GFP Mouse Monoclonal Antibody
一键复制产品信息 产品编号: AF0159
产品包装:50μl
选择包装
50μl
数
量
GFP Mouse mAb(GFP小鼠单抗)(AF0159)
一键复制产品信息 说明书下载
价格: ¥ 482.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF0159 | GFP Mouse Monoclonal Antibody | 50μl | 482.00元 |
| 来源 | 用途 | 交叉反应性 | 分子量 |
| Mouse | WB, ICC, ELISA, IP, FC, IF | – | 26KDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
| 1:1000 | – | 1:100 | – | 1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | GFP Mouse Monoclonal Antibody |
| Category | Monoclonal antibody(mAb); Primary antibody |
| Isotype | IgG1 |
| Purification | ProA affinity purified |
| About the Immunogen | |
| Immunogen | This antibody is produced by immunizing mice with a recombinant protein corresponding to full length GFP. |
| Gene ID | 7011691 |
| SwissProt | P42212 |
| Synonyms | – |
| Category | Related Products |
| Background | Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF0159 | GFP Mouse Monoclonal Antibody | 50μl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
保存条件：
GFP Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
A. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
B. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
C. 回收稀释的一抗，4℃保存以备下次继续使用。
D. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：http://www.beyotime.com/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片：
 |
 |
| Fig. 1. Western blot analysis of GFP on transfected K562 with GFP using anti-GFP antibody at 1/2000 dilution. | Fig. 2. Immunocytochemistry staining GFP in transfected K562 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS. |
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| P0010 | BCA蛋白浓度测定试剂盒(增强型) | 500次 |
| P0012 | BCA蛋白浓度测定试剂盒 | 500次 |
| P0012A | SDS-PAGE凝胶配制试剂盒 | 1盒 |
| P0012AC | SDS-PAGE凝胶快速配制试剂盒 | 1盒 |
| P0013 | Western及IP细胞裂解液 | 100ml |
| P0013B | RIPA裂解液(强) | 100ml |
| P0014B | SDS-PAGE电泳液 | 10×1L |
| P0015 | SDS-PAGE蛋白上样缓冲液(5X) | 2ml |
| P0018 | BeyoECL Plus(超敏ECL化学发光试剂盒) | 共100ml |
| P0018A | BeyoECL Star(特超敏ECL化学发光试剂盒) | 共100ml |
| P0020 | 显影定影试剂盒 | 各1升 |
| P0021B | Western转膜液 | 10×1L |
| P0023A | Western一抗稀释液 | 100ml |
| P0023B | Western封闭液 | 100ml |
| P0023C6 | Western洗涤液(10X) | 10×100ml |
| P0023D | Western二抗稀释液 | 100ml |
| P0025 | Western一抗二抗去除液 | 250ml |
| P0252 | QuickBlock™ Western封闭液 | 100ml |
| P0256 | QuickBlock™ Western一抗稀释液 | 100ml |
| P0258 | QuickBlock™ Western二抗稀释液 | 100ml |
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| P0068 | 彩色预染蛋白质分子量标准 | 200μl |
| P0071 | BeyoColor™彩色预染蛋白质分子量标准 | 200μl |
| P0098 | 免疫染色固定液 | 100ml |
| P0102 | 免疫染色封闭液 | 100ml |
| P0103 | 免疫染色一抗稀释液 | 100ml |
| P0106L | 免疫染色洗涤液(10X) | 10×100ml |
| P0108 | 免疫荧光染色二抗稀释液 | 100ml |
| P0110 | 免疫染色(非荧光)二抗稀释液 | 100ml |
| P0260 | QuickBlock™免疫染色封闭液 | 100ml |
| P0262 | QuickBlock™免疫染色一抗稀释液 | 100ml |
| P0265 | QuickBlock™免疫荧光染色二抗稀释液 | 100ml |
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| FFP24 | PVDF膜(进口分装, 6.6×8.5cm, 0.2μm) | 20张/包装 |
| FFP32 | PVDF膜(进口原装，6.6×8.5cm, 0.45μm) | 20张/包装 |
| FFP51 | 转印滤纸(7.5×10cm) | 100片/包装 |
| FF057 | X-OMAT BT胶片(原柯达, 5×7英寸) | 100张/盒 |
| FFC58 | 压片暗盒(5×7英寸) | 1个 |
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搜索
相关产品请点击如下按钮：
标签抗体
使用本产品的文献：
1. Ru Yang, Yun Gao, Hui Li, Wei Huang, Dezhen Tu, Mengnan Yang, Xingqian Liu, Jau-Shyong Hong, Hui-Ming Gao
Posttranslational S-nitrosylation modification regulates HMGB1 secretion and promotes its proinflammatory and neurodegenerative effects
Cell Rep. 2022 Sep 13;40(11):111330.;doi: 10.1016/j.celrep.2022.111330. (IF 8.109)
2. Wenhui Li, Zilin Zhang, Liangliang Zhang, Hong Li, Shuangqi Fan, Erpeng Zhu, Jindai Fan, Zhaoyao Li, Wenxian Chen, Lin Yi, Hongxing Ding, Jinding Chen, Mingqiu Zhao
