产品描述

本品是由Alexa Fluor 594标记的山羊抗兔IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。Alexa Fluor 594的光谱性质类似于Texas Red，Amax（最大激发波长）为591nm，Emax（最大发射波长）为614nm。

本品适合做单标实验，广泛应用于多种免疫学实验如免疫细胞化学（ICC），流式细胞术（FC）以及免疫组化（IHC）等。要做多标（multiple-labeling）实验，建议使用与相近物种的血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

产品应用

建议稀释浓度：1:50-1:400（For most application）

产品性质 

运输与保存方法

冰袋运输。-20℃分装保存，尽量避免反复冻融。有效期1年。

注意事项

1）本品含叠氮化钠，对人体有害，请注意适当防护。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

3）本产品仅作科研用途！

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[2] Sun X, He X, Zhang Y, et al. Inflammatory cell-derived CXCL3 promotes pancreatic cancer metastasis through a novel myofibroblast-hijacked cancer escape mechanism. Gut. 2022;71(1):129-147. doi:10.1136/gutjnl-2020-322744(IF:23.059)
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[14] Yang S, Yang Y, Chen C, et al. The Anti-Neuroinflammatory Effect of Fuzi and Ganjiang Extraction on LPS-Induced BV2 Microglia and Its Intervention Function on Depression-Like Behavior of Cancer-Related Fatigue Model Mice. Front Pharmacol. 2021;12:670586. Published 2021 May 28. doi:10.3389/fphar.2021.670586(IF:5.811)
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[16] Li C, Liu F, Liu S, et al. Elevated myocardial SORBS2 and the underlying implications in left ventricular noncompaction cardiomyopathy. EBioMedicine. 2020;53:102695. doi:10.1016/j.ebiom.2020.102695(IF:5.736)
[17] Li C, Zheng Y, Liu Y, et al. The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease. Aging (Albany NY). 2022;14(2):800-810. doi:10.18632/aging.203841(IF:5.682)
[18] Xia C, Qin X, Zhou L, et al. Reproductive Regulation of PrRPs in Teleost: The Link Between Feeding and Reproduction. Front Endocrinol (Lausanne). 2021;12:762826. Published 2021 Nov 3. doi:10.3389/fendo.2021.762826(IF:5.555)
[19] Wu X, Qian S, Zhang J, et al. Lipopolysaccharide promotes metastasis via acceleration of glycolysis by the nuclear factor-κB/snail/hexokinase3 signaling axis in colorectal cancer. Cancer Metab. 2021;9(1):23. Published 2021 May 12. doi:10.1186/s40170-021-00260-x(IF:5.469)
[20] Zhang X, Zhao D, Wu W, et al. Melatonin regulates mitochondrial dynamics and alleviates neuron damage in prion diseases. Aging (Albany NY). 2020;12(11):11139-11151. doi:10.18632/aging.103328(IF:4.831)
[21] Yao H, Xu K, Zhou J, Zhou L, Wei S. A Tumor Microenvironment Destroyer for Efficient Cancer Suppression. ACS Biomater Sci Eng. 2020;6(1):450-462. doi:10.1021/acsbiomaterials.9b01544(IF:4.511)
