PhyNexus PTV 92-20-19说明书

发表于2024年4月17日由上海金畔生物科技有限公司

美国phynexus公司是美国的分子生物学试剂耗材供应商，上海金畔生物科技有限公司为您带来phynexus公司的产品和服务

PhyNexus PTV 92-20-19说明书

C18 Mass Spectrometry PhyTip Columns

PhyNexus开发了一种自动化工艺，使用C18色谱柱和C4色谱柱对蛋白质/多肽进行脱盐或“清理” ，以简化样品制备，进行质谱分析（一次多96个）。当使用手动移液管进行反相纯化时，吸气溶液中的背压会给操作者带来不便，并导致结果不一致。PhyTip色谱柱具有与自动化相辅相成的一致性，易用性和可扩展性，可实现清理。凭借蛋白质结合的高容量和多功能性，PhyNexus C18色谱柱是开发流体MALDI或LC-MS工作流程的适选择。

由于PhyTip移液器吸头柱的床尺寸和容量增加，C18色谱柱与其他色谱柱相比，极性和非极性分子的选择性提高。这种较大的床尺寸将使捕获的平衡推动到增加量的捕获产物。当比较一些的C18的容量时，C18 PhyTip色谱柱的结合量是每毫升树脂蛋白质量的两倍多，同时具有相当的回收率和范围。

C18色谱柱比C4色谱柱更具疏水性，可提供出色的灵敏度和肽的结合。中等90Å和300Å孔均可用于肽和蛋白质片段的差异选择性。在C4和C18树脂之间进行选择时，主要区别在于C4由于其较短的长度和更接近极性二氧化硅基板而比C18稍微更具极性。因此，对于更多极性分子，C4树脂的选择性增加。

这些柱结合了多种蛋白质和多肽大小。结合能力高，较小或更极性的多肽损失较少。在普通的质谱兼容溶剂中回收样品。此外，凝胶过滤柱可用于进行快速且*自动化的96次缓冲液脱盐或缓冲液交换。

使用我们专有的双流色谱 C18提示完整的抗体结合和高终浓度。C18 PhyTip色谱柱兼容大多数8,12和96通道自动化液体处理机器人，包括 Agilent Technologies， Beckman Coulter， Dynamic Devices， Perkin Elmer， Hamilton和 Tecan。

PhyNexus开发了一种自动化工艺，使用C18PhyTip®色谱柱对蛋白质/多肽进行脱盐或“清理”，用于质谱（MS）分析。PhyTip柱通过反相固相萃取（SPE）结合蛋白质/肽，其利用长碳链C18树脂基于其疏水性捕获和分离肽。

PhyTip色谱柱由C18树脂组成，通过两个薄的惰性玻璃料屏幕保留在移液器吸头上。根据下游分析，PhyTip色谱柱可以在5μL床中使用90Å或300Å孔隙树脂制成，并且可以处理高达200μL的溶液。PhyTip色谱柱通过双流色谱处理肽，这是一种由控制体积和流速的液体处理器执行的来回流动的系统过程。

因此，该方法可用于清除中小规模的肽样品（每次多96个），用于基质辅助激光解吸电离（MALDI）或电喷雾电离（ESI）质谱。自动化过程的一部分。

PhyNexus MEA个人净化系统的样品处理

1.样品制备成0.1％三氟y酸（TFA）浓度

2.用100μL100％乙腈（ACN）润湿4个循环

3.用100μL0.1％TFA平衡1个循环

4.用185μL样品捕获4个循环

5.用100μL0.1％TFA洗涤1个循环

6.用100μL70％ACN + 0.1％TFA洗脱4个循环

每个循环包括一次抽吸和一次排出，流速为1 mL / min。每个洗脱抽吸/排出步骤以4个增量进行，暂停5秒，而所有其他抽吸/排出步骤以8个增量进行，暂停30秒。该处理旨在模拟手动移液，同时补偿背压。密封洗脱板以防止挥发性洗脱缓冲液在处理期间蒸发，并且将溶液真空脱气以进一步确保空气不进入柱。

质谱PhyTip色谱柱可用于这些机器人系统。

树脂	机器人系统	提示量	树脂量	数量/箱	零件号	描述
C18300Å	Agilent Velocity 11 Bravo和V-Prep	200	20	96	PTV 92-20-19	每盒含有20μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	Beckman FX＆NX 96孔	200	5	96	PTB 92-05-19	每盒含有5μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	Beckman FX＆NX 96孔	200	20	96	PTB 92-20-19	每盒含有20μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	Caliper SciClone＆Zephyr 96 channel_now Perkin Elmer	200	5	96	PTZ 92-05-19	每盒含有5μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	Caliper SciClone＆Zephyr 96 channel_now Perkin Elmer	200	20	96	PTZ 92-20-19	每盒含有20μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	动态设备Oasis＆Lynx	200	5	96	PTD 92-05-19	每盒含有5μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	动态设备Oasis＆Lynx	1000	5	96	PTD 91-10-19	每盒含有10μLC18反相（300Å孔）树脂的96盒PhyTip柱（1 mL体积）
C18300Å	动态设备Oasis＆Lynx	1000	10	96	PTX 91-10-19	每盒含有10μLC18反相（300Å孔）树脂的96盒PhyTip柱（1 mL体积）
C18300Å	动态设备Oasis＆Lynx	200	20	96	PTD 92-20-19	每盒含有20μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	动态设备Oasis＆Lynx	1000	20	96	PTD 91-20-19	每盒含有20μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（1 mL体积）

PhyNexus PTV 92-20-02说明书

发表于2024年3月28日由上海金畔生物科技有限公司

美国phynexus公司是美国的分子生物学试剂耗材供应商，PhyNexus中国代理上海金畔生物科技有限公司为您带来phynexus公司的产品和服务

PhyNexus PTV 92-20-02说明书

Protein G PhyTip Columns

蛋白质G PhyTip柱

蛋白质G PhyTip柱显示功能性抗体（IgG）的高结合亲和力和高选择性亲和纯化。蛋白G柱显示出对不同同种型和IgG种类的高选择性，并获得高度浓缩的纯化蛋白

高结合亲和力
对不同物种的高选择性和抗体的同种型
IgG回收率为70-90％

使用我们专有的双流式色谱法提供的蛋白G提示可提供完整的抗体结合和高终浓度。PhyTip色谱柱兼容大多数8,12和96通道自动化液体处理机器人，包括 Agilent Technologies， Beckman Coulter， Dynamic Devices， Perkin Elmer， Hamilton和 Tecan。

含有蛋白G的PhyTip色谱柱

含有蛋白G的PhyTip色谱柱已经过优化，可用于特定的PhyNexus试剂和仪器流速/体积，如下所示。使用PhyTip ME 1000和ME 200纯化系统收集该信息

所有带有蛋白G的PhyTip色谱柱都配有推荐的PhyTip缓冲液，包括：捕获缓冲液 – 适用于需要添加额外缓冲液以补充样品体积并确保正确捕获pH值的情况。洗涤缓冲液I – 磷酸盐缓冲溶液pH 7.4洗涤缓冲液II – 盐水溶液。注意：没有缓冲能力以确保有效洗脱富集缓冲液 – 用于终洗脱步骤 – 磷酸盐缓冲溶液pH 2.5中和缓冲液。 – Tris缓冲溶液pH 9.0

注意：富集缓冲液以4 mL pH 2.5磷酸盐缓冲溶液的形式提供，如果要纯化的蛋白质需要较少的酸性洗脱条件，例如pH 2.8，则可以如下更改富集缓冲液的pH值：取1 mL标准富集缓冲液（pH值） 2.5）加入30μL1MTris Buffer标准中和缓冲液，得到1 mL pH 2.8洗脱缓冲液（实际pH值可能因体积精度而异）对于pH 3.0浓缩缓冲液，取1 mL标准富集缓冲液（pH 2.5））加入40μL1MTris Buffer标准中和缓冲液，得到1 mL pH 3.0洗脱缓冲液（实际pH值可能因体积精度而异）

