HA抗体(小鼠单抗)(AH158)

HA抗体(小鼠单抗)
HA抗体(小鼠单抗)(AH158) 一键复制产品信息

产品编号: AH158

产品包装:>40次
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>40次

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585.00 565.00 10

价格: ¥ 585.00

促销价: ¥ 585.00

促销价: ¥ 585.00 565.00

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产品编号 产品名称 产品包装 产品价格
AH158 HA抗体(小鼠单抗) >40次 585.00元
来源 用途 抗体识别位点 抗体类型
Mouse WB, IP, IF, IHC, E HA tag IgG1

      WB,Western blot;IP,Immunoprecipitation;IF,Immunofluorescence;IHC,Immunohistochemistry;E,ELISA.
      本HA抗体(HA antibody)为进口分装,用经过适当修饰的人工合成human influenza virushemagglutinin(HA)98-106位多肽YPYDVPDYA作为抗原制备而成的抗HA小鼠单克隆抗体。克隆号为HA-7。
      本HA抗体可以用于识别human influenza virus hemagglutinin(HA),但更为广泛地用于HA tag融合表达蛋白的检测。本抗体可以检测位于融合蛋白N-terminal的HA tag,也可检测位于融合蛋白C-terminal的HA tag。
      DNA重组技术使给目标蛋白带上特定tag成为可能,这些常用的tag包括myc、HA、Flag、His、GST等。带上tag后,通常不会影响目标蛋白的生物活性和细胞内定位。但检测和tag融合表达的目标蛋白时,使用商业化的相应tag抗体就可以完成,大大方便了很多实验检测。
      HA抗体可以用于检测和HA tag融合表达蛋白的表达、细胞内定位,以及纯化、定性或定量检测HAtag融合表达蛋白等。
      配套提供了Western一抗稀释液,可以用于Western检测时的一抗稀释。
      建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC E
1:1000 1:50 1:300 1:300 1:100

      本抗体如果用于常规的Western检测,至少可以检测40次。
包装清单:

产品编号 产品名称 包装
AH158-1 HA抗体(小鼠单抗) 40μl
AH158-2 Western一抗稀释液 40ml
说明书 1份

保存条件:
      HA抗体-20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项: 
      在Western实验后,请注意回收稀释的抗体。回收的抗体在进行Western实验时至少可以重复使用10次。稀释后的抗体,包括已经使用过的稀释抗体,4℃保存。
      回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1.Western检测:
A.按照1:1000用碧云天提供的Western一抗稀释液稀释抗体。
B.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动1-2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
C.回收稀释的一抗,4℃保存以备下次继续使用。
D.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2.免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页: http://www.beyotime.com/support/immunol-staining.htm
3.其它实验操作请自行参考适当的protocol进行。

HA抗体(小鼠单抗)(AH158)
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