dsDNA BR Assay Kit

dsDNA BR Assay Kit

产品说明书

FAQ

COA

已发表文献

 

dsDNA BR Assay Kit 是一种简便、灵敏、准确、宽范围的双链 DNA(dsDNA BR)荧光定量检测试剂盒,在2~1000 ng 区间具有良好的线性关系。本试剂盒包含荧光检测试剂、缓冲液及相关的 dsDNA BR 标准品,使用前先将荧光检测试剂用缓冲液稀释成工作液,然后加入待测 dsDNA 样品,制作标准曲线后,使用荧光酶标仪或 Qubit®荧光仪进行读数。该产品对常规的污染物如蛋白质、盐类等具有较好的耐受性。

 

组分信息

产品编号

组分名称

浓度

100 T

500 T

12643-A

dsDNA BR Reagent

200×concentrate in DMSO

250 μL

1.25 mL

12643-B

dsDNA BR Buffer

Not applicable

50 mL

250 mL

12643-C

dsDNA BR Standard 1

0 ng/μL in TE buffer

1 mL

5×1 mL

12643-D

dsDNA BR Standard 2

100 ng/μL in TE buffer

1 mL

5×1 mL

 

储存条件

2-8℃避光保存 6 个月,冰袋运输,请注意避免反复冻融。

 

注意事项

1)荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭;

2)检测试剂 dsDNA BR Buffer 略起泡,使用时上下颠倒混匀,避免剧烈震荡;

3)dsDNA BR 标准品,每次使用轻柔震荡或颠倒混匀,瞬时离心数秒收集管盖及管壁上的液体;

4)为保证定量结果的精确,请使用校准后的移液器操作,样品浓度较低时请增大待测样品投入体积;

5)检测工作液请现用现配,当天使用完毕,使用前请用标准品校准。

6)为了您的安全和健康,请穿实验服并戴一次性手套操作;

7)本产品仅用作科研用途!

 

实验步骤

使用 Qubit荧光仪进行 dsDNA BR 定量检测分析

dsDNA BR Assay Kit

1.实验准备

1)在使用前,将试剂盒中的各组分恢复至室温(室温放置约半小时)。检查 dsDNA BR Reagent(组分 A)是否有沉淀,若有沉淀物,可将该试剂至于 37℃水浴锅中温育,并轻柔混匀直至沉淀物完全溶解。

准备足够量 0.5 mL PCR 薄壁管并标注。请勿在 PCR 管侧壁标注,以免影响荧光信号采集。

2.配制检测工作液

在避光塑料容器中,使用 dsDNA BR Buffer 按比例将适量 dsDNA BR Reagent 稀释至 1×(例:取 1 μL dsDNA BR Reagent, 加入 199 μL dsDNA BR Buffer),上下颠倒混匀。工作液现用现配,每次配制检测工作液时要使用洁净的容器。

3.配制待检样品

1)配制待检标准品 1 和标准品 2。取 190 μL 检测工作液至标准品 PCR 管中,分别加入 10 μL dsDNA BR Standard 1 和 dsDNA BR Standard 2 至相应标记的标准品 PCR 管中,轻柔涡旋振荡 2-3 sec,尽量避免气泡产生,瞬时离心数秒。

2)配制待检样品。取 180-199 μL 检测工作液至样本 PCR 管中,分别加入 1-20 μL 待检样本,使 PCR 管中每个样本终体积为 200 μL,轻轻涡旋振荡 2-3 sec,尽量避免气泡产生。

4.检测

1)将所有待检 PCR 管置于室温避光孵育 2 min。

2)按照 Qubit荧光仪的操作说明,选择 dsDNA BR 检测程序,使用待检标准品校正荧光曲线后进行待检样品的浓度测定。

Ver.CN20230524

 

dsDNA BR Assay Kit

暂无内容

dsDNA BR Assay Kit

暂无内容

 

dsDNA BR Assay Kit 是一种简便、灵敏、准确、宽范围的双链 DNA(dsDNA BR)荧光定量检测试剂盒,在2~1000 ng 区间具有良好的线性关系。本试剂盒包含荧光检测试剂、缓冲液及相关的 dsDNA BR 标准品,使用前先将荧光检测试剂用缓冲液稀释成工作液,然后加入待测 dsDNA 样品,制作标准曲线后,使用荧光酶标仪或 Qubit®荧光仪进行读数。该产品对常规的污染物如蛋白质、盐类等具有较好的耐受性。