对于中和步骤，添加25％v / v的洗脱体积，例如，如果洗脱体积为20μL，则添加5μL的1M Tris中和缓冲液

含有蛋白G树脂的200多个PhyTip色谱柱：

对于含有1mg BSA的5μgIgG2a（抗FITC MAb。）的200μL样品，使用下面显示的条件处理，在终样品体积中回收大于40％的原始IgG质量。此外，通过用考马斯检测的SDS-PAGE测定，回收的IgG纯度超过95％。

捕获：通过树脂床以250μL/分钟的流速通过树脂床捕获200μL样品。

纯化：200μLPhyNexusProtein G洗涤缓冲液I，以500μL/ min的流速通过树脂床两个循环，然后用洗涤缓冲液II进行第二次洗涤，在树脂床上通过两个循环流动速率为500μL/ min。

富集：用10μLPhyNexusProtein G Enrichment Buffer将蛋白质洗脱到溶液中，以500μL/ min的流速通过树脂床5个循环。用3μLPhyNexus蛋白G中和缓冲液中和。

出厂时含有的蛋白G富集缓冲液含有：111mM NaH2PO4,140mM NaCl，14.8mM H3PO4，pH3.0

含有蛋白G树脂的1000多种PhyTip色谱柱：

对于含有5μgBSA的10μgIgG2a（抗FITC MAb。）的500μL样品，使用下面显示的条件处理，在终样品体积中回收大于40％的原始IgG质量。此外，通过用考马斯检测的SDS-PAGE测定，回收的IgG纯度超过95％。

捕获：通过树脂床以250μL/分钟的流速通过四个循环捕获500μL样品。

纯化：1000μLPhyNexusProtein G洗涤缓冲液I，以500μL/ min的流速通过树脂床两个循环，然后用洗涤缓冲液II进行第二次洗涤，以500的流速通过树脂床两个循环。 μL/分钟。

富集：用15mL PhyNexus Protein G Enrichment Buffer将蛋白质洗脱到溶液中，以500μL/ min的流速通过树脂床5个循环。用5μLPhyNexus蛋白G中和缓冲液中和。

PhyTip色谱柱是PhyNexus*的捕获，纯化和富集TM工具，专为微量蛋白质样品制备而设计。PhyTip色谱柱适用于各种液体处理平台，并含有特定的亲和树脂，可满足特定应用要求。

PhyNexus建议使用含有IgG的PhyTip入门试剂盒作为标准，以验证所有应用中PhyTip色谱柱的使用。

用于纯化和富集的样品必须清澈且无颗粒物质。在与PhyTip色谱柱一起使用之前，强烈建议离心样品并仅使用澄清的上清液。

运输和存储

每包PhyTip色谱柱均按照高标准生产和质量控制，并在保留盒中运输，以保持每个PhyTip色谱柱中特定亲和树脂的完整性。本产品在环境温度下运输，但收到后应存放在4到8℃的标准实验室冰箱中。

不要冷冻或储存冷冻。
不使用时，请将盒盖关闭并密封，存放在冰箱中。
在干燥环境中长时间储存，不要让亲和树脂干燥。

含有蛋白G的PhyTip色谱柱在从PhyNexus发货时储存在甘油中。

Protein G PhyTip色谱柱可用于这些机器人系统。

机器人系统

Agilent Velocity 11 Bravo和V-Prep

Beckman FX＆NX 96孔

Caliper SciClone和Zephyr 96通道（现为Perkin Elmer）

动态设备Oasis＆Lynx

Hamilton STAR和Nimbus

Perkin Elmer Janus Varispan 4或8孔

Perkin Elmer Packard MiniTrack和Janus MDT 96频道

PhyNexus MEA或ME

Tecan Freedom Evo LiHa＆Tecan Te-MO

Tecan Freedom Evo MCA 96孔

树脂	机器人系统	提示量	树脂量	数量/箱	零件号	描述
蛋白质G.	Agilent Velocity 11 Bravo和V-Prep	200	20	96	PTV 92-20-02	一箱96个PhyTip柱（200μL体积）每柱20μL蛋白G亲和树脂
蛋白质G.	Beckman FX＆NX 96孔	200	五	96	PTB 92-05-02	一箱96个PhyTip柱（200μL体积）每柱5μL蛋白G亲和树脂
蛋白质G.	Beckman FX＆NX 96孔	200	20	96	PTB 92-20-02	一箱96个PhyTip柱（200μL体积）每柱20μL蛋白G亲和树脂
蛋白质G.	Caliper SciClone＆Zephyr 96 channel_now Perkin Elmer	200	五	96	PTZ 92-05-02	一箱96个PhyTip柱（200μL体积）每柱5μL蛋白G亲和树脂
蛋白质G.	Caliper SciClone＆Zephyr 96 channel_now Perkin Elmer	200	20	96	PTZ 92-20-02	一箱96个PhyTip柱（200μL体积）每柱20μL蛋白G亲和树脂
蛋白质G.	动态设备Lynx	1000	10	96	PTX 91-10-02	每盒含有10μL蛋白G亲和树脂的96个PhyTip柱（1 mL体积）
蛋白质G.	动态设备Lynx	1000	160	96	PTX 91-16-02	每盒含有160μL蛋白G亲和树脂的96个PhyTip柱（1 mL体积）
蛋白质G.	动态设备Lynx	1000	20	96	PTX 91-20-02	每盒含有20μL蛋白G亲和树脂的96个PhyTip柱（1 mL体积）
蛋白质G.	动态设备Lynx	1000	40	96	PTX 91-40-02	每盒含有40μL蛋白G亲和树脂的96个PhyTip柱（1 mL体积）
蛋白质G.	动态设备Lynx	1000	80	96	PTX 91-80-02	每盒含有80μL蛋白G亲和树脂的96个PhyTip柱（1 mL体积）

