Human BMP-2 重组人骨形态发生蛋白2(人BMP-2)

发表于2025年4月12日由上海金畔生物科技有限公司

产品说明书

FAQ

COA

已发表文献

骨形态发生蛋白 2 (BMP-2)是 BMP亚群的成员，其中BMP2-7属于 TGF-β超家族。它在胚胎背腹模式、器官发生、肢芽形成以及骨形成和再生中起主导作用。成熟的人BMP-2 与小鼠和大鼠 BMP-2具有100% 的aa序列同一性。它与人类 BMP-4 的序列同一性为 85%，与其他 BMP 的序列同一性不到 51%。正常的骨形成是一个经过仔细调节的长期过程，涉及成骨细胞在增殖和最终分化时连续表达生长调节因子。由于成骨细胞基因表达的这种有序序列表明了级联效应，BMP-2 能够启动和维持这种效应。 BMP-2 还促进结肠上皮的维持和修复，抑制神经元多巴胺的合成和释放，诱导髓母细胞瘤细胞凋亡，并且是心肌收缩力所必需的。

产品性质

别名	BDA2, BMP2, BMP-2, BMP-2A, SSFSC
Uniprot No.	P12643
GeneID	650
表达区间及表达系统	E.coli-derived human BMP-2, Gln283-Arg396, with an N-terminal Met.
分子量	Approximately 26.0 kDa.
标签	None
外观	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度	>95 % by SDS-PAGE and HPLC analyses.
生物活性	The ED₅₀ as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/mL, corresponding to a specific activity of > 5.0 × 10³ IU/mg. Fully biologically active when compared to standard.
内毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制剂	Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM sodium citrate, pH 3.5.
重构方法	建议在打开之前进行短暂离心，以将内容物带到底部。在无菌蒸馏水中复溶至 0.1-1.0 mg/mL 的浓度。储备溶液等分分装并储存在 ≤ -20°C。在适当的缓冲溶液中进行进一步稀释。不要在PBS 或细胞培养基中复溶。

运输与保存方法

冰袋运输。冻干粉-20 ℃保存一年有效期。复溶后，蛋白溶液–20 °C保存3个月，2-8°C 保存一周。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

HB221031

产品性质

别名	BDA2, BMP2, BMP-2, BMP-2A, SSFSC
Uniprot No.	P12643
GeneID	650
表达区间及表达系统	E.coli-derived human BMP-2, Gln283-Arg396, with an N-terminal Met.
分子量	Approximately 26.0 kDa.
标签	None
外观	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度	>95 % by SDS-PAGE and HPLC analyses.
生物活性	The ED₅₀ as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/mL, corresponding to a specific activity of > 5.0 × 10³ IU/mg. Fully biologically active when compared to standard.
内毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制剂	Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM sodium citrate, pH 3.5.
重构方法	建议在打开之前进行短暂离心，以将内容物带到底部。在无菌蒸馏水中复溶至 0.1-1.0 mg/mL 的浓度。储备溶液等分分装并储存在 ≤ -20°C。在适当的缓冲溶液中进行进一步稀释。不要在PBS 或细胞培养基中复溶。

运输与保存方法

冰袋运输。冻干粉-20 ℃保存一年有效期。复溶后，蛋白溶液–20 °C保存3个月，2-8°C 保存一周。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

HB221031

暂无内容

SB 4 BMP信号激动剂SB4|CAS 100874-08-6

发表于2025年4月11日由上海金畔生物科技有限公司

SB 4 BMP信号激动剂SB4|CAS 100874-08-6

产品说明书

FAQ

COA

已发表文献

SB 4 (SB4, BMP signaling agonist sb4, Benepali, Brenzys, Etanercept biosimilar)，苯并恶唑化合物，是具有细胞渗透性的BMP4 signaling选择性激动剂，EC₅₀值为74 nM，通过促进SMAD1/5/9的磷酸化激活BMP信号。BMP信号在发育、组织稳态和疾病过程中具有重要作用。典型的BMP信号由SMAD-1/5/8/9转导，它们与SMAD-4形成转录复合物。SMAD异聚复合物易位至细胞核可募集组织特异性转录因子，从而驱动直接BMP靶基因的表达。

