标签归档：抗兔

647标记山羊抗兔IgG抗体(H+L) 647山羊抗兔IgG抗体|Goat Anti-Rabbit IgG

发表于由

647标记山羊抗兔IgG抗体(H+L) 647山羊抗兔IgG抗体|Goat Anti-Rabbit IgG

产品说明书

FAQ

COA

已发表文献

产品描述

本品是由Alexa Fluor 647标记的山羊抗兔IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。

Alexa Fluor 647的光谱性质类似于APC，其最大激发波长（Amax）为651 nm，最大发射波长（Emax）为667 nm。本品适合做单标实验。要做多标（multiple-labeling）实验，建议使用与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

产品应用

建议稀释浓度：1:50-1:400（For most applications）

产品性质

抗体浓度（Antibody Concentration）

100 µL（0.75 mg/mL）

缓冲液（Buffer）

0.005 M 磷酸钠，0.125 M 氯化钠，pH 7.6

稳定剂（Stabilizer）

7.5 mg/mL BSA（无IgG，蛋白酶），50% 甘油

防腐剂（Preservative）

0.025% 叠氮化钠

原料来源（Source of Material）

Jackson Immunoresearch 111-605-003

运输与保存方法

冰袋运输。-20℃分装保存，尽量避免反复冻融。有效期1年。
 

注意事项

1）本品含叠氮化钠，对人体有害，请注意适当防护。
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
3）本产品仅作科研用途！

 

647标记山羊抗兔IgG抗体(H+L) 647山羊抗兔IgG抗体|Goat Anti-Rabbit IgG

暂无内容

[1] Zhou Y, Ke P, Bao X, et al. Peptide nano-blanket impedes fibroblasts activation and subsequent formation of pre-metastatic niche. Nat Commun. 2022;13(1):2906. Published 2022 May 25. doi:10.1038/s41467-022-30634-8(IF:14.919)
[2] Jin ZR, Fang D, Liu BH, et al. Roles of CatSper channels in the pathogenesis of asthenozoospermia and the therapeutic effects of acupuncture-like treatment on asthenozoospermia. Theranostics. 2021;11(6):2822-2844. Published 2021 Jan 1. doi:10.7150/thno.51869(IF:11.556)
[3] Zhang B, Wang H, Liao Z, et al. EGFP-EGF1-conjugated nanoparticles for targeting both neovascular and glioma cells in therapy of brain glioma. Biomaterials. 2014;35(13):4133-4145. doi:10.1016/j.biomaterials.2014.01.071(IF:8.312)
[4] Luo Z, Jin K, Pang Q, et al. On-Demand Drug Release from Dual-Targeting Small Nanoparticles Triggered by High-Intensity Focused Ultrasound Enhanced Glioblastoma-Targeting Therapy. ACS Appl Mater Interfaces. 2017;9(37):31612-31625. doi:10.1021/acsami.7b10866(IF:7.504)
[5] Wang C, Zhang J, Song S, et al. Imaging epileptic foci in mouse models via a low-density lipoprotein receptor-related protein-1 targeting strategy. EBioMedicine. 2021;63:103156. doi:10.1016/j.ebiom.2020.103156(IF:5.736)
[6] Wang L, Qian Y, Che X, Jiang J, Suo J, Wang Z. Isolation and Characterization of Primary Retinal Microglia From the Human Post-mortem Eyes for Future Studies of Ocular Diseases. Front Cell Neurosci. 2022;15:786020. Published 2022 Jan 13. doi:10.3389/fncel.2021.786020(IF:5.505)
[7] Zhao J, Xie W, Yang Z, et al. Identification and characterization of a special type of subnuclear structure: AGGF1-coated paraspeckles. FASEB J. 2022;36(6):e22366. doi:10.1096/fj.202101690RR(IF:5.192)

产品描述

本品是由Alexa Fluor 647标记的山羊抗兔IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。

Alexa Fluor 647的光谱性质类似于APC，其最大激发波长（Amax）为651 nm，最大发射波长（Emax）为667 nm。本品适合做单标实验。要做多标（multiple-labeling）实验，建议使用与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