 

组分信息

产品编号

组分名称

浓度

100 T

500 T

12643-A

dsDNA BR Reagent

200×concentrate in DMSO

250 μL

1.25 mL

12643-B

dsDNA BR Buffer

Not applicable

50 mL

250 mL

12643-C

dsDNA BR Standard 1

0 ng/μL in TE buffer

1 mL

5×1 mL

12643-D

dsDNA BR Standard 2

100 ng/μL in TE buffer

1 mL

5×1 mL

 

储存条件

2-8℃避光保存 6 个月,冰袋运输,请注意避免反复冻融。

 

注意事项

1)荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭;

2)检测试剂 dsDNA BR Buffer 略起泡,使用时上下颠倒混匀,避免剧烈震荡;

3)dsDNA BR 标准品,每次使用轻柔震荡或颠倒混匀,瞬时离心数秒收集管盖及管壁上的液体;

4)为保证定量结果的精确,请使用校准后的移液器操作,样品浓度较低时请增大待测样品投入体积;

5)检测工作液请现用现配,当天使用完毕,使用前请用标准品校准。

6)为了您的安全和健康,请穿实验服并戴一次性手套操作;

7)本产品仅用作科研用途!

 

实验步骤

使用 Qubit荧光仪进行 dsDNA BR 定量检测分析

dsDNA BR Assay Kit

1.实验准备

1)在使用前,将试剂盒中的各组分恢复至室温(室温放置约半小时)。检查 dsDNA BR Reagent(组分 A)是否有沉淀,若有沉淀物,可将该试剂至于 37℃水浴锅中温育,并轻柔混匀直至沉淀物完全溶解。

准备足够量 0.5 mL PCR 薄壁管并标注。请勿在 PCR 管侧壁标注,以免影响荧光信号采集。

2.配制检测工作液

在避光塑料容器中,使用 dsDNA BR Buffer 按比例将适量 dsDNA BR Reagent 稀释至 1×(例:取 1 μL dsDNA BR Reagent, 加入 199 μL dsDNA BR Buffer),上下颠倒混匀。工作液现用现配,每次配制检测工作液时要使用洁净的容器。

3.配制待检样品

1)配制待检标准品 1 和标准品 2。取 190 μL 检测工作液至标准品 PCR 管中,分别加入 10 μL dsDNA BR Standard 1 和 dsDNA BR Standard 2 至相应标记的标准品 PCR 管中,轻柔涡旋振荡 2-3 sec,尽量避免气泡产生,瞬时离心数秒。

2)配制待检样品。取 180-199 μL 检测工作液至样本 PCR 管中,分别加入 1-20 μL 待检样本,使 PCR 管中每个样本终体积为 200 μL,轻轻涡旋振荡 2-3 sec,尽量避免气泡产生。

4.检测

1)将所有待检 PCR 管置于室温避光孵育 2 min。

2)按照 Qubit荧光仪的操作说明,选择 dsDNA BR 检测程序,使用待检标准品校正荧光曲线后进行待检样品的浓度测定。

Ver.CN20230524

 

dsDNA BR Assay Kit

暂无内容

dsDNA BR Assay Kit

暂无内容

Rel Assay Diagnostics 代理


Rel Assay Diagnostics 代理

简要描述:Rel Assay Diagnostics代理,Rel Assay Diagnostics上海代理,Rel Assay Diagnostics北京代理, Rel Assay Diagnostics代理, Rel Assay Diagnostics 代理
上海金畔生物科技有限公司 Rel Assay Diagnostics专业代理,具体产品信息欢迎电询:021-50837765

详细介绍

产品咨询

世界*实验材料供应商 Rel Assay Diagnostics 上海金畔生物为其中国代理, Rel Assay Diagnostics 在一直是行业的*,一直为广大科研客户提供zui为优质的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Rel Assay Diagnostics 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

