标签归档：噻唑

MTT噻唑兰 溴化噻唑蓝四氮唑|MTT Thiazolam

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MTT噻唑兰 溴化噻唑蓝四氮唑|MTT Thiazolam

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产品描述

 

噻唑兰（Methylthiazolyldiphenyl–tetrazolium bromide, MTT），也称作溴化噻唑蓝四氮唑，是一种黄色染料，已经普遍替代传统的台盼蓝染色法或者放射性同位素插入法，用来检测细胞的活力，细胞增殖以及细胞毒性分析。检测原理在于：MTT是一种可接受氢离子的化合物染料，带正电荷，具有细胞膜渗透性。活细胞线粒体中的琥珀酸脱氢酶能够将外源进入的MTT还原成为水不溶性的深蓝色MTT-甲臜结晶，而死细胞不具有此功能。甲臜结晶是不能穿透细胞膜，因此沉积在处于增殖且未受损伤的细胞内。甲臜结晶可用DMSO或者酸化的异丙醇溶解出来，酶标仪下测定570 nm的吸光度反映甲臜的生成量。而甲臜的生成量与活细胞数目成正比，用以评估细胞存活状况。

MTT也可用作免疫组化/细胞化学染色试剂，也可用来检测NAD。MTT被快速还原成甲臜后，能够螯合镍，铜和钴。钴螯合物可参与机体氧化系统。

 

产品性质

 

中文别名（Chinese synonym）

溴化噻唑蓝四氮唑；

3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四氮唑噻唑蓝；

英文别名（English synonym）

 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium Bromide;

Methylthiazolyldiphenyl–tetrazolium bromide;

Thiazoyl blue tetrazolium bromide;

CAS号（CAS NO.）

298-93-1

分子式（Formula）

C18H16N5SBr

外观（Appearance）

淡黄色至橙色粉末

分子量（Molecular weight）

414.3

纯度（purity）

≥98%

熔点（Melting point）

187℃

溶解性（Solubility）

溶于水，甲醇

结构式（Structure）

MTT噻唑兰 溴化噻唑蓝四氮唑|MTT Thiazolam 

 

运输和保存方法

 

冰袋运输。4℃避光干燥保存，有效期2年。

 

注意事项

 

1）溶解甲臜的有机溶剂具有毒性，使用时注意防护。

2）MTT有致癌性，应小心操作；MTT溶液需要无菌，因其对菌很敏感。MTT溶液不可4℃保存超过1周，因其可能发生降解，导致检测结果错误。

3）MTT法只能用来检测细胞相对数和相对活力，但不能测定细胞绝对数。在用酶标仪检测结果的时候，为了保证实验结果的线性，MTT吸光度最好在0-0.7范围内。

4）为了您的安全和健康，请穿实验服并戴一次性手套操作。

5）本产品仅作科研用途！

 

使用方法（以96孔板为例）

 

细胞生长的培养条件会影响检测结果，因此进行MTT检测时需考虑这些条件，包括：培养时间，传代次数以及生长培养基的成分等。一般情况，48-72 h培养时间下，最初铺板的细胞密度可控制在5000-10,000/孔。

【注】：最终检测的培养液内含有酚红会严重影响检测结果。推荐MTT检测时细胞培养在无酚红环境下。也可以在进行MTT孵育的时候改用无酚红培养液。

A．工作液配制

1） MTT溶液

生化研究中，常配制成5 mg/mL的储备液。MTT最好现配现用，可以称取MTT 0.5 g，溶于100 mL的磷酸缓冲液（PBS）或无酚红的培养基中，用0.22 μm滤膜过滤以除去溶液里的细菌，4℃ 避光短时间保存。建议小剂量分装放到-20℃长期保存，避免反复冻融。

2） SDS-HCl溶液（MTT终止液）

取10 mL 0.01 M HCl加入1 g SDS，颠倒混匀或者超声使其完全溶解，此时即为终止液。建议现配现用。

B．MTT细胞增殖检测

1）接种细胞：用含10%胎牛血清（FBS）的培养液配制成单个细胞悬液，调整细胞密度，以每孔5000-10, 000个细胞接种到96孔板，每孔体积100 µL，培养板的边缘孔用无菌PBS填充。设置空白对照孔（不含有细胞）。

2）37℃，5% CO2，培养24-72 h。

3）可选：对于贴壁细胞，去除旧的培养液，更换100 µL/孔新鲜的培养液（不含酚红）；对于悬浮细胞，离心96孔板，去除尽可能多的上清液。然后加入 100 µL/孔新鲜的培养液（不含酚红）重悬细胞；

4）每孔加入10 µL MTT溶液（5 mg/mL），加样时尽量勤换枪头，控制加量误差。

5）水平摇床上低速混匀1 min后，放入37℃培养箱孵育4h。【注】：对于高密度的细胞（>100,000 cells/孔）可缩短孵育时间至2 h。

6）对于贴壁细胞，直接吸掉旧的培养液，避免枪尖刮破单细胞层。对于悬浮细胞，离心收集细胞，去除培养液。

7）加入100 µL/孔SDS-HCl溶液，用枪充分混匀后放入37℃培养箱孵育4 h。【注】：更长的孵育时间会降低检测灵敏度。

8）孵育结束，放置在酶标仪上摇匀后，选择570 nm，测定吸光度。记录结果，比色以空白调零。

【注】：对于细胞毒性检测，以未经药物处理细胞的OD值为100%，然后计算药物处理细胞所占的比率（x），公式如下：OD（未处理细胞）/OD（药物处理细胞）=100%/x。然后建立直方图。

 

