ΔA340/min数值过大,则可以把样品适当稀释或者减小样品的用量,如ΔA340/min数值过小,处理样品时需设法尽量浓缩样品、并适当加大样品的用量。蛋白量为12微克的THP-1细胞样品和蛋白量为4微克的小鼠肝脏样品的检测效果参考表1、表2和图1。
表1.蛋白量为12微克的THP-1细胞样品检测数据分析。

读值 0min 3min 6min 9min 12min 15min
A340 (blank) 1.75 1.63 1.57 1.50 1.42 1.35
A340 (sample) 1.53 1.34 1.21 1.07 0.94 0.81
ΔA340 (blank) 0 0.12 0.18 0.25 0.33 0.40
ΔA340 (sample) 0 0.19 0.32 0.46 0.59 0.72
ΔA340 0 0.07 0.14 0.21 0.26 0.32
ΔA340/min 0 0.023 0.023 0.023 0.0217 0.0213

表2.蛋白量为4微克的小鼠肝脏样品检测数据分析。

读值 0min 1min 2min 3min 4min 5min
A340 (blank) 1.66 1.65 1.64 1.61 1.52 1.49
A340 (sample) 1.49 1.32 1.17 0.99 0.80 0.59
ΔA340 (blank) 0 0.01 0.02 0.05 0.14 0.17
ΔA340 (sample) 0 0.17 0.32 0.50 0.69 0.90
ΔA340 0 0.16 0.30 0.45 0.55 0.73
ΔA340/min 0 0.16 0.15 0.15 0.1375 0.146

注:ΔA340 (blank) = A340 (blank) (Time 0)-A340 (blank) (Time n)
ΔA340 (sample) = A340 (sample) (Time 0)-A340 (sample) (Time n)
ΔA340 = ΔA340 (sample)-ΔA340 (blank)
ΔA340/min =ΔA340/n

谷胱甘肽过氧化物酶检测试剂盒(S0056)

图1.本试剂盒用于THP-1细胞样品和小鼠肝脏样品的检测效果图。横坐标为测定的各个时间点n,纵坐标为相应时间点ΔA340 (sample)与ΔA340 (blank)的差值即ΔA340。图A为12微克THP-1细胞样品测定15分钟的检测效果,图B为4微克小鼠肝脏样品测定5分钟的检测效果。实测数据会因实验条件、检测仪器等的不同而存在差异,图中数据仅供参考。
4.样品中谷胱甘肽过氧化物酶酶活力的计算:
a.谷胱甘肽过氧化物酶酶活力单位的定义:1个酶活力单位(1 unit)在25℃,pH8.0,在GSH、谷胱甘肽还原酶、t-Bu-OOH存在的条件下,在1分钟内可以催化1微摩尔NADPH转变成NADP+。1 U=1000 mU。
b.对于谷胱甘肽过氧化物酶溶液:1mU/ml=1nmol NADPH/min/ml=(ΔA340/min)/(εμM×L(cm))
即相当于:[检测体系中谷胱甘肽过氧化物酶活力]=(ΔA340/min)/(εμM×L(cm))=[(ΔA340 (sample)-ΔA340 (blank))/min]/ (εμM×L(cm))
[样品中谷胱甘肽过氧化物酶活力]=[检测体系中谷胱甘肽过氧化物酶活力]×[稀释倍数]/[样品中的蛋白浓度]= [(ΔA340/min)/(εμM×L(cm))]×[dil×(V(ml)/Vsample(ml))]/[样品中的蛋白浓度]
注:[检测体系中谷胱甘肽过氧化物酶活力]的单位为mU/ml,[样品中的蛋白浓度]的单位为mg/ml,所以最终[样品中谷胱甘肽过氧化物酶活力]的单位为:U/mg蛋白或mU/mg蛋白;
εμM为摩尔消光系数:NADPH在A340的摩尔消光系数为0.00622μM-1cm-1
L(cm)为测吸光度时的路径长度:100μl样品在一般的96孔中的高度约为0.276cm,如果使用不同的反应孔,请注意修改为溶液在该孔中的高度;
dil为样品的稀释倍数;
V(ml)为反应体系,本反应体系为0.1ml;
Vsample(ml)为反应体系中样品的体积,以毫升表示。
c.计算示例:样品的蛋白浓度经测定为1.2mg/ml,用样品稀释液稀释2倍后,取20微升稀释后的样品参考表1进行测定,测定时间设为15分钟。如果0分钟时的A340 (sample)=1.53,A340 (blank)=1.75,15分钟时的A340 (sample)=0.81,A340 (blank)=1.35,则ΔA340 (sample)=1.53-0.81=0.72,ΔA340 (blank)=1.75-1.35=0.40,那么:
[检测体系中谷胱甘肽过氧化物酶活力]= [ (0.72-0.40)/15]/(0.00622×0.276)=12.43mU/ml
[样品中谷胱甘肽过氧化物酶活力]= 12.43mU/ml×(2×0.1/0.02)/(1.2mg/ml)=104mU/mg(蛋白)
相关产品

产品编号 产品名称 包装
S0052 总谷胱甘肽检测试剂盒 100次
S0053 GSH和GSSG检测试剂盒 共100次
S0055 谷胱甘肽还原酶检测试剂盒 100次
S0056 谷胱甘肽过氧化物酶检测试剂盒(NADPH法) 100次
S0057 谷胱甘肽过氧化物酶检测试剂盒(DTNB法) 100次
S0058 总谷胱甘肽过氧化物酶检测试剂盒(NADPH法) 100次
S0059 总谷胱甘肽过氧化物酶检测试剂盒(DTNB法) 100次

谷胱甘肽过氧化物酶检测试剂盒(S0056)

相关产品请点击如下按钮:
活性氧相关

使用本产品的文献:
1. Pan Q, Huang K, He K, Lu F.
Effect of different selenium sources and levels on porcine circovirus type 2 replication in vitro.
J TRACE ELEM MED BIO  . 2008;22(2):143-8. (IF 3.755)

2. An R, Dong C, Lei Y, Han L, Li P, Chen J, Wang G, Shi Q, Gao C, Jiang H, Zhou W, Han J,Chu Y, Dong X.
PrP mutants with different numbers of octarepeat sequences are more susceptible to the oxidative stress.
SCI CHINA LIFE SCI . 2008 Jul;51(7):630-9. (IF 4.611)

3. Wang XZ, Liu SS, Sun Y, Wu JY, Zhou YL, Zhang JH.
Beta-cypermethrin impairs reproductive function in male mice by inducing oxidative stress.
Theriogenology . 2009 Sep 15;72(5):599-611. (IF 2.094)

4. Xiao-Long W, Chuan-Ping Y, Kai X, Ou-Jv Q.
Selenoprotein W depletion in vitro might indicate that its main function is not as an antioxidativeenzyme.
BIOCHEMISTRY-MOSCOW+ . 2010 Feb;75(2):201-7. (IF 1.978)

5. Han ZJ, Song G, Cui Y, Xia HF, Ma X.
Oxidative stress is implicated in arsenic-induced neural tube defects in chick embryos.
Int J Dev Neurosci . 2011 Nov;29(7):673-80. (IF 1.911)

6. Chen J, Xiao S, Deng Y, Du X, Yu Z.
Cloning of a novel glutathione S-transferase 3 (GST3) gene and expressionanalysis in pearl oyster,Pinctada martensii.
FISH SHELLFISH IMMUN . 2011 Dec;31(6):823-30. (IF 3.298)

7. Guo C, He Z, Wen L, Zhu L, Lu Y, Deng S, Yang Y, Wei Q, Yuan H.
Cytoprotective effect of trolox against oxidative damage and apoptosis in the NRK-52e cells induced by melamine.
Cell Biol Int . 2012 Feb;36(2):183-8. (IF 2.571)

8. Shao X, Hu Z, Hu C, Bu Q, Yan G, Deng P, Lv L, Wu D, Deng Y, Zhao J, Zhu R, Li Y, Li H,Xu Y, Yang H, Zhao Y, Cen X.
Taurine protects methamphetamine-induced developmental angiogenesis defect throughantioxidant mechanism.
TOXICOL APPL PHARM  . 2012 May 1;260(3):260-70. (IF 3.616)

9. Huang Y, Fang W, Wang Y, Yang W, Xiong B.
Transforming growth factor-β1 induces glutathione peroxidase-1 and protects from H2O2-induced cell death in colon cancer cells via the Smad2/ERK1/2/HIF-1α pathway.
Int J Mol Med . 2012 May;29(5):906-12. (IF 3.098)

10. Sun WH, Liu F, Chen Y, Zhu YC.
Hydrogen sulfide decreases the levels of ROS by inhibiting mitochondrial complex IV and increasing SODactivities in cardiomyocytes under ischemia/reperfusion.
BIOCHEM BIOPH RES CO . 2012 May 4;421(2):164-9. (IF 2.985)

11. Wang L, Hao J, Hu J, Pu J, Lü Z, Zhao L, Wang Q, Yu Q, Wang Y, Li G.
Protective Effects of Ginsenosides against Bisphenol A-Induced Cytotoxicity in 15P-1 Sertoli Cellsvia Extracellular Signal-Regulated Kinase 1/2 Signalling and AntioxidantMechanisms.
BASIC CLIN PHARMACOL . 2012 Jul;111(1):42-9. (IF 2.651)

12. Shi J, Yin N, Xuan LL, Yao CS, Meng AM, Hou Q.
Vam3, a derivative of resveratrol, attenuates cigarette smoke-induced autophagy.
Acta Pharmacol Sin . 2012 Jul;33(7):888-96. (IF 5.064)

13. Zheng J, Yang B, Yu Y, Chen Q, Huang T, Li D.
Ganoderma lucidum polysaccharides exert anti-hyperglycemic effect on streptozotocin-induced diabetic rats through affecting β-cells.
COMB CHEM HIGH T SCR . 2012 Aug;15(7):542-50. (IF 1.195)

14. Zhang H, Zhai Z, Wang Y, Zhang J, Wu H, Wang Y, Li C, Li D, Lu L, Wang X, Chang J,Hou Q, Ju Z, Zhou D, Meng A.
Resveratrol ameliorates ionizing irradiation-induced long-term hematopoietic stem cell injury in mice.
FREE RADICAL BIO MED . 2013 Jan;54:40-50. (IF 6.17)

15. Wu J, Ding T, Sun J.
Neurotoxic potential of iron oxide nanoparticles in the rat brain striatum and hippocampus.
Neurotoxicology . 2013 Jan;34:243-53. (IF 3.105)

