FCER1a Proteing

发表于2024年3月1日由上海金畔生物科技有限公司

产品说明书

FAQ

COA

已发表文献

性能参数

分子别名（Synonyms）	FcERI;Fc-epsilon RI-alpha; FCER1A
表达区间及表达系统（Source）	Mouse Fc Epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala24-Gln204.[Accession \| P20489]
分子量大小（Molecular Weight）	The protein has a predicted MW of 47.7 kDa. Due to glycosylation, the protein migrates to 68-72 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

性能参数

分子别名（Synonyms）	FcERI;Fc-epsilon RI-alpha; FCER1A
表达区间及表达系统（Source）	Mouse Fc Epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala24-Gln204.[Accession \| P20489]
分子量大小（Molecular Weight）	The protein has a predicted MW of 47.7 kDa. Due to glycosylation, the protein migrates to 68-72 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组小鼠Fc-Epsilon RIα/FCER1a蛋白(hFc标签) Mouse Fc Epsilon RI alpha/FCER1a Proteing

暂无内容

重组人FcεRIα/FCER1a蛋白(hFc标签) Human Fc epsilon RI alpha/FCER1a Protein

发表于2024年3月1日由上海金畔生物科技有限公司

重组人FcεRIα/FCER1a蛋白(hFc标签) Human Fc epsilon RI alpha/FCER1a Protein

产品说明书

FAQ

COA

已发表文献

性能参数

表达区间及表达系统（Source）	Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Val26-Gln205.[Accession \| P12319-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by Tris-Bis PAGE and HPLC
活性（Activity）	ELISA Data: Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 10ng/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

Ver. CN20230625

性能参数

表达区间及表达系统（Source）	Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Val26-Gln205.[Accession \| P12319-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by Tris-Bis PAGE and HPLC
活性（Activity）	ELISA Data: Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 10ng/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

Ver. CN20230625

重组人FcεRIα/FCER1a蛋白(hFc标签) Human Fc epsilon RI alpha/FCER1a Protein

暂无内容

重组人FcεRIα/FCER1a蛋白(His标签) Human Fc epsilon RI alpha/FCER1a Protein

发表于2024年3月1日由上海金畔生物科技有限公司

重组人FcεRIα/FCER1a蛋白(His标签) Human Fc epsilon RI alpha/FCER1a Protein

产品说明书

FAQ

COA

已发表文献

性能参数

表达区间及表达系统（Source）	Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with His tag at the C-terminal. It contains Val26-Gln205.[uniprot \| P12319-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.
内毒素（Endotoxin）	Less than 1EU per ug by the LAL method.
纯度（Purity）	> 95% as determined by Tris-Bis PAGE and HPLC
活性（Activity）	ELISA Data: Immobilized Human Fc epsilon RI alpha, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Fc epsilon RI alpha Antibody , hFc Tag with the EC50 of 14.1ng/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

Ver. CN20230525

性能参数

表达区间及表达系统（Source）	Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with His tag at the C-terminal. It contains Val26-Gln205.[uniprot \| P12319-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.
内毒素（Endotoxin）	Less than 1EU per ug by the LAL method.
纯度（Purity）	> 95% as determined by Tris-Bis PAGE and HPLC
活性（Activity）	ELISA Data: Immobilized Human Fc epsilon RI alpha, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Fc epsilon RI alpha Antibody , hFc Tag with the EC50 of 14.1ng/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

Ver. CN20230525

重组人FcεRIα/FCER1a蛋白(His标签) Human Fc epsilon RI alpha/FCER1a Protein

暂无内容

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

发表于2024年3月1日由上海金畔生物科技有限公司

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

产品说明书

FAQ

COA

已发表文献

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation. The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities only recognizing IgG aggregates surrounding multivalent antigens. Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta. The only inhibitory member in human and mouse, Fc gamma RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses.

产品性质

别名（Synonyms）	CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI
Uniprot No.	P12314-1
表达区间及表达系统（Source）	Recombinant Human Fc gamma RI/CD64 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gln16-Pro288.
分子量(Molecular Weight)	The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
外观(Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC
活性（Activity）	SPR Data: Human Fc gamma RI, His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 11.3nM as determined in SPR assay.
内毒素（Endotoxin）	< 1.0 EU per 1μg of the protein by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

产品数据

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

HB220624

产品性质

别名（Synonyms）	CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI
Uniprot No.	P12314-1
表达区间及表达系统（Source）	Recombinant Human Fc gamma RI/CD64 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gln16-Pro288.
分子量(Molecular Weight)	The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
外观(Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC
活性（Activity）	SPR Data: Human Fc gamma RI, His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 11.3nM as determined in SPR assay.
内毒素（Endotoxin）	< 1.0 EU per 1μg of the protein by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

产品数据

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

HB220624

暂无内容

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重组小鼠Fc-Epsilon RIα/FCER1a蛋白(hFc标签) Mouse Fc Epsilon RI alpha/FCER1a Proteing

产品说明书

FAQ

COA

已发表文献

重组人FcεRIα/FCER1a蛋白(hFc标签) Human Fc epsilon RI alpha/FCER1a Protein

产品说明书

FAQ

COA

已发表文献

重组人FcεRIα/FCER1a蛋白(His标签) Human Fc epsilon RI alpha/FCER1a Protein

产品说明书

FAQ

COA

已发表文献

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

产品说明书

FAQ

COA

已发表文献