HB211228

MTT噻唑兰 溴化噻唑蓝四氮唑|MTT Thiazolam

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[1] Wang Y, Zhang G, Meng Q, et al. Precise tumor immune rewiring via synthetic CRISPRa circuits gated by concurrent gain/loss of transcription factors. Nat Commun. 2022;13(1):1454. Published 2022 Mar 18. doi:10.1038/s41467-022-29120-y(IF:14.919)
[2] Zhao H, Xu J, Huang W, et al. Spatiotemporally Light-Activatable Platinum Nanocomplexes for Selective and Cooperative Cancer Therapy. ACS Nano. 2019;13(6):6647-6661. doi:10.1021/acsnano.9b00972(IF:13.903)
[3] Mugaka BP, Zhang S, Li RQ, et al. One-Pot Preparation of Peptide-Doped Metal-Amino Acid Framework for General Encapsulation and Targeted Delivery. ACS Appl Mater Interfaces. 2021;13(9):11195-11204. doi:10.1021/acsami.0c22194(IF:9.229)
[4] Deng X, Ye D, Hua K, et al. MIR22HG inhibits breast cancer progression by stabilizing LATS2 tumor suppressor. Cell Death Dis. 2021;12(9):810. Published 2021 Aug 26. doi:10.1038/s41419-021-04105-9(IF:8.469)
[5] Zhou B, Fang L, Dong Y, et al. Mitochondrial quality control protects photoreceptors against oxidative stress in the H2O2-induced models of retinal degeneration diseases. Cell Death Dis. 2021;12(5):413. Published 2021 Apr 20. doi:10.1038/s41419-021-03660-5(IF:8.469)
[6] Han H, Jin Q, Wang H, et al. Intracellular Dual Fluorescent Lightup Bioprobes for Image-Guided Photodynamic Cancer Therapy. Small. 2016;12(28):3870-3878. doi:10.1002/smll.201600950(IF:8.315)
[7] Fan X, Ke L, Cheng H, et al. Enhanced drug retention by anthracene crosslinked nanocomposites for bimodal imaging-guided phototherapy. Nanoscale. 2021;13(35):14713-14722. Published 2021 Sep 17. doi:10.1039/d1nr04171a(IF:7.790)
[8] Lv N, Jin S, Liang Z, et al. PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway. Front Immunol. 2022;12:751409. Published 2022 Jan 7. doi:10.3389/fimmu.2021.751409(IF:7.561)
[9] Li H, Li X, Wang Y, Ji J. Introduction of lactobionic acid ligand into mixed-charge nanoparticles to realize in situ triggered active targeting to hepatoma cells. Mater Today Bio. 2019;4:100034. Published 2019 Oct 25. doi:10.1016/j.mtbio.2019.100034(IF:7.348)
[10] Yan P, Shu X, Zhong H, et al. A versatile nanoagent for multimodal imaging-guided photothermal and anti-inflammatory combination cancer therapy. Biomater Sci. 2021;9(14):5025-5034. doi:10.1039/d1bm00576f(IF:6.843)
[11] Jiang D, Xu M, Pei Y, et al. Core-matched nanoassemblies for targeted co-delivery of chemotherapy and photosensitizer to treat drug-resistant cancer. Acta Biomater. 2019;88:406-421. doi:10.1016/j.actbio.2019.02.009(IF:6.638)
[12] Yu Y, Chen J, Liu S, Cheng D. ROS-responsive organosilica nanocarrier for the targeted delivery of metformin against cancer with the synergistic effect of hypoglycemia. J Mater Chem B. 2021;9(30):6044-6055. doi:10.1039/d1tb01143j(IF:6.331)
[13] Liu A, Wang H, Hou X, et al. Combinatory antitumor therapy by cascade targeting of a single drug. Acta Pharm Sin B. 2020;10(4):667-679. doi:10.1016/j.apsb.2019.08.011(IF:5.808)
[14] Wang X, Song H, Fang L, Wu T. EIF4A3-mediated circPRKCI expression promotes triple-negative breast cancer progression by regulating WBP2 and PI3K/AKT signaling pathway. Cell Death Discov. 2022;8(1):92. Published 2022 Mar 2. doi:10.1038/s41420-022-00892-y(IF:5.241)
[15] Huang Y, Zheng W, Ji C, et al. Circular RNA circRPPH1 promotes breast cancer progression via circRPPH1-miR-512-5p-STAT1 axis. Cell Death Discov. 2021;7(1):376. Published 2021 Dec 6. doi:10.1038/s41420-021-00771-y(IF:5.241)
[16] Chen J, Huang Y, Hu X, Bian X, Nian S. Gastrodin prevents homocysteine-induced human umbilical vein endothelial cells injury via PI3K/Akt/eNOS and Nrf2/ARE pathway. J Cell Mol Med. 2021;25(1):345-357. doi:10.1111/jcmm.16073(IF:4.486)
[17] Cheng X, Li D, Qi T, Sun J, Zhou T, Zheng WV. Objective to identify and verify the regulatory mechanism of DTNBP1 as a prognostic marker for hepatocellular carcinoma. Sci Rep. 2022;12(1):211. Published 2022 Jan 7. doi:10.1038/s41598-021-04055-4(IF:4.380)
[18] Tian R, Wang Z, Niu R, Wang H, Guan W, Chang J. Tumor Exosome Mimicking Nanoparticles for Tumor Combinatorial Chemo-Photothermal Therapy. Front Bioeng Biotechnol. 2020;8:1010. Published 2020 Aug 31. doi:10.3389/fbioe.2020.01010(IF:3.644)
[19] Wei B, Wang Z, Lian Q, Chi B, Ma S. hsa_circ_0139402 Promotes Bladder Cancer Progression by Regulating hsa-miR-326/PAX8 Signaling. Dis Markers. 2022;2022:9899548. Published 2022 Feb 1. doi:10.1155/2022/9899548(IF:3.434)
[20] Cui Z, Li Y, Liu G, Jiang Y. miR-103a-3p Silencing Ameliorates Calcium Oxalate Deposition in Rat Kidney by Activating the UMOD/TRPV5 Axis. Dis Markers. 2022;2022:2602717. Published 2022 Feb 23. doi:10.1155/2022/2602717(IF:3.434)
[21] Feng H, Tang J, Zhang P, Miao Y, Wu T, Cheng Z. Anti-adipogenic 18,19-seco-ursane stereoisomers and oleane-type saponins from Ilex cornuta leaves. Phytochemistry. 2020;175:112363. doi:10.1016/j.phytochem.2020.112363(IF:3.044)
[22] Zhu D, Chen C, Xia Y, Kong LY, Luo J. A Purified Resin Glycoside Fraction from Pharbitidis Semen Induces Paraptosis by Activating Chloride Intracellular Channel-1 in Human Colon Cancer Cells. Integr Cancer Ther. 2019;18:1534735418822120. doi:10.1177/1534735418822120(IF:2.634)
[23] Liu Y, Chen G, Wang B, Wu H, Zhang Y, Ye H. Silencing circRNA protein kinase C iota (circ-PRKCI) suppresses cell progression and glycolysis of human papillary thyroid cancer through circ-PRKCI/miR-335/E2F3 ceRNA axis. Endocr J. 2021;68(6):713-727. doi:10.1507/endocrj.EJ20-0726(IF:2.349)
[24] Zhu M, Wu Y, Wang Z, et al. miR-128-3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D. Oncol Lett. 2021;21(2):152. doi:10.3892/ol.2020.12413(IF:2.311)
[25] Chen L, Wu G, Li Y, Cai Q. Anesthetic propofol suppresses growth and metastasis of lung adenocarcinoma in vitro through downregulating circ-MEMO1-miR-485-3p-NEK4 ceRNA axis [published online ahead of print, 2022 May 6]. Histol Histopathol. 2022;18465. doi:10.14670/HH-18-465(IF:2.303)
[26] Mo Q, Liu L, Bao G, Li T. Effects of melanoma differentiation associated gene-7 (MDA-7/IL-24) on apoptosis of liver cancer cells via regulating the expression of B-cell lymphoma-2. Oncol Lett. 2019;18(1):29-34. doi:10.3892/ol.2019.10298(IF:1.871)
[27] Mu J, Wang H, Wang X, Sun P. Expression of miR-124 in gastric adenocarcinoma and the effect on proliferation and invasion of gastric adenocarcinoma SCG-7901 cells. Oncol Lett. 2019;17(3):3406-3410. doi:10.3892/ol.2019.9981(IF:1.871)
[28] Zhou B, Zhao G, Zhu Y, et al. Protective Effects of Nicotinamide Riboside on H2O2-induced Oxidative Damage in Lens Epithelial Cells. Curr Eye Res. 2021;46(7):961-970. doi:10.1080/02713683.2020.1855662(IF:1.754)
[29] Li Y, Zhao Z, Sun D, Li Y. Novel long noncoding RNA LINC02323 promotes cell growth and migration of ovarian cancer via TGF-β receptor 1 by miR-1343-3p. J Clin Lab Anal. 2021;35(2):e23651. doi:10.1002/jcla.23651(IF:1.540)