 Rel Assay Diagnostics 中国代理, Rel Assay Diagnostics 上海代理, Rel Assay Diagnostics 北京代理,Rel Assay Diagnostics 广东代理, Rel Assay Diagnostics 江苏代理Rel Assay Diagnostics 湖北代理,Rel Assay Diagnostics 天津,Rel Assay Diagnostics 黑龙江代理,Rel Assay Diagnostics 内蒙古代理,Rel Assay Diagnostics 吉林代理,Rel Assay Diagnostics 福建代理, Rel Assay Diagnostics 江苏代理, Rel Assay Diagnostics 浙江代理, Rel Assay Diagnostics 四川代理,

 

公司:www.relassay.com

 

Rel Assay Diagnostics成立于1993。公司自1993起就一直在研发和生产临床化学试剂,现已成为一个的检测诊断试剂生产商。

Rel Assay Diagnostics公司拥有生产线的所有。同时有OEM业务的专业厂家。Rel Assay Diagnostics主要进行检测试剂盒的研究与开发,在不久的将来会成为世界科学提供更多的试验参数。

Rel Assay一些产品:
·对氧磷酶测定试剂盒
·芳香酯酶检测试剂盒
·PON1 192Q / R表型
·3代总抗氧化状态(TAS)
·总氧化剂状态(TOS)
·氧化应激指标
·过氧化氢酶
·铜蓝蛋白
·总巯基

 

 

上海金畔生物科技有限公司

AssayBioTech品牌代理

AssayBioTech

简要描述:

自2006年1月成立以来,Assay生物技术一直是全球抗体生产和工程检测技术、荧光染料、猝灭剂、重组蛋白和合成肽的贡献者

自2006年1月成立以来,Assay生物技术一直是抗体生产和工程检测技术、荧光染料、猝灭剂、重组蛋白和合成肽的贡献者。位于加州鈥檚旧金山湾区的中心地带,我们一直致力于满足全球对高质量检测试剂盒和用于医药和学术研究的免疫试剂的需求,这些试剂主要构成医疗保健的基础。

Since our inception in January of 2006, Assay Biotechnology has been a worldwide contributor of industry-leading antibody production and engineering and assay technologies, fluorescent dyes, quenchers, recombinant proteins and synthetic peptides. Located in the heart of California’s San Francisco Bay Area, we have strived to answer the global need for high quality assay kits and immunological reagents used in pharmaceutical and academic research that predominantly formulate the basis of healthcare. 