Antiviral Role of Serine Incorporator 5 (SERINC5) Proteins in Classical Swine Fever Virus Infection
Front Microbiol. 2020 Sep 4;11:580233.;doi: 10.3389/fmicb.2020.580233. (IF 4.235)
HA Tag Mouse mAb (HRP Conjugated)(HA Tag 小鼠单抗)(AF2861-1ml)
HA Tag Mouse Monoclonal Antibody (HRP Conjugated)
一键复制产品信息 产品编号: AF2861-1ml
产品包装:1ml
选择包装
50μl 200μl 1ml
数
量
说明书下载
价格: ¥ 4586.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2861-50μl | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl | 388.00元 |
| AF2861-200μl | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 200μl | 1168.00元 |
| AF2861-1ml | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 1ml | 4586.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IHC, ICC | HA-tagged protein in all species | – | – |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | – | – | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) |
| Category | Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | 293T |
| Cellular location | – |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Synthetic HA tag peptide coupled to KLH |
| Sequence | YPYDVPDYA |
| Gene ID | – |
| SwissProt | – |
| Synonyms | YPYDVPDYA; hemagglutinin |
| Category | Polypeptide protein tag |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. The HA tag is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2861-50µl | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2861-200µl | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 200µl |
| AZ200 | Western一抗稀释液 | 200ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2861-1ml | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 1ml |
| AZ502 | Western一抗稀释液 | AZ500×2 |
| — | 说明书 | 1份 |
保存条件：
HA Tag Mouse Monoclonal Antibody (HRP Conjugated) -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
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| AF2891 | GST Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
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| AF2909 | DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
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| AF2915 | MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
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| AF2924 | StrepII Tag Mouse Monoclonal Antibody | 50μl |
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| AF2933 | S Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2936 | TEV-His Tag Mouse Monoclonal Antibody | 50μl |
| AF2939 | TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2942 | Target Tag Mouse Monoclonal Antibody | 50μl |
| AF2945 | Target Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2948 | TRX Tag Mouse Monoclonal Antibody | 50μl |
| AF2951 | TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2954 | AVI Tag Mouse Monoclonal Antibody | 50μl |
| AF2957 | AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
质检证书
输入批号搜寻COA
搜索
相关产品请点击如下按钮：
标签抗体
HRP标记二抗
HRP标记一抗
使用本产品的文献：
β-Actin Mouse mAb (HRP Conjugated)(β-Actin 小鼠单抗)(AF2815-1ml)
β-Actin Mouse Monoclonal Antibody (HRP Conjugated)
一键复制产品信息 产品编号: AF2815-1ml
产品包装:1ml
选择包装
50μl 200μl 1ml
数
量
说明书下载
价格: ¥ 4586.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2815-50μl | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) | 50μl | 388.00元 |
| AF2815-200μl | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) | 200μl | 1168.00元 |
| AF2815-1ml | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) | 1ml | 4586.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IHC, ICC | All | 43kDa | 43kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | – | – | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) |
| Category | Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | HeLa, TE-1, 293T, NIH/3T3, Mouse brain, Mouse testis, C6, Rat brain, Rat lung |