产品描述

 

噻唑兰（Methylthiazolyldiphenyl–tetrazolium bromide, MTT），也称作溴化噻唑蓝四氮唑，是一种黄色染料，已经普遍替代传统的台盼蓝染色法或者放射性同位素插入法，用来检测细胞的活力，细胞增殖以及细胞毒性分析。检测原理在于：MTT是一种可接受氢离子的化合物染料，带正电荷，具有细胞膜渗透性。活细胞线粒体中的琥珀酸脱氢酶能够将外源进入的MTT还原成为水不溶性的深蓝色MTT-甲臜结晶，而死细胞不具有此功能。甲臜结晶是不能穿透细胞膜，因此沉积在处于增殖且未受损伤的细胞内。甲臜结晶可用DMSO或者酸化的异丙醇溶解出来，酶标仪下测定570 nm的吸光度反映甲臜的生成量。而甲臜的生成量与活细胞数目成正比，用以评估细胞存活状况。

MTT也可用作免疫组化/细胞化学染色试剂，也可用来检测NAD。MTT被快速还原成甲臜后，能够螯合镍，铜和钴。钴螯合物可参与机体氧化系统。

 

产品性质

 

中文别名（Chinese synonym）

溴化噻唑蓝四氮唑；

3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四氮唑噻唑蓝；

英文别名（English synonym）

 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium Bromide;

Methylthiazolyldiphenyl–tetrazolium bromide;

Thiazoyl blue tetrazolium bromide;

CAS号（CAS NO.）

298-93-1

分子式（Formula）

C18H16N5SBr

外观（Appearance）

淡黄色至橙色粉末

分子量（Molecular weight）

414.3

纯度（purity）

≥98%

熔点（Melting point）

187℃

溶解性（Solubility）

溶于水，甲醇

结构式（Structure）

MTT噻唑兰 溴化噻唑蓝四氮唑|MTT Thiazolam 

 

运输和保存方法

 

冰袋运输。4℃避光干燥保存，有效期2年。

 

注意事项

 