货号	品名
DAB 12-000-02	PhyTip column transfer tool for Beckman instrument
DAB 96-000-01	Adaptor for Lysate Direct PhyTip columns for Beckman
DAT 12-000-02	PhyTip column transfer tool for Tecan instrument
DAT 96-000-01	Adaptor for Lysate Direct PhyTip columns for Tecan
DPA 00-01-00	MEA Lysate Direct PhyTip column Adaptor kit (US)
DPA 00-01-01	MEA Lysate Direct PhyTip column Adaptor kit (UK)
DPA 00-01-02	MEA Lysate Direct PhyTip column Adaptor kit (European)
DPB 92-80-70	Lysate Direct PhyTip columns for plasmid DNA 1 box kit Beckman
DPB 92-80-70-10	Lysate Direct PhyTip columns for plasmid DNA 10 box kit Beckman
DPB 99-125-01	Precipitation Buffer for plasmid miniprep, 125 mL
DPR 11-80-70	Demo Kit, Lysate Direct PhyTip columns for plasmid DNA box of 12 Rainin
DPR 91-80-70	Lysate Direct PhyTip columns for plasmid DNA 1 box kit Rainin
DPR 91-80-70-10	Lysate Direct PhyTip columns for plasmid DNA 10 box kit Rainin
DPT 92-80-70	Lysate Direct PhyTip columns for plasmid DNA 1 box kit Tecan
DPT 92-80-70-10	Lysate Direct PhyTip columns for plasmid DNA 10 box kit Tecan
PCP 96-00-10	Box of 100 Elution Sample Plates: 96 x 200 μL v-bottom wells. Polypropylene
PCP 96-10-20	PhyTip Cover for Elution Plates: 100 covers per roll
PCP 96-20-10	Box of 5 Deep-well Sample Plates: 96 x 2 mL v-bottom wells. Polypropylene
PCP 96-60-00	Pack of Neutralization Reservoirs: 36 rows of 12 wells
PCS 00-000-01	Pipette Calibration Service
PDR 41-20-06	Box of 48 Desalting columns (1000 μL volume) containing 200 μL of 5K desalting resin per column.
PDR 41-60-06	Box of 48 Desalting columns (1000 μL volume) containing 600 μL of 5K desalting resin per column.
PDR 91-20-06	Box of 96 Desalting columns (1000 μL volume) containing 200 μL of 5K desalting resin per column.
PDR 91-60-06	Box of 96 Desalting columns (1000 μL volume) containing 600 μL of 5K desalting resin per column.
PHH 10-00-96	MEA Heater platform – single position, controllable temperature plate
PHR 00-000-00	MEA Personal Purification System
PHR 00-000-01	MEA Personal Purification System – UK Voltage version
PHR 00-000-02	MEA Personal Purification System – European Voltage version
PHR 00-000-03	MEA Personal Purification System – “Other” Voltage version
PHR 96-000-00	MEA Personal Purification System – US Voltage version
PHR 96-000-01	MEA Personal Purification System – UK Voltage version
PHR 96-000-02	MEA Personal Purification System – European Voltage version
PHR 96-000-03	MEA Personal Purification System – “Other” Voltage version
PHR 96-020-00	200+ pipette head for MEA System
PHR 96-100-00	1000+ pipette head for MEA System
PHR 96-900-01	Laptop computer for MEA Platform
PHW 96-010-01	U.S. Service Agreement
PHW 96-010-02	U.S. Service Agreement with Loaner System
PHW 96-010-03	European Service Agreement
PHW 96-010-04	European Service Agreement with Loaner System
PMT 92-05-01	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein A affinity resin per column
PMT 92-05-02	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein G affinity resin per column
PMT 92-05-03	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of IMAC affinity resin per column
PMT 92-05-04	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Glutathione affinity resin per column
PMT 92-05-05	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Streptavidin per column
PMT 92-05-07	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of ProPlus affinity resin per column
PMT 92-05-09	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Normal Phase resin per column
PMT 92-05-11	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Anion resin per column
PMT 92-05-12	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Anion resin per column
PMT 92-05-13	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Cation resin per column
PMT 92-05-14	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Cation resin per column
PMT 92-05-21	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect LC-kappa (Hu) resin per column
PMT 92-05-22	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect LC-lambda (Hu) resin per column
PMT 92-05-23	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgC-Fc (Hu) resin per column
PMT 92-05-24	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgC-Fc (ms) resin per column
PMT 92-05-25	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgM resin per column
PMT 92-05-NS	Box of 96 PhyScreen Ni-IMAC Resin Screening Toolkit containing 5ul of 12 Ni-IMAC resins
PMT 92-20-01	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein A affinity resin per column
PMT 92-20-02	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein G affinity resin per column
PMT 92-20-03	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of IMAC affinity resin per column
PMT 92-20-04	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Glutathione affinity resin per column
PMT 92-20-05	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Streptavidin per column
PMT 92-20-07	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of ProPlus affinity resin per column
PMT 92-20-09	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Normal Phase resin per column
PMT 92-20-11	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Anion resin per column
PMT 92-20-12	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Anion resin per column
PMT 92-20-13	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Cation resin per column
PMT 92-20-14	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Cation resin per column
PMT 92-20-21	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect LC-kappa (Hu) resin per column
PMT 92-20-22	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect LC-lambda (Hu) resin per column
PMT 92-20-23	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgC-Fc (Hu) resin per column
PMT 92-20-24	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgC-Fc (ms) resin per column
PMT 92-20-25	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgM resin per column
PSA 10-00-00	USB to Serial Adaptor
PTB 92-05-01	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein A affinity resin per column
PTB 92-05-02	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein G affinity resin per column
PTB 92-05-03	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of IMAC affinity resin per column
PTB 92-05-04	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Glutathione affinity resin per column
PTB 92-05-05	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Streptavidin per column
PTB 92-05-07	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of ProPlus affinity resin per column
PTB 92-05-09	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Normal Phase resin per column
PTB 92-05-11	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Anion resin per column
PTB 92-05-12	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Anion resin per column
PTB 92-05-13	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Cation resin per column
PTB 92-05-14	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Cation resin per column
PTB 92-05-21	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect LC-kappa (Hu) resin per column
PTB 92-05-22	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect LC-lambda (Hu) resin per column
PTB 92-05-23	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTB 92-05-24	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgC-Fc (ms) resin per column
PTB 92-05-25	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgM resin per column
PTB 92-05-NS	Box of 96 PhyScreen Ni-IMAC Resin Screening Toolkit containing 5 μL of 12 Ni-IMAC resins
PTB 92-20-01	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein A affinity resin per column
PTB 92-20-02	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein G affinity resin per column
PTB 92-20-03	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of IMAC affinity resin per column
PTB 92-20-04	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Glutathione affinity resin per column
PTB 92-20-05	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Streptavidin per column
PTB 92-20-07	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of ProPlus affinity resin per column
PTB 92-20-09	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Normal Phase resin per column
PTB 92-20-11	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Anion resin per column
PTB 92-20-12	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Anion resin per column
PTB 92-20-13	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Cation resin per column
PTB 92-20-14	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Cation resin per column
PTB 92-20-21	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect LC-kappa (Hu) resin per column
PTB 92-20-22	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect LC-lambda (Hu) resin per column
PTB 92-20-23	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTB 92-20-24	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgC-Fc (ms) resin per column
PTB 92-20-25	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgM resin per column
PTD 22-05-NS	Box of 24 PhyScreen Ni-IMAC Resin Screening Toolkit containing 5 μl of 12 Ni-IMAC resins
PTD 41-10-01	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Protein A affinity resin per column
PTD 41-10-02	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Protein G affinity resin per column
PTD 41-10-03	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of IMAC affinity resin per column
PTD 41-10-04	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Glutathione affinity resin per column
PTD 41-10-05	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Streptavidin affinity resin per column
PTD 41-10-07	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of ProPlus affinity resin per column
PTD 41-10-09	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Normal Phase affinity resin per column
PTD 41-16-01	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Protein A affinity resin per column
PTD 41-16-02	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Protein G affinity resin per column
PTD 41-16-03	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of IMAC affinity resin per column
PTD 41-16-04	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Glutathione affinity resin per column
PTD 41-16-05	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Streptavidin affinity resin per column
PTD 41-16-07	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of ProPlus affinity resin per column
PTD 41-20-01	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Protein A affinity resin per column
PTD 41-20-02	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Protein G affinity resin per column
PTD 41-20-03	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of IMAC affinity resin per column
PTD 41-20-04	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Glutathione affinity resin per column
PTD 41-20-05	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Streptavidin affinity resin per column
PTD 41-20-07	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of ProPlus affinity resin per column
PTD 41-20-09	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Normal Phase affinity resin per column
PTD 41-32-01	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Protein A affinity resin per column
PTD 41-32-02	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Protein G affinity resin per column
PTD 41-32-03	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of IMAC affinity resin per column
PTD 41-32-04	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Glutathione affinity resin per column
PTD 41-32-05	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Streptavidin affinity resin per column