| Cellular location | Cytoplasm, cytoskeleton |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Synthetic peptide DDIAALVIDNGSG coupled to KLH |
| Sequence | DDIAALVIDNGSG |
| Gene ID | 60/11461/81822 |
| SwissProt | P60709/P60710/P60711 |
| Synonyms | Act; Actx; beta-a; beta-actin; E430023M04Rik |
| Category | Cytoskeletal Signaling |
| Background | Beta actin also known as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β-Actin is generally used as an internal control for experimental error. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2815-50µl | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2815-200µl | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) | 200µl |
| AZ200 | Western一抗稀释液 | 200ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2815-1ml | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) | 1ml |
| AZ502 | Western一抗稀释液 | AZ500×2 |
| — | 说明书 | 1份 |
保存条件：
β-Actin Mouse Monoclonal Antibody (HRP Conjugated) -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作，无须再孵育二抗。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作，无须再孵育二抗。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。
相关产品：
| 产品编号 | 产品名称 | 包装 |
| AF2811 | β-Actin Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2815 | β-Actin Mouse Monoclonal Antibody(HRP Conjugated) | 50μl/200μl/1ml |
| AF2819 | GAPDH Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2823 | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2827 | α-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2831 | α-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2835 | β-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2839 | β-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
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HRP标记一抗
使用本产品的文献：
β-Actin Mouse mAb(β-Actin小鼠单抗)(AF0003)
β-Actin Mouse Monoclonal Antibody
一键复制产品信息 产品编号: AF0003
产品包装:100μl
选择包装
100μl
数
量
说明书下载
价格: ¥ 446.00
产品简介
使用说明
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产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF0003 | β-Actin Mouse Monoclonal Antibody | 100μl | 446.00元 |
| 来源 | 用途 | 交叉反应性 | 分子量 |
| Mouse | WB, ICC, IHC, IF | H, M, R | 43KDa |
WB,Western blot;IP,Immunoprecipitation;IF,Immunofluorescence;IHC,Immunohistochemistry;ICC,Immunocytochemistry;FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
| 1:1000 | – | 1:20 | 1:20 | 1:20 | – | – | – |
抗体详细信息如下:
|
About this Antibody |
|
|
Name |
β-Actin Mouse Monoclonal Antibody |
|
Category |
Monoclonal antibody (mAb); Primary antibody |
|
Isotype |
IgG1 |
|
Purification |
Affinity chromatography |
|
About the Immunogen |
|
|
Immunogen |
synthetic peptide (KLH-coupled) |
|
Gene ID |
60/11461/81822 |
|
SwissProt |
P60709/P60710/P60711 |
|
Synonyms |
BRWS1; PS1TP5BP1 |
|
Category |
Cytoskeletal Signaling |
|
Background |
Beta actin also know as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β-Actin is generally used as an internal control for experimental error. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF0003 | β-Actin Mouse Monoclonal Antibody | 100μl |
| AZ100 | Western一抗稀释液 | 100ml |
| — | 说明书 | 1份 |
保存条件：
β-Actin Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋
白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体 操作可以参考如下网页： http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片:
Fig. 1. Western blot analysis on Hela (A), NIH/3T3 (B) and PC12 (C) cell lysatesusing β-actin mousemonoclonal antibody (AF003).
Fig. 2. Immunofluorescent staining of NIH/3T3 cells using β-actin mouse monoclonalantibody (AF0003). Nucleus (blue) were stained with DAPI.
Fig. 3. Immunohistochemical analysis of paraffin-embedded mouse testis tissueusing β-actin mouse monoclonal antibody (AF0003).
