重组PE标记人MSLN/间皮素蛋白 Recombinant PE-Labeled Human MSLN/Mesothelin Protein,His-Avi Tag

重组PE标记人MSLN/间皮素蛋白 Recombinant PE-Labeled Human MSLN/Mesothelin Protein,His-Avi Tag

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR

表达区间及表达系统(Source)

PE-Labeled Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu296-Gly580.[Accession | Q13421-2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.2 kDa.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

 

储存条件

The product should be stored at -85~-65℃ for 6 months from date of receipt.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组PE标记人MSLN/间皮素蛋白 Recombinant PE-Labeled Human MSLN/Mesothelin Protein,His-Avi Tag

暂无内容

重组PE标记人MSLN/间皮素蛋白 Recombinant PE-Labeled Human MSLN/Mesothelin Protein,His-Avi Tag

暂无内容

 

性能参数

分子别名(Synonyms)

MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR

表达区间及表达系统(Source)

PE-Labeled Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu296-Gly580.[Accession | Q13421-2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.2 kDa.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

 

储存条件

The product should be stored at -85~-65℃ for 6 months from date of receipt.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组PE标记人MSLN/间皮素蛋白 Recombinant PE-Labeled Human MSLN/Mesothelin Protein,His-Avi Tag

暂无内容

重组PE标记人MSLN/间皮素蛋白 Recombinant PE-Labeled Human MSLN/Mesothelin Protein,His-Avi Tag

暂无内容

重组人MSLN/间皮素蛋白 Recombinant Human MSLN/Mesothelin Protein,His-Avi Tag

重组人MSLN/间皮素蛋白 Recombinant Human MSLN/Mesothelin Protein,His-Avi Tag

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

Mesothelin;MPFSMRP;CAK1;MSLN

表达区间及表达系统(Source)

Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu296-Gly580.[Accession | Q13421-2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Human MSLN, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名(Synonyms)

Mesothelin;MPFSMRP;CAK1;MSLN

表达区间及表达系统(Source)

Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu296-Gly580.[Accession | Q13421-2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Human MSLN, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

异槲皮苷Isoquercetin(NSC39471异槲皮素) 槲皮素-3-葡萄糖苷|CAS 482-35-9

异槲皮苷Isoquercetin(NSC39471异槲皮素) 槲皮素-3-葡萄糖苷|CAS 482-35-9

产品说明书

FAQ

COA

已发表文献

 

Isoquercetin (Quercetin 3-glucoside, Hirsutrin, 3-Glucosylquercetin, NSC39471),又称异槲皮素、异槲皮苷、罗布麻甲素或槲皮素-3-葡萄糖苷是从鬼针草中分离出来类黄酮化合物,也可人工合成,具有抗氧化、降血压和抗炎活性Quercetin-3-glucosideWnt/β-catenin的抑制剂,作用于核易位β-catening的下游。Isoquercetin具有高生物利用度和低毒性,用作辅助药物或药物有效成分,开发前景广阔。

 

产品性质

英文别名 (English Synonym)

Isoquercetin, Quercetin 3-glucoside, Hirsutrin, 3-Glucosylquercetin, NSC39471

中文名称 (Chinese Name)

异槲皮素,异槲皮苷,槲皮素-3-葡萄糖苷,罗布麻甲素

通路 (Pathway)

NF-κB

CAS (CAS NO.)

482-35-9

分子式 (Formula)

C21H20O12

分子量 (Molecular Weight)

464.38

外观 (Appearance)

粉末

纯度 (Purity)

≥98%

溶解性 (Solubility)

DMSO

结构式 (Structure)

异槲皮苷Isoquercetin(NSC39471异槲皮素) 槲皮素-3-葡萄糖苷|CAS 482-35-9

 

运输和保存方法

冰袋运输。粉末直接保存于-20℃,有效期3年。建议分装后-20℃干燥保存,避免反复冻融。

 

注意事项

1. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

2. 粉末溶解前请先短暂离心,以保证产品全在管底。

3. 请勿吸入吞咽或者直接接触皮肤和眼睛

4. 本产品仅用于科研用途,禁止用于人身上。

 

使用浓度

【具体使用浓度请参考相关文献,并根据自身实验条件(如实验目的,细胞种类,培养特性等)进行摸索和优化。

 

使用方法(数据来自于公开发表的文献,仅供参考)

细胞实验(体外实验)

Isoquercetin (5-20 μM)显著降低乙醇诱导的细胞毒性,保护肝细胞免受乙醇刺激的肝损伤。Isoquercetin (10 μM)预处理HepG2细胞显著抑制乙醇诱导的iNOS蛋白表达。[1]

