Anti-Pro-SP-C – Rabbit, n-terminal (WRAB-9337)使用说明
产品信息
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品牌
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Seven Hills Bioreagents
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货号
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WRAB-9337
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规格
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100 µL
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宿主
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兔
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反应种属
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人、鼠
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克隆类型
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多克隆
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偶 联 物
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无
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缓冲物
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0.01% sodium azide
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抗原
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重组鼠肺表面活性蛋白C前体pro-SP-C, n-terminal (aa1-35) GST融合蛋白
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保存条件
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储存在-20℃，避免反复冻融
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产品使用
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实验应用
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建议稀释倍数
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Western Blot
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1:5000
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Immunohistochemistry
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1:3000
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说明：最佳稀释倍数与浓度应由研究人员确认
应用实例
图片为成年小鼠肺组织用抗N-末端proSP-C兔血清（1:3000稀释）染色。ProSP-C通过DAB（黑色）进行可视化，并用Nuclear Fast Red（粉红色）进行复染。放大倍数40倍。
参考文献
1. Glasser SW, Detmer EA, Ikegami M, Na CL, Stahlman MT, Whitsett JA. 'Pneumonitis and emphysema in sp-C gene targeted mice.' J Biol Chem. 2003 Apr 18;278(16):14291-8.
2. Nogee LM, Dunbar AE 3rd, Wert SE, Askin F, Hamvas A, Whitsett JA. 'A mutation in the surfactant protein C gene associated with familial interstitial lung disease.' N Engl J Med. 2001 Feb 22;344(8):573-9.
3. No abstract available. Glasser SW, Burhans MS, Korfhagen TR, Na CL, Sly PD, Ross GF, Ikegami M, Whitsett JA. 'Altered stability of pulmonary surfactant in SP-C-deficient mice.' Proc Natl Acad Sci U S A. 2001 May 22;98(11):6366-71.
4. Zhou L, Lim L, Costa RH, Whitsett JA. 'Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung.' J Histochem Cytochem. 1996 Oct;44(10):1183-93.
5. Yang, L., J. Johansson, R. Ridsdale, H. Willander, M. Fitzen, H. T. Akinbi, and T. E. Weaver. Surfactant Protein B Propeptide Contains a Saposin-Like Protein Domain with Antimicrobial Activity at Low pH. J Immunol 2010;184:975-983.
6. Conkright, J. J., K. S. Apsley, E. P. Martin, R. Ridsdale, W. R. Rice, C. L. Na, B. Yang, and T. E. Weaver. Nedd4-2-mediated ubiquitination facilitates processing of surfactant protein-C. Am J Respir Cell Mol Biol 2010;42:181-189.
7. Wang, M., J. P. Bridges, C. L. Na, Y. Xu, and T. E. Weaver. Meckel-Gruber syndrome protein MKS3 is required for endoplasmic reticulum-associated degradation of surfactant protein C. J Biol Chem 2009;284:33377-33383.
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