Jackson ImmunoResearch抗FITC抗体

发表于2024年4月21日由上海金畔生物科技有限公司

Jackson ImmunoResearch抗FITC抗体

简要描述：美国Jackson ImmunoResearch Laboratories, Inc. 专业致力于亲合纯化的第二抗体和纯化的免疫球蛋白的生产和偶联。

详细介绍

产品咨询

美国Jackson ImmunoResearch Laboratories, Inc. 专业致力于亲合纯化的第二抗体和纯化的免疫球蛋白的生产和偶联。其产品深受广大研究人员的认可，销售范围遍布，领域包括植物，动物和生物医学研究。公司成立于1982年，公司创业者具有深厚的专业背景：细胞生物学，蛋白化学，微生物学，免疫学。公司自创立初，就将为其他研究人员提供zui全的二抗作为公司的持续发展目标。

Probe	Code Number	Size	Price
Unconjugated	200-002-037	1.0 mg	$ 102
DyLight 405 （A=400, E=421）	200-472-037	0.5 mg	167
Aminomethylcoumarin, AMCA （A=350, E=450）	200-152-037	0.5 mg	132
Alexa®Fluor 488 （A=493, E=519） NEW	200-542-037	0.5 mg	167
Cy 3, Indocarbocyanine （A=550, E=570）	200-162-037	0.5 mg	145
Tetramethyl Rhodamine, TRITC （A=550, E=570）	200-022-037	0.5 mg	114
Rhodamine Red-X, RRX （A=570, E=590）	200-292-037	0.5 mg	114
Alexa®Fluor 594 （A=591, E=614） NEW	200-582-037	0.5 mg	167
DyLight 594 （A=591, E=616）	200-512-037	0.5 mg	167
Texas Red, TR （A=596, E=620）	200-072-037	0.5 mg	139
Alexa®Fluor 647 （A=651, E=667） NEW	200-602-037	0.5 mg	167
DyLight 649 （A=652, E=670）	200-492-037	0.5 mg	167
Cy5, Indodicarbocyanine （A=650, E=670）	200-172-037	0.5 mg	145
Biotin-SP （long spacer）	200-062-037	0.5 ml	130
Horseradish Peroxidase	200-032-037	0.5 ml	130
Alkaline Phosphatase	200-052-037	0.5 ml	144

Annexin V-FITC/PI细胞凋亡检测试剂盒|Annexin V-FITC/PI Apoptosis Detection Kit

发表于2024年3月22日由上海金畔生物科技有限公司

Annexin V-FITC/PI细胞凋亡检测试剂盒|Annexin V-FITC/PI Apoptosis Detection Kit

产品说明书

FAQ

COA

已发表文献

产品描述

Annexin V-FITC/PI细胞凋亡检测试剂盒是用FITC标记的Annexin V作为探针，来检测细胞早期凋亡的发生。

其检测原理为：在正常的活细胞中，磷脂酰丝氨酸（phosphotidylserine，PS）位于细胞膜的内侧，但在早期凋亡的细胞中，PS 从细胞膜的内侧翻转到细胞膜的表面，暴露在细胞外环境中。Annexin-Ⅴ（膜联蛋白-V）是一种分子量为35-36 kDa的Ca²⁺ 依赖性磷脂结合蛋白，能与PS高亲和力结合，可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜结合。

另外，本试剂盒中还提供了碘化丙啶（Propidium Iodide，PI）用来区分存活的早期细胞和坏死或晚期凋亡细胞。PI是一种核酸染料，它不能透过正常细胞或早期凋亡细胞的完整的细胞膜，但可以透过凋亡晚期和坏死细胞的细胞膜而使细胞核染红。因此，将Annexin V与PI联合使用时，PI 则被排除在活细胞（Annexin V-/PI-）和早期凋亡细胞（Annexin V+/PI-）之外，而晚期凋亡细胞和坏死细胞同时被FITC 和PI 结合染色呈现双阳性（Annexin V+/PI+）。

本试剂盒可用于流式细胞仪、荧光显微镜进行检测。

产品组分

编号	组分	产品编号/规格
编号	组分	40302ES20（20T）	40302ES50（50T）	40302ES60（100T）
40302-A	Annexin V-FITC	100 μL	250 μL	500 μL
40302-B	PI Staining Solution	200 μL	500 μL	1.0 mL
40302-C	1×Binding Buffer	10 mL	25 mL	50 mL

运输与保存方法

冰袋（wet ice）运输。-20℃避光保存，避免反复冻融，一年有效。

【注】：如果需要在短时间内多次重复使用，可以在4℃避光保存，半年有效。

注意事项

1）由于细胞凋亡是一个快速的过程，建议样品在染色后1小时之内进行分析。

2）对于贴壁细胞，消化是一个关键步骤。贴壁细胞诱导细胞凋亡时如有漂浮细胞，需收集漂浮细胞和贴壁细胞后合并染色。处理贴壁细胞时要小心操作，尽量避免人为的损伤。胰酶消化时间过短，细胞需要用力吹打才能脱落，容易造成细胞膜的损伤；PI摄入过多，消化时间过长，细胞膜同样易造成损伤，甚至会影响细胞膜上磷脂酰丝氨酸与Annexin V-FITC的结合。消化时将胰酶铺满孔板底后，轻摇使胰酶与细胞充分接触，然后倒掉大部分胰酶，利用剩余少量胰酶再消化一段时间，待细胞间空隙增大，瓶底呈花斑状即可终止。在消化液中尽量不用EDTA，EDTA会影响Annexin V与PS的结合。

3）如果样品来源于血液，请务必除去血液中的血小板。因为血小板含有PS，能与Annexin V结合，从而干扰实验结果。可以使用含有EDTA的缓冲剂并在200 g离心洗去血小板。

4）试剂在开盖前请短暂离心，将盖内壁上的液体甩至管底，避免开盖时液体洒落。

5）Annexin V-FITC和PI是光敏物质，在操作时请注意避光。

6）本产品仅作科研用途！

操作方法

1.1 样品染色

1）悬浮细胞：300 g，4℃离心5 min收集细胞。

贴壁细胞：用不含EDTA的胰酶消化后，300 g，4℃离心5 min收集细胞。胰酶消化时间不宜过长，以防引起假阳性。

2）用预冷的PBS洗涤细胞2次，每次均需300 g，4℃离心5 min。收集1～5×10⁵细胞。

3）吸弃PBS，加入100 μL 1×Binding Buffer重悬细胞。

4）加入5 μL Annexin V-FITC和10 μLPI Staining Solution，轻轻混匀。

5）避光、室温反应10-15 min。

6）加入400 μL 1×Binding Buffer，混匀后放置于冰上，样品在1小时内用流式细胞仪或荧光显微镜检测。

【注】：为了避免洗涤细胞时损失细胞，在吸液时可以用大的Tip头套上小的Tip头吸液。

1.2 样品分析

A．流式细胞仪分析：

FITC最大激发波长为488 nm，最大发射波长525 nm，FITC的绿色荧光在FL1通道检测；PI-DNA复合物的最大激发波长为535 nm，最大发射波长为615 nm，PI的红色荧光在FL2或FL3通道检测。用CellQuest等软件进行分析，绘制双色散点图（two-color dot plot），FITC为横坐标，PI为纵坐标。典型的实验中，细胞可以分成三个亚群，活细胞仅有很低强度的背景荧光，早期凋亡细胞仅有较强的绿色荧光，晚期凋亡细胞有绿色和红色荧光双重染色。