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质检证书
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搜索
相关产品请点击如下按钮：
内参抗体
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Int J Mol Med. 2016 Oct;38(4):1152-60. (IF 3.098)
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101. Yingge Liu, Meiying Zhang, Tao He, Weili Yang, Lidong Wang, Lirong Zhang, Mingzhou Guo
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Leptin Influence Cholelithiasis Formation by Regulating Bile Acid Metabolism
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GAPDH Mouse mAb(GAPDH小鼠单抗)(AF0006)
GAPDH Mouse Monoclonal Antibody
一键复制产品信息 产品编号: AF0006
产品包装:100μl
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100μl
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价格: ¥ 449.00
产品简介
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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF0006 | GAPDH Mouse Monoclonal Antibody | 100μl | 449.00元 |
| 来源 | 用途 | 交叉反应性 | 分子量 |
| Mouse | WB, ICC, IF | H, M, R, C, Dg, Gp,Hm, Mk, Pg, Rb, S,Yeast | 36KDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
| 1:1000 | – | 1:100 | 1:50 | 1:50 | – | – | – |
抗体详细信息如下:
|
About this Antibody |
|
|
Name |
GAPDH Mouse Monoclonal Antibody |
|
Category |
Monoclonal antibody (mAb); Primary antibody |
|
Isotype |
IgG1 |
|
Purification |
Affinity purification |
|
About the Immunogen |
|
|
Immunogen |
synthetic peptide (KLH-coupled) |
|
Gene ID |
2597/14433/24383 |
|
SwissProt |
P04406/P16858/P04797 |
|
Synonyms |
GAPDH; G3PD; GAPD; MGC88685 |
|
Category |
Metabolism |
|
Background |
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF0006 | GAPDH Mouse Monoclonal Antibody | 100μl |
| AZ100 | Western一抗稀释液 | 100ml |
| — | 说明书 | 1份 |
保存条件：
GAPDH Mouse Monoclonal Antibody-20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋
白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页： http://www.beyotime.com/suppport/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片:
 |
 |
Fig. 1. Western blot analysis on HeLa (1) and NIH/3T3 (2) cell lysates using GAPDHmouse monoclonal antibody (AF0006).
Fig. 2. ICC staining GAPDH in D3 cells (red). Cells were fixed in paraformaldehyde,permeabilized with PBS containing 0.25% Triton X-100 and counterstained with DAPIto visualize the nucleus (blue).
Fig. 3. ICC staining GAPDH in A431 cells (red). Cells were fixed inparaformaldehyde, permeabilized with PBS containing 0.25% Triton X-100 andcounterstained with DAPI to visualize the nucleus (blue).
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| P0106L | 免疫染色洗涤液(10X) | 10×100ml |
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14. Lan Jin, Yunhe Chen, Dan Cheng, Zhikai He, Xinyi Shi, Boyu Du, Xueyan Xi, Yujing Gao, Yang Guo
YAP inhibits autophagy and promotes progression of colorectal cancer via upregulating Bcl-2 expression
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Bone. 2018 May;110:47-57. (IF 4.147)
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J STROKE CEREBROVASC . 2019 May;28(5):1338-1345. (IF 1.598)
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VIRUSES-BASEL. 2019 Oct 8;11(10). pii: E920. (IF 3.816)
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FRONT NEUROSCI-SWITZ. 2020 Jan 31;14:31.;doi: 10.3389/fnins.2020.00031. (IF 3.707)
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DNA Cell Biol. 2020 Feb;39(2):310-320.;doi: 10.1089/dna.2019.4966. (IF 3.191)
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Ann Transl Med. 2020 Mar;8(5):218.;doi: 10.21037/atm.2020.01.14. (IF 3.297)
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Oncol Rep. 2020 May;43(5):1375-1386.;doi: 10.3892/or.2020.7515. (IF 3.417)
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A Human iPSC Line Carrying a de novo Pathogenic FUS Mutation Identified in a Patient With Juvenile ALS Differentiated Into Motor Neurons With Pathological Characteristics