产品应用

建议稀释浓度：1:50-1:400（For most applications）

产品性质

抗体浓度（Antibody Concentration）

100 µL（0.75 mg/mL）

缓冲液（Buffer）

0.005 M 磷酸钠，0.125 M 氯化钠，pH 7.6

稳定剂（Stabilizer）

7.5 mg/mL BSA（无IgG，蛋白酶），50% 甘油

防腐剂（Preservative）

0.025% 叠氮化钠

原料来源（Source of Material）

Jackson Immunoresearch 111-605-003

运输与保存方法

冰袋运输。-20℃分装保存，尽量避免反复冻融。有效期1年。
 

注意事项

1）本品含叠氮化钠，对人体有害，请注意适当防护。
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
3）本产品仅作科研用途！

 

647标记山羊抗兔IgG抗体(H+L) 647山羊抗兔IgG抗体|Goat Anti-Rabbit IgG

暂无内容

[1] Zhou Y, Ke P, Bao X, et al. Peptide nano-blanket impedes fibroblasts activation and subsequent formation of pre-metastatic niche. Nat Commun. 2022;13(1):2906. Published 2022 May 25. doi:10.1038/s41467-022-30634-8(IF:14.919)
[2] Jin ZR, Fang D, Liu BH, et al. Roles of CatSper channels in the pathogenesis of asthenozoospermia and the therapeutic effects of acupuncture-like treatment on asthenozoospermia. Theranostics. 2021;11(6):2822-2844. Published 2021 Jan 1. doi:10.7150/thno.51869(IF:11.556)
[3] Zhang B, Wang H, Liao Z, et al. EGFP-EGF1-conjugated nanoparticles for targeting both neovascular and glioma cells in therapy of brain glioma. Biomaterials. 2014;35(13):4133-4145. doi:10.1016/j.biomaterials.2014.01.071(IF:8.312)
[4] Luo Z, Jin K, Pang Q, et al. On-Demand Drug Release from Dual-Targeting Small Nanoparticles Triggered by High-Intensity Focused Ultrasound Enhanced Glioblastoma-Targeting Therapy. ACS Appl Mater Interfaces. 2017;9(37):31612-31625. doi:10.1021/acsami.7b10866(IF:7.504)
[5] Wang C, Zhang J, Song S, et al. Imaging epileptic foci in mouse models via a low-density lipoprotein receptor-related protein-1 targeting strategy. EBioMedicine. 2021;63:103156. doi:10.1016/j.ebiom.2020.103156(IF:5.736)
[6] Wang L, Qian Y, Che X, Jiang J, Suo J, Wang Z. Isolation and Characterization of Primary Retinal Microglia From the Human Post-mortem Eyes for Future Studies of Ocular Diseases. Front Cell Neurosci. 2022;15:786020. Published 2022 Jan 13. doi:10.3389/fncel.2021.786020(IF:5.505)
[7] Zhao J, Xie W, Yang Z, et al. Identification and characterization of a special type of subnuclear structure: AGGF1-coated paraspeckles. FASEB J. 2022;36(6):e22366. doi:10.1096/fj.202101690RR(IF:5.192)

488标记山羊抗兔IgG抗体(H+L)F(ab)'2片段|Goat Anti-Rabbit IgG

发表于由

488标记山羊抗兔IgG抗体(H+L)F(ab)'2片段|Goat Anti-Rabbit IgG

产品说明书

FAQ

COA

已发表文献

产品简介

本品是由Alexa Fluor® 488标记的山羊抗兔IgG(H+L) F(ab') 2，是将山羊抗血清经胃蛋白酶消化并使用抗原偶联的琼脂糖微珠亲和色谱纯化所得，借此可去除Fc片段和完整IgG分子。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。本品预先与相近物种包括人、小鼠、大鼠的血清蛋白经过亲和吸附处理，ELISA和/或固相免疫吸附检测证实本品与以上物种几乎无交叉反应。但可能会与其他物种免疫球蛋白发生交叉反应。本品不会与非免疫球蛋白类的血清蛋白发生交叉反应。

Alexa Fluor® 488的光谱性质同FITC，Amax(最大激发波长)为493 nm，Emax(最大发射波长)为519 nm。本品适合做单标实验，广泛应用于多种免疫学实验如免疫细胞化学（ICC），流式细胞术（FC）等。要做多标（Multiple-labeling）实验，建议使用经过与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

 

产品应用

建议稀释浓度：1:100-1:800（For most application）

 

产品信息

抗体浓度（Antibody Concentration）

100 μL（0.75 mg/mL）

缓冲液（Buffer）

0.005M 磷酸钠，0.125M 氯化钠，pH7.6

稳定剂（Stabilizer）

7.5 mg/mL BSA（无IgG，蛋白酶)， 50%甘油

防腐剂（Preservative）

0.025%叠氮化钠

原料来源（Source of Material）

Jackson Immunoresearch 111-546-144

 