16. Wu J, Tu D, Yuan LY, Yuan H, Wen LX.
T-2 toxin exposure induces apoptosis in rat ovarian granulosa cells through oxidative tress.
ENVIRON TOXICOL CHEM . 2013 Apr 11;36(2):493-500. (IF 3.152)

17. Ali S, Huang Z, Li H, Bashir MH, Ren S.
Antioxidant enzyme influences germination, stress tolerance, and virulence of Isaria fumosorosea.
J BASIC MICROB . 2013 Jun;53(6):489-97. (IF 1.909)

18. Li JW, Ning N, Ma YZ, Zhang R, Tan F, Chen NH.
Claulansine F suppresses apoptosis induced by sodium nitroprusside in PC12 cells.
FREE RADICAL RES . 2013 Jul;47(6-7):488-97. (IF 2.839)

19. Yu CH, Liu ZY, Sun W, Li YJ, Zhang DS, Pan RT, Sun ZL.
Effect of Danofloxacin on Reactive Oxygen Species Production, Lipid Peroxidation and Antioxidant EnzymeActivities in Kidney Tubular Epithelial Cell Line, LLC-PK1.
BASIC CLIN PHARMACOL . 2013 Dec;113(6):377-84. (IF 2.651)

20. Lei T, Li H, Fang Z, Lin J, Wang S, Xiao L, Yang F, Liu X, Zhang J, Huang Z, Liao W.
Polysaccharides from Angelica sinensis alleviate neuronal cell injury caused by oxidative stress.
Neural Regen Res . 2014 Feb 1;9(3):260-7. (IF 3.171)

21. Wu T, Tang Q, Yu Z, Gao Z, Hu H, Chen W, Zheng X, Yu T.
Inhibitory effects of sweet cherry anthocyanins on the obesity development in C57BL/6 mice.
Int J Food Sci Nutr . 2014 May;65(3):351-9. (IF 3.483)

22. Sun H, Deng T, Fu J.
Chicken 15-kDa selenoprotein plays important antioxidative function in splenocytes.
Biol Trace Elem Res . 2014 Dec;161(3):288-96. (IF 2.639)

23. Zhang B, Peng X, Li G, Xu Y, Xia X, Wang Q.
Oxidative stress is involved in Patulin induced apoptosis in HEK293 cells.
Toxicon. 2015 Feb;94:1-7. (IF 2.201)

24. Keogh K, Waters SM, Kelly AK, Wylie AR, Kenny DA.
Effect offeed restriction and subsequent re-alimentation on hormones and genes of the somatotropic axis in cattle.
Physiol Genomics . 2015 Jul;47(7):264-73. (IF 2.749)

25. Li C, Shi L, Chen D, Ren A, Gao T, Zhao M.
Functional analysis of the role of glutathione peroxidase (GPx) in the ROS signaling pathway, hyphal branching and the regulation of ganoderic acid biosynthesis in Ganoderma lucidum.
Fungal Genet Biol . 2015 Sep;82:168-80. (IF 3.071)

26. Yuan XH, Fan YY, Yang CR, Gao XR, Zhang LL, Hu Y, Wang YQ, Jun H.
Progesterone amplifies oxidative stress signal and promotes NO production via H2O2 in mouse kidney arterial endothelial cells.
J STEROID BIOCHEM . 2016 Jan;155(Pt A):104-11. (IF 3.813)

27. Wu T, Yin J, Zhang G, Long H, Zheng X.
Mulberry and cherry anthocyanin consumption prevents oxidative stress and inflammation in diet-induced obese mice.
Mol Nutr Food Res . 2016 Mar;60(3):687-94. (IF 5.309)

28. Wu T, Yin J, Zhang G, Long H, Zheng X.
Mulberry and cherry anthocyanin consumption prevents oxidative stress and inflammation in diet-inducedobese mice.
Mol Nutr Food Res . 2016 Mar;60(3):687-94. (IF 5.309)

29. Ren H, Yang Z, Luo C, Zeng H, Li P, Kang JX, Wan JB, He C, Su H.
Enriched Endogenous Omega-3 Fatty Acids in Mice Ameliorate Parenchymal Cell Death After TraumaticBrain Injury.
Mol Neurobiol . 2016 May 11.[Epub ahead of print] (IF 4.5)

30. Zhang G, Zhu J, Zhou Y, Wei Y, Xi L, Qin H, Rao Z, Han M, Ma Y, Wu X.
Hesperidin Alleviates Oxidative Stress and Upregulates the Multidrug Resistance Protein 2 in Isoniazid andRifampicin-Induced Liver Injury in Rats.
J BIOCHEM MOL TOXIC . 2016 Jul;30(7):342-9. (IF 3.606)

31. He Z, Zhang L, Zhuo C, Jin F, Wang Y.
Apoptosis inhibition effect of Dihydromyricetin against UVA-exposed human keratinocyte cell line.
J PHOTOCH PHOTOBIO B . 2016 Aug;161:40-9. (IF 4.383)

32. Xiong Y, Shang B, Xu S, Zhao R, Gou H, Wang C.
Protective effect of Bu-zhong-yi-qi decoction, the water extract of Chinese
RENAL FAILURE . 2016 Sep;38(8):1240-8. (IF 1.985)

33. Gao W, Liang JX, Ma C, Dong JY, Yan Q.
The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse.
Biomed Res Int . 2017;2017:4176170. (IF 2.276)

34. Zhang WY, Niu CJ, Chen BJ, Yuan L.
Antioxidant responses in hibernating Chinese soft-shelled turtle Pelodiscus sinensis hatchlings.
Comp Biochem Physiol A Mol Integr Physiol . 2017 Feb;204:9-16.

35. Hu W, Wang H, Liu Z, Liu Y, Wang R, Luo X, Huang Y.
Neuroprotective effects of lycopene in spinal cord injury in rats via antioxidative and anti-apoptoticpathway.
Neurosci Lett . 2017 Feb 2. pii:S0304-3940(17)30102-7. (IF 2.274)

36. Yan Y, Jiang W, Tan Y, Zou S, Zhang H, Mao F, Gong A, Qian H, Xu W.
hucMSC Exosome-Derived GPX1 Is Required for the Recovery of Hepatic Oxidant Injury.
Mol Ther . 2017 Feb 1;25(2):465-479. (IF 8.986)

37. Zhu Z, Chen Y, Shi G, Zhang X.
Selenium delays tomato fruit ripening by inhibiting ethylene biosynthesis and enhancing the antioxidantdefense system.
Food Chem . 2017 Mar 15;219:179-184. (IF 6.306)

38. Zhu Z, Chen Y, Shi G, Zhang X.
Selenium delays tomato fruit ripening by inhibiting ethylene biosynthesis and enhancing the antioxidant defense system.
Food Chem . 2017 Mar 15;219:179-184. (IF 6.306)

39. Hu W, Wang H, Liu Z, Liu Y, Wang R, Luo X, Huang Y.
Neuroprotective effects of lycopene in spinal cord injury in rats via antioxidative and anti-apoptotic pathway.
Neurosci Lett . 2017 Mar 6;642:107-112. (IF 2.274)

40. Liu Y, Yang H, Wen Y, Li B, Zhao Y, Xing J, Zhang M, Chen Y.
Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress.
Int J Mol Sci . 2017 May 17;18(5). pii: E1076. (IF 4.556)

41. Feng J,Li H,Zhang Y,Wang Q,Zhao S,Meng P,Li J
Mammalian STE20-Like Kinase 1 Deletion Alleviates Renal Ischaemia-Reperfusion Injury via Modulating Mitophagy and the AMPK-YAP Signalling Pathway.
CELL PHYSIOL BIOCHEM . 2018;51(5):2359-2376. (IF 5.5)

42. Zhang Y,Feng J,Wang Q,Zhao S,Yang S,Tian L,Meng P,Li J,Li H
Hyperglycaemia Stress-Induced Renal Injury is Caused by Extensive Mitochondrial Fragmentation, Attenuated MKP1 Signalling, and Activated JNK-CaMKII-Fis1 Biological Axis.
CELL PHYSIOL BIOCHEM . 2018;51(4):1778-1798. (IF 5.5)

43. Chen L,Yao H,Chen X,Wang Z,Xiang Y,Xia J,Liu Y,Wang Y
Ginsenoside Rg1 Decreases Oxidative Stress and Down-Regulates Akt/mTOR Signalling to Attenuate Cognitive Impairment in Mice and Senescence of Neural Stem Cells Induced by D-Galactose.
Neurochem Res . 2018 Feb;43(2):430-440. (IF 3.038)

44. Duan G, Shi M, Xie L, Xu M, Wang Y, Yan H, Zhuge Y, Zou X.
Increased Glutamine Consumption in Cisplatin-Resistant Cells Has a Negative Impact on CellGrowth.
SCI REP-UK . 2018 Mar 6;8(1):4067. (IF 3.998)

45. Zhang N,Feng H,Liao HH,Chen S,Yang Z,Deng W,Tang QZ
Myricetin attenuated LPS induced cardiac injury in vivo and in vitro.
Phytother Res . 2018 Mar;32(3):459-470. (IF 4.087)

46. Ling H,Zhu Z,Yang J,He J,Yang S,Wu D,Feng S,Liao D
Dihydromyricetin improves type 2 diabetes-induced cognitive impairment via suppressing oxidative stress and enhancing brain-derived neurotrophic factor-mediated neuroprotection in mice.
ACTA BIOCH BIOPH SIN . 2018 Mar 1;50(3):298-306. (IF 2.836)

47. Duan G,Shi M,Xie L,Xu M,Wang Y,Yan H,Zhuge Y,Zou X
Increased Glutamine Consumption in Cisplatin-Resistant Cells Has a Negative Impact on Cell Growth.
SCI REP-UK . 2018 Mar 6;8(1):4067. (IF 3.998)

48. Guo J,Xu B,Han Q,Zhou H,Xia Y,Gong C,Dai X,Li Z,Wu G
Ferroptosis: A Novel Anti-tumor Action for Cisplatin.
Cancer Res Treat . 2018 Apr;50(2):445-460. (IF 3.761)

49. Liu R,Zhang X,Ren A,Shi DK,Shi L,Zhu J,Yu HS,Zhao MW
Heat stress-induced reactive oxygen species participate in the regulation of HSP expression, hyphal branching and ganoderic acid biosynthesis in Ganoderma lucidum.
Microbiol Res . 2018 Apr;209:43-54. (IF 3.97)

50. Wu D,Han R,Deng S,Liu T,Zhang T,Xie H,Xu Y
Protective Effects of Flagellin A N/C Against Radiation-Induced NLR Pyrin Domain Containing 3 Inflammasome-Dependent Pyroptosis in Intestinal Cells.
INT J RADIAT ONCOL . 2018 May 1;101(1):107-117. (IF 5.859)