[22] Wang LJ, He L, Hao L, et al. Isoliquiritigenin ameliorates caerulein-induced chronic pancreatitis by inhibiting the activation of PSCs and pancreatic infiltration of macrophages. J Cell Mol Med. 2020;24(17):9667-9681. doi:10.1111/jcmm.15498(IF:4.486)
[23] Li B, Wu J, Bao J, et al. Activation of α7nACh receptor protects against acute pancreatitis through enhancing TFEB-regulated autophagy. Biochim Biophys Acta Mol Basis Dis. 2020;1866(12):165971. doi:10.1016/j.bbadis.2020.165971(IF:4.352)
[24] Yao D, He Q, Sun J, et al. FGF21 attenuates hypoxia‑induced dysfunction and inflammation in HPAECs via the microRNA‑27b‑mediated PPARγ pathway. Int J Mol Med. 2021;47(6):116. doi:10.3892/ijmm.2021.4949(IF:4.101)
[25] Yao L, Li Y, Diao Z, et al. Stress Controllability Modulates Basal Activity of Dopamine Neurons in the Substantia Nigra Compacta. eNeuro. 2021;8(3):ENEURO.0044-21.2021. Published 2021 Jun 16. doi:10.1523/ENEURO.0044-21.2021(IF:4.081)
[26] Yang S, Chu S, Ai Q, et al. Anti-inflammatory effects of higenamine (Hig) on LPS-activated mouse microglia (BV2) through NF-κB and Nrf2/HO-1 signaling pathways. Int Immunopharmacol. 2020;85:106629. doi:10.1016/j.intimp.2020.106629(IF:3.943)
[27] Yang X, Zou R, Dai X, Wu X, Yuan F, Feng Y. YAP is critical to inflammation, endothelial-mesenchymal transition and subretinal fibrosis in experimental choroidal neovascularization. Exp Cell Res. 2022;417(2):113221. doi:10.1016/j.yexcr.2022.113221(IF:3.905)
[28] Li C, Zhang H, Xie Y, et al. Effects of CMYA1 overexpression on cardiac structure and function in mice. Acta Biochim Biophys Sin (Shanghai). 2021;53(5):593-600. doi:10.1093/abbs/gmab029(IF:3.848)
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[31] Peng C, Zhang W, Dai C, et al. Study of the aqueous extract of Aloe vera and its two active components on the Wnt/β-catenin and Notch signaling pathways in colorectal cancer cells. J Ethnopharmacol. 2019;243:112092. doi:10.1016/j.jep.2019.112092(IF:3.414)
[32] Li Q , Sun J , Mohammadtursun N , Wu J , Dong J , Li L . Curcumin inhibits cigarette smoke-induced inflammation via modulating the PPARγ-NF-κB signaling pathway. Food Funct. 2019;10(12):7983-7994. doi:10.1039/c9fo02159k(IF:3.241)
[33] Lin J, Yu XQ, Wang Q, et al. Immune responses to Bacillus thuringiensis in the midgut of the diamondback moth, Plutella xylostella. Dev Comp Immunol. 2020;107:103661. doi:10.1016/j.dci.2020.103661(IF:3.192)
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产品描述