产品性质

英文别名 (English Synonym)	SB 4, SB4, BMP signaling agonist sb4, Benepali, Brenzys, Etanercept biosimilar
靶点 (Target)	BMP4 signal
通路 (Pathway)	TGF-β/Smad–TGF-β Receptor
CAS号 (CAS NO.)	100874-08-6
分子式 (Formula)	C₁₄H₁₀BrNOS
分子量 (Molecular Weight)	320.20
外观 (Appearance)	粉末
纯度 (Purity)	≥98%
溶解性 (Solubility)	溶于DMSO
结构式 (Structure)

运输和保存方法

冰袋运输。粉末直接保存于-20℃，有效期3年。建议分装后-20℃干燥保存，避免反复冻融。

注意事项

1. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

2. 粉末溶解前请先短暂离心，以保证产品全在管底。

3. 请勿吸入、吞咽或者直接接触皮肤和眼睛。

4. 本产品仅用于科研用途，禁止用于人身上。

使用浓度

【具体使用浓度请参考相关文献，并根据自身实验条件（如实验目的，细胞种类，培养特性等）进行摸索和优化。】

使用方法（数据来自于公开发表的文献，仅供参考）

细胞实验（体外实验）

SB 4 (0.05-1 μM)以剂量依赖性在血清饥饿条件下诱导PRECs细胞中SMAD-1/5/9磷酸化。SB 4 (1 μM)可提高rhBMP4各浓度下的信号传递效率，SB 4在0.4 ng/mL rhBMP4时使BRE-luc表达增加2倍。^[¹^]

参考文献

1.Bradford STJ, et al. High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds.J Biol Chem. 2019 Mar 1;294(9):3125-3136.

HB221117

产品性质

英文别名 (English Synonym)	SB 4, SB4, BMP signaling agonist sb4, Benepali, Brenzys, Etanercept biosimilar
靶点 (Target)	BMP4 signal
通路 (Pathway)	TGF-β/Smad–TGF-β Receptor
CAS号 (CAS NO.)	100874-08-6
分子式 (Formula)	C₁₄H₁₀BrNOS
分子量 (Molecular Weight)	320.20
外观 (Appearance)	粉末
纯度 (Purity)	≥98%
溶解性 (Solubility)	溶于DMSO
结构式 (Structure)

运输和保存方法

冰袋运输。粉末直接保存于-20℃，有效期3年。建议分装后-20℃干燥保存，避免反复冻融。

注意事项

1. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

2. 粉末溶解前请先短暂离心，以保证产品全在管底。

3. 请勿吸入、吞咽或者直接接触皮肤和眼睛。

4. 本产品仅用于科研用途，禁止用于人身上。

使用浓度

【具体使用浓度请参考相关文献，并根据自身实验条件（如实验目的，细胞种类，培养特性等）进行摸索和优化。】

使用方法（数据来自于公开发表的文献，仅供参考）

细胞实验（体外实验）

参考文献

1.Bradford STJ, et al. High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds.J Biol Chem. 2019 Mar 1;294(9):3125-3136.

HB221117

暂无内容

Human BMP-7 重组人骨形态发生蛋白7(人BMP-7)

发表于2025年1月23日由上海金畔生物科技有限公司

Human BMP-7 重组人骨形态发生蛋白7(人BMP-7)

产品说明书

FAQ

COA

已发表文献

产品信息

产品名称	产品编号	规格
Recombinant Human BMP-7 Protein	92054ES10	10 μg
Recombinant Human BMP-7 Protein	92054ES50	50 μg

产品简介

BMP-7 is a member of the TGF-β superfamily which is supposed to be one of the most potent anabolic factors of chondrocytes. The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7.

性能参数

Synonyms	BMP7, BMP-7, bone morphogenetic protein 7, Eptotermin alfa, OP-1,
Uniprot No.	P18075
Source	Human BMP-7 Protein is expressed from HEK293 without tag. It contains Met316-His431.
Molecular Weight	The protein has a predicted 13.1 kDa.
Purity	> 95% as determined by SDS-PAGE.
Biological Activity	Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 20-40 ng/ml.
Endotoxin	< 1 0EU /mg of the protein by the LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution containing 0.085% TFA, 30% ACN,5% mannitol.
Reconstitution	Centrifuge the tube before opening. It is recommended to redissolve in 4 mM HCl.

