Fluoromount-G系列荧光封片剂、抗淬灭封片剂


Fluoromount-G系列荧光封片剂、抗淬灭封片剂

简要描述:SouthernBiotech(SBA) Fluoromount-G系列荧光封片剂、抗淬灭封片剂

详细介绍

产品咨询

SouthernBiotechSBA Fluoromount-G系列荧光封片剂、抗淬灭封片剂
 
来自高品质SouthernBiotechSBA
.封片剂
 

 
 
HCC38胸腺癌细胞系,EGFR-FITC染色,DAPI-Fluoromount-G(cat#0100-20)复染及封片.
 
产品特点:
操作简便,用于长时间保存玻片
有一定抗淬灭作用.
适用组织切片、细胞涂片/爬片
产品信息

货号
 

名称
 

规格
 

0100-01

Fluoromount-G
25 mL

封片剂

0100-20

Dapi-Fluoromount-G
20 mL

含核酸染料DAPI,复染和封一次完成

 
 
二.抗淬灭封片剂
 
 
小鼠杂交癌细胞(clone 14-4-4S),羊抗小鼠Ig-FITC染色
A:Anti-Fade-DAPI- Fluoromount-G(cat#0100-40)封片,立刻或在政治部弧灯下暴露5分钟后拍照;
B:Fluoromount-G(cat#0100-01)封片,相同条件下拍照
产品特点
※操作简便,用于长时间保存玻片
※有效抗荧光淬灭
※适用于组织切片、细胞涂片/爬片
产品信息

货号
 

名称
 

规格
 

0100-30

Anti-Fade Fluoromount-G

20 mL

封片剂,可以有效防止FITC和texas Red的淬灭

0100-40

Anti-Fade Dapi-Fluoromount-G

20 mL

含核酸染料DAPI和抗淬灭剂,可以有效防止FITC和Texas Red的淬灭

 
 
 
三.使用及贮存
(一)  使用方法
1.组织切片
1.1免疫染色、脱水、透明;
1.2加1~2滴封片剂覆盖组织,盖上盖玻片。用纱布片轻轻按压,使得多余的封片剂将盖玻片周围封住;
1.3玻片置于空气中干燥5min,检测。
2.细胞涂片/爬片
2.1免疫染色;
2.2固定、再水合;
2.3每次从水性缓冲液中取出一片玻片,加1滴封片剂至细胞,盖上盖玻片。用纱布片轻轻按压使得多余的封片剂将盖玻片周围封住;
2.4玻片置于人气中干5min,检测。
(二)产品状态贮存
※水溶性透明液体,含0.1NaN3
※室温避光贮存

水溶性抗荧光淬灭封片剂(含DAPI)|DAPI Fluoromount-G™

水溶性抗荧光淬灭封片剂(含DAPI)|DAPI Fluoromount-G™

产品说明书

FAQ

COA

已发表文献

产品描述

DAPI Fluoromount-G™是一种含有蓝色细胞核荧光探针-DAPI(Em=455 nm)的水溶性封片剂,用来对最后一步染色是在水溶液体系中完成的玻片样本进行封片,可以显著减缓各种常见荧光染料的荧光淬灭现象。本封片剂具有半永久性封固性,可用作长期保存玻片样本的制作。适用于细胞制备物和组织切片的封片,兼容于免疫荧光,免疫细胞化学,免疫组化等实验。

本品已加DAPI,可同时完成核染和封片,使得实验操作更简便。封片剂干燥后形成一层牢固的涂层,光学透明,便于后续荧光观察和拍照。

运输和保存方法

室温运输。室温避光保存,一年有效。

使用方法
1) 根据实验室已有的体系制备细胞离心涂片;
2) 固定和再水化细胞涂片;
3) 从染色最后一步的水溶性缓冲液中取出一片玻片,直接滴加一滴 DAPI Fluoromount-G™至细胞涂片,盖上盖玻片,用无纺纱布轻轻按压盖玻片以去除多余分封片剂和封固盖玻片。
4) 室温下避光静置5 min,使得封片剂晾干后再进行普通荧光显微镜或者共聚焦显微检测。5) 若玻片样本需长久保存,可使用指甲油或者中性树胶(货号:36313ES60)对盖玻片四周进行密封。

注意事项
1)本品含有0.1%的叠氮钠作为防腐剂,吞食和吸入有毒,操作时请做好防护。
2)为了您的安全和健康,请穿实验服并戴一次性手套操作。
3)本产品仅作科研用途!

Q:该产品需要低温贮藏吗?

A:室温避光保存即可。

Q:使用该产品后多久可进行显微检测?