B．荧光显微镜分析：

1）滴一滴用Annexin V-FITC/PI双染的细胞悬液于载玻片上，并用盖玻片盖上细胞。

【注】：对于贴壁细胞，可直接用盖玻片培养细胞并诱导细胞凋亡。

2）在荧光显微镜下用双色滤光片观察。Annexin V-FITC荧光信号呈绿色，PI荧光信号呈红色。

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Cell Cycle and Apoptosis Analysis Kit 细胞周期与细胞凋亡检测试剂盒	40301ES50	50 T
Cell Cycle and Apoptosis Analysis Kit 细胞周期与细胞凋亡检测试剂盒	40301ES60	100 T
Annexin V-EGFP/PI 细胞凋亡检测试剂盒 Annexin V-EGFP/PI Apoptosis Detection Kit	40303ES20	20 T
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Annexin V-Alexa Fluor 647/PI 细胞凋亡检测试剂盒 Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit	40304ES20	20 T
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Annexin V-Alexa Fluor 488/PI 细胞凋亡检测试剂盒 Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit	40305ES20	20 T
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Annexin V-PE/7-AAD细胞凋亡检测试剂盒 Annexin V-PE/7-AAD Apoptosis Detection Kit	40310ES20	20 T
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HB220609

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产品描述

Annexin V-FITC/PI细胞凋亡检测试剂盒是用FITC标记的Annexin V作为探针，来检测细胞早期凋亡的发生。

本试剂盒可用于流式细胞仪、荧光显微镜进行检测。

产品组分

编号	组分	产品编号/规格
编号	组分	40302ES20（20T）	40302ES50（50T）	40302ES60（100T）
40302-A	Annexin V-FITC	100 μL	250 μL	500 μL
40302-B	PI Staining Solution	200 μL	500 μL	1.0 mL
40302-C	1×Binding Buffer	10 mL	25 mL	50 mL

运输与保存方法

冰袋（wet ice）运输。-20℃避光保存，避免反复冻融，一年有效。

【注】：如果需要在短时间内多次重复使用，可以在4℃避光保存，半年有效。

注意事项

1）由于细胞凋亡是一个快速的过程，建议样品在染色后1小时之内进行分析。

4）试剂在开盖前请短暂离心，将盖内壁上的液体甩至管底，避免开盖时液体洒落。

5）Annexin V-FITC和PI是光敏物质，在操作时请注意避光。

6）本产品仅作科研用途！

操作方法

1.1 样品染色

1）悬浮细胞：300 g，4℃离心5 min收集细胞。

贴壁细胞：用不含EDTA的胰酶消化后，300 g，4℃离心5 min收集细胞。胰酶消化时间不宜过长，以防引起假阳性。

2）用预冷的PBS洗涤细胞2次，每次均需300 g，4℃离心5 min。收集1～5×10⁵细胞。

3）吸弃PBS，加入100 μL 1×Binding Buffer重悬细胞。

4）加入5 μL Annexin V-FITC和10 μLPI Staining Solution，轻轻混匀。

5）避光、室温反应10-15 min。

6）加入400 μL 1×Binding Buffer，混匀后放置于冰上，样品在1小时内用流式细胞仪或荧光显微镜检测。

【注】：为了避免洗涤细胞时损失细胞，在吸液时可以用大的Tip头套上小的Tip头吸液。

1.2 样品分析

A．流式细胞仪分析：

B．荧光显微镜分析：

1）滴一滴用Annexin V-FITC/PI双染的细胞悬液于载玻片上，并用盖玻片盖上细胞。

【注】：对于贴壁细胞，可直接用盖玻片培养细胞并诱导细胞凋亡。

2）在荧光显微镜下用双色滤光片观察。Annexin V-FITC荧光信号呈绿色，PI荧光信号呈红色。

相关产品

产品名称	货号	规格
Cell Cycle and Apoptosis Analysis Kit 细胞周期与细胞凋亡检测试剂盒	40301ES50	50 T
Cell Cycle and Apoptosis Analysis Kit 细胞周期与细胞凋亡检测试剂盒	40301ES60	100 T
Annexin V-EGFP/PI 细胞凋亡检测试剂盒 Annexin V-EGFP/PI Apoptosis Detection Kit	40303ES20	20 T
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Annexin V-Alexa Fluor 647/PI 细胞凋亡检测试剂盒 Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit	40304ES20	20 T
	40304ES50	50 T
	40304ES60	100 T
Annexin V-Alexa Fluor 488/PI 细胞凋亡检测试剂盒 Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit	40305ES20	20 T
	40305ES50	50 T
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Annexin V-PE/7-AAD细胞凋亡检测试剂盒 Annexin V-PE/7-AAD Apoptosis Detection Kit	40310ES20	20 T
	40310ES50	50 T
	40310ES60	100 T

HB220609

Q：Annexin V 和 JC-1、Tunel 细胞凋亡检测的区别？

A: Annexin V 是检测细胞早期凋亡的试剂，JC-1 是检测细胞中期凋亡的试剂、Tunel 是检测细胞晚期凋亡的试剂。

Q：Annexin V 和JC-1、Tunel 细胞凋亡检测的可以应用到植物或是细菌（原核生物）吗？

A：可以，但是需要制备原生质体，因为植物细胞或是细菌（原核生物）含有细胞壁，具体的染液使用剂量只需浸没细胞即可，染色时间对于不同细胞有一定的不同。

Q：40302ES Annexin V-FITC/PI 细胞凋亡检测试剂盒里的PI的浓度是多少呢？

A：20ug/ml。

Q:实验结果如何判断？

A：活细胞（Annexin V-/PI-）

早期凋亡细胞（Annexin V+/PI-）

晚期凋亡细胞和坏死细胞呈现双阳性（Annexin V+/PI+）

裸核（Annexin V-/PI+）

Q: Annexin V和TUNEL有什么区别？

A：末端脱氧核苷酸转移酶 dUTP 缺口末端标记 (TUNEL) 是一种染色方法，用于识别细胞内 DNA 片段化位点——晚期细胞凋亡的标志性特征。它使用酶末端脱氧核苷酸转移酶 (TdT) 将修饰的 dNTP（例如 dUTP）连接到片段化 DNA 链的 3'-羟基末端。 dNTPs 通常用荧光团修饰以促进量化和/或可视化。

Annexin V 染色通过结合由于细胞膜不对称性丧失而暴露在细胞外的 PS 残基来识别细胞凋亡的早期阶段。 Annexin V 通常用 FITC 等荧光团标记，以促进凋亡细胞的检测。

