第五代耐热逆转录酶(无甘油版)|Hifair® V Reverse Transcriptase

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第五代耐热逆转录酶(无甘油版)|Hifair® V Reverse Transcriptase

产品说明书

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产品简介

 

Hifair® V Reverse Transcriptase是在Hieff® M-MLV (H-) Reverse Transcriptase基础上通过基因工程技术得到的全新逆转录酶，与Hieff® M-MLV (H-) Reverse Transcriptase相比，其热稳定性大幅度提高，可耐受高达60℃的反应温度，适合具有复杂二级结构的RNA模板的逆转录。同时，该酶增强了与模板的亲和力，适合少量模板以及低拷贝基因的逆转录。Hifair® V Reverse Transcriptase合成全长cDNA的能力也有了提升，可扩增长达10 kb的cDNA。

 

产品信息

 

货号

11301ES06 / 11301ES12 / 11301ES62/ 11301ES75 /11301ES96

规格

3 KU / 12 KU / 120 KU / 300 KU / 3000 KU

单位定义

以Poly(A) .Oligo(dT)为模板-引物，在37℃，10 min内，将1 nmol的dTTP掺入为酸不溶性物质所需要的酶量定义为1个活性单位（U）。

 

组分信息

 

组分名称

11301ES06

11301ES12

11301ES62

11301ES75

11301ES96

Hifair® V Reverse Transcriptase (600 U/μL)

5 μL

20 μL

200 μL

500 μL

5 mL

 

储存条件

 

2~8℃保存，有效期6个月。

 

使用说明

 

第一链cDNA合成操作步骤

1. RNA变性（此步为可选步骤，RNA变性有助于打开二级结构，可在很大程度上提高第一链cDNA的产量。）

组分

使用量

RNase free ddH2O

to 13 μL

Oligo (dT)18 (50 μM)

or Random Primers (50 μM)

or Gene Specific Primers (2 μM)

1 μL

or 1 μL

or 1 μL

模板RNA

Total RNA: 1 ng -5 μg或mRNA: 1-500 ng

65℃加热5 min，迅速置于冰上冷却2 min。简短离心收集反应液后，加入下表中的逆转录反应液，并轻轻吹打混匀。

 

2. 逆转录反应体系配制（20 μL体系）

组分

使用量

上一步的反应液

13 μL

5×Hifair® V Buffer

4 μL

dNTP Mix (10 mM)

1 μL

Hifair® V Reverse Transcriptase (600 U/μL)

200 U

RNase inhibitor (40 U/µL)

1 μL

RNase free ddH2O

To 20 μL

3. 逆转录程序设置

温度

时间

25℃*

5 min

42℃**

15-30 min

85℃***

5 min

*当使用Random Primers时，需25℃，孵育5 min；若使用Oligo (dT)18或Gene Specific Primers，此步可省略。

**逆转录温度：推荐使用42℃。对于高GC含量模板或者复杂二级结构的模板，可将逆转录温度提高至50-55℃。

***85℃加热5 min，目的是使逆转录酶失活。

逆转录产物可立即用于后续PCR或qPCR反应，也可-20℃短期保存，若需长期保存，建议分装后，于-80℃保存，避免反复冻融。

该逆转录酶也适用于一步法RT-qPCR，推荐每25 μL反应体系，添加10-20 U逆转录酶，也可根据实际情况逐步增加逆转录酶用量。

 

注意事项

 

1. 请保持实验区域洁净；操作时需穿戴干净的手套、口罩；实验所用耗材均需保证RNase free，以防止RNase污染。

2. 所有操作均应在冰上进行，防止RNA降解。

3. 为保证高效率逆转录，建议使用高质量的RNA样本。

4. 本产品仅用作科研用途。

5. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

 

Ver.CN20231116

Q：这款产品有什么特点?