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Effect of Human Umbilical Cord Mesenchymal Stem Cells Transfected with HGF on TGF-β1/Smad Signaling Pathway in Carbon Tetrachloride-Induced Liver Fibrosis Rats
Stem Cells Dev. 2020 Nov 1;29(21):1395-1406.;doi: 10.1089/scd.2020.0060. (IF 3.082)
109. Shichao Zhuang, Xiaohui Yu, Ming Lu, Yujiao Li, Ning Ding, Yumei Ding
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A genetic variant in the promoter of CD46 is associated with the risk and prognosis of hepatocellular carcinoma
MOL CARCINOGEN. 2020 Nov;59(11):1243-1255.;doi: 10.1002/mc.23252. (IF 3.825)
112. Xiao Liu, Furong Xie, Guangyun Lai, Jun Wang
Roles of heterogeneous nuclear ribonucleoprotein L in enamel organ development and the differentiation of ameloblasts
Arch Oral Biol. 2020 Dec;120:104933.;doi: 10.1016/j.archoralbio.2020.104933. (IF 1.931)
113. Haiyu Zhang, Tongshuai Zhang, Dandan Wang, Yixiang Jiang, Tieyun Guo, Yao Zhang, Fan Zhu, Kaiyu Han, Lili Mu, Guangyou Wang
IFN-γ regulates the transformation of microglia into dendritic-like cells via the ERK/c-myc signaling pathway during cerebral ischemia/reperfusion in mice
Neurochem Int. 2020 Dec;141:104860.;doi: 10.1016/j.neuint.2020.104860. (IF 3.881)
114. Xin Xue, Liang Zhang, Xiang Yin, Xing-Xing Chen, Zong-Feng Chen, Chen-Xu Wang, Yu Xiang, Ming-Yong Liu, Jian-Hua Zhao
Transplantation of neural stem cells preconditioned with high‑mobility group box 1 facilitates functional recovery after spinal cord injury in rats
Mol Med Rep. 2020 Dec;22(6):4725-4733.;doi: 10.3892/mmr.2020.11565. (IF 2.1)
115. Yongfeng Li, Jin Zong, Cong Zhao
lncRNA CTBP1-AS2 promotes proliferation and migration of glioma by modulating miR-370-3p-Wnt7a-mediated epithelial-mesenchymal transition
Biochem Cell Biol. 2020 Dec;98(6):661-668.;doi: 10.1139/bcb-2020-0065. (IF 2.46)
116. Ning Ma, Xingjie Li, Hongyu Jiang, Yulong Dai, Guofeng Xu, Zongde Zhang
Influenza Virus Neuraminidase Engages CD83 and Promotes Pulmonary Injury
J Virol. 2021 Jan 13;95(3):e01753-20.;doi: 10.1128/JVI.01753-20. (IF 4.501)
117. Shasha Yao, Wenwen Shang, Lei Huang, Rui Xu, Ming Wu, Fang Wang
The oncogenic and prognostic role of PDK1 in the progression and metastasis of ovarian cancer
J Cancer. 2021 Jan 1;12(3):630-643.;doi: 10.7150/jca.47278. (IF 3.565)
118. Hongfei Ge, Chao Zhang, Yang Yang, Weixiang Chen, Jun Zhong, Xuanyu Fang, Xuheng Jiang, Liang Tan, Yongjie Zou, Rong Hu, Yujie Chen, Hua Feng
Ambroxol Upregulates Glucocerebrosidase Expression to Promote Neural Stem Cells Differentiation Into Neurons Through Wnt/β-Catenin Pathway After Ischemic Stroke
Front Mol Neurosci. 2021 Jan 20;13:596039.;doi: 10.3389/fnmol.2020.596039. (IF 4.057)
119. Huihui Xu, Song Dun, Ying Gao, Jian Ming, Linping Hui, Xueshan Qiu
TMEM107 inhibits EMT and invasion of NSCLC through regulating the Hedgehog pathway
Thorac Cancer. 2021 Jan;12(1):79-89.;doi: 10.1111/1759-7714.13715. (IF 2.61)
120. Guangzhao Song, Liyan Li, Ying Yang
MicroRNA-329-3p alleviates high glucose-induced endothelial cell injury via inhibition of the TLR4/TRAF6/NF-κB signaling pathway
Exp Ther Med. 2021 Jan;21(1):29.;doi: 10.3892/etm.2020.9461. (IF 1.785)
121. Haifeng Jin, Yu Jiao, Linna Guo, Yong Ma, Rongjie Zhao, Xuemei Li, Lei Shen, Zhongguang Zhou, Sang Chan Kim, Jicheng Liu
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Int J Mol Med. 2021 Feb;47(2):595-606.;doi: 10.3892/ijmm.2020.4813. (IF 3.098)
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Cell Tissue Res. 2021 Mar;383(3):1155-1165.;doi: 10.1007/s00441-020-03338-y. (IF 4.044)
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Mol Ther Methods Clin Dev. 2021 Jun 24;22:293-303.;doi: 10.1016/j.omtm.2021.06.009.
