ACROBiosystems CDA-H82E7-25ug 说明书

发表于2024年4月18日由上海金畔生物科技有限公司

世界*实验材料供应商 ACROBiosystems 上海金畔生物为其中国代理， ACROBiosystems 在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， ACROBiosystems 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

ACROBiosystems 中国代理, ACROBiosystems 上海代理， ACROBiosystems 北京代理，ACROBiosystems 广东代理， ACROBiosystems 江苏代理ACROBiosystems 湖北代理,ACROBiosystems 天津,ACROBiosystems 黑龙江代理,ACROBiosystems 内蒙古代理,ACROBiosystems 吉林代理,ACROBiosystems 福建代理, ACROBiosystems 江苏代理, ACROBiosystems 浙江代理, ACROBiosystems 四川代理,

ACROBiosystems 公司是一个具有范围影响力的生物技术，其运营包括了位于北美、欧洲和亚洲的数家公司，主要为生命科学研究、生物制药与疫病诊断等行业提供产品、技术服务和临床应用等解决方案，同时致力于在蛋白组学、药物发现和细胞治疗等领域推广面向工业化和临床转化的技术和产品。ACROBiosystems 的业务遍及60多个国家和地区，并通过在中国、英国等世界范围的不同时区设立分支机构以服务于当地客户与经销商

Biotinylated Human Fc gamma RIIA / CD32a (R167), Avi Tag (Avitag™)

物素化的人FcγRIIA/ CD32a（R167），Avi标签（Avitag TM）

货号

CDA-H82E7-25ug

CDA-H82E7-200ug

代名词

CD32a，FCGR2A，CD32，FCG2，FCGR2A1，IGFR2

资源

从人HEK293细胞表达生物素化的人FcγRIIA/ CD32a（R167）（CDA-H82E7）。它含有AA Ala 36 – Ile 218（登记号P12318-1）。

预测的N-末端：Ala 36

分子表征

该蛋白在C-末端带有Avi标签（Avitag TM），随后是聚组氨酸标签。

该蛋白质具有24kDa的计算MW。由于糖基化，蛋白质在还原（R）条件下（SDS-PAGE）迁移为30-38kDa。

生物素

该产品的生物素化使用Avitag™技术进行。简言之，Avitag中的单个赖氨酸残基用生物素酶促标记。

生物素：蛋白质比例

通过HABA测定确定的生物素与蛋白质的比例是0.5-1。

内毒素

LAL方法每μg不足1.0 EU。

纯度

通过SDS-PAGE测定> 95％。

公式

从0.22μm过滤的PBS溶液（pH7.4）中冻干。通常在冻干前加入海藻糖作为保护剂。

定制产品形式或配方。

重建

请参阅分析证书了解具体说明。

为获得*性能，我们强烈建议您遵循CoA提供的重组协议。

存储

对于长期储存，产品应在-20°C或更低的温度下保存在冻干状态。

请避免反复的冻融循环。

储存后没有观察到活性损失：

4-8 ^° C冻干12个月;
-70 ^° C在无菌条件下重建3个月。

重组生物素化人SIRPα/CD172a蛋白(hFc-Avi标签) Human SIRP alpha/CD172a Protein

发表于2024年3月4日由上海金畔生物科技有限公司

重组生物素化人SIRPα/CD172a蛋白(hFc-Avi标签) Human SIRP alpha/CD172a Protein

产品说明书

FAQ

COA

已发表文献

性能参数

分子别名（Synonyms）	MFR; BIT;CD172a; MYD-1
表达区间及表达系统（Source）	Biotinylated Human SIRP alpha/CD172a Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Glu31-Arg370.[Accession \| P78324-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 66.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Human CD47, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human SIRP alpha, hFc Tag with the EC50 of 0.22μg/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

性能参数

分子别名（Synonyms）	MFR; BIT;CD172a; MYD-1
表达区间及表达系统（Source）	Biotinylated Human SIRP alpha/CD172a Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Glu31-Arg370.[Accession \| P78324-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 66.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Human CD47, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human SIRP alpha, hFc Tag with the EC50 of 0.22μg/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组生物素化人SIRPα/CD172a蛋白(hFc-Avi标签) Human SIRP alpha/CD172a Protein

暂无内容

重组小鼠SIRPα/CD172a蛋白(His标签) Mouse SIRP alpha/CD172a Protein

发表于2024年3月4日由上海金畔生物科技有限公司

重组小鼠SIRPα/CD172a蛋白(His标签) Mouse SIRP alpha/CD172a Protein

产品说明书

FAQ

COA

已发表文献

性能参数

分子别名（Synonyms）	MFR; BIT;CD172a; MYD-1
表达区间及表达系统（Source）	Mouse SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Lys32-Asn373.[Accession \| BAA13520.1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Mouse SIRP alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26μg/ml determined by ELISA .
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