51. Chen S, Fan B.
Myricetin protects cardiomyocytes from LPS-induced injury.
Herz. 2018 May;43(3):265-274. (IF 1.033)

52. Yang Y,Xie F,Qin D,Zong C,Han F,Pu Z,Liu D,Li X,Zhang Y,Liu Y,Wang X
The orphan nuclear receptor NR4A1 attenuates oxidative stress-induced β cells apoptosis via up-regulation of glutathione peroxidase 1.
Life Sci . 2018 Jun 15;203:225-232. (IF 3.647)

53. Yanfei Cui,Shengya Yang
Overexpression of Annexin A1 Protects Against Benzo[a]pyrene‑induced Bronchial Epithelium Injury
Mol Med Rep. 2018 Jul;18(1):349-357.;doi: 10.3892/mmr.2018.8998. (IF 2.1)

54. Ma X,Hu B,Zou C,Han A,Xu Z,Zhang T,Yu W
The effects of hyperoxia liquid regulate cardiopulmonary bypass‑induced myocardial damage through the Nrf2‑ARE signaling pathway.
Mol Med Rep . 2018 Aug;18(2):2342-2348. (IF 2.1)

55. Zhu Z,Xie Q,Huang Y,Zhang S,Chen Y
Aucubin suppresses Titanium particles‑mediated apoptosis of MC3T3‑E1 cells and facilitates osteogenesis by affecting the BMP2/Smads/RunX2 signaling pathway.
Mol Med Rep . 2018 Sep;18(3):2561-2570. (IF 2.1)

56. Huang Y,Hu Z
UBIAD1 protects against oxygen-glucose deprivation/reperfusion-induced multiple subcellular organelles injury through PI3K/AKT pathway in N2A cells.
J Cell Physiol . 2018 Sep;233(9):7480-7496. (IF 5.546)

57. Jieensinue S,Zhu H,Li G,Dong K,Liang M,Li Y
Tanshinone IIA reduces SW837 colorectal cancer cell viability via the promotion of mitochondrial fission by activating JNK-Mff signaling pathways.
BMC Cell Biol . 2018 Sep 25;19(1):21. (IF 3.066)

58. Lu C,Chen X,Wang Q,Xu X,Xu B
TNFα promotes glioblastoma A172 cell mitochondrial apoptosis via augmenting mitochondrial fission and repression of MAPK-ERK-YAP signaling pathways.
ONCOTARGETS THER . 2018 Oct 18;11:7213-7227. (IF 3.337)

59. Wan J,Cui J,Wang L,Wu K,Hong X,Zou Y,Zhao S,Ke H
Excessive mitochondrial fragmentation triggered by erlotinib promotes pancreatic cancer PANC-1 cell apoptosis via activating the mROS-HtrA2/Omi pathways.
Cancer Cell Int . 2018 Oct 22;18:165. (IF 4.175)

60. Liang Y,Huang W,Zeng D,Huang X,Chan L,Mei C,Feng P,Tan CH,Chen T
Cancer-targeted design of bioresponsive prodrug with enhanced cellular uptake to achieve precise cancer therapy.
Drug Deliv . 2018 Nov;25(1):1350-1361. (IF 4.902)

61. Liu J,Xu Y,Wu Q,Ding Q,Fan W
Sirtuin‑1 protects hair follicle stem cells from TNFα-mediated inflammatory stress via activating the MAPK-ERK-Mfn2 pathway.
Life Sci . 2018 Nov 1;212:213-224. (IF 3.647)

62. Tian HP,Sun YH,He L,Yi YF,Gao X,Xu DL
Single-Stranded DNA-Binding Protein 1 Abrogates Cardiac Fibroblast Proliferation and Collagen Expression Induced by Angiotensin II.
Int Heart J . 2018 Nov 28;59(6):1398-1408. (IF 1.906)

63. Yao S,Yan W
Overexpression of Mst1 reduces gastric cancer cell viability by repressing the AMPK-Sirt3 pathway and activating mitochondrial fission.
ONCOTARGETS THER . 2018 Nov 29;11:8465-8479. (IF 3.337)

64. Zhang W,Liu K,Pei Y,Ma J,Tan J,Zhao J
Mst1 regulates non-small cell lung cancer A549 cell apoptosis by inducing mitochondrial damage via ROCK1/F‑actin pathways.
Int J Oncol . 2018 Dec;53(6):2409-2422. (IF 3.899)

65. Liu D,Xue J,Liu Y,Gu H,Wei X,Ma W,Luo W,Ma L,Jia S,Dong N,Huang J,Wang Y,Yuan Z
Inhibition of NRF2 signaling and increased reactive oxygen species during embryogenesis in a rat model of retinoic acid-induced neural tube defects.
Neurotoxicology . 2018 Dec;69:84-92. (IF 3.105)

66. Li DY,Liu WT,Wang GY,Shi XJ
Impact of combined ischemic preconditioning and remote ischemic perconditioning on ischemia-reperfusion injury after liver transplantation.
SCI REP-UK . 2018 Dec 19;8(1):17979. (IF 3.998)

67. Wang S,Li F,Qiao R,Hu X,Liao H,Chen L,Wu J,Wu H,Zhao M,Liu J,Chen R,Ma X,Kim D,Sun J,Davis TP,Chen C,Tian J,Hyeon T,Ling D
Arginine-Rich Manganese Silicate Nanobubbles as a Ferroptosis-Inducing Agent for Tumor-Targeted Theranostics.
ACS Nano . 2018 Dec 26;12(12):12380-12392. (IF 14.588)

68. Liang F,Su F,Wang X,Long S,Zheng Y,He X,Pang J,Pei Z
Xyloketal derivative C53N protects against mild traumatic brain injury in mice.
DRUG DES DEV THER. 2018 Dec 27;13:173-182. (IF 3.216)

69. He M,Xiang Z,Xu L,Duan Y,Li F,Chen J
Lipopolysaccharide induces human olfactory ensheathing glial apoptosis by promoting mitochondrial dysfunction and activating the JNK-Bnip3-Bax pathway.
CELL STRESS CHAPERON . 2019 Jan;24(1):91-104. (IF 2.892)

70. Zhang Y,Zhang J,Wu C,Guo S,Su J,Zhao W,Xing H
Higenamine protects neuronal cells from oxygen-glucose deprivation/reoxygenation-induced injury.
J Cell Biochem . 2019 Mar;120(3):3757-3764. (IF 4.237)

71. Yin H,Qi Z,Li M,Ahammed GJ,Chu X,Zhou J
Selenium forms and methods of application differentially modulate plant growth, photosynthesis, stress tolerance, selenium content and speciation in Oryza sativa L.
ECOTOX ENVIRON SAFE. 2019 Mar;169:911-917. (IF 4.872)

72. Zhang C,Yang Y,Liang W,Wang T,Wang S,Wang X,Wang Y,Jiang H,Feng H
Neuroprotection by urate on the mutant hSOD1-related cellular and Drosophila models of amyotrophic lateral sclerosis: Implication for GSH synthesis via activating Akt/GSK3β/Nrf2/GCLC pathways.
Brain Res Bull. 2019 Mar;146:287-301. (IF 3.37)

73. Xie Z,Zhou Y,Duan X,Yang L
Inhibitory effect of Tanshinone IIA on inverted formin-2 protects HaCaT cells against oxidative injury via regulating mitochondrial stress.
J RECEPT SIG TRANSD. 2019 Apr;39(2):134-145. (IF 1.466)

74. Quan XJ,Liang CL,Sun MZ,Zhang L,Li XL
Overexpression of steroid receptor coactivators alleviates hyperglycemia-induced endothelial cell injury in rats through activating the PI3K/Akt pathway.
Acta Pharmacol Sin . 2019 May;40(5):648-657. (IF 5.064)

75. Zhang L,Li S,Wang R,Chen C,Ma W,Cai H
Anti-tumor effect of LATS2 on liver cancer death: Role of DRP1-mediated mitochondrial division and the Wnt/β-catenin pathway.
Biomed Pharmacother. 2019 Jun;114:108825. (IF 4.545)

76. Zhang J,Sun L,Li W,Wang Y,Li X,Liu Y
Overexpression of macrophage stimulating 1 enhances the anti-tumor effects of IL-24 in esophageal cancer via inhibiting ERK-Mfn2 signaling-dependent mitophagy.
Biomed Pharmacother. 2019 Jun;114:108844. (IF 4.545)

77. Lei M,Wu X,Huang C,Qiu Z,Wang L,Zhang R,Zhang J
Trehalose induced by reactive oxygen species relieved the radial growth defects of Pleurotus ostreatus under heat stress.
APPL MICROBIOL BIOT. 2019 Jul;103(13):5379-5390. (IF 3.53)

78. Li Y,Zhang J,Liu H,Yuan J,Yin Y,Wang T,Cheng B,Sun S,Guo Z
Curcumin ameliorates glyoxylate-induced calcium oxalate deposition and renal injuries in mice.
Phytomedicine. 2019 Aug;61:152861. (IF 4.268)

79. Huang MZ,Yang YJ,Liu XW,Qin Z,Li JY
Aspirin Eugenol Ester Reduces H2O2-Induced Oxidative Stress of HUVECs via Mitochondria-Lysosome Axis.
Oxid Med Cell Longev. 2019 Sep 9;2019:8098135. (IF 5.076)

80. Wang R,Sun DG,Song G,Guan CY,Cui Y,Ma X,Xia HF
Choline, not folate, can attenuate the teratogenic effects ofdibutyl phthalate (DBP) during early chick embryo development.
ENVIRON SCI POLLUT R. 2019 Oct;26(29):29763-29779. (IF 3.056)

81. Zhang J,Zhang SD,Wang P,Guo N,Wang W,Yao LP,Yang Q,Efferth T,Jiao J,Fu YJ
Pinolenic acid ameliorates oleic acid-induced lipogenesis and oxidative stress via AMPK/SIRT1 signaling pathway in HepG2 cells.
Eur J Pharmacol. 2019 Oct 15;861:172618. (IF 3.263)

82. Gao M,Deng J,Liu F,Fan A,Wang Y,Wu H,Ding D,Kong D,Wang Z,Peer D,Zhao Y
Triggered ferroptotic polymer micelles for reversing multidrug resistance to chemotherapy.
Biomaterials. 2019 Dec;223:119486. (IF 10.317)

83. Zhang C,Liang W,Wang H,Yang Y,Wang T,Wang S,Wang X,Wang Y,Feng H
γ-Oryzanol mitigates oxidative stress and prevents mutant SOD1-Related neurotoxicity in Drosophila and cell models of amyotrophic lateral sclerosis.
Neuropharmacology. 2019 Dec 1;160:107777. (IF 4.431)

84. Tang H,Chen D,Li C,Zheng C,Wu X,Zhang Y,Song Q,Fei W
Dual GSH-exhausting sorafenib loaded manganese-silica nanodrugs for inducing the ferroptosis of hepatocellular carcinoma cells.
INT J PHARMACOL. 2019 Dec 15;572:118782. (IF 0.692)

85. Dongdong Zhang,Yujiao Liu,Ziyi Luo,Yanling Chen,Anjie Xu,Yuxing Liang,Balu Wu,Xiqin Tong,Xiaoyan Liu,Hui Shen,Li Liu,Yongchang Wei,Haibing Zhou,Yi Liu,Fuling Zhou
The novel thioredoxin reductase inhibitor A-Z2 triggers intrinsic apoptosis and shows efficacy in the treatment of acute myeloid leukemia
Free Radic Biol Med. 2020 Jan;146:275-286.;doi: 10.1016/j.freeradbiomed.2019.11.013.

