DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated)
一键复制产品信息 产品编号: AF2909
产品包装:50μl
选择包装
50μl
数
量
DsRed Tag Mouse mAb (HRP Conjugated)(DsRed Tag 小鼠单抗)(AF2909)
一键复制产品信息 说明书下载
价格: ¥ 388.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2909 | DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl | 388.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IHC, ICC | DsRed-tagged protein in all species | – | – |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | – | – | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) |
| Category | Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | – |
| Cellular location | – |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Recombinant DsRed protein expressed in E. coli |
| Sequence | – |
| Gene ID | – |
| SwissProt | – |
| Synonyms | – |
| Category | Polypeptide protein tag |
| Background | DsRed is a red fluorescent protein cloned by homology to Green Fluorescent Protein (GFP) from Discosoma coral. DsRed exhibits excellent resistance to pH extremes and photobleaching and causes a strong red-shift from 558 nm to 583 nm once matured. Immature DsRed shows GFP-like excitation and emission maxima. The contrast created through GFP and DsRed spectral shifts allows for a powerful dual reporter system. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2909 | DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
保存条件：
DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作，无须再孵育二抗。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作，无须再孵育二抗。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。
相关产品：
| 产品编号 | 产品名称 | 包装 |
| AF2852 | Flag Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2855 | Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2858 | HA Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2861 | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2864 | Myc Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2867 | Myc Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2870 | His Tag (C-terminal Specific) Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2873 | His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2876 | His Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2879 | His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2882 | GFP Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2885 | GFP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2888 | GST Tag Mouse Monoclonal Antibody | 50μl/200μl |
| AF2891 | GST Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
| AF2894 | V5 Tag Mouse Monoclonal Antibody | 50μl/200μl |
| AF2897 | V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
| AF2900 | Glu-Glu Tag Mouse Monoclonal Antibody | 50μl |
| AF2906 | DsRed Tag Mouse Monoclonal Antibody | 50μl |
| AF2909 | DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2912 | MBP Tag Mouse Monoclonal Antibody | 50μl |
| AF2915 | MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2918 | PA Tag Mouse Monoclonal Antibody | 50μl |
| AF2921 | PA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2924 | StrepII Tag Mouse Monoclonal Antibody | 50μl |
| AF2927 | StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2930 | S Tag Mouse Monoclonal Antibody | 50μl |
| AF2933 | S Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2936 | TEV-His Tag Mouse Monoclonal Antibody | 50μl |
| AF2939 | TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2942 | Target Tag Mouse Monoclonal Antibody | 50μl |
| AF2945 | Target Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2948 | TRX Tag Mouse Monoclonal Antibody | 50μl |
| AF2951 | TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2954 | AVI Tag Mouse Monoclonal Antibody | 50μl |
| AF2957 | AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
质检证书
输入批号搜寻COA
搜索
相关产品请点击如下按钮：
标签抗体
HRP标记一抗
使用本产品的文献：
DsRed Tag Mouse mAb(DsRed Tag 小鼠单抗)(AF2906)
DsRed Tag Mouse Monoclonal Antibody
一键复制产品信息 产品编号: AF2906
产品包装:50μl
选择包装
50μl
数
量
说明书下载
价格: ¥ 299.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2906 | DsRed Tag Mouse Monoclonal Antibody | 50μl | 299.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IP, IF, IHC, ICC | DsRed-tagged protein in all species | – | – |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | 1:50 | 1:50-1:100 | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | DsRed Tag Mouse Monoclonal Antibody |
| Category | Mouse Monoclonal Antibody (mAb); Primary antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | – |
| Cellular location | – |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Recombinant DsRed protein expressed in E. coli |
| Sequence | – |
| Gene ID | – |
| SwissProt | – |
| Synonyms | – |
| Category | Polypeptide protein tag |