1）溶解甲臜的有机溶剂具有毒性，使用时注意防护。

2）MTT有致癌性，应小心操作；MTT溶液需要无菌，因其对菌很敏感。MTT溶液不可4℃保存超过1周，因其可能发生降解，导致检测结果错误。

3）MTT法只能用来检测细胞相对数和相对活力，但不能测定细胞绝对数。在用酶标仪检测结果的时候，为了保证实验结果的线性，MTT吸光度最好在0-0.7范围内。

4）为了您的安全和健康，请穿实验服并戴一次性手套操作。

5）本产品仅作科研用途！

 

使用方法（以96孔板为例）

 

细胞生长的培养条件会影响检测结果，因此进行MTT检测时需考虑这些条件，包括：培养时间，传代次数以及生长培养基的成分等。一般情况，48-72 h培养时间下，最初铺板的细胞密度可控制在5000-10,000/孔。

【注】：最终检测的培养液内含有酚红会严重影响检测结果。推荐MTT检测时细胞培养在无酚红环境下。也可以在进行MTT孵育的时候改用无酚红培养液。

A．工作液配制

1） MTT溶液

生化研究中，常配制成5 mg/mL的储备液。MTT最好现配现用，可以称取MTT 0.5 g，溶于100 mL的磷酸缓冲液（PBS）或无酚红的培养基中，用0.22 μm滤膜过滤以除去溶液里的细菌，4℃ 避光短时间保存。建议小剂量分装放到-20℃长期保存，避免反复冻融。

2） SDS-HCl溶液（MTT终止液）

取10 mL 0.01 M HCl加入1 g SDS，颠倒混匀或者超声使其完全溶解，此时即为终止液。建议现配现用。

B．MTT细胞增殖检测

1）接种细胞：用含10%胎牛血清（FBS）的培养液配制成单个细胞悬液，调整细胞密度，以每孔5000-10, 000个细胞接种到96孔板，每孔体积100 µL，培养板的边缘孔用无菌PBS填充。设置空白对照孔（不含有细胞）。

2）37℃，5% CO2，培养24-72 h。

3）可选：对于贴壁细胞，去除旧的培养液，更换100 µL/孔新鲜的培养液（不含酚红）；对于悬浮细胞，离心96孔板，去除尽可能多的上清液。然后加入 100 µL/孔新鲜的培养液（不含酚红）重悬细胞；

4）每孔加入10 µL MTT溶液（5 mg/mL），加样时尽量勤换枪头，控制加量误差。

5）水平摇床上低速混匀1 min后，放入37℃培养箱孵育4h。【注】：对于高密度的细胞（>100,000 cells/孔）可缩短孵育时间至2 h。

6）对于贴壁细胞，直接吸掉旧的培养液，避免枪尖刮破单细胞层。对于悬浮细胞，离心收集细胞，去除培养液。

7）加入100 µL/孔SDS-HCl溶液，用枪充分混匀后放入37℃培养箱孵育4 h。【注】：更长的孵育时间会降低检测灵敏度。

8）孵育结束，放置在酶标仪上摇匀后，选择570 nm，测定吸光度。记录结果，比色以空白调零。

【注】：对于细胞毒性检测，以未经药物处理细胞的OD值为100%，然后计算药物处理细胞所占的比率（x），公式如下：OD（未处理细胞）/OD（药物处理细胞）=100%/x。然后建立直方图。

 

