Biognosys iRT Kit——蛋白质谱利器
indexed retention time (iRT)是定义一个肽在特定类型填料(如C18)中的色谱保留时间相对于iRT标准的无纲量值。和经验保留时间不同,一个肽的iRT值是稳定的并且可以用来在任何色谱条件下精确预测肽的保留时间。
iRT标准是11种自然中不存在的合成肽段的混合物。这些肽段经过仔细地稳定性、灵敏度甚至不同梯度下保留时间间隔的优化。通过iRT试剂盒可以直接测定肽段的iRT值并用来校准色谱系统。
使用iRT试剂盒校准您的LC系统后,通过带入更多的transition,可以大幅提高您靶向蛋白质组(MRM和PRM)实验的通量,甚至可达20倍。iRT标准带来的好处对下一代蛋白质组学研究流程如DIA或SWATH来说同样重要。iRT肽段可以在您的DIA或SWATH检测中使高质量图谱库的生成及精确归一化成为可能。
iRT肽段需要与Biognosys软件同时使用:Spectronaut用于DIA或SWATH数据分析,SpectroDive用于靶向蛋白质组学实验。
蛋白质谱
蛋白质谱技术是研究蛋白质组学中新的一项技术,它是通过蛋白酶将蛋白质酶切消化后形成肽段混合物,在质谱仪中将其电离形成带电离子,并通过特定的质荷比(m/z),进而形成一级和二级质谱,从而定量和鉴定蛋白。而在这的过程中,激发图谱库以及校准肽保留时间(Retention Time)尤为重要。
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iRT kit
iRT-indexed Retention Time是定义一个肽在特定类型填料(如C18)中的色谱保留时间相对于iRT标准的无纲量值。和传统的保留时间不同,一个肽的iRT值是稳定的并且可以用来在任何色谱条件下精确预测肽的保留时间。
iRT标准品是11种自然中不存在的合成肽的混合物。这些肽段经过特定地稳定性、灵敏度甚至不同梯度下保留时间间隔的优化。通过iRT试剂盒可以直接测定肽段的iRT值,能校准色谱系统并准确预测目标肽的保留时间。
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使用步骤
iRT试剂盒用于时间分辨质谱经过两个步骤:
1. 测定目标肽段的iRT值与iRT标准品之间的关系
2. 使用iRT标准品校准LC系统,并准确预测目标肽段的保留时间
使用及包装
1x iRT试剂盒包含:
• Dissolution Buffer (1x 2.0 ml tube)
• iRT Standard (1x 2.0 ml tube)
使用次数取决于样品量及进样量,试剂盒适用于500次进样或50个样品。
Biognosys2022年价格表
货号 | 品名 | 规格 | 价格 | 品牌 |
Ki-3002-1 | iRT Kit | 1Kit | 2772.00 | Biognosys |
Ki-3002-2 | 2xiRT Kit | 1Kit | 5320.00 | Biognosys |
Ki-3016-48 | Plasma Dive Reference Peptides 48 | 1Kit | 38500.00 | Biognosys |
Ki-3016-96 | Plasma Dive Reference Peptides 96 | 1Kit | 70000.00 | Biognosys |
Ki-3017-48 | Plasma Deep Dive Reference Peptides 48 | 1Kit | 38500.00 | Biognosys |
Ki-3017-96 | Plasma Deep Dive Reference Peptides 96 | 1Kit | 70000.00 | Biognosys |
Ki-3019-24 | PQ500 Reference Peptides 24 | 1Kit | 45500.00 | Biognosys |
Ki-3019-48 | PQ500 Reference Peptides 48 | 1Kit | 77700.00 | Biognosys |
Ki-3019-96 | PQ500 Reference Peptides 96 | 1Kit | 136500.00 | Biognosys |
Ki-3010 | Sample Preparation Kit | 1Kit | 4200.00 | Biognosys |
Ki-3013 | Sample Preparation Kit Pro | 1Kit | 13720.00 | Biognosys |
Sw-3001-a | Spectronaut X- 1 Year Single System License [academic] | ea | 84000.00 | Biognosys |
Sw-3001-a-m | Spectronaut X – 1 Year Multi System License [academic] | ea | 196000.00 | Biognosys |
Sw-3001-a-p | Spectronaut X – Perpetual Single System License [academic] | ea | 168000.00 | Biognosys |
Sw-3001-a-s | 1 Year Subscription to Biognosys Support Plan for Spectronaut X [academic] | ea | 42000.00 | Biognosys |
Sw-3001 | Spectronaut X – 1 Year Single System License [non academic] | ea | 210000.00 | Biognosys |
Sw-3001-m | Spectronaut X – 1 Year Multi System License [non academic] | ea | 588000.00 | Biognosys |
Sw-3001-p | Spectronaut X – Perpetual Single System License [non academic] | ea | 406000.00 | Biognosys |
Sw-3001-s | 1 Year Subscription to Biognosys Support Plan for Spectronaut X [non academic] | ea | 105000.00 | Biognosys |