Advanced Protein Assay等产品价格表

货号 品名 规格 目录价($) 目录价(¥)
AAN01-A Anti-actin antibody (rabbit polyAb) + control 1 x 100 µg $225.00 ¥3,150.00
AAN01-B Anti-actin antibody (rabbit polyAb) + control 3 x 100 µg $545.00 ¥7,630.00
AB07-A Actin protein (biotin, rabbit skeletal muscle) 5 x 20 µg $250.00 ¥3,500.00
AB07-C Actin protein (biotin, rabbit skeletal muscle) 20 x 20 µg $675.00 ¥9,450.00
AB07-D Actin protein (biotin, rabbit skeletal muscle) Large quantities Inquire #VALUE!
ACD03-A Anti-Cdc42 Mouse Monoclonal Antibody  1 x 100 µg $260.00 ¥3,640.00
ACD03-B Anti-Cdc42 Mouse Monoclonal Antibody  3 x 100 µg $650.00 ¥9,100.00
ACFL02-A Cofilin antibody (host rabbit) + control 1 x 50 µg $260.00 ¥3,640.00
ACFL02-B Cofilin antibody (host rabbit) + control 3 x 50 µg $650.00 ¥9,100.00
AD99-A Actin protein (bovine cardiac muscle, >99% pure) 1 x 1 mg $90.00 ¥1,260.00
AD99-B Actin protein (bovine cardiac muscle, >99% pure) 5 x 1 mg $395.00 ¥5,530.00
AD99-C Actin protein (bovine cardiac muscle, >99% pure) 20 x 1 mg $1,450.00 ¥20,300.00
ADV01-A Advanced Protein Assay (5X conc) 1 x 500 ml $170.00 ¥2,380.00
ADV01-B Advanced Protein Assay (5X conc) 3 x 500 ml $465.00 ¥6,510.00
ADV02-A Precision Red Advanced Protein Assay (1X conc) 1 x 500 ml $105.00 ¥1,470.00
ADV02-B Precision Red Advanced Protein Assay (1X conc) 3 x 500 ml $295.00 ¥4,130.00
AFB01-A Fibrillarin monoclonal antibody 1 x 100 µg $285.00 ¥3,990.00
AFB01-B Fibrillarin monoclonal antibody 3 x 100 µg $650.00 ¥9,100.00
AKF99-A Pre-formed actin filaments (rabbit skeletal muscle) 1 x 1 mg $155.00 ¥2,170.00
AKF99-B Pre-formed actin filaments (rabbit skeletal muscle) 5 x 1 mg $615.00 ¥8,610.00
AKF99-C Pre-formed actin filaments (rabbit skeletal muscle) 20 x 1 mg $1,650.00 ¥23,100.00
AKIN01-A Kinesin motor antibody (host rabbit + control) 1 x 50 µg $285.00 ¥3,990.00
AKIN01-B Kinesin motor antibody (host rabbit + control) 3 x 50 µg $650.00 ¥9,100.00
AKIN04-A CenPE motor antibody (host rabbit) + control 1 x 50 µg $285.00 ¥3,990.00
AKIN04-B CenPE motor antibody (host rabbit) + control 3 x 50 µg $650.00 ¥9,100.00
AKIN05-A MCAK motor antibody (host rabbit) + control 1 x 50 µg $285.00 ¥3,990.00
AKIN05-B MCAK motor antibody (host rabbit) + control 3 x 50 µg $650.00 ¥9,100.00
AKIN06-A MKLP1 motor antibody (host rabbit) + control 1 x 50 µg $285.00 ¥3,990.00
AKIN06-B MKLP1 motor antibody (host rabbit) + control 3 x 50 µg $650.00 ¥9,100.00
AKIN09-A KIF3C motor antibody (host rabbit) + control 1 x 50 µg $285.00 ¥3,990.00
AKIN09-B KIF3C motor antibody (host rabbit) + control 3 x 50 µg $650.00 ¥9,100.00
AKIN11-A KIF1C motor antibody (host rabbit) + control 1 x 50 µg $285.00 ¥3,990.00
AKIN11-B KIF1C motor antibody (host rabbit) + control 3 x 50 µg $650.00 ¥9,100.00
AKIN12-A Kid motor antibody (host rabbit) + control 1 x 50 µg $285.00 ¥3,990.00
AKIN12-B Kid motor antibody (host rabbit) + control 3 x 50 µg $650.00 ¥9,100.00
