GFP抗体(小鼠单抗)(AG281) 一键复制产品信息

产品编号: AG281




471.00 455.00 10

价格: ¥ 471.00

促销价: ¥ 471.00

促销价: ¥ 471.00 455.00

产品编号 产品名称 产品包装 产品价格
AG281 GFP抗体(小鼠单抗) >40次 471.00元
来源 用途 抗体识别位点 抗体类型 GFP分子量
Mouse WB, IP, IF Full length GFP IgG2a ~27kD

      WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence.
      本GFP抗体(小鼠单抗),即mouse monoclonal GFP antibody,为进口分装,用全长的来源于水母的GFP (green
fluorescent protein)作为抗原制备得到的抗GFP小鼠单克隆抗体。克隆号为B-2。
      本GFP抗体可以识别GFP以及GFP的一些突变体例如EGFP(enhanced green fluorescent protein)。

1:200-1000 1:100 1:200


产品编号 产品名称 包装
AG281-1 GFP抗体(小鼠单抗) 40μl
AG281-2 Western一抗稀释液 40ml
说明书 1份


1. Western检测:
A. 按照1:1000用碧云天提供的Western一抗稀释液稀释抗体。如果GFP阳性的细胞低于30%,建议适当提高抗体浓度,例如按照1:500或1:200稀释GFP抗体。
B. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
C. 回收稀释的一抗,4℃保存以备下次继续使用。
D. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
2. 免疫染色:
3. 其它实验操作请自行参考适当的protocol进行。








1. Zhao J, Dong L, Lu B, Wu G, Xu D, Chen J, Li K, Tong X, Dai J, Yao S, Wu M, Guo Y.
Down-regulation of osteopontin suppresses growth and metastasis of hepatocellular carcinoma via induction of apoptosis.
Gastroenterology. 2008 Sep;135(3):956-68. (IF 17.373)

2. Yue X, Zhang Z, Liang X, Gao L, Zhang X, Zhao D, Liu X, Ma H, Guo M, Spear BT, Gong Y, Ma C.
Zinc fingers and homeoboxes 2 inhibits hepatocellular carcinoma cell proliferation and repressesexpression of cyclins a and e.
Gastroenterology. 2012 Jun;142(7):1559-70. (IF 17.373)

3. Wei Y, Chang Y, Zeng H, Liu G, He C, Shi H.
RAV transcription factors are essential for disease resistance against cassava bacterial blight viaactivation of melatonin biosynthesis genes.
J Pineal Res. 2018 Jan;64(1).doi: 10.1111/jpi.12454. (IF 14.528)

4. Wei Y, Chang Y, Zeng H, Liu G, He C, Shi H
RAV transcription factors are essential for disease resistance against cassava bacterial blight via activation of melatonin biosynthesis genes.
J Pineal Res. 2018 Jan;64(1). (IF 14.528)

5. Chen Y, Huang C, Bai C, Du C, Liao J, Dong Q.
In vivo DNA mismatch repair measurement in zebrafish embryos and its use in screening of environmental carcinogens.
J Hazard Mater. 2016 Jan 25;302:296-303. (IF 9.038)

6. Yanling Yan, Shuai Liu, Can Hu, Chaoyi Xie, Linyue Zhao, Shimin Wang, Wenjuan Zhang, Zihang Cheng, Jinghu Gao, Xin Fu, Zhenrong Yang, Xianghong Wang, Jing Zhang, Long Lin, Anbing Shi
RTKN-1/Rhotekin shields endosome-associated F-actin from disassembly to ensure endocytic recycling
J Cell Biol. 2021 May 3;220(5):e202007149.;doi: 10.1083/jcb.202007149. (IF 8.811)

7. Liu Y, Ji X, Nie X, Qu M, Zheng L, Tan Z, Zhao H, Huo L, Liu S, Zhang B, Wang Y.
Arabidopsis AtbHLH112 regulates the expression of genes involved in abiotic stress tolerance by binding to their E-box and GCG-box motifs.
New Phytol. 2015 Aug;207(3):692-709. (IF 8.512)

8. Yunxie Wei, Wen Liu, Wei Hu, Yu Yan, Haitao Shi
The chaperone MeHSP90 recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava
New Phytol. 2020 Apr;226(2):476-491.;doi: 10.1111/nph.16346. (IF 8.512)

