产品描述

细胞裂解后的蛋白粗提物中含有大量的内源性蛋白酶，这些酶可以降解细胞提取物中的蛋白。因此为了防止蛋白纯化过程中目的蛋白不受蛋白酶的破坏，需要加入蛋白酶抑制剂对其进行活性抑制。

本品是多种常见蛋白酶抑制剂的混合物，包含AEBSF、Aprotinin、Bestatin、E-64、Leupeptin、Pepstatin A多种成分，广谱抑制来源于动植物、细菌、酵母等组织或细胞内的多种蛋白酶，如丝氨酸蛋白酶、半胱氨酸蛋白酶、氨肽酶、酸性蛋白酶等。另外，本品不含EDTA（一种金属蛋白酶抑制剂），特别适用于活性中心为金属离子依赖的蛋白。兼容于后续的固定化金属离子亲和层析（Immobilized metal-chelate affinity chromatography, IMAC）、2-D 凝胶电泳以及天然凝胶电泳等蛋白分析实验。

本品为溶于DMSO的溶液，以100×储存液的形式提供，可用于WB，Co-IP，Pull-down assay，IF，IHC，激酶研究等多种分析。

使用方法

使用前将本品取出回温至室温，样品分析前按照1:100（v/v）的比例加入到样品溶液中，混匀即可。

运输和保存方法

冰袋运输。-20℃保存，1年有效。

注意事项

1）本品具有广谱的蛋白酶抑制活性，但是不具有磷酸酶抑制剂活性。若进行磷酸化蛋白的研究，可使用我司的磷酸酶抑制剂混合物（100×）（Cat#20109 & Cat#20110）。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

3）本产品仅作科研用途！

可能遇到的问题

Q：该产品作用范围是什么？

A：广谱抑制来源于动植物、细菌、酵母等组织或细胞内的丝氨酸蛋白酶、半胱氨酸蛋白酶、氨肽酶、酸性蛋白酶等。

Q：该产品的有何特点？

A：本品不含 EDTA，特别适用于活性中心为金属离子依赖的蛋白；兼容后续的固定化金属离子亲和层析凝胶电泳及天然凝胶电泳等蛋白分析实验。

Q：该产品与 20123 有何不同？

A：20124 成分与 20123 相同，以溶于DMSO 的溶液形式提供。二者均不含EDTA，EDTA 为金属蛋白酶抑制剂。区别在于 20123 为片剂，20124 为储液。