A：M-MLV (H-) Reverse Transcriptase 是通过对野生型 M-MLV 逆转录酶基因改造，在大肠杆菌中表达纯化而成。. 无 RNaseH 活性，具有合成能力高，热稳定性好和半衰期长的特点。

1. Liu C X, Li X, Nan F, et al. Structure and degradation of circular RNAs regulate PKR activation in innate immunity[J]. Cell, 2019, 177(4): 865-880. e21.（IF31.398）

2. Fan H, Hong B, Luo Y, et al. The effect of whey protein on viral infection and replication of SARS-CoV-2 and pangolin coronavirus in vitro[J]. Signal transduction and targeted therapy, 2020, 5(1): 1-3.(IF13.493)

3. Zhou L, Hou B, Wang D, et al. Engineering Polymeric Prodrug Nanoplatform for Vaccinatio Immunotherapy of Cancer[J]. Nano Letters, 2020.（IF12.279）

4. Wang J., et al., The mycobacterial phosphatase PtpA regulates the expression of host genes and promotes cell proliferation[J]. Nat Commun. 2017 Aug 15;8(1):244.（IF 12.353）

5. Tao L, Yi Y, Chen Y, et al. RIP1 kinase activity promotes steatohepatitis through mediating cell death and inflammation in macrophages[J]. bioRxiv, 2020.( IF10.717)

6. Ma D, Zhao Y, Huang L, et al. A novel hydrogel-based treatment for complete transection spinal cord injury repair is driven by microglia/macrophages repopulation[J]. Biomaterials, 2020, 237: 119830.(IF10.317)

7. Lin Z, Xia S, Liang Y, et al. LXR activation potentiates sorafenib sensitivity in HCC by activating microRNA-378a transcription[J]. Theranostics, 2020, 10(19): 8834. (IF8.579) 11120ES

8. Liu D, Nie W, Li D, et al. 3D printed PCL/SrHA scaffold for enhanced bone regeneration[J]. Chemical Engineering Journal, 2019, 362: 269-279.（IF8.355）

9. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide PtrCLE20 inhibits vascular cambium activity in Populus[J]. Plant Biotechnology Journal, 2020, 18(1): 195-206.(IF8.154)

10. Chen L, Lam J C W, Tang L, et al. Probiotic modulation of lipid metabolism disorders caused by perfluorobutanesulfonate pollution in zebrafish[J]. Environmental Science & Technology, 2020.(IF7.864)

11. Zhao P, Zhang J, Wu A, et al. Biomimetic codelivery overcomes osimertinib-resistant NSCLC and brain metastasis via macrophage-mediated innate immunity[J]. Journal of Controlled Release, 2020. ( IF7.727)

12. Xu X, Gao J, Dai W, et al. Gene activation by a CRISPR-assisted trans enhancer[J]. eLife, 2019, 8: e45973.（IF7.551）

13. Zhao Y, Wang H P, Yu C, et al. Integration of physiological and metabolomic profiles to elucidate the regulatory mechanisms underlying the stimulatory effect of melatonin on astaxanthin and lipids coproduction in Haematococcus pluvialis under inductive stress conditions[J]. Bioresource Technology, 2020, 319: 124150.(IF7.539)

14. Li X, Zhang X, Zhao Y, et al. Cross-talk between gama-aminobutyric acid and calcium ion regulates lipid biosynthesis in Monoraphidium sp. QLY-1 in response to combined treatment of fulvic acid and salinity stress[J]. Bioresource Technology, 2020, 315: 123833.(IF7.539)

15. Nie, W., et al., Three-dimensional porous scaffold by self-assembly of reduced graphene oxide and nano-hydroxyapatite composites for bone tissue engineering[J]. Carbon, 2017.116, 325-337.（IF 7.082）

16. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide Ptr CLE 20 inhibits vascular cambium activity in Populus[J]. Plant biotechnology journal, 2019.（IF6.84）

17. Peng L, Wang Y, Yang B, et al. Polychlorinated biphenyl quinone regulates MLKL phosphorylation that stimulates exosome biogenesis and secretion via a short negative feedback loop[J]. Environmental Pollution, 2020: 115606.(IF6.793)

18. Li A, Liu Q, Li Q, et al. Berberine reduces pyruvate-driven hepatic glucose production by limiting mitochondrial import of pyruvate through mitochondrial pyruvate carrier 1[J]. EBioMedicine, 2018, 34: 243-255. （IF6.68）

19. Lou M D, Li J, Cheng Y, et al. CREB mediates glucagon action to upregulate hepatic MPC1: inhibitory effect of ginsenoside Rb1 on hepatic gluconeogenesis[J]. British journal of pharmacology, 2019.（IF6.583）

20. Fan L, Ye H, Wan Y, et al. Adaptor protein APPL1 coordinates HDAC3 to modulate brown adipose tissue thermogenesis in mice[J]. Metabolism, 2019, 100: 153955.（IF6.513）

1. Liu C X, Li X, Nan F, et al. Structure and degradation of circular RNAs regulate PKR activation in innate immunity[J]. Cell, 2019, 177(4): 865-880. e21.（IF31.398）