9. Qiang L, Wang J, Zhang Y, Ge P, Chai Q, Li B, Shi Y, Zhang L, Gao GF, Liu CH
Mycobacterium tuberculosis Mce2E suppresses the macrophage innate immune response and promotes epithelial cell proliferation.
Cell Mol Immunol. 2019 Apr;16(4):380-391. (IF 8.484)

10. Yu Yan, Peng Wang, Yunxie Wei, Yujing Bai, Yi Lu, Hongqiu Zeng, Guoyin Liu, Russel J Reiter, Chaozu He, Haitao Shi
The dual interplay of RAV5 in activating nitrate reductases and repressing catalase activity to improve disease resistance in cassava
Plant Biotechnol J. 2021 Apr;19(4):785-800.;doi: 10.1111/pbi.13505. (IF 8.154)

11. Yunxie Wei, Binbin Zhu, Wen Liu, Xiao Cheng, Daozhe Lin, Chaozu He, Haitao Shi
Heat shock protein 90 co-chaperone modules fine-tune the antagonistic interaction between salicylic acid and auxin biosynthesis in cassava
Cell Rep. 2021 Feb 2;34(5):108717.;doi: 10.1016/j.celrep.2021.108717. (IF 8.109)

12. Peng Wang, Yu Yan, Yujing Bai, Yabin Dong, Yunxie Wei, Hongqiu Zeng, Haitao Shi
Phosphorylation of RAV1/2 by KIN10 is essential for transcriptional activation of CAT6/7, which underlies oxidative stress response in cassava
Cell Rep. 2021 Dec 14;37(11):110119.;doi: 10.1016/j.celrep.2021.110119. (IF 8.109)

13. Susu Guo, Yuxin Chen, Yueyue Yang, Xiao Zhang, Lifang Ma, Xiangfei Xue, Yongxia Qiao, Jiayi Wang
TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress
Cell Death Dis. 2021 Jan 7;12(1):42.;doi: 10.1038/s41419-020-03299-8. (IF 6.304)

14. Chen Z, Xie X, Huang J, Gong W, Zhu X, Chen Q, Huang J, Huang H.
Connexin43 regulates high glucose-induced expression of fibronectin, ICAM-1 and TGF-β1 via Nrf2/AREpathway in glomerular mesangial cells.
FREE RADICAL BIO MED. 2017 Jan;102:77-86. (IF 6.17)

15. Yang Z, Xiong F, Wang Y, Gong W, Huang J, Chen C, Liu P, Huang H.
TGR5 activation suppressed S1P/S1P2 signaling and resisted high glucose-induced fibrosis inglomerular mesangial cells.
Pharmacol Res. 2016 Sep;111:226-36. (IF 5.893)

16. Zhang W, Yao Y, Chen J, Wang M.
Unconserved C terminal of human cytomegalovirus tegument protein pUL76 elicits nuclear aggresome formation and induces DNA damage in transfected cells.
J Biomed Sci. 2015 Oct 22;22:95. (IF 5.762)

17. He K, He J, Wang S, Yan J.
hSHIP induces S-phase arrest and growth inhibition in cervical cancer HeLa cells.
J Genet Genomics. 2010;37(4):249-55. (IF 5.065)

18. Sun Y, Wang J, Lao H, Yin Z, He W, Weng S, Yu X, Chan S, He J.
Molecular cloning and expression analysis of the ASC gene from mandarin fish and its regulation of NF-kappaB activation.
Dev Comp Immunol. 2008;32(4):391-9. (IF 3.192)

19. Guo M, Huang T, Cui Y, Pan B, Shen A, Sun Y, Yi Y, Wang Y, Xiao G, Sun G.
PrPC interacts with tetraspanin-7 through bovine PrP154-182 containing alpha-helix 1.
BIOCHEM BIOPH RES CO. 2008 Jan 4;365(1):154-7. (IF 2.985)

20. Wang JM, Wang LF, Shi ZL.
Construction of a non-infectious SARS coronavirus replicon for application in drug screening and analysis of viral protein function.
BIOCHEM BIOPH RES CO. 2008 Sep 12;374(1):138-42. (IF 2.985)