Q：怎么使用，和罗氏一样吗？

A：使用方法建议参考说明书。简便程度与罗氏相同，效果与罗氏无差别。已经有超过400 家实验室在使用。

[1] Xiang Y, Bian X, Wei T, et al. ZmMPK5 phosphorylates ZmNAC49 to enhance oxidative stress tolerance in maize. New Phytol. 2021;232(6):2400-2417. doi:10.1111/nph.17761(IF:10.152)
[2] Liu J, Yang Y, Liu X, Widjaya AS, Jiang B, Jiang Y. Macrophage-biomimetic anti-inflammatory liposomes for homing and treating of aortic dissection. J Control Release. 2021;337:224-235. doi:10.1016/j.jconrel.2021.07.032(IF:9.776)
[3] Liu L, Li Y, Cao D, et al. SIRT3 inhibits gallbladder cancer by induction of AKT-dependent ferroptosis and blockade of epithelial-mesenchymal transition. Cancer Lett. 2021;510:93-104. doi:10.1016/j.canlet.2021.04.007(IF:8.679)
[4] Dou R, Qian J, Wu W, et al. Suppression of steroid 5α-reductase type I promotes cellular apoptosis and autophagy via PI3K/Akt/mTOR pathway in multiple myeloma. Cell Death Dis. 2021;12(2):206. Published 2021 Feb 24. doi:10.1038/s41419-021-03510-4(IF:8.469)
[5] Hu W, Wang Z, Zhang H, et al. Chk1 Inhibition Ameliorates Alzheimer's Disease Pathogenesis and Cognitive Dysfunction Through CIP2A/PP2A Signaling. Neurotherapeutics. 2022;19(2):570-591. doi:10.1007/s13311-022-01204-z(IF:7.620)
[6] Sun J, Guo Y, Fan Y, Wang Q, Zhang Q, Lai D. Decreased expression of IDH1 by chronic unpredictable stress suppresses proliferation and accelerates senescence of granulosa cells through ROS activated MAPK signaling pathways. Free Radic Biol Med. 2021;169:122-136. doi:10.1016/j.freeradbiomed.2021.04.016(IF:7.376)
[7] Liu YJ, Xu WH, Fan LM, et al. Polydatin alleviates DSS- and TNBS-induced colitis by suppressing Th17 cell differentiation via directly inhibiting STAT3 [published online ahead of print, 2022 Jun 29]. Phytother Res. 2022;10.1002/ptr.7533. doi:10.1002/ptr.7533(IF:6.388)
[8] Zhang X, Cai Z, Wu M, Huangfu X, Li J, Liu X. Adipose Stem Cell-Derived Exosomes Recover Impaired Matrix Metabolism of Torn Human Rotator Cuff Tendons by Maintaining Tissue Homeostasis. Am J Sports Med. 2021;49(4):899-908. doi:10.1177/0363546521992469(IF:6.203)
[9] Gao C, Wang SW, Lu JC, et al. KSR2-14-3-3ζ complex serves as a biomarker and potential therapeutic target in sorafenib-resistant hepatocellular carcinoma. Biomark Res. 2022;10(1):25. Published 2022 Apr 25. doi:10.1186/s40364-022-00361-9(IF:6.148)
[10] Xu C, Zhang M, Bian L, Li Y, Yao Y, Li D. N-glycosylated SGK196 suppresses the metastasis of basal-like breast cancer cells. Oncogenesis. 2020;9(1):4. Published 2020 Jan 8. doi:10.1038/s41389-019-0188-1(IF:6.119)
[11] Wei H, Zhang HL, Wang XC, et al. Direct Activation of Protein Phosphatase 2A (PP2A) by Tricyclic Sulfonamides Ameliorates Alzheimer's Disease Pathogenesis in Cell and Animal Models. Neurotherapeutics. 2020;17(3):1087-1103. doi:10.1007/s13311-020-00841-6(IF:6.035)
[12] Li Z, Fu Y, Shen J, Liang J. Upstream Open Reading Frame Mediated Translation of WNK8 Is Required for ABA Response in Arabidopsis. Int J Mol Sci. 2021;22(19):10683. Published 2021 Oct 1. doi:10.3390/ijms221910683(IF:5.924)
[13] Zhou M, Zhang J, Zhou H, et al. Hydrogen Sulfide-Linked Persulfidation Maintains Protein Stability of ABSCISIC ACID-INSENSITIVE 4 and Delays Seed Germination. Int J Mol Sci. 2022;23(3):1389. Published 2022 Jan 26. doi:10.3390/ijms23031389(IF:5.924)
[14] Chen L, Xu W, Yang Q, et al. Imperatorin alleviates cancer cachexia and prevents muscle wasting via directly inhibiting STAT3. Pharmacol Res. 2020;158:104871. doi:10.1016/j.phrs.2020.104871(IF:5.893)
[15] Li F, Hu X, Wu J. Daidzein Activates Akt Pathway to Promote the Proliferation of Female Germline Stem Cells through Upregulating Clec11a [published online ahead of print, 2022 Jun 3]. Stem Cell Rev Rep. 2022;10.1007/s12015-022-10394-0. doi:10.1007/s12015-022-10394-0(IF:5.739)