PTD 41-32-07	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of ProPlus affinity resin per column
PTD 41-40-01	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Protein A affinity resin per column
PTD 41-40-02	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Protein G affinity resin per column
PTD 41-40-03	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of IMAC affinity resin per column
PTD 41-40-04	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Glutathione affinity resin per column
PTD 41-40-05	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Streptavidin affinity resin per column
PTD 41-40-07	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of ProPlus affinity resin per column
PTD 41-80-01	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Protein A affinity resin per column
PTD 41-80-02	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Protein G affinity resin per column
PTD 41-80-03	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of IMAC affinity resin per column
PTD 41-80-04	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Glutathione affinity resin per column
PTD 41-80-05	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Streptavidin affinity resin per column
PTD 41-80-07	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of ProPlus affinity resin per column
PTD 91-10-01	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Protein A affinity resin per column
PTD 91-10-02	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Protein G affinity resin per column
PTD 91-10-03	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of IMAC affinity resin per column
PTD 91-10-04	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Glutathione affinity resin per column
PTD 91-10-05	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Streptavidin affinity resin per column
PTD 91-10-07	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of ProPlus affinity resin per column
PTD 91-10-09	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Normal Phase affinity resin per column
PTD 91-16-01	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Protein A affinity resin per column
PTD 91-16-02	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Protein G affinity resin per column
PTD 91-16-03	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of IMAC affinity resin per column
PTD 91-16-04	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Glutathione affinity resin per column
PTD 91-16-05	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Streptavidin affinity resin per column
PTD 91-16-07	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of ProPlus affinity resin per column
PTD 91-20-01	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Protein A affinity resin per column
PTD 91-20-02	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Protein G affinity resin per column
PTD 91-20-03	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of IMAC affinity resin per column
PTD 91-20-04	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Glutathione affinity resin per column
PTD 91-20-05	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Streptavidin affinity resin per column
PTD 91-20-07	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of ProPlus affinity resin per column
PTD 91-20-09	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Normal Phase affinity resin per column
PTD 91-32-01	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Protein A affinity resin per column
PTD 91-32-02	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Protein G affinity resin per column
PTD 91-32-03	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of IMAC affinity resin per column
PTD 91-32-04	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Glutathione affinity resin per column
PTD 91-32-05	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Streptavidin affinity resin per column
PTD 91-32-07	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of ProPlus affinity resin per column
PTD 91-40-01	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Protein A affinity resin per column
PTD 91-40-02	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Protein G affinity resin per column
PTD 91-40-03	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of IMAC affinity resin per column
PTD 91-40-04	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Glutathione affinity resin per column
PTD 91-40-05	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Streptavidin affinity resin per column
PTD 91-40-07	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of ProPlus affinity resin per column
PTD 91-80-01	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Protein A affinity resin per column
PTD 91-80-02	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Protein G affinity resin per column
PTD 91-80-03	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of IMAC affinity resin per column
PTD 91-80-04	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Glutathione affinity resin per column
PTD 91-80-05	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Streptavidin affinity resin per column
PTD 91-80-07	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of ProPlus affinity resin per column
PTD 92-05-01	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein A affinity resin per column
PTD 92-05-02	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein G affinity resin per column
PTD 92-05-03	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of IMAC affinity resin per column
PTD 92-05-04	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Glutathione affinity resin per column
PTD 92-05-05	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Streptavidin affinity resin per column
PTD 92-05-07	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of ProPlus affinity resin per column
PTD 92-05-09	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Normal Phase 1 affinity resin per column
PTD 92-05-NS	Box of 96 PhyScreen Ni-IMAC Resin Screening Toolkit containing 5 μl of 12 Ni-IMAC resins
PTD 92-20-01	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Protein A affinity resin per column
PTD 92-20-02	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Protein G affinity resin per column
PTD 92-20-03	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of IMAC affinity resin per column
PTD 92-20-04	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Glutathione affinity resin per column
PTD 92-20-05	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Streptavidin affinity resin per column
PTD 92-20-07	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of ProPlus affinity resin per column
PTD 92-20-09	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Normal Phase 1 affinity resin per column
PTH 92-05-01	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein A affinity resin per column
PTH 92-05-02	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein G affinity resin per column
PTH 92-05-03	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of IMAC affinity resin per column
PTH 92-05-04	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Glutathione affinity resin per column
PTH 92-05-05	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Streptavidin per column
PTH 92-05-07	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of ProPlus affinity resin per column
PTH 92-05-09	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Normal Phase resin per column
PTH 92-05-11	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Anion resin per column
PTH 92-05-12	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Anion resin per column
PTH 92-05-13	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Cation resin per column
PTH 92-05-14	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Cation resin per column
PTH 92-05-NS	Box of 96 PhyScreen Ni-IMAC Resin Screening Toolkit containing 5ul of 12 Ni-IMAC resins
PTH 92-20-01	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein A affinity resin per column
PTH 92-20-02	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein G affinity resin per column
PTH 92-20-03	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of IMAC affinity resin per column
PTH 92-20-04	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Glutathione affinity resin per column
PTH 92-20-05	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Streptavidin per column
PTH 92-20-07	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of ProPlus affinity resin per column
PTH 92-20-09	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Normal Phase resin per column
PTH 92-20-11	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Anion resin per column
PTH 92-20-12	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Anion resin per column
PTH 92-20-13	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Cation resin per column
PTH 92-20-14	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Cation resin per column
PTH 93-05-21	Box of 96 PhyTip columns (300 μL volume) containing 5 μL of CaptureSelect LC-kappa (Hu) resin per column
PTH 93-05-22	Box of 96 PhyTip columns (300 μL volume) containing 5 μL of CaptureSelect LC-lambda (Hu) resin per column
PTH 93-05-23	Box of 96 PhyTip columns (300 μL volume) containing 5 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTH 93-05-24	Box of 96 PhyTip columns (300 μL volume) containing 5 μL of CaptureSelect IgC-Fc (ms) resin per column
PTH 93-05-25	Box of 96 PhyTip columns (300 μL volume) containing 5 μL of CaptureSelect IgM resin per column
PTH 93-20-21	Box of 96 PhyTip columns (300 μL volume) containing 20 μL of CaptureSelect LC-kappa (Hu) resin per column
PTH 93-20-22	Box of 96 PhyTip columns (300 μL volume) containing 20 μL of CaptureSelect LC-lambda (Hu) resin per column
PTH 93-20-23	Box of 96 PhyTip columns (300 μL volume) containing 20 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTH 93-20-24	Box of 96 PhyTip columns (300 μL volume) containing 20 μL of CaptureSelect IgC-Fc (ms) resin per column
PTH 93-20-25	Box of 96 PhyTip columns (300 μL volume) containing 20 μL of CaptureSelect IgM resin per column
PTJ 41-10-01	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Protein A affinity resin per column
PTJ 41-10-02	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Protein G affinity resin per column
PTJ 41-10-03	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of IMAC affinity resin per column
PTJ 41-10-04	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Glutathione affinity resin per column
PTJ 41-10-05	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Streptavidin affinity resin per column
PTJ 41-10-07	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of ProPlus affinity resin per column
PTJ 41-10-09	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Normal Phase affinity resin per column
PTJ 41-16-01	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Protein A affinity resin per column
PTJ 41-16-02	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Protein G affinity resin per column
PTJ 41-16-03	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of IMAC affinity resin per column
PTJ 41-16-04	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Glutathione affinity resin per column
PTJ 41-16-05	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Streptavidin affinity resin per column
PTJ 41-16-07	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of ProPlus affinity resin per column
PTJ 41-20-01	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Protein A affinity resin per column
PTJ 41-20-02	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Protein G affinity resin per column
