Tissue-Tek组织包埋盒


Tissue-Tek组织包埋盒

简要描述:Tissue-Tek® Process/Embedding Cassette Made from chemical resistant plastic.

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包埋耗材, Capsules, 模具, 和记号笔
 
Tissue Tek®; Sakura Finetek包埋模具
1. Tissue-Tek® Process/Embedding Cassette
Made from chemical resistant plastic. These cassettes have an angled, texture writing surface, ensuring positive identification throughout processing, embedding, sectioning and storage. Stainless steel cover is available separay
Cat. No.
Tissue-Tek
标签
Pack
Price
 
62520-C
4191
透明
1500/
3519
Add to Cart
62520-W
4187
白色
1500/
3502
Add to Cart
62520-A
4129
浅绿
1500/
3876
Add to Cart
62520-B
4184
蓝色
1500/
3876
Add to Cart
62520-G
4128
金色
1500/
3910
Add to Cart
62520-GR
4183
绿色
1500/
3910
Add to Cart
62520-O
4126
橙色
1500/
3910
Add to Cart
62520-P
4182
粉红
1500/
3910
Add to Cart
62520-Y
4179
黄色
1500/
3910
Add to Cart
62520-25
4194
SS Cassette Cover
25/
731
Add to Cart
2. Tissue-Tek® Embedding Rings
Made from polystyrene and reinforced with the dual bar center support to prevent warping, these rings have a cross-hatch surface design that provide excellent paraffin bonding for clamping and sectioning.
Cat. No.
Tissue-Tek
Description
Pack
Price
 
62525-W
4151
1000/
3179
Add to Cart
62525-B
4116
1000/
3179
Add to Cart
62525-G
4116
绿
1000/
3179
Add to Cart
62525-P
4116
粉红
1000/
3179
Add to Cart
62525-Y
4116
1000/
3179
Add to Cart
3. Tissue-Tek® 金属包埋模具
Made from high quality stainless steel for optimal thermal conductivity, and they have a well polished surface for easy paraffin block removal. All of the corners are rounded for good paraffin ribboning. These Base Molds are for all applications in specimen embedding with Tissue-Tek® Embedding Rings shown above.
Cat. No.
Tissue-Tek
Description MM
Pack
Price
 
62527-16
4121
模具规格16x16x6
12/
1513
Add to Cart
62527-22
4122
模具规格22x22x6
12/
1513
Add to Cart
62527-32
4123
模具规格32x25x6
12/
1513
Add to Cart
62527-38
4124
模具规格38x25x6
12/
1734
Add to Cart
62528-22
4131
模具规格22x22x12
12/
1734
Add to Cart
62528-32
4132
模具规格32x25x12
12/
1734
Add to Cart
62528-38
4133
模具规格38x25x12
12/
1734
Add to Cart
Tissue-Tek® Embedding Supplies; Sakura Finetek
1. Tissue-Tek® Mold Release, Concentrate
Use this solution to coat base molds to ensure complete separation of embedding paraffin from the mold. The working solution is prepared by adding 5 ml of Concentrate to 95 ml of ethyl alcohol or isopropyl alcohol. Molds are dipped in solution and dried prior to use.
2. Tissue-Tek® Marking Pencil
These pencils are made from soft lead, which is ideal for marking plastic cassettes.
Cat. No.
Tissue-Tek
Description
Pack
Price
 
62530-01
4141
Mold Release
4 oz
300
Add to Cart
62530-12
4141
Mold Release
12/cs
2300
Add to Cart
62531-01
4160
Marking Pencil
12/pk
300
Add to Cart
62531-06
4160
Marking Pencil
6pk/cs
1300
Add to Cart
 
Tissue-Tek® Cryomold®; Sakura Finetek一次性包埋模具
These disposable plastic Cryomolds produce a uniformly shaped flat surface specimen block with tissue freezing medium (O.C.T.) for frozen sectioning, Standard Cryomold fits directly into Cryobar® freezing wells of the Tissue-Tek® II microtome/cryostat. Use a Cryomold® adapter to adapt smaller biopsy and intermediate Cryomold® molds to freezing wells on the Cryobar® unit.
Cat. No.
Tissue-Tek
Description
Pack
Price
 
62534-25
4557
Standard, 25x20x5mm
100/pk
450
Add to Cart
62534-10
4565
Biopsy, 10x10x5mm
100/pk
450
Add to Cart
62534-15
4566
Intermediate 15x15x5mm
100/pk
450
Add to Cart
Tissue-Tek® II Microtome Object Holders; Sakura Finetek
A reusable brass thermal conductive holder with an embossed surface for the Tissue-Tek II microtome/cryostat and for conventional procedures for frozen tissue embedding.
Cat. No.
Tissue-Tek
Description
Pack
Price
 
62538-01
4560
Solid Object Holder (left image)
each
450
Add to Cart
62538-06
4560
Solid Object Holder
6/pk
1683
Add to Cart
Accessories for Tissue-Tek® CRYO3® Microtome/Cryostat Cryo 2000™
1. Cryomold® Specimen Holders
Plastic Cryomold®, round shapes with a tab for easy handling. Available in standard and biopsy configurations. The standard mold measures 22mm (D) x 5mm deep. The Biopsy mold measures 15mm (D) x 5mm deep.
Cat. No.
Tissue-Tek
Description
Pack
Price
 
62532-01
4728
CRYO3 CryoMold, Standard
100/
500
Add to Cart
62532-12
4728
CRYO3 CryoMold, Standard
1200/
3706
Add to Cart
62533-01
4730
CRYO3 Cryomold, Biopsy
100/
500
Add to Cart
62533-12
4730
CRYO3 Cryomold, Biopsy
1200/
3706
Add to Cart
2. Round Object Holders
Machined from solid aluminum with an embossed surface for holding frozen embedding blocks. Measures: 26mm top diameter (without ‘O’ ring), 28.55mm diameter with ‘O’ ring, 23mm body diameter, 16mm height .
62537-01
4726
Round Object Holder, 25mm
each
580
Add to Cart
62537-06
4726
Round Object Holder, 25mm
6/cs
3060
Add to Cart
Autopsy Baskets and Cassettes
Made from stainless steel. It is fully perforated allowing for free movement of fluids. It comes with a snap-cap which provides positive enclosure.
62310-03 is no longer available. Please see 62320-01.
Catalog #
Description
Measures, mm
Pack
Price
 