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GAPDH Mouse mAb (HRP Conjugated)(GAPDH 小鼠单抗)(AF2823-1ml)
GAPDH Mouse Monoclonal Antibody (HRP Conjugated)
一键复制产品信息 产品编号: AF2823-1ml
产品包装:1ml
选择包装
50μl 200μl 1ml
数
量
说明书下载
价格: ¥ 4586.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2823-50μl | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 50μl | 388.00元 |
| AF2823-200μl | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 200μl | 1168.00元 |
| AF2823-1ml | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 1ml | 4586.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IHC, ICC | All | 31/36kDa | 36kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | – | – | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) |
| Category | Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | HeLa, Jurkat, 293T, NIH/3T3, Raw 264.7, Mouse brain, Rat brain, Rat heart, Rat liver |
| Cellular location | Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Synthetic peptide CRLEKPAKYDDIKK coupled to KLH |
| Sequence | CRLEKPAKYDDIKK |
| Gene ID | 2597 |
| SwissProt | P04406 |
| Synonyms | GAPDH; G3PD; GAPD; HEL-S-162eP; glyceraldehyde-3-phosphate dehydrogenase |
| Category | Metabolism |
| Background | GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2823-50µl | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2823-200µl | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 200µl |
| AZ200 | Western一抗稀释液 | 200ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2823-1ml | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 1ml |
| AZ502 | Western一抗稀释液 | AZ500×2 |
| — | 说明书 | 1份 |
保存条件：
GAPDH Mouse Monoclonal Antibody (HRP Conjugated) -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作，无须再孵育二抗。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作，无须再孵育二抗。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。
相关产品：
| 产品编号 | 产品名称 | 包装 |
| AF2811 | β-Actin Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2815 | β-Actin Mouse Monoclonal Antibody(HRP Conjugated) | 50μl/200μl/1ml |
| AF2819 | GAPDH Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2823 | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2827 | α-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2831 | α-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2835 | β-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2839 | β-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
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相关产品请点击如下按钮：
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使用本产品的文献：
GAPDH Mouse mAb(GAPDH 小鼠单抗)(AF2819-1ml)
GAPDH Mouse Monoclonal Antibody
一键复制产品信息 产品编号: AF2819-1ml
产品包装:1ml
选择包装
50μl 200μl 1ml
数
量
说明书下载
价格: ¥ 3528.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2819-50μl | GAPDH Mouse Monoclonal Antibody | 50μl | 299.00元 |
| AF2819-200μl | GAPDH Mouse Monoclonal Antibody | 200μl | 899.00元 |
| AF2819-1ml | GAPDH Mouse Monoclonal Antibody | 1ml | 3528.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IF, IHC, ICC | All | 31/36kDa | 36kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | – | 1:50-1:100 | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | GAPDH Mouse Monoclonal Antibody |
| Category | Mouse Monoclonal Antibody (mAb); Primary antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | HeLa, Jurkat, 293T, NIH/3T3, Raw 264.7, Mouse brain, Rat brain, Rat heart, Rat liver |
| Cellular location | Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Synthetic peptide CRLEKPAKYDDIKK coupled to KLH |
| Sequence | CRLEKPAKYDDIKK |
| Gene ID | 2597 |
| SwissProt | P04406 |
| Synonyms | GAPDH; G3PD; GAPD; HEL-S-162eP; glyceraldehyde-3-phosphate dehydrogenase |
| Category | Metabolism |
| Background | GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2819-50µl | GAPDH Mouse Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2819-200µl | GAPDH Mouse Monoclonal Antibody | 200µl |
| AZ200 | Western一抗稀释液 | 200ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2819-1ml | GAPDH Mouse Monoclonal Antibody | 1ml |
| AZ502 | Western一抗稀释液 | AZ500×2 |
| — | 说明书 | 1份 |
保存条件：
GAPDH Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
| 产品编号 | 产品名称 | 包装 |