AKIN13-A Klp2 motor antibody (host rabbit) + control 1 x 50 µg $285.00 ¥3,990.00
AKIN13-B Klp2 motor antibody (host rabbit) + control 3 x 50 µg $650.00 ¥9,100.00
AKL95-B Actin protein (rabbit skeletal muscle, >95% pure) 1 x 1  mg $75.00 ¥1,050.00
AKL95-C Actin protein (rabbit skeletal muscle, >95% pure) 5 x 1  mg $345.00 ¥4,830.00
AKL95-D Actin protein (rabbit skeletal muscle, >95% pure) 10 x 1 mg $625.00 ¥8,750.00
AKL95-E Actin protein (rabbit skeletal muscle, >95% pure) 20 x 1 mg $1,075.00 ¥15,050.00
AKL95-XL Actin protein (rabbit skeletal muscle, >95% pure) Large quantities Inquire #VALUE!
AKL99-A Actin protein (rabbit skeletal muscle, >99% pure) 4 x 250 µg $180.00 ¥2,520.00
AKL99-B Actin protein (rabbit skeletal muscle, >99% pure) 2 x 1 mg $205.00 ¥2,870.00
AKL99-C Actin protein (rabbit skeletal muscle, >99% pure) 5 x 1 mg $395.00 ¥5,530.00
AKL99-D Actin protein (rabbit skeletal muscle, >99% pure) 10 x 1 mg $725.00 ¥10,150.00
AKL99-E Actin protein (rabbit skeletal muscle, >99% pure) 20 x 1 mg $1,325.00 ¥18,550.00
AKL99-XL Actin protein (rabbit skeletal muscle, >99% pure) Large quantities Inquire #VALUE!
AP05-A Actin protein (pyrene, rabbit skeletal muscle) 1 x 1 mg $185.00 ¥2,590.00
AP05-B Actin protein (pyrene, rabbit skeletal muscle) 5 x 1 mg $685.00 ¥9,590.00
AP05-XL Actin protein (pyrene, rabbit skeletal muscle) Large quantities Inquire #VALUE!
APHL99-A Actin protein (human plaet non-muscle, >99% pure) 2 x 250 µg $170.00 ¥2,380.00
APHL99-C Actin protein (human plaet non-muscle, >99% pure) 1 x 1 mg $245.00 ¥3,430.00
APHL99-E Actin protein (human plaet non-muscle, >99% pure) 5 x 1 mg $850.00 ¥11,900.00
APHL99-XL Actin protein (human plaet non-muscle, >99% pure) Large quantities Inquire #VALUE!
APHR-A Actin protein (rhodamine, human plaet non-muscle) 4 x 10 µg $245.00 ¥3,430.00
APHR-C Actin protein (rhodamine, human plaet non-muscle) 20 x 10 µg $750.00 ¥10,500.00
APHR-D Actin protein (rhodamine, human plaet non-muscle) Large quantities Inquire #VALUE!
APUF01-A Profilin polyclonal antibody (host rabbit) + control 1 x 50 µg $245.00 ¥3,430.00
APUF01-B Profilin polyclonal antibody (host rabbit) + control 3 x 50 µg $595.00 ¥8,330.00
AR05-B Actin protein (rhodamine, rabbit skeletal muscle) 10 x 20 µg $225.00 ¥3,150.00
AR05-C Actin protein (rhodamine, rabbit skeletal muscle) 20 x 20 µg $435.00 ¥6,090.00
AR05-D Actin protein (rhodamine, rabbit skeletal muscle) Large quantities Inquire #VALUE!
ARC03-A Anti-Rac1 specific mouse MAb 100 µg $285.00 ¥3,990.00
ARC03-B Anti-Rac1 specific mouse MAb 300 µg $650.00 ¥9,100.00
ARH03-A Anti-RhoA antibody (mouse MAb) + control. 100 µg $225.00 ¥3,150.00
ARH03-B Anti-RhoA antibody (mouse MAb) + control. 300 µg $495.00 ¥6,930.00
AS99-A Actin protein (chicken gizzard muscle, >99% pure) 1 x 1 mg $90.00 ¥1,260.00
AS99-B Actin protein (chicken gizzard muscle, >99% pure) 5 x 1 mg $395.00 ¥5,530.00