[16] Liu X, Sun C, Zou K, et al. Novel PGK1 determines SKP2-dependent AR stability and reprograms granular cell glucose metabolism facilitating ovulation dysfunction. EBioMedicine. 2020;61:103058. doi:10.1016/j.ebiom.2020.103058(IF:5.736)
[17] Li PP, Li RG, Huang YQ, Lu JP, Zhang WJ, Wang ZY. LncRNA OTUD6B-AS1 promotes paclitaxel resistance in triple negative breast cancer by regulation of miR-26a-5p/MTDH pathway-mediated autophagy and genomic instability. Aging (Albany NY). 2021;13(21):24171-24191. doi:10.18632/aging.203672(IF:5.682)
[18] Bian C, Duan Y, Xiu Q, Wang J, Tao X, Zhou M. Mechanism of validamycin A inhibiting DON biosynthesis and synergizing with DMI fungicides against Fusarium graminearum. Mol Plant Pathol. 2021;22(7):769-785. doi:10.1111/mpp.13060(IF:5.663)
[19] Wu L, Yuan Z, Wang P, Mao X, Zhou M, Hou Y. The plasma membrane H⁺ -ATPase FgPMA1 regulates the development, pathogenicity, and phenamacril sensitivity of Fusarium graminearum by interacting with FgMyo-5 and FgBmh2. Mol Plant Pathol. 2022;23(4):489-502. doi:10.1111/mpp.13173(IF:5.663)
[20] Dou B, Zhou W, Li S, et al. Buyang Huanwu Decoction Attenuates Infiltration of Natural Killer Cells and Protects Against Ischemic Brain Injury. Cell Physiol Biochem. 2018;50(4):1286-1300. doi:10.1159/000494587(IF:5.500)
[21] Fang DD, Tang Q, Kong Y, et al. MDM2 inhibitor APG-115 exerts potent antitumor activity and synergizes with standard-of-care agents in preclinical acute myeloid leukemia models. Cell Death Discov. 2021;7(1):90. Published 2021 May 3. doi:10.1038/s41420-021-00465-5(IF:5.241)
[22] Zhao YH, Li H, Zhao H, Sun WK, Wang Q, Li WW. An ancient interleukin-16-like molecule regulates hemocyte proliferation via integrin β1 in invertebrates. J Biol Chem. 2021;297(2):100943. doi:10.1016/j.jbc.2021.100943(IF:5.157)
[23] Li J, Yao Z, Xiong H, et al. Extracellular vesicles from hydroxycamptothecin primed umbilical cord stem cells enhance anti-adhesion potential for treatment of tendon injury. Stem Cell Res Ther. 2020;11(1):500. Published 2020 Nov 25. doi:10.1186/s13287-020-02016-8(IF:5.116)
[24] Liu H, Zhu H, Xia H, et al. Different effects of high-fat diets rich in different oils on lipids metabolism, oxidative stress and gut microbiota. Food Res Int. 2021;141:110078. doi:10.1016/j.foodres.2020.110078(IF:4.972)
[25] Zheng Y, Luo A, Liu X. The Imbalance of Mitochondrial Fusion/Fission Drives High-Glucose-Induced Vascular Injury. Biomolecules. 2021;11(12):1779. Published 2021 Nov 27. doi:10.3390/biom11121779(IF:4.879)
[26] Wang X, Liu H, Liu Y, et al. Highly Conserved C-Terminal Region of Indian Hedgehog N-Fragment Contributes to Its Auto-Processing and Multimer Formation. Biomolecules. 2021;11(6):792. Published 2021 May 25. doi:10.3390/biom11060792(IF:4.879)
[27] Wang S, Hao Q, Li J, et al. Ubiquitin ligase DTX3 empowers mutant p53 to promote ovarian cancer development. Genes Dis. 2020;9(3):705-716. Published 2020 Nov 21. doi:10.1016/j.gendis.2020.11.007(IF:4.803)
[28] Wang Y, Yin Y, Chen X, et al. Induction of Intestinal Th17 Cells by Flagellins From Segmented Filamentous Bacteria. Front Immunol. 2019;10:2750. Published 2019 Nov 22. doi:10.3389/fimmu.2019.02750(IF:4.716)
[29] Zheng J, Lou L, Fan J, et al. Commensal Escherichia coli Aggravates Acute Necrotizing Pancreatitis through Targeting of Intestinal Epithelial Cells. Appl Environ Microbiol. 2019;85(12):e00059-19. Published 2019 May 30. doi:10.1128/AEM.00059-19(IF:4.077)