86. Baocheng Zhao,Zhenjun Wang,Jiagang Han,Guanghui Wei,Bingqiang Yi,Zhulin Li
Rhizoma Paridis total saponins alleviate H2O2‑induced oxidative stress injury by upregulating the Nrf2 pathway
Mol Med Rep. 2020 Jan;21(1):220-228.;doi: 10.3892/mmr.2019.10827.

87. Xiangyuan Chen,Jie Qi,Qichao Wu,Hui Jiang,Jing Wang,Wankun Chen,Anrong Mao,Minmin Zhu
High glucose inhibits vascular endothelial Keap1/Nrf2/ARE signal pathway via downregulation of monomethyltransferase SET8 expression
Acta Biochim Biophys Sin (Shanghai). 2020 May 26;52(5):506-516.;doi: 10.1093/abbs/gmaa023.

88. Song Zhou,Yunjing Sun,Kai Zhao,Yanzhou Gao,Jiangman Cui,Liping Qi,Lingfang Huang
miR‑21/PTEN pathway mediates the cardioprotection of geniposide against oxidized low‑density lipoprotein‑induced endothelial injury via suppressing oxidative stress and inflammatory response
Int J Mol Med. 2020 May;45(5):1305-1316.;doi: 10.3892/ijmm.2020.4520.

89. Yunhua Yu,Fei Wang,Jianjun Wang,Dongmei Zhang,Xianxian Zhao
Ketogenic diet attenuates aging-associated myocardial remodeling and dysfunction in mice
Exp Gerontol. 2020 Oct 15;140:111058.;doi: 10.1016/j.exger.2020.111058.

90. Zi-Sheng Huang,Da-Qi Xie,Li-Jun Xu,Chang-Shun Huang,Min Zheng,Yi-Jun Chen,Yin Cao
Tetramethylpyrazine Ameliorates Lipopolysaccharide-Induced Sepsis in Rats via Protecting Blood-Brain Barrier, Impairing Inflammation and Nitrous Oxide Systems
Front Pharmacol. 2020 Oct 2;11:562084.;doi: 10.3389/fphar.2020.562084.

总谷胱甘肽过氧化物酶检测试剂盒(S0058)

总谷胱甘肽过氧化物酶检测试剂盒

产品编号: S0058

产品包装:100次
选择包装
100次

说明书下载

556.00 10
价格: ¥ 556.00

产品简介
使用说明
产品图片
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
S0058 总谷胱甘肽过氧化物酶检测试剂盒 100次 556.00元

总谷胱甘肽过氧化物酶检测试剂盒(NADPH法) (Total Glutathione Peroxidase Assay Kit with NADPH)是一种简单易行的通过紫外比色来检测细胞、组织或其它样品中谷胱甘肽过氧化物酶(Glutathione peroxidase, GPx)活性的试剂盒。
绝大部分细胞内的谷胱甘肽过氧化物酶都是含硒的,且硒为该酶的活性中心组成部分。细胞内也有很少量的不含硒的谷胱甘肽过氧化物酶存在。本试剂盒可以定量检测出最常见的含硒的谷胱甘肽过氧化物酶和少量不含硒的谷胱甘肽过氧化物酶的总量。
本试剂盒和谷胱甘肽过氧化物酶检测试剂盒(S0056)配合使用,可以定量检测出样品中不含硒的谷胱甘肽过氧化物酶。
谷胱甘肽过氧化物酶可以清除活细胞内的过氧化物,在保护细胞免受自由基损伤过程中起着关键作用。细胞内的脂类容易和自由基发生反应,产生脂类过氧化物。谷胱甘肽过氧化物酶可以利用还原型谷胱甘肽(GSH)还原脂类过氧化物,从而消除自由基的毒害作用。谷胱甘肽过氧化物酶几乎在所有组织中都有分布。在一些病理状况下谷胱甘肽过氧化物酶的活力会发生明显上调或下调。
谷胱甘肽过氧化物酶可以利用还原型谷胱甘肽(GSH)催化过氧化氢以及许多有机过氧化物,产生水或有机醇。但以过氧化氢为底物进行检测会受同样可以分解过氧化氢的过氧化氢酶(Catalase)的影响,因为过氧化氢酶的酶活性会干扰谷胱甘肽过氧化物酶的测定。本试剂盒利用了一种间接测定的方法。谷胱甘肽过氧化物酶(GPx)可以催化GSH产生GSSG,而谷胱甘肽还原酶可以利用NADPH催化GSSG产生GSH,通过检测NADPH的减少量就可以计算出谷胱甘肽过氧化物酶的活力水平。在上述反应中谷胱甘肽过氧化物酶是整个反应体系的限速步骤,因此NADPH的减少量和谷胱甘肽过氧化物酶的活力线性相关。
本试剂盒利用了如下的反应原理,GPx为谷胱甘肽过氧化物酶(Glutathione peroxidase),GR为谷胱甘肽还原酶(Glutathione reductase),R-OOH为过氧化物。

总谷胱甘肽过氧化物酶检测试剂盒(S0058)
总谷胱甘肽过氧化物酶检测试剂盒(S0058)

本试剂盒提供的有机过氧化物试剂(Cum-OOH)在没有谷胱甘肽过氧化物酶存在的情况下不会和GSH产生反应,也不会被细胞内的过氧化氢酶催化而分解。因而可以较为特异地检测出谷胱甘肽过氧化物酶的活力。
有机过氧化物试剂(Cum-OOH)不仅可以作为含硒的谷胱甘肽过氧化物酶的底物,也可作为不含硒的谷胱甘肽过氧化物酶的底物,因此本试剂盒可以定量检测总谷胱甘肽过氧化物酶。
本试剂盒可检测组织匀浆产物、细胞裂解产物等样品中谷胱甘肽过氧化物酶的活性。一个试剂盒可进行100次检测。
包装清单:

产品编号 产品名称 包装
S0058-1 样品匀浆液 100ml
S0058-2 谷胱甘肽过氧化物酶检测缓冲液 50ml
S0058-3 谷胱甘肽还原酶 100µl
S0058-4 NADPH 11.5mg
S0058-5 还原型谷胱甘肽(GSH) 10mg
S0058-6 过氧化物试剂(Cum-OOH) 200µl
说明书 1份

保存条件:
-20℃保存,一年有效。NADPH溶解后宜适当分装并-70℃保存,4℃可以保存一天,-20℃保存一周后NADPH会降解10%以上。GSH在配制成溶液后,适当分装后-20℃保存。
注意事项:
本试剂盒检测时涉及到氧化还原反应,因此所有氧化剂或还原剂都会干扰本试剂盒的测定。如果在样品中的还原剂无法避免,例如DTT、巯基乙醇等,则这些还原剂的总浓度至少低于0.1mM。0.15mM的DTT可以抑制40%的酶活力。
常用的Triton X-100、Tween-20等去垢剂都含有较高水平的过氧化物,会影响本试剂盒的测定。如果必须使用这些去垢剂,最好使用纯度较高并注明含较低过氧化物的去垢剂。
加入过氧化物试剂溶液后的第一次读数,例如0分钟时A340读值不宜低于1。如果读数低于1,说明样品中的GPx活力太高,或者样品中本身的GSSG含量太高,需要将样品适当稀释或者减少样品的用量,否则反应很快就进入平台期,无法获取正常的检测数据。样品可以立即测定,也可以-70℃冻存待以后测定。
一定要严格控制反应时的温度为25℃,否则会引起较多误差。
NADPH不太稳定,要严格按照后续说明操作,谨防失活。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1.样品的准备:
a.细胞样品的准备:对于贴壁细胞,由于后续用于酶活性的测定,应避免使用胰酶消化细胞。可以使用EDTA处理细胞或用细胞铲或细胞刮(FLFT021/FSCP023/FSCP029)收集细胞。细胞用PBS或生理盐水洗涤一遍。后续(a)和(b)步骤可以任选其一(优先推荐步骤(a)):
(a).可以用碧云天生产的Western及IP细胞裂解液(P0013)参考相应说明裂解细胞样品。按照每100万细胞加入100-200微升裂解液的比例进行裂解。如果出现裂解效果不佳的情况,可以把处在裂解液中的细胞样品用玻璃匀浆器在4℃或冰浴匀浆。随后4℃,12000g离心10分钟。取上清用于酶活性的测定。
(b).可以用本试剂盒中的样品匀浆液,按照每100万细胞加入100-200微升样品匀浆液的比例用玻璃匀浆器在4℃或冰浴匀浆。随后4℃,12,000g离心10分钟。取上清用于酶活性的测定。
b.组织样品的准备:动物用含有0.16mg/ml heparin的生理盐水(0.9% NaCl containing 0.16mg/ml heparin)灌流清除血液后获取组织样品。按照约每20mg组织加入200微升样品匀浆液的比例,用TissueMaster™手持式组织研磨仪(E6600)或玻璃匀浆器在4℃或冰浴匀浆。4℃,12,000g离心10分钟。取上清用于酶活性的测定。
c.红细胞裂解液的准备:用抗凝管收集血液,颠倒混匀。取至少500微升全血4℃ 2500g离心5分钟。弃上清,用冰冷的约红细胞沉淀10倍体积的样品匀浆液重悬沉淀,再同前离心,弃上清。加入约红细胞沉淀4倍体积的冰冷的Milli-Q级纯水裂解红细胞。12,000g离心5分钟,取上清。
d.上述各种样品可以用碧云天生产的BCA蛋白浓度测定试剂盒(P0009/P0010/P0010S/P0011/P0012/P0012S)测定蛋白浓度。通常可以先取含1-100微克蛋白的样品用于谷胱甘肽过氧化物酶的检测。注:对于GPx活力较高的组织样品,含1-10微克蛋白的样品可能就能满足检测需求,而对于GPx活力较低的样品例如某些细胞样品,可能需要10-100微克的蛋白量。如果发现样品中谷胱甘肽过氧化物酶的活力过高,可以用谷胱甘肽过氧化物酶检测缓冲液进行稀释。如果样品中谷胱甘肽过氧化物酶的活力过低,则需适当加大蛋白用量。准备好的样品如果当日测定,可以在冰浴保存,如果日后测定可以-70℃冻存。
2.试剂盒的准备工作:
a.62.5mM NADPH溶液的配制。在本试剂盒提供的11.5mg NADPH中加入220微升Milli-Q级纯水,溶解并混匀,即为62.5mM NADPH溶液。除立即待用部分外,其余的NADPH溶液需适当分装后-70℃保存。
b.75mM GSH溶液的配制。在本试剂盒提供的10mg GSH中加入433微升Milli-Q级纯水,溶解并混匀,即为75mM GSH溶液。除立即待用部分外,其余的GSH溶液需适当分装后-20℃保存。
c.GPx检测工作液的配制。根据待测定的样品数(含对照),按照每个检测需要40微升GPx检测工作液的体积配制适量的GPx检测工作液。配制好的GPx检测工作液仅限当日使用,且需尽量在冰浴上存放。具体配制方法参考下表。