PTJ 41-20-03	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of IMAC affinity resin per column
PTJ 41-20-04	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Glutathione affinity resin per column
PTJ 41-20-05	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Streptavidin affinity resin per column
PTJ 41-20-07	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of ProPlus affinity resin per column
PTJ 41-20-09	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Normal Phase affinity resin per column
PTJ 41-32-01	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Protein A affinity resin per column
PTJ 41-32-02	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Protein G affinity resin per column
PTJ 41-32-03	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of IMAC affinity resin per column
PTJ 41-32-04	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Glutathione affinity resin per column
PTJ 41-32-05	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Streptavidin affinity resin per column
PTJ 41-32-07	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of ProPlus affinity resin per column
PTJ 41-40-01	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Protein A affinity resin per column
PTJ 41-40-02	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Protein G affinity resin per column
PTJ 41-40-03	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of IMAC affinity resin per column
PTJ 41-40-04	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Glutathione affinity resin per column
PTJ 41-40-05	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Streptavidin affinity resin per column
PTJ 41-40-07	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of ProPlus affinity resin per column
PTJ 41-80-01	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Protein A affinity resin per column
PTJ 41-80-02	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Protein G affinity resin per column
PTJ 41-80-03	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of IMAC affinity resin per column
PTJ 41-80-04	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Glutathione affinity resin per column
PTJ 41-80-05	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Streptavidin affinity resin per column
PTJ 41-80-07	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of ProPlus affinity resin per column
PTJ 91-10-01	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Protein A affinity resin per column
PTJ 91-10-02	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Protein G affinity resin per column
PTJ 91-10-03	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of IMAC affinity resin per column
PTJ 91-10-04	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Glutathione affinity resin per column
PTJ 91-10-05	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Streptavidin affinity resin per column
PTJ 91-10-07	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of ProPlus affinity resin per column
PTJ 91-10-09	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Normal Phase affinity resin per column
PTJ 91-16-01	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Protein A affinity resin per column
PTJ 91-16-02	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Protein G affinity resin per column
PTJ 91-16-03	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of IMAC affinity resin per column
PTJ 91-16-04	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Glutathione affinity resin per column
PTJ 91-16-05	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Streptavidin affinity resin per column
PTJ 91-16-07	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of ProPlus affinity resin per column
PTJ 91-20-01	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Protein A affinity resin per column
PTJ 91-20-02	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Protein G affinity resin per column
PTJ 91-20-03	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of IMAC affinity resin per column
PTJ 91-20-04	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Glutathione affinity resin per column
PTJ 91-20-05	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Streptavidin affinity resin per column
PTJ 91-20-07	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of ProPlus affinity resin per column
PTJ 91-20-09	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Normal Phase affinity resin per column
PTJ 91-32-01	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Protein A affinity resin per column
PTJ 91-32-02	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Protein G affinity resin per column
PTJ 91-32-03	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of IMAC affinity resin per column
PTJ 91-32-04	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Glutathione affinity resin per column
PTJ 91-32-05	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Streptavidin affinity resin per column
PTJ 91-32-07	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of ProPlus affinity resin per column
PTJ 91-40-01	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Protein A affinity resin per column
PTJ 91-40-02	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Protein G affinity resin per column
PTJ 91-40-03	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of IMAC affinity resin per column
PTJ 91-40-04	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Glutathione affinity resin per column
PTJ 91-40-05	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Streptavidin affinity resin per column
PTJ 91-40-07	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of ProPlus affinity resin per column
PTJ 91-80-01	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Protein A affinity resin per column
PTJ 91-80-02	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Protein G affinity resin per column
PTJ 91-80-03	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of IMAC affinity resin per column
PTJ 91-80-04	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Glutathione affinity resin per column
PTJ 91-80-05	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Streptavidin affinity resin per column
PTJ 91-80-07	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of ProPlus affinity resin per column
PTP 92-05-01	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein A affinity resin per column
PTP 92-05-02	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein G affinity resin per column
PTP 92-05-03	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of IMAC affinity resin per column
PTP 92-05-04	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Glutathione affinity resin per column
PTP 92-05-05	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Streptavidin per column
PTP 92-05-07	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of ProPlus affinity resin per column
PTP 92-05-09	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Normal Phase resin per column
PTP 92-05-11	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Anion resin per column
PTP 92-05-12	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Anion resin per column
PTP 92-05-13	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Cation resin per column
PTP 92-05-14	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Cation resin per column
PTP 92-05-21	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect LC-kappa (Hu) resin per column
PTP 92-05-22	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect LC-lambda (Hu) resin per column
PTP 92-05-23	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTP 92-05-24	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgC-Fc (ms) resin per column
PTP 92-05-25	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgM resin per column
PTP 92-05-NS	Box of 96 PhyScreen Ni-IMAC Resin Screening Toolkit containing 5ul of 12 Ni-IMAC resins
PTP 92-20-01	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein A affinity resin per column
PTP 92-20-02	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein G affinity resin per column
PTP 92-20-03	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of IMAC affinity resin per column
PTP 92-20-04	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Glutathione affinity resin per column
PTP 92-20-05	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Streptavidin per column
PTP 92-20-07	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of ProPlus affinity resin per column
PTP 92-20-09	Box of 96 PhyTip columns( 200 μL volume) containing 20 μL of Normal Phase resin per column
PTP 92-20-11	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Anion resin per column
PTP 92-20-12	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Anion resin per column
PTP 92-20-13	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Cation resin per column
PTP 92-20-14	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Cation resin per column
PTP 92-20-21	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect LC-kappa (Hu) resin per column
PTP 92-20-22	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect LC-lambda (Hu) resin per column
PTP 92-20-23	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTP 92-20-24	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgC-Fc (ms) resin per column
PTP 92-20-25	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgM resin per column
PTR 41-10-01	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Protein A affinity resin per column
PTR 41-10-02	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Protein G affinity resin per column
PTR 41-10-03	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of IMAC affinity resin per column
PTR 41-10-04	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Glutathione affinity resin per column
PTR 41-10-05	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Streptavidin per column
PTR 41-10-07	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of ProPlus affinty resin per column
PTR 41-10-09	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Normal Phase resin per column
PTR 41-10-11	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Strong Anion resin per column
PTR 41-10-12	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Weak Anion resin per column
PTR 41-10-13	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Strong Cation resin per column
PTR 41-10-14	Box of 48 PhyTip columns (1 mL volume) containing 10 μL of Weak Cation resin per column
PTR 41-16-01	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Protein A affinity resin per column
PTR 41-16-02	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Protein G affinity resin per column
PTR 41-16-03	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of IMAC affinity resin per column
PTR 41-16-04	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Glutathione affinity resin per column
PTR 41-16-05	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Streptavidin per column
PTR 41-16-07	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of ProPlus affinty resin per column
PTR 41-16-11	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Strong Anion resin per column
PTR 41-16-12	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Weak Anion resin per column
PTR 41-16-13	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Strong Cation resin per column
PTR 41-16-14	Box of 48 PhyTip columns (1 mL volume) containing 160 μL of Weak Cation resin per column
PTR 41-20-01	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Protein A affinity resin per column
PTR 41-20-02	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Protein G affinity resin per column
PTR 41-20-03	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of IMAC affinity resin per column
