| 来源 |
应用 |
交叉反应性 |
分子量 |
| Rabbit |
WB, IF, ICC,IF |
– |
– |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB |
IP |
IF |
IHC |
ICC |
FC |
ELISA |
ChIP |
| 1:1000 |
– |
1:100 |
– |
1:50 |
– |
– |
– |
抗体详细信息如下:
|
About this Antibody
|
|
Name
|
HA Tag Rabbit Polyclonal Antibody
|
|
Category
|
Polyclonal antibody (pAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
Affinity purification
|
|
About the Immunogen
|
|
Immunogen
|
This antibody is produced by immunizing rabbits with a synthetic peptide YPYDVPDYA (KLH-coupled).
|
|
Gene ID
|
–
|
|
SwissProt
|
–
|
|
Synonyms
|
N/A
|
|
Category
|
Related Products
|
|
Background
|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA epitope (YPYDVPDYA) and are expressed in prokaryotic and eukaryotic cells.
|
包装清单：
| 产品编号 |
产品名称 |
包装 |
| AF0039 |
HA Tag Rabbit Polyclonal Antibody |
100μl |
| AZ100 |
Western一抗稀释液 |
100ml |
| — |
说明书 |
1份 |
保存条件：
HA Tag Rabbit Polyclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用本产品的文献：
1. Ruirui Jia, Zhenxing Song, Jiamei Lin, Zhengguo Li, Ge Shan, Chuan Huang
Gawky modulates MTF-1-mediated transcription activation and metal discrimination
Nucleic Acids Res. 2021 Jun 21;49(11):6296-6314.;doi: 10.1093/nar/gkab474. (IF 11.501)
2. Jiawei Cao, Du Wu, Guang Wu, Yaqi Wang, Tianhao Ren, Yang Wang, Yingshuai Lv, Wei Sun, Jieyi Wang, Changrui Qian, Licai He, Kaiyan Yang, Hongzhi Li, Haihua Gu
USP35, regulated by estrogen and AKT, promotes breast tumorigenesis by stabilizing and enhancing transcriptional activity of estrogen receptor α
Cell Death Dis. 2021 Jun 15;12(6):619.;doi: 10.1038/s41419-021-03904-4. (IF 6.304)
3. Ying Zhou, Jian Guo, Xinyu Wang, Yang Cheng, Jianwen Guan, Priyam Barman, Ming-An Sun, Yuanyuan Fu, Wanhong Wei, Congjing Feng, Mary A Lilly, Youheng Wei
FKBP39 controls nutrient dependent Nprl3 expression and TORC1 activity in Drosophila
Cell Death Dis. 2021 Jun 2;12(6):571.;doi: 10.1038/s41419-021-03860-z. (IF 6.304)
4. Chen H, Xue L, Huang H, Wang H, Zhang X, Zhu W, Wang Z, Wang Z, Wu H
Synergistic effect of Nutlin-3 combined with MG-132 on schwannoma cells through restoration of merlin and p53 tumour suppressors.
EBioMedicine. 2018 Oct;36:252-265. (IF 5.736)
5. Zhang L, Liu X, Liu J, Ma X, Zhou Z, Song Y, Cao B
miR-26a promoted endometrial epithelium cells (EECs) proliferation and induced stromal cells (ESCs) apoptosis via the PTEN-PI3K/AKT pathway in dairy goats.
J Cell Physiol. 2018 Jun;233(6):4688-4706. (IF 5.546)
6. Xi Zhang, Jingjing Chen, Qiao Dong, Jinying Zhu, Ruihao Peng, Chuanyu He, Yuzhuo Li, Ruiqi Lin, Pengfei Jiang, Min Zheng, Huan Zhang, Shiwei Liu, Zeliang Chen
Lysine Acylation Modification Landscape of Brucella abortus Proteome and its Virulent Proteins
Front Cell Dev Biol. 2022 Mar 1;10:839822.;doi: 10.3389/fcell.2022.839822. (IF 5.201)
7. Liang J, She X, Chen J, Zhai Y, Liu Y, Zheng K, Gong Y, Zhu H, Luo X, Sun X
Identification of novel PROM1 mutations responsible for autosomal recessive maculopathy with rod-cone dystrophy.
GRAEF ARCH CLIN EXP. 2019 Mar;257(3):619-628. (IF 2.396)
8. Chen L, Lin L, Xian N, Zheng Z
Annexin A2 regulates glioma cell proliferation through the STAT3‑cyclin D1 pathway.
Oncol Rep. 2019 Jul;42(1):399-413. (IF 3.417)
9. Yuwei Wang, Xiaomeng Li, Yang Yu, Jian Liang, Yang Liu, Yuhong Chen, Xinyue Bai, Jieqiong Chen, Fenghua Wang, Xueting Luo, Xiaodong Sun
Modeling Cone/Cone-Rod Dystrophy Pathology by AAV-Mediated Overexpression of Mutant CRX Protein in the Mouse Retina
Transl Vis Sci Technol. 2021 Jun 1;10(7):25.;doi: 10.1167/tvst.10.7.25.
10. Yafang Wang, Shu Liu, Yuanqi Zhai, Yang Liu, Xiaoling Wan, Wenqiu Wang, Fenghua Wang, Xiaodong Sun
Identification of a novel RPGR mutation associated with X-linked cone-rod dystrophy in a Chinese family
BMC Ophthalmol. 2021 Nov 20;21(1):401.;doi: 10.1186/s12886-021-02166-0. (IF 1.413)
一键复制产品信息 
一键复制产品信息 
一键复制产品信息 
一键复制产品信息 
一键复制产品信息 
一键复制产品信息 
一键复制产品信息 
一键复制产品信息 