Annexin V-FITC/PI细胞凋亡检测试剂盒|Annexin V-FITC/PI Apoptosis Detection Kit

TUNEL细胞凋亡检测试剂盒(FITC)|TUNEL Apoptosis Detection Kit(FITC)

发表于2024年3月22日由上海金畔生物科技有限公司

TUNEL细胞凋亡检测试剂盒(FITC)|TUNEL Apoptosis Detection Kit(FITC)

产品说明书

FAQ

COA

已发表文献

产品描述

细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200 bp的DNA ladder。

TUNEL (TdT mediated dUTP Nick End Labeling)细胞凋亡检测试剂盒（FITC）可以用来检测组织细胞在凋亡晚期过程中细胞核DNA的断裂情况。其原理是在末端脱氧核糖核苷酸转移酶（Terminal Deoxynucleotidyl Transferase, TdT）的作用下，在基因组DNA断裂时暴露出的3´-羟基（3´-OH）末端掺入FITC-12-dUTP，从而可以用荧光显微镜或流式细胞仪检测。

本试剂盒对标记反应进行了优化，采用最佳比例的FITC-12-dUTP和未标记dNTP进行3’-OH末端的核苷酸掺入，使得同一个断裂的DNA片段末端可以形成更长的“标记尾巴”。该“标记尾巴”减少了相邻掺入dNTP上标记基团的空间位阻，增加每个断裂片段上的荧光基团数目，降低荧光基团相邻后可能造成的聚集和淬灭，从而提高检测灵敏度，减少非特异性反应。

本试剂盒应用范围广，可以用于检测冷冻或石蜡切片中的细胞凋亡情况，也可以检测培养的贴壁细胞或悬浮细胞的凋亡情况。

产品组分

编号	组分	产品编号/规格
编号	组分	40306ES20（20T）	40306ES50（50T）	40306ES60（100T）
40306-A	5×Equilibration Buffer	750 μL	1.25 mL×2	1.25 mL×3
40306-B	FITC-12-dUTP Labeling Mix	100 μL	250 μL	250 μL×2
40306-C	Recombinant TdT Enzyme	20 μL	50 μL	50 μL×2
40306-D	Proteinase K (2 mg/mL)	40 μL	100 μL	100 μL×2
40306-E	DNase I (1 U/ μL)	5 μL	12.5 μL	25 μL
40306-F	10 × DNase I Buffer	100 μL	250 μL	500 μL

运输与保存方法

冰袋（wet ice）运输。

本试剂盒储存在-20℃，FITC-12-dUTP Labling Mix避光储存于-20℃，保质期为二年。

注意事项

1）需自备用于洗涤细胞的PBS，用于封片的抗荧光淬灭封片液，用于固定的4%多聚甲醛。

2）如需染核，需自备DAPI（2 μg/mL）或PI（1 μg/mL）。

3）如果用流式细胞仪，自备PI（1 μg/mL）和DNase Free RNase A。

4）为了您的安全和健康，请穿实验服并戴一次性手套操作。

5）本产品仅作科研用途！

操作步骤

一、样品准备

A. 石蜡包埋组织切片

1. 室温下将石蜡组织切片放入二甲苯中浸泡5 min，重复一次，以彻底脱掉石蜡。

2. 室温下用100%乙醇浸泡切片5 min，重复一次。

3. 室温下用梯度乙醇（90、80、70%）各浸洗1次，每次3 min。

4. 用PBS轻轻润洗切片，并用滤纸小心吸干玻片上样本周围多余的液体。这时，可用石蜡笔或疏水笔在样品周围描绘样品分布的轮廓，便于下游透性处理和平衡标记操作。在实验过程中，切勿让样品干燥，处理好的样本放在湿盒中保持样本的湿润。

5. 配制Proteinase K工作液：按1:100的比例，用PBS作为稀释液来稀释2 mg/mL的Proteinase K溶液，使其终浓度为20 μg/mL。

6. 每个样本上滴加100 μL上述Proteinase K工作液，使其被全部覆盖，室温孵育20 min。

注：Proteinase K帮助组织和细胞对后续步骤的染色试剂通透。孵育时间过长会增加组织切片在后续洗涤步骤中从载波片上脱落的风险，过短则可能造成透性处理不充分，影响标记效率。未得到更好的结果，可能需要优化Proteinase K孵育的时间。

7. 用PBS溶液润洗样本，轻轻去掉多余液体，并用滤纸小心吸干载玻片上样本周围的液体。处理后的样本放在湿盒中保存样本的湿润。

B. 组织冰冻切片

1. 将玻片浸没在4%多聚甲醛溶液（溶于PBS）中固定，室温下孵育15 min。

2. 轻轻去掉多余液体，并用滤纸小心吸干玻片上样本周围多余的液体。

3. 将玻片浸没在PBS溶液中，室温孵育15 min。

4. 轻轻去掉多余液体，并用滤纸小心吸干玻片上样本周围多余的液体。这时，可用石蜡笔或疏水笔在样品周围描绘样品分布的轮廓，便于下游透性处理和平衡标记操作。在实验过程中，切勿让样品干燥，处理好的样本放在湿盒中保持样本的湿润。

5. 配制Proteinase K工作液：按1:100的比例，用PBS作为稀释液来稀释2 mg/mL的Proteinase K溶液，使其终浓度为20 μg/mL。

6. 每个样本上滴加100 μL上述Proteinase K工作液，使其被全部覆盖，室温孵育10 min。

【注】Proteinase K帮助组织和细胞对后续步骤的染色试剂通透。孵育时间过长会增加组织切片在后续洗涤步骤中从载波片上脱落的风险，过短则可能造成透性处理不充分，影响标记效率。未得到更好的结果，可能需要优化Proteinase K孵育的时间。

7. 用PBS溶液润洗样本2-3次。

8. 轻轻去掉多余液体，并用滤纸小心吸干载玻片上样本周围的液体。处理后的样本放在湿盒中保存样本的湿润。

C. 细胞样品

【细胞爬片的准备】

在Lab-Tek载玻片小室（Chamber Slides）上培养贴壁细胞。在凋亡诱导处理之后，用PBS洗2遍载玻片。

【细胞涂片的制备（以多聚赖氨酸包被的载玻片为例）】

1. 准备多聚赖氨酸包被的载玻片：吸取50–100 μL 0.01% (w/v)多聚赖氨酸水溶液，滴至每一片预清洗过的玻璃载玻片的表面。在将要用于固定细胞的区域将多聚赖氨酸溶液涂散为一薄层。待载玻片晾干之后，迅速用去离子水漂洗，然后让包被后的载玻片在空气中晾干30-60 min。包被后的载玻片能在室温储存数月。

