重组小鼠SIRPα/CD172a蛋白(His标签) Mouse SIRP alpha/CD172a Protein

重组小鼠SIRPα/CD172a蛋白(His标签) Mouse SIRP alpha/CD172a Protein

产品说明书

FAQ

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已发表文献

 

性能参数

分子别名(Synonyms)

MFR; BIT;CD172a; MYD-1

表达区间及表达系统(Source)

Mouse SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Lys32-Asn373.[Accession | BAA13520.1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Mouse SIRP alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26μg/ml determined by ELISA .

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名(Synonyms)

MFR; BIT;CD172a; MYD-1

表达区间及表达系统(Source)

Mouse SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Lys32-Asn373.[Accession | BAA13520.1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Mouse SIRP alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26μg/ml determined by ELISA .

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.