HB211228

MTT噻唑兰 溴化噻唑蓝四氮唑|MTT Thiazolam

暂无内容

[1] Wang Y, Zhang G, Meng Q, et al. Precise tumor immune rewiring via synthetic CRISPRa circuits gated by concurrent gain/loss of transcription factors. Nat Commun. 2022;13(1):1454. Published 2022 Mar 18. doi:10.1038/s41467-022-29120-y(IF:14.919)
[2] Zhao H, Xu J, Huang W, et al. Spatiotemporally Light-Activatable Platinum Nanocomplexes for Selective and Cooperative Cancer Therapy. ACS Nano. 2019;13(6):6647-6661. doi:10.1021/acsnano.9b00972(IF:13.903)
[3] Mugaka BP, Zhang S, Li RQ, et al. One-Pot Preparation of Peptide-Doped Metal-Amino Acid Framework for General Encapsulation and Targeted Delivery. ACS Appl Mater Interfaces. 2021;13(9):11195-11204. doi:10.1021/acsami.0c22194(IF:9.229)
[4] Deng X, Ye D, Hua K, et al. MIR22HG inhibits breast cancer progression by stabilizing LATS2 tumor suppressor. Cell Death Dis. 2021;12(9):810. Published 2021 Aug 26. doi:10.1038/s41419-021-04105-9(IF:8.469)
[5] Zhou B, Fang L, Dong Y, et al. Mitochondrial quality control protects photoreceptors against oxidative stress in the H2O2-induced models of retinal degeneration diseases. Cell Death Dis. 2021;12(5):413. Published 2021 Apr 20. doi:10.1038/s41419-021-03660-5(IF:8.469)
[6] Han H, Jin Q, Wang H, et al. Intracellular Dual Fluorescent Lightup Bioprobes for Image-Guided Photodynamic Cancer Therapy. Small. 2016;12(28):3870-3878. doi:10.1002/smll.201600950(IF:8.315)
[7] Fan X, Ke L, Cheng H, et al. Enhanced drug retention by anthracene crosslinked nanocomposites for bimodal imaging-guided phototherapy. Nanoscale. 2021;13(35):14713-14722. Published 2021 Sep 17. doi:10.1039/d1nr04171a(IF:7.790)
[8] Lv N, Jin S, Liang Z, et al. PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway. Front Immunol. 2022;12:751409. Published 2022 Jan 7. doi:10.3389/fimmu.2021.751409(IF:7.561)
[9] Li H, Li X, Wang Y, Ji J. Introduction of lactobionic acid ligand into mixed-charge nanoparticles to realize in situ triggered active targeting to hepatoma cells. Mater Today Bio. 2019;4:100034. Published 2019 Oct 25. doi:10.1016/j.mtbio.2019.100034(IF:7.348)
[10] Yan P, Shu X, Zhong H, et al. A versatile nanoagent for multimodal imaging-guided photothermal and anti-inflammatory combination cancer therapy. Biomater Sci. 2021;9(14):5025-5034. doi:10.1039/d1bm00576f(IF:6.843)
[11] Jiang D, Xu M, Pei Y, et al. Core-matched nanoassemblies for targeted co-delivery of chemotherapy and photosensitizer to treat drug-resistant cancer. Acta Biomater. 2019;88:406-421. doi:10.1016/j.actbio.2019.02.009(IF:6.638)
[12] Yu Y, Chen J, Liu S, Cheng D. ROS-responsive organosilica nanocarrier for the targeted delivery of metformin against cancer with the synergistic effect of hypoglycemia. J Mater Chem B. 2021;9(30):6044-6055. doi:10.1039/d1tb01143j(IF:6.331)
[13] Liu A, Wang H, Hou X, et al. Combinatory antitumor therapy by cascade targeting of a single drug. Acta Pharm Sin B. 2020;10(4):667-679. doi:10.1016/j.apsb.2019.08.011(IF:5.808)
[14] Wang X, Song H, Fang L, Wu T. EIF4A3-mediated circPRKCI expression promotes triple-negative breast cancer progression by regulating WBP2 and PI3K/AKT signaling pathway. Cell Death Discov. 2022;8(1):92. Published 2022 Mar 2. doi:10.1038/s41420-022-00892-y(IF:5.241)
[15] Huang Y, Zheng W, Ji C, et al. Circular RNA circRPPH1 promotes breast cancer progression via circRPPH1-miR-512-5p-STAT1 axis. Cell Death Discov. 2021;7(1):376. Published 2021 Dec 6. doi:10.1038/s41420-021-00771-y(IF:5.241)
[16] Chen J, Huang Y, Hu X, Bian X, Nian S. Gastrodin prevents homocysteine-induced human umbilical vein endothelial cells injury via PI3K/Akt/eNOS and Nrf2/ARE pathway. J Cell Mol Med. 2021;25(1):345-357. doi:10.1111/jcmm.16073(IF:4.486)
[17] Cheng X, Li D, Qi T, Sun J, Zhou T, Zheng WV. Objective to identify and verify the regulatory mechanism of DTNBP1 as a prognostic marker for hepatocellular carcinoma. Sci Rep. 2022;12(1):211. Published 2022 Jan 7. doi:10.1038/s41598-021-04055-4(IF:4.380)
[18] Tian R, Wang Z, Niu R, Wang H, Guan W, Chang J. Tumor Exosome Mimicking Nanoparticles for Tumor Combinatorial Chemo-Photothermal Therapy. Front Bioeng Biotechnol. 2020;8:1010. Published 2020 Aug 31. doi:10.3389/fbioe.2020.01010(IF:3.644)
[19] Wei B, Wang Z, Lian Q, Chi B, Ma S. hsa_circ_0139402 Promotes Bladder Cancer Progression by Regulating hsa-miR-326/PAX8 Signaling. Dis Markers. 2022;2022:9899548. Published 2022 Feb 1. doi:10.1155/2022/9899548(IF:3.434)
[20] Cui Z, Li Y, Liu G, Jiang Y. miR-103a-3p Silencing Ameliorates Calcium Oxalate Deposition in Rat Kidney by Activating the UMOD/TRPV5 Axis. Dis Markers. 2022;2022:2602717. Published 2022 Feb 23. doi:10.1155/2022/2602717(IF:3.434)
[21] Feng H, Tang J, Zhang P, Miao Y, Wu T, Cheng Z. Anti-adipogenic 18,19-seco-ursane stereoisomers and oleane-type saponins from Ilex cornuta leaves. Phytochemistry. 2020;175:112363. doi:10.1016/j.phytochem.2020.112363(IF:3.044)
[22] Zhu D, Chen C, Xia Y, Kong LY, Luo J. A Purified Resin Glycoside Fraction from Pharbitidis Semen Induces Paraptosis by Activating Chloride Intracellular Channel-1 in Human Colon Cancer Cells. Integr Cancer Ther. 2019;18:1534735418822120. doi:10.1177/1534735418822120(IF:2.634)
[23] Liu Y, Chen G, Wang B, Wu H, Zhang Y, Ye H. Silencing circRNA protein kinase C iota (circ-PRKCI) suppresses cell progression and glycolysis of human papillary thyroid cancer through circ-PRKCI/miR-335/E2F3 ceRNA axis. Endocr J. 2021;68(6):713-727. doi:10.1507/endocrj.EJ20-0726(IF:2.349)
[24] Zhu M, Wu Y, Wang Z, et al. miR-128-3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D. Oncol Lett. 2021;21(2):152. doi:10.3892/ol.2020.12413(IF:2.311)
[25] Chen L, Wu G, Li Y, Cai Q. Anesthetic propofol suppresses growth and metastasis of lung adenocarcinoma in vitro through downregulating circ-MEMO1-miR-485-3p-NEK4 ceRNA axis [published online ahead of print, 2022 May 6]. Histol Histopathol. 2022;18465. doi:10.14670/HH-18-465(IF:2.303)
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2-氨基-5-甲酰基噻唑-A2114

发表于由

Synthonix专注于鼓励药物化学家推动药物发现的界限。

自2003年以来，我们根据可旋转键，不寻常的键角，多种功能，代谢稳定性，分子量，水溶性，sp3杂交和三维结构等因素设计我们的产品在生物学上相关，使它们更适合蛋白质相互作用。结果是更有效的药物发现工具和更快的铅鉴定，药物化学家能够获得更具生物活性的化学空间。