62310-12
SS Autopsy Cassettes
50(L)x25(W)x12(D)
each
1000
Add to Cart
Embedding Rings
Molded from the highest quality plastic, our Embedding Rings fit most base molds. For added convenience, there are four colors from which to choose (Blue, Pink, Green, Yellow). The large writing surface further eases identification and handling. The base measures 1 5/8″ x 1 1/8″ and the ring measures 1″ x 1″ (25 x 25 mm).
62350-W
Embedding Ring White
250/bx
800
Add to Cart
62350-B
Embedding Ring, Blue
250/bx
800
Add to Cart
62350-P
Embedding Ring, Pink
250/bx
800
Add to Cart
62350-G
Embedding Ring, Green
250/bx
800
Add to Cart
62350-Y
Embedding Ring, Yellow
250/bx
800
Add to Cart
一次性包埋盒
Our Disposable Base Mold is designed to fit all existing cassettes. Because our base molds are disposable you save time on cleaning and storing and they offer you less exposure to hazardous chemicals.
62352-07
Disposable Base Mold 7x7x5mm
500/
1400
Add to Cart
62352-15
Disposable Base Mold 15x15x5mm
500/
1400
Add to Cart
62352-24
Disposable Base Mold 24x24x5mm
500/
1400
Add to Cart
62352-30
Disposable Base Mold 30x24x5mm
500/
1400
Add to Cart
62352-37
Disposable Base Mold 37x24x5mm
500/
1400
Add to Cart
Paraffin Tissue Microarrays
Paraffin tissue microarrays (TMAs) are becoming more common in the laboratories. Current techniques including organizing, punching, and inserting tissues is time consuming and involving costly equipments.This Arraymold is the simple tool but great answer for this current technique for laboratories to perform TMAs with less time consuming, excellent results.
With this Arraymold, you can process up to 150 specimens onto one single slide with very little time consuming, minimal technical training.
The DVD instruction is accompanying with each kit, will step by step guide you to perform through the entire TMAs process. Arraymold features include:
  • Allow pathologist or researcher to view many different samples on the same single slide
  • Helpful in finding IHC markers for control or quality assurance from hundreds of samples
  • Staining markers will be more consistent when hundreds of samples are on one slide
  • Used less costly antibody
  • Arraymold kit is small enough to be stored in a drawer
  • Rubber arraymold can be reused many times without losing its flexibility.
Ordering:
Arraymold Kit A, (60 cores)
This mold generates 2 mm diameter sample core, 60 cores per mold. Kit consists of:
10 Disposable 2 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 2 mm reusable rubber Arraymold (60 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-10
Arraymold Kit A, 60 Cores
each
16983
Add to Cart
Arraymold Kit B, (150 cores)
This mold generates 1.5 mm diameter sample core, 150 cores per mold. Kit consists of
10 Disposable 2 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 1.5 mm reusable rubber Arraymold (150 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-20
Arraymold Kit B, 150 Cores
each
16983
Add to Cart
Arraymold Kit C, (150 cores)
This mold generates 4 mm diameter sample core, 15 cores per mold. Kit consists of:
10 Disposable 4 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 4 mm reusable rubber Arraymold (150 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-40
Arraymold Kit C, 15 Cores
each
16983
Add to Cart
Arraymold Kit D, (36 cores)
This mold generates 2 mm diameter sample core, 36 cores per mold. Kit consists of:
10 Disposable 2 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 2 mm reusable rubber Arraymold (36 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-15
Arraymold Kit D, 36 Cores
each
11883
Add to Cart
Arraymold Kit E, (72 cores)
This mold generates 1.5 mm diameter sample core, 72 cores per mold. Kit consists of:
10 Disposable 1.5 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 1.5 mm reusable rubber Arraymold (72 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-25
Arraymold Kit E, 72 Cores
each
11883
Add to Cart
Arraymold Kit F, (35 cores)
This mold generates 3 mm diameter sample core, 35 cores per mold. Kit consists of:
10 Disposable 3 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 4 mm reusable rubber Arraymold (35 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-45
Arraymold Kit F, 35 Cores
each
16983
Add to Cart
Arraymold Kit G, (24 cores)
This mold generates 3.5 mm diameter sample core, 24 cores per mold. Kit consists of:
10 Disposable 3.5 mm sterile dermal needle
2 Stylets (used to remove sample from needle)
1 Brass Plate (for setting cryoarray punches)
1 4 mm reusable rubber Arraymold (24 cores)
1 DVD instruction (NTSC) for showing how to use the mold.
69130-55
Arraymold Kit G, 24 Cores
each
16983
Add to Cart
Arraymold Package Kit
To save money, you can order Arraymold Package Kit. This package includes two or three Arraymold kits listed above.
  • Arraymold Kit 1: Including Arraymold Kit A and Arraymold Kit B
  • Arraymold Kit 2: Including Arraymold Kit A and Arraymold Kit C
  • Arraymold Kit 3: Including Arraymold Kit B and Arraymold Kit C
  • Arraymold Kit 4: Including all three Arraymold Kit A, B, and C
69131-01
Arraymold Package Kit 1 (A +B)
each
30566
Add to Cart
69131-02
Arraymold Package Kit 2 (A +C)
each
30566
Add to Cart
69131-03
Arraymold Package Kit 2 (B +C)
each
30566
Add to Cart
69131-04
Arraymold Package Kit 2 (A +B+C)
each
45509
Add to Cart
Spare Accessories for the ArrayMold
69130-56
Punch Needle 1.5mm
50/pk
3315
Add to Cart
69130-57
Punch Needle 2.0mm
50/pk
3315
Add to Cart
69130-58
Punch Needle 3.0mm
50/pk
3315
Add to Cart
69130-59
Punch Needle 3.5mm
50/pk
3315
Add to Cart
69130-62
Punch Needle 4.0mm
50/pk
3315
Add to Cart
69130-63
Styler 1.5mm
each
500
Add to Cart
69130-64
Styler 2.0mm
each
500
Add to Cart
69130-65
Styler 3.0mm
each
500
Add to Cart
69130-66
Styler 3.5mm
each
500
Add to Cart
69130-67
Styler 4.0mm
each
500
Add to Cart
Micro Baskets
Made from stainless steel and has a positive clip-on cover. It is ideal for processing micro specimens. It can be used as a holder for paraffin sections while removing paraffin wax. Available in medium and fine mesh. It measures: 20mm(dia.)x 5mm(deep).
62312-10
Micro Baskets, Medium Mesh
each
480
Add to Cart
62312-16
Micro Baskets, Medium Mesh
6/pk
1904
Add to Cart
62312-20
Micro Baskets, Fine Mesh
each
480
Add to Cart
62312-26
Micro Baskets, Fine Mesh
6/pk
1904
Add to Cart
Expandable Capsules
Made from stainless steel and comes with a positive snap cap. They are expandable. When closed its dimensions are 40mm(dia.)x 7mm (deep) and can be expanded to 11mm deep. Perforated top and bottom.
62314-01
Expandable Capsule
each
580
Add to Cart
62314-06
Expandable Capsule
6/pk
2720
Add to Cart
Multi-Purpose Capsules
Product 62316 no longer is available. Please see 62320.
Stainless Steel Tissue Capsules
Features:
  • A secure positive snap-on cover.
  • Perforated on the top and bottom for easy fluid circulation.
  • Can be partitioned for up to 4 sections.
The separator is not included
They measure: 37mm(I.D.) x 6mm(deep) (17/16″ x 3/16″)
62320-01
SS Tissue Capsule
each
450
Add to Cart
62320-06
SS Tissue Capsule
6/pk
1640
Add to Cart
62320-20
Tissue Capsule Separator
2/pk
450
Add to Cart
Plastic Tissue Capsules
Features:
  • Molded from polyethylene; resistant to all processing chemicals, including Zenker’s fixative.
  • A three-compartment divider which is removable for specimens up to 35mm.
  • A weighted end floats the capsule in a upright position to conserve space.
  • Perforated on the top and bottom for better circulation of the fluid.
  • Snap-on hinged cover.
They measure: 35mm(I.D.)x35mm(I.D.)x10mm(deep) (13/8×5/16)
62322-12
Plastic Tissue Capsules
12/pk
1400.00
Add to Cart
Specimen Discs
Accuray machined chrome plated aluminum discs that fit most microtomes and cryostats. They have a knurled surface for better specimen handling. Straight shank. Available in a variety of disc sizes.
62355-01
Specimen Disc, size 7/8″ (2.2cm)
each
1200
Add to Cart
62355-04
Specimen Disc, size 17/8″ (2.9cm)
each
1200
Add to Cart
62355-07
Specimen Disc, size 1½” (3.8cm)
each
1200
Add to Cart
62355-10
Specimen Disc, size 2″ (5.1cm)
each
1200
Add to Cart
Tissue Processing Capsules
These one piece plastic capsules are hinged and perfectly aligned, offering convenience and ease of use. Both the top and the bottom of the capsule have an open mesh offering maximum fluid flow during processing. A frosted marking surface assures permanent identification. These capsules are commonly used with the Embedding Ring System. They are available in two sizes and five colors.
To Use: Simply place the tissue in the capsule, close the cover to lock, and the sample is now ready for processing.
  • The 38mm(Dia.)x8mm(Deep) are intended for use with normal tissue.
  • The 29mm(Dia.)x6mm(Deep) are intended for use with Biopsy tissue
Cat. No
Color
Capsule Size
Pack
Price
 
62358-W
White
38mmx8mm
500
1785
Add to Cart
62359-W
White
29mmx6mm
500
1785
Add to Cart
Embryo Dishes
These dishes are molded from clear glass and come with a glass lid for the viewing or staining of free-floating specimens.
70543-30
Embryo Dish, 30 mm Dia x 12mm Deep
each
350
Add to Cart
70543-45
Embryo Dish, 45 mm Dia x 12mm Deep
each
450
Add to Cart
Paper Biopsy Bags
Due to the amazing demand from of our customers, EMS is re-introducing the original Paper Biopsy Bags. These bags are made from porous paper which provides maximum tissue safety during processing. The bags permit proper fluid exchange and drainage.
To Use: Simply introduce the specimen into the bag and fold its end, enclosing the cassette or capsule – you are now ready to process as usual.
Bag measurement: Overall 13⁄4” W x 3” L (45 x 75 mm), Inside Seam: 13⁄8” W (35 mm)
62326-01
Biopsy Paper Bags
100/pk
350
Add to Cart
62326-05
Biopsy Paper Bags
500/pk
800
Add to Cart
Biopsy Foam Pads
A cellular polyester blue foam that has uniform air pockets throughout the pad for optimal fluid flow. It prevents either small or fragmented tissue specimens from being lost.
To Use: Place small samples or specimen fragments between the two pads and place the specimen in a cassette or capsule.
Cat. No.
Foam Pad Size
Use With
Pack
Price
 
62325-01
25mm Diameter
29mm Capsules
1000/pk
600
Add to Cart
62325-04
35mm Diameter
38mm Capsules
1000/pk
700
Add to Cart
62325-06
25mmx32mm
1×1¼”Cassettes
1000/pk
600
Add to Cart
Biopsy Bags
  • Made from nylon cloth, 0.2mm mesh diameter. All seams are heat-sealed.
  • Allows for unrestricted fluid movement around the tissue.
  • Reduces the loss of small biopsy specimens.
  • Reduces cross contamination and pressure artifacts by using sponges.
62324-35
Nylon Biopsy Bag 35 x 50mm
100/pk
900
Add to Cart
62324-75
Nylon Biopsy Bag 45 x 74mm
100/pk
1200
Add to Cart
62324-98
Nylon Biopsy Bag 98 x 75mm
100/pk
1300
Add to Cart
 
 
 
CellSafe™ Biopsy Insert
The CellSafe is a disposable plastic biopsy insert consisting of two hinged interlocking frames with an integral nylon mesh. The mesh is extra fine and therefore ensures a high level of specimen security.
Measurements:
Overall: 28 mm x 25 mm x 5 mm high (1.1” x 1” x ¼”);
The mesh area: 18 mm x 18 mm x 2 mm high.
62327-10
CellSafe™ Biopsy Insert
100/pk.
1250
Add to Cart
Stainless Steel Biopsy Inserts
A two-part loose fitting stainless steel capsule with welded mesh that fits inside the processing cassettes preventing the loss of small or friable material while allowing interchange of fluids.
They fit all process/embedding cassettes such as 70025 and 72025 as well as Uni-Cassettes (62500).
62328-01
Stainless Steel Biopsy Insert
each
340
Add to Cart
 