| AF2811 | β-Actin Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2815 | β-Actin Mouse Monoclonal Antibody(HRP Conjugated) | 50μl/200μl/1ml |
| AF2819 | GAPDH Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2823 | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2827 | α-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2831 | α-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2835 | β-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2839 | β-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
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相关产品请点击如下按钮：
内参抗体
使用本产品的文献：
1. Dongya Zhang, Sidan Tian, Yanjie Liu, Meng Zheng, Xiangliang Yang, Yan Zou, Bingyang Shi, Liang Luo
Near infrared-activatable biomimetic nanogels enabling deep tumor drug penetration inhibit orthotopic glioblastoma
Nat Commun. 2022 Nov 11;13(1):6835.;doi: 10.1038/s41467-022-34462-8. (IF 12.121)
β-Tubulin Mouse mAb(β-Tubulin 小鼠单抗)(AF2835-1ml)
β-Tubulin Mouse Monoclonal Antibody
一键复制产品信息 产品编号: AF2835-1ml
产品包装:1ml
选择包装
50μl 200μl 1ml
数
量
说明书下载
价格: ¥ 3528.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2835-50μl | β-Tubulin Mouse Monoclonal Antibody | 50μl | 299.00元 |
| AF2835-200μl | β-Tubulin Mouse Monoclonal Antibody | 200μl | 899.00元 |
| AF2835-1ml | β-Tubulin Mouse Monoclonal Antibody | 1ml | 3528.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IF, IHC, ICC | All | ~50kDa | 50-55kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | – | 1:50-1:100 | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | β-Tubulin Mouse Monoclonal Antibody |
| Category | Mouse Monoclonal Antibody (mAb); Primary antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | – |
| Cellular location | – |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Synthetic peptide ESNMNDLVSEYQQYQDATAC coupled to KLH |
| Sequence | ESNMNDLVSEYQQYQDATAC |
| Gene ID | 203068 |
| SwissProt | P07437 |
| Synonyms | M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56 |
| Category | Cytoskeletal Signaling |
| Background | Tubulin is the major building block of microtubules. This intracellular,cylindrical,filamentous structure is present in almost all eukaryoticcells.Tubulin is a heterodimer that consists of α-tubulin and β-tubulin. Our anti-β-tubulin monoclonal antibody provides a specific and useful tool in studying the intracellular distribution of tubulin and the static and dynamic aspects of the cytoskeleton. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2835-50µl | β-Tubulin Mouse Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2835-200µl | β-Tubulin Mouse Monoclonal Antibody | 200µl |
| AZ200 | Western一抗稀释液 | 200ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2835-1ml | β-Tubulin Mouse Monoclonal Antibody | 1ml |
| AZ502 | Western一抗稀释液 | AZ500×2 |
| — | 说明书 | 1份 |
保存条件：
β-Tubulin Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
| 产品编号 | 产品名称 | 包装 |
| AF2811 | β-Actin Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2815 | β-Actin Mouse Monoclonal Antibody(HRP Conjugated) | 50μl/200μl/1ml |
| AF2819 | GAPDH Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2823 | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2827 | α-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2831 | α-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2835 | β-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2839 | β-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
质检证书
输入批号搜寻COA
搜索
相关产品请点击如下按钮：
内参抗体
使用本产品的文献：
β-Actin Mouse mAb(β-Actin 小鼠单抗)(AF2811-1ml)
β-Actin Mouse Monoclonal Antibody
一键复制产品信息 产品编号: AF2811-1ml
产品包装:1ml
选择包装
50μl 200μl 1ml
数
量
说明书下载
价格: ¥ 3528.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2811-50μl | β-Actin Mouse Monoclonal Antibody | 50μl | 299.00元 |
| AF2811-200μl | β-Actin Mouse Monoclonal Antibody | 200μl | 899.00元 |
| AF2811-1ml | β-Actin Mouse Monoclonal Antibody | 1ml | 3528.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IF, IHC, ICC | All | 43kDa | 43kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | – | 1:50-1:100 | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | β-Actin Mouse Monoclonal Antibody |
| Category | Mouse Monoclonal Antibody (mAb); Primary antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | HeLa, TE-1, 293T, NIH/3T3, Mouse brain, Mouse testis, C6, Rat brain, Rat lung |