性能参数

分子别名（Synonyms）	MFR; BIT;CD172a; MYD-1
表达区间及表达系统（Source）	Mouse SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Lys32-Asn373.[Accession \| BAA13520.1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Mouse SIRP alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26μg/ml determined by ELISA .
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组生物素化人FcγRIIIA/CD16a（F176）蛋白(His-Avi标签) Human Fc gamma RIIIA/CD16a(F176) Protein

发表于2024年3月1日由上海金畔生物科技有限公司

重组生物素化人FcγRIIIA/CD16a（F176）蛋白(His-Avi标签) Human Fc gamma RIIIA/CD16a(F176) Protein

产品说明书

FAQ

COA

已发表文献

性能参数

表达区间及表达系统（Source）	Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.. It containsGly17-Gln208(F176).Uniprot \| P08637-1
分子量大小（Molecular Weight）	The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by Tris-Bis PAGE and HPLC
活性（Activity）	ELISA Data: Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA SPR Data:Biotinylated Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14μM as determined in SPR assay
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

Ver. CN20230602

性能参数

表达区间及表达系统（Source）	Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.. It containsGly17-Gln208(F176).Uniprot \| P08637-1
分子量大小（Molecular Weight）	The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by Tris-Bis PAGE and HPLC
活性（Activity）	ELISA Data: Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA SPR Data:Biotinylated Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14μM as determined in SPR assay
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

Ver. CN20230602

重组生物素化人FcγRIIIA/CD16a（F176）蛋白(His-Avi标签) Human Fc gamma RIIIA/CD16a(F176) Protein

暂无内容

重组生物素化人FcγRIIA/CD32a(H167)蛋白(His-Avi标签) Human Fc gamma RIIA/CD32a (H167) Protein

发表于2024年3月1日由上海金畔生物科技有限公司

重组生物素化人FcγRIIA/CD32a(H167)蛋白(His-Avi标签) Human Fc gamma RIIA/CD32a (H167) Protein

产品说明书

FAQ

COA

已发表文献

性能参数

表达区间及表达系统（Source）	Recombinant Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218(H167).[Uniprot \| P12318-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by Tris-Bis PAGE and HPLC
活性（Activity）	SPR Data: Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 1.44 μM as determined in SPR assay . SPR Data: Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.680 μM as determined in SPR assay
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

Ver. CN20230421

性能参数

表达区间及表达系统（Source）	Recombinant Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218(H167).[Uniprot \| P12318-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by Tris-Bis PAGE and HPLC
活性（Activity）	SPR Data: Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 1.44 μM as determined in SPR assay . SPR Data: Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.680 μM as determined in SPR assay
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

Ver. CN20230421

重组生物素化人FcγRIIA/CD32a(H167)蛋白(His-Avi标签) Human Fc gamma RIIA/CD32a (H167) Protein

暂无内容

重组人Fc γ RIIA/CD32a(R167)(His-Avi标签)免疫蛋白

发表于2024年3月1日由上海金畔生物科技有限公司

重组人Fc γ RIIA/CD32a(R167)(His-Avi标签)免疫蛋白

产品说明书

FAQ

COA

已发表文献

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16). Three genes for human Fc gamma RII (A, B, and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc gamma RII A and C) or ITIM motifs (Fc gamma RIIB) in their cytoplasmic domains, have been identified. The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

产品性质

别名	Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A
Uniprot No.	P12318.1
表达区间及表达系统	Recombinant Human Fc gamma RIIA/CD32a (R167) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218.
分子量	Approximately 23.2 kDa. Due to glycosylation, the protein migrates to 34-40 kDa based on Tris-Bis PAGE result.
纯度	> 95% as determined by SDS-PAGE and HPLC.
活性	SPR Data：Human Fc gamma RIIA (R167) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 9μM as determined in SPR assay (Biacore T200).
内毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制剂	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

产品数据

HB220810

产品性质

别名	Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A
Uniprot No.	P12318.1
表达区间及表达系统	Recombinant Human Fc gamma RIIA/CD32a (R167) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218.
分子量	Approximately 23.2 kDa. Due to glycosylation, the protein migrates to 34-40 kDa based on Tris-Bis PAGE result.
纯度	> 95% as determined by SDS-PAGE and HPLC.
活性	SPR Data：Human Fc gamma RIIA (R167) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 9μM as determined in SPR assay (Biacore T200).
内毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制剂	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

产品数据

HB220810

暂无内容

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

发表于2024年3月1日由上海金畔生物科技有限公司

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

产品说明书

FAQ

COA

已发表文献

Fc gamma RIIIA/CD16a is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes. In humans, a single nucleotide polymorphism (T230A) creates high binding (176V) and low binding (176F) forms that may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies. Fc gamma RIIIA surface expression requires interaction with an accessory chain, either the common gamma -chain or CD3 zeta. Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA. Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity. Shedding from monocytes and macrophages can be triggered by cell activation or phagocytosis. Soluble Fc gamma RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases.

