过氧化物酶HRP标记山羊抗兔IgG抗体|Peroxidase-Conjugated Goat Anti-Rabbit IgG

过氧化物酶HRP标记山羊抗兔IgG抗体|Peroxidase-Conjugated Goat Anti-Rabbit IgG

产品说明书

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已发表文献

产品简介

本品是由辣根过氧化酶(HRP)标记的山羊抗兔IgG(H+L),使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子,也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应,但不会识别非免疫球蛋白类的血清蛋白。

本品适合做单标实验。要做多标(Multiple-labeling)实验,建议使用经过与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

 

产品应用

建议稀释浓度:

IHC/ICC:1:500-1:5000;

ELISA/WB(显色法):1:5000-1:100000;

ELISA/WB(化学发光法):1:10000-1:200000;

 

产品信息

抗体浓度(Antibody Concentration)

100 μL(0.4 mg/mL)

缓冲液(Buffer)

0.005M 磷酸钠,0.125M 氯化钠,pH7.6

稳定剂(Stabilizer)

7.5 mg/mL BSA(无IgG,蛋白酶), 50%甘油

防腐剂(Preservative)

None(叠氮化钠会抑制HRP活性)

原料来源(Source of Material)

Jackson Immunoresearch 111-035-003

 

储存条件

-25 ~ -15℃分装保存,尽量避免反复冻融。有效期1年。

 

注意事项

1)本品不能保存于叠氮化钠中其会抑制辣根过氧化酶(HRP)活性;

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

3)本产品仅作科研用途!

 