PTR 41-20-04	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Glutathione affinity resin per column
PTR 41-20-05	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Streptavidin per column
PTR 41-20-07	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of ProPlus affinty resin per column
PTR 41-20-09	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Normal Phase resin per column
PTR 41-20-11	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Strong Anion resin per column
PTR 41-20-12	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Weak Anion resin per column
PTR 41-20-13	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Strong Cation resin per column
PTR 41-20-14	Box of 48 PhyTip columns (1 mL volume) containing 20 μL of Weak Cation resin per column
PTR 41-32-01	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Protein A affinity resin per column
PTR 41-32-02	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Protein G affinity resin per column
PTR 41-32-03	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of IMAC affinity resin per column
PTR 41-32-04	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Glutathione affinity resin per column
PTR 41-32-05	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Streptavidin per column
PTR 41-32-07	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of ProPlus affinty resin per column
PTR 41-32-11	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Strong Anion resin per column
PTR 41-32-12	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Weak Anion resin per column
PTR 41-32-13	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Strong Cation resin per column
PTR 41-32-14	Box of 48 PhyTip columns (1 mL volume) containing 320 μL of Weak Cation resin per column
PTR 41-40-01	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Protein A affinity resin per column
PTR 41-40-02	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Protein G affinity resin per column
PTR 41-40-03	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of IMAC affinity resin per column
PTR 41-40-04	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Glutathione affinity resin per column
PTR 41-40-05	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Streptavidin per column
PTR 41-40-07	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of ProPlus affinty resin per column
PTR 41-40-11	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Strong Anion resin per column
PTR 41-40-12	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Weak Anion resin per column
PTR 41-40-13	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Strong Cation resin per column
PTR 41-40-14	Box of 48 PhyTip columns (1 mL volume) containing 40 μL of Weak Cation resin per column
PTR 41-80-01	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Protein A affinity resin per column
PTR 41-80-02	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Protein G affinity resin per column
PTR 41-80-03	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of IMAC affinity resin per column
PTR 41-80-04	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Glutathione affinity resin per column
PTR 41-80-05	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Streptavidin per column
PTR 41-80-07	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of ProPlus affinty resin per column
PTR 41-80-11	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Strong Anion resin per column
PTR 41-80-12	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Weak Anion resin per column
PTR 41-80-13	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Strong Cation resin per column
PTR 41-80-14	Box of 48 PhyTip columns (1 mL volume) containing 80 μL of Weak Cation resin per column
PTR 42-05-01	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Protein A affinity resin per column
PTR 42-05-02	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Protein G affinity resin per column
PTR 42-05-03	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of IMAC affinity resin per column
PTR 42-05-04	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Glutathione affinity resin per column
PTR 42-05-05	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Streptavidin per column
PTR 42-05-07	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of ProPlus affinty resin per column
PTR 42-05-09	Box of 48 PhyTip columns( 200 μL volume) containing 5 μL of Normal Phase resin per column
PTR 42-05-11	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Strong Anion resin per column
PTR 42-05-12	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Weak Anion resin per column
PTR 42-05-13	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Strong Cation resin per column
PTR 42-05-14	Box of 48 PhyTip columns (200 μL volume) containing 5 μL of Weak Cation resin per column
PTR 42-20-01	Box of 48 PhyTip columns (200 μL volume) containing 20 μL of Protein A affinity resin per column
PTR 42-20-02	Box of 48 PhyTip columns (200 μL volume) containing 20 μL of Protein G affinity resin per column
PTR 42-20-03	Box of 48 PhyTip columns (200 μL volume) containing 20 μL of IMAC affinity resin per column
PTR 42-20-04	Box of 48 PhyTip columns (200 μL volume) containing 20 μL of Glutathione affinity resin per column
PTR 42-20-05	Box of 48 PhyTip columns (200 μL volume) containing 20 μL of Streptavidin per column
PTR 42-20-07	Box of 48 PhyTip columns (200 μL volume) containing 20 μL of ProPlus affinty resin per column
PTR 42-20-09	Box of 48 PhyTip columns( 200 μL volume) containing 20 μL of Normal Phase resin per column
PTR 42-20-11	Box of 48 PhyTip columns (200 μL volume) containing 20 μL of Strong Anion resin per column
PTR 42-20-12	Box of 48 PhyTip columns (200 μL volume) containing 20 μL of Weak Anion resin per column
PTR 42-20-13	Box of 48 PhyTip columns (200 μL volume) containing 20 μL of Strong Cation resin per column
PTR 42-20-14	Box of 48 PhyTip columns (200 μL volume) containing 20 μL of Weak Cation resin per column
PTR 91-10-01	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Protein A affinity resin per column
PTR 91-10-02	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Protein G affinity resin per column
PTR 91-10-03	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of IMAC affinity resin per column
PTR 91-10-04	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Glutathione affinity resin per column
PTR 91-10-05	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Streptavidin per column
PTR 91-10-07	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of ProPlus affinty resin per column
PTR 91-10-09	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Normal Phase resin per column
PTR 91-10-11	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Strong Anion resin per column
PTR 91-10-12	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Weak Anion resin per column
PTR 91-10-13	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Strong Cation resin per column
PTR 91-10-14	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of Weak Cation resin per column
PTR 91-10-21	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of CaptureSelect LC-kappa (Hu) resin per column
PTR 91-10-22	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of CaptureSelect LC-lambda (Hu) resin per column
PTR 91-10-23	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTR 91-10-24	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of CaptureSelect IgC-Fc (ms) resin per column
PTR 91-10-25	Box of 96 PhyTip columns (1 mL volume) containing 10 μL of CaptureSelect IgM resin per column
PTR 91-16-01	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Protein A affinity resin per column
PTR 91-16-02	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Protein G affinity resin per column
PTR 91-16-03	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of IMAC affinity resin per column
PTR 91-16-04	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Glutathione affinity resin per column
PTR 91-16-05	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Streptavidin per column
PTR 91-16-07	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of ProPlus affinty resin per column
PTR 91-16-11	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Strong Anion resin per column
PTR 91-16-12	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Weak Anion resin per column
PTR 91-16-13	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Strong Cation resin per column
PTR 91-16-14	Box of 96 PhyTip columns (1 mL volume) containing 160 μL of Weak Cation resin per column
PTR 91-20-01	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Protein A affinity resin per column
PTR 91-20-02	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Protein G affinity resin per column
PTR 91-20-03	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of IMAC affinity resin per column
PTR 91-20-04	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Glutathione affinity resin per column
PTR 91-20-05	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Streptavidin per column
PTR 91-20-07	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of ProPlus affinty resin per column
PTR 91-20-09	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Normal Phase resin per column
PTR 91-20-11	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Strong Anion resin per column
PTR 91-20-12	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Weak Anion resin per column
PTR 91-20-13	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Strong Cation resin per column
PTR 91-20-14	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of Weak Cation resin per column
PTR 91-20-21	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of CaptureSelect LC-kappa (Hu) resin per column
PTR 91-20-22	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of CaptureSelect LC-lambda (Hu) resin per column
PTR 91-20-23	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTR 91-20-24	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of CaptureSelect IgC-Fc (ms) resin per column
PTR 91-20-25	Box of 96 PhyTip columns (1 mL volume) containing 20 μL of CaptureSelect IgM resin per column
PTR 91-32-01	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Protein A affinity resin per column
PTR 91-32-02	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Protein G affinity resin per column
PTR 91-32-03	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of IMAC affinity resin per column
PTR 91-32-04	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Glutathione affinity resin per column
PTR 91-32-05	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Streptavidin per column
PTR 91-32-07	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of ProPlus affinty resin per column
PTR 91-32-11	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Strong Anion resin per column
PTR 91-32-12	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Weak Anion resin per column
PTR 91-32-13	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Strong Cation resin per column
PTR 91-32-14	Box of 96 PhyTip columns (1 mL volume) containing 320 μL of Weak Cation resin per column
PTR 91-40-01	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Protein A affinity resin per column
PTR 91-40-02	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Protein G affinity resin per column
PTR 91-40-03	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of IMAC affinity resin per column
PTR 91-40-04	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Glutathione affinity resin per column
PTR 91-40-05	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Streptavidin per column
PTR 91-40-07	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of ProPlus affinty resin per column