 

2-氨基-5-甲酰基噻唑-A2114

产品信息

CAS1003-61-8

式C4H4N2OS

纯度96％

名称2-氨基-5-甲酰基噻唑-A2114

MDLMFCD02179570

MW128.15

inChi KeyHZKMBJCDAXLMDN-UHFFFAOYAQ

SKUA2114

 

 

 

货号

品名

规格

品牌

A2114G1 

2-AMINO-5-FORMYLTHIAZOLE – A2114

1g

synthonix

A2114G5 

2-AMINO-5-FORMYLTHIAZOLE – A2114

5g

synthonix

B1261MG500 

1-(2-BROMOETHYL)ADAMANTANE – B1261

500mg

synthonix

B1261G1 

1-(2-BROMOETHYL)ADAMANTANE – B1261

1g

synthonix

A12155G1 

1-ADAMANTANEMETHYLAMINE – A12155

1g

synthonix

A12155G5 

1-ADAMANTANEMETHYLAMINE – A12155

5g

synthonix

M9144G1 

METHYL 5-METHYLPIPERIDINE-3-CARBOXYLATE HYDROCHLORIDE – M9144

1g

synthonix

M9144G5 

METHYL 5-METHYLPIPERIDINE-3-CARBOXYLATE HYDROCHLORIDE – M9144

5g

synthonix

M9144G10 

METHYL 5-METHYLPIPERIDINE-3-CARBOXYLATE HYDROCHLORIDE – M9144

10g

synthonix

A2079G1 

A-AMINOTRICYCLO[3.3.1.13,7]DECANE-1-PROPANOIC ACID – A2079

1g

synthonix

A7304MG100 

2-AZA-TRICYCLO[3.3.1.13,7]DECANE-5-CARBOXYLIC ACID METHYL ESTER HYDROCHLORIDE – A7304

100mg

synthonix

A7304MG250 

2-AZA-TRICYCLO[3.3.1.13,7]DECANE-5-CARBOXYLIC ACID METHYL ESTER HYDROCHLORIDE – A7304

250mg

synthonix

A7304MG500 

2-AZA-TRICYCLO[3.3.1.13,7]DECANE-5-CARBOXYLIC ACID METHYL ESTER HYDROCHLORIDE – A7304

500mg

synthonix

A7304G1 

2-AZA-TRICYCLO[3.3.1.13,7]DECANE-5-CARBOXYLIC ACID METHYL ESTER HYDROCHLORIDE – A7304

1g

synthonix

X0764MG250 

4-OXOADAMANTANE-1-CARBOXYLIC ACID – X0764

250mg

synthonix

X0764MG500 

4-OXOADAMANTANE-1-CARBOXYLIC ACID – X0764

500mg

synthonix

T1314G1 

1-ADAMANTANEACETIC ACID – T1314

1g

synthonix

T1314G5 

1-ADAMANTANEACETIC ACID – T1314

5g

synthonix

T1314G25 

1-ADAMANTANEACETIC ACID – T1314

25g

synthonix

T1278G1 

1,3-ADAMANTANEDIOL – T1278

1g

synthonix

T1278G5 

1,3-ADAMANTANEDIOL – T1278

5g

synthonix

T1272MG250 

1-ADAMANTANEETHYLSULFONAMIDE – T1272

250mg

synthonix

T1272MG500 

1-ADAMANTANEETHYLSULFONAMIDE – T1272

500mg

synthonix

T1257G25 

1-ADAMANTANECARBOXYLIC ACID – T1257

25g

synthonix

T1257G50 

1-ADAMANTANECARBOXYLIC ACID – T1257

50g

synthonix

T1257G100 

1-ADAMANTANECARBOXYLIC ACID – T1257

100g

synthonix

T1257G250 

1-ADAMANTANECARBOXYLIC ACID – T1257

250g

synthonix

T1257G500 

1-ADAMANTANECARBOXYLIC ACID – T1257

500g

synthonix

T1253MG250 

4-METHOXYTRICYCLO[3.3.1.13,7]DEC-3-ENE-1-CARBOXYLIC ACID METHYL ESTER – T1253

250mg

synthonix

T1253MG500 

4-METHOXYTRICYCLO[3.3.1.13,7]DEC-3-ENE-1-CARBOXYLIC ACID METHYL ESTER – T1253

500mg

synthonix

M1273MG250 

3-METHOXY-1-HYDROXYMETHYLADAMANTANE – M1273

250mg

synthonix

M1273MG500 

3-METHOXY-1-HYDROXYMETHYLADAMANTANE – M1273

500mg

synthonix

H1256G1 

3-HYDROXYADAMANTANE-1-CARBOXYLIC ACID – H1256

1g

synthonix

H1256G5 

3-HYDROXYADAMANTANE-1-CARBOXYLIC ACID – H1256

5g

synthonix

H1256G1 

3-HYDROXYADAMANTANE-1-CARBOXYLIC ACID – H1256

1g

synthonix

H1256G5 

3-HYDROXYADAMANTANE-1-CARBOXYLIC ACID – H1256

5g

synthonix

H1255G1 

3-HYDROXY-1-HYDROXYMETHYLADMANTANE – H1255

1g

synthonix

H1255G5 

3-HYDROXY-1-HYDROXYMETHYLADMANTANE – H1255

5g

synthonix

H1249MG250 

4-HYDROXY-1-HYDROXYMETHYLADMANTANE – H1249

250mg

synthonix

H1249MG500 

4-HYDROXY-1-HYDROXYMETHYLADMANTANE – H1249

500mg

synthonix

F1274MG250 

3-FLUOROADAMANTANE-1-METHANOL – F1274

