PPase无机焦磷酸酶 Inorganic Pyrophosphatase

PPase无机焦磷酸酶 Inorganic Pyrophosphatase

产品说明书

FAQ

COA

已发表文献

本品是大肠杆菌重组表达来源的无机焦磷酸酶,是一种催化一分子焦磷酸盐转化为两分子磷酸盐离子的酶。这是一个高放能的反应,因此该反应可偶联到一些热力学上不利的转化,以便驱动这些转化进行到完全。无机焦磷酸酶(PPase)催化无机焦磷酸盐水解生成正磷酸盐。在分子生物学中可用于反转录反应中提高RNA产量。

 

产品性质

来源(Source

重组E. coli

最适温度(Optimum Temperature

25℃

储存缓冲液(Storage Buffer

50 mM Tris-HCl pH 7.9100 mM NaCl10 mM DTT1 mM EDTA0.1% TritonX-10050%甘油

活性单位定义(Unit Definition

在标准反应条件下,每分钟催化水解PPI,产生1 μmol Pi所需要的酶量为1个活性单位。(标准反应为:500 μL体系中包含100 mM Tris-HCl pH7.22 mM MgCl22 mM Ppi25℃反应10 min

【注】:具体产品质检数据及更多指标请查看批次质检报告。

 

产品组分

组分编号

组分名称

产品编号/规格

10658ES10

(10 U)

10658ES60

(100 U)

10658ES80

1000 U

10658

Inorganic Pyrophosphatase 无机焦磷酸酶

10 μL

100 μL

1000 μL

运输和保存方法

干冰运输。-20℃保存,有效期一年。推荐分装保存,避免反复冻融。

 

注意事项

1、该产品在多种反应Buffer中均具有活性,通常本产品在HDA扩增以及LAMP扩增等实验中可直接接入。

2、该产品最适反应温度为25℃,其在16-37℃均有活性,65℃ 10 min可使该酶失活。

3、为了您的安全和健康,请穿实验服并戴一次性手套操作。

HB220701

PPase无机焦磷酸酶 Inorganic Pyrophosphatase

暂无内容

PPase无机焦磷酸酶 Inorganic Pyrophosphatase

暂无内容

本品是大肠杆菌重组表达来源的无机焦磷酸酶,是一种催化一分子焦磷酸盐转化为两分子磷酸盐离子的酶。这是一个高放能的反应,因此该反应可偶联到一些热力学上不利的转化,以便驱动这些转化进行到完全。无机焦磷酸酶(PPase)催化无机焦磷酸盐水解生成正磷酸盐。在分子生物学中可用于反转录反应中提高RNA产量。

 

产品性质

来源(Source

重组E. coli

最适温度(Optimum Temperature

25℃

储存缓冲液(Storage Buffer

50 mM Tris-HCl pH 7.9100 mM NaCl10 mM DTT1 mM EDTA0.1% TritonX-10050%甘油

活性单位定义(Unit Definition

在标准反应条件下,每分钟催化水解PPI,产生1 μmol Pi所需要的酶量为1个活性单位。(标准反应为:500 μL体系中包含100 mM Tris-HCl pH7.22 mM MgCl22 mM Ppi25℃反应10 min

【注】:具体产品质检数据及更多指标请查看批次质检报告。

 

产品组分

组分编号

组分名称

产品编号/规格

10658ES10

(10 U)

10658ES60

(100 U)

10658ES80

1000 U

10658

Inorganic Pyrophosphatase 无机焦磷酸酶

10 μL

100 μL

1000 μL

运输和保存方法

干冰运输。-20℃保存,有效期一年。推荐分装保存,避免反复冻融。

 

注意事项

1、该产品在多种反应Buffer中均具有活性,通常本产品在HDA扩增以及LAMP扩增等实验中可直接接入。

2、该产品最适反应温度为25℃,其在16-37℃均有活性,65℃ 10 min可使该酶失活。

3、为了您的安全和健康,请穿实验服并戴一次性手套操作。

HB220701

PPase无机焦磷酸酶 Inorganic Pyrophosphatase

暂无内容

PPase无机焦磷酸酶 Inorganic Pyrophosphatase

暂无内容

无机焦磷酸酶Pyrophosphatase GMP级(0.1 U/μL)

无机焦磷酸酶Pyrophosphatase GMP级(0.1 U/μL)

产品说明书

FAQ

COA

已发表文献

 

This product is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reaction.

This product is produced in accordance with GMP process requirements, and the product is provided in liquid form

 

Product Properties

Source

Recombinant E.coli with Pyrophosphatase gene from yeast

Optimum Temperature

25℃

Storage Buffer

50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% v/vTritonX-100, 50%v/vglycerin

Unit Definition

One unit (U) is the amount of enzyme needed to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.The standard reaction is: 500 μL system contains 100 mM Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min

 

Contents

Catalog No.

Name

Catalog No./Specification

10672ES03

(1 U)

10672ES10

(10 U)

10672ES60

(100 U)

10672

Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)

10 μL

100 μL

1 mL

 

Shipping and Storage

The Pyrophosphatase, Inorganic GMP-grade products shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

 

Notes

1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.

2.The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, 65℃ can inactivate the enzyme for 10 minutes.