储存条件

-25 ~ -15℃分装保存，尽量避免反复冻融。有效期1年。

 

注意事项

1.本品含叠氮化钠，对人体有害，请注意适当防护。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

 

488标记山羊抗兔IgG抗体(H+L)F(ab)'2片段|Goat Anti-Rabbit IgG

暂无内容

[1] He XL, Yang L, Wang ZJ, Huang RQ, Zhu RR, Cheng LM. Solid lipid nanoparticles loading with curcumin and dexanabinol to treat major depressive disorder. Neural Regen Res. 2021;16(3):537-542. doi:10.4103/1673-5374.293155(IF:5.135)
[2] Wei W, Zhang Y, Zhang D, et al. Annotating BCMA Expression in Multiple Myelomas [published online ahead of print, 2021 Nov 29]. Mol Pharm. 2021;10.1021/acs.molpharmaceut.1c00628. doi:10.1021/acs.molpharmaceut.1c00628(IF:4.939)
[3] Li T, Bai J, Du Y, et al. Thiamine pretreatment improves endotoxemia-related liver injury and cholestatic complications by regulating galactose metabolism and inhibiting macrophage activation. Int Immunopharmacol. 2022;108:108892. doi:10.1016/j.intimp.2022.108892(IF:4.932)
[4] Yang X, Zou R, Dai X, Wu X, Yuan F, Feng Y. YAP is critical to inflammation, endothelial-mesenchymal transition and subretinal fibrosis in experimental choroidal neovascularization. Exp Cell Res. 2022;417(2):113221. doi:10.1016/j.yexcr.2022.113221(IF:3.905)

产品简介

本品是由Alexa Fluor® 488标记的山羊抗兔IgG(H+L) F(ab') 2，是将山羊抗血清经胃蛋白酶消化并使用抗原偶联的琼脂糖微珠亲和色谱纯化所得，借此可去除Fc片段和完整IgG分子。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。本品预先与相近物种包括人、小鼠、大鼠的血清蛋白经过亲和吸附处理，ELISA和/或固相免疫吸附检测证实本品与以上物种几乎无交叉反应。但可能会与其他物种免疫球蛋白发生交叉反应。本品不会与非免疫球蛋白类的血清蛋白发生交叉反应。

Alexa Fluor® 488的光谱性质同FITC，Amax(最大激发波长)为493 nm，Emax(最大发射波长)为519 nm。本品适合做单标实验，广泛应用于多种免疫学实验如免疫细胞化学（ICC），流式细胞术（FC）等。要做多标（Multiple-labeling）实验，建议使用经过与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

 

产品应用

建议稀释浓度：1:100-1:800（For most application）

 

产品信息

抗体浓度（Antibody Concentration）

100 μL（0.75 mg/mL）

缓冲液（Buffer）

0.005M 磷酸钠，0.125M 氯化钠，pH7.6

稳定剂（Stabilizer）

7.5 mg/mL BSA（无IgG，蛋白酶)， 50%甘油

防腐剂（Preservative）

0.025%叠氮化钠

原料来源（Source of Material）

Jackson Immunoresearch 111-546-144

 

储存条件

-25 ~ -15℃分装保存，尽量避免反复冻融。有效期1年。

 

注意事项

1.本品含叠氮化钠，对人体有害，请注意适当防护。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

 

488标记山羊抗兔IgG抗体(H+L)F(ab)'2片段|Goat Anti-Rabbit IgG

暂无内容

[1] He XL, Yang L, Wang ZJ, Huang RQ, Zhu RR, Cheng LM. Solid lipid nanoparticles loading with curcumin and dexanabinol to treat major depressive disorder. Neural Regen Res. 2021;16(3):537-542. doi:10.4103/1673-5374.293155(IF:5.135)
[2] Wei W, Zhang Y, Zhang D, et al. Annotating BCMA Expression in Multiple Myelomas [published online ahead of print, 2021 Nov 29]. Mol Pharm. 2021;10.1021/acs.molpharmaceut.1c00628. doi:10.1021/acs.molpharmaceut.1c00628(IF:4.939)
[3] Li T, Bai J, Du Y, et al. Thiamine pretreatment improves endotoxemia-related liver injury and cholestatic complications by regulating galactose metabolism and inhibiting macrophage activation. Int Immunopharmacol. 2022;108:108892. doi:10.1016/j.intimp.2022.108892(IF:4.932)
[4] Yang X, Zou R, Dai X, Wu X, Yuan F, Feng Y. YAP is critical to inflammation, endothelial-mesenchymal transition and subretinal fibrosis in experimental choroidal neovascularization. Exp Cell Res. 2022;417(2):113221. doi:10.1016/j.yexcr.2022.113221(IF:3.905)