250mg

synthonix

F1274MG500 

3-FLUOROADAMANTANE-1-METHANOL – F1274

500mg

synthonix

D1248MG250 

4,4-DIFLUOROADAMANTANE-1-CARBOXYLIC ACID – D1248

250mg

synthonix

D1248MG500 

4,4-DIFLUOROADAMANTANE-1-CARBOXYLIC ACID – D1248

500mg

synthonix

B8345MG250 

TERT-BUTYL AZIRIDINE-1-CARBOXYLATE – B8345

250mg

synthonix

B8345G1 

TERT-BUTYL AZIRIDINE-1-CARBOXYLATE – B8345

1g

synthonix

B8345G5 

TERT-BUTYL AZIRIDINE-1-CARBOXYLATE – B8345

5g

synthonix

A2114G1 

2-AMINO-5-FORMYLTHIAZOLE – A2114

1g

synthonix

A2114G5 

2-AMINO-5-FORMYLTHIAZOLE – A2114

5g

synthonix

B0253G1 

6-BROMOPYRIDINE-2-CARBOXALDEHYDE – B0253

1g

synthonix

B0253G5 

6-BROMOPYRIDINE-2-CARBOXALDEHYDE – B0253

5g

synthonix

B0253G10 

6-BROMOPYRIDINE-2-CARBOXALDEHYDE – B0253

10g

synthonix

B0253G25 

6-BROMOPYRIDINE-2-CARBOXALDEHYDE – B0253

25g

synthonix

B0253G50 

6-BROMOPYRIDINE-2-CARBOXALDEHYDE – B0253

50g

synthonix

B0293G5 

TERT-BUTYL 4-FORMYL-1-PIPERIDINECARBOXYLATE – B0293

5g

synthonix

B0293G10 

TERT-BUTYL 4-FORMYL-1-PIPERIDINECARBOXYLATE – B0293

10g

synthonix

B0293G25 

TERT-BUTYL 4-FORMYL-1-PIPERIDINECARBOXYLATE – B0293

25g

synthonix

B0293G50 

TERT-BUTYL 4-FORMYL-1-PIPERIDINECARBOXYLATE – B0293

50g

synthonix

B0293G100 

TERT-BUTYL 4-FORMYL-1-PIPERIDINECARBOXYLATE – B0293

100g

synthonix

B0293G5 

TERT-BUTYL 4-FORMYL-1-PIPERIDINECARBOXYLATE – B0293

5g

synthonix

B0293G10 

TERT-BUTYL 4-FORMYL-1-PIPERIDINECARBOXYLATE – B0293

10g

synthonix

B0293G25 

TERT-BUTYL 4-FORMYL-1-PIPERIDINECARBOXYLATE – B0293

25g

synthonix

B0293G50 

TERT-BUTYL 4-FORMYL-1-PIPERIDINECARBOXYLATE – B0293

50g

synthonix

B0293G100 

TERT-BUTYL 4-FORMYL-1-PIPERIDINECARBOXYLATE – B0293

100g

synthonix

B0405G1 

5-BROMO-2-FLUOROPYRIDINE-3-CARBOXALDEHYDE – B0405

1g

synthonix

B0405G5 

5-BROMO-2-FLUOROPYRIDINE-3-CARBOXALDEHYDE – B0406

5g

synthonix

B0405G10 

5-BROMO-2-FLUOROPYRIDINE-3-CARBOXALDEHYDE – B0407

10g

synthonix

B0405G25 

5-BROMO-2-FLUOROPYRIDINE-3-CARBOXALDEHYDE – B0408

25g

synthonix

B0405G50 

5-BROMO-2-FLUOROPYRIDINE-3-CARBOXALDEHYDE – B0409

50g

synthonix

B0537MG250 

5-BROMO-3-NITROPYRIDINE-2-CARBALDEHYDE – B0537

250mg

synthonix

B0537MG500 

5-BROMO-3-NITROPYRIDINE-2-CARBALDEHYDE – B0537

500mg

synthonix

B0537G1 

5-BROMO-3-NITROPYRIDINE-2-CARBALDEHYDE – B0537

1g

synthonix

B0537G5 

5-BROMO-3-NITROPYRIDINE-2-CARBALDEHYDE – B0537

5g

synthonix

B0656G1 

TERT-BUTYL 6-FORMYL-3,4-DIHYDRO-1H-PYRROLO[1,2-A]PYRAZINE-2-CARBOXYLATE – B0656

1g

synthonix

B0656G5 

TERT-BUTYL 6-FORMYL-3,4-DIHYDRO-1H-PYRROLO[1,2-A]PYRAZINE-2-CARBOXYLATE – B0656

5g

synthonix

B0656G10 

TERT-BUTYL 6-FORMYL-3,4-DIHYDRO-1H-PYRROLO[1,2-A]PYRAZINE-2-CARBOXYLATE – B0656

10g

synthonix

B1995G1 

3-(BENZYLOXY)PROPANAL – B1995

1g

synthonix

B1995G5 

3-(BENZYLOXY)PROPANAL – B1995

5g

synthonix

B2058G1 

4-BROMO-3-FORMYLINDOLE – B2058

1g

synthonix

B2058G5 

4-BROMO-3-FORMYLINDOLE – B2058

5g

synthonix

B2058G25 

4-BROMO-3-FORMYLINDOLE – B2058

25g

synthonix

B2070MG500 

4-BROMO-2-FORMYLTHIAZOLE – B2070

500mg

synthonix

B2070G1 

4-BROMO-2-FORMYLTHIAZOLE – B2070

1g

synthonix

B2070G5 

4-BROMO-2-FORMYLTHIAZOLE – B2070

5g

synthonix

B2116G1 

1-BENZYL-1H-IMIDAZOLE-5-CARBOXALDEHYDE – B2116

