标签归档：载体

pAAV-LacZ载体说明书

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pAAV-LacZ

型号 载体名称 出品公司 载体用途
VXS0411 pAAV-LacZ Stratagene 腺相关病毒系统

Related Products

1. VPK-402: AAV Helper Free Packaging System 

2. AAV-100: 293AAV Cell Line 

3. VPK-140: ViraBind™ AAV Purification Kit 

4. VPK-141: ViraBind™ AAV Purification Mega Kit 

5. VPK-145: QuickTiter™ AAV Quantitation Kit 

6. AAV-200: ViraDuctin™ AAV Transduction Kit 

Safety Considerations

Remember that you will be working with samples containing infectious virus. Follow the 

recommended NIH guidelines for all materials containing BSL-2 organisms. The AAV Helper-Free 

system is designed to minimize the chance of generating wild type AAV, but precautions should still 

be taken to avoid direct contact with viral supernatants. 

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pIκB-EGFP载体说明书

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 pIκB-EGFP

型号 载体名称 出品公司 载体用途
VXC0435 pIκB-EGFP Clontech 信号通路报告载体

Description:

The pIκB-EGFP Vector encodes the IκB-EGFP Signaling Probe, which is a fusion of enhanced green

fluorescent protein （EGFP） and IκB. IκB is an inhibitor of NFκB, a transcription factor involved in the

immune response and inflammatory diseases. When the NFκB pathway is inactive, IκB and NFκB

exist as an inactive complex in the cytosol. Upon stimulation, IκB is degraded. In cells transfected

with pIκB-EGFP, degradation of the IκB-EGFP fusion protein is observed as a decrease in EGFP

fluorescence （1）. The IκB-EGFP Signaling Probe is constitutively expressed and resides in the

cytosol.

EGFP is a red-shifted, human codon-optimized variant of GFP （2–6） that has been engineered for

brighter fluorescence and higher expression in mammalian cells. Its excitation maximum is 488 nm

and emission maximum is 509 nm. For more information on the properties of EGFP, please refer to

the BD Living Colors™ User Manual （PT2040-1） included with the vector.

Expression of the IκB-EGFP is driven by the human CMV immediate-early promoter. The SV40 poly-A

sequence directs proper processing of the 3′ end of the fusion construct. The vector backbone also

contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen. A

neomycin resistance cassette （Neor

） allows kanamycin selection in E. coli and neomycin （G418）

selection in eukaryotic cells. This cassette consists of the SV40 early promoter, the neomycin/

kanamycin resistance gene of Tn5, and poly-A signals from the Herpes simplex virus thymidine

kinase （HSV TK） gene. The vector backbone also provides a pUC origin of replication for

propagation in E. coli and an f1 origin for single-stranded DNA production.

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Pichiapink（strain 1-wildtype）载体说明书

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Pichiapink（strain 1-wildtype）

型号 载体名称 出品公司 载体用途
VBI0309 Pichiapink（strain 1-wildtype） Invitrogen 配套毕赤酵母

The PichiaPink™ Secretion Signal Kit, included in the PichiaPink™ Secretion 

Optimization Kit, is also available separay from Invitrogen （Cat. no. A11155）. 

The PichiaPink™ Secretion Signal Kit consists of eight secretion signal sequences 

supplied as phosphorylated duplexes in 40 pmol lyophilized aliquots. The 

secretion signals and their sources are listed below. Upon receipt, store the 

secretion signal duplexes at –20°C.

pEF1/myc-His C载体说明书

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型号 载体名称 出品公司 载体用途 价格
VPI0028 pEF1/myc-His C Invitrogen 哺乳动物表达载体 800

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：EF-1α
Vector Type：pEF
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 

氨苄青霉素（Ampicillin）

载体描述: 

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit （hEF-1α） promoter for high-level expression across a broad range of species and cell types （Goldman et al., 1996; Mizushima and Nagata, 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc （c-myc） epitope and a polyhistidine metal-binding peptide.
  3. Neomycin resistance gene for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g., COS7）.

