标签归档：hrp

Jackson ImmunoResearch抗HRP抗体

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Jackson ImmunoResearch抗HRP抗体

简要描述：美国Jackson ImmunoResearch Laboratories, Inc. 专业致力于亲合纯化的第二抗体和纯化的 免疫球蛋白的生产和偶联。

详细介绍

产品咨询

美国Jackson ImmunoResearch Laboratories, Inc. 专业致力于亲合纯化的第二抗体和纯化的 免疫球蛋白的生产和偶联。其产品深受广大研究人员的认可，销售范围遍布， 领域包括植物，动物和生物医学研究。公司成立于1982年，公司创业者具有深厚的 专业背景：细胞生物学，蛋白化学，微生物学，免疫学。公司自创立初，就将为其 他研究人员提供zui全的二抗作为公司的持续发展目标。

Probe Host Code Number Size Price
  Unconjugated Goat
Rabbit		 123-005-021
323-005-021		 2.0 mg
2.0 mg		 $ 60
75
  Aminomethylcoumarin, AMCA      （A=350, E=450） Goat
Rabbit		 123-155-021
323-155-021		 2.0 mg
1.5 mg		 79
79
  Cy2, Cyanine      （A=492, E=510） Goat 123-225-021
 		 2.0 mg
 		 87
  DyLight 488      （A=493, E=518） Rabbit 323-485-021 1.0 mg 91
  Alexa Fluor® 488   （A=493, E=519）                     NEW Goat
Rabbit		 123-545-021
323-545-021		 1.5 mg
1.0 mg		 91
91
  Fluorescein, FITC     （A=492, E=520） Goat
Rabbit		 123-095-021
323-095-021		 2.0 mg
1.5 mg		 68
68
  DyLight 549     （A=555, E=568） Rabbit 323-505-021 1.5 mg 91
  Cy3, Indocarbocyanine     （A=550, E=570） Goat
Rabbit		 123-165-021
323-165-021		 2.0 mg
1.5 mg		 87
87
  Tetramethyl Rhodamine, TRITC     （A=550, E=570） Goat
Rabbit		 123-025-021
323-025-021		 2.0 mg
1.5 mg		 68
68
  Rhodamine Red-X, RRX     （A=570, E=590） Goat
Rabbit		 123-295-021
323-295-021		 2.0 mg
1.5 mg		 68
68
  Alexa Fluor® 594  （A=591, E=614）                                     NEW Goat
Rabbit		 123-585-021
323-585-021		 1.5 mg
1.0 mg		 91
91
  DyLight 594     （A=591, E=616） Goat
Rabbit		 123-515-021
323-515-021		 2.0 mg
1.5 mg		 91
91
  Alexa Fluor® 647  （A=651, E=667）                                      NEW Goat
Rabbit		 123-605-021
323-605-021		 1.5 mg
1.0 mg		 91
91
  DyLight 649     （A=652, E=670） Rabbit 323-495-021 1.5 mg 91
  Biotin-SP （long-spacer） Goat
Rabbit		 123-065-021
323-065-021		 2.0 ml
1.5 ml		 102
102
  Alkaline Phosphatase Goat
Rabbit		 123-055-021
323-055-021		 1.0 ml
1.0 ml		 114
118
  4 nm Colloidal Gold （LM） Goat 123-185-021 1.0 ml 112
  6 nm Colloidal Gold （EM） Goat 123-195-021 0.5 ml 98
  12 nm Colloidal Gold （EM） Goat 123-205-021 0.5 ml 70
  18 nm Colloidal Gold （EM） Goat 123-215-021 0.5 ml 98

HRP/AP酶稳定剂 蛋白 / 抗体稳定剂–

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HRP/AP酶稳定剂  蛋白 / 抗体稳定剂–Jinpan


金畔生物稳定剂可根据配方设计者的设计目的,可以灵活的使用稳定剂以达到产品品质稳定的目的，相关货号如下：

货号 品名 规格
78BC10013 HRP 稳定剂 100mL 20L 1L
78BC10014 无蛋白HRP稳定剂 100mL 20L 1L
78BC10015 HRP稳定剂 PIus 100mL 20L 1L
78BC10016 抗体稳定剂 TRIS 100mL 20L 1L
78BC10017 抗体稳定剂 PBS 100mL 20L 1L
78BC10018 板稳定剂 100mL 20L 1L
78BC10019 无蛋白板稳定剂 100mL 20L 1L
78BC10020 AP稳定剂 100mL 20L 1L
78BC10021 AP稳定剂 TRIS 100mL 20L 1L
78BC10022 包被微球稳定剂(PBS,无蛋白) 100mL 20L 1L
78BC10023 包被微球稳定剂 TRIS 100mL 20L 1L

Proteintech HRP-66005 现货

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世界*实验材料供应商 Proteintech Group，Inc.上海金畔生物为其中国代理， Proteintech Group，Inc.在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， Proteintech Group，Inc.就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