Peel Away 可撕性一次性包埋盒
For plastic or paraffin embedding; no block trimming needed. Inside fingers hold ID slip which becomes embedded in the block for positive permanent identification.
70180
Truncated Mold, size 8x8mm
288/cs
2754
Add to Cart
70181
Truncated Mold, size 12x12mm
288/cs
2754
Add to Cart
70182
Square Mold, size 22x22mm
288/cs
2754
Add to Cart
70183
Rectangular Mold, size 22x30mm
288/cs
2754
Add to Cart
70184
Rectangular Mold, size 22x40mm
288/cs
2754
Add to Cart
Adjustable Embedding Mold
These two “L’s” shape and stainless steel base plate form an adjustable embedding mold, suitable for embedding various size specimens with a minimum of waste material. Available in 5 different sizes.
62361-01
Adjustable Mold, 7⁄8″ x 13⁄4″ x ½” (22x44x13mm)
each
1200
Add to Cart
62361-03
Adjustable Mold, 7⁄8″ x 13⁄4″ x 3⁄4″ (22x44x19mm)
each
1200
Add to Cart
62361-05
Adjustable Mold, 7⁄8″ x 13⁄4″ x 1″ (22x44x25mm)
each
1200
Add to Cart
62361-07
Adjustable Mold, 7⁄8″ x 13⁄4″ x 11⁄4″ (22x44x32mm)
each
1400
Add to Cart
62361-09
Adjustable Mold, 3½” x 53⁄4″ x 1″ (89x146x25mm)
each
1500
Add to Cart
Slide Coating Adhesive
Prepared from 3-aminopropyltriethoxysilane in acetone. Good for coating glass slides to adhere paraffin sections for further processing without sections falling off from the slide. READY TO USE.
71302
Slide Coating Adhesive
100 ml
650
Add to Cart
Embedding Blocks
  • Precisely machined from hard phenolic resin .
  • The faces of the embedding blocks are cut very deeply providing a firm hold of the embedded specimen.
  • Suitable for cellodin or paraffin sections.
    Available in four sizes.
62363-01
Embedding Block,
19x19x19mm High
each
900
Add to Cart
62363-02
Embedding Block,
25x25x19mm High
each
1000
Add to Cart
62363-03
Embedding Block,
38x38x19mm High
each
1100
Add to Cart
62363-04
Embedding Block,
51x51x19mm High
each
1300
Add to Cart
Embedding Molds and Mold Release Agents
For a complete selection, please see Molds and Mold Release Agent
Liquid Blocker–Super PAP Pen
The Newest Staining Guard Pen
New and improved. The Super PAP Pen outperforms the original PAP Pen. The Super PAP Pen may be used at temperatures as high as 120°F. (The old style PAP Pen worked only up to 90°F). With this high temperature feature, one can now use the Super PAP Pen to perform the peroxidase-anti-peroxidase method, ABC method, and Fluorescent antibodies method with satisfactory results.
71310
Super PAP Pen, Large
each
1000
Add to Cart
71312
Super PAP Pen, Small
each
820
Add to Cart
 
Aqua-Hold 免疫组化笔
The Aqua-Hold Pap Pen draws a liquid repellent barrier around a section of tissue. Used like a paint pen, you just draw a well on a slide. The barrier creates the proper surface tension to hold aqueous solutions such as costly antibody and other valuable specimens. After application, the barrier material is insoluble in alcohol and acetone but can be removed after staining with xylene. Each pen holds 13ml of long-lasting material, making hundreds of wells.
71311
Aqua-Hold Pen
each
800
Add to Cart
Fro-Marker
A wide tipped pen designed for use with frozen sections. The pen provides a sticky membrane which when applied to the slide and onto which the section is placed it eliminates the section from falling off, moving, or wrinkling on the slide during immunostaining procedures. The membrane is stable up to 110°C and is suitable for vigorous applications such as in-situ hybridization.
71313-10
Fro-Marker
each
1400
Add to Cart
Para-Marker
A wide tipped pen, designed for use with paraffin sections. The pen provides a sticky membrane to the glass slide which not only prevents paraffin sections from falling off but also keeps the sections flat on the slide.
71313-20
Para-Marker
each
1300
Add to Cart
Tissue Capture Pen
With one swift application of the EMS Tissue Capture Pen, you are able to change your plain glass slide into a coated one.
The EMS Tissue Capture Pen is a coating pen with a wide point (18mm) for fixing various tissue sections (paraffin embedded, frozen and formalin sections, etc.) The unique pen is unaffected by heat up to 120ºC so it offers secure and firm adhesion of the tissue section. It may be used in Microwave staining, immunostaining, in situ etc….
One application is all that is needed, and after it is applied, allow to dry for 1-2 minutes.
The EMS Tissue Capture may be used in conjunction with our Pap-Pen ( 71310) and will allow for easy placement of adherent as well as nonadherent cells on a glass slide. Immunostaining/ISH can be performed by applying a few drops of tissue suspension or liquid media onto the glass slide which is circled by the Pap-Pen, and then your section can be placed.
Ordering
71314-10
EMS Tissue Capture Pen
each
1800
Add to Cart
 

Tissue-Tek 特约代理


Tissue-Tek 特约代理

简要描述:Tissue-Tek 代理,Tissue-Tek 上海代理,Tissue-Tek 北京代理, Tissue-Tek 代理, Tissue-Tek 一级代理, Tissue-Tek 特约代理
上海金畔生物科技有限公司 Tissue-Tek 专业代理,具体产品信息欢迎电询:021-50837765

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世界*实验材料供应商 Tissue-Tek 上海金畔生物为其中国代理, Tissue-Tek 在一直是行业的*,一直为广大科研客户提供zui为优质的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Tissue-Tek 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

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包埋耗材, Capsules, 模具和记号笔

 

Tissue Tek®; Sakura Finetek包埋模具

1. Tissue-Tek® Process/Embedding Cassette

Made from chemical resistant plastic. These cassettes have an angled, texture writing surface, ensuring positive identification throughout processing, embedding, sectioning and storage. Stainless steel cover is available separay

 

Cat. No.

Tissue-Tek

标签

Pack

Price

 

62520-C

4191

透明

1500/

3519

Add to Cart

62520-W

4187

白色

1500/

3502

Add to Cart

62520-A

4129

浅绿

1500/

3876

Add to Cart

62520-B

4184

蓝色

1500/

3876

Add to Cart

62520-G

4128

金色

1500/

3910

Add to Cart

62520-GR

4183

绿色

1500/

3910

Add to Cart

62520-O

4126

橙色

1500/

3910

Add to Cart

62520-P

4182

粉红

1500/

3910

Add to Cart

62520-Y

4179

黄色

1500/

3910

Add to Cart

62520-25

4194

SS Cassette Cover

25/

731

Add to Cart

 

2. Tissue-Tek® Embedding Rings

Made from polystyrene and reinforced with the dual bar center support to prevent warping, these rings have a cross-hatch surface design that provide excellent paraffin bonding for clamping and sectioning.

 

Cat. No.

Tissue-Tek

Description

Pack

Price

 

62525-W

4151

1000/

3179

Add to Cart

62525-B

4116

1000/

3179

Add to Cart

62525-G

4116

绿

1000/

3179

Add to Cart

62525-P

4116

粉红

1000/

3179

Add to Cart

62525-Y

4116

1000/

3179

Add to Cart

 

3. Tissue-Tek® 金属包埋模具

Made from high quality stainless steel for optimal thermal conductivity, and they have a well polished surface for easy paraffin block removal. All of the corners are rounded for good paraffin ribboning. These Base Molds are for all applications in specimen embedding with Tissue-Tek® Embedding Rings shown above.

 

Cat. No.

Tissue-Tek

Description MM

Pack

Price

 

62527-16

4121

模具规格16x16x6

12/

1513

Add to Cart

62527-22

4122

模具规格22x22x6

12/

1513

Add to Cart

62527-32

4123

模具规格32x25x6

12/

1513

Add to Cart

62527-38

4124

模具规格38x25x6

12/

1734

Add to Cart

62528-22

4131

模具规格22x22x12

12/

1734

Add to Cart

62528-32

4132

模具规格32x25x12

12/

1734

Add to Cart

62528-38

4133

模具规格38x25x12

12/

1734

Add to Cart

 

Tissue-Tek® Embedding Supplies; Sakura Finetek

1. Tissue-Tek® Mold Release, Concentrate

Use this solution to coat base molds to ensure complete separation of embedding paraffin from the mold. The working solution is prepared by adding 5 ml of Concentrate to 95 ml of ethyl alcohol or isopropyl alcohol. Molds are dipped in solution and dried prior to use.

2. Tissue-Tek® Marking Pencil

These pencils are made from soft lead, which is ideal for marking plastic cassettes.

 

Cat. No.

Tissue-Tek

Description

Pack

Price

 

62530-01

4141

Mold Release

4 oz

300

Add to Cart

62530-12

4141

Mold Release

12/cs

2300

Add to Cart

62531-01

4160

Marking Pencil

12/pk

300

Add to Cart

62531-06

4160

Marking Pencil

6pk/cs

1300

Add to Cart

 

 

Tissue-Tek® Cryomold®; Sakura Finetek一次性包埋模具

These disposable plastic Cryomolds produce a uniformly shaped flat surface specimen block with tissue freezing medium (O.C.T.) for frozen sectioning, Standard Cryomold fits directly into Cryobar® freezing wells of the Tissue-Tek® II microtome/cryostat. Use a Cryomold® adapter to adapt smaller biopsy and intermediate Cryomold® molds to freezing wells on the Cryobar® unit.

 

Cat. No.

Tissue-Tek

Description

Pack

Price

 

62534-25

4557

Standard, 25x20x5mm

100/pk

450

Add to Cart

62534-10

4565

Biopsy, 10x10x5mm

100/pk

450

Add to Cart

62534-15

4566

Intermediate 15x15x5mm

100/pk

450

Add to Cart

 

Tissue-Tek® II Microtome Object Holders; Sakura Finetek

A reusable brass thermal conductive holder with an embossed surface for the Tissue-Tek II microtome/cryostat and for conventional procedures for frozen tissue embedding.

 

Cat. No.

Tissue-Tek

Description

Pack

Price

 

62538-01

4560

Solid Object Holder (left image)

each

450

Add to Cart

62538-06

4560

Solid Object Holder

6/pk

1683

Add to Cart

 

Accessories for Tissue-Tek® CRYO3® Microtome/Cryostat Cryo 2000™

1. Cryomold® Specimen Holders

Plastic Cryomold®, round shapes with a tab for easy handling. Available in standard and biopsy configurations. The standard mold measures 22mm (D) x 5mm deep. The Biopsy mold measures 15mm (D) x 5mm deep.

 

Cat. No.

Tissue-Tek

Description

Pack

Price

 

62532-01

4728

CRYO3 CryoMold, Standard

100/

500

Add to Cart

62532-12

4728

CRYO3 CryoMold, Standard

1200/

3706

Add to Cart

62533-01

4730

CRYO3 Cryomold, Biopsy

100/

500

Add to Cart

62533-12

4730

CRYO3 Cryomold, Biopsy

1200/

3706

Add to Cart

 

2. Round Object Holders

Machined from solid aluminum with an embossed surface for holding frozen embedding blocks. Measures: 26mm top diameter (without ‘O’ ring), 28.55mm diameter with ‘O’ ring, 23mm body diameter, 16mm height .