可测定样品数(含对照) 1个样品 10个样品 20个样品
谷胱甘肽过氧化物酶检测缓冲液 35 µl 350 µl 700 µl
62.5mM NADPH 2 µl 20 µl 40 µl
75mM GSH 2 µl 20 µl 40 µl
谷胱甘肽还原酶 1 µl 10 µl 20 µl
GPx检测工作液 40 µl 400 µl 800 µl

d.30mM过氧化物试剂溶液的配制。取21.5微升过氧化物试剂(Cum-OOH)加入5毫升Milli-Q级纯水,混匀,即配制成30mM过氧化物试剂溶液。配制好的30mM过氧化物试剂溶液仅限当日使用,且需尽量在冰浴上存放。
e.所有试剂使用前须在水浴中或PCR仪等设备上温育到25℃。
3.样品测定:
a.参考下表,使用96孔板,依次加入检测缓冲液、待测样品和GPx检测工作液,混匀,加入40微升GPx检测工作液后,室温孵育15分钟,以消耗掉样品中的GSSG,排除对后继检测的干扰。

空白对照 (blank) 样品 (sample)
谷胱甘肽过氧化物酶检测缓冲液 50 µl 0-50 µl
待测样品 0-50 µl
GPx检测工作液 40 µl 40 µl
总体积 90 µl 90 µl

b. 每孔加入10微升30mM过氧化物试剂溶液,混匀。
c.立即使用适当的酶标仪或微量紫外分光光度计测定A340,此时记录为0分钟读值。如果仪器可以设置温度,把温度设置在25℃,否则可以通过空调调节室温到25℃,待预计仪器也达到25℃后再开始测定A340
d.连续测定5分钟或自动每隔1分钟测定一次A340。如果仪器不具备相应功能,可以手工操作,每隔1分钟记录A340值,至少连续记录5分钟,获得6个点的数据。
注1:连续测定的时间可以根据样品中GPx的活力来调整,但是需确保获得6个点的数据。对于GPx的活力较高的样品,例如肝脏等组织样品,建议测定5分钟或10分钟,对应的测定间隔时间设为1分钟和2分钟;对于GPx的活力很低的样品,例如THP-1等细胞样品,可以延长测定时间为10、15或者20分钟,对应的测定间隔时间设为2、3或4分钟。也可以连续测定20分钟,每隔1分钟测定1次,最后取呈线性的时间点前的数据来分析。
注2:如果样品的第一次读数比如0分钟时A340读值低于1,说明样品中的GPx活力太高,或者样品中本身的GSSG含量太高,需要将样品适当稀释或者减少样品的用量。
e.测定出来的ΔA340/min最好能控制在0.01-0.2范围内。如测定出来的ΔA340/min数值过大,则可以把样品适当稀释或者减小样品的用量,如ΔA340/min数值过小,处理样品时需设法尽量浓缩样品、并适当加大样品的用量。蛋白量为12微克的THP-1细胞样品和蛋白量为4微克的小鼠肝脏样品的检测效果参考表1、表2和图1。
表1.蛋白量为12微克的THP-1细胞样品检测数据分析。

读值 0min 3min 6min 9min 12min 15min
A340 (blank) 1.50 1.35 1.22 1.08 0.96 0.86
A340 (sample) 1.44 1.21 0.98 0.72 0.50 0.32
ΔA340 (blank) 0.00 0.15 0.28 0.42 0.54 0.64
ΔA340 (sample) 0.00 0.23 0.46 0.72 0.94 1.12
ΔA340 0.00 0.08 0.18 0.30 0.40 0.48
ΔA340/min 0 0.0267 0.03 0.033 0.033 0.032

表2. 蛋白量为4微克的小鼠肝脏样品的检测数据分析。

读值 0min 1min 2min 3min 4min 5min
A340 (blank) 1.72 1.62 1.57 1.51 1.49 1.46
A340 (sample) 1.37 1.12 0.95 0.79 0.62 0.45
ΔA340 (blank) 0.00 0.10 0.15 0.21 0.23 0.26
ΔA340 (sample) 0.00 0.25 0.41 0.58 0.75 0.92
ΔA340 0.00 0.15 0.26 0.37 0.52 0.66
ΔA340/min 0 0.15 0.13 0.123 0.13 0.132

注:ΔA340 (blank)=A340 (blank) (Time 0)-A340 (blank) (Time n)
ΔA340 (sample)=A340 (sample) (Time 0)-A340 (sample) (Time n)
ΔA340=ΔA340 (sample)-ΔA340 (blank)
ΔA340/min=ΔA340/n

总谷胱甘肽过氧化物酶检测试剂盒(S0058)

图1.本试剂盒用于THP-1细胞样品和小鼠肝脏样品的检测效果图。横坐标为测定的各个时间点n,纵坐标为相应时间点ΔA340 (sample)与ΔA340 (blank)的差值即ΔA340。图A为蛋白量为12微克的THP-1细胞样品测定15分钟的检测效果,图B为蛋白量为4微克的小鼠肝脏样品测定5分钟的检测效果。实测数据会因实验条件、检测仪器等的不同而存在差异,图中数据仅供参考。
4.样品中谷胱甘肽过氧化物酶酶活力的计算:
a.谷胱甘肽过氧化物酶酶活力单位的定义:1个酶活力单位(1 unit)在25℃,pH8.0,在GSH、谷胱甘肽还原酶、Cum-OOH存在的条件下,在1分钟内可以催化1微摩尔NADPH转变成NADP+。1 U=1000 mU。
b.对于谷胱甘肽过氧化物酶溶液:1mU/ml=1nmol NADPH/min/ml=(A340/min)/(εμM×L(cm))
即相当于:[检测体系中谷胱甘肽过氧化物酶活力]=(ΔA340/min)/(εμM×L(cm))=[(ΔA340 (sample)-ΔA340 (blank))/min]/ (εμM×L(cm))
[样品中谷胱甘肽过氧化物酶活力]=[检测体系中谷胱甘肽过氧化物酶活力]×[稀释倍数]/[样品中的蛋白浓度]= [(ΔA340/min)/(εμM×L(cm))]×[dil×(V(ml)/Vsample(ml))]/[样品中的蛋白浓度]
注:[检测体系中谷胱甘肽过氧化物酶活力]的单位为mU/ml,[样品中的蛋白浓度]的单位为mg/ml,所以最终[样品中谷胱甘肽过氧化物酶活力]的单位为:U/mg蛋白或mU/mg蛋白;
εμM为摩尔消光系数:NADPH在A340的摩尔消光系数为0.00622μM-1cm-1
L(cm)为测吸光度时的路径长度:100μl样品在一般的96孔中的高度约为0.276cm,如果使用不同的反应孔,请注意修改为溶液在该孔中的高度;
dil为样品的稀释倍数;
V(ml)为反应体系,本反应体系为0.1ml;
c.Vsample(ml)为反应体系中样品的体积,以毫升表示。计算示例:样品的蛋白浓度经测定为1.2mg/ml,用样品稀释液稀释2倍后,取20微升稀释后的样品参考表1进行测定,测定时间设为15分钟。如果0分钟时的A340 (sample)=1.44,A340 (blank)=1.50,15分钟时的A340 (sample)=0.32,A340 (blank)=0.86,则ΔA340 (sample)=1.44-0.32=1.12,ΔA340 (blank)=1.50-0.86=0.64,那么:
[检测体系中谷胱甘肽过氧化物酶活力]= [ (1.12-0.64)/15]/(0.00622×0.276)=18.64mU/ml
[样品中谷胱甘肽过氧化物酶活力]= 18.64mU/ml×(2×0.1/0.02)/(1.2mg/ml)=155mU/mg(蛋白)
相关产品

产品编号 产品名称 包装
S0052 总谷胱甘肽检测试剂盒 100次
S0053 GSH和GSSG检测试剂盒 共100次
S0055 谷胱甘肽还原酶检测试剂盒 100次
S0056 谷胱甘肽过氧化物酶检测试剂盒(NADPH法) 100次
S0057 谷胱甘肽过氧化物酶检测试剂盒(DTNB法) 100次
S0058 总谷胱甘肽过氧化物酶检测试剂盒(NADPH法) 100次
S0059 总谷胱甘肽过氧化物酶检测试剂盒(DTNB法) 100次

总谷胱甘肽过氧化物酶检测试剂盒(S0058)

相关产品请点击如下按钮:
活性氧相关

使用本产品的文献:
1. Xiao-Long W, Chuan-Ping Y, Kai X, Ou-Jv Q.
Selenoprotein W depletion in vitro might indicate that its main function is not as an antioxidative enzyme.
BIOCHEMISTRY-MOSCOW+ . 2010;75(2):201-7. (IF 1.978)

2. Wang J, Sun P, Bao Y, Liu J, An L.
Cytotoxicity of single-walled carbon nanotubes on PC12 cells.
Toxicol In Vitro . 2011 Feb;25(1):242-50. (IF 2.959)

3. Chen J, Xiao S, Deng Y, Du X, Yu Z.
Cloning of a novel glutathione S-transferase 3 (GST3) gene and expressionanalysis in pearl oyster,Pinctada martensii.
FISH SHELLFISH IMMUN . 2011 Dec;31(6):823-30. (IF 3.298)

4. Shi H, Wang Y, Cheng Z, Ye T, Chan Z.
Analysis of natural variation in bermudagrass (Cynodon dactylon) reveals physiological responses underlyingdrought tolerance.
PLoS One . 2012;7(12):e53422. (IF 2.74)