| Cellular location | Cytoplasm, cytoskeleton |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Synthetic peptide DDIAALVIDNGSG coupled to KLH |
| Sequence | DDIAALVIDNGSG |
| Gene ID | 60/11461/81822 |
| SwissProt | P60709/P60710/P60711 |
| Synonyms | Act; Actx; beta-a; beta-actin; E430023M04Rik |
| Category | Cytoskeletal Signaling |
| Background | Beta actin also known as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β-Actin is generally used as an internal control for experimental error. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2811-50µl | β-Actin Mouse Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2811-200µl | β-Actin Mouse Monoclonal Antibody | 200µl |
| AZ200 | Western一抗稀释液 | 200ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2811-1ml | β-Actin Mouse Monoclonal Antibody | 1ml |
| AZ502 | Western一抗稀释液 | AZ500×2 |
| — | 说明书 | 1份 |
保存条件：
β-Actin Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
| 产品编号 | 产品名称 | 包装 |
| AF2811 | β-Actin Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2815 | β-Actin Mouse Monoclonal Antibody(HRP Conjugated) | 50μl/200μl/1ml |
| AF2819 | GAPDH Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2823 | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2827 | α-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2831 | α-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2835 | β-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2839 | β-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
质检证书
输入批号搜寻COA
搜索
相关产品请点击如下按钮：
内参抗体
使用本产品的文献：
1. Fangyuan Li, Heng Sun, Jiafeng Ren, Bo Zhang, Xi Hu, Chunyan Fang, Jiyoung Lee, Hongzhou Gu, Daishun Ling
A nuclease-mimetic platinum nanozyme induces concurrent DNA platination and oxidative cleavage to overcome cancer drug resistance
Nat Commun. 2022 Nov 30;13(1):7361.;doi: 10.1038/s41467-022-35022-w. (IF 12.121)
α-Tubulin Mouse mAb(α-Tubulin 小鼠单抗)(AF2827-1ml)
α-Tubulin Mouse Monoclonal Antibody
一键复制产品信息 产品编号: AF2827-1ml
产品包装:1ml
选择包装
50μl 200μl 1ml
数
量
说明书下载
价格: ¥ 3528.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2827-50μl | α-Tubulin Mouse Monoclonal Antibody | 50μl | 299.00元 |
| AF2827-200μl | α-Tubulin Mouse Monoclonal Antibody | 200μl | 899.00元 |
| AF2827-1ml | α-Tubulin Mouse Monoclonal Antibody | 1ml | 3528.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IF, IHC, ICC | All | ~50kDa | 50-55kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | – | 1:50-1:100 | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | α-Tubulin Mouse Monoclonal Antibody |
| Category | Mouse Monoclonal Antibody (mAb); Primary antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | – |
| Cellular location | – |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Synthetic peptide CAALEKDYEEVGVDSVEGEGEEEGEE coupled to KLH |
| Sequence | CAALEKDYEEVGVDSVEGEGEEEGEE |
| Gene ID | 22142 |
| SwissProt | P68369 |
| Synonyms | Tuba; Tuba-; Tuba1; Tuba-1 |
| Category | Cytoskeletal Signaling |
| Background | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular Tubulin subunits comprise the microtubule building block, with α/β-Tubulin heterodimers forming the Tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2827-50µl | α-Tubulin Mouse Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2827-200µl | α-Tubulin Mouse Monoclonal Antibody | 200µl |
| AZ200 | Western一抗稀释液 | 200ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2827-1ml | α-Tubulin Mouse Monoclonal Antibody | 1ml |
| AZ502 | Western一抗稀释液 | AZ500×2 |
| — | 说明书 | 1份 |
保存条件：
α-Tubulin Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
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| AF2811 | β-Actin Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2815 | β-Actin Mouse Monoclonal Antibody(HRP Conjugated) | 50μl/200μl/1ml |
| AF2819 | GAPDH Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2823 | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2827 | α-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2831 | α-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2835 | β-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2839 | β-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
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