 

62537-01

4726

Round Object Holder, 25mm

each

580

Add to Cart

62537-06

4726

Round Object Holder, 25mm

6/cs

3060

Add to Cart

 

Autopsy Baskets and Cassettes

Made from stainless steel. It is fully perforated allowing for free movement of fluids. It comes with a snap-cap which provides positive enclosure.

62310-03 is no longer available. Please see 62320-01.

 

Catalog #

Description

Measures, mm

Pack

Price

 

62310-12

SS Autopsy Cassettes

50(L)x25(W)x12(D)

each

1000

Add to Cart

 

Embedding Rings

Molded from the highest quality plastic, our Embedding Rings fit most base molds. For added convenience, there are four colors from which to choose (Blue, Pink, Green, Yellow). The large writing surface further eases identification and handling. The base measures 1 5/8″ x 1 1/8″ and the ring measures 1″ x 1″ (25 x 25 mm).

 

62350-W

Embedding Ring White

250/bx

800

Add to Cart

62350-B

Embedding Ring, Blue

250/bx

800

Add to Cart

62350-P

Embedding Ring, Pink

250/bx

800

Add to Cart

62350-G

Embedding Ring, Green

250/bx

800

Add to Cart

62350-Y

Embedding Ring, Yellow

250/bx

800

Add to Cart

 

一次性包埋盒

Our Disposable Base Mold is designed to fit all existing cassettes. Because our base molds are disposable you save time on cleaning and storing and they offer you less exposure to hazardous chemicals.

 

62352-07

Disposable Base Mold 7x7x5mm

500/

1400

Add to Cart

62352-15

Disposable Base Mold 15x15x5mm

500/

1400

Add to Cart

62352-24

Disposable Base Mold 24x24x5mm

500/

1400

Add to Cart

62352-30

Disposable Base Mold 30x24x5mm

500/

1400

Add to Cart

62352-37

Disposable Base Mold 37x24x5mm

500/

1400

Add to Cart

 

Paraffin Tissue Microarrays

Paraffin tissue microarrays (TMAs) are becoming more common in the laboratories. Current techniques including organizing, punching, and inserting tissues is time consuming and involving costly equipments.This Arraymold is the simple tool but great answer for this current technique for laboratories to perform TMAs with less time consuming, excellent results.

With this Arraymold, you can process up to 150 specimens onto one single slide with very little time consuming, minimal technical training.

The DVD instruction is accompanying with each kit, will step by step guide you to perform through the entire TMAs process. Arraymold features include:

  • Allow pathologist or researcher to view many different samples on the same single slide
  • Helpful in finding IHC markers for control or quality assurance from hundreds of samples
  • Staining markers will be more consistent when hundreds of samples are on one slide
  • Used less costly antibody
  • Arraymold kit is small enough to be stored in a drawer
  • Rubber arraymold can be reused many times without losing its flexibility.

Ordering:

Arraymold Kit A, (60 cores)

This mold generates 2 mm diameter sample core, 60 cores per mold. Kit consists of:

10 Disposable 2 mm sterile dermal needle

2 Stylets (used to remove sample from needle)

1 Brass Plate (for setting cryoarray punches)

1 2 mm reusable rubber Arraymold (60 cores)

1 DVD instruction (NTSC) for showing how to use the mold.

 

69130-10

Arraymold Kit A, 60 Cores

each

16983

Add to Cart

 

Arraymold Kit B, (150 cores)

This mold generates 1.5 mm diameter sample core, 150 cores per mold. Kit consists of

10 Disposable 2 mm sterile dermal needle

2 Stylets (used to remove sample from needle)

1 Brass Plate (for setting cryoarray punches)

1 1.5 mm reusable rubber Arraymold (150 cores)

1 DVD instruction (NTSC) for showing how to use the mold.

 

69130-20

Arraymold Kit B, 150 Cores

each

16983

Add to Cart

 

Arraymold Kit C, (150 cores)

This mold generates 4 mm diameter sample core, 15 cores per mold. Kit consists of:

10 Disposable 4 mm sterile dermal needle

2 Stylets (used to remove sample from needle)

1 Brass Plate (for setting cryoarray punches)

1 4 mm reusable rubber Arraymold (150 cores)

1 DVD instruction (NTSC) for showing how to use the mold.

 

69130-40

Arraymold Kit C, 15 Cores

each

16983

Add to Cart

 

Arraymold Kit D, (36 cores)

This mold generates 2 mm diameter sample core, 36 cores per mold. Kit consists of:

10 Disposable 2 mm sterile dermal needle

2 Stylets (used to remove sample from needle)

1 Brass Plate (for setting cryoarray punches)

1 2 mm reusable rubber Arraymold (36 cores)

1 DVD instruction (NTSC) for showing how to use the mold.

 

69130-15

Arraymold Kit D, 36 Cores

each

11883

Add to Cart

 

Arraymold Kit E, (72 cores)

This mold generates 1.5 mm diameter sample core, 72 cores per mold. Kit consists of:

10 Disposable 1.5 mm sterile dermal needle

2 Stylets (used to remove sample from needle)

1 Brass Plate (for setting cryoarray punches)

1 1.5 mm reusable rubber Arraymold (72 cores)

1 DVD instruction (NTSC) for showing how to use the mold.

 

69130-25

Arraymold Kit E, 72 Cores

each

11883

Add to Cart

 

Arraymold Kit F, (35 cores)

This mold generates 3 mm diameter sample core, 35 cores per mold. Kit consists of:

10 Disposable 3 mm sterile dermal needle

2 Stylets (used to remove sample from needle)

1 Brass Plate (for setting cryoarray punches)

1 4 mm reusable rubber Arraymold (35 cores)

1 DVD instruction (NTSC) for showing how to use the mold.

 

69130-45

Arraymold Kit F, 35 Cores

each

16983

Add to Cart

 

Arraymold Kit G, (24 cores)

This mold generates 3.5 mm diameter sample core, 24 cores per mold. Kit consists of:

10 Disposable 3.5 mm sterile dermal needle

2 Stylets (used to remove sample from needle)

1 Brass Plate (for setting cryoarray punches)

1 4 mm reusable rubber Arraymold (24 cores)

1 DVD instruction (NTSC) for showing how to use the mold.

 

69130-55

Arraymold Kit G, 24 Cores

each

16983

Add to Cart

 

Arraymold Package Kit

To save money, you can order Arraymold Package Kit. This package includes two or three Arraymold kits listed above.

  • Arraymold Kit 1: Including Arraymold Kit A and Arraymold Kit B
  • Arraymold Kit 2: Including Arraymold Kit A and Arraymold Kit C
  • Arraymold Kit 3: Including Arraymold Kit B and Arraymold Kit C
  • Arraymold Kit 4: Including all three Arraymold Kit A, B, and C

 

69131-01

Arraymold Package Kit 1 (A +B)

each

30566

Add to Cart

69131-02

Arraymold Package Kit 2 (A +C)

each

30566

Add to Cart

69131-03

Arraymold Package Kit 2 (B +C)

each

30566

Add to Cart

69131-04

Arraymold Package Kit 2 (A +B+C)

each

45509

Add to Cart

 

Spare Accessories for the ArrayMold

 

69130-56

Punch Needle 1.5mm

50/pk

3315

Add to Cart

69130-57

Punch Needle 2.0mm

50/pk

3315

Add to Cart

69130-58

Punch Needle 3.0mm

50/pk

3315

Add to Cart

69130-59

Punch Needle 3.5mm

50/pk

3315

Add to Cart

69130-62

Punch Needle 4.0mm

50/pk

3315

Add to Cart

69130-63

Styler 1.5mm

each

500

Add to Cart

69130-64

Styler 2.0mm

each

500

Add to Cart

69130-65

Styler 3.0mm

each

500

Add to Cart

69130-66

Styler 3.5mm

each

500

Add to Cart

69130-67

Styler 4.0mm

each

500

Add to Cart

 

Micro Baskets

Made from stainless steel and has a positive clip-on cover. It is ideal for processing micro specimens. It can be used as a holder for paraffin sections while removing paraffin wax. Available in medium and fine mesh. It measures: 20mm(dia.)x 5mm(deep).

 

62312-10

Micro Baskets, Medium Mesh

each

480

Add to Cart

62312-16

Micro Baskets, Medium Mesh

6/pk

1904

Add to Cart

62312-20

Micro Baskets, Fine Mesh

each

480

Add to Cart

62312-26

Micro Baskets, Fine Mesh

6/pk

1904

Add to Cart

 

Expandable Capsules

Made from stainless steel and comes with a positive snap cap. They are expandable. When closed its dimensions are 40mm(dia.)x 7mm (deep) and can be expanded to 11mm deep. Perforated top and bottom.

 

62314-01

Expandable Capsule

each

580

Add to Cart

62314-06

Expandable Capsule

6/pk

2720

Add to Cart

 

Multi-Purpose Capsules

Product 62316 no longer is available. Please see 62320.

Stainless Steel Tissue Capsules

Features:

  • A secure positive snap-on cover.
  • Perforated on the top and bottom for easy fluid circulation.
  • Can be partitioned for up to 4 sections.

The separator is not included
They measure: 37mm(I.D.) x 6mm(deep) (17/16″ x 3/16″)

 

62320-01

SS Tissue Capsule

each

450

Add to Cart

62320-06

SS Tissue Capsule

6/pk

1640

Add to Cart

62320-20

Tissue Capsule Separator

2/pk

450

Add to Cart

 

Plastic Tissue Capsules

Features:

  • Molded from polyethylene; resistant to all processing chemicals, including Zenker's fixative.
  • A three-compartment divider which is removable for specimens up to 35mm.
  • A weighted end floats the capsule in a upright position to conserve space.
  • Perforated on the top and bottom for better circulation of the fluid.
  • Snap-on hinged cover.