 Proteintech Group，Inc.中国代理, Proteintech Group，Inc.上海代理， Proteintech Group，Inc.北京代理，Proteintech Group，Inc.广东代理， Proteintech Group，Inc.江苏代理Proteintech Group，Inc.湖北代理,Proteintech Group，Inc.天津,Proteintech Group，Inc.黑龙江代理,Proteintech Group，Inc.内蒙古代理,Proteintech Group，Inc.吉林代理,Proteintech Group，Inc.福建代理, Proteintech Group，Inc.江苏代理,Proteintech Group，Inc.浙江代理, Proteintech Group，Inc.四川代理,

 

Proteintech Group，Inc.于2001年在美国芝加哥成立，是一家专门从事研发、生产和销售抗体及重组蛋白的生物公司。针对生物前沿的热点研究，为科研工作者提 供新颖*的一抗及相关产品。武汉三鹰生物技术有限公司是Proteintech Group，Inc.在中国成立的全资子公司。

公司自创建以来，一贯以感受现代生物学发展的脉搏，把握当今生物学先进的技术，为生物学研究提供所需的抗 体、蛋白及相关产品，为生物学界同仁提供优良服务为己任。公司拥有*管理体系，确立了一整套生物科技企业的良性运营模式，建立起了坚实而广泛的欧美 以及国内市场销售体系，产品和服务已为国内外*实验室所接纳和采用，在当今方兴未艾的人类蛋白质组计划已占据了一席之地。

Proteintech建立了拥有22，000条人类基因的cDNA文库，10，000多种人类蛋白质抗体库。公司以强大的研发和技术能力，面向国内外所 有的生物实验室提供优良的技术服务，以及从克隆到大规模表达和抗体制备的整套。

 

HRP-Conjugated 6*His, His-Tag Antibody

 

货号: HRP-66005

小鼠单克隆

将重组蛋白进行SDS PAGE，随后用HRP-66005（6 * His，His-Tag抗体）以1:100稀释的蛋白质印迹

物种特异性：  重组蛋白，人

正面WB检测：  重组蛋白

推荐稀释度   WB：1：5000-1：50000

 

产品信息

资源： 老鼠

净化方法： 蛋白G纯化

同种型：   IgG1

存储：    PBS含0.02％硫柳汞，50％甘油，pH 7.3。储存于-20 Ô C.等分是不必要的-20 ö下储         存。

 

免疫原信息

免疫原：  6xHis肽

全名：   6 *他，他的标签

计算分子量：  1 kDa

观察分子量：  –

GenBank登录号：

基因ID（NCBI）： 

基因符号

同义词   6 * His，6 * His，His Tag，6histag，6XHis，HHHHHH，His tag，HISTIDINE TAGGED，HRP 6XHis，HRP HHHHHH，HRP His tag

 

货号 产品名称 规格型号 品牌
HRP-66005 HRP-conjugated 6*His, His-Tag Antibody 100ul Proteintech

Proteintech HRP-66005 HRP-conjugated 6*His, His-Tag Antibody 现货供应

 

 

kementec ELISA HRP 底物说明书

发表于由

 

kementec ELISA HRP 底物说明书

TMB PLUS2®（和预染）

ELISA HRP 底物

编号 4395 / 4445

TMB (3, 3', 5, 5'-tetramethylbenzidine) 是辣根过氧化物酶 (HRP) 的显色底物。在 HRP 对过氧化氢的酶促降解过程中，TMB 会产生深蓝色，添加终止溶液会产生在 450 nm 处吸收的透明黄色。与 OPD（邻苯二胺二氯化物）相比，该底物在 ELISA 中具有更高的信号和更高的灵敏度。

TMB PLUS2® 的特点是：

  • 中等灵敏度

  • 背景非常低

  • 水基 – 不含有机溶剂（符合 REACH 附件 XVII）

  • 批次间差异小

对于可追溯的移液，我们建议使用粉色 TMB PLUS2® Prestained（货号 4445）。加入终止溶液后，粉红色指示剂消失。

三个不同尺寸的棕色实验室瓶，带有 Kementec Solutions TMB PLUS2

 

 

 

现货affinitybiologicals SAF8C-HRP说明书

发表于由


affinitybiologicals SAF8C-HRP说明书


因子 VIII:C 多克隆抗体 – HRP 偶联

 

SAF8C-HRP – 绵羊抗人因子 VIII:C，过氧化物酶偶联 IgG（0.2 毫克小瓶）

类别：抗体,因子 VIII 抗体,因子 VIII 产品

因子 VIII:C 多克隆抗体 – HRP 偶联

Affinity 的因子 VIII:C 多克隆抗体 – HRP 偶联是我们辣根过氧化物酶偶联因子 VIII:C 抗体的基础水平。IgG 的纯度通常为 90%，以含有 50% 甘油 (v/v) 的 HEPES 缓冲盐水溶液形式提供。滴度与起始抗血清基本相同，每个小瓶通常含有从一毫升抗血清中回收的 IgG 量，但该 IgG 已与辣根过氧化物酶结合作为酶报告基因。这种因子 VIII:C 多克隆抗体 – HRP 偶联物通常用作标记的一抗，用于免疫分析和免疫印迹等应用。