5. Wang J, Sun P, Bao Y, Dou B, Song D, Li Y.
Vitamin E renders protection to PC12 cells against oxidative damage and apoptosis induced bysingle-walled carbon nanotubes.
Toxicol In Vitro . 2012 Feb;26(1):32-41. (IF 2.959)

6. Qian Y, Guan T, Huang M, Cao L, Li Y, Cheng H, Jin H, Yu D.
Neuroprotection by the soy isoflavone, genistein, via inhibition of mitochondria-dependentapoptosis pathways and reactive oxygen induced-NF-κB activation in a cerebral ischemia mouse model.
Neurochem Int . 2012 Jun;60(8):759-67. (IF 3.881)

7. Zhao J, Xu S, Song F, Nian L, Zhou X, Wang S.
2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside protects human umbilical vein endothelial cells against lysophosphatidylcholine-induced apoptosis by upregulating superoxide dismutase and glutathione peroxidase.
IUBMB Life . 2014 Oct;66(10):711-22. (IF 3.244)

8. Li C, Shi L, Chen D, Ren A, Gao T, Zhao M.
Functional analysis of the role of glutathione peroxidase (GPx) in the ROS signaling pathway, hyphal branching and the regulation of ganoderic acid biosynthesis in Ganoderma lucidum.
Fungal Genet Biol . 2015 Sep;82:168-80. (IF 3.071)

9. Yao C, Li G, Qian Y, Cai M, Yin H, Xiao L, Tang W, Guo F, Shi B.
Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia.
Oxid Med Cell Longev . 2016;2016:3406802. (IF 5.076)

10. Sun Y, Xiu C, Liu W, Tao Y, Wang J, Qu YI.
Grape seed proanthocyanidin extract protects the retina against early diabetic injury by activating the Nrf2pathway.
Exp Ther Med . 2016 Apr;11(4):1253-1258. (IF 1.785)

11. Liu Q, Xu C, Ji G, Liu H, Mo Y, Tollerud DJ, Gu A, Zhang Q.
Sublethal effects of zinc oxide nanoparticles on male reproductive cells.
Toxicol In Vitro . 2016 Sep;35:131-8. (IF 2.959)

12. Zhai X, Zhang C, Zhao G, Stoll S, Ren F, Leng X.
Antioxidant capacities of the selenium nanoparticles stabilized by chitosan.
J NANOBIOTECHNOL . 2017 Jan 5;15(1):4. (IF 6.518)

13. Han X, Xue X, Zhao Y, Li Y, Liu W, Zhang J, Fan S.
Rutin-Enriched Extract from Coriandrum sativum L. Ameliorates Ionizing Radiation-InducedHematopoietic Injury.
Int J Mol Sci . 2017 Apr 29;18(5). pii: E942. (IF 4.556)

14. Guo J, Xu B, Han Q, Zhou H, Xia Y, Gong C, Dai X, Li Z, Wu G.
Ferroptosis:A Novel Anti-Tumor Action for Cisplatin.
Cancer Res Treat . 2017 May 10. (IF 3.761)

15. Zhu Z, Ren Z, Fan X, Pan Y, Lv S, Pan C, Lei A, Zeng W.
Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages.
PLoS One . 2017 Jul 10;12(7):e0181110. (IF 2.74)

16. He JH, Yan M, Zuo H, Niu S, Yuan J, Weng SP, He J, Xu X.
High reduced/oxidized glutathione ratio in infectious spleen and kidney necrosis virus-infectedcells contributes to degradation of VP08R multimers.
Vet Microbiol . 2017 Aug;207:19-24. (IF 3.03)

17. Zhu Z, Fan X, Lv Y, Lin Y, Wu, Zeng W.
Glutamine protects rabbit spermatozoa against oxidative stress via glutathione synthesis during cryopreservation.
REPROD FERT DEVELOP. 2017 Oct;29(11):2183-2194. (IF 1.718)

18. Lu MC, Jiao Q, Liu T, Tan SJ, Zhou HS, You QD, Jiang ZY.
Discovery of a head-to-tail cyclic peptide as the Keap1-Nrf2 protein-protein interaction inhibitor with high cell potency.
Eur J Med Chem . 2018 Jan 1;143:1578-1589. (IF 5.572)

19. Fu Q,Li C,Yu L
Gambogic acid inhibits spinal cord injury and inflammation through suppressing the p38 and Akt signaling pathways.
Mol Med Rep . 2018 Jan;17(1):2026-2032. (IF 2.1)

20. Dai J,Gu L,Su Y,Wang Q,Zhao Y,Chen X,Deng H,Li W,Wang G,Li K
Inhibition of curcumin on influenza A virus infection and influenzal pneumonia via oxidative stress, TLR2/4, p38/JNK MAPK and NF-κB pathways.
Int Immunopharmacol . 2018 Jan;54:177-187. (IF 3.943)

21. Qiao H,Ren H,Du H,Zhang M,Xiong X,Lv R
Liraglutide repairs the infarcted heart: The role of the SIRT1/Parkin/mitophagy pathway.
Mol Med Rep . 2018 Mar;17(3):3722-3734. (IF 2.1)

22. Zhao L, Qi L, Li C, Li L, Jin L, Yuan J.
SVCV impairs mitochondria complex Ⅲ resulting in accumulation of hydrogen peroxide.
FISH SHELLFISH IMMUN . 2018 Apr;75:58-65. (IF 3.298)

23. Zhang QL,Wang W,Jiang Y,A-Tuya,Dongmei,Li LL,Lu ZJ,Chang H,Zhang TZ
GRGM-13 comprising 13 plant and animal products, inhibited oxidative stress induced apoptosis in retinal ganglion cells by inhibiting P2RX7/p38 MAPK signaling pathway.
Biomed Pharmacother . 2018 May;101:494-500. (IF 4.545)

24. Chen M,Zheng J,Liu G,Xu E,Wang J,Fuqua BK,Vulpe CD,Anderson GJ,Chen H
Ceruloplasmin and hephaestin jointly protect the exocrine pancreas against oxidative damage by facilitating iron efflux.
Redox Biol . 2018 Jul;17:432-439. (IF 9.986)

25. Zhang Y,Li Q,Fang M,Ma Y,Liu N,Yan X,Zhou J,Li F
The Kidney Injury Induced by Short-Term PM2.5 Exposure and the Prophylactic Treatment of Essential Oils in BALB/c Mice.
Oxid Med Cell Longev . 2018 Jul 29;2018:9098627. (IF 5.076)

26. Xu E,Chen M,Zheng J,Maimaitiming Z,Zhong T,Chen H
Deletion of hephaestin and ceruloplasmin induces a serious systemic iron deficiency and disrupts iron homeostasis.
BIOCHEM BIOPH RES CO . 2018 Sep 10;503(3):1905-1910. (IF 2.985)

27. Deng J,Liu Q,Zhang Q,Zhang C,Liu D,Fan D,Yang H
Comparative study on composition, physicochemical and antioxidant characteristics of different varieties of kiwifruit seed oil in China.
Food Chem . 2018 Oct 30;264:411-418. (IF 6.306)

28. Zhang D,Li Y,Zhang T,Liu J,Jahejo AR,Yang L,Chen P,Ning G,Huo N,Ma H,Yan F,Tian W
Protective effects of zinc and N-acetyl-L-cysteine supplementation against cadmium induced erythrocyte cytotoxicity in Arbor Acres broiler chickens (Gallus gallus domesticus).
ECOTOX ENVIRON SAFE . 2018 Nov 15;163:331-339. (IF 4.872)

29. Ding X,Sun W,Chen J
IL-2 augments the sorafenib-induced apoptosis in liver cancer by promoting mitochondrial fission and activating the JNK/TAZ pathway.
Cancer Cell Int . 2018 Nov 9;18:176. (IF 4.175)

30. Zhang X,Högy P,Wu X,Schmid I,Wang X,Schulze WX,Jiang D,Fangmeier A
Physiological and Proteomic Evidence for the Interactive Effects of Post-Anthesis Heat Stress and Elevated CO2 on Wheat.
Proteomics . 2018 Dec;18(23):e1800262. (IF 3.254)

31. Xu S,Zhao X,Liu F,Cao Y,Wang B,Wang X,Yin M,Wang Q,Feng X
Crucial role of oxidative stress in bactericidal effect of parthenolide against Xanthomonas oryzae pv. oryzae.
Pest Manag Sci . 2018 Dec;74(12):2716-2723. (IF 3.75)

32. Wu Z,Wang Y,Meng X,Wang X,Li Z,Qian S,Wei Y,Shu L,Ding Y,Wang P,Peng Y
Total C-21 steroidal glycosides, isolated from the root tuber of Cynanchum auriculatum Royle ex Wight, attenuate hydrogen peroxide-induced oxidative injury and inflammation in L02 cells.
Int J Mol Med . 2018 Dec;42(6):3157-3170. (IF 3.098)

33. Zhang L,Li S,Wang R,Chen C,Ma W,Cai H
Cytokine augments the sorafenib-induced apoptosis in Huh7 liver cancer cell by inducing mitochondrial fragmentation and activating MAPK-JNK signalling pathway.
Biomed Pharmacother. 2019 Feb;110:213-223. (IF 4.545)

34. Alboghobeish S,Mahdavinia M,Zeidooni L,Samimi A,Oroojan AA,Alizadeh S,Dehghani MA,Ahangarpour A,Khorsandi L
Efficiency of naringin against reproductive toxicity and testicular damages induced by bisphenol A in rats.
Iran J Basic Med Sci. 2019 Mar;22(3):315-523. (IF 2.146)

35. Qin QJ,Cui LQ,Li P,Wang YB,Zhang XZ,Guo ML
Rhynchophylline ameliorates myocardial ischemia/reperfusion injury through the modulation of mitochondrial mechanisms to mediate myocardial apoptosis.
Mol Med Rep. 2019 Apr;19(4):2581-2590. (IF 2.1)

36. Sun X,Li X,Jia H,Loor JJ,Bucktrout R,Xu Q,Wang Y,Shu X,Dong J,Zuo R,Yang L,Liu G,Li X
Effect of heat-shock protein B7 on oxidative stress in adipocytes from preruminant calves.
J Dairy Sci. 2019 Jun;102(6):5673-5685. (IF 3.333)

37. Lu MC,Zhao J,Liu YT,Liu T,Tao MM,You QD,Jiang ZY
CPUY192018, a potent inhibitor of the Keap1-Nrf2 protein-protein interaction, alleviates renal inflammation in mice by restricting oxidative stress and NF-κB activation.
Redox Biol. 2019 Sep;26:101266. (IF 9.986)

38. Qiu WJ,Xu MZ,Zhu XD,Ji YH
MicroRNA-27a alleviates IL-1β-induced inflammatory response and articular cartilage degradation via TLR4/NF-κB signaling pathway in articular chondrocytes.
Int Immunopharmacol. 2019 Nov;76:105839. (IF 3.943)

39. Yu HF,Duan CC,Yang ZQ,Wang YS,Yue ZP,Guo B
HB-EGF Ameliorates Oxidative Stress-Mediated Uterine Decidualization Damage.
Oxid Med Cell Longev. 2019 Dec 2;2019:6170936. (IF 5.076)

40. Hang Yu,Zhidian Wu,Xiaozhi Wang,Chang Gao,Run Liu,Fuxin Kang,Mingming Dai
Protective effects of combined treatment with mild hypothermia and edaravone against cerebral ischemia/reperfusion injury via oxidative stress and Nrf2 pathway regulation
Int J Oncol. 2020 Aug;57(2):500-508.;doi: 10.3892/ijo.2020.5077.