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3-6 months, –85~–65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

Please operate with lab coats and disposable gloves,for your safety.
This product is for research use only.

Ver. CN20231221

产品信息

产品名称	产品编号	规格
Recombinant Human BMP-7 Protein	92054ES10	10 μg
Recombinant Human BMP-7 Protein	92054ES50	50 μg

产品简介

性能参数

Synonyms	BMP7, BMP-7, bone morphogenetic protein 7, Eptotermin alfa, OP-1,
Uniprot No.	P18075
Source	Human BMP-7 Protein is expressed from HEK293 without tag. It contains Met316-His431.
Molecular Weight	The protein has a predicted 13.1 kDa.
Purity	> 95% as determined by SDS-PAGE.
Biological Activity	Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 20-40 ng/ml.
Endotoxin	< 1 0EU /mg of the protein by the LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution containing 0.085% TFA, 30% ACN,5% mannitol.
Reconstitution	Centrifuge the tube before opening. It is recommended to redissolve in 4 mM HCl.

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3-6 months, –85~–65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

Please operate with lab coats and disposable gloves,for your safety.
This product is for research use only.

Ver. CN20231221

暂无内容

Human BMP-4 重组人骨形态发生蛋白4(人BMP-4)

发表于2025年1月4日由上海金畔生物科技有限公司

Human BMP-4 重组人骨形态发生蛋白4(人BMP-4)

产品说明书

FAQ

COA

已发表文献

Bone morphogenetic protein-4 (BMP-4) is one of nine structurally related BMPs belonging to the transforming growth factor-beta (TGF-beta) superfamily of secreted proteins. The human BMP-4 shares 98 % sequence indentity with mouse BMP-4. Mature BMP-4 is a dimer that binds to a multimeric transmembrane receptor with serine/threonine kinase activity. BMP-4, a stimulator of chondrogenesis, both in vitro and in vivo, is a potential therapeutic agent for cartilage regeneration.

Product Properties

Synonyms	BMP2B, BMP-2B, BMP2B1, BMP2BMCOPS6, BMP4,DVR4, MCOPS6, OFC11, ZYME
Accession	P12644
Source	E.coli-derived human BMP-4 protein, Ser293-Arg408, E399D.
Molecular Weight	The recombinant Human BMP4 predicts a molecular mass of 13.25 kDa.
Tag	None
Purity	> 95% by SDS-PAGE
Biological Activity	Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED₅₀ for this effect is typically 0.08-0.4ug/mL.
Endotoxin	< 1.0 EU per 1μg of the protein by the LAL method.
Formulation	Lyophilized from sterile 30% Acetonitrile, 1/1000 TFA. Normally 5% – 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

Cautions

1.Avoid repeated freeze-thaw cycles.

2.For your safety and health, please wear lab coats and disposable gloves for operation.

3.For research use only.

Ver. CN20230810

Product Properties

Synonyms	BMP2B, BMP-2B, BMP2B1, BMP2BMCOPS6, BMP4,DVR4, MCOPS6, OFC11, ZYME
Accession	P12644
Source	E.coli-derived human BMP-4 protein, Ser293-Arg408, E399D.
Molecular Weight	The recombinant Human BMP4 predicts a molecular mass of 13.25 kDa.
Tag	None
Purity	> 95% by SDS-PAGE
Biological Activity	Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED₅₀ for this effect is typically 0.08-0.4ug/mL.
Endotoxin	< 1.0 EU per 1μg of the protein by the LAL method.
Formulation	Lyophilized from sterile 30% Acetonitrile, 1/1000 TFA. Normally 5% – 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

Cautions

1.Avoid repeated freeze-thaw cycles.

2.For your safety and health, please wear lab coats and disposable gloves for operation.

3.For research use only.

Ver. CN20230810

暂无内容

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标签归档：bmp

Human BMP-2 重组人骨形态发生蛋白2(人BMP-2)

产品说明书

FAQ

COA

已发表文献

SB 4 BMP信号激动剂SB4|CAS 100874-08-6

产品说明书

FAQ

COA

已发表文献

Human BMP-7 重组人骨形态发生蛋白7(人BMP-7)

产品说明书

FAQ

COA

已发表文献

Human BMP-4 重组人骨形态发生蛋白4(人BMP-4)

产品说明书

FAQ

COA

已发表文献