重组人ACE2/ACEH(His标签) 血管紧张素I转换酶

重组人ACE2/ACEH(His标签) 血管紧张素I转换酶

产品说明书

FAQ

COA

已发表文献

 

Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE. ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues. ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo, functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters. Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury, heart disease, liver and lung fibrosis, inflammatory lung disease, and cardiopulmonary disease . Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail. The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters. The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity.

 

产品性质

别名

ACE-2; ACEH; ACE2

Uniprot No.

Q9BYF1.1

表达区间及表达系统

Recombinant Human ACE2/ACEH Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gln18-Ser740.

分子量

Approximately 84.7 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Tris-Bis PAGE result.

纯度

> 95% as determined by SDS-PAGE and HPLC.

活性

SPR Data: SARS-COV-2 Spike RBD captured on Protein A chip, can bind Human ACE2, His Tag with an affinity constant of 11.9nM as determined in a SPR assay (Biacore T200).

内毒素

1.0 EU per 1μg of the protein by the LAL method.

制剂

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

复溶

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输-20-80存,一年有效期

复溶后,20 -80°C,在未开封状态下保存 3-6 个月复溶后,2-8°C 保存 2-7 天。

建议第一次使用时分装存,避免反复冻融。

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途!

 

产品数据
重组人ACE2/ACEH(His标签) 血管紧张素I转换酶
重组人ACE2/ACEH(His标签) 血管紧张素I转换酶

 

HB220803

重组人ACE2/ACEH(His标签) 血管紧张素I转换酶

暂无内容

重组人ACE2/ACEH(His标签) 血管紧张素I转换酶

暂无内容

 

Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE. ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues. ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo, functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters. Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury, heart disease, liver and lung fibrosis, inflammatory lung disease, and cardiopulmonary disease . Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail. The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters. The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity.

 

产品性质

别名

ACE-2; ACEH; ACE2

Uniprot No.

Q9BYF1.1

表达区间及表达系统

Recombinant Human ACE2/ACEH Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gln18-Ser740.

分子量

Approximately 84.7 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Tris-Bis PAGE result.

纯度

> 95% as determined by SDS-PAGE and HPLC.

活性

SPR Data: SARS-COV-2 Spike RBD captured on Protein A chip, can bind Human ACE2, His Tag with an affinity constant of 11.9nM as determined in a SPR assay (Biacore T200).

内毒素

1.0 EU per 1μg of the protein by the LAL method.

制剂

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

复溶

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输-20-80存,一年有效期

复溶后,20 -80°C,在未开封状态下保存 3-6 个月复溶后,2-8°C 保存 2-7 天。

建议第一次使用时分装存,避免反复冻融。

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途!

 

产品数据
重组人ACE2/ACEH(His标签) 血管紧张素I转换酶
重组人ACE2/ACEH(His标签) 血管紧张素I转换酶

 

HB220803

重组人ACE2/ACEH(His标签) 血管紧张素I转换酶

暂无内容

重组人ACE2/ACEH(His标签) 血管紧张素I转换酶

暂无内容