2. 以约2×10⁷个细胞/mL的浓度将细胞重悬于PBS中，吸取50-100 μL细胞悬液滴于多聚赖氨酸包被的载玻片上，用一片干净的载玻片轻柔的涂开细胞悬液。

按照以下步骤对细胞样品进行处理：

1. 固定细胞，将载玻片浸入装有4%新鲜配制于PBS中的多聚甲醛的染色缸中，在4℃放置25 min。

2. 洗涤载玻片，将其浸入PBS中，室温放置5 min。重复用PBS洗一次。

3. 轻轻去掉多余液体，并用滤纸小心吸干玻片上样本周围多余的液体。这时，可用石蜡笔或指甲油在样品周围描绘样品分布的轮廓，便于下游透性处理和平衡标记操作。在实验过程中，切勿让样品干燥，处理好的样本放在湿盒中保持样本的湿润。

4. 每个样本上可浸于0.2%配制于PBS中的Triton X-100溶液中，室温孵育5 min进行通透处理（Proteinase K处理容易使细胞脱落）。

5. 在盛有PBS溶液的敞口烧杯中浸没清洗样本2-3次。

6. 轻轻去掉多余液体，并用滤纸小心吸干载玻片上样本周围的液体。处理后的样本放在湿盒中保存样本的湿润。

二、DNA酶处理阳性对照的步骤（可选）

在样本通透处理后，用DNA酶I处理细胞来准备阳性对照载玻片。该流程通常会引起被处理的大多数细胞显现绿色荧光。

【注】：DNA酶I处理固定的细胞会引起染色体DNA的断裂，产生许多可标记的DNA 3’-末端。

1. 按1:10的比例用去离子水稀释10×DNase I Buffer(每个样本需用200 μL 1×DNase I Buffer，即需要用20 μL 10×DNase I Buffer和180 μL去离子水混合稀释)，取其中100 μL滴加到已通透的样本上，室温孵育5 min。向剩余100 μL 1×DNase I Buffer中加1 μL DNase I (1U/μL)，使其终浓度为10 U/mL。轻叩掉液体，加入100 μL含5.5-10 units/mL DNase I的缓冲液，室温孵育10 min。

2. 轻轻叩掉液体，加入100 μL 含10 U/mL DNase I 的缓冲液，室温孵育10 min。

3. 轻叩载玻片，去掉多余的液体，并将载玻片在装有去离子水的染色缸中彻底洗3-4次。

【注】：阳性对照载玻片必须使用单独的染色缸，否则阳性对照载玻片上残余的DNase I 可能会在实验载玻片上引入高背景。

三、标记与检测

1. 按1:5的比例用去离子水稀释5×Equilibration Buffer。

2. 每个样本滴加100 μL 1×Equilibration Buffer使其全部覆盖待检样本区域，室温孵育10-30 min。或者将载玻片放入一个含有 1×Equilibration Buffer的缸中，保证缓冲液没过样本。在平衡细胞的同时在冰上解冻FITC-12-dUTP Labling Mix，并且依照表1，准备足够量的用于所有实验的和可选阳性对照反应的TdT孵育缓冲液。对于面积小于5 cm²的一个标准反应，其体积是50 μL，用50 μL乘以实验和阳性对照反应的数目来确定所需TdT孵育缓冲液的总体积。对于表面积更大的样本，可成比例的增大试剂体积。

表1. 准备用于实验的和可选阳性对照反应的TdT孵育缓冲液

组分	体积（μL /50 μL体系）
ddH₂O	34
5×Equilibration Buffer	10
FITC-12-dUTP Labling Mix	5
Recombinant TdT Enzyme	1

阴性对照体系：准备一份不含TdT酶的对照孵育缓冲液，用ddH₂O替代TdT酶。

3. 在平衡后的区域周围用吸水纸洗掉100 μL 1×Equilibration Buffer中的大部分，然后在5 cm²面积的细胞上加入50 μL TdT孵育缓冲液。不要让细胞干掉。这之后的操作，载玻片要避光。

4. 把塑料盖玻片盖在细胞上以保证试剂的平均分布，在湿盒的底部放上用水浸湿的纸巾。将载玻片置于湿盒内，在37℃孵育60 min。将湿盒用铝箔纸包裹以避光。

注：塑料盖玻片在使用前可以切成两半。折起盖玻片的边缘以便于移除和操作。

5. 移除塑料盖玻片，并将切片置于PBS溶液中室温孵育5 min。

6. 轻轻去掉多余液体，换用新鲜的PBS溶液室温孵育5 min，重复一次。

7. 用滤纸轻轻擦掉样本周围及背面的PBS溶液。注意：为了降低背景，载玻片在用PBS洗一遍后，可再用含0.1% Triton X-100和5 mg/mL BSA的PBS洗3次，每次5 min，这样可将游离的未反应标记物清除干净。

8. 样本在染色缸中染色，在黑暗中将载玻片浸入装有PI溶液（1 μg/mL，用PBS新鲜配制并稀释）的染色缸，室温放置5 min。可选操作：样本在染色缸中染色，在黑暗中将载玻片浸入装有DAPI溶液（2 μg/mL，用PBS新鲜配制并稀释）的染色缸，室温放置5 min。

9. 洗涤样本，将载玻片浸入去离子水中，室温放置5 min，重复2次，总共洗3次。

10. 叩干载玻片上多余的水并且用吸水纸擦拭细胞周边的区域。

11. 立即在荧光显微镜下分析样本，用标准的荧光过滤装置在520±20 nm的荧光下观察绿色荧光；在＞620 nm下观察PI的红色荧光，或在460 nm观察蓝色的DAPI。如有必要，载玻片能在4℃黑暗条件下存放过夜。PI/DAPI能将凋亡和未凋亡的细胞都染成红色/蓝色，只在凋亡的细胞核中才有FITC-12-dUTP掺入而定位的绿色荧光。

四、利用流式细胞术检测悬浮细胞

1. 将3-5×10⁶个细胞用PBS在4℃离心（300×g）洗两次，然后重悬在0.5 mL PBS中。

2. 固定细胞，加入5 mL 1%配制于PBS中的多聚甲醛溶液，冰上放置20 min。

3. 细胞在4℃，300×g离心10 min，去上清并且重悬于5mL PBS。重复洗一次，并用0.5 mL PBS重悬细胞。

4. 通透细胞，加入5 mL冰上预冷的70%乙醇，在-20℃孵育4小时。细胞能在70%乙醇中-20℃条件下保存一周，或者，细胞可用配制于PBS中的0.2% Triton X-100溶液通透，室温放置5 min。

5. 细胞在300×g离心10 min，并用5 mL PBS重悬。重复离心，并1 mL PBS重悬。

6. 转移2×10⁶个细胞至一个1.5 mL的微量离心管。

7. 300×g离心10 min，去上清，并用80 μL 1×Equilibration Buffer重悬。室温孵育5 min。

8. 在平衡细胞的同时，在冰上融解FITC-12-dUTP标记混合物，并且依照表1，准备足够量的用于所有反应的TdT孵育缓冲液。对于2×10⁶个细胞的一个标准反应，其体积是50 μL，用50μl乘上反应数目来确定所需TdT孵育缓冲液的总体积。

9. 细胞在300×g离心10 min，去上清并把沉淀重悬在50 μL TdT孵育缓冲液中，37℃孵育60 min，避光。每隔15 min用微量移液器轻轻重悬细胞。