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

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pSRE-Luc载体说明书

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 pSRE-Luc

型号 载体名称 出品公司 载体用途
VXS0449 pSRE-Luc Stratagene 信号通路报告载体

Instruction Manual

Catalog #219073 （AP-1 cis-Reporting System） #219075 （CRE cis-Reporting System） 

#219077 （NF-κB cis-Reporting System） #219079 （SRE cis-Reporting System） 

#219081 （SRF cis-Reporting System） #219083 （p53 cis-Reporting System） 

#219092 （ISRE cis-Reporting System） #219093 （GAS cis-Reporting System） 

#219094 （NFAT cis-Reporting System） #219095 （TARE cis-Reporting System） 

#240111 （C/EBP cis-Reporting System） #240113 （DR1 cis-Reporting System） 

#240115 （DR3 cis-Reporting System） #240119 （DR5 cis-Reporting System） 

#240129 （Egr-1 cis-Reporting System） #240131 （LILRE cis-Reporting System） 

#240133 （GRE cis-Reporting System） #240135 （DR4 cis-Reporting System） 

#240049 （pAP-1-hrGFP Plasmid） #240050 （pCRE-hrGFP Plasmid） 

#240051 （pNF-κB-hrGFP Plasmid） #240053 （pNFAT-hrGFP Plasmid） 

#219087 （pLuc-MCS Plasmid） 

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pVSV-G载体

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 pVSV–G载体

Cat.No 描述 出品公司 载体用途
VMC0386 pVSV–G Clontech 慢病毒包装载体
载体描述: 

pVSV-G expresses the G glycoprotein of the vesicular stomatitis virus （VSV-G） under the control of the CMV immediate-early promoter. VSV-G is used in pseudotyping of Moloney Murine Leukenia Virus （MMLV）-based retroviral vectors by mediating viral entry. VSV-G interacts with phospholipid components of the target cell membrane and fosters the fusion of viral and cellular membranes. VSV-G does not require a cell surface receptor and can serve as a surrogate viral envelope protein. pVSV-G Vector includes IVS, a synthetic intron known to enhance the stability of the mRNA, the Col E1 origin of replication and bacterial ampicillin rsistance （Ampr） gene for propagation and antibiotic selection in bacteria.

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pSilencer 2.1-U6 hygro载体说明书

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pSilencer 2.1-U6 hygro

载体描述: 

This Ambion® vector is for the expression of siRNA. It has an antibiotic resistance gene （hygromycin） to enable selection of transfected cells and features the human RNA polymerase III promoter （U6）. Sufficient reagents are provided for 20 reactions.

• Select transfected cells to enrich the population of cells expressing your siRNA 
• Eliminate the need to synthesize RNA oligonucleotides for RNAi experiments
• Supplied linearized and ready for ligation

Compensate for Low Transfection Efficiencies and Perform Long-Term Studies
The use of mammalian siRNA expression vectors with antibiotic selectable markers conveys many benefits. Selectable markers can help compensate for poor plasmid transfection efficiencies seen with some cell lines. In these cases, only a fraction of the transfected cells express the siRNA, and reduction in target gene expression with even a potent siRNA can be difficult to detect. Use of a selectable marker and transient antibiotic selection permits.

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pEF1/myc-His A载体说明书

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型号 载体名称 出品公司 载体用途 价格
VPI0026 pEF1/myc-His A Invitrogen 哺乳动物表达载体 800

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：EF-1α
Vector Type：pEF
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 

氨苄青霉素（Ampicillin）

载体描述: 

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit （hEF-1α） promoter for high-level expression across a broad range of species and cell types （Goldman et al., 1996; Mizushima and Nagata, 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc （c-myc） epitope and a polyhistidine metal-binding peptide.
  3. Neomycin resistance gene for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g., COS7）.

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

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pVAX1载体说明书

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pVAX1

型号 载体名称 出品公司 载体用途
VPI0038 pVAX1 Invitrogen 哺乳动物表达载体
产品参数: 

Mammalian Selection：-
Sequencing Primer：T7 Fwd
Sequencing Primer Sequence：5’d[TAATACGACTCACTATAGGG]3′

载体抗性: 
卡那霉素（Kanamycin）
载体描述: 

pVAX1是在载体pcDNA3.1的基础上改建而成的一种新型真核表达载体，大小为3.0 kb。该质粒用卡那霉素抗性筛选基因替代pcDNA3.1中的氨卞霉素抗性筛选基因，zui大程度地减少抗性筛选基因和人类基因组发生重组的可能性。pVAX1含有多个克隆酶切位点允许同时克隆多个大片段的目的基因，强启动子pCMV和BGH poly A信号可以在哺乳动物细胞中高水平表达重组蛋白。pVAX1又是一种非融合载体，要求插入序列中时必须包含一段KozaK的翻译起始序列和一个起始密码子（ATG）以引导正确的翻译，典型的KozaK共有序列为“ANNATGG”，其-3位的A和+4位的G对正确插入甚为关键，在插入序列中还需包括一个终止密码子以正确终止基因表达。

pVAX1是由美国食品和药品管理委员会（FDA）推荐的*可以应用于人体实验的载体质粒。迄今为止，关于pVAX1的研究已涉及动物和临床试验。

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