生物素高效标记山羊抗兔IgG(H+L)(A0279)

发表于由
生物素高效标记山羊抗兔IgG(H+L)
生物素高效标记山羊抗兔IgG(H+L)(A0279) 一键复制产品信息

产品编号: A0279

产品包装:0.5ml
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0.5ml

数
量
件

说明书下载

262.00 253.00 10

价格: ¥ 262.00

促销价: ¥ 262.00

促销价: ¥ 262.00 253.00

产品简介
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产品编号 产品名称 产品包装 产品价格
A0279 生物素高效标记山羊抗兔IgG(H+L) 0.5ml 262.00元

来源 用途 WB ICC/IF IHC ELISA
Goat WB, ICC, IF, IHC, ELISA 1:500-5000 1:50-200 1:50-200 1:2000-30000

WB, Western blot; ICC, Immunocytochemistry; IF, Immunofluorescence; IHC, Immunohistochemistry; ELISA, Enzyme-linked immune-sorbent assay.
本生物素高效标记山羊抗兔IgG(H+L) (Biotin-labeled Goat Anti-Rabbit IgG(H+L) with high mole ratio)为进口分装，用于Western、免疫细胞化学、免疫荧光、免疫组化和ELISA等检测的高纯度高特异性二抗。
生物素标记后可以采用SABC(Streptavidin-Biotin Complex)法进行检测；也可以使用辣根过氧化物酶标记的Streptavidin或碱性磷酸酯酶标记的Streptavidin来识别生物素，后续用辣根过氧化物酶或碱性磷酸酯酶对应的检测体系进行显色反应或发光反应等。
本抗体为用纯化的兔IgG(H+L)免疫山羊，然后用亲和纯化柱对获得的抗血清进行纯化，并经过小鼠IgG等吸附纯化和生物标记的优质二抗。对小鼠等的血清蛋白几乎没有结合能力(Minimal Cross-reaction to Mouse)。
本生物素高效标记山羊抗兔IgG(H+L)每摩尔IgG结合多摩尔的生物素，能结合多个streptavidin或avidin分子，在SABC或ABC等检测系统中，能有效放大信号，提高检测灵敏度。
本生物素高效标记山羊抗兔IgG(H+L)用于Western、免疫细胞化学、免疫荧光、免疫组化和ELISA的推荐稀释比例参考上表，实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。
本抗体如果用于常规的Western检测，以每次检测需10毫升1:2000稀释的二抗计算，可以检测100次；如果用于常规的免疫细胞化学或免疫组化检测，按照每样需要100微升1:100稀释的本抗体计算，可以检测500个样品。
包装清单：

产品编号 产品名称 包装
A0279 生物素高效标记山羊抗兔IgG(H+L) 0.5ml
— 说明书 1份

保存条件：
-20℃保存，一年有效。
注意事项：
 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。 

使用说明：
1.Western、免疫细胞化学、免疫荧光、免疫组化和ELISA请参考相关实验步骤进行。起始稀释浓度请按照产品简介中推荐的稀释比例进行稀释。

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生物素高效标记山羊抗兔IgG(H+L)(A0279)
生物素高效标记山羊抗兔IgG(H+L)(A0279)

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生物素高效标记山羊抗兔IgG(H+L)(A0279)

生物素高效标记山羊抗兔IgG(H+L)(A0279)
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生物素标记山羊抗兔IgG(H+L)(A0277)

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生物素标记山羊抗兔IgG(H+L)
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产品编号: A0277

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促销价: ¥ 350.00 338.00

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产品编号 产品名称 产品包装 产品价格
A0277 生物素标记山羊抗兔IgG(H+L) 1ml 350.00元