PTR 91-40-11	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Strong Anion resin per column
PTR 91-40-12	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Weak Anion resin per column
PTR 91-40-13	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Strong Cation resin per column
PTR 91-40-14	Box of 96 PhyTip columns (1 mL volume) containing 40 μL of Weak Cation resin per column
PTR 91-80-01	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Protein A affinity resin per column
PTR 91-80-02	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Protein G affinity resin per column
PTR 91-80-03	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of IMAC affinity resin per column
PTR 91-80-04	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Glutathione affinity resin per column
PTR 91-80-05	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Streptavidin per column
PTR 91-80-07	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of ProPlus affinty resin per column
PTR 91-80-11	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Strong Anion resin per column
PTR 91-80-12	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Weak Anion resin per column
PTR 91-80-13	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Strong Cation resin per column
PTR 91-80-14	Box of 96 PhyTip columns (1 mL volume) containing 80 μL of Weak Cation resin per column
PTR 92-05-01	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein A affinity resin per column
PTR 92-05-02	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein G affinity resin per column
PTR 92-05-03	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of IMAC affinity resin per column
PTR 92-05-04	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Glutathione affinity resin per column
PTR 92-05-05	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Streptividin per column
PTR 92-05-07	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of ProPlus affinty resin per column
PTR 92-05-09	Box of 96 PhyTip columns( 200 μL volume) containing 5 μL of Normal Phase resin per column
PTR 92-05-11	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Anion resin per column
PTR 92-05-12	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Anion resin per column
PTR 92-05-13	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Cation resin per column
PTR 92-05-14	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Cation resin per column
PTR 92-05-21	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect LC-kappa (Hu) resin per column
PTR 92-05-22	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect LC-lambda (Hu) resin per column
PTR 92-05-23	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTR 92-05-24	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgC-Fc (ms) resin per column
PTR 92-05-25	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgM resin per column
PTR 92-05-NS
PTR 92-20-01	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein A affinity resin per column
PTR 92-20-02	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein G affinity resin per column
PTR 92-20-03	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of IMAC affinity resin per column
PTR 92-20-04	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Glutathione affinity resin per column
PTR 92-20-05	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Streptavidin per column
PTR 92-20-07	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of ProPlus affinty resin per column
PTR 92-20-09	Box of 96 PhyTip columns( 200 μL volume) containing 20 μL of Normal Phase resin per column
PTR 92-20-11	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Anion resin per column
PTR 92-20-12	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Anion resin per column
PTR 92-20-13	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Cation resin per column
PTR 92-20-14	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Cation resin per column
PTR 92-20-21	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect LC-kappa (Hu) resin per column
PTR 92-20-22	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect LC-lambda (Hu) resin per column
PTR 92-20-23	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTR 92-20-24	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgC-Fc (ms) resin per column
PTR 92-20-25	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgM resin per column
PTT 92-05-01	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein A affinity resin per column
PTT 92-05-02	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein G affinity resin per column
PTT 92-05-03	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of IMAC affinity resin per column
PTT 92-05-04	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Glutathione affinity resin per column
PTT 92-05-05	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Streptavidin per column
PTT 92-05-07	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of ProPlus affinity resin per column
PTT 92-05-09	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Normal Phase resin per column
PTT 92-05-11	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Anion resin per column
PTT 92-05-12	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Anion resin per column
PTT 92-05-13	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Cation resin per column
PTT 92-05-14	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Cation resin per column
PTT 92-05-21	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect LC-kappa (Hu) resin per column
PTT 92-05-22	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect LC-lambda (Hu) resin per column
PTT 92-05-23	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTT 92-05-24	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgC-Fc (ms) resin per column
PTT 92-05-25	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgM resin per column
PTT 92-05-NS	Box of 96 PhyScreen Ni-IMAC Resin Screening Toolkit containing 5ul of 12 Ni-IMAC resins
PTT 92-20-01	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein A affinity resin per column
PTT 92-20-02	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein G affinity resin per column
PTT 92-20-03	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of IMAC affinity resin per column
PTT 92-20-04	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Glutathione affinity resin per column
PTT 92-20-05	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Streptavidin per column
PTT 92-20-07	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of ProPlus affinity resin per column
PTT 92-20-09	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Normal Phase resin per column
PTT 92-20-11	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Anion resin per column
PTT 92-20-12	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Anion resin per column
PTT 92-20-13	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Cation resin per column
PTT 92-20-14	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Cation resin per column
PTT 92-20-21	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect LC-kappa (Hu) resin per column
PTT 92-20-22	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect LC-lambda (Hu) resin per column
PTT 92-20-23	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTT 92-20-24	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgC-Fc (ms) resin per column
PTT 92-20-25	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgM resin per column
PTV 92-20-01	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein A affinity resin per column
PTV 92-20-02	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein G affinity resin per column
PTV 92-20-03	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of IMAC affinity resin per column
PTV 92-20-04	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Glutathione affinity resin per column
PTV 92-20-05	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Streptavidin per column
PTV 92-20-07	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of ProPlus affinity resin per column
PTV 92-20-09	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Normal Phase resin per column
PTV 92-20-11	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Anion resin per column
PTV 92-20-12	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Anion resin per column
PTV 92-20-13	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Cation resin per column
PTV 92-20-14	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Cation resin per column
PTV 92-20-21	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect LC-kappa (Hu) resin per column
PTV 92-20-22	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect LC-lambda (Hu) resin per column
PTV 92-20-23	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTV 92-20-24	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgC-Fc (ms) resin per column
PTV 92-20-25	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of CaptureSelect IgM resin per column
PTZ 92-05-01	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein A affinity resin per column
PTZ 92-05-02	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Protein G affinity resin per column
PTZ 92-05-03	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of IMAC affinity resin per column
PTZ 92-05-04	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Glutathione affinity resin per column
PTZ 92-05-05	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Streptavidin per column
PTZ 92-05-07	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of ProPlus affinity resin per column
PTZ 92-05-09	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Normal Phase resin per column
PTZ 92-05-11	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Anion resin per column
PTZ 92-05-12	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Anion resin per column
PTZ 92-05-13	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Strong Cation resin per column
PTZ 92-05-14	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of Weak Cation resin per column
PTZ 92-05-21	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect LC-kappa (Hu) resin per column
PTZ 92-05-22	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect LC-lambda (Hu) resin per column
PTZ 92-05-23	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgC-Fc (Hu) resin per column
PTZ 92-05-24	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgC-Fc (ms) resin per column
PTZ 92-05-25	Box of 96 PhyTip columns (200 μL volume) containing 5 μL of CaptureSelect IgM resin per column
PTZ 92-05-NS	Box of 96 PhyScreen Ni-IMAC Resin Screening Toolkit containing 5ul of 12 Ni-IMAC resins
PTZ 92-20-01	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein A affinity resin per column
PTZ 92-20-02	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Protein G affinity resin per column
PTZ 92-20-03	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of IMAC affinity resin per column
PTZ 92-20-04	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Glutathione affinity resin per column
PTZ 92-20-05	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Streptavidin per column
PTZ 92-20-07	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of ProPlus affinity resin per column
PTZ 92-20-09	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Normal Phase resin per column
PTZ 92-20-11	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Anion resin per column
PTZ 92-20-12	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Anion resin per column
PTZ 92-20-13	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Strong Cation resin per column
PTZ 92-20-14	Box of 96 PhyTip columns (200 μL volume) containing 20 μL of Weak Cation resin per column