41. Yangyang Chen,Jichun Tang,Yinghong Zhang,Juan Du,Yuanli Wang,Hui Yu,Yanling He
Astaxanthin alleviates gestational diabetes mellitus in mice through suppression of oxidative stress
Naunyn Schmiedebergs Arch Pharmacol. 2020 Dec;393(12):2517-2527.;doi: 10.1007/s00210-020-01861-x.

超氧化物检测试剂盒(S0060)

超氧化物检测试剂盒

产品编号: S0060

产品包装:100次
选择包装
100次

说明书下载

558.00 10
价格: ¥ 558.00

产品简介
使用说明
产品图片
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
S0060 超氧化物检测试剂盒 100次 558.00元

超氧化物检测试剂盒(Superoxide Assay Kit)是一种用于超氧化物快速高灵敏度检测的试剂盒。
本试剂盒利用超氧化物可以还原WST-1产生可溶性有色物质为基础来检测超氧化物。本试剂盒的检测试剂中添加了Catalase(触酶)等,可以清除过氧化氢等过氧化物对WST-1显色的干扰,使测定结果更加准确。并且本试剂盒还提供SOD(超氧化物歧化酶),可以验证本试剂盒测定出的结果是否为超氧化物,以排除检测体系中所用的一些试剂可能产生的干扰。对于本试剂盒,加入SOD后通常可以抑制90%以上的超氧化物。
WST-1是一种类似于MTT的化合物,可以被超氧化物还原生成橙色的formazan。超氧化物产生越多越快,则颜色越深,即相应测定得到的吸光度值越大。
使用WST-1作为检测试剂比用传统的细胞色素c(cytochrome c)检测超氧化物具有多方面的优点。首先,WST-1本身的吸光度非常低(通常OD450为0.02左右,因仪器和酶标板不同而有所不同),而细胞色素c本身的吸光度非常高(通常OD550为0.5左右),因此,使用WST-1与细胞色素c相比,检测灵敏度高很多倍。其次,WST-1的还原产物是稳定的可溶性产物,且对细胞基本无毒性,使WST-1方法更加适合于高通量的筛选。另外,细胞色素c法由于灵敏度的限制,一般不适合用96孔板测定,而WST-1法可以用96孔板测定,使检测更加便捷。用WST-1法或细胞色素c法对超氧化物测定效果比较参考图1。图1中,50万/毫升嗜中性粒细胞在37℃用PMA刺激指定时间,用WST-1或细胞色素c方法分别测定。WST-1法测定OD450,而细胞色素c法测定OD550。另外,WST-1法和luminol法相比,灵敏度相近,但仅须使用普通的酶标仪,无须使用luminol法所需的luminometer,并且检测速度也比luminol法更加快捷。
超氧化物通常指超氧化物阴离子(superoxide anion)O2.-,是一种氧分子的自由基。在呼吸链中,NADPH氧化酶把电子传递给氧分子的时候,就会产生超氧化物阴离子O2.-。超氧化物阴离子O2.-是一种强氧化剂,可以由被刺激的白细胞等产生,从而抵御微生物的感染等。超氧化物阴离子O2.-也可以导致氧化损伤,和许多疾病的发生密切相关。
本试剂盒可以测定100个样品。

超氧化物检测试剂盒(S0060)
图1. WST-1法和细胞色素c法对于超氧化物测定效果的比较。

包装清单:

产品编号 产品名称 包装
S0060-1 超氧化物检测缓冲液 30ml
S0060-2 WST-1溶液 1ml
S0060-3 Catalase溶液 200μl
S0060-4 SOD溶液 20μl
说明书 1份

保存条件:
-20℃保存,一年有效。
注意事项:
WST-1溶液、Catalase溶液和SOD溶液均宜尽量避免反复冻融,可以适当分装。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1. 超氧化物检测工作液的配制:
按照下表比例配制超氧化物检测工作液:

1个检测 5个检测 10个检测 20个检测 50个检测
超氧化物检测缓冲液 200微升 1毫升 2毫升 4毫升 10毫升
WST-1溶液 10微升 50微升 100微升 200微升 500微升
Catalase溶液 2微升 10微升 20微升 40微升 100微升
超氧化物检测工作液 212微升 1.06毫升 2.12毫升 4.24毫升 10.6毫升

2. 悬浮细胞产生超氧化物的检测:
a. 细胞用PBS、Hanks液或生理盐水洗涤一次。
b. 约按5-10万个细胞/毫升的比例,用超氧化物检测工作液悬浮细胞。
c. 在96孔板中,每孔加入200微升用超氧化物检测工作液悬浮的细胞,每孔约1-2万个细胞。
d. 37℃孵育3分钟。
e. 在预定的检测孔中加入预计可以诱导产生超氧化物的刺激物,如PMA等,刺激10-60分钟或其它预定的时间。须至少留一个不加刺激物的孔作为空白对照。选择1-2个加刺激物的孔中加入2微升SOD以验证整个检测体系,加SOD溶液的检测可以不做。试剂盒提供的SOD溶液足够做10个检测。具体检测时的安排参考下表:

细胞 超氧化物检测工作液 刺激物 SOD溶液
空白对照
待测样品
阴性对照

f. 在450nm测定吸光度。如无450nm滤光片,可以使用420-480nm的滤光片。可以使用大于600nm的波长作为参考波长进行双波长测定。
3. 贴壁细胞产生超氧化物的检测:
a. 约按每孔5000-10000个细胞(具体的细胞接种数量须根据细胞的大小和生长速度自行确定)把细胞培养到96孔板中,使第二天或待检测时细胞为80-90%满。
b. 吸去培养液,用PBS、Hanks液或生理盐水洗涤一次。
c. 每孔加入200微升超氧化物检测工作液,37℃孵育3分钟。
d. 在预定的检测孔中加入预计可以诱导产生超氧化物的刺激物,如PMA等,刺激10-60分钟或其它预定的时间。须至少留一个不加刺激物的孔作为空白对照。选择1-2个加刺激物的孔中加入2微升SOD以验证整个检测体系,加SOD溶液的检测可以不做。试剂盒提供的SOD溶液足够做10个检测。具体检测时的安排参考下表:

细胞 超氧化物检测工作液 刺激物 SOD溶液
空白对照
待测样品
阴性对照

e. 在450nm测定吸光度。如无450nm滤光片,可以使用420-480nm的滤光片。可以使用大于600nm的波长作为参考波长进行双波长测定。

超氧化物检测试剂盒(S0060)

相关产品请点击如下按钮:
活性氧相关

使用本产品的文献:
1. Bai F, Ni B, Liu M, Feng Z, Xiong Q, Xiao S, Shao G.
Mycoplasma hyopneumoniae-derived lipid-associated membrane proteins induce apoptosis in porcine alveolar macrophage via increasing nitric oxide production, oxidative stress, and caspase-3 activation.
VET IMMUNOL IMMUNOP . 2013 Sep 15;155(3):155-61. (IF 1.713)

2. Qiu Q, Xiong W, Yang C, Dai X, Dan X, Yang Z, Jiao Y, Xiang Y, Liu G, Hardy P.
Lymphocyte-derived microparticles induce apoptosis of airway epithelial cells through activation of p38 MAPK and production of arachidonic acid.
Apoptosis . 2014 Jul;19(7):1113-27. (IF 4.543)

3. Bai F, Ni B, Liu M, Feng Z, Xiong Q, Shao G.
Mycoplasma hyopneumoniae-derived lipid-associated membrane proteins induce inflammation and apoptosis in porcine peripheral blood mononuclear cells in vitro.
Vet Microbiol . 2015 Jan 30;175(1):58-67. (IF 3.03)

4. Jiang T, Hoekstra J, Heng X, Kang W, Ding J, Liu J, Chen S, Zhang J.
P2X7 receptor is critical in α-synuclein–mediated microglial NADPH oxidase activation.
Neurobiol Aging . 2015 Jul;36(7):2304-18. (IF 4.347)

5. Qiu M, Chen L, Tan G, Ke L, Zhang S, Chen H, Liu J.
A reactive oxygen species activation mechanism contributes to JS-K-induced apoptosis in human bladder cancer cells.
SCI REP-UK . 2015 Oct 13;5:15104. (IF 3.998)

6. Wang D, Ma Y, Yang X, Xu X, Zhao Y, Zhu Z, Wang X, Deng H, Li C, Gao F, Tong J, Yamanaka K, An Y.
Hypermethylation of the Keap1 gene inactivates its function, promotes Nrf2 nuclear accumulation, and is involved in arsenite-induced human keratinocyte transformation.
FREE RADICAL BIO MED . 2015 Dec;89:209-19. (IF 6.17)

7. Shi J, Chen Z, Wang L, Wang B, Xu L, Hou L, Zhang Z.
A tumor-specific cleavable nanosystem of PEG-modified [email protected] hybrid aggregates for radio frequency-controlled release, hyperthermia, photodynamic therapy and X-ray imaging.
Acta Biomater . 2016 Jan 1;29:282-97. (IF 7.242)

8. Xu P, Wang H, Li Z, Yang Z.
Triptolide attenuated injury via inhibiting oxidative stress in Amyloid-Beta25-35-treated differentiated PC12 cells.
Life Sci . 2016 Jan 15;145:19-26. (IF 3.647)

9. Hong Q, Ma ZC, Huang H, Wang YG, Tan HL, Xiao CR, Liang QD, Zhang HT, Gao Y.
Antithrombotic activities of ferulic acid via intracellular cyclic nucleotide signaling.
Eur J Pharmacol . 2016 Apr 15;777:1-8. (IF 3.263)