21. Peng SW, Zhu LY, Chen M, Zhang M, Li DZ, Fu YC, Chen SR, Wei CJ.
Heterogeneity in mitotic activity and telomere length implies an important role of young islets in the maintenance of islet mass in the adult pancreas.
Endocrinology. 2009 Jul;150(7):3058-66. (IF 3.934)

22. Dong L, Xu H, Liu YB, Lu B, Xu DM, Li BH, Gao J, Wu M, Yao SD, Zhao J, Guo YJ.
M-PEIs nanogels: potential nonviral vector for systemic plasmid delivery to tumor cells.
Cancer Gene Ther. 2009 Jul;16(7):561-6. (IF 4.534)

23. Li J, Wang C, Zhang JH, Cai JM, Cao YP, Sun XJ.
Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer’s disease by reduction of oxidative stress.
Brain Res. 2010;1328:152-61. (IF 2.733)

24. Ma Z, Cao M, Liu Y, He Y, Wang Y, Yang C, Wang W, Du Y, Zhou M, Gao F.
Mitochondrial F1Fo-ATP synthase translocates to cell surface in hepatocytes and has high activityin tumor-like acidic and hypoxic environment.
ACTA BIOCH BIOPH SIN. 2010 Aug;42(8):530-7. (IF 2.836)

25. Zhuang W, Jia Z, Feng H, Chen J, Wang H, Guo Y, Meng C.
The mechanism ofthe G0/G1 cell cycle phase arrest induced by activation of PXR in human cells.
Biomed Pharmacother. 2011 Oct;65(7):467-73. (IF 4.545)

26. Mu W, Guan L, Yan Y, Liu Q, Zhang Y
A novel in vivo inducible expression system in Edwardsiella tarda for potential application inbacterial polyvalence vaccine.
FISH SHELLFISH IMMUN. 2011 Dec;31(6):1097-105. (IF 3.298)

27. Ma Z, Zhang H, Zheng J, Li Y, Yi L, Fan H, Lu C.
Interaction between M-like protein and macrophage thioredoxin facilitates antiphagocytosis forStreptococcus equi ssp. zooepidemicus.
PLoS One. 2012;7(2):e32099. (IF 2.74)

28. Li N, Xu X, Xiao B, Zhu ED, Li BS, Liu Z, Tang B, Zou QM, Liang HP, Mao XH
H. pylori related proinflammatory cytokines contribute to the induction of miR-146a in humangastric epithelial cells.
Mol Biol Rep. 2012 Apr;39(4):4655-61. (IF 1.402)

29. Liu H, Gu D, Cao X, Liu Q, Wang Q, Zhang Y.
Characterization of a new quorum sensing regulator luxT and its roles in the extracellular proteaseproduction, motility, and virulence in fish pathogen Vibrio alginolyticus.
Arch Microbiol. 2012 Jun;194(6):439-52. (IF 1.884)

30. Li H, Gao F, Ma L, Jiang J, Miao J, Jiang M, Fan Y, Wang L, Wu D, Liu B, Wang W, Lui VC, Yuan Z.
Therapeutic potential of in utero mesenchymal stem cell (MSCs) transplantation in rat fetuses withspina bifida aperta.
J Cell Mol Med. 2012 Jul;16(7):1606-17. (IF 4.486)

31. Zhao S, Zhang G, Dai X, Hou Y, Li M, Liang J, Liang C.
Processing and intracellular localization of rice stripe virus Pc2 protein in insect cells.
Virology. 2012 Aug 1;429(2):148-54. (IF 2.819)

32. Pei Z, Lan X, Cheng Z, Qin C, Wang P, He Y, Yen TC, Tian Y, Mghanga FP, Zhang Y.
A multimodality reporter gene for monitoring transplanted stem cells.
Nucl Med Biol. 2012 Aug;39(6):813-20. (IF 2.396)

33. Li W, Gao H, Yin C, Xu X.
Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria.
Microbiology. 2012 Sep;158(Pt 9):2440-9. (IF 1.027)

34. Wang J, Yan DW, Yuan TT, Gao X, Lu YT.
A gain-of-function mutation in IAA8 alters Arabidopsis floral organ development by change of jasmonic acid level.
Plant Mol Biol. 2013 May;82(1-2):71-83. (IF 3.302)