AS99-C Actin protein (chicken gizzard muscle, >99% pure) 20 x 1 mg $1,450.00 ¥20,300.00
AT01-A Alpha-actinin (rabbit skeletal muscle) 2 x 50 µg $235.00 ¥3,290.00
AT01-C Alpha-actinin (rabbit skeletal muscle) 10 x 50 µg $775.00 ¥10,850.00
ATN02-A Anti-tubulin polyAb pan specific (sheep) + control 1 x 100 µg $125.00 ¥1,750.00
ATN02-B Anti-tubulin polyAb pan specific (sheep) + control 3 x 100 µg $295.00 ¥4,130.00
BK001 Actin Binding Protein Spin-Down Assay Kit KIT 30-100 assays $625.00 ¥8,750.00
BK003 Actin Polymerization Biochem Kit  KIT 30-100 assays $695.00 ¥9,730.00
BK004P / CDS01P Tubulin Polymerization Assay Kit (>97% pure porcine brain tubulin) KIT 24 assays $585.00 ¥8,190.00
BK005 F-Actin Visualization Kit KIT 300 assays $325.00 ¥4,550.00
BK006P / CDS03P Tubulin Polymerization Assay Kit (>99% pure porcine brain tubulin) KIT 24 assays $850.00 ¥11,900.00
BK008 Ras Activation Assay Biochem Kit KIT 50 assays $670.00 ¥9,380.00
BK008-S Ras Activation Assay Biochem Kit KIT 20 assays $450.00 ¥6,300.00
BK010S Plant Tubulin Polymerization Kit (soybean tubulin) KIT 30 assays $1,850.00 ¥25,900.00
BK011P Tubulin Polymerization Assay (fluoresc-96assays porcine brain tubulin) KIT 96 assays $850.00 ¥11,900.00
BK013 Actin Binding Protein Spin-Down Assay Kit (Non-muscle) KIT 30-100 assays $675.00 ¥9,450.00
BK029 Microtubule Binding Protein Spin Down Assay Kit KIT 30-100 assays $625.00 ¥8,750.00
BK030 Combo RhoA/Rac1/Cdc42 Activation Assay Kit KIT 3 x 10 assays $650.00 ¥9,100.00
BK032-S Arf1 Pulldown Activation Assay Kit KIT 20 assays $450.00 ¥6,300.00
BK033-S Arf6 Pulldown Activation Assay Kit KIT 20 assays $450.00 ¥6,300.00
BK034 Cdc42 Pulldown Activation Assay Kit KIT 50 assays $670.00 ¥9,380.00
BK034-S Cdc42 Pulldown Activation Assay Kit KIT 20 assays $450.00 ¥6,300.00
BK035 Rac1 Pulldown Activation Assay Kit KIT 50 assays $670.00 ¥9,380.00
BK035-S Rac1 Pulldown Activation Assay Kit KIT 20 assays $450.00 ¥6,300.00
BK036 RhoA Pulldown Activation Assay Kit KIT 80 assays $670.00 ¥9,380.00
BK036-S RhoA Pulldown Activation Assay Kit KIT 20 assays $450.00 ¥6,300.00
BK037 G-Actin : F-Actin In Vivo Assay Kit KIT 30-100 assays $650.00 ¥9,100.00
BK038 Microtubule / Tubulin In Vivo Assay Kit KIT 30-100 assays $650.00 ¥9,100.00
BK040 RalA Activation Assay Biochem Kit KIT 50  assays $670.00 ¥9,380.00
BK051 ATPase ELIPA™ (Enzyme Linked Inorganic Phosphate Assay) KIT 96 assays $255.00 ¥3,570.00
BK052 GTPase ELIPA™ (Enzyme Linked Inorganic Phosphate Assay) KIT 96 assays $255.00 ¥3,570.00
BK053 HTS Kinesin ATPase Endpoint Assay KIT 1000 assays $685.00 ¥9,590.00
BK054 CytoPhos™ Phosphate Assay (1-500ug/ml protein reactions) KIT 1000 assays $255.00 ¥3,570.00
BK060 Kinesin ELIPA (Enzyme Linked Inorganic Phosphate Assay TM) KIT 96 assays $675.00 ¥9,450.00
BK100 GEF Exchange Assay KIT 60-300 assays $585.00 ¥8,190.00
BK105 GAP Assay Kit KIT 80-160 assays $585.00 ¥8,190.00