10. Li W, Cheong YK, Wang H, Ren G, Yang Z.
Neuroprotective Effects of Etidronate and 2,3,3-Trisphosphonate Against Glutamate-Induced Toxicity inPC12 Cells.
Neurochem Res . 2016 Apr;41(4):844-54. (IF 3.038)

11. Ishag HZ, Wu YZ, Liu MJ, Xiong QY, Feng ZX, Yang RS, Shao GQ.
In vitro protective efficacy of Lithium chloride against Mycoplasma hyopneumoniae infection.
Res Vet Sci . 2016 Jun;106:93-6. (IF 1.892)

12. Gu LL, Zhang XY, Xing WM, Xu JD, Lu H.
Andrographolide-induced apoptosis in human renal tubular epithelial cells: Roles of endoplasmic reticulumstress and inflammatory response.
ENVIRON TOXICOL CHEM . 2016 Jul;45:257-64. (IF 3.152)

13. He JL, Zhao M, Xia JJ, Guan J, Liu Y, Wang LQ, Song DX, Qu MY, Zuo M, Wen X, Yu X, Huo R, Pan ZW, Ban T, Zhang Y, Zhu JX, Shou W, Qiao GF, Li BY.
FGF21 ameliorates the neurocontrol of blood pressure in the high fructose-drinking rats.
SCI REP-UK . 2016 Jul 8;6:29582. (IF 3.998)

14. Chen Y, Hu Y, Liu S, Zheng H, Wu X, Huang Z, Li H, Peng B, Long J, Pan B, Huang C, Dong Q.
Whole-body aerosol exposure of cadmium chloride (CdCl2) and tetrabromobisphenol A (TBBPA) induced hepatic changes in CD-1 male mice.
J Hazard Mater . 2016 Nov 15;318:109-16. (IF 9.038)

15. Li SJ, Sun SJ, Gao J, Sun FB.
Wogonin induces Beclin-1/PI3K and reactive oxygen species-mediated autophagy in human pancreaticcancer cells.
Oncol Lett . 2016 Dec;12(6):5059-5067. (IF 2.311)

16. Jia XJ, Li X, Wang F, Liu HQ, Zhang DJ, Chen Y.
Berbamine Exerts Anti-Inflammatory Effects via Inhibition of NF-κB and MAPK SignalingPathways.
CELL PHYSIOL BIOCHEM . 2017;41(6):2307-2318. (IF 5.5)

17. Wang G, Jin W, Qasim AM, Gao A, Peng X, Li W, Feng H, Chu PK.
Antibacterial effects of titanium embedded with silver nanoparticles based on electron-transfer-inducedreactive oxygen species.
Biomaterials . 2017 Jan 30;124:25-34. (IF 10.317)

18. Qiu M, Chen L, Tan G, Ke L, Zhang S, Chen H, Liu J.
JS-K promotes apoptosis by inducing ROS production in human prostate cancer cells.
Oncol Lett . 2017 Mar;13(3):1137-1142. (IF 2.311)

19. Dai F, Li Q, Wang Y, Ge C, Feng C, Xie S, He H, Xu X, Wang C.
Design, Synthesis,and Biological Evaluation of Mitochondria-Targeted Flavone-Naphthalimide-Polyamine Conjugates with Antimetastatic Activity.
J Med Chem . 2017 Mar 9;60(5):2071-2083. (IF 6.205)

20. Wang G, Jin W, Qasim AM, Gao A, Peng X, Li W, Feng H, Chu PK.
Antibacterial effects of titanium embedded with silver nanoparticles based on electron-transfer-induced reactive oxygen species.
Biomaterials . 2017 Apr;124:25-34. (IF 10.317)

21. Li S, Li P, Zhang L, Hu W, Wang M, Liu Y, Tang G, Wang D, Zhou B, Yan J.
The role of reactive oxygen intermediates in the intracellular fate of Leptospira interrogans in the macrophages of different hosts.
PLoS One . 2017 Jun 2;12(6):e0178618. (IF 2.74)

22. Liu H,Zhang Z,Xu M,Xu R,Wang Z,Di G
K6PC-5 Activates SphK1-Nrf2 Signaling to Protect Neuronal Cells from Oxygen Glucose Deprivation/Re-Oxygenation.
CELL PHYSIOL BIOCHEM . 2018;51(4):1908-1920. (IF 5.5)

23. Qiu M, Shi F, Dai F, Song R, Wang S, You Y, Zhao B.
A reactive oxygen species activation mechanism contributes to Sophoridine-induced apoptosis in rat liver BRL-3A cells.
J Ethnopharmacol . 2018 Mar 1;213:376-383. (IF 3.69)

24. Li X, Wang B, Tang L, Zhang Y, Chen L, Gu L, Zhang F, Ouyang J, Zhang X.
GSTA1 Expression Is Correlated With Aldosterone Level in KCNJ5-Mutated Adrenal Aldosterone-Producing Adenoma.
J CLIN ENDOCR METAB . 2018 Mar 1;103(3):813-823. (IF 5.399)

25. Mo J,Yang R,Li F,Zhang X,He B,Zhang Y,Chen P,Shen Z
Scutellarin protects against vascular endothelial dysfunction and prevents atherosclerosis via antioxidation.
Phytomedicine . 2018 Mar 15;42:66-74. (IF 4.268)

26. Wang K,Hu L,Chen JK
RIP3-deficience attenuates potassium oxonate-induced hyperuricemia and kidney injury.
Biomed Pharmacother . 2018 May;101:617-626. (IF 4.545)

27. Li S,Fu L,Tian T,Deng L,Li H,Xia W,Gong Q
Disrupting SOD1 activity inhibits cell growth and enhances lipid accumulation in nasopharyngeal carcinoma.
Cell Commun Signal . 2018 Jun 11;16(1):28. (IF 4.344)

28. Gao LX,Bian C,Wu Y,Nisar MF,Chen S,Li CM,Yu L,Ji P,Huang E,Zhong JL
Label-free electrochemical sensor to investigate the effect of tocopherol on generation of superoxide ions following UV irradiation.
J Biol Eng . 2018 Sep 12;12:17. (IF 3.429)

29. Song Z,Wang H,Wu Y,Gu J,Li S,Han H
Fabrication of Bis-Quaternary Ammonium Salt as an Efficient Bactericidal Weapon Against Escherichia coli and Staphylococcus aureus.
ACS Omega . 2018 Oct 31;3(10):14517-14525. (IF 2.87)

30. Liu H,Feng Y,Xu M,Yang J,Wang Z,Di G
Four-octyl itaconate activates Keap1-Nrf2 signaling to protect neuronal cells from hydrogen peroxide.
Cell Commun Signal . 2018 Nov 15;16(1):81. (IF 4.344)

31. Zuo H,Yuan J,Yang L,Liang Z,Weng S,He J,Xu X
Characterization and immune function of the thioredoxin-interacting protein (TXNIP) from Litopenaeus vannamei.
FISH SHELLFISH IMMUN. 2019 Jan;84:20-27. (IF 3.298)

32. Guan J,Zhao M,He C,Li X,Li Y,Sun J,Wang W,Cui YL,Zhang Q,Li BY,Qiao GF
Anti-Hypertensive Action of Fenofibrate via UCP2 Upregulation Mediated by PPAR Activation in Baroreflex Afferent Pathway.
Neurosci Bull. 2019 Feb;35(1):15-24. (IF 4.326)

33. Zhang L,Zhao P,Yue C,Jin Z,Liu Q,Du X,He Q
Sustained release of bioactive hydrogen by Pd hydride nanoparticles overcomes Alzheimer’s disease.
Biomaterials. 2019 Mar;197:393-404. (IF 10.317)

34. Song Z,Wu Y,Wang H,Han H
Synergistic antibacterial effects of curcumin modified silver nanoparticles through ROS-mediated pathways.
MAT SCI ENG C-MATER. 2019 Jun;99:255-263. (IF 5.88)

35. Zhao Q,Li H,Chang L,Wei C,Yin Y,Bei H,Wang Z,Liang J,Wu Y
Qiliqiangxin Attenuates Oxidative Stress-Induced Mitochondrion-Dependent Apoptosis in Cardiomyocytes via PI3K/AKT/GSK3β Signaling Pathway.
Biol Pharm Bull. 2019 Aug 1;42(8):1310-1321. (IF 1.863)

36. Han M,Hu L,Chen Y
Rutaecarpine may improve neuronal injury, inhibits apoptosis, inflammation and oxidative stress by regulating the expression of ERK1/2 and Nrf2/HO-1 pathway in rats with cerebral ischemia-reperfusion
DRUG DES DEV THER. 2019 Aug 20;13:2923-2931. (IF 3.216)

37. Mo Y,Zhu JL,Jiang A,Zhao J,Ye L,Han B
Compound 13 activates AMPK-Nrf2 signaling to protect neuronal cells from oxygen glucose deprivation-reoxygenation.
AGING-US. 2019 Dec 18;11(24):12032-12042. (IF 4.831)

38. Tianlei Sun,Cheryl H T Kwong,Cheng Gao,Jianwen Wei,Ludan Yue,Jianxiang Zhang,Richard Dequan Ye,Ruibing Wang
Amelioration of ulcerative colitis via inflammatory regulation by macrophage-biomimetic nanomedicine
Theranostics. 2020 Aug 8;10(22):10106-10119.;doi: 10.7150/thno.48448.

39. Jing Wu,Yiping Ni,Qianlei Yang,Jiayuan Mao,Xuerui Zhu,Shasha Tao,Koichi Kato,Jie Zhang,Dapeng Wang,Kenzo Yamanaka,Yan An
Long-term arsenite exposure decreases autophagy by increased release of Nrf2 in transformed human keratinocytes
Sci Total Environ. 2020 Sep 10;734:139425.;doi: 10.1016/j.scitotenv.2020.139425.

40. Chenyang Duan,Li Wang,Jie Zhang,Xinming Xiang,Yue Wu,Zisen Zhang,Qinghui Li,Kunlun Tian,Mingying Xue,Liangming Liu,Tao Li
Mdivi-1 attenuates oxidative stress and exerts vascular protection in ischemic/hypoxic injury by a mechanism independent of Drp1 GTPase activity
Redox Biol. 2020 Oct;37:101706.;doi: 10.1016/j.redox.2020.101706.

41. Fang Luo,Sushan Luo,Wenjing Qian,Lin Zhang,Chen Chen,Meimei Xu,Guangling Wang,Zhongfeng Wang,Jian Wang,Wenyuan Wang
Developmental deficits and early signs of neurodegeneration revealed by PD patient derived dopamine neurons
Stem Cell Res. 2020 Dec;49:102027.;doi: 10.1016/j.scr.2020.102027.