10. 加入1mL ,20 mM EDTA终止反应，用微量移液器轻柔混匀。

11. 300×g离心10 min，去上清并把沉淀重悬在1mL配制于PBS中的0.1% Triton X-100溶液，其中含5 mg/mL BSA，重复一次，总共洗2次。

12. 300×g离心10 min，去上清并把细胞沉淀重悬在0.5 mL PI溶液（1 μg/mL）中，其中包含250 μg 无DNA酶的Rnase A。

13. 在黑暗中室温孵育细胞30 min。

14. 用流式细胞仪分析细胞，测量520±20 nm的FITC-12-dUTP的绿色荧光和＞620 nm的PI红色荧光。PI将凋亡和未凋亡的细胞都染成红色，只在凋亡细胞核中才有FITC-12-dUTP掺入而定位的绿色荧光。

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产品名称	产品编号	规格
Annexin V-FITC/PI 细胞凋亡检测试剂盒	40302ES20	20 T
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Annexin V-EGFP/PI 细胞凋亡检测试剂盒	40303ES20	20 T
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Annexin V-Alexa Fluor 647/PI 细胞凋亡检测试剂盒	40304ES20	20 T
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Annexin V-Alexa Fluor 488/PI 细胞凋亡检测试剂盒	40305ES20	20 T
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TUNEL细胞凋亡检测试剂盒（FITC）	40306ES20	20 T
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TUNEL细胞凋亡检测试剂盒（Alexa Fluor 488）	40307ES20	20 T
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TUNEL细胞凋亡检测试剂盒（Alexa Fluor 640）	40308ES20	20 T
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HB210715

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产品描述

细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200 bp的DNA ladder。

本试剂盒应用范围广，可以用于检测冷冻或石蜡切片中的细胞凋亡情况，也可以检测培养的贴壁细胞或悬浮细胞的凋亡情况。

产品组分

编号	组分	产品编号/规格
编号	组分	40306ES20（20T）	40306ES50（50T）	40306ES60（100T）
40306-A	5×Equilibration Buffer	750 μL	1.25 mL×2	1.25 mL×3
40306-B	FITC-12-dUTP Labeling Mix	100 μL	250 μL	250 μL×2
40306-C	Recombinant TdT Enzyme	20 μL	50 μL	50 μL×2
40306-D	Proteinase K (2 mg/mL)	40 μL	100 μL	100 μL×2
40306-E	DNase I (1 U/ μL)	5 μL	12.5 μL	25 μL
40306-F	10 × DNase I Buffer	100 μL	250 μL	500 μL

运输与保存方法

冰袋（wet ice）运输。

本试剂盒储存在-20℃，FITC-12-dUTP Labling Mix避光储存于-20℃，保质期为二年。

注意事项

1）需自备用于洗涤细胞的PBS，用于封片的抗荧光淬灭封片液，用于固定的4%多聚甲醛。

2）如需染核，需自备DAPI（2 μg/mL）或PI（1 μg/mL）。

3）如果用流式细胞仪，自备PI（1 μg/mL）和DNase Free RNase A。

4）为了您的安全和健康，请穿实验服并戴一次性手套操作。

5）本产品仅作科研用途！

操作步骤

一、样品准备

A. 石蜡包埋组织切片

1. 室温下将石蜡组织切片放入二甲苯中浸泡5 min，重复一次，以彻底脱掉石蜡。

2. 室温下用100%乙醇浸泡切片5 min，重复一次。

3. 室温下用梯度乙醇（90、80、70%）各浸洗1次，每次3 min。

5. 配制Proteinase K工作液：按1:100的比例，用PBS作为稀释液来稀释2 mg/mL的Proteinase K溶液，使其终浓度为20 μg/mL。

6. 每个样本上滴加100 μL上述Proteinase K工作液，使其被全部覆盖，室温孵育20 min。

7. 用PBS溶液润洗样本，轻轻去掉多余液体，并用滤纸小心吸干载玻片上样本周围的液体。处理后的样本放在湿盒中保存样本的湿润。

B. 组织冰冻切片

1. 将玻片浸没在4%多聚甲醛溶液（溶于PBS）中固定，室温下孵育15 min。

2. 轻轻去掉多余液体，并用滤纸小心吸干玻片上样本周围多余的液体。

3. 将玻片浸没在PBS溶液中，室温孵育15 min。

5. 配制Proteinase K工作液：按1:100的比例，用PBS作为稀释液来稀释2 mg/mL的Proteinase K溶液，使其终浓度为20 μg/mL。

6. 每个样本上滴加100 μL上述Proteinase K工作液，使其被全部覆盖，室温孵育10 min。

7. 用PBS溶液润洗样本2-3次。

8. 轻轻去掉多余液体，并用滤纸小心吸干载玻片上样本周围的液体。处理后的样本放在湿盒中保存样本的湿润。

C. 细胞样品

【细胞爬片的准备】

在Lab-Tek载玻片小室（Chamber Slides）上培养贴壁细胞。在凋亡诱导处理之后，用PBS洗2遍载玻片。

【细胞涂片的制备（以多聚赖氨酸包被的载玻片为例）】

2. 以约2×10⁷个细胞/mL的浓度将细胞重悬于PBS中，吸取50-100 μL细胞悬液滴于多聚赖氨酸包被的载玻片上，用一片干净的载玻片轻柔的涂开细胞悬液。

按照以下步骤对细胞样品进行处理：

1. 固定细胞，将载玻片浸入装有4%新鲜配制于PBS中的多聚甲醛的染色缸中，在4℃放置25 min。

2. 洗涤载玻片，将其浸入PBS中，室温放置5 min。重复用PBS洗一次。

4. 每个样本上可浸于0.2%配制于PBS中的Triton X-100溶液中，室温孵育5 min进行通透处理（Proteinase K处理容易使细胞脱落）。

5. 在盛有PBS溶液的敞口烧杯中浸没清洗样本2-3次。

6. 轻轻去掉多余液体，并用滤纸小心吸干载玻片上样本周围的液体。处理后的样本放在湿盒中保存样本的湿润。

二、DNA酶处理阳性对照的步骤（可选）

在样本通透处理后，用DNA酶I处理细胞来准备阳性对照载玻片。该流程通常会引起被处理的大多数细胞显现绿色荧光。

【注】：DNA酶I处理固定的细胞会引起染色体DNA的断裂，产生许多可标记的DNA 3’-末端。

2. 轻轻叩掉液体，加入100 μL 含10 U/mL DNase I 的缓冲液，室温孵育10 min。

3. 轻叩载玻片，去掉多余的液体，并将载玻片在装有去离子水的染色缸中彻底洗3-4次。

【注】：阳性对照载玻片必须使用单独的染色缸，否则阳性对照载玻片上残余的DNase I 可能会在实验载玻片上引入高背景。

三、标记与检测

1. 按1:5的比例用去离子水稀释5×Equilibration Buffer。

表1. 准备用于实验的和可选阳性对照反应的TdT孵育缓冲液

组分	体积（μL /50 μL体系）
ddH₂O	34
5×Equilibration Buffer	10
FITC-12-dUTP Labling Mix	5
Recombinant TdT Enzyme	1