产品编号： SAF8C-HRP

零售产品尺寸： 0.2mg 小瓶

宿主动物：绵羊抗人因子 VIII:C 多克隆抗体 – HRP 偶联

因子 VIII 的描述

因子 VIII（以前称为抗血友病球蛋白和因子 VIII:C）是一种大糖蛋白 (320 kDa)，以大约 200 ng/ml 的浓度在血浆中循环。在肝脏中合成，大部分因子 VIII 在表达过程中被切割，导致部分切割形式的 FVIII 的异质混合物，大小范围为 200-280 kDa。FVIII 通过与血管性血友病因子结合形成 FVIII 体内正常存活所需的 FVIII-vWF 复合物而稳定（t1/2 为 8-12 小时）。

F.VIII 是一种前辅因子，通过凝血酶有限的蛋白水解作用被激活。在这个过程中，F.VIIIa 从 vWF 解离，与活化的因子 IX、钙和磷脂表面结合，在那里它是因子 X 激活剂复合物组装中不可少的辅助因子。一旦与 vWF 分离，FVIIIa 很容易被活化的蛋白 C 和非酶促衰变灭活。

血友病 A 是一种先天性出血性疾病，由 X 染色体相关的 FVIII 缺乏引起。缺乏的严重程度通常与疾病的严重程度相关。一些血友病患者 (~10%) 会产生部分或*失活的 FVIII 蛋白。5-20% 的血友病患者也会产生针对 FVIII 的中和抗体1-3。

 

过氧化物酶HRP标记山羊抗兔IgG抗体|Peroxidase-Conjugated Goat Anti-Rabbit IgG

发表于由

过氧化物酶HRP标记山羊抗兔IgG抗体|Peroxidase-Conjugated Goat Anti-Rabbit IgG

产品说明书

FAQ

COA

已发表文献

产品简介

本品是由辣根过氧化酶（HRP）标记的山羊抗兔IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。

本品适合做单标实验。要做多标（Multiple-labeling）实验，建议使用经过与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

 

产品应用

建议稀释浓度：

IHC/ICC：1:500-1:5000；

ELISA/WB(显色法)：1:5000-1:100000；

ELISA/WB(化学发光法)：1:10000-1:200000；

 

产品信息

抗体浓度（Antibody Concentration）

100 μL（0.4 mg/mL）

缓冲液（Buffer）

0.005M 磷酸钠，0.125M 氯化钠，pH7.6

稳定剂（Stabilizer）

7.5 mg/mL BSA（无IgG，蛋白酶)， 50%甘油

防腐剂（Preservative）

None（叠氮化钠会抑制HRP活性）

原料来源（Source of Material）

Jackson Immunoresearch 111-035-003

 

储存条件

-25 ~ -15℃分装保存，尽量避免反复冻融。有效期1年。

 

注意事项

1）本品不能保存于叠氮化钠中其会抑制辣根过氧化酶（HRP）活性；

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

3）本产品仅作科研用途！

 