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上海金畔生物科技有限公司是实验试剂一站式采购服务商

1：强大的进口辐射能力，血清、抗体、耗材、大部分限制进口品等。

2：产品种类齐全，经营超过700多品牌，基本涵盖所有生物实验试剂耗材。

3：提供加急服务，货品一般1-2周到货。

4：富有竞争良好的信誉，大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校，ROCHE，阿斯利康、国药、fisher等知药企。

6：我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,phynexus,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等几十家国外公司代理。

7：我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批发，欢迎合作。

PhyNexus PTV 92-20-01说明书

发表于2024年3月24日由上海金畔生物科技有限公司

美国phynexus公司是美国的分子生物学试剂耗材供应商，上海金畔生物科技有限公司为您带来phynexus公司的产品和服务

PhyNexus PTV 92-20-01说明书

PhyNexus开发了一种*的技术，用于工程蛋白和抗体的微量纯化，使研究人员能够定期纯化和富集高达1 mL的样品。PhyTip™色谱柱的*设计使洗脱体积低至10μL，从而产生高达50 x的富集因子，纯化蛋白浓度高达5 mg / ml。

纯化和富集的过程是简单的三步技术，其中首先捕获感兴趣的蛋白质，然后纯化并终富集。整个过程可能需要不到15分钟的时间来生成高浓度的全功能蛋白质，可供进一步分析。

IgG回收率为70-90％
获得高度浓缩的纯化蛋白质
蛋白质A PhyTip柱显示从不同水平的背景蛋白质中提取具有非常高纯度的IgG蛋白质

蛋白质A PhyTip柱显示出功能性抗体（IgG）的高结合亲和力和高容量亲和纯化。

蛋白A柱采用我们专有的双流色谱，可提供完整的抗体结合和高终浓度。PhyTip色谱柱兼容大多数8,12和96通道自动化液体处理机器人，包括 Agilent Technologies， Beckman Coulter， Dynamic Devices， Perkin Elmer， Hamilton和 Tecan。

具有蛋白A的PhyTip色谱柱已针对特定PhyNexus试剂和仪器流速/体积进行了优化，如下所示。使用PhyNexus纯化系统收集该信息。带有蛋白A的所有PhyTip色谱柱都配有推荐的PhyTip缓冲液，包括：捕获缓冲液 – 用于需要添加额外缓冲液以补充样品体积并确保捕获正确pH值的情况。洗涤缓冲液I – 磷酸盐缓冲溶液pH 7.4洗涤缓冲液II – 盐水溶液。注意：没有缓冲能力以确保有效洗脱富集缓冲液 – 用于终洗脱步骤 – 磷酸盐缓冲溶液pH 2.5中和缓冲液。 – Tris缓冲溶液pH 9.0注意：富集缓冲液以4 mL pH 2.5磷酸盐缓冲溶液的形式提供，

含有蛋白A树脂的200多个PhyTip色谱柱：

捕获：通过树脂床以250μL/分钟的流速通过树脂床捕获200μL样品。

纯化：200μLPhyNexus蛋白A洗涤缓冲液I，以500μL/ min的流速通过树脂床两个循环，然后用洗涤缓冲液II进行第二次洗涤，在树脂床上通过流动两个循环速率为500μL/ min。

富集：用10μLPhyNexusProtein A Enrichment Buffer将蛋白质洗脱到溶液中，以500μL/ min的流速通过树脂床5个循环。用3μLPhyNexus蛋白A中和缓冲液中和。

出厂时含有的蛋白G富集缓冲液含有：111mM NaH2PO4,140mM NaCl，14.8mM H3PO4，pH3.0

含有蛋白A树脂的1000多种PhyTip色谱柱：

捕获：通过树脂床以250μL/分钟的流速通过四个循环捕获500μL样品。

富集：用15mL PhyNexus蛋白A富集缓冲液将蛋白质洗脱到溶液中，以500μL/ min的流速通过树脂床5个循环。用5μLPhyNexus蛋白A中和缓冲液中和。

蛋白质APhyTip®色谱柱的性能特征和分析程序

介绍

PhyNexusPhyTip®色谱柱是创新的纯化工具，可从根本上简化各种来源的蛋白质的捕获，纯化和富集。这些净化工具成功的关键是设计保留亲和树脂床的机制，具有小的死体积和大的捕获潜力。一组PhyTipcolumns包含蛋白A，以在特定适条件下特异性结合来自不同来源的抗体（IgG），从而允许去除核酸和其他污染物。在快速洗涤步骤后，用常用的低pH缓冲液容易地洗脱纯化的抗体。该技术允许特别高产率的IgG，这取决于各种条件，并且在非常高浓度的载体蛋白如BSA的存在下提供高度选择性的纯化。PhyTip色谱柱具有*的结合能力，可以结合超过100μg的IgG，并且可以有效回收少至200 ng的IgG – 代表近三个数量级的范围。PhyTip色谱柱非常适用于从杂交瘤上清液中筛选抗体或用于高通量提取和纯化亲和标记蛋白。

绩效和分析程序

IgG以高亲和力和特异性结合蛋白A. 这种相互作用的强度和选择性使蛋白A能够有效地从复杂的蛋白质混合物中纯化IgG。为了检测PhyTip柱与蛋白A树脂的性能，测量了使用1000+蛋白A PhyTip柱的纯化IgG（抗FITC-MAb）的回收百分比。

材料和方法

样品：10μgmFITC-MAb（IgG2a亚类）在0.5ml
含有5mg BSA的PBS中，或
2.含有10％FBS（胎牛血清）的DMEM ，或含有5mg BSA的3.SFM
（无血清培养基）
样品处理：使用以下方案在半自动ME 1000平台上处理所有样品：

（1）捕获：通过将样品通过树脂床进行2个输入/输出循环（体积编程为0.6ml，0.25ml / min）来捕获特定蛋白质。
（2）纯化：通过用0.5mL PBS（洗涤缓冲液I）进行1个输入/输出循环（体积编程为0.6ml，0.5ml / min）洗涤结合的蛋白质/亲和树脂，然后进行1个输入/输出循环，去除未结合的蛋白质（体积为0.6ml，0.5ml / min），用0.5mL盐水溶液（洗涤缓冲液II）。
（3）富集：用15μLpH3.0洗脱缓冲液以4次进/出循环（体积编程为0.1-0.15ml，1ml / min）洗脱IgG2a。洗脱后，加入5μL中和缓冲液。注意，由PhyNexus提供的蛋白A和蛋白G PhyTip柱的当前洗脱缓冲液具有2.5的pH，并且当前的中和缓冲液的pH为9.0。

定量程序

（1）为了制备还原的IgG2a，使15μl终洗脱体积与5μl新制备的TCEP（15μl10mg/ ml TCEP在水中反应，终体积为30μl）反应。

（2）使用HP 1050 HPLC系统将20μl还原的IgG2a注入无孔聚苯乙烯二乙烯基苯反相（C-18）柱中。溶剂A（水中0.1％TFA）和溶剂B（ACN中0.1％TFA）之间的梯度为25％至75％，使用5分钟。检测：214和280nm的UV。

（3）T wo主要IgG2a峰在3.18和3.34分钟左右洗脱。在每种情况下，将这两个峰下面积从（3.3-13.5）min积分，并在214nm处记录相应的峰面积。
（4）将相同反应条件下的TCEP处理的IgG2a标准品加载到柱中，并用作恢复计算的输入或标准。

IgG2a恢复的结果

在DMEM + 10％FBS中的回收率= 77％SFM中的回收率+ 5mg BSA = 64％。

已知载体蛋白如白蛋白诱导免疫应答并有助于产生良好的抗体，因此当产生抗体时它通常以高量存在。因此，任何纯化和富集程序必须能够耐受高浓度的白蛋白。此外，上面显示的结果似乎表明高背景蛋白浓度（如BSA）的存在可能对回收率有益。

在载体蛋白存在下IgG2a回收率的再现性

为了验证载体蛋白对大IgG2a回收率的作用，并证明重现性，从多个样品（n = 4）中从PBS（以及含有5mg BSA的PBS）中纯化IgG2a。结果表明，在500倍过量的BSA浓度存在下，纯化的IgG2a的回收率提高（至少20％或更多），并且该方法也是高度可重复的。

PBS中的回收率= 42.25％（SD = 4.66％）PBS中的回收率+ 5mg BSA = 66.5％（SD = 7.5％）

选择性表现和分析程序

为了表征和验证蛋白A-IgG亲和相互作用的选择性，将5mg BSA作为载体蛋白添加至0.5μg样品体积（333倍过量）中的15μgIgG。使用Protein A PhyTip柱处理样品。