阴性对照体系：准备一份不含TdT酶的对照孵育缓冲液，用ddH₂O替代TdT酶。

注：塑料盖玻片在使用前可以切成两半。折起盖玻片的边缘以便于移除和操作。

5. 移除塑料盖玻片，并将切片置于PBS溶液中室温孵育5 min。

6. 轻轻去掉多余液体，换用新鲜的PBS溶液室温孵育5 min，重复一次。

9. 洗涤样本，将载玻片浸入去离子水中，室温放置5 min，重复2次，总共洗3次。

10. 叩干载玻片上多余的水并且用吸水纸擦拭细胞周边的区域。

四、利用流式细胞术检测悬浮细胞

1. 将3-5×10⁶个细胞用PBS在4℃离心（300×g）洗两次，然后重悬在0.5 mL PBS中。

2. 固定细胞，加入5 mL 1%配制于PBS中的多聚甲醛溶液，冰上放置20 min。

3. 细胞在4℃，300×g离心10 min，去上清并且重悬于5mL PBS。重复洗一次，并用0.5 mL PBS重悬细胞。

5. 细胞在300×g离心10 min，并用5 mL PBS重悬。重复离心，并1 mL PBS重悬。

6. 转移2×10⁶个细胞至一个1.5 mL的微量离心管。

7. 300×g离心10 min，去上清，并用80 μL 1×Equilibration Buffer重悬。室温孵育5 min。

9. 细胞在300×g离心10 min，去上清并把沉淀重悬在50 μL TdT孵育缓冲液中，37℃孵育60 min，避光。每隔15 min用微量移液器轻轻重悬细胞。

10. 加入1mL ,20 mM EDTA终止反应，用微量移液器轻柔混匀。

11. 300×g离心10 min，去上清并把沉淀重悬在1mL配制于PBS中的0.1% Triton X-100溶液，其中含5 mg/mL BSA，重复一次，总共洗2次。

12. 300×g离心10 min，去上清并把细胞沉淀重悬在0.5 mL PI溶液（1 μg/mL）中，其中包含250 μg 无DNA酶的Rnase A。

13. 在黑暗中室温孵育细胞30 min。

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	40308ES50	50 T
	40308ES60	100 T

HB210715

Q：共染之后TUNEL 的信号就不在核内了，感觉都弥散了。TUNEL 染色就按照说明书来的，DAPI 之前孵育另外的一抗？

A：可能是后期洗涤次数过多，建议减少洗涤次数，或者洗涤动作轻柔一些。

Q：TUNEL 可以和 DAPI 一起染色细胞吗?

A：可以。

Q：Annexin V 和 JC-1、Tunel 细胞凋亡检测的区别？

A： Annexin V 是检测细胞早期凋亡的试剂，JC-1 是检测细胞中期凋亡的试剂、Tunel 是检测细胞晚期凋亡的试剂。

Q：Annexin V 和JC-1、Tunel 细胞凋亡检测的可以应用到植物或是细菌（原核生物）吗？

Q：Tunel 细胞凋亡检测，细胞爬片好凋亡处理后需要在固定通透吗？

A：需要通透，因为 TdT 酶需要经过通透的细胞才能进入细胞内，而 Annexin V 和 JC-1 是不能进行染色固定的。

Q：Tunel 细胞凋亡检测时，贴壁细胞必须要先消化下来再染色吗？

A：不需要，对于贴壁细胞，要先用PBS 洗 2-3 次，然后直接用多聚甲醛固定细胞，通透处理，染色观察。

Q：固定时间可以增加吗？

A： 4℃放置25 min左右，选择4%多聚甲醛作固定液，乙醇、甲醇、酸性固定液，会导致标记效率低；固定时间不宜过长，过长导致交联程度过高，进而降低标记效率。

FITC标记山羊抗兔IgG抗体 FITC山羊抗兔IgG抗体|FITC Goat Anti-Rabbit IgG

发表于2024年3月10日由上海金畔生物科技有限公司

FITC标记山羊抗兔IgG抗体 FITC山羊抗兔IgG抗体|FITC Goat Anti-Rabbit IgG

产品说明书

FAQ

COA

已发表文献

产品描述

本品是由FITC标记的山羊抗兔IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。该荧光基团光谱性质同Alexa Fluor 488，Amax（最大激发波长）为492nm，Emax（最大发射波长）为520nm。

本品适合做单标实验，广泛应用于多种免疫学实验如免疫细胞化学（ICC），流式细胞术（FC）以及免疫组化（IHC）等。要做多标（multiple-labeling）实验，建议使用与相近物种的血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

产品应用

建议稀释浓度：1:25-1:100（For most application）

产品性质

抗体浓度（Antibody Concentration）	100µl (0.75mg/ml)
缓冲液（Buffer）	0.005M 磷酸钠，0.125M 氯化钠, pH 7.6
稳定剂（Stabilizer）	7.5mg/ml BSA（无IgG，蛋白酶），50% 甘油
荧光素（Fluorophore）	FITC-isomer 1, Amax =492nm, Emax=520nm
防腐剂（Preservative）	0.025% 叠氮化钠
原料来源（Source of Material）	Jackson Immunoresearch 111-095-003