过氧化物酶HRP标记山羊抗兔IgG抗体|Peroxidase-Conjugated Goat Anti-Rabbit IgG

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[33] Chen Z, Li Z, Jiang C, Jiang X, Zhang J. MiR-92b-3p promotes neurite growth and functional recovery via the PTEN/AKT pathway in acute spinal cord injury. J Cell Physiol. 2019;234(12):23043-23052. doi:10.1002/jcp.28864(IF:4.522)
[34] Yao H, Xu K, Zhou J, Zhou L, Wei S. A Tumor Microenvironment Destroyer for Efficient Cancer Suppression. ACS Biomater Sci Eng. 2020;6(1):450-462. doi:10.1021/acsbiomaterials.9b01544(IF:4.511)
[35] Sun X, Zhang J, Nie Q. Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples. PLoS Comput Biol. 2021;17(3):e1008379. Published 2021 Mar 5. doi:10.1371/journal.pcbi.1008379(IF:4.475)
[36] Yin L, Zhang L, Luo L, et al. Berbamine reduces body weight via suppression of small GTPase Rab8a activity and activation of paraventricular hypothalamic neurons in obese mice. Eur J Pharmacol. 2022;916:174679. doi:10.1016/j.ejphar.2021.174679(IF:4.432)
[37] Fang DD, Zhu H, Tang Q, et al. FLT3 inhibition by olverembatinib (HQP1351) downregulates MCL-1 and synergizes with BCL-2 inhibitor lisaftoclax (APG-2575) in preclinical models of FLT3-ITD mutant acute myeloid leukemia. Transl Oncol. 2022;15(1):101244. doi:10.1016/j.tranon.2021.101244(IF:4.243)
[38] Li D, Qiu X, Yang J, Liu T, Luo Y, Lu Y. Generation of Human Lens Epithelial-Like Cells From Patient-Specific Induced Pluripotent Stem Cells. J Cell Physiol. 2016;231(12):2555-2562. doi:10.1002/jcp.25374(IF:4.155)
[39] Su J, Shen Y, Li N, et al. Comparative Characterization of CpCDPK1 and CpCDPK9, Two Potential Drug Targets Against Cryptosporidiosis. Microorganisms. 2022;10(2):333. Published 2022 Feb 1. doi:10.3390/microorganisms10020333(IF:4.128)
[40] Guo Y, Huo J, Wu D, et al. Simvastatin inhibits the adipogenesis of bone marrow‑derived mesenchymal stem cells through the downregulation of chemerin/CMKLR1 signaling. Int J Mol Med. 2020;46(2):751-761. doi:10.3892/ijmm.2020.4606(IF:4.101)
[41] Yang B, Li Y, Ma Y, et al. Selenium attenuates ischemia/reperfusion injury‑induced damage to the blood‑brain barrier in hyperglycemia through PI3K/AKT/mTOR pathway‑mediated autophagy inhibition. Int J Mol Med. 2021;48(3):178. doi:10.3892/ijmm.2021.5011(IF:4.101)
[42] Liu S, Xu DS, Ma JL, Huang P, Wu D, Ren LQ. LncRNA H19 Mitigates Oxidized Low-Density Lipoprotein Induced Pyroptosis via Caspase-1 in Raw 264.7 Cells. Inflammation. 2021;44(6):2407-2418. doi:10.1007/s10753-021-01511-1(IF:4.092)
[43] Zhang H, Guo F, Qi P, et al. OsHDA710-Mediated Histone Deacetylation Regulates Callus Formation of Rice Mature Embryo. Plant Cell Physiol. 2020;61(9):1646-1660. doi:10.1093/pcp/pcaa086(IF:4.062)
[44] Sun T, Zhang L, Feng J, et al. Characterization of cellular senescence in doxorubicin-induced aging mice. Exp Gerontol. 2022;163:111800. doi:10.1016/j.exger.2022.111800(IF:4.032)
[45] Wang J, Zhang Y, Liu X, Liu H. Is the Fixed Periodic Treatment Effective for the Tumor System without Complete Information?. Cancer Manag Res. 2021;13:8915-8928. Published 2021 Nov 30. doi:10.2147/CMAR.S339787(IF:3.989)
[46] Chen M, Lin H, Gao Y, Wang Z, Li Y, Jin F. Ghrelin attenuates drowning injury via dual effects on damage protection and immune repression. Ann Transl Med. 2021;9(11):920. doi:10.21037/atm-21-795(IF:3.932)
[47] Shi L, Zhang XB, Shi YF, et al. OsCDC48/48E complex is required for plant survival in rice (Oryza sativa L.). Plant Mol Biol. 2019;100(1-2):163-179. doi:10.1007/s11103-019-00851-9(IF:3.928)
[48] Shi J, Wang Y, Chen J, et al. Synthesis and biological evaluation of 1,2,4-oxadiazole core derivatives as potential neuroprotectants against acute ischemic stroke. Neurochem Int. 2021;148:105103. doi:10.1016/j.neuint.2021.105103(IF:3.921)
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产品简介

本品是由辣根过氧化酶（HRP）标记的山羊抗兔IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。

本品适合做单标实验。要做多标（Multiple-labeling）实验，建议使用经过与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

 

产品应用

建议稀释浓度：

IHC/ICC：1:500-1:5000；

ELISA/WB(显色法)：1:5000-1:100000；

ELISA/WB(化学发光法)：1:10000-1:200000；

 

产品信息

抗体浓度（Antibody Concentration）

100 μL（0.4 mg/mL）

缓冲液（Buffer）

0.005M 磷酸钠，0.125M 氯化钠，pH7.6

稳定剂（Stabilizer）

7.5 mg/mL BSA（无IgG，蛋白酶)， 50%甘油

防腐剂（Preservative）

None（叠氮化钠会抑制HRP活性）

原料来源（Source of Material）

Jackson Immunoresearch 111-035-003

 

储存条件

-25 ~ -15℃分装保存，尽量避免反复冻融。有效期1年。

 

注意事项

1）本品不能保存于叠氮化钠中其会抑制辣根过氧化酶（HRP）活性；

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

3）本产品仅作科研用途！

 