过氧化物酶HRP标记山羊抗兔IgG抗体|Peroxidase-Conjugated Goat Anti-Rabbit IgG

暂无内容

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[2] Xia B, Shen X, He Y, et al. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4(IF:25.617)
[3] Yang WQ, Xiong QP, Ge JY, et al. THUMPD3-TRMT112 is a m2G methyltransferase working on a broad range of tRNA substrates. Nucleic Acids Res. 2021;49(20):11900-11919. doi:10.1093/nar/gkab927(IF:16.971)
[4] Qian Y, Liu Q, Li P, et al. Highly Tumor-Specific and Long-Acting Iodine-131 Microbeads for Enhanced Treatment of Hepatocellular Carcinoma with Low-Dose Radio-Chemoembolization. ACS Nano. 2021;15(2):2933-2946. doi:10.1021/acsnano.0c09122(IF:15.881)
[5] Wang K, Jiang L, Hu A, et al. Vertebral-specific activation of the CX3CL1/ICAM-1 signaling network mediates non-small-cell lung cancer spinal metastasis by engaging tumor cell-vertebral bone marrow endothelial cell interactions. Theranostics. 2021;11(10):4770-4789. Published 2021 Mar 4. doi:10.7150/thno.54235(IF:11.556)
[6] Han L, Lai H, Yang Y, et al. A 5'-tRNA halve, tiRNA-Gly promotes cell proliferation and migration via binding to RBM17 and inducing alternative splicing in papillary thyroid cancer. J Exp Clin Cancer Res. 2021;40(1):222. Published 2021 Jul 5. doi:10.1186/s13046-021-02024-3(IF:11.161)
[7] Gao JP, Xu P, Wang M, et al. Nod factor receptor complex phosphorylates GmGEF2 to stimulate ROP signaling during nodulation. Curr Biol. 2021;31(16):3538-3550.e5. doi:10.1016/j.cub.2021.06.011(IF:10.834)
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[23] Wang C, Zhang J, Song S, et al. Imaging epileptic foci in mouse models via a low-density lipoprotein receptor-related protein-1 targeting strategy. EBioMedicine. 2021;63:103156. doi:10.1016/j.ebiom.2020.103156(IF:5.736)
[24] Xu R, Lai C, Yang F, et al. Preliminary Characterization of Two Small Insulinase-Like Proteases in Cryptosporidium parvum. Front Microbiol. 2021;12:651512. Published 2021 May 21. doi:10.3389/fmicb.2021.651512(IF:5.640)
[25] Su J, Jin C, Wu H, et al. Differential Expression of Three Cryptosporidium Species-Specific MEDLE Proteins. Front Microbiol. 2019;10:1177. Published 2019 May 29. doi:10.3389/fmicb.2019.01177(IF:5.640)
[26] Zhang S, Wang Y, Wu H, et al. Characterization of a Species-Specific Insulinase-Like Protease in Cryptosporidium parvum. Front Microbiol. 2019;10:354. Published 2019 Mar 6. doi:10.3389/fmicb.2019.00354(IF:5.640)
[27] Yang R, Li D, Yi S, Wang M. Evolutionarily conserved odorant-binding proteins participate in establishing tritrophic interactions. iScience. 2022;25(7):104664. Published 2022 Jun 23. doi:10.1016/j.isci.2022.104664(IF:5.458)
[28] Fang DD, Tang Q, Kong Y, et al. MDM2 inhibitor APG-115 exerts potent antitumor activity and synergizes with standard-of-care agents in preclinical acute myeloid leukemia models. Cell Death Discov. 2021;7(1):90. Published 2021 May 3. doi:10.1038/s41420-021-00465-5(IF:5.241)
[29] Ma Y, Zheng K, Pang Y, et al. Anti-hypertrophic effect of synovium-derived stromal cells on costal chondrocytes promotes cartilage repairs. J Orthop Translat. 2021;32:59-68. Published 2021 Jun 2. doi:10.1016/j.jot.2021.05.002(IF:5.191)
[30] Wu X, Jin S, Yang Y, et al. Altered expression of ferroptosis markers and iron metabolism reveals a potential role of ferroptosis in vitiligo. Pigment Cell Melanoma Res. 2022;35(3):328-341. doi:10.1111/pcmr.13032(IF:4.693)
[31] Li Z, Liu S, Fu T, Peng Y, Zhang J. Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells. Stem Cell Res Ther. 2019;10(1):351. Published 2019 Nov 27. doi:10.1186/s13287-019-1441-4(IF:4.627)
[32] Shi X, Ding K, Zhao Q, et al. Suppression of CPSF6 Enhances Apoptosis Through Alternative Polyadenylation-Mediated Shortening of the VHL 3'UTR in Gastric Cancer Cells. Front Genet. 2021;12:707644. Published 2021 Sep 14. doi:10.3389/fgene.2021.707644(IF:4.599)
[33] Chen Z, Li Z, Jiang C, Jiang X, Zhang J. MiR-92b-3p promotes neurite growth and functional recovery via the PTEN/AKT pathway in acute spinal cord injury. J Cell Physiol. 2019;234(12):23043-23052. doi:10.1002/jcp.28864(IF:4.522)
[34] Yao H, Xu K, Zhou J, Zhou L, Wei S. A Tumor Microenvironment Destroyer for Efficient Cancer Suppression. ACS Biomater Sci Eng. 2020;6(1):450-462. doi:10.1021/acsbiomaterials.9b01544(IF:4.511)
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[37] Fang DD, Zhu H, Tang Q, et al. FLT3 inhibition by olverembatinib (HQP1351) downregulates MCL-1 and synergizes with BCL-2 inhibitor lisaftoclax (APG-2575) in preclinical models of FLT3-ITD mutant acute myeloid leukemia. Transl Oncol. 2022;15(1):101244. doi:10.1016/j.tranon.2021.101244(IF:4.243)
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[40] Guo Y, Huo J, Wu D, et al. Simvastatin inhibits the adipogenesis of bone marrow‑derived mesenchymal stem cells through the downregulation of chemerin/CMKLR1 signaling. Int J Mol Med. 2020;46(2):751-761. doi:10.3892/ijmm.2020.4606(IF:4.101)
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[43] Zhang H, Guo F, Qi P, et al. OsHDA710-Mediated Histone Deacetylation Regulates Callus Formation of Rice Mature Embryo. Plant Cell Physiol. 2020;61(9):1646-1660. doi:10.1093/pcp/pcaa086(IF:4.062)
[44] Sun T, Zhang L, Feng J, et al. Characterization of cellular senescence in doxorubicin-induced aging mice. Exp Gerontol. 2022;163:111800. doi:10.1016/j.exger.2022.111800(IF:4.032)
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[51] Cheng Q, Fan X, Liu Y, et al. miR-455-5p regulates circadian rhythms by accelerating the degradation of Clock mRNA. IUBMB Life. 2022;74(3):245-258. doi:10.1002/iub.2587(IF:3.885)
[52] He RZ, Zheng JH, Yao HF, et al. ADAMTS12 promotes migration and epithelial-mesenchymal transition and predicts poor prognosis for pancreatic cancer [published online ahead of print, 2022 Apr 25]. Hepatobiliary Pancreat Dis Int. 2022;S1499-3872(22)00117-5. doi:10.1016/j.hbpd.2022.04.005(IF:3.780)
[53] Wang Y, Wang H, Zhang L, et al. Potential mechanisms of tremor tolerance induced in rats by the repeated administration of total alkaloid extracts from the seeds of Peganum harmala Linn. J Ethnopharmacol. 2020;262:113183. doi:10.1016/j.jep.2020.113183(IF:3.690)
[54] Bu S, Li B, Wang Q, et al. Epithelial ovarian cancer stem‑like cells are resistant to the cellular lysis of cytokine‑induced killer cells via HIF1A‑mediated downregulation of ICAM‑1. Int J Oncol. 2019;55(1):179-190. doi:10.3892/ijo.2019.4794(IF:3.571)
[55] Shen Y, Zhou M, Yan J, et al. miR-200b inhibits TNF-α-induced IL-8 secretion and tight junction disruption of intestinal epithelial cells in vitro. Am J Physiol Gastrointest Liver Physiol. 2017;312(2):G123-G132. doi:10.1152/ajpgi.00316.2016(IF:3.468)
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产品简介