They measure: 35mm(I.D.)x35mm(I.D.)x10mm(deep) (13/8×5/16)

 

62322-12

Plastic Tissue Capsules

12/pk

1400.00

Add to Cart

 

Specimen Discs

Accuray machined chrome plated aluminum discs that fit most microtomes and cryostats. They have a knurled surface for better specimen handling. Straight shank. Available in a variety of disc sizes.

 

62355-01

Specimen Disc, size 7/8″ (2.2cm)

each

1200

Add to Cart

62355-04

Specimen Disc, size 17/8″ (2.9cm)

each

1200

Add to Cart

62355-07

Specimen Disc, size 1½” (3.8cm)

each

1200

Add to Cart

62355-10

Specimen Disc, size 2″ (5.1cm)

each

1200

Add to Cart

 

Tissue Processing Capsules

These one piece plastic capsules are hinged and perfectly aligned, offering convenience and ease of use. Both the top and the bottom of the capsule have an open mesh offering maximum fluid flow during processing. A frosted marking surface assures permanent identification. These capsules are commonly used with the Embedding Ring System. They are available in two sizes and five colors.

To Use: Simply place the tissue in the capsule, close the cover to lock, and the sample is now ready for processing.

  • The 38mm(Dia.)x8mm(Deep) are intended for use with normal tissue.
  • The 29mm(Dia.)x6mm(Deep) are intended for use with Biopsy tissue

 

Cat. No

Color

Capsule Size

Pack

Price

 

62358-W

White

38mmx8mm

500

1785

Add to Cart

62359-W

White

29mmx6mm

500

1785

Add to Cart

 

Embryo Dishes

These dishes are molded from clear glass and come with a glass lid for the viewing or staining of free-floating specimens.

 

70543-30

Embryo Dish, 30 mm Dia x 12mm Deep

each

350

Add to Cart

70543-45

Embryo Dish, 45 mm Dia x 12mm Deep

each

450

Add to Cart

 

Paper Biopsy Bags

Due to the amazing demand from of our customers, EMS is re-introducing the original Paper Biopsy Bags. These bags are made from porous paper which provides maximum tissue safety during processing. The bags permit proper fluid exchange and drainage.

To Use: Simply introduce the specimen into the bag and fold its end, enclosing the cassette or capsule – you are now ready to process as usual.

Bag measurement: Overall 13�4� W x 3� L (45 x 75 mm), Inside Seam: 13�8� W (35 mm)

 

62326-01

Biopsy Paper Bags

100/pk

350

Add to Cart

62326-05

Biopsy Paper Bags

500/pk

800

Add to Cart

 

Biopsy Foam Pads

A cellular polyester blue foam that has uniform air pockets throughout the pad for optimal fluid flow. It prevents either small or fragmented tissue specimens from being lost.

To Use: Place small samples or specimen fragments between the two pads and place the specimen in a cassette or capsule.

 

Cat. No.

Foam Pad Size

Use With

Pack

Price

 

62325-01

25mm Diameter

29mm Capsules

1000/pk

600

Add to Cart

62325-04

35mm Diameter

38mm Capsules

1000/pk

700

Add to Cart

62325-06

25mmx32mm

1×1¼”Cassettes

1000/pk

600

Add to Cart

 

Biopsy Bags

  • Made from nylon cloth, 0.2mm mesh diameter. All seams are heat-sealed.
  • Allows for unrestricted fluid movement around the tissue.
  • Reduces the loss of small biopsy specimens.
  • Reduces cross contamination and pressure artifacts by using sponges.

 

62324-35

Nylon Biopsy Bag 35 x 50mm

100/pk

900

Add to Cart

62324-75

Nylon Biopsy Bag 45 x 74mm

100/pk

1200

Add to Cart

62324-98

Nylon Biopsy Bag 98 x 75mm

100/pk

1300

Add to Cart

 

 

 

 

 

 

CellSafe™ Biopsy Insert

The CellSafe is a disposable plastic biopsy insert consisting of two hinged interlocking frames with an integral nylon mesh. The mesh is extra fine and therefore ensures a high level of specimen security.

Measurements:
Overall: 28 mm x 25 mm x 5 mm high (1.1” x 1” x ¼”); 
The mesh area: 18 mm x 18 mm x 2 mm high.

 

62327-10

CellSafe™ Biopsy Insert

100/pk.

1250

Add to Cart

 

Stainless Steel Biopsy Inserts

A two-part loose fitting stainless steel capsule with welded mesh that fits inside the processing cassettes preventing the loss of small or friable material while allowing interchange of fluids.

They fit all process/embedding cassettes such as 70025 and 72025 as well as Uni-Cassettes (62500).

 

62328-01

Stainless Steel Biopsy Insert

each

340

Add to Cart

 

 

Peel Away 可撕性一次性包埋盒

For plastic or paraffin embedding; no block trimming needed. Inside fingers hold ID slip which becomes embedded in the block for positive permanent identification.

 

70180

Truncated Mold, size 8x8mm

288/cs

2754

Add to Cart

70181

Truncated Mold, size 12x12mm

288/cs

2754

Add to Cart

70182

Square Mold, size 22x22mm

288/cs

2754

Add to Cart

70183

Rectangular Mold, size 22x30mm

288/cs

2754

Add to Cart

70184

Rectangular Mold, size 22x40mm

288/cs

2754

Add to Cart

 

Adjustable Embedding Mold

These two “L’s” shape and stainless steel base plate form an adjustable embedding mold, suitable for embedding various size specimens with a minimum of waste material. Available in 5 different sizes.

 

62361-01

Adjustable Mold, 7⁄8″ x 13⁄4″ x ½” (22x44x13mm)

each

1200

Add to Cart

62361-03

Adjustable Mold, 7⁄8″ x 13⁄4″ x 3⁄4″ (22x44x19mm)

each

1200

Add to Cart

62361-05

Adjustable Mold, 7⁄8″ x 13⁄4″ x 1″ (22x44x25mm)

each

1200

Add to Cart

62361-07

Adjustable Mold, 7⁄8″ x 13⁄4″ x 11⁄4″ (22x44x32mm)

each

1400

Add to Cart

62361-09

Adjustable Mold, 3½” x 53⁄4″ x 1″ (89x146x25mm)

each

1500

Add to Cart

 

Slide Coating Adhesive

Prepared from 3-aminopropyltriethoxysilane in acetone. Good for coating glass slides to adhere paraffin sections for further processing without sections falling off from the slide. READY TO USE.

 

71302

Slide Coating Adhesive

100 ml

650

Add to Cart

 

Embedding Blocks

  • Precisely machined from hard phenolic resin .
  • The faces of the embedding blocks are cut very deeply providing a firm hold of the embedded specimen.
  • Suitable for cellodin or paraffin sections. 
    Available in four sizes.

 

62363-01

Embedding Block,

19x19x19mm High

each

900

Add to Cart

62363-02

Embedding Block,

25x25x19mm High

each

1000

Add to Cart

62363-03

Embedding Block,

38x38x19mm High

each

1100

Add to Cart

62363-04

Embedding Block,

51x51x19mm High

each

1300

Add to Cart

 

Embedding Molds and Mold Release Agents

For a complete selection, please see Molds and Mold Release Agent

Liquid Blocker–Super PAP Pen

The Newest Staining Guard Pen

New and improved. The Super PAP Pen outperforms the original PAP Pen. The Super PAP Pen may be used at temperatures as high as 120°F. (The old style PAP Pen worked only up to 90°F). With this high temperature feature, one can now use the Super PAP Pen to perform the peroxidase-anti-peroxidase method, ABC method, and Fluorescent antibodies method with satisfactory results.

 

71310

Super PAP Pen, Large

each

1000

Add to Cart

71312

Super PAP Pen, Small

each

820

Add to Cart

 

 

Aqua-Hold 免疫组化笔

The Aqua-Hold Pap Pen draws a liquid repellent barrier around a section of tissue. Used like a paint pen, you just draw a well on a slide. The barrier creates the proper surface tension to hold aqueous solutions such as costly antibody and other valuable specimens. After application, the barrier material is insoluble in alcohol and acetone but can be removed after staining with xylene. Each pen holds 13ml of long-lasting material, making hundreds of wells.

 

71311

Aqua-Hold Pen

each

800

Add to Cart

 

Fro-Marker

A wide tipped pen designed for use with frozen sections. The pen provides a sticky membrane which when applied to the slide and onto which the section is placed it eliminates the section from falling off, moving, or wrinkling on the slide during immunostaining procedures. The membrane is stable up to 110°C and is suitable for vigorous applications such as in-situ hybridization.

 

71313-10

Fro-Marker

each

1400

Add to Cart

 

Para-Marker

A wide tipped pen, designed for use with paraffin sections. The pen provides a sticky membrane to the glass slide which not only prevents paraffin sections from falling off but also keeps the sections flat on the slide.

 

71313-20

Para-Marker

each

1300

Add to Cart

 

 

上海金畔生物科技有限公司

TBS组织冰冻包埋剂Tissue Freezing Medium


TBS组织冰冻包埋剂Tissue Freezing Medium

简要描述:Triangle Biomedical Sciences公司生产,又称TBS包埋剂。这是冰冻切片常用到的一种包埋介质,它是一种水溶性乙二醇和树脂的混合物。该包埋试剂降低组织含水量,*的减低冰冻断裂;切片不卷曲,便于拾取切片。

详细介绍

产品咨询

Triangle Biomedical Sciences公司生产,又称TBS包埋剂。这是冰冻切片常用到的一种包埋介质,它是一种水溶性乙二醇和树脂的混合物。该包埋试剂降低组织含水量,zui大程度的减低冰冻断裂;切片不卷曲,便于拾取切片。
货号 产品名称 规格 价格
72592 Tissue Freezing Medium, clear,TFM-5 4 oz 600
72592-Y Tissue Freezing Medium, Yellow 4 oz 650
72592-G Tissue Freezing Medium, Green 4 oz 650
72592-R Tissue Freezing Medium, Red 4 oz 650
72592-B Tissue Freezing Medium, Blue 4 oz 650

Tissue-Tek® O.C.T. Compound

Sakura Finetek – This is a well known water soluble glycol’s and resins compound that provides an excellent specimen matrix for cryostat sectioning at temperatures of -10°C and below. It leaves no residue during the staining procedure.