35. Li H, Miao J, Zhao G, Wu D, Liu B, Wei X, Cao S, Gu H, Zhang Y, Wang L, Fan Y, Yuan Z.
Different expression patterns of growth factors in rat fetuses with spina bifida aperta after in utero mesenchymal stromal cell transplantation.
Cytotherapy. 2014 Mar;16(3):319-30. (IF 4.218)

36. Wang J, Su H, Zhang T, Du J, Cui S, Yang F, Jin Q.
Inhibition of Enterovirus 71 replication by 7-hydroxyflavone and diisopropyl-flavon7-yl Phosphate.
PLoS One. 2014 Mar 24;9(3):e92565. (IF 2.74)

37. Zhang J, He Q, Zhang CD, Chen XY, Chen XM, Dong ZQ, Li N, Kuang XX, Cao MY, Lu C, Pan MH.
Inhibition of BmNPV replication in silkworm cells using inducible and regulated artificial microRNA precursors targeting the essential viral gene lef-11.
ANTIVIR RES. 2014 Apr;104:143-52. (IF 4.101)

38. Wang J, Du J, Jin Q.
Class I ADP-ribosylation factors are involved in enterovirus 71 replication.
PLoS One. 2014 Jun 9;9(6):e99768. (IF 2.74)

39. Yang P, Li Z, Fu R, Wu H, Li Z.
Pyruvate kinase M2 facilitates colon cancer cell migration via the modulation of STAT3 signalling.
Cell Signal. 2014 Sep;26(9):1853-62. (IF 3.968)

40. Long D, Lu W, Zhang Y, Guo Q, Xiang Z, Zhao A
New insight into the mechanism underlying fibroin secretion in silkworm, Bombyx mori.
FEBS J. 2015 Jan;282(1):89-101. (IF 4.392)

41. Ji X, Liu G, Liu Y, Nie X, Zheng L, Wang Y.
The regulatory network of ThbZIP1 in response to abscisic acid treatment.
Front Plant Sci. 2015 Feb 10;6:25. (IF 4.402)

42. Du S, Liu C, Zhu Y, Wang Y, Ren D, Wang M, Tan P, Li X, Tian M, Zhang Y, Li J, Zhao F, Li C, Jin N.
Construction and characterization of novel fowlpox virus shuttle vectors.
Virus Res. 2015 Feb 2;197:59-66. (IF 2.934)

43. Mei Z, Zhang D, Hu B, Wang J, Shen X, Xiao W.
FBXO32 Targets c-Myc for Proteasomal Degradation and Inhibits c-Myc Activity.
J Biol Chem. 2015 Jun 26;290(26):16202-14. (IF 4.238)

44. Zhang L, Li Z, Fan Y, Li H, Li Z, Li Y.
Overexpressed GRP78 affects EMT and cell-matrix adhesion via autocrine TGF-β/SmaD/3 signaling.
INT J BIOCHEM CELL B. 2015 Jul;64:202-11. (IF 3.673)

45. Yu J, Qiu H, Liu X, Wang M, Gao Y, Chory J, 4, Tao Y.
Characterization of tub4P287L , a β-tubulin mutant, revealed new aspects of microtubule regulation in shade.
J Integr Plant Biol. 2015 Sep;57(9):757-69. (IF 4.885)

46. Cao S, Wei X, Li H, Miao J, Zhao G, Wu D, Liu B, Zhang Y, Gu H, Wang L, Fan Y, An D, Yuan Z.
Comparative Study on the Differentiation of Mesenchymal Stem Cells Between Fetal and Postnatal RatSpinal Cord Niche.
Cell Transplant. 2016;25(6):1115-30. (IF 3.341)

47. Yan Y, Wang P, He C, Shi H.
MeWRKY20 and its interacting and activating autophagy-related protein 8 (MeATG8) regulateplant disease resistance in cassava.
BIOCHEM BIOPH RES CO. 2017 Dec 9;494(1-2):20-26. (IF 2.985)

48. Shi H, Liu G, Wei Y, Chan Z
The zinc-finger transcription factor ZAT6 is essential for hydrogen peroxide induction of anthocyanin synthesis in Arabidopsis.
Plant Mol Biol. 2018 May;97(1-2):165-176. (IF 3.302)