2. Fan H, Hong B, Luo Y, et al. The effect of whey protein on viral infection and replication of SARS-CoV-2 and pangolin coronavirus in vitro[J]. Signal transduction and targeted therapy, 2020, 5(1): 1-3.(IF13.493)

3. Zhou L, Hou B, Wang D, et al. Engineering Polymeric Prodrug Nanoplatform for Vaccinatio Immunotherapy of Cancer[J]. Nano Letters, 2020.（IF12.279）

4. Wang J., et al., The mycobacterial phosphatase PtpA regulates the expression of host genes and promotes cell proliferation[J]. Nat Commun. 2017 Aug 15;8(1):244.（IF 12.353）

5. Tao L, Yi Y, Chen Y, et al. RIP1 kinase activity promotes steatohepatitis through mediating cell death and inflammation in macrophages[J]. bioRxiv, 2020.( IF10.717)

6. Ma D, Zhao Y, Huang L, et al. A novel hydrogel-based treatment for complete transection spinal cord injury repair is driven by microglia/macrophages repopulation[J]. Biomaterials, 2020, 237: 119830.(IF10.317)

7. Lin Z, Xia S, Liang Y, et al. LXR activation potentiates sorafenib sensitivity in HCC by activating microRNA-378a transcription[J]. Theranostics, 2020, 10(19): 8834. (IF8.579) 11120ES

8. Liu D, Nie W, Li D, et al. 3D printed PCL/SrHA scaffold for enhanced bone regeneration[J]. Chemical Engineering Journal, 2019, 362: 269-279.（IF8.355）

9. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide PtrCLE20 inhibits vascular cambium activity in Populus[J]. Plant Biotechnology Journal, 2020, 18(1): 195-206.(IF8.154)

10. Chen L, Lam J C W, Tang L, et al. Probiotic modulation of lipid metabolism disorders caused by perfluorobutanesulfonate pollution in zebrafish[J]. Environmental Science & Technology, 2020.(IF7.864)

11. Zhao P, Zhang J, Wu A, et al. Biomimetic codelivery overcomes osimertinib-resistant NSCLC and brain metastasis via macrophage-mediated innate immunity[J]. Journal of Controlled Release, 2020. ( IF7.727)

12. Xu X, Gao J, Dai W, et al. Gene activation by a CRISPR-assisted trans enhancer[J]. eLife, 2019, 8: e45973.（IF7.551）

13. Zhao Y, Wang H P, Yu C, et al. Integration of physiological and metabolomic profiles to elucidate the regulatory mechanisms underlying the stimulatory effect of melatonin on astaxanthin and lipids coproduction in Haematococcus pluvialis under inductive stress conditions[J]. Bioresource Technology, 2020, 319: 124150.(IF7.539)

14. Li X, Zhang X, Zhao Y, et al. Cross-talk between gama-aminobutyric acid and calcium ion regulates lipid biosynthesis in Monoraphidium sp. QLY-1 in response to combined treatment of fulvic acid and salinity stress[J]. Bioresource Technology, 2020, 315: 123833.(IF7.539)

15. Nie, W., et al., Three-dimensional porous scaffold by self-assembly of reduced graphene oxide and nano-hydroxyapatite composites for bone tissue engineering[J]. Carbon, 2017.116, 325-337.（IF 7.082）

16. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide Ptr CLE 20 inhibits vascular cambium activity in Populus[J]. Plant biotechnology journal, 2019.（IF6.84）

17. Peng L, Wang Y, Yang B, et al. Polychlorinated biphenyl quinone regulates MLKL phosphorylation that stimulates exosome biogenesis and secretion via a short negative feedback loop[J]. Environmental Pollution, 2020: 115606.(IF6.793)

18. Li A, Liu Q, Li Q, et al. Berberine reduces pyruvate-driven hepatic glucose production by limiting mitochondrial import of pyruvate through mitochondrial pyruvate carrier 1[J]. EBioMedicine, 2018, 34: 243-255. （IF6.68）

19. Lou M D, Li J, Cheng Y, et al. CREB mediates glucagon action to upregulate hepatic MPC1: inhibitory effect of ginsenoside Rb1 on hepatic gluconeogenesis[J]. British journal of pharmacology, 2019.（IF6.583）

20. Fan L, Ye H, Wan Y, et al. Adaptor protein APPL1 coordinates HDAC3 to modulate brown adipose tissue thermogenesis in mice[J]. Metabolism, 2019, 100: 153955.（IF6.513）