Cat. No. Tissue-Tek Description Pack Price  
62550-01 4583 O.C.T. Compound 4 oz 230 Add to Cart
62550-12 4583 O.C.T. Compound 12/cs 1900 Add to Cart

Tissue from mice with SLE 说明书

世界*实验材料供应商 Hookelabs上海金畔生物为其中国代理, Hookelabs在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Hookelabs就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Hookelabs中国代理, Hookelabs上海代理, Hookelabs北京代理,Hookelabs广东代理, Hookelabs江苏代理Hookelabs湖北代理,Hookelabs天津,Hookelabs黑龙江代理,Hookelabs内蒙古代理,Hookelabs吉林代理,Hookelabs福建代理, Hookelabs江苏代理, Hookelabs浙江代理, Hookelabs四川代理,

 

Hookelabs是的自身免疫性疾病实验动物模型及其相关产品研发和生产企业,其创始人Suzana Marusic博士曾在惠氏制药长期担任炎性靶点验证实验室的主管,负责哮喘和自身免疫性疾病的研究,研发制备出高度相似性的哮喘和实验性自身免疫性脑脊髓炎小鼠模型,并以此验证了多个药物靶点。Suzana Marusic凭借其多年来在T细胞分离培养、细胞免疫、外周耐受机制及免疫相关疾病和肿瘤研究的丰富经验,创立了虎克实验动物模型公司,致力于为科研和药物研发提供的自身免疫性实验动物模型建立试剂盒,以及相关的实验动物组织和血清。公司目前的实验动物模型主要包括胶原诱导的关节炎模型、实验性自身免疫性脑脊髓炎模型、系统红斑狼疮、炎性肠病等模型的制备试剂盒,以及多种免疫状态下动物组织和血清样本。虎克实验动物模型提供的模型制备试剂盒以预充免疫原的注射器为主,操作简单,结果可靠,可重复性高。 

 

 

 

 

 

Tissue from mice with SLE

SLE组小鼠组织

用于分析基因表达,蛋白质生产和生物标志物

自发发生与系统性红斑狼疮(SLE)相似的疾病的小鼠品系的冷冻组织。

  • 分析基因表达,蛋白质存在和生物标志物在组织中没有生物

随着疾病的发展,节省老化老鼠; 组织可从库存中获得,没有提前期。

  • 根据年龄或蛋白尿历史从我们的库存中选择单个小鼠

具有蛋白尿评分和每个样品体重的完整病史。

  • 来自环磷酰胺治疗小鼠的组织可用于比较(阳性对照治疗)

来自(NZB x NZW)F1小鼠的组织

26至52周龄的女性(NZB x NZW)F1杂交(NZBWF1)小鼠的冷冻组织(年龄由订购者选择)。

这些杂交小鼠在人类患者中自发发展出类似于系统性红斑狼疮(SLE)的多基因自身免疫性疾病。早在20周龄时,可以检测到抗核和抗dsDNA抗体。在36〜45周观察到蛋白尿和肾脏病理(免疫复合性肾小球肾炎)。

每周从20周起记录体重和蛋白尿评分。治疗通常开始于23至26周。

Cat # Mouse strain Treatment/proteinuria score
LZ-0100 (NZB x NZW)F1 hybrid (NZBWF1) Cyclophosphamide-treated (no or mild proteinuria)
LZ-0101 (NZB x NZW)F1 hybrid (NZBWF1) Vehicle-treated or naive (severe proteinuria)

来自MRL / lpr小鼠的组织

12〜20周龄女性MRL / lpr小鼠的冷冻组织(订购时由顾客选择年龄)。

MRL / lpr小鼠在Fas基因中自发突变是纯合的,并发展淋巴结肿大(淋巴结肿大),脾肿大(脾脏增大)和全身自身免疫。

自身免疫反应在这些小鼠中自发发生,导致人类中类似SLE的关节炎和肾小球肾炎。早期8周龄时可以检测到抗核和抗dsDNA抗体。发现蛋白尿和肾脏病理(免疫复合性肾小球肾炎)在16至28周龄。

每周从11周起记录体重和蛋白尿评分。治疗通常在12周开始。

Cat # Mouse strain Treatment/proteinuria score
LZ-0200 MRL/lpr Cyclophosphamide-treated (no or mild proteinuria)
LZ-0201 MRL/lpr Vehicle-treated or naive (severe proteinuria)

样品选择

每个产品单元包含来自单个小鼠的肾脏和脾脏组织,全血和血清。

组织准备

立即收集血液用于血清,收集器官并在液氮中快速冷冻。将血清分离并冷冻至-80℃。

所有组织储存在-80℃直到出货。

详细内容

数量 描述
1 肾脏,在标准的1.5 mL低温管中冷冻。
1 半脾,冷冻在标准的1.5 mL低温管中。
150μL 全血,用标准的1.5 mL低温管冷冻。
150μL 血清,在标准的1.5 mL低温管中冷冻。
1 电子表格(通过电子邮件),终止年龄,
蛋白尿评分历史,体重史,
以及每个样品的组织重量或体积。

管标有鼠标ID,治疗,终止年龄,组织类型和组织重量或体积。

每个产品单元包含来自单个小鼠的组织和血清。

存储

保持冷冻直至使用。建议在-80℃下储存。

 

Tissue Solutions品牌代理

Tissue Solutions

简要描述:

Tissue Solutions是人体优质组织样本的提供者。

Tissue Solutions是人体优质组织样本的提供者。该公司拥有高效解决方案的全球网络,您可以信赖、高效地采购符合最高标准的生物材料。Tissue Solutions是一家通过ISO 9001:2015认证的公司。


Through Tissue Solutions’ global network you can reliably and efficiently source valuable biological material adhering to the highest possible ethical standards. We are an ISO 9001:2015 certified company and offer a single access point for a wide range of diseased and normal tissues in fresh, frozen, and FFPE formats.


产品列表:


序号

品牌

名称

货号

规格

1

Tissue Solutions

BM Cryo Plasma

D326778

2 mL

2

Tissue Solutions

BM Cryo Plasma – AML

E895

2 mL

3

Tissue Solutions

BM MNC

132975

Custom

4

Tissue Solutions

BM Cryo MNC

140936

25 M Cells

5

Tissue Solutions

BM Cryo MNC

132942

100 M Cells

6

Tissue Solutions

BM MSC P1

E164

Custom

7

Tissue Solutions

BM Cryo MSC P1

1 M Cells

8

Tissue Solutions

BM Cryo CD133

100K Cells

9

Tissue Solutions

BM Cryo CD34

D327458

100 K Cells

10

Tissue Solutions

BM Cryo CD34

D326437

250 K Cells

11

Tissue Solutions

BM Cryo CD34

E109

500 K Cells

12

Tissue Solutions

BM Cryo CD34

E206

2 M Cells

13

Tissue Solutions

CB Cryo Plasma

E604

Custom

14

Tissue Solutions

CD34 Depleted CB MNCs

E737

Custom

15

Tissue Solutions

CB Cryo MNC

25 M Cells

MatTek品牌代理

MatTek

简要描述:

Mattek公司处于组织工程的前沿,是世界的创新型三维重建人体组织模型的生产企业。

Mattek公司处于组织工程的前沿,是世界的创新型三维重建人体组织模型的生产企业。


MatTek Corporation is at the forefront of tissue engineering and is a world leader in the production of innovative 3D reconstructed human tissue models. Our skin, ocular, and respiratory tissue models are used in regulatory toxicology (OECD, EU guidelines) and address toxicology and efficacy concerns throughout the cosmetics, chemical, pharmaceutical and household product industries.


品牌

货号

名称

规格

MatTek

NHBE-CRY

Epthetial cells

>500cells

MatTek

NHBE-CRY-DS

Epthetial cells

>500cells

MatTek

NHBE-BM

Epthetial cells

500ml

X-Large Cell and Tissue Collection Kits


X-Large Cell and Tissue Collection Kits

简要描述:incell,incell代理,incell上海代理,incell代理,incell北京代理,incell中国代理

详细介绍

产品咨询

incell,incell代理,incell上海代理,incell代理,incell北京代理,incell中国代理

上海金畔生物科技有限公司incell代理,竭诚为您提供incell代理产品,产品*,货到付款,实验无忧!    

X-Large Cell and Tissue Collection Kits

CTK40-X适用于常规组织及细胞的收集、运输及保存, CTK40-X1适用于淋巴、血样或骨髓等组织样品的收集、运输及保存。样品体积zui大为8 L

试剂盒内含:

18L无菌收集盒或管(用于血样等液体样品)

2)无菌转移液500ml*2: CTK40-XZTM:A2™ (Cat. #CTK20-M,含克林霉素,庆大霉素及两性霉素) CTK40-X1ZTM:A1™ (Cat. #CTK20-M1,含克林霉素,庆大霉素)

3)无菌塑料组织转运袋

4)各式标签及冰袋

 

订货信息:

货号

品名

CAS

价格¥

规格

品牌

CTK40-XL

COLLECTION KITS , X-Large,

 

6460

 

INCELL Corporation

                                                  

上海金畔生物科技有限公司
杨建辉
上海市浦东绣川路5611102
021-50837765 传真 :  WWW.78BIO.COM 产品查询 CX.78BIO.COM
 

我们公司zui大优势是强大的采购,
1:基本什么都能进口,血清,抗体,耗材,还有部分限制进口的,
2 货品全,现经营过700多个品牌,基本所有生物试剂耗材都可以进口,特别是冷偏的产品那就更有优势,
3:提供加急服务,一般1-2周到货,超过时限加急费全免
4:价格公道,绝大部分价格有优势,当然不能保证100%产品都有价格优势,
5:良好的信誉,大部分客户我们提供货到付款服务,客户包括清华,北大 交大 复旦,中山等100多所大学,ROCHE,阿斯利康,国药 fisher500多家公司
6:我们还是santa,Advanced Biotechnologies Inc;Athens Research & Technology, bangs, BBInternational, crystalchem, dianova, FD Neurotechnologies, Inc. FormuMax Scientific, Inc; Genebridege; Glycotope Biotechnology GmbH; iduron; Innovative Research of America Ludger  neuroprobe; omicronbio; Polysciences; prospecbi; QA-BIOquickzymeRESEARCH DIETS, INCsterlitechsysyTriLink BioTechnologies, Incworthington-biochem;zyagen;……几十家国外公司代理
7:我们还从事invitrogenqiagen; abcam ;sigma;neb; roche;merck; rnd; BD; GE; pierce; BioLegend….等*批发 

Large Cell and Tissue Collection Kits


Large Cell and Tissue Collection Kits

简要描述:incell,incell代理,incell上海代理,incell代理,incell北京代理,incell中国代理

详细介绍

产品咨询

incell,incell代理,incell上海代理,incell代理,incell北京代理,incell中国代理

上海金畔生物科技有限公司incell代理,竭诚为您提供incell代理产品,产品*,货到付款,实验无忧!    