过氧化物酶HRP标记山羊抗兔IgG抗体|Peroxidase-Conjugated Goat Anti-Rabbit IgG

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[49] Zhang KW, Wang D, Cai H, et al. IL‑6 plays a crucial role in epithelial‑mesenchymal transition and pro‑metastasis induced by sorafenib in liver cancer. Oncol Rep. 2021;45(3):1105-1117. doi:10.3892/or.2021.7926(IF:3.906)
[50] Qu F, Song Y, Wu Y, et al. The protective role of Ephrin-B2/EphB4 signaling in osteogenic differentiation under inflammatory environment. Exp Cell Res. 2021;400(2):112505. doi:10.1016/j.yexcr.2021.112505(IF:3.905)
[51] Cheng Q, Fan X, Liu Y, et al. miR-455-5p regulates circadian rhythms by accelerating the degradation of Clock mRNA. IUBMB Life. 2022;74(3):245-258. doi:10.1002/iub.2587(IF:3.885)
[52] He RZ, Zheng JH, Yao HF, et al. ADAMTS12 promotes migration and epithelial-mesenchymal transition and predicts poor prognosis for pancreatic cancer [published online ahead of print, 2022 Apr 25]. Hepatobiliary Pancreat Dis Int. 2022;S1499-3872(22)00117-5. doi:10.1016/j.hbpd.2022.04.005(IF:3.780)
[53] Wang Y, Wang H, Zhang L, et al. Potential mechanisms of tremor tolerance induced in rats by the repeated administration of total alkaloid extracts from the seeds of Peganum harmala Linn. J Ethnopharmacol. 2020;262:113183. doi:10.1016/j.jep.2020.113183(IF:3.690)
[54] Bu S, Li B, Wang Q, et al. Epithelial ovarian cancer stem‑like cells are resistant to the cellular lysis of cytokine‑induced killer cells via HIF1A‑mediated downregulation of ICAM‑1. Int J Oncol. 2019;55(1):179-190. doi:10.3892/ijo.2019.4794(IF:3.571)
[55] Shen Y, Zhou M, Yan J, et al. miR-200b inhibits TNF-α-induced IL-8 secretion and tight junction disruption of intestinal epithelial cells in vitro. Am J Physiol Gastrointest Liver Physiol. 2017;312(2):G123-G132. doi:10.1152/ajpgi.00316.2016(IF:3.468)
[56] Yao C, Chen X, Xu Y, et al. Comparing pretreatment strategies to increase the yield and purity of human urinary extracellular vesicles [published online ahead of print, 2022 Jun 30]. J Chromatogr B Analyt Technol Biomed Life Sci. 2022;1206:123359. doi:10.1016/j.jchromb.2022.123359(IF:3.318)
[57] Cao H, Hua D, Zhang H, et al. Oral immunization of recombinant Saccharomyces cerevisiae expressing fiber-2 of fowl adenovirus serotype 4 induces protective immunity against homologous infection. Vet Microbiol. 2022;271:109490. doi:10.1016/j.vetmic.2022.109490(IF:3.293)
[58] Li Q , Sun J , Mohammadtursun N , Wu J , Dong J , Li L . Curcumin inhibits cigarette smoke-induced inflammation via modulating the PPARγ-NF-κB signaling pathway. Food Funct. 2019;10(12):7983-7994. doi:10.1039/c9fo02159k(IF:3.241)
[59] Yang LY, Liu XF, Yang Y, et al. Biochemical features of the adhesion G protein-coupled receptor CD97 related to its auto-proteolysis and HeLa cell attachment activities. Acta Pharmacol Sin. 2017;38(1):56-68. doi:10.1038/aps.2016.89(IF:3.166)
[60] Fei J, Wu H, Su J, et al. Characterization of MEDLE-1, a protein in early development of Cryptosporidium parvum. Parasit Vectors. 2018;11(1):312. Published 2018 May 23. doi:10.1186/s13071-018-2889-2(IF:3.163)
[61] Yao Y, Luo R, Xiong S, Zhang C, Zhang Y. Protective effects of curcumin against rat intestinal inflammation‑related motility disorders. Mol Med Rep. 2021;23(5):391. doi:10.3892/mmr.2021.12030(IF:2.952)
[62] Li X, An P, Zhao Y, et al. Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells. Evid Based Complement Alternat Med. 2022;2022:3664696. Published 2022 Apr 22. doi:10.1155/2022/3664696(IF:2.630)
[63] Li D, Han X, Zhao Z, Lu Y, Yang J. Functional analysis of deleterious EPHA2 SNPs in lens epithelial cells. Mol Vis. 2021;27:384-395. Published 2021 Jun 23. (IF:2.367)
[64] You J, Cheng Y, Yang XJ, Chen L. Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system. Stem Cell Res. 2021;53:102342. doi:10.1016/j.scr.2021.102342(IF:2.020)
[65] You J, Xi H, Ma S, Yang XJ, Chen L. Generation of a homozygous LRPAP1 knockout human embryonic stem cell line (FDCHDPe009-B) by CRISPR/Cas9 system. Stem Cell Res. 2021;56:102516. doi:10.1016/j.scr.2021.102516(IF:2.020)
[66] Zhu T, Zhao Y, Wu Y, Qu D. The Staphylococcus epidermidis gdpS regulates biofilm formation independently of its protein-coding function. Microb Pathog. 2017;105:264-271. doi:10.1016/j.micpath.2017.02.045(IF:2.009)
[67] Liu X, Xie F, Lai G, Wang J. Roles of heterogeneous nuclear ribonucleoprotein L in enamel organ development and the differentiation of ameloblasts. Arch Oral Biol. 2020;120:104933. doi:10.1016/j.archoralbio.2020.104933(IF:1.931)
[68] Jiang J, Chen Z, Yang Y, Yan J, Jiang H. Sevoflurane downregulates IGF‑1 via microRNA‑98. Mol Med Rep. 2017;15(4):1863-1868. doi:10.3892/mmr.2017.6219(IF:1.692)
[69] Xia Q, Zhou Y, Wang X, Fu S. Interleukin-1 receptor-associated kinase 3 downregulation in peripheral blood mononuclear cells attenuates immunosuppression in sepsis. Exp Ther Med. 2018;15(2):1586-1593. doi:10.3892/etm.2017.5549(IF:1.261)
[70] Li X, Lei Y, Yu Y, et al. Discovery and characterization of a novel splice variant of the p53 tumor suppressor gene in a human T cell leukemia cellline. Int J Clin Exp Pathol. 2020;13(5):1121-1135. Published 2020 May 1. (IF:0.252)
[71] Feng Y, Jiang C, Yang F, Chen Z, Li Z. Apocynum venetum leaf extract protects against H2O2-induced oxidative stress by increasing autophagy in PC12 cells. Biomed Rep. 2020;13(2):6. doi:10.3892/br.2020.1313(IF:0.000)