产品简介

 

Hifair® V Reverse Transcriptase是在Hieff® M-MLV (H-) Reverse Transcriptase基础上通过基因工程技术得到的全新逆转录酶，与Hieff® M-MLV (H-) Reverse Transcriptase相比，其热稳定性大幅度提高，可耐受高达60℃的反应温度，适合具有复杂二级结构的RNA模板的逆转录。同时，该酶增强了与模板的亲和力，适合少量模板以及低拷贝基因的逆转录。Hifair® V Reverse Transcriptase合成全长cDNA的能力也有了提升，可扩增长达10 kb的cDNA。

 

产品信息

 

货号

11301ES06 / 11301ES12 / 11301ES62/ 11301ES75 /11301ES96

规格

3 KU / 12 KU / 120 KU / 300 KU / 3000 KU

单位定义

以Poly(A) .Oligo(dT)为模板-引物，在37℃，10 min内，将1 nmol的dTTP掺入为酸不溶性物质所需要的酶量定义为1个活性单位（U）。

 

组分信息

 

组分名称

11301ES06

11301ES12

11301ES62

11301ES75

11301ES96

Hifair® V Reverse Transcriptase (600 U/μL)

5 μL

20 μL

200 μL

500 μL

5 mL

 

储存条件

 

2~8℃保存，有效期6个月。

 

使用说明

 

第一链cDNA合成操作步骤

1. RNA变性（此步为可选步骤，RNA变性有助于打开二级结构，可在很大程度上提高第一链cDNA的产量。）

组分

使用量

RNase free ddH2O

to 13 μL

Oligo (dT)18 (50 μM)

or Random Primers (50 μM)

or Gene Specific Primers (2 μM)

1 μL

or 1 μL

or 1 μL

模板RNA

Total RNA: 1 ng -5 μg或mRNA: 1-500 ng

65℃加热5 min，迅速置于冰上冷却2 min。简短离心收集反应液后，加入下表中的逆转录反应液，并轻轻吹打混匀。

 

2. 逆转录反应体系配制（20 μL体系）

组分

使用量

上一步的反应液

13 μL

5×Hifair® V Buffer

4 μL

dNTP Mix (10 mM)

1 μL

Hifair® V Reverse Transcriptase (600 U/μL)

200 U

RNase inhibitor (40 U/µL)

1 μL

RNase free ddH2O

To 20 μL

3. 逆转录程序设置

温度

时间

25℃*

5 min

42℃**

15-30 min

85℃***

5 min

*当使用Random Primers时，需25℃，孵育5 min；若使用Oligo (dT)18或Gene Specific Primers，此步可省略。

**逆转录温度：推荐使用42℃。对于高GC含量模板或者复杂二级结构的模板，可将逆转录温度提高至50-55℃。

***85℃加热5 min，目的是使逆转录酶失活。

逆转录产物可立即用于后续PCR或qPCR反应，也可-20℃短期保存，若需长期保存，建议分装后，于-80℃保存，避免反复冻融。

该逆转录酶也适用于一步法RT-qPCR，推荐每25 μL反应体系，添加10-20 U逆转录酶，也可根据实际情况逐步增加逆转录酶用量。

 

注意事项

 

1. 请保持实验区域洁净；操作时需穿戴干净的手套、口罩；实验所用耗材均需保证RNase free，以防止RNase污染。

2. 所有操作均应在冰上进行，防止RNA降解。

3. 为保证高效率逆转录，建议使用高质量的RNA样本。

4. 本产品仅用作科研用途。

5. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

 

Ver.CN20231116

Q：这款产品有什么特点?

A：M-MLV (H-) Reverse Transcriptase 是通过对野生型 M-MLV 逆转录酶基因改造，在大肠杆菌中表达纯化而成。. 无 RNaseH 活性，具有合成能力高，热稳定性好和半衰期长的特点。

1. Liu C X, Li X, Nan F, et al. Structure and degradation of circular RNAs regulate PKR activation in innate immunity[J]. Cell, 2019, 177(4): 865-880. e21.（IF31.398）

2. Fan H, Hong B, Luo Y, et al. The effect of whey protein on viral infection and replication of SARS-CoV-2 and pangolin coronavirus in vitro[J]. Signal transduction and targeted therapy, 2020, 5(1): 1-3.(IF13.493)