Large Cell and Tissue Collection Kits

CTK30-L适用于常规组织及细胞的收集、运输及保存,CTK30-L1适用于淋巴、血样或骨髓等组织样品的收集、运输及保存。样品体积zui大为4.5 L

试剂盒内含:

14.5L无菌收集盒或管(用于血样等液体样品)

2)无菌转移液500ml*1: Cat. #CTK30-LZTM:A2™ (Cat. #CTK20-M,含克林霉素,庆大霉素及两性霉素) Cat. #CTK30-L1ZTM:A1™ (Cat. #CTK20-M1,含克林霉素,庆大霉素)

3)无菌塑料组织转运袋

4)各式标签及冰袋

订货信息:

货号

品名

CAS

价格¥

规格

品牌

CTK30-L

COLLECTION KITS , Large,

 

5780

 

INCELL Corporation

                                                  

上海金畔生物科技有限公司
杨建辉
上海市浦东绣川路5611102
021-50837765 传真 :  WWW.78BIO.COM 产品查询 CX.78BIO.COM
 

我们公司zui大优势是强大的采购,
1:基本什么都能进口,血清,抗体,耗材,还有部分限制进口的,
2 货品全,现经营过700多个品牌,基本所有生物试剂耗材都可以进口,特别是冷偏的产品那就更有优势,
3:提供加急服务,一般1-2周到货,超过时限加急费全免
4:价格公道,绝大部分价格有优势,当然不能保证100%产品都有价格优势,
5:良好的信誉,大部分客户我们提供货到付款服务,客户包括清华,北大 交大 复旦,中山等100多所大学,ROCHE,阿斯利康,国药 fisher500多家公司
6:我们还是santa,Advanced Biotechnologies Inc;Athens Research & Technology, bangs, BBInternational, crystalchem, dianova, FD Neurotechnologies, Inc. FormuMax Scientific, Inc; Genebridege; Glycotope Biotechnology GmbH; iduron; Innovative Research of America Ludger  neuroprobe; omicronbio; Polysciences; prospecbi; QA-BIOquickzymeRESEARCH DIETS, INCsterlitechsysyTriLink BioTechnologies, Incworthington-biochem;zyagen;……几十家国外公司代理
7:我们还从事invitrogenqiagen; abcam ;sigma;neb; roche;merck; rnd; BD; GE; pierce; BioLegend….等*批发 

RNAsafe动植物组织RNA稳定液|RNAsafe Tissue Stabilizer

RNAsafe动植物组织RNA稳定液|RNAsafe Tissue Stabilizer

产品说明书

FAQ

COA

已发表文献

产品描述

RNA酶广泛存在环境中,很容易降解新鲜采取的动植物组织、血液、体液等样本中的RNA,从而使得研究人员在收集完新鲜样本需要做液氮速冻,或者立即进行RNA的纯化,这给研究人员的户外工作带来诸多不便。

RNAsafe动植物组织稳定液(RNAsafe Tissue Stabilizer)是专门开发用于稳定并保护采集样本内RNA,作为一种无毒溶液,可迅速渗入组织,灭活内源RNase。

 

产品特点

操作极其简便:只需要将新鲜组织切成合适大小(≤0.5 cm)的组织块,浸入5~10倍体积的RNAsafe中即可。

稳定周期长:RNAsafe浸润的组织/细胞可在37℃保存1天,室温保存1周,4℃保存4周,-20℃/-80℃长期保存。

下游兼容性广:几乎兼容于所有的RNA分离方法,如HieffTM Total RNA Extraction Reagent(货号10606ES60),以及几乎所

有的商业化RNA抽提试剂盒,包括离心柱抽提法,磁珠纯化法等。

材料应用性广:可用于多种动植物组织(如脑、心脏、肝脏、胰脏、肾脏、脾脏、睾丸、肌肉、植物的茎叶等)的保存,还可用于酵母、组织培养细胞等。

 

操作流程

1. 样品处理

A. 动物组织:RNAsafe稳定液不会破坏动物组织结构的完整性。
处理过程:将组织切成合适大小后(≤0.5 cm),然后加入5倍体积RNAsafe。
注:已浸入RNAsafe的组织经平衡一段时间后,可从溶液中取出,切成更小的组织块(≤0.5 cm大小),再重新放回RNAsafe。
注意:某些组织(如骨头)具有比较弱的液体渗透性,不适合用本品进行RNA保护。

B. 植物组织:本品对于柔软的植物组织(如嫩叶,嫩茎)可直接渗透,且达到理想的RNA保护效果。
处理过程:将组织切成合适的大小(≤0.5 cm)后,加入5倍体积的RNAsafe稳定液。
注:某些植物组织天生具有的屏障(如叶子表面的蜡层)可阻碍RNAsafe渗透,对于此类组织,往往需要破坏这些屏障层以允许溶液的浸透。

C. 组培细胞、白细胞等:按照标准实验操作收集细胞沉淀(用PBS清洗1-2次)。之后加入5~10倍体积的RNAsafe。
D. 酵母:离心弃上清,收集约108个细胞(12,000g,2 min)。加入0.5~1 mLRNAsafe。
长期保存应置于RNAsafe中,4℃放置1小时。然后离心弃上清,收集酵母细胞(12,000g,5 min),-80℃保存。

2. 样品保存

A. -80℃保存:样品结冻保存。对于归档的样本,建议-80℃保存。该保存条件下,样本可长期保存,保护效果最佳。
操作过程:样本4℃浸透过夜,然后再转移至-80℃。
如果中间需要做样本的融化,建议取出4℃浸透过夜的组织或离心收集细胞,然后再冻存于-80℃。RNAsafe保护后的样本抽提RNA时,可直接放在室温融化,之后重新冻上,不会造成RNA量的严重损失或者RNA完整性的明显破坏。

B. -20℃保存:同样适用于归档样本的保存。样本不会在-20℃结冻,但可能会形成结晶。该保存条件下,样本可长期保存,不会对后续RNA分离造成影响。
操作过程:样本4℃浸透过夜,然后再转移至-20℃。RNAsafe保护后的样本抽提RNA时,可直接放在室温融化,之后重新冻上,不会造成RNA量的严重损失或者RNA完整性的明显破坏。

C. 4℃保存:大部分样品放到RNAsafe中,可稳定保护1个月,不会有明显的RNA降解发生。

D. 25℃(室温)保存:建议低温环境保存。若条件不允许,请将RNAsafe稳定液于冰上冷却几个小时,然后将样品浸入此溶液中,室温稳定保存1周,不会有明显的RNA降解发生。
E. 37℃保存:纯化的RNA样本于37℃孵育24 h,结构依然完整。

3. RNA提取
因RNAsafe灭活RNase的过程是可逆的,受保护的样本使用前务必不要清洗掉RNAsafe。

A. 组织样本:用灭菌镊子从RNAsafe中取出组织块,然后用干净的吸水纸吸掉表面液体。之后立即置于裂解液中匀浆。

B:细胞样本:由于RNAsafe密度较大,此时需要使用大于普通介质的离心力。一般来说,保存在RNAsafe中的细胞能承受更高的转速,并维持细胞的完整性。大部分细胞可通过5,000g,5 min离心收集沉淀。
注意:由于不同细胞承受离心力会有差异,建议先用少量的细胞进行离心速度承受性的测试。

C. 抽提RNA:按照RNA抽提Protocol进行即可。

 

运输与保存方法

室温运输,室温保存,有效期5年。

 

注意事项

1. 经RNAsafe处理的样本还可做基因组DNA的提取,甚至蛋白质的提取。但由于RNAsafe会变性蛋白质,因此不适合天然蛋白的提取。

2. 本RNAsafe室温保存过程中可能会产生一些沉淀,在使用前只需放到37℃温育片刻,并间断摇晃重溶,不影响使用。

3. 本RNAsafe仅适用于新鲜组织,不可用于冰冻组织。若需要保护冰冻组织内RNA的完整性,可使用我司的RNAsafe-ICE冰冻动植物组织RNA稳定-过渡溶液(货号:10605ES60),即可将冰冻组织恢复为匀浆用的组织状态,并进行RNA抽提的过程中,维持RNA的稳定性。

4. 为了您的安全和健康,请穿实验服并佩戴一次性手套操作。

5. 本产品仅作科研用途!

 

相关产品

产品名称

货号

规格

RNAsafe-ICE冰冻动植物组织RNA稳定-过渡溶液

10605ES60

100 mL

Proteinase K 蛋白酶K

10401ES60/80

100 mg/1 g

Lysozyme溶菌酶

10402ES08/50

5/50 g

Lyticase (10 U/μL) 溶壁酶

10403ES81/88/ 92

1500/3000/10000 U

Snailase 蜗牛酶

10404ES08/10

5/10 g

TRIeasyTM Total RNA Extraction Reagent(同Trizol)HOT

10606ES60

100 mL

 

HB210722

Q准备加这个溶液到冰冻组织里然后保存两三个月,加完这个以后放-80没问题吧?