HRP Streptavidin 辣根过氧化物酶标记链霉亲和素 HRP链霉亲和素

发表于由

HRP Streptavidin 辣根过氧化物酶标记链霉亲和素 HRP链霉亲和素

产品说明书

FAQ

COA

已发表文献

链霉亲和素是一种来源于阿维丁链霉菌（streptomyces avidinii）的生物素结合蛋白，其非特异性结合远比亲和素低，且与蛋清亲和素在中性pH值具有净正电荷和包含大约7%的碳水化合物相比，链霉亲和素蛋白具有在中性pH值几乎没有净电荷，不含有碳水化合物等更有利的化学性质，所以它被广泛用来作为抗生物素蛋白的替代品。

本品是辣根过氧化物酶标记的链霉亲和素，以液体形式提供，含50%甘油。

建议工作液浓度：0.01-0.1µg/ml for WB（化学发光法）；1-2µg/ml for ELISA,IHC/ICC,WB(底物显色法)。
 

产品性质 

链霉亲和素浓度（Streptavidin Concentration）

0.5 mg/ml

缓冲液（Buffer)

0.005M 磷酸钠，0.125M 氯化钠, pH 7.6

稳定剂（Stabilizer）

7.5mg/ml BSA（无IgG，蛋白酶）

防腐剂（Preservative）

None（叠氮化钠会抑制辣根过氧化物酶的活性）

运输与保存方法

冰袋运输。-20℃保存，有效期1年。避免反复冻融。

注意事项

1) 为了您的安全和健康，请穿实验服并戴一次性手套操作。
2）本产品仅作科研用途！

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HRP Streptavidin 辣根过氧化物酶标记链霉亲和素 HRP链霉亲和素

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[1] Wu H, Zeng L, Ou J, Wang T, Chen Y, Nandakumar KS. Estrogen Acts Through Estrogen Receptor-β to Promote Mannan-Induced Psoriasis-Like Skin Inflammation. Front Immunol. 2022;13:818173. Published 2022 May 19. doi:10.3389/fimmu.2022.818173(IF:7.561)
[2] Zhou Y, Xiong H, Chen R, et al. Aptamer Detection of Mycobaterium tuberculosis Mannose-Capped Lipoarabinomannan in Lesion Tissues for Tuberculosis Diagnosis. Front Cell Infect Microbiol. 2021;11:634915. Published 2021 Mar 15. doi:10.3389/fcimb.2021.634915(IF:5.293)
[3] Liu B, Wang D, Luo E, et al. Role of TG2-Mediated SERCA2 Serotonylation on Hypoxic Pulmonary Vein Remodeling. Front Pharmacol. 2020;10:1611. Published 2020 Feb 11. doi:10.3389/fphar.2019.01611(IF:4.225)
[4] Gong M, Li Z, Zhang X, et al. PTEN mediates serum deprivation-induced cytotoxicity in H9c2 cells via the PI3K/AKT signaling pathway. Toxicol In Vitro. 2021;73:105131. doi:10.1016/j.tiv.2021.105131(IF:3.500)
[5] Zhou X, Chen Q, Huang H, et al. Inhibition of p38 MAPK regulates epileptic severity by decreasing expression levels of A1R and ENT1. Mol Med Rep. 2020;22(6):5348-5357. doi:10.3892/mmr.2020.11614(IF:2.100)
[6] Li GJ, Yang Y, Yang GK, et al. Slit2 suppresses endothelial cell proliferation and migration by inhibiting the VEGF-Notch signaling pathway. Mol Med Rep. 2017;15(4):1981-1988. doi:10.3892/mmr.2017.6240(IF:1.692)
[7] Lai MC, Zhu QQ, Owusu-Ansah KG, et al. Prognostic value of Rho GDP dissociation inhibitors in patients with hepatocellular carcinoma following liver transplantation. Oncol Lett. 2017;14(2):1395-1402. doi:10.3892/ol.2017.6333(IF:1.390)

链霉亲和素是一种来源于阿维丁链霉菌（streptomyces avidinii）的生物素结合蛋白，其非特异性结合远比亲和素低，且与蛋清亲和素在中性pH值具有净正电荷和包含大约7%的碳水化合物相比，链霉亲和素蛋白具有在中性pH值几乎没有净电荷，不含有碳水化合物等更有利的化学性质，所以它被广泛用来作为抗生物素蛋白的替代品。