来源 用途 WB/ELISA IHC/IC IF/F
Goat WB, ELISA, IHC, IC, IF, F 1:1000-3000 1:50-500 1:25-250

WB, Western blot; IHC,Immunohistochemistry; IC,Immunocytochemistry; IF,Immunofluorescence; F,Flow cytometry。
本生物素标记山羊抗兔IgG(H+L)(Biotin-labeled Goat Anti-Rabbit IgG(H+L))为进口分装，用于Western、ELISA、免疫组化、免疫细胞化学、免疫荧光和流式细胞仪分析的高纯度高特异性二抗。
生物素标记后可以采用辣根过氧化物酶标记的Streptavidin或碱性磷酸酯酶标记的Streptavidin进行后续的检测。随后用辣根过氧化物酶或碱性磷酸酯酶对应的检测体系进行显色反应或发光反应等。
本抗体为用纯化的兔IgG免疫山羊，然后用亲和纯化柱对获得的抗血清进行纯化，并经过人IgG、小鼠IgG和大鼠IgG吸附纯化的优质二抗。对人、小鼠和大鼠的血清蛋白几乎没有结合能力(Minimal Cross-reaction to Human, Mouse and Rat Serum Proteins)。
本生物素标记山羊抗兔IgG(H+L)用于Western、ELISA、免疫组化、免疫细胞化学、免疫荧光和流式细胞仪分析的推荐稀释比例参考上表，实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。
本抗体如果用于常规的Western检测，以每次检测需10毫升1:1000稀释的二抗计，可以检测100次。
包装清单：

产品编号 产品名称 包装
A0277 生物素标记山羊抗兔IgG (H+L) 1ml
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保存条件：
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注意事项：
 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. Western、ELISA、免疫组化、免疫细胞化学、免疫荧光和流式细胞仪分析请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。

生物素标记山羊抗兔IgG(H+L)(A0277)
生物素标记山羊抗兔IgG(H+L)(A0277)

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生物素标记山羊抗兔IgG(H+L)(A0277)

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碱性磷酸酯酶标记山羊抗兔IgG(H+L)(A0239)

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碱性磷酸酯酶标记山羊抗兔IgG(H+L)
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产品编号: A0239

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282.00 272.00 10

价格: ¥ 282.00

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促销价: ¥ 282.00 272.00

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A0239 碱性磷酸酯酶标记山羊抗兔IgG(H+L) 0.5ml 282.00元

来源 用途 WB ELISA IHC
Goat WB, ELISA, IHC 1:500-5000 1:500-5000 1:500-2000

WB, Western blot; IHC, Immunohistochemistry.
本碱性磷酸酯酶标记山羊抗兔IgG (H+L) (AP-labeled Goat Anti-Rabbit IgG (H+L))为进口分装，用于Western、ELISA和IHC等的高纯度高特异性二抗。
碱性磷酸酯酶(Alkaline Phosphatase, AP/ALP/AKP/ALKP/ALPase/Alk Phos)常被称作碱性磷酸酶(EC 3.1.3.1)，是一类水解酶，通过水解磷酸单酯将底物分子上的磷酸基团除去，并生成磷酸根离子和自由的羟基，其去磷酸化作用的底物包括蛋白质、核苷酸和生物碱等，并在碱性条件下最为有效。该酶是一组同功酶的统称。常见的小牛肠碱性磷酸酶(Calf Intestinal Alkaline Phosphatase, CIAP/CIP)被广泛用于二抗等的标记最终用于蛋白和核酸等的检测，例如在Western、ELISA、IHC、ICC、Northern、Southern等时，催化BCIP/NBT等显色试剂显色或催化适当的化学发光试剂产生化学发光。本产品标记的就是小牛肠碱性磷酸酶。
本抗体为用纯化的兔IgG免疫山羊，然后用亲和纯化柱对获得的抗血清进行纯化，并经过人、小鼠和大鼠IgG吸附纯化的优质二抗。对人、小鼠和大鼠IgG几乎没有结合能力(Minimal Cross-reaction to Human, Mouse and Rat Serum Proteins)。
本碱性磷酸酯酶标记山羊抗兔IgG (H+L)用于WB、ELISA和IHC的推荐稀释比例参考上表，实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。
本抗体如果用于常规的Western检测，以每次检测需10毫升1:1000稀释的二抗计，可以检测50次。
包装清单：

产品编号 产品名称 包装
A0239 碱性磷酸酯酶标记山羊抗兔IgG (H+L) 0.5ml
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保存条件：
-20℃保存，一年有效。
注意事项：
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. Western、ELISA或IHC请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。

碱性磷酸酯酶标记山羊抗兔IgG(H+L)(A0239)
碱性磷酸酯酶标记山羊抗兔IgG(H+L)(A0239)

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碱性磷酸酯酶标记山羊抗兔IgG(H+L)(A0239)

碱性磷酸酯酶标记山羊抗兔IgG(H+L)(A0239)
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