AddexBio总 代理


AddexBio总 代理

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上海金畔生物科技有限公司AddexBio专业代理,具体产品信息欢迎电询:021-50837765

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“AddexBio细胞株SJSA-1cells(骨肉瘤;多潜能肉瘤)”

疾病: 骨肉瘤;多潜能肉瘤 

来源: 原发性股骨的原始多向肉瘤

种类: 人

组织: 骨头

Disease:

Osteosarcoma; multipotential sarcoma 

Origin:

Derived from primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur 

Species:

Homo sapiens 

Tissue:

Bone 

Morphology:

Fibroblast 

Properties:

Adherent 

Cytogenic data:

SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene 

Patient:

Male, Black, 19 years of age 

Medium:

RPMI1640 + 10% FBS 

Subculture:

1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

 I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Isoenzymes:

 Not known 

DNA Profile (STR):

 Not known 

Mutations in SJSA-1 Cells 

References for SJSA-1 cells:

1. Bransfield, K. L., Askham, J. M., Leek, J. P., Robinson, P. A., and Mighell, A. J. Phenotypic changes associated with DYNACTIN-2 (DCTN2) over expression characterise SJSA-1 osteosarcoma cells. Mol Carcinog, 45: 157-163, 2006. 

2. Missirlis, D., Khant, H., and Tirrell, M. Mechanisms of peptide amphiphile internalization by SJSA-1 cells in vitro. Biochemistry, 48: 3304-3314, 2009. 

3. Missirlis, D., Krogstad, D. V., and Tirrell, M. Internalization of p53(14-29) peptide amphiphiles and subsequent endosomal disruption results in SJSA-1 cell death. Mol Pharm, 7: 2173-2184.

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