运输与保存方法

冰袋运输。-20℃分装保存，尽量避免反复冻融。有效期1年。

注意事项

1）本品含叠氮化钠，对人体有害，请注意适当防护。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

3）本产品仅作科研用途！

[1] Wang Z, Gong X, Li J, et al. Oxygen-Delivering Polyfluorocarbon Nanovehicles Improve Tumor Oxygenation and Potentiate Photodynamic-Mediated Antitumor Immunity. ACS Nano. 2021;15(3):5405-5419. doi:10.1021/acsnano.1c00033(IF:15.881)
[2] Bao L, Dou G, Tian R, et al. Engineered neutrophil apoptotic bodies ameliorate myocardial infarction by promoting macrophage efferocytosis and inflammation resolution. Bioact Mater. 2021;9:183-197. Published 2021 Aug 27. doi:10.1016/j.bioactmat.2021.08.008(IF:14.593)
[3] Shi X, Cheng Y, Wang J, et al. 3D printed intelligent scaffold prevents recurrence and distal metastasis of breast cancer. Theranostics. 2020;10(23):10652-10664. Published 2020 Aug 29. doi:10.7150/thno.47933(IF:11.556)
[4] Li Z, Wu M, Liu S, et al. Apoptotic vesicles activate autophagy in recipient cells to induce angiogenesis and dental pulp regeneration [published online ahead of print, 2022 May 10]. Mol Ther. 2022;S1525-0016(22)00304-5. doi:10.1016/j.ymthe.2022.05.006(IF:11.454)
[5] Chen H, Wang X, Wang J, et al. In vitroadipogenesis and long-term adipocyte culture in adipose tissue-derived cell banks. Biofabrication. 2021;13(3):10.1088/1758-5090/ac0610. Published 2021 Jul 5. doi:10.1088/1758-5090/ac0610(IF:10.020)
[6] Wang Y, Lin YX, Qiao SL, et al. Polymeric nanoparticles promote macrophage reversal from M2 to M1 phenotypes in the tumor microenvironment. Biomaterials. 2017;112:153-163. doi:10.1016/j.biomaterials.2016.09.034(IF:8.387)
[7] Yuan J, Jiang X, Lan H, et al. Multi-Omics Analysis of the Therapeutic Value of MAL2 Based on Data Mining in Human Cancers. Front Cell Dev Biol. 2022;9:736649. Published 2022 Jan 17. doi:10.3389/fcell.2021.736649(IF:6.684)
[8] Tao Y, Qiao SM, Lv CJ, et al. Phytoestrogen arctigenin preserves the mucus barrier in inflammatory bowel diseases by inhibiting goblet cell apoptosis via the ERβ/TRIM21/PHB1 pathway [published online ahead of print, 2022 May 22]. Phytother Res. 2022;10.1002/ptr.7495. doi:10.1002/ptr.7495(IF:5.882)
[9] Li Z, You L, Yan D, James AA, Huang Y, Tan A. Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination. PLoS Genet. 2018;14(2):e1007245. Published 2018 Feb 23. doi:10.1371/journal.pgen.1007245(IF:5.540)
[10] Tao Y, Yue M, Lv C, et al. Pharmacological activation of ERβ by arctigenin maintains the integrity of intestinal epithelial barrier in inflammatory bowel diseases. FASEB J. 2020;34(2):3069-3090. doi:10.1096/fj.201901638RR(IF:5.391)
[11] Li Z, Liu S, Fu T, Peng Y, Zhang J. Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells. Stem Cell Res Ther. 2019;10(1):351. Published 2019 Nov 27. doi:10.1186/s13287-019-1441-4(IF:4.627)
[12] Li Z, You L, Zhang Q, Yu Y, Tan A. A Targeted In-Fusion Expression System for Recombinant Protein Production in Bombyx mori. Front Genet. 2022;12:816075. Published 2022 Jan 4. doi:10.3389/fgene.2021.816075(IF:4.599)
[13] Zheng C, Wu SM, Lian H, et al. Low-intensity pulsed ultrasound attenuates cardiac inflammation of CVB3-induced viral myocarditis via regulation of caveolin-1 and MAPK pathways. J Cell Mol Med. 2019;23(3):1963-1975. doi:10.1111/jcmm.14098(IF:4.302)
[14] Wang H, Zhang Z, Guan J, Lu W, Zhan C. Unraveling GLUT-mediated transcytosis pathway of glycosylated nanodisks. Asian J Pharm Sci. 2021;16(1):120-128. doi:10.1016/j.ajps.2020.07.001(IF:3.968)
[15] Chen L, Lv L, Zhang L, et al. Metformin ameliorates bladder dysfunction in a rat model of partial bladder outlet obstruction. Am J Physiol Renal Physiol. 2021;320(5):F838-F858. doi:10.1152/ajprenal.00625.2020(IF:3.377)
[16] Yang LY, Liu XF, Yang Y, et al. Biochemical features of the adhesion G protein-coupled receptor CD97 related to its auto-proteolysis and HeLa cell attachment activities. Acta Pharmacol Sin. 2017;38(1):56-68. doi:10.1038/aps.2016.89(IF:3.166)
[17] Wang Y, Jiang C, Cong S, Guo C, Yan Z. Extracellular matrix deposited by Wharton's jelly mesenchymal stem cells enhances cell expansion and tissue specific lineage potential. Am J Transl Res. 2018;10(11):3465-3480. Published 2018 Nov 15. (IF:3.061)
[18] Xue Z, Zhuang J, Bai H, et al. VDR mediated HSD3B1 to regulate lipid metabolism and promoted testosterone synthesis in mouse Leydig cells. Genes Genomics. 2022;44(5):583-592. doi:10.1007/s13258-022-01232-1(IF:1.839)

产品描述

产品应用

建议稀释浓度：1:25-1:100（For most application）

产品性质

抗体浓度（Antibody Concentration）	100µl (0.75mg/ml)
缓冲液（Buffer）	0.005M 磷酸钠，0.125M 氯化钠, pH 7.6
稳定剂（Stabilizer）	7.5mg/ml BSA（无IgG，蛋白酶），50% 甘油
荧光素（Fluorophore）	FITC-isomer 1, Amax =492nm, Emax=520nm
防腐剂（Preservative）	0.025% 叠氮化钠
原料来源（Source of Material）	Jackson Immunoresearch 111-095-003

运输与保存方法

冰袋运输。-20℃分装保存，尽量避免反复冻融。有效期1年。

注意事项

1）本品含叠氮化钠，对人体有害，请注意适当防护。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

3）本产品仅作科研用途！

FITC标记山羊抗兔IgG抗体 FITC山羊抗兔IgG抗体|FITC Goat Anti-Rabbit IgG

暂无内容

FITC标记山羊抗人IgG Goat Anti-Human IgG(H+L)

发表于2024年3月10日由上海金畔生物科技有限公司

FITC标记山羊抗人IgG Goat Anti-Human IgG(H+L)

产品说明书

FAQ

COA

已发表文献

产品信息

产品名称	产品编号	规格
FITC-AffiniPure Goat Anti-Human IgG(H+L)	34856JP60	100 μL

产品简介

本品是由FITC标记的山羊抗人IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的人IgG分子，也会与其他人免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。该荧光基团光谱性质同Alexa Fluor 488，Amax（最大激发波长）为492nm，Emax（最大发射波长）为520nm。

本品适合做单标实验，广泛应用于多种免疫学实验如免疫细胞化学（ICC），流式细胞术（FC）以及免疫组化（IHC）等。要做多标（multiple-labeling）实验，建议使用与相近物种的血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。。

产品信息

抗体浓度（Antibody Concentration）	100 µL (0.75 mg/ml)
缓冲液（Buffer）	0.005M 磷酸钠，0.125M 氯化钠，pH7.6
稳定剂（Stabilizer）	7.5mg/ml BSA（无IgG，无蛋白酶）
防腐剂（Preservative）	0.025%叠氮化钠

产品应用

建议稀释浓度：1:25-1:100(for most application)

储存条件

-25 ~ -15℃分装保存，尽量避免反复冻融。

注意事项

1）为了您的安全和健康，请穿实验服并戴一次性手套操作；

2）本产品仅作科研用途！

Ver.CN20240228

产品信息

产品名称	产品编号	规格
FITC-AffiniPure Goat Anti-Human IgG(H+L)	34856JP60	100 μL

产品简介

产品信息

抗体浓度（Antibody Concentration）	100 µL (0.75 mg/ml)
缓冲液（Buffer）	0.005M 磷酸钠，0.125M 氯化钠，pH7.6
稳定剂（Stabilizer）	7.5mg/ml BSA（无IgG，无蛋白酶）
防腐剂（Preservative）	0.025%叠氮化钠