A:一般室温下避光静置 5 min,使得封片剂晾干后,再进行普通荧光显微镜或者共聚焦显微检测。

Q:该产品可使玻片长久保存吗?

A:不可。若玻片样本需长久保存,可使用指甲油或者中性树胶对盖玻片四周进行密封。

Q:该产品可用于免疫荧光、免疫细胞化学、免疫组化实验吗?

A:可以。

Q:该产品可用于活细胞吗?

A:不推荐,对于染色活细胞膜染料和线粒体染料的抗淬灭效果不佳,建议抗荧光淬灭封片剂用于固定透化的细胞或者组织。

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产品描述

DAPI Fluoromount-G™是一种含有蓝色细胞核荧光探针-DAPI(Em=455 nm)的水溶性封片剂,用来对最后一步染色是在水溶液体系中完成的玻片样本进行封片,可以显著减缓各种常见荧光染料的荧光淬灭现象。本封片剂具有半永久性封固性,可用作长期保存玻片样本的制作。适用于细胞制备物和组织切片的封片,兼容于免疫荧光,免疫细胞化学,免疫组化等实验。

本品已加DAPI,可同时完成核染和封片,使得实验操作更简便。封片剂干燥后形成一层牢固的涂层,光学透明,便于后续荧光观察和拍照。

运输和保存方法

室温运输。室温避光保存,一年有效。

使用方法
1) 根据实验室已有的体系制备细胞离心涂片;
2) 固定和再水化细胞涂片;
3) 从染色最后一步的水溶性缓冲液中取出一片玻片,直接滴加一滴 DAPI Fluoromount-G™至细胞涂片,盖上盖玻片,用无纺纱布轻轻按压盖玻片以去除多余分封片剂和封固盖玻片。
4) 室温下避光静置5 min,使得封片剂晾干后再进行普通荧光显微镜或者共聚焦显微检测。5) 若玻片样本需长久保存,可使用指甲油或者中性树胶(货号:36313ES60)对盖玻片四周进行密封。

注意事项
1)本品含有0.1%的叠氮钠作为防腐剂,吞食和吸入有毒,操作时请做好防护。
2)为了您的安全和健康,请穿实验服并戴一次性手套操作。
3)本产品仅作科研用途!

Q:该产品需要低温贮藏吗?

A:室温避光保存即可。

Q:使用该产品后多久可进行显微检测?

A:一般室温下避光静置 5 min,使得封片剂晾干后,再进行普通荧光显微镜或者共聚焦显微检测。

Q:该产品可使玻片长久保存吗?

A:不可。若玻片样本需长久保存,可使用指甲油或者中性树胶对盖玻片四周进行密封。

Q:该产品可用于免疫荧光、免疫细胞化学、免疫组化实验吗?

A:可以。

Q:该产品可用于活细胞吗?

A:不推荐,对于染色活细胞膜染料和线粒体染料的抗淬灭效果不佳,建议抗荧光淬灭封片剂用于固定透化的细胞或者组织。

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emsdiasum Fluoromount-G荧光封片剂

 

emsdiasum Fluoromount-G荧光封片剂—经典的抗荧光淬灭封片剂

摘要

荧光淬灭(quench)是指荧光分子由内部因素和外部因素同时作用造成的不可逆破坏。内部因素主要是分子从激发态回到基态以非辐射跃迁形式释放能量。外部因素则通常是因为荧光分子在激发光的照射下,经历多次激发/发射过程后,造成分子内部结构发生不可逆的变化,从而导致其不能吸收更多的光子,影响进一步发射荧光。

 

 

荧光的淬灭:荧光淬灭(quench)是指荧光分子由内部因素和外部因素同时作用造成的不可逆破坏。内部因素主要是分子从激发态回到基态以非辐射跃迁形式释放能量。外部因素则通常是因为荧光分子在激发光的照射下,经历多次激发/发射过程后,造成分子内部结构发生不可逆的变化,从而导致其不能吸收更多的光子,影响进一步发射荧光。我们也称之为该分子被光“漂白”了。同时,当激光功率高时,荧光分子有可能在高强度的激光下被“漂白”,造成荧光淬灭现象。需要注意的是,当荧光物质浓度过大时,也可能会产生自淬灭现象。

能够引起荧光淬灭的物质称淬灭剂。如卤素离子、重金属离子、具有氧化性的有机化合物(硝基化合物、重氮化合物、羰基化合物和羟基化合物)及氧分子等。在具体操作实验中,要注意上述淬灭剂对实验可能造成影响。

 

产品名称及描述 品牌 货号
Fluoromount-G荧光封片剂 emsdiasum 17984-25
DAPI-Fluoromount-G荧光封片剂 emsdiasum 17984-24