[30] Zhang QC, Zou YP, Hu SQ, et al. TNF-α-stimulated nucleus pulposus cells induce cell apoptosis through the release of exosomal miR-16 targeting IGF-1 and IGF-1R in rats. Ann Transl Med. 2021;9(17):1376. doi:10.21037/atm-21-227(IF:3.932)
[31] Lu T, Zhao WE, Zhang F, Qi X, Yang Y, Gu C. Lycium barbarum polysaccharides attenuate rat anti-Thy-1 glomerulonephritis through mediating pyruvate dehydrogenase. Biomed Pharmacother. 2019;116:109020. doi:10.1016/j.biopha.2019.109020(IF:3.743)
[32] Lin Z, Wang J, Zhao S, et al. Goose IRF7 is involved in antivirus innate immunity by mediating IFN activation. Dev Comp Immunol. 2022;133:104435. doi:10.1016/j.dci.2022.104435(IF:3.636)
[33] Chen Y, Cao A, Li Q, Quan J. Identification of DNA aptamers that specifically targets EBV⁺ nasopharyngeal carcinoma via binding with EphA2/CD98hc complex. Biochem Biophys Res Commun. 2022;608:135-141. doi:10.1016/j.bbrc.2022.03.157(IF:3.575)
[34] Sun H, Yao W, Wang K, Qian Y, Chen H, Jung YS. Inhibition of neddylation pathway represses influenza virus replication and pro-inflammatory responses. Virology. 2018;514:230-239. doi:10.1016/j.virol.2017.11.004(IF:3.353)
[35] Hu X, Zhang J, Bu J, et al. MiR-4733-5p promotes gallbladder carcinoma progression via directly targeting kruppel like factor 7. Bioengineered. 2022;13(4):10691-10706. doi:10.1080/21655979.2022.2065951(IF:3.269)
[36] Li W, Li Y, Gan L, Ma F, Zhang S, Wen T. Dcf1 alleviates C99-mediated deficits in drosophila by reducing the cleavage of C99. Biochem Biophys Res Commun. 2020;530(2):410-417. doi:10.1016/j.bbrc.2020.05.063(IF:2.985)
[37] Shen J, Li Z, Fu Y, Liang J. Identification and molecular characterization of the alternative spliced variants of beta carbonic anhydrase 1 (βCA1) from Arabidopsis thaliana. PeerJ. 2021;9:e12673. Published 2021 Dec 23. doi:10.7717/peerj.12673(IF:2.984)
[38] Zhang QC, Hu SQ, Hu AN, Zhang TW, Jiang LB, Li XL. Autophagy-activated nucleus pulposus cells deliver exosomal miR-27a to prevent extracellular matrix degradation by targeting MMP-13. J Orthop Res. 2021;39(9):1921-1932. doi:10.1002/jor.24880(IF:2.728)
[39] Zhou K, Sun M, Xia Y, Xie Y, Shu R. LPS stimulates gingival fibroblasts to express PD-L1 via the p38 pathway under periodontal inflammatory conditions. Arch Oral Biol. 2021;129:105161. doi:10.1016/j.archoralbio.2021.105161(IF:2.635)
[40] Zhu N, Hu K, Li Z, Chen Y, Liu Y. Micronuclei Formation by Promutagens in Metabolism-Incompetent V79 Cells Interacting With Activation-Proficient Cells in Various Experimental Settings. Environ Mol Mutagen. 2020;61(2):224-234. doi:10.1002/em.22309(IF:2.528)
[41] Zhan J, Hu P, Wang Y. lncRNA PVT1 aggravates doxorubicin-induced cardiomyocyte apoptosis by targeting the miR-187-3p/AGO1 axis. Mol Cell Probes. 2020;49:101490. doi:10.1016/j.mcp.2019.101490(IF:2.511)
[42] Peng H, Luo Y, Ying Y. lncRNA XIST attenuates hypoxia-induced H9c2 cardiomyocyte injury by targeting the miR-122-5p/FOXP2 axis. Mol Cell Probes. 2020;50:101500. doi:10.1016/j.mcp.2019.101500(IF:2.511)
[43] Huang Y, Liang H, He C, Peng F. Hepatitis B Virus X Protein-Induced RORγ Expression to Promote the Migration and Proliferation of Hepatocellular Carcinoma. Biomed Res Int. 2019;2019:5407126. Published 2019 Nov 3. doi:10.1155/2019/5407126(IF:2.197)
[44] Fang J, Zheng W, Hu P, Wu J. Investigating the effect of lncRNA HOTAIR on apoptosis induced by myocardial ischemia-reperfusion injury. Mol Med Rep. 2021;23(3):169. doi:10.3892/mmr.2020.11808(IF:2.100)
[45] Huangfu S, Dou R, Zhong S, et al. Modified Pulsatillae decoction inhibits DSS-induced ulcerative colitis in vitro and in vivo via IL-6/STAT3 pathway. BMC Complement Med Ther. 2020;20(1):179. Published 2020 Jun 9. doi:10.1186/s12906-020-02974-9(IF:0.000)