动物实验(体实验)

STZ诱导的糖尿病怀孕小鼠模型中,口服Isoquercetin (100 mg/kg)显著降低了糖尿病小鼠胚胎的神经管缺陷率和细胞凋亡,减轻了内质网应激,降低了p65的水平。[2]

 

参考文献

  1. LaLone CA, et al. Effects of a glucocorticoid receptor agonist, Dexamethasone, on fathead minnow reproduction, growth, and development. Environ Toxicol Chem. 2012 Mar;31(3):611-22.
  2. Tan C, et al. Modulation of nuclear factor-κB signaling and reduction of neural tube defects by quercetin-3-glucoside in embryos of diabetic mice. Am J Obstet Gynecol. 2018 Aug;219(2):197.e1-197.e8.

 

HB221114

异槲皮苷Isoquercetin(NSC39471异槲皮素) 槲皮素-3-葡萄糖苷|CAS 482-35-9

暂无内容

异槲皮苷Isoquercetin(NSC39471异槲皮素) 槲皮素-3-葡萄糖苷|CAS 482-35-9

暂无内容

 

Isoquercetin (Quercetin 3-glucoside, Hirsutrin, 3-Glucosylquercetin, NSC39471),又称异槲皮素、异槲皮苷、罗布麻甲素或槲皮素-3-葡萄糖苷是从鬼针草中分离出来类黄酮化合物,也可人工合成,具有抗氧化、降血压和抗炎活性Quercetin-3-glucosideWnt/β-catenin的抑制剂,作用于核易位β-catening的下游。Isoquercetin具有高生物利用度和低毒性,用作辅助药物或药物有效成分,开发前景广阔。

 

产品性质

英文别名 (English Synonym)

Isoquercetin, Quercetin 3-glucoside, Hirsutrin, 3-Glucosylquercetin, NSC39471

中文名称 (Chinese Name)

异槲皮素,异槲皮苷,槲皮素-3-葡萄糖苷,罗布麻甲素

通路 (Pathway)

NF-κB

CAS (CAS NO.)

482-35-9

分子式 (Formula)

C21H20O12

分子量 (Molecular Weight)

464.38

外观 (Appearance)

粉末

纯度 (Purity)

≥98%

溶解性 (Solubility)

DMSO

结构式 (Structure)

异槲皮苷Isoquercetin(NSC39471异槲皮素) 槲皮素-3-葡萄糖苷|CAS 482-35-9

 

运输和保存方法

冰袋运输。粉末直接保存于-20℃,有效期3年。建议分装后-20℃干燥保存,避免反复冻融。

 

注意事项

1. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

2. 粉末溶解前请先短暂离心,以保证产品全在管底。

3. 请勿吸入吞咽或者直接接触皮肤和眼睛

4. 本产品仅用于科研用途,禁止用于人身上。

 

使用浓度

【具体使用浓度请参考相关文献,并根据自身实验条件(如实验目的,细胞种类,培养特性等)进行摸索和优化。

 

使用方法(数据来自于公开发表的文献,仅供参考)

细胞实验(体外实验)

Isoquercetin (5-20 μM)显著降低乙醇诱导的细胞毒性,保护肝细胞免受乙醇刺激的肝损伤。Isoquercetin (10 μM)预处理HepG2细胞显著抑制乙醇诱导的iNOS蛋白表达。[1]

动物实验(体实验)

STZ诱导的糖尿病怀孕小鼠模型中,口服Isoquercetin (100 mg/kg)显著降低了糖尿病小鼠胚胎的神经管缺陷率和细胞凋亡,减轻了内质网应激,降低了p65的水平。[2]

 

参考文献

  1. LaLone CA, et al. Effects of a glucocorticoid receptor agonist, Dexamethasone, on fathead minnow reproduction, growth, and development. Environ Toxicol Chem. 2012 Mar;31(3):611-22.
  2. Tan C, et al. Modulation of nuclear factor-κB signaling and reduction of neural tube defects by quercetin-3-glucoside in embryos of diabetic mice. Am J Obstet Gynecol. 2018 Aug;219(2):197.e1-197.e8.

 

HB221114

异槲皮苷Isoquercetin(NSC39471异槲皮素) 槲皮素-3-葡萄糖苷|CAS 482-35-9

暂无内容

异槲皮苷Isoquercetin(NSC39471异槲皮素) 槲皮素-3-葡萄糖苷|CAS 482-35-9

暂无内容