| Background | DsRed is a red fluorescent protein cloned by homology to Green Fluorescent Protein (GFP) from Discosoma coral. DsRed exhibits excellent resistance to pH extremes and photobleaching and causes a strong red-shift from 558 nm to 583 nm once matured. Immature DsRed shows GFP-like excitation and emission maxima. The contrast created through GFP and DsRed spectral shifts allows for a powerful dual reporter system. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2906 | DsRed Tag Mouse Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
保存条件：
DsRed Tag Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
| 产品编号 | 产品名称 | 包装 |
| AF2852 | Flag Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2855 | Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2858 | HA Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2861 | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2864 | Myc Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2867 | Myc Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2870 | His Tag (C-terminal Specific) Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2873 | His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2876 | His Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2879 | His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2882 | GFP Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2885 | GFP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2888 | GST Tag Mouse Monoclonal Antibody | 50μl/200μl |
| AF2891 | GST Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
| AF2894 | V5 Tag Mouse Monoclonal Antibody | 50μl/200μl |
| AF2897 | V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
| AF2900 | Glu-Glu Tag Mouse Monoclonal Antibody | 50μl |
| AF2906 | DsRed Tag Mouse Monoclonal Antibody | 50μl |
| AF2909 | DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2912 | MBP Tag Mouse Monoclonal Antibody | 50μl |
| AF2915 | MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2918 | PA Tag Mouse Monoclonal Antibody | 50μl |
| AF2921 | PA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2924 | StrepII Tag Mouse Monoclonal Antibody | 50μl |
| AF2927 | StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2930 | S Tag Mouse Monoclonal Antibody | 50μl |
| AF2933 | S Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2936 | TEV-His Tag Mouse Monoclonal Antibody | 50μl |
| AF2939 | TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2942 | Target Tag Mouse Monoclonal Antibody | 50μl |
| AF2945 | Target Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2948 | TRX Tag Mouse Monoclonal Antibody | 50μl |
| AF2951 | TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2954 | AVI Tag Mouse Monoclonal Antibody | 50μl |
| AF2957 | AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
质检证书
输入批号搜寻COA
搜索
相关产品请点击如下按钮：
标签抗体
使用本产品的文献：
RNAi-Ready pSIREN-Retro Q-DsRed-Express
RNAi载体，RNAi-Ready pSIREN-Retro Q-DsRed-Express载体
RNAi-Ready pSIREN-Retro Q-DsRed-Express
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VRC0346 | RNAi-Ready pSIREN-Retro Q-DsRed-Express | Clontech | RNAi载体 |
载体描述:
RNAi-Ready pSIREN-RetroQ-DsRed-Express is a self-inactivating retroviral expression vector designed to express a small hairpin RNA （shRNA） using the human U6 promoter （PU6; RNA Pol III-dependent）. RNAi-Ready pSIREN-RetroQ-DsRed-Express is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when a ds DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. You can transfect your pSIREN-RetroQDsRed-Express construct as a plasmid expression vector, or—upon transfection into a packaging cell line—this vector can transiently express, or integrate and stably express a viral genomic transcript containing the human U6 promoter and the shRNA.
质粒图谱:
pLVX-DsRed-Monomer-N1载体
pLVX-DsRed-Monomer-N1载体
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VMC0379 | pLVX-DsRed-Monomer-N1 | Clontech | 慢病毒载体 |
Description
pLVX-DsRed-Monomer-N1 is an HIV-1-based, lentiviral expression vector that allows you
to express your gene of interest fused to DsRed-Monomer, a monomeric mutant of the
Discosoma sp. red fluorescent protein. Genes cloned into the multiple cloning site （MCS）,
located upstream of the DsRed-Monomer coding sequence, are expressed as N-terminal
fusions of the DsRed-Monomer protein. Expression of the fusion protein is driven by the
constitutively active human cytomegalovirus immediate early promoter （P
CMV IE） located
just upstream of the MCS. Lentiviral particles derived from the vector allow the expression
of DsRed-Monomer fusion proteins in virtually any cell type, including primary cells. The
unmodified vector expresses DsRed-Monomer, and may be used to produce marker virus to
optimize infection protocols.
质粒图谱: