Clontech逆转录病毒载体
pRetroX-IRES-ZsGreen1
| 型号 |
载体名称 |
出品公司 |
载体用途 |
| VNC0591 |
pRetroX-IRES-ZsGreen1 |
Clontech |
逆转录病毒载体 |
Description
pRetroX-IRES-ZsGreen1 is a bicistronic, fl uorescent, retroviral vector that allows both a gene
of interest and the ZsGreen1 gene to be translated from a single bicistronic mRNA. pRetroXIRES-ZsGreen1 is designed for effi cient delivery and selection （by fl ow cytometry or other
methods） of stably transduced mammalian cells expressing the ZsGreen1 fl uorescent protein
and the protein of interest. This vector can be used to obtain stable cell lines without timeconsuming drug and clonal selection. ZsGreen1 is derived from the reef coral Zoanthus sp.
green fl uorescent protein （ZsGreen） and is easily detected with standard FITC fi lter sets （1）.
Bicistronic expression from this vector is facilitated by the encephalomyocarditis virus （EMCV）
internal ribosome entry site （IRES）. This IRES facilitates cap-independent translation from an
internal start site at the IRES/ZsGreen1 junction （2）. ZsGreen1 is a human codon optimized
ZsGreen variant that encodes the brightest commercially available green fl uorescent protein
（1）. This retroviral vector is derived from the pMIN series of vectors （3, 4）. These optimized
vectors have the ability to produce high viral titers, express genes at high levels, and, due
to the absence of retroviral coding sequences, exhibit improved safety profi les. The multiple
cloning site （MCS） in pRetroX-IRES-ZsGreen1 is between the 5’ MMLV LTR and the IRES
sequence. Genes cloned into the MCS are expressed as a bicistronic message transcribed
from the 5’ LTR.
pRetroX-IRES-ZsGreen1 contains all of the necessary viral RNA processing elements; these
include the 5’ and 3’ LTRs, the packaging signal （ψ）, and the tRNA primer-binding site. For
safety reasons, however, the vector lacks the structural genes （gag, pol, and env） necessary for
retroviral particle formation and replication. pRetroX-IRES-ZsGreen1 contains a ColE1 origin
of replication, and an E. coli Ampr
gene for propagation and selection in bacteria.
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