A不建议用于冰冻组织,若需要保护冰冻组织内 RNA 的完整性,可使用我司的 RNAsafe-ICE冰冻动植物组织RNA稳定-过渡溶液(货号:10605ES60),即可将冰冻组织恢复为匀浆用的组织状态,并进行后续的RNA提取,也可以置于-20℃长期保存,维持RNA的稳定性。

Q:保存细胞或组织的话可以保存多久?

A:在37℃下可稳定1天, 25℃下1周, 4℃下1个月, -20℃或-80℃下可以长期保存。

Q:处理新鲜细胞或组织后,可以直接冻-20℃或-80℃吗?

A:需要在4℃充分浸润后冻存保存,可以过夜浸润。

[1] Zhang Y, Wen J, Liu D, et al. Demethylenetetrahydroberberine alleviates nonalcoholic fatty liver disease by inhibiting the NLRP3 inflammasome and oxidative stress in mice. Life Sci. 2021;281:119778. doi:10.1016/j.lfs.2021.119778(IF:5.037)

产品描述

RNA酶广泛存在环境中,很容易降解新鲜采取的动植物组织、血液、体液等样本中的RNA,从而使得研究人员在收集完新鲜样本需要做液氮速冻,或者立即进行RNA的纯化,这给研究人员的户外工作带来诸多不便。

RNAsafe动植物组织稳定液(RNAsafe Tissue Stabilizer)是专门开发用于稳定并保护采集样本内RNA,作为一种无毒溶液,可迅速渗入组织,灭活内源RNase。

 

产品特点

操作极其简便:只需要将新鲜组织切成合适大小(≤0.5 cm)的组织块,浸入5~10倍体积的RNAsafe中即可。

稳定周期长:RNAsafe浸润的组织/细胞可在37℃保存1天,室温保存1周,4℃保存4周,-20℃/-80℃长期保存。

下游兼容性广:几乎兼容于所有的RNA分离方法,如HieffTM Total RNA Extraction Reagent(货号10606ES60),以及几乎所

有的商业化RNA抽提试剂盒,包括离心柱抽提法,磁珠纯化法等。

材料应用性广:可用于多种动植物组织(如脑、心脏、肝脏、胰脏、肾脏、脾脏、睾丸、肌肉、植物的茎叶等)的保存,还可用于酵母、组织培养细胞等。

 

操作流程

1. 样品处理

A. 动物组织:RNAsafe稳定液不会破坏动物组织结构的完整性。
处理过程:将组织切成合适大小后(≤0.5 cm),然后加入5倍体积RNAsafe。
注:已浸入RNAsafe的组织经平衡一段时间后,可从溶液中取出,切成更小的组织块(≤0.5 cm大小),再重新放回RNAsafe。
注意:某些组织(如骨头)具有比较弱的液体渗透性,不适合用本品进行RNA保护。

B. 植物组织:本品对于柔软的植物组织(如嫩叶,嫩茎)可直接渗透,且达到理想的RNA保护效果。
处理过程:将组织切成合适的大小(≤0.5 cm)后,加入5倍体积的RNAsafe稳定液。
注:某些植物组织天生具有的屏障(如叶子表面的蜡层)可阻碍RNAsafe渗透,对于此类组织,往往需要破坏这些屏障层以允许溶液的浸透。

C. 组培细胞、白细胞等:按照标准实验操作收集细胞沉淀(用PBS清洗1-2次)。之后加入5~10倍体积的RNAsafe。
D. 酵母:离心弃上清,收集约108个细胞(12,000g,2 min)。加入0.5~1 mLRNAsafe。
长期保存应置于RNAsafe中,4℃放置1小时。然后离心弃上清,收集酵母细胞(12,000g,5 min),-80℃保存。

2. 样品保存

A. -80℃保存:样品结冻保存。对于归档的样本,建议-80℃保存。该保存条件下,样本可长期保存,保护效果最佳。
操作过程:样本4℃浸透过夜,然后再转移至-80℃。
如果中间需要做样本的融化,建议取出4℃浸透过夜的组织或离心收集细胞,然后再冻存于-80℃。RNAsafe保护后的样本抽提RNA时,可直接放在室温融化,之后重新冻上,不会造成RNA量的严重损失或者RNA完整性的明显破坏。

B. -20℃保存:同样适用于归档样本的保存。样本不会在-20℃结冻,但可能会形成结晶。该保存条件下,样本可长期保存,不会对后续RNA分离造成影响。
操作过程:样本4℃浸透过夜,然后再转移至-20℃。RNAsafe保护后的样本抽提RNA时,可直接放在室温融化,之后重新冻上,不会造成RNA量的严重损失或者RNA完整性的明显破坏。

C. 4℃保存:大部分样品放到RNAsafe中,可稳定保护1个月,不会有明显的RNA降解发生。

D. 25℃(室温)保存:建议低温环境保存。若条件不允许,请将RNAsafe稳定液于冰上冷却几个小时,然后将样品浸入此溶液中,室温稳定保存1周,不会有明显的RNA降解发生。
E. 37℃保存:纯化的RNA样本于37℃孵育24 h,结构依然完整。

3. RNA提取
因RNAsafe灭活RNase的过程是可逆的,受保护的样本使用前务必不要清洗掉RNAsafe。

A. 组织样本:用灭菌镊子从RNAsafe中取出组织块,然后用干净的吸水纸吸掉表面液体。之后立即置于裂解液中匀浆。

B:细胞样本:由于RNAsafe密度较大,此时需要使用大于普通介质的离心力。一般来说,保存在RNAsafe中的细胞能承受更高的转速,并维持细胞的完整性。大部分细胞可通过5,000g,5 min离心收集沉淀。
注意:由于不同细胞承受离心力会有差异,建议先用少量的细胞进行离心速度承受性的测试。

C. 抽提RNA:按照RNA抽提Protocol进行即可。

 

运输与保存方法

室温运输,室温保存,有效期5年。

 

注意事项

1. 经RNAsafe处理的样本还可做基因组DNA的提取,甚至蛋白质的提取。但由于RNAsafe会变性蛋白质,因此不适合天然蛋白的提取。

2. 本RNAsafe室温保存过程中可能会产生一些沉淀,在使用前只需放到37℃温育片刻,并间断摇晃重溶,不影响使用。

3. 本RNAsafe仅适用于新鲜组织,不可用于冰冻组织。若需要保护冰冻组织内RNA的完整性,可使用我司的RNAsafe-ICE冰冻动植物组织RNA稳定-过渡溶液(货号:10605ES60),即可将冰冻组织恢复为匀浆用的组织状态,并进行RNA抽提的过程中,维持RNA的稳定性。

4. 为了您的安全和健康,请穿实验服并佩戴一次性手套操作。

5. 本产品仅作科研用途!

 

相关产品

产品名称

货号

规格

RNAsafe-ICE冰冻动植物组织RNA稳定-过渡溶液

10605ES60

100 mL

Proteinase K 蛋白酶K

10401ES60/80

100 mg/1 g

Lysozyme溶菌酶

10402ES08/50

5/50 g

Lyticase (10 U/μL) 溶壁酶

10403ES81/88/ 92

1500/3000/10000 U

Snailase 蜗牛酶

10404ES08/10

5/10 g

TRIeasyTM Total RNA Extraction Reagent(同Trizol)HOT

10606ES60

100 mL

 

HB210722

Q准备加这个溶液到冰冻组织里然后保存两三个月,加完这个以后放-80没问题吧?

A不建议用于冰冻组织,若需要保护冰冻组织内 RNA 的完整性,可使用我司的 RNAsafe-ICE冰冻动植物组织RNA稳定-过渡溶液(货号:10605ES60),即可将冰冻组织恢复为匀浆用的组织状态,并进行后续的RNA提取,也可以置于-20℃长期保存,维持RNA的稳定性。

Q:保存细胞或组织的话可以保存多久?

A:在37℃下可稳定1天, 25℃下1周, 4℃下1个月, -20℃或-80℃下可以长期保存。

Q:处理新鲜细胞或组织后,可以直接冻-20℃或-80℃吗?

A:需要在4℃充分浸润后冻存保存,可以过夜浸润。

[1] Zhang Y, Wen J, Liu D, et al. Demethylenetetrahydroberberine alleviates nonalcoholic fatty liver disease by inhibiting the NLRP3 inflammasome and oxidative stress in mice. Life Sci. 2021;281:119778. doi:10.1016/j.lfs.2021.119778(IF:5.037)

Tissue Solutions品牌代理

Tissue Solutions品牌介绍

简要描述:

Tissue Solutions品牌介绍是人体优质组织样本的提供者。该公司拥有高效解决方案的网络,您可以信赖、高效地采购符合高标准的生物材料。Tissue Solutions是一家通过ISO 9001:2015认证的公司。

一、TissueSolutions是人体优质组织样本的提供者。该公司拥有高效解决方案的网络,您可以信赖、高效地采购符合高标准的生物材料。TissueSolutions是一家通过ISO9001:2015认证的公司。  


二、


三、Tissue Solutions品牌介绍产品列表:

 

序号

品牌

名称

货号

规格

1

Tissue Solutions

BM Cryo Plasma

D326778

2 mL

2

Tissue Solutions

BM Cryo Plasma – AML

E895

2 mL

3

Tissue Solutions

BM MNC

132975

Custom

4

Tissue Solutions

BM Cryo MNC

140936

25 M Cells

5

Tissue Solutions

BM Cryo MNC

132942

100 M Cells

6

Tissue Solutions

BM MSC P1

E164

Custom

7

Tissue Solutions

BM Cryo MSC P1

1 M Cells

8

Tissue Solutions

BM Cryo CD133

100K Cells

9

Tissue Solutions

BM Cryo CD34

D327458

100 K Cells

10

Tissue Solutions

BM Cryo CD34

D326437

250 K Cells

11

Tissue Solutions

BM Cryo CD34

E109

500 K Cells

12

Tissue Solutions

BM Cryo CD34

E206

2 M Cells

13

Tissue Solutions

CB Cryo Plasma

E604

Custom

14

Tissue Solutions

CD34 Depleted CB MNCs

E737

Custom

15

Tissue Solutions

CB Cryo MNC

25 M Cells