本品是由辣根过氧化酶(HRP)标记的山羊抗兔IgG(H+L),使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子,也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应,但不会识别非免疫球蛋白类的血清蛋白。

本品适合做单标实验。要做多标(Multiple-labeling)实验,建议使用经过与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

 

产品应用

建议稀释浓度:

IHC/ICC:1:500-1:5000;

ELISA/WB(显色法):1:5000-1:100000;

ELISA/WB(化学发光法):1:10000-1:200000;

 

产品信息

抗体浓度(Antibody Concentration)

100 μL(0.4 mg/mL)

缓冲液(Buffer)

0.005M 磷酸钠,0.125M 氯化钠,pH7.6

稳定剂(Stabilizer)

7.5 mg/mL BSA(无IgG,蛋白酶), 50%甘油

防腐剂(Preservative)

None(叠氮化钠会抑制HRP活性)

原料来源(Source of Material)

Jackson Immunoresearch 111-035-003

 

储存条件

-25 ~ -15℃分装保存,尽量避免反复冻融。有效期1年。

 

注意事项

1)本品不能保存于叠氮化钠中其会抑制辣根过氧化酶(HRP)活性;

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

3)本产品仅作科研用途!

 

过氧化物酶HRP标记山羊抗兔IgG抗体|Peroxidase-Conjugated Goat Anti-Rabbit IgG

暂无内容

[1] Wang Z, Lu Z, Lin S, et al. Leucine-tRNA-synthase-2-expressing B cells contribute to colorectal cancer immunoevasion. Immunity. 2022;55(6):1067-1081.e8. doi:10.1016/j.immuni.2022.04.017(IF:43.474)
[2] Xia B, Shen X, He Y, et al. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4(IF:25.617)
[3] Yang WQ, Xiong QP, Ge JY, et al. THUMPD3-TRMT112 is a m2G methyltransferase working on a broad range of tRNA substrates. Nucleic Acids Res. 2021;49(20):11900-11919. doi:10.1093/nar/gkab927(IF:16.971)
[4] Qian Y, Liu Q, Li P, et al. Highly Tumor-Specific and Long-Acting Iodine-131 Microbeads for Enhanced Treatment of Hepatocellular Carcinoma with Low-Dose Radio-Chemoembolization. ACS Nano. 2021;15(2):2933-2946. doi:10.1021/acsnano.0c09122(IF:15.881)
[5] Wang K, Jiang L, Hu A, et al. Vertebral-specific activation of the CX3CL1/ICAM-1 signaling network mediates non-small-cell lung cancer spinal metastasis by engaging tumor cell-vertebral bone marrow endothelial cell interactions. Theranostics. 2021;11(10):4770-4789. Published 2021 Mar 4. doi:10.7150/thno.54235(IF:11.556)
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