产品性质

别名	Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20
Accession	AAH17865
表达区间及表达系统	Recombinant Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly17-Gln208.
分子量	Approximately 24.7 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.
纯度	> 95% as determined by SDS-PAGE and HPLC.
活性	ELISA Data：Immobilized Human Fc gamma RIIIA (V176) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 0.09μg/ml determined by ELISA. SPR Data：Human Fc gamma RIIIA (V176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 67.3nM as determined in SPR assay (Biacore T200).
内毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制剂	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

产品数据

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

HB220811

产品性质

别名	Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20
Accession	AAH17865
表达区间及表达系统	Recombinant Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly17-Gln208.
分子量	Approximately 24.7 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.
纯度	> 95% as determined by SDS-PAGE and HPLC.
活性	ELISA Data：Immobilized Human Fc gamma RIIIA (V176) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 0.09μg/ml determined by ELISA. SPR Data：Human Fc gamma RIIIA (V176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 67.3nM as determined in SPR assay (Biacore T200).
内毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制剂	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

产品数据

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

HB220811

暂无内容

重组人FcγRIIA/CD32a(H167)(His-Avi标签)|Recombinant Human Fc gamma RIIA/CD32a

发表于2024年3月1日由上海金畔生物科技有限公司

重组人FcγRIIA/CD32a(H167)(His-Avi标签)|Recombinant Human Fc gamma RIIA/CD32a

产品说明书

FAQ

COA

已发表文献

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG, both Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC).The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands.

产品性质

别名（Synonyms）	Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A
Uniprot No.	P12318-1
表达区间及表达系统（Source）	Recombinant Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218(H167).
分子量(Molecular Weight)	The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
外观(Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC
活性（Activity）	SPR Data：Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.606 μM as determined in SPR assay.
内毒素（Endotoxin）	< 1.0 EU per 1μg of the protein by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

产品数据

重组人FcγRIIA/CD32a(H167)(His-Avi标签)|Recombinant Human Fc gamma RIIA/CD32a

HB220624

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG, both Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC).The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands.

产品性质

别名（Synonyms）	Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A
Uniprot No.	P12318-1
表达区间及表达系统（Source）	Recombinant Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218(H167).
分子量(Molecular Weight)	The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
外观(Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC
活性（Activity）	SPR Data：Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.606 μM as determined in SPR assay.
内毒素（Endotoxin）	< 1.0 EU per 1μg of the protein by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

产品数据

重组人FcγRIIA/CD32a(H167)(His-Avi标签)|Recombinant Human Fc gamma RIIA/CD32a

HB220624

暂无内容

重组人FcγRIIIA/CD16a(F176)(His-Avi标签)|Recombinant Human Fc gamma RIIIA/CD16a

发表于2024年2月29日由上海金畔生物科技有限公司

重组人FcγRIIIA/CD16a(F176)(His-Avi标签)|Recombinant Human Fc gamma RIIIA/CD16a

产品说明书

FAQ

COA

已发表文献

Fc gamma RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes. In humans, it is a 50-70 kDa type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIb is highly related, sharing 97% amino acid (aa) identity within the extracellular domain (ECD), but is a GPI-linked receptor expressed on human neutrophils and eosinophils. The ECD of Fc gamma RIIIa shares 63%, 61%, 65%, 59% and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIa, feline CD16, bovine CD16 and porcine Fc gamma RIIIb paralogs, respectively. The Fc gamma RIIIa cDNA encodes 254 aa including a 16 aa signal sequence, 191 aa ECD with two C2-type Ig-like domains and five potential N-glycosylation sites, a 22 aa transmembrane (TM) sequence and a 25 aa cytoplasmic domain.

产品性质

别名（Synonyms）	Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20
Uniprot No.	P08637-1
表达区间及表达系统（Source）	Recombinant Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal.. It contains Gly17-Gln208(F176).
分子量(Molecular Weight)	The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
外观(Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC
活性（Activity）	ELISA Data：Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC₅₀ of 9.5ng/ml determined by ELISA. SPR Data: Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 0.92μM as determined in SPR assay.
内毒素（Endotoxin）	< 1.0 EU per 1μg of the protein by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

产品数据

重组人FcγRIIIA/CD16a(F176)(His-Avi标签)|Recombinant Human Fc gamma RIIIA/CD16a

HB220624

产品性质

别名（Synonyms）	Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20
Uniprot No.	P08637-1
表达区间及表达系统（Source）	Recombinant Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal.. It contains Gly17-Gln208(F176).
分子量(Molecular Weight)	The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
外观(Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC
活性（Activity）	ELISA Data：Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC₅₀ of 9.5ng/ml determined by ELISA. SPR Data: Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 0.92μM as determined in SPR assay.
内毒素（Endotoxin）	< 1.0 EU per 1μg of the protein by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

产品数据

重组人FcγRIIIA/CD16a(F176)(His-Avi标签)|Recombinant Human Fc gamma RIIIA/CD16a

HB220624

暂无内容