1g

synthonix

B2116G5 

1-BENZYL-1H-IMIDAZOLE-5-CARBOXALDEHYDE – B2116

5g

synthonix

B2116G10 

1-BENZYL-1H-IMIDAZOLE-5-CARBOXALDEHYDE – B2116

10g

synthonix

B2118G1 

2-BROMO-4-FORMYLTHIAZOLE – B2118

1g

synthonix

B2118G5 

2-BROMO-4-FORMYLTHIAZOLE – B2118

5g

synthonix

B2118G25 

2-BROMO-4-FORMYLTHIAZOLE – B2118

25g

synthonix

B2118G50 

2-BROMO-4-FORMYLTHIAZOLE – B2118

50g

synthonix

B2119G1 

2-BROMO-5-FORMYLTHIAZOLE – B2119

1g

synthonix

B2119G5 

2-BROMO-5-FORMYLTHIAZOLE – B2120

5g

synthonix

B2119G25 

2-BROMO-5-FORMYLTHIAZOLE – B2121

25g

synthonix

B2929G1 

4-BROMO-2-FLUORO-3-FORMYLPYRIDINE – B2929

1g

synthonix

B2929G5 

4-BROMO-2-FLUORO-3-FORMYLPYRIDINE – B2929

5g

synthonix

B3012G1 

3-BROMOPYRIDINE-5-CARBOXALDEHYDE – B3012

1g

synthonix

B3012G5 

3-BROMOPYRIDINE-5-CARBOXALDEHYDE – B3012

5g

synthonix

B3012G25 

3-BROMOPYRIDINE-5-CARBOXALDEHYDE – B3012

25g

synthonix

B3012G100 

3-BROMOPYRIDINE-5-CARBOXALDEHYDE – B3012

100g

synthonix

B4066G1 

1-(2,2-DIETHOXYETHYL)-5-NITRO-1H-PYRROLE-2-CARBOXYLIC ACID ETHYL ESTER – B4066

1g

synthonix

B4066G5 

1-(2,2-DIETHOXYETHYL)-5-NITRO-1H-PYRROLE-2-CARBOXYLIC ACID ETHYL ESTER – B4066

5g

synthonix

B4889G1 

2-BROMO-1,1-DIMETHOXYPROPANE – B4889

1g

synthonix

B4889G5 

2-BROMO-1,1-DIMETHOXYPROPANE – B4889

5g

synthonix

B4889G25 

2-BROMO-1,1-DIMETHOXYPROPANE – B4889

25g

synthonix

C0338G1 

2-CHLOROPYRIMIDINE-5-CARBOXYALDEHYDE – C0338

1g

synthonix

C0338G5 

2-CHLOROPYRIMIDINE-5-CARBOXYALDEHYDE – C0338

5g

synthonix

C0338G10 

2-CHLOROPYRIMIDINE-5-CARBOXYALDEHYDE – C0338

10g

synthonix

C0408G1 

2-CHLOROISONICOTINALDEHYDE – C0408

1g

synthonix

C0408G5 

2-CHLOROISONICOTINALDEHYDE – C0408

5g

synthonix

C0408G10 

2-CHLOROISONICOTINALDEHYDE – C0408

10g

synthonix

C0408G25 

2-CHLOROISONICOTINALDEHYDE – C0408

25g

synthonix

C0408G50 

2-CHLOROISONICOTINALDEHYDE – C0408

50g

synthonix

C0446G1 

4-CHLORO-7H-PYRROLO[2,3-D]PYRIMIDINE-5-CARBALDEHYDE – C0446

1g

synthonix

C0446G5 

4-CHLORO-7H-PYRROLO[2,3-D]PYRIMIDINE-5-CARBALDEHYDE – C0446

5g

synthonix

C0446G10 

4-CHLORO-7H-PYRROLO[2,3-D]PYRIMIDINE-5-CARBALDEHYDE – C0446

10g

synthonix

C0446G25 

4-CHLORO-7H-PYRROLO[2,3-D]PYRIMIDINE-5-CARBALDEHYDE – C0446

25g

synthonix

C0446G50 

4-CHLORO-7H-PYRROLO[2,3-D]PYRIMIDINE-5-CARBALDEHYDE – C0446

50g

synthonix

C2204G1 

5-CHLORO-2-FORMYLPHENYLBORONIC ACID – C2204

1g

synthonix

C2204G5 

5-CHLORO-2-FORMYLPHENYLBORONIC ACID – C2204

5g

synthonix

C2204G25 

5-CHLORO-2-FORMYLPHENYLBORONIC ACID – C2204

25g

synthonix

C2225G1 

2-CHLORO-5-FORMYLPHENYLBORONIC ACID – C2225

1g

synthonix

C2225G5 

2-CHLORO-5-FORMYLPHENYLBORONIC ACID – C2225

5g

synthonix

C2225G25 

2-CHLORO-5-FORMYLPHENYLBORONIC ACID – C2225

25g

synthonix

C2225G100

2-CHLORO-5-FORMYLPHENYLBORONIC ACID – C2225

100g

synthonix

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1：强大的进口辐射能力，血清、抗体、耗材、大部分限制进口品等。

2：产品种类齐全，经营超过700多个品牌，基本涵盖所有生物实验试剂耗材。

3：提供加急服务，货品一般1-2周到货。

4：富有竞争力的价格优势，绝大部分价格有优势。

5：多年积累良好的信誉，大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校，ROCHE，阿斯利康、国药、fisher等药企。

6：我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等几十家国外公司代理。

7：我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发，欢迎合作。