49. Liu WJ, Yang YT, Huang YM, Zhou DR, Xu DN, Cao N, Jiang DL, Pan JQ, Tian YB
Identification of Goose PKR Gene: Structure, Expression Profiling, and Antiviral Activity Against Newcastle Disease Virus.
J INTERF CYTOK RES. 2018 Aug;38(8):333-340. (IF 2.032)

50. Liu W, Yan Y, Zeng H, Li X, Wei Y, Liu G, He C, Shi H
Functional characterization of WHY-WRKY75 transcriptional module in plant response to cassava bacterial blight.
Tree Physiol. 2018 Oct 1;38(10):1502-1512. (IF 3.655)

51. Wei Y, Liu G, Chang Y, He C, Shi H
Heat shock transcription factor 3 regulates plant immune response through modulation of salicylic acid accumulation and signalling in cassava.
Mol Plant Pathol. 2018 Oct;19(10):2209-2220. (IF 4.326)

52. Ze Yu, Hao Wang, Yilin Fang, Liangliang Lu, Minghao Li, Bingru Yan, Yuzhe Nie, Chunbo Teng
Molecular chaperone HspB2 inhibited pancreatic cancer cell proliferation via activating p53 downstream gene RPRM, BAI1, and TSAP6
J Cell Biochem. 2020 Mar;121(3):2318-2329.;doi: 10.1002/jcb.29455. (IF 4.237)

53. Tao Guo, Lei Zhang, Hong Xiao, Yan Yang, Yawei Shi
Ezrin interacts with L-periaxin by the “head to head and tail to tail” mode and influences the location of L-periaxin in Schwann cell RSC96
BBA-GEN SUBJECTS. 2020 Apr;1864(4):129520.;doi: 10.1016/j.bbagen.2020.129520. (IF 3.422)

54. Hua Cai, Wenxia Yao, Jingxian Huang, Jing Xiao, Wenli Chen, Longbo Hu, Runming Mai, Mengdi Liang, Di Chen, Nan Jiang, Li Zhou, Tao Peng
Apolipoprotein M, identified as a novel hepatitis C virus (HCV) particle associated protein, contributes to HCV assembly and interacts with E2 protein
ANTIVIR RES. 2020 May;177:104756.;doi: 10.1016/j.antiviral.2020.104756. (IF 4.101)

55. Yuming Chen, Shuangqi Fan, Mengpo Zhao, Keke Wu, Erpeng Zhu, Shengming Ma, Wencheng He, Shaofeng Deng, Hailuan Xu, Jingyuan Zhang, Hongxing Ding, Lin Yi, Mingqiu Zhao, Jinding Chen
MG132 Attenuates the Replication of Classical Swine Fever Virus in vitro
Front Microbiol. 2020 Jun 3;11:852.;doi: 10.3389/fmicb.2020.00852. (IF 4.235)

56. Yue Gong, Yanwei Bi, Zhihua Li, Yuzhong Li, Yueting Yao, Qiong Long, Tao Pu, Chen Chen, Tongyun Liu, Shaozhong Dong, Wei Cun
High-efficiency nonhomologous insertion of a foreign gene into the herpes simplex virus genome
J Gen Virol. 2020 Sep;101(9):982-996.;doi: 10.1099/jgv.0.001451. (IF 3.376)

57. Jindai Fan, Mengru Zhang, Chenchen Liu, Mengjiao Zhu, Zilin Zhang, Keke Wu, Zhaoyao Li, Wenhui Li, Shuangqi Fan, Chunmei Ju, Lin Yi, Hongxing Ding, Mingqiu Zhao, Jinding Chen
The Network of Interactions Between Classical Swine Fever Virus Nonstructural Protein p7 and Host Proteins
Front Microbiol. 2020 Nov 30;11:597893.;doi: 10.3389/fmicb.2020.597893. (IF 4.235)

58. Hanchong Zhang, Zihang Cheng, Wenbo Li, Jie Hu, Linyue Zhao, Dan Chen, Jinghu Gao, Juan Chen, Yanling Yan, Long Lin, Anbing Shi
WTS-1/LATS regulates endocytic recycling by restraining F-actin assembly in a synergistic manner
J Cell Sci. 2021 Dec 15;134(24):jcs259085.;doi: 10.1242/jcs.259085. (IF 4.573)