产品应用

建议稀释浓度：1:25-1:100(for most application)

储存条件

-25 ~ -15℃分装保存，尽量避免反复冻融。

注意事项

1）为了您的安全和健康，请穿实验服并戴一次性手套操作；

2）本产品仅作科研用途！

Ver.CN20240228

暂无内容

重组FITC标记人FOLR1蛋白 Recombinant FITC-Labeled Human FOLR1 Protein,His-Avi Tag

发表于2024年3月6日由上海金畔生物科技有限公司

重组FITC标记人FOLR1蛋白 Recombinant FITC-Labeled Human FOLR1 Protein,His-Avi Tag

产品说明书

FAQ

COA

已发表文献

性能参数

分子别名（Synonyms）	FR-alpha; FR alpha; FR α; FOLR; FOLR1; FBP; Folbp1; KB cells FBP; MOv18; Folate receptor 1; Fbp1; Folate receptor alpha;
表达区间及表达系统（Source）	FITC-Labeled Human FOLR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg25-Met233.[Accession \| P15328]
分子量大小（Molecular Weight）	The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 37-47 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized FITC-Labeled Human FOLR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA.
制剂（Formulation）	Supplied as 0.22 μm filtered solution in PBS (pH 7.4).

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

性能参数

分子别名（Synonyms）	FR-alpha; FR alpha; FR α; FOLR; FOLR1; FBP; Folbp1; KB cells FBP; MOv18; Folate receptor 1; Fbp1; Folate receptor alpha;
表达区间及表达系统（Source）	FITC-Labeled Human FOLR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg25-Met233.[Accession \| P15328]
分子量大小（Molecular Weight）	The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 37-47 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized FITC-Labeled Human FOLR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA.
制剂（Formulation）	Supplied as 0.22 μm filtered solution in PBS (pH 7.4).

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC标记人FOLR1蛋白 Recombinant FITC-Labeled Human FOLR1 Protein,His-Avi Tag

暂无内容

重组FITC标记人NKG2D/CD314蛋白 Recombinant FITC-Labeled Human NKG2D/CD314 Protein,hFc-Flag Tag

发表于2024年3月6日由上海金畔生物科技有限公司

重组FITC标记人NKG2D/CD314蛋白 Recombinant FITC-Labeled Human NKG2D/CD314 Protein,hFc-Flag Tag

产品说明书

FAQ

COA

已发表文献

性能参数

分子别名（Synonyms）	CD314; D12S2489E; KLR; NKG2-D; NKG2D
表达区间及表达系统（Source）	FITC-Labeled Human NKG2D/CD314 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus. It contains Phe78-Val216.[Accession \| P26718]
分子量大小（Molecular Weight）	The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
制剂（Formulation）	Supplied as 0.22μm filtered solution in PBS (pH 7.4).

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

性能参数

分子别名（Synonyms）	CD314; D12S2489E; KLR; NKG2-D; NKG2D
表达区间及表达系统（Source）	FITC-Labeled Human NKG2D/CD314 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus. It contains Phe78-Val216.[Accession \| P26718]
分子量大小（Molecular Weight）	The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
制剂（Formulation）	Supplied as 0.22μm filtered solution in PBS (pH 7.4).

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC标记人NKG2D/CD314蛋白 Recombinant FITC-Labeled Human NKG2D/CD314 Protein,hFc-Flag Tag

暂无内容

重组FITC标记人VEGF165蛋白 Recombinant FITC-Labeled Human VEGF165 Protein,His-Avi Tag

发表于2024年3月6日由上海金畔生物科技有限公司

重组FITC标记人VEGF165蛋白 Recombinant FITC-Labeled Human VEGF165 Protein,His-Avi Tag

产品说明书

FAQ

COA

已发表文献

性能参数

分子别名（Synonyms）	VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165
表达区间及表达系统（Source）	FITC-Labeled Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala27-Arg191.[Accession \| P15692-4]
分子量大小（Molecular Weight）	The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-33 kDa under reduced (R) condition, 45-55 kDa under Non reducing (N) condition based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized FITC-Labeled Human VEGF165, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 22.3ng/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

性能参数

分子别名（Synonyms）	VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165
表达区间及表达系统（Source）	FITC-Labeled Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala27-Arg191.[Accession \| P15692-4]
分子量大小（Molecular Weight）	The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-33 kDa under reduced (R) condition, 45-55 kDa under Non reducing (N) condition based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized FITC-Labeled Human VEGF165, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 22.3ng/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC标记人Her3/ErbB3蛋白 Recombinant FITC-Labeled Human Her3/ErbB3 Protein,His-Avi Tag

发表于2024年3月6日由上海金畔生物科技有限公司

重组FITC标记人Her3/ErbB3蛋白 Recombinant FITC-Labeled Human Her3/ErbB3 Protein,His-Avi Tag

产品说明书

FAQ

COA

已发表文献

性能参数

分子别名（Synonyms）	ErbB3; ErbB-3; HER3; HER3c-erbB-3; LCCS2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; ERBB3
表达区间及表达系统（Source）	FITC-Labeled Human Her3/ErbB3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser20-Thr643.[Accession \| P21860-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 71.6 kDa. Due to glycosylation, the protein migrates to 72-75 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in HER-HM403F. Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

性能参数

分子别名（Synonyms）	ErbB3; ErbB-3; HER3; HER3c-erbB-3; LCCS2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; ERBB3
表达区间及表达系统（Source）	FITC-Labeled Human Her3/ErbB3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser20-Thr643.[Accession \| P21860-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 71.6 kDa. Due to glycosylation, the protein migrates to 72-75 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in HER-HM403F. Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC 兼容人BCMA/TNFRSF17蛋白 Recombinant FITC-Compatible Human BCMA/TNFRSF17 Protein,His Tag

发表于2024年3月6日由上海金畔生物科技有限公司

重组FITC 兼容人BCMA/TNFRSF17蛋白 Recombinant FITC-Compatible Human BCMA/TNFRSF17 Protein,His Tag

产品说明书

FAQ

COA

已发表文献

性能参数

分子别名（Synonyms）	BCMA;TNFRSF13A;TNFRSF17;CD269
表达区间及表达系统（Source）	FITC-Compatible Human BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Met1-Ala54.[Accession \| Q02223-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 35.9 kDa. Due to glycosylation, the protein migrates to 37-48 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
制剂（Formulation）	Supplied as 0.22μm filtered solution in PBS (pH 7.4).

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

性能参数

分子别名（Synonyms）	BCMA;TNFRSF13A;TNFRSF17;CD269
表达区间及表达系统（Source）	FITC-Compatible Human BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Met1-Ala54.[Accession \| Q02223-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 35.9 kDa. Due to glycosylation, the protein migrates to 37-48 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
制剂（Formulation）	Supplied as 0.22μm filtered solution in PBS (pH 7.4).

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC 兼容人BCMA/TNFRSF17蛋白 Recombinant FITC-Compatible Human BCMA/TNFRSF17 Protein,His Tag

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