BK121 RhoA G-LISA Activation Assay (luminescence) KIT 96 assays $760.00 ¥10,640.00
BK124 RhoA G-LISA Activation Assay (absorbance) KIT 96 assays $760.00 ¥10,640.00
BK125 Rac1,2,3 G-LISA Activation Assay Kit KIT 96 assays $760.00 ¥10,640.00
BK126 Rac1 G-LISA Activation Assay Kit KIT 96 assays $760.00 ¥10,640.00
BK127 Cdc42 G-LISA Activation Assay Kit KIT 96 assays $760.00 ¥10,640.00
BK128 Rac1 G-LISA Activation Assay Kit KIT 96 assays $760.00 ¥10,640.00
BK129 RalA G-LISA Activation Assay Kit KIT 96 assays $760.00 ¥10,640.00
BK131 Ras G-LISA Activation Assay Kit KIT 96 assays $760.00 ¥10,640.00
BK132 Arf1 G-LISA Activation Assay Kit KIT 96 assays $760.00 ¥10,640.00
BK133 Arf6 G-LISA Activation Assay Kit KIT 96 assays $760.00 ¥10,640.00
BK150 RhoA ELISA Kit KIT 96 assays $395.00 ¥5,530.00
BM01-A BimC Motor Domain (Asp.nid.) 2 x 25 µg $299.00 ¥4,186.00
BM01-B BimC Motor Domain (Asp.nid.) 10 x 25 µg $995.00 ¥13,930.00
BM01-XL BimC Motor Domain (Asp.nid.)  1 x 1 mg $1,695.00 ¥23,730.00
BS01 GTP gamma S 1 x 500 µg $85.00 ¥1,190.00
BSA01-001 General Actin Buffer 1 x 10 ml $56.00 ¥784.00
BSA01-010 General Actin Buffer 1 x 100 ml $150.00 ¥2,100.00
BSA02-001 Actin Polymerization Buffer (10x stock) 1 x 2 ml $56.00 ¥784.00
BSA04-001 ATP (100mM Stock) 1 x 1 ml $56.00 ¥784.00
BST01-001 General Tubulin Buffer 1 x 10 ml $56.00 ¥784.00
BST01-010 General Tubulin Buffer 1 x 100 ml $135.00 ¥1,890.00
BST01-100 General Tubulin Buffer 1 x 1000 ml $350.00 ¥4,900.00
BST05-001 Tubulin Glycerol Buffer 1 x 10 ml $56.00 ¥784.00
BST06-001 GTP (100mM Stock) 1 x 100 µl $56.00 ¥784.00
BST06-010 GTP (100mM Stock) 10 x 100 µl $180.00 ¥2,520.00
C17G01-A Cdc42-GST protein: dominant negative 1 x 25 µg  $235.00 ¥3,290.00
C17G01-C Cdc42-GST protein: dominant negative 4 x 25 µg $570.00 ¥7,980.00
C6101-A Cdc42-His Protein: constituitively active 1 x 10 µg $235.00 ¥3,290.00
C6101-C Cdc42-His Protein: constituitively active 4 x 10 µg $570.00 ¥7,980.00
CD01-A Cdc42-His Protein: Wild type 1 x 100 µg $235.00 ¥3,290.00
CD01-C Cdc42-His Protein: Wild type 3 x 100 µg $570.00 ¥7,980.00
CD01-XL Cdc42-His Protein: Wild type 1 x 1 mg $980.00 ¥13,720.00
CDG01-C Cdc42-GST Protein: Wild type 8 x 25 µg $435.00 ¥6,090.00
CF01-A Cofilin (Human Recombinant) 1 x 100 µg $215.00 ¥3,010.00
CF01-C Cofilin (Human Recombinant) 4 x 100 µg $695.00 ¥9,730.00
CN01-A Rho Activator 5 x 10 units $195.00 ¥2,730.00
CN01-B Rho Activator 20 x 10 units $665.00 ¥9,310.00
CN02-A Rac and Cdc42 Activator 5 x 10 units $195.00 ¥2,730.00
CN02-B Rac and Cdc42 Activator 20 x 10 units $665.00 ¥9,310.00
CN03-A Rho activator II 3 x 20 µg $195.00 ¥2,730.00
CN03-B Rho activator II 9 x 20 µg $525.00 ¥7,350.00
CN04-A Rho/Rac/Cdc42 Activator I 3 x 20 µg $195.00 ¥2,730.00
CN04-B Rho/Rac/Cdc42 Activator I 9 x 20 µg $525.00 ¥7,350.00
CN06-A Rho Pathway Inhibitor I 5 x 10 units $70.00 ¥980.00