Sw-3001-ac | Spectronaut X – 1 Year Single System License [academic core facility] | ea | 133000.00 | Biognosys |
Sw-3001-ac-p | Spectronaut X – Perpetual Single Systems License [academic core facility] | ea | 266000.00 | Biognosys |
Sw-3001-ac-s | 1 Year Subscription to Biognosys Support Plan for Spectronaut X [academic core facility] | ea | 66500.00 | Biognosys |
Sw-3004-a | SpectroMine – 1 Year Single System License [academic] | ea | 70000.00 | Biognosys |
Sw-3004-a-m | SpectroMine – 1 Year Multi System Site License [academic] | ea | 168000.00 | Biognosys |
Sw-3004-a-p | SpectroMine – Perpetual Single Systems License [academic] | ea | 154000.00 | Biognosys |
Sw-3004-a-s | 1 Year Subscription to Biognosys Support Plan for SpectroMine [academic] | ea | 35000.00 | Biognosys |
Sw-3004 | SpectroMine – 1 Year Single System License [non academic] | ea | 196000.00 | Biognosys |
Sw-3004-m | SpectroMine – 1 Year Multi System Site License [non academic] | ea | 560000.00 | Biognosys |
Sw-3004-p | SpectroMine – Perpetual Single Systems License [non academic] | ea | 392000.00 | Biognosys |
Sw-3004-s | 1 Year Subscription to Biognosys Support Plan for SpectroMine [non academic] | ea | 98000.00 | Biognosys |
Sw-3004-ac | SpectroMine – 1 Year Single System License [academic core facility] | ea | 119000.00 | Biognosys |
Sw-3004-ac-p | SpectroMine – Perpetual Single Systems License [academic core facility] | ea | 252000.00 | Biognosys |
Sw-3004-ac-s | 1 Year Subscription to Biognosys Support Plan for SpectroMine [academic core facility] | ea | 56000.00 | Biognosys |
Sw-3002-a | Spectro Dive – 1 Year Single Systems License [academic] | ea | 63000.00 | Biognosys |
Sw-3002-a-m | Spectro Dive – 1 Year Multi Systems License [academic] | ea | 154000.00 | Biognosys |
Sw-3002-a-p | Spectro Dive – Perpetual Single System License [academic] | ea | 126000.00 | Biognosys |
SW-3002-a-s | 1 Year Subscription to Biognosys Support Plan for Spectro Dive [academic] | ea | 35000.00 | Biognosys |
Sw-3002 | Spectro Dive – 1 Year Single Systems License [non academic] | ea | 168000.00 | Biognosys |
Sw-3002-m | Spectro Dive – 1 Year Multi Systems License [non academic] | ea | 364000.00 | Biognosys |
Sw-3002-p | Spectro Dive – Perpetual Single System License [non academic] | ea | 280000.00 | Biognosys |
SW-3002-s | 1 Year Subscription to Biognosys Support Plan for Spectro Dive [non academic] | ea | 70000.00 | Biognosys |
Sw-3002-ac | SpectroDive – 1 Year Single System License [academic core facility] | ea | 105000.00 | Biognosys |
Sw-3002-ac-p | SpectroDive – Perpetual Single System License [academic core facility] | ea | 196000.00 | Biognosys |
Sw-3004-ac-s | 1 Year Subscription to Biognosys Support Plan for SpectroDive [academic core facility] | ea | 47600.00 | Biognosys |