本品是由Alexa Fluor 647标记的山羊抗兔IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。

Alexa Fluor 647的光谱性质类似于APC，其最大激发波长（Amax）为651 nm，最大发射波长（Emax）为667 nm。本品适合做单标实验。要做多标（multiple-labeling）实验，建议使用与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

产品应用

建议稀释浓度：1:50-1:400（For most applications）

产品性质

运输与保存方法

冰袋运输。-20℃分装保存，尽量避免反复冻融。有效期1年。
 

注意事项

1）本品含叠氮化钠，对人体有害，请注意适当防护。
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
3）本产品仅作科研用途！

[1] Zhou Y, Ke P, Bao X, et al. Peptide nano-blanket impedes fibroblasts activation and subsequent formation of pre-metastatic niche. Nat Commun. 2022;13(1):2906. Published 2022 May 25. doi:10.1038/s41467-022-30634-8(IF:14.919)
[2] Jin ZR, Fang D, Liu BH, et al. Roles of CatSper channels in the pathogenesis of asthenozoospermia and the therapeutic effects of acupuncture-like treatment on asthenozoospermia. Theranostics. 2021;11(6):2822-2844. Published 2021 Jan 1. doi:10.7150/thno.51869(IF:11.556)
[3] Zhang B, Wang H, Liao Z, et al. EGFP-EGF1-conjugated nanoparticles for targeting both neovascular and glioma cells in therapy of brain glioma. Biomaterials. 2014;35(13):4133-4145. doi:10.1016/j.biomaterials.2014.01.071(IF:8.312)
[4] Luo Z, Jin K, Pang Q, et al. On-Demand Drug Release from Dual-Targeting Small Nanoparticles Triggered by High-Intensity Focused Ultrasound Enhanced Glioblastoma-Targeting Therapy. ACS Appl Mater Interfaces. 2017;9(37):31612-31625. doi:10.1021/acsami.7b10866(IF:7.504)
[5] Wang C, Zhang J, Song S, et al. Imaging epileptic foci in mouse models via a low-density lipoprotein receptor-related protein-1 targeting strategy. EBioMedicine. 2021;63:103156. doi:10.1016/j.ebiom.2020.103156(IF:5.736)
[6] Wang L, Qian Y, Che X, Jiang J, Suo J, Wang Z. Isolation and Characterization of Primary Retinal Microglia From the Human Post-mortem Eyes for Future Studies of Ocular Diseases. Front Cell Neurosci. 2022;15:786020. Published 2022 Jan 13. doi:10.3389/fncel.2021.786020(IF:5.505)
[7] Zhao J, Xie W, Yang Z, et al. Identification and characterization of a special type of subnuclear structure: AGGF1-coated paraspeckles. FASEB J. 2022;36(6):e22366. doi:10.1096/fj.202101690RR(IF:5.192)

产品简介

本品是由Alexa Fluor® 488标记的山羊抗兔IgG(H+L) F(ab') ₂，是将山羊抗血清经胃蛋白酶消化并使用抗原偶联的琼脂糖微珠亲和色谱纯化所得，借此可去除Fc片段和完整IgG分子。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。本品预先与相近物种包括人、小鼠、大鼠的血清蛋白经过亲和吸附处理，ELISA和/或固相免疫吸附检测证实本品与以上物种几乎无交叉反应。但可能会与其他物种免疫球蛋白发生交叉反应。本品不会与非免疫球蛋白类的血清蛋白发生交叉反应。

Alexa Fluor® 488的光谱性质同FITC，Amax(最大激发波长)为493 nm，Emax(最大发射波长)为519 nm。本品适合做单标实验，广泛应用于多种免疫学实验如免疫细胞化学（ICC），流式细胞术（FC）等。要做多标（Multiple-labeling）实验，建议使用经过与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

产品应用

建议稀释浓度：1:100-1:800（For most application）

产品信息

储存条件

-25 ~ -15℃分装保存，尽量避免反复冻融。有效期1年。

注意事项

1.本品含叠氮化钠，对人体有害，请注意适当防护。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

[1] He XL, Yang L, Wang ZJ, Huang RQ, Zhu RR, Cheng LM. Solid lipid nanoparticles loading with curcumin and dexanabinol to treat major depressive disorder. Neural Regen Res. 2021;16(3):537-542. doi:10.4103/1673-5374.293155(IF:5.135)
[2] Wei W, Zhang Y, Zhang D, et al. Annotating BCMA Expression in Multiple Myelomas [published online ahead of print, 2021 Nov 29]. Mol Pharm. 2021;10.1021/acs.molpharmaceut.1c00628. doi:10.1021/acs.molpharmaceut.1c00628(IF:4.939)
[3] Li T, Bai J, Du Y, et al. Thiamine pretreatment improves endotoxemia-related liver injury and cholestatic complications by regulating galactose metabolism and inhibiting macrophage activation. Int Immunopharmacol. 2022;108:108892. doi:10.1016/j.intimp.2022.108892(IF:4.932)
[4] Yang X, Zou R, Dai X, Wu X, Yuan F, Feng Y. YAP is critical to inflammation, endothelial-mesenchymal transition and subretinal fibrosis in experimental choroidal neovascularization. Exp Cell Res. 2022;417(2):113221. doi:10.1016/j.yexcr.2022.113221(IF:3.905)