3. Zhou L, Hou B, Wang D, et al. Engineering Polymeric Prodrug Nanoplatform for Vaccinatio Immunotherapy of Cancer[J]. Nano Letters, 2020.（IF12.279）

4. Wang J., et al., The mycobacterial phosphatase PtpA regulates the expression of host genes and promotes cell proliferation[J]. Nat Commun. 2017 Aug 15;8(1):244.（IF 12.353）

5. Tao L, Yi Y, Chen Y, et al. RIP1 kinase activity promotes steatohepatitis through mediating cell death and inflammation in macrophages[J]. bioRxiv, 2020.( IF10.717)

6. Ma D, Zhao Y, Huang L, et al. A novel hydrogel-based treatment for complete transection spinal cord injury repair is driven by microglia/macrophages repopulation[J]. Biomaterials, 2020, 237: 119830.(IF10.317)

7. Lin Z, Xia S, Liang Y, et al. LXR activation potentiates sorafenib sensitivity in HCC by activating microRNA-378a transcription[J]. Theranostics, 2020, 10(19): 8834. (IF8.579) 11120ES

8. Liu D, Nie W, Li D, et al. 3D printed PCL/SrHA scaffold for enhanced bone regeneration[J]. Chemical Engineering Journal, 2019, 362: 269-279.（IF8.355）

9. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide PtrCLE20 inhibits vascular cambium activity in Populus[J]. Plant Biotechnology Journal, 2020, 18(1): 195-206.(IF8.154)

10. Chen L, Lam J C W, Tang L, et al. Probiotic modulation of lipid metabolism disorders caused by perfluorobutanesulfonate pollution in zebrafish[J]. Environmental Science & Technology, 2020.(IF7.864)

11. Zhao P, Zhang J, Wu A, et al. Biomimetic codelivery overcomes osimertinib-resistant NSCLC and brain metastasis via macrophage-mediated innate immunity[J]. Journal of Controlled Release, 2020. ( IF7.727)

12. Xu X, Gao J, Dai W, et al. Gene activation by a CRISPR-assisted trans enhancer[J]. eLife, 2019, 8: e45973.（IF7.551）

13. Zhao Y, Wang H P, Yu C, et al. Integration of physiological and metabolomic profiles to elucidate the regulatory mechanisms underlying the stimulatory effect of melatonin on astaxanthin and lipids coproduction in Haematococcus pluvialis under inductive stress conditions[J]. Bioresource Technology, 2020, 319: 124150.(IF7.539)

14. Li X, Zhang X, Zhao Y, et al. Cross-talk between gama-aminobutyric acid and calcium ion regulates lipid biosynthesis in Monoraphidium sp. QLY-1 in response to combined treatment of fulvic acid and salinity stress[J]. Bioresource Technology, 2020, 315: 123833.(IF7.539)

15. Nie, W., et al., Three-dimensional porous scaffold by self-assembly of reduced graphene oxide and nano-hydroxyapatite composites for bone tissue engineering[J]. Carbon, 2017.116, 325-337.（IF 7.082）

16. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide Ptr CLE 20 inhibits vascular cambium activity in Populus[J]. Plant biotechnology journal, 2019.（IF6.84）

17. Peng L, Wang Y, Yang B, et al. Polychlorinated biphenyl quinone regulates MLKL phosphorylation that stimulates exosome biogenesis and secretion via a short negative feedback loop[J]. Environmental Pollution, 2020: 115606.(IF6.793)

18. Li A, Liu Q, Li Q, et al. Berberine reduces pyruvate-driven hepatic glucose production by limiting mitochondrial import of pyruvate through mitochondrial pyruvate carrier 1[J]. EBioMedicine, 2018, 34: 243-255. （IF6.68）

19. Lou M D, Li J, Cheng Y, et al. CREB mediates glucagon action to upregulate hepatic MPC1: inhibitory effect of ginsenoside Rb1 on hepatic gluconeogenesis[J]. British journal of pharmacology, 2019.（IF6.583）

20. Fan L, Ye H, Wan Y, et al. Adaptor protein APPL1 coordinates HDAC3 to modulate brown adipose tissue thermogenesis in mice[J]. Metabolism, 2019, 100: 153955.（IF6.513）

1. Liu C X, Li X, Nan F, et al. Structure and degradation of circular RNAs regulate PKR activation in innate immunity[J]. Cell, 2019, 177(4): 865-880. e21.（IF31.398）