3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this

 

HB220701

无机焦磷酸酶Pyrophosphatase GMP级(0.1 U/μL)

暂无内容

无机焦磷酸酶Pyrophosphatase GMP级(0.1 U/μL)

暂无内容

 

This product is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reaction.

This product is produced in accordance with GMP process requirements, and the product is provided in liquid form

 

Product Properties

Source

Recombinant E.coli with Pyrophosphatase gene from yeast

Optimum Temperature

25℃

Storage Buffer

50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% v/vTritonX-100, 50%v/vglycerin

Unit Definition

One unit (U) is the amount of enzyme needed to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.The standard reaction is: 500 μL system contains 100 mM Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min

 

Contents

Catalog No.

Name

Catalog No./Specification

10672ES03

(1 U)

10672ES10

(10 U)

10672ES60

(100 U)

10672

Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)

10 μL

100 μL

1 mL

 

Shipping and Storage

The Pyrophosphatase, Inorganic GMP-grade products shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

 

Notes

1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.

2.The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, 65℃ can inactivate the enzyme for 10 minutes.

3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this

 

HB220701

无机焦磷酸酶Pyrophosphatase GMP级(0.1 U/μL)

暂无内容

无机焦磷酸酶Pyrophosphatase GMP级(0.1 U/μL)

暂无内容

无机焦磷酸酶GMP级|Pyrophosphatase,Inorganic GMP-grade

无机焦磷酸酶GMP级|Pyrophosphatase,Inorganic GMP-grade

产品说明书

FAQ

COA

已发表文献

Pyrophosphatase, Inorganic GMP-grade (1 U/μL) is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reaction.

This product is produced in accordance with GMP process requirements, and the product is provided in liquid form

 

Product Properties

Source

Recombinant E.coli with   Pyrophosphatase gene from yeast

Optimum   Temperature

25℃

Storage   Buffer

50 mM   Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% v/vTritonX-100, 50%v/vglycerin

Unit   Definition

One unit (U) is the amount of enzyme needed   to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.The standard reaction is: 500 μL system contains 100 mM   Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min

 

Contents

Catalog No.

Name

Catalog No./Specification

 

10620ES10

(10 U)

10620ES60

(100 U)

10620ES80

(1,000   U)

10620ES99

(40 KU)

10620

Pyrophosphatase, Inorganic   GMP-grade (1 U/μL)

10 μL

100 μL

1 mL

40 mL

 

Shipping and Storage

The Pyrophosphatase, Inorganic GMP-grade products shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

 

Notes

1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.

2.The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, 65℃ can inactivate the enzyme for 10 minutes.

3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this. 

HB220701

无机焦磷酸酶GMP级|Pyrophosphatase,Inorganic GMP-grade

暂无内容

[1] Li Q, You J, Qiao T, Zhong DB, Yu X. Sodium chloride stimulates the biomass and astaxanthin production by Haematococcus pluvialis via a two-stage cultivation strategy. Bioresour Technol. 2022;344(Pt A):126214. doi:10.1016/j.biortech.2021.126214(IF:9.642)
[2] Ma Y, Cheng B, Li Y, et al. Protective Effect of Nanoselenium on Renal Oxidative Damage Induced by Mercury in Laying Hens. Biol Trace Elem Res. 2022;200(8):3785-3797. doi:10.1007/s12011-021-02956-z(IF:3.738)

Pyrophosphatase, Inorganic GMP-grade (1 U/μL) is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reaction.

This product is produced in accordance with GMP process requirements, and the product is provided in liquid form

 

Product Properties

Source

Recombinant E.coli with   Pyrophosphatase gene from yeast

Optimum   Temperature

25℃

Storage   Buffer

50 mM   Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% v/vTritonX-100, 50%v/vglycerin

Unit   Definition

One unit (U) is the amount of enzyme needed   to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.The standard reaction is: 500 μL system contains 100 mM   Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min

 

Contents

Catalog No.

Name

Catalog No./Specification

 

10620ES10

(10 U)

10620ES60

(100 U)

10620ES80

(1,000   U)

10620ES99

(40 KU)

10620

Pyrophosphatase, Inorganic   GMP-grade (1 U/μL)

10 μL

100 μL

1 mL

40 mL

 

Shipping and Storage

The Pyrophosphatase, Inorganic GMP-grade products shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

 

Notes

1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.

2.The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, 65℃ can inactivate the enzyme for 10 minutes.

3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this. 

HB220701

无机焦磷酸酶GMP级|Pyrophosphatase,Inorganic GMP-grade

暂无内容

[1] Li Q, You J, Qiao T, Zhong DB, Yu X. Sodium chloride stimulates the biomass and astaxanthin production by Haematococcus pluvialis via a two-stage cultivation strategy. Bioresour Technol. 2022;344(Pt A):126214. doi:10.1016/j.biortech.2021.126214(IF:9.642)
[2] Ma Y, Cheng B, Li Y, et al. Protective Effect of Nanoselenium on Renal Oxidative Damage Induced by Mercury in Laying Hens. Biol Trace Elem Res. 2022;200(8):3785-3797. doi:10.1007/s12011-021-02956-z(IF:3.738)