本品是辣根过氧化物酶标记的链霉亲和素，以液体形式提供，含50%甘油。

建议工作液浓度：0.01-0.1µg/ml for WB（化学发光法）；1-2µg/ml for ELISA,IHC/ICC,WB(底物显色法)。
 

产品性质 

链霉亲和素浓度（Streptavidin Concentration）

0.5 mg/ml

缓冲液（Buffer)

0.005M 磷酸钠，0.125M 氯化钠, pH 7.6

稳定剂（Stabilizer）

7.5mg/ml BSA（无IgG，蛋白酶）

防腐剂（Preservative）

None（叠氮化钠会抑制辣根过氧化物酶的活性）

运输与保存方法

冰袋运输。-20℃保存，有效期1年。避免反复冻融。

注意事项

1) 为了您的安全和健康，请穿实验服并戴一次性手套操作。
2）本产品仅作科研用途！

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35102JP60

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Alexa Fluor® 488标记链霉亲和素 Alexa Fluor® 488 Streptavidin

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Alexa Fluor® 647标记链霉亲和素 Alexa Fluor® 647 Streptavidin  

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HRP Streptavidin 辣根过氧化物酶标记链霉亲和素 HRP链霉亲和素

暂无内容

[1] Wu H, Zeng L, Ou J, Wang T, Chen Y, Nandakumar KS. Estrogen Acts Through Estrogen Receptor-β to Promote Mannan-Induced Psoriasis-Like Skin Inflammation. Front Immunol. 2022;13:818173. Published 2022 May 19. doi:10.3389/fimmu.2022.818173(IF:7.561)
[2] Zhou Y, Xiong H, Chen R, et al. Aptamer Detection of Mycobaterium tuberculosis Mannose-Capped Lipoarabinomannan in Lesion Tissues for Tuberculosis Diagnosis. Front Cell Infect Microbiol. 2021;11:634915. Published 2021 Mar 15. doi:10.3389/fcimb.2021.634915(IF:5.293)
[3] Liu B, Wang D, Luo E, et al. Role of TG2-Mediated SERCA2 Serotonylation on Hypoxic Pulmonary Vein Remodeling. Front Pharmacol. 2020;10:1611. Published 2020 Feb 11. doi:10.3389/fphar.2019.01611(IF:4.225)
[4] Gong M, Li Z, Zhang X, et al. PTEN mediates serum deprivation-induced cytotoxicity in H9c2 cells via the PI3K/AKT signaling pathway. Toxicol In Vitro. 2021;73:105131. doi:10.1016/j.tiv.2021.105131(IF:3.500)
[5] Zhou X, Chen Q, Huang H, et al. Inhibition of p38 MAPK regulates epileptic severity by decreasing expression levels of A1R and ENT1. Mol Med Rep. 2020;22(6):5348-5357. doi:10.3892/mmr.2020.11614(IF:2.100)
[6] Li GJ, Yang Y, Yang GK, et al. Slit2 suppresses endothelial cell proliferation and migration by inhibiting the VEGF-Notch signaling pathway. Mol Med Rep. 2017;15(4):1981-1988. doi:10.3892/mmr.2017.6240(IF:1.692)
[7] Lai MC, Zhu QQ, Owusu-Ansah KG, et al. Prognostic value of Rho GDP dissociation inhibitors in patients with hepatocellular carcinoma following liver transplantation. Oncol Lett. 2017;14(2):1395-1402. doi:10.3892/ol.2017.6333(IF:1.390)

fabgennix HRP-1060说明书

发表于由

fabgennix HRP-1060说明书

兔抗鸡 IgG HRP

产品编号：HRP-1060

一般信息

 

产品

 

抗鸡 IgY HRP

描述

兔抗鸡 IgY（重链和轻链）-马萝卜过氧化物酶

已验证的应用程序

CM、ELISA、IF、IHC、IP、WB

主持人

兔子

物种交叉反应

鸡

免疫原

鸡IgY全分子

物理性质

 

体积

 

500 微升

专注

.5 µg/µl 抗体稳定缓冲液

形式

液体

免疫球蛋白

IgY 重链和轻链

克隆性

多克隆

贮存

储存在 -20⁰C。从 -20⁰C 中取出后，无需解冻即可立即将偶联物用于移液。 

存储缓冲区

抗血清在含有 0.02%硫柳汞或硫柳汞作为防腐剂的抗体稳定缓冲液中提供。

应用协议

 

共聚焦显微镜

 

1:250-1:5,000

D B

1:30,000-1:75,000

酶联免疫吸附试验

1:30,000-1:75,000

免疫细胞化学

1:250-1:5,000

免疫荧光

1:250-1:5,000

免疫组化

1:250-1:5,000

免疫沉淀

1:250-1:5,000

蛋白质印迹

1:4,500-1:2 0,000

 

小鼠抗人IgG4 Fc-HRP单克隆抗体 说明书

发表于由

货号：9200-05

产品描述：Mouse Anti-Human IgG4 Fc-HRP （小鼠抗人IgG4 Fc-HRP单克隆抗体）

规格：1.0ml

品牌：SouthernBiotech 

Southernbiotech专门致力于开发、生产、净化、共轭、和商品化世界上zui高质量的抗体供研究使用。

 