2. Fan H, Hong B, Luo Y, et al. The effect of whey protein on viral infection and replication of SARS-CoV-2 and pangolin coronavirus in vitro[J]. Signal transduction and targeted therapy, 2020, 5(1): 1-3.(IF13.493)

3. Zhou L, Hou B, Wang D, et al. Engineering Polymeric Prodrug Nanoplatform for Vaccinatio Immunotherapy of Cancer[J]. Nano Letters, 2020.（IF12.279）

4. Wang J., et al., The mycobacterial phosphatase PtpA regulates the expression of host genes and promotes cell proliferation[J]. Nat Commun. 2017 Aug 15;8(1):244.（IF 12.353）

5. Tao L, Yi Y, Chen Y, et al. RIP1 kinase activity promotes steatohepatitis through mediating cell death and inflammation in macrophages[J]. bioRxiv, 2020.( IF10.717)

6. Ma D, Zhao Y, Huang L, et al. A novel hydrogel-based treatment for complete transection spinal cord injury repair is driven by microglia/macrophages repopulation[J]. Biomaterials, 2020, 237: 119830.(IF10.317)

7. Lin Z, Xia S, Liang Y, et al. LXR activation potentiates sorafenib sensitivity in HCC by activating microRNA-378a transcription[J]. Theranostics, 2020, 10(19): 8834. (IF8.579) 11120ES

8. Liu D, Nie W, Li D, et al. 3D printed PCL/SrHA scaffold for enhanced bone regeneration[J]. Chemical Engineering Journal, 2019, 362: 269-279.（IF8.355）

9. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide PtrCLE20 inhibits vascular cambium activity in Populus[J]. Plant Biotechnology Journal, 2020, 18(1): 195-206.(IF8.154)

10. Chen L, Lam J C W, Tang L, et al. Probiotic modulation of lipid metabolism disorders caused by perfluorobutanesulfonate pollution in zebrafish[J]. Environmental Science & Technology, 2020.(IF7.864)

11. Zhao P, Zhang J, Wu A, et al. Biomimetic codelivery overcomes osimertinib-resistant NSCLC and brain metastasis via macrophage-mediated innate immunity[J]. Journal of Controlled Release, 2020. ( IF7.727)

12. Xu X, Gao J, Dai W, et al. Gene activation by a CRISPR-assisted trans enhancer[J]. eLife, 2019, 8: e45973.（IF7.551）

13. Zhao Y, Wang H P, Yu C, et al. Integration of physiological and metabolomic profiles to elucidate the regulatory mechanisms underlying the stimulatory effect of melatonin on astaxanthin and lipids coproduction in Haematococcus pluvialis under inductive stress conditions[J]. Bioresource Technology, 2020, 319: 124150.(IF7.539)

14. Li X, Zhang X, Zhao Y, et al. Cross-talk between gama-aminobutyric acid and calcium ion regulates lipid biosynthesis in Monoraphidium sp. QLY-1 in response to combined treatment of fulvic acid and salinity stress[J]. Bioresource Technology, 2020, 315: 123833.(IF7.539)

15. Nie, W., et al., Three-dimensional porous scaffold by self-assembly of reduced graphene oxide and nano-hydroxyapatite composites for bone tissue engineering[J]. Carbon, 2017.116, 325-337.（IF 7.082）

16. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide Ptr CLE 20 inhibits vascular cambium activity in Populus[J]. Plant biotechnology journal, 2019.（IF6.84）

17. Peng L, Wang Y, Yang B, et al. Polychlorinated biphenyl quinone regulates MLKL phosphorylation that stimulates exosome biogenesis and secretion via a short negative feedback loop[J]. Environmental Pollution, 2020: 115606.(IF6.793)

18. Li A, Liu Q, Li Q, et al. Berberine reduces pyruvate-driven hepatic glucose production by limiting mitochondrial import of pyruvate through mitochondrial pyruvate carrier 1[J]. EBioMedicine, 2018, 34: 243-255. （IF6.68）

19. Lou M D, Li J, Cheng Y, et al. CREB mediates glucagon action to upregulate hepatic MPC1: inhibitory effect of ginsenoside Rb1 on hepatic gluconeogenesis[J]. British journal of pharmacology, 2019.（IF6.583）

20. Fan L, Ye H, Wan Y, et al. Adaptor protein APPL1 coordinates HDAC3 to modulate brown adipose tissue thermogenesis in mice[J]. Metabolism, 2019, 100: 153955.（IF6.513）