CN06-B Rho Pathway Inhibitor I 20 x 10 units $215.00 ¥3,010.00
CP01-A CENP-E Motor domain (Hm.sap.) 2 x 25 µg $299.00 ¥4,186.00
CP01-B CENP-E Motor domain (Hm.sap.) 10 x 25 µg $995.00 ¥13,930.00
CP01-XL CENP-E Motor domain (Hm.sap.) (Bulk discount avail.) 1 x 1 mg $1,695.00 ¥23,730.00
CR01-A Chromokinesin Motor Domain (Hm.sap.) 2 x 25 µg $299.00 ¥4,186.00
CR01-B Chromokinesin Motor Domain (Hm.sap.) 10 x 25 µg $995.00 ¥13,930.00
CT03-A C3 transferase protein 1 x 25 µg $195.00 ¥2,730.00
CT03-B C3 transferase protein 2 x 25 µg $395.00 ¥5,530.00
CT03-C C3 transferase protein 4 x 25 µg $635.00 ¥8,890.00
CT04-A Cell Permeable Rho Inhibitor (C3 Trans based)) 1 x 20 µg $195.00 ¥2,730.00
CT04-B Cell Permeable Rho Inhibitor (C3 Trans based)) 5 x 20 µg $545.00 ¥7,630.00
CT04-C Cell Permeable Rho Inhibitor (C3 Trans based)) 20 x 20 µg $1,450.00 ¥20,300.00
EG01-A Eg5 Motor Domain (Hm.sap.) 2 x 25 µg $299.00 ¥4,186.00
EG01-B Eg5 Motor Domain (Hm.sap.) 10 x 25 µg $995.00 ¥13,930.00
EG01-XL Eg5 Motor Domain (Hm.sap.) (Bulk discount avail.) 1 x 1 mg $1,695.00 ¥23,730.00
EG02-A Eg5 Homolog Motor Domain (Asp.fum.) 2 x 15 µg $299.00 ¥4,186.00
EG02-B Eg5 Homolog Motor Domain (Asp.fum.) 10 x 15 µg $995.00 ¥13,930.00
EG02-XL Eg5 Homolog Motor Domain (Asp.fum.) 1 x 1 mg $1,695.00 ¥23,730.00
F001 Fungal Cell Tubulin (Agaricus bisporus) 1 x 250 µg $755.00 ¥10,570.00
FNR01-A Fibronectin Rhodamine (bovine plasma) 5 x 20 µg $250.00 ¥3,500.00
FNR01-B Fibronectin Rhodamine (bovine plasma) 20 x 20 µg $750.00 ¥10,500.00
FNR02-A Fibronectin HiLyte488™ (bovine plasma) 5 x 20 µg $250.00 ¥3,500.00
FNR02-B Fibronectin HiLyte488™ (bovine plasma) 20 x 20 µg $750.00 ¥10,500.00
FNR03-A Fibronectin Biotin (bovine plasma) 5 x 20 µg $250.00 ¥3,500.00
FNR03-B Fibronectin Biotin (bovine plasma) 20 x 20 µg $750.00 ¥10,500.00
FTZ01-A FtsZ Protein (E.coli  recomb. no tag) 1 x 1 mg $350.00 ¥4,900.00
FTZ01-B FtsZ Protein (E.coli  recomb. no tag) 5 x 1 mg $1,450.00 ¥20,300.00
FTZ02-A FtsZ Protein (Staphylococcus aureus recombinant plus 6xHis tag) 1 x 1 mg $350.00 ¥4,900.00
FTZ02-B FtsZ Protein (Staphylococcus aureus recombinant plus 6xHis tag) 5 x 1 mg $1,450.00 ¥20,300.00
FTZ03-A FtsZ Protein (Streptococcus pneumoniae recombinant plus 6xHis tag) 1 x 1 mg $350.00 ¥4,900.00
FTZ03-B FtsZ Protein (Streptococcus pneumoniae recombinant plus 6xHis tag) 5 x 1 mg $1,450.00 ¥20,300.00
FTZ04-A FtsZ Protein (Enterococcus faecalis recombinant plus 6xHis tag) 1 x 1 mg $350.00 ¥4,900.00
FTZ04-B FtsZ Protein (Enterococcus faecalis recombinant plus 6xHis tag) 5 x 1 mg $1,450.00 ¥20,300.00
FTZ05-A FtsZ Protein (E.coli  recombinant, 6xHis-tagged) 1 x 1 mg $350.00 ¥4,900.00
FTZ05-B FtsZ Protein (E.coli  recombinant, 6xHis-tagged) 5 x 1 mg $1,450.00 ¥20,300.00
GAP01-A Rho GAP Protein GST 1 x 50 µg $230.00 ¥3,220.00
GAP01-B Rho GAP Protein GST 4 x 50 µg $600.00 ¥8,400.00
GAS01-A Rho GAP Protein: catalytic domain GST 