克隆号

HP6025

同型

小鼠（BALB / c）中的IgG 1 K

同种型对照

小鼠IgG 1 -HRP

免疫原

人IgG 4骨髓瘤蛋白质

特异性

人IgG 4 Fc

格式/共轭

HRP（辣根过氧化物酶）

缓冲液配方

50%甘油/ 50%PBS（pH 7.4）; 没有添加防腐剂

规格

1.0毫升

存储和处理

2-8°C储存

应用

ELISA – 质量检测

FLISA – 质量检测
流式细胞仪

免疫组化 – 冷冻切片

免疫组化 – 石蜡切片

免疫细胞化学

蛋白质印迹 

微阵列

Multiplex

纯化 

ELISA板用纯化的人IgG1，IgG2，IgG3和IgG4包被。用连续稀释的小鼠抗人IgG4 Fc-HRP（SB目录号9200-05）检测免疫球蛋白。

产品链接：https://www.southernbiotech.com/?catno=9200-05&type=Monoclonal

 

calpro ALP / HRP说明书

发表于由

 

钙卫蛋白

钙抑素的生物学

钙卫蛋白是在体内普遍发现的36kDa蛋白质。“蛋白质”属于S 100蛋白质家族，具有两个14 kDa（MRP14，S100 A9）和一个8 kDa（MRP8，S100 A8）氨基酸链。该蛋白质在髓样分化过程中表达，在嗜中性粒细胞和单核细胞中含量丰富。在骨髓中发育的髓样细胞大量迁移到血液中，并在许多疾病和病症中进一步迁移到组织中，例如囊性纤维化，类风湿性关节炎以及许多炎症和恶性疾病。

钙卫蛋白是具有许多“特征”的非常丰富的嗜中性粒细胞蛋白，
它占嗜中性粒细胞的细胞溶质中蛋白质总量的60％以上，每个嗜中性粒细胞都含有25皮克钙卫蛋白，而每个红细胞中血红蛋白约为30皮克。

钙卫蛋白从活化的白细胞释放，导致血浆，血清，脊髓液，滑液，尿液，唾液或粪便中的浓度在细菌感染或相关器官发炎期间增加。

钙卫蛋白是抗微生物的，并且在所有测试的细胞类型（人类，动物，正常，恶性）中均诱导了细胞凋亡。这些影响可以通过添加锌来逆转。通过结合钙卫蛋白锌可以抑制许多重要的金属蛋白酶。
缺乏钙卫蛋白与生命不相容。

钙抑素的临床方面

钙卫蛋白综合征的临床表现提示锌缺乏症：儿童/年轻人表现出发育迟缓，骨髓抑制，免疫缺陷，肝脾肿大，关节炎和血管炎；他们血浆中钙卫蛋白的浓度是正常人的2000至12000倍。这些大量的钙卫蛋白结合了太多的锌，导致出现功能性锌缺乏症。

钙卫蛋白是一种疾病标志物：

  1. 为了获得钙卫蛋白的代表性血值，建议在EDTA上采集血样。血清中的浓度通常较高，变化更大。凝结过程中释放了大量的钙卫蛋白。
    体液：随着感染和炎症的发生，血药浓度会升高。血浆钙卫蛋白水平反映出类风湿关节炎和LED的疾病活动性优于ESR和CRP。
  2. 粪便样品：用于测定粪便钙卫蛋白浓度的CalproLab，钙卫蛋白ELISA试验或CalproSmart已成为检测胃肠道炎症或瘤形成，评估炎症性肠病活性和对治疗反应的常规客观和非侵入性测定方法。它也可以预测患者在临床缓解中复发的风险；正常的定量钙卫蛋白值是已经完成粘膜愈合的证据。钙卫蛋白评估的使用有助于选择患者进行内窥镜检查，尤其是在需要全身麻醉的儿童中。

calpro ALP / HRP说明书

Calprolab（ALP / HRP）

CalproLab™ELISA测试（ALP / HRP）

分析：定量ELISA

CalproLab™ELISA试剂盒是用于测定粪便，血液和组织液中钙卫蛋白浓度的定量测定法

CalproLab™ELISA，CE标记，产品编号CALP0170

  • 定量ELISA测试
  • 宽范围ELISA测试（25-2500 mg / kg，仅稀释一种）
  • 120-130分钟直到答案
  • 大量样品分析的理想选择
  • Dynex DS2 ELISA和其他自动化系统可用的协议
  • 小巧且用户友好的套件尺寸
  • 区分IBD和IBS的可靠工具
  • 评估IBD中的炎症活动
  • 监测对IBD治疗的反应
  • IBD黏膜愈合的评估
  • IBD复发的预测因子

 

该测定方法已得到欧洲*的诊所和实验室的验证。

CalproLab™ELISA试剂盒也已通过验证，可以测试血浆/血清样品，从而能够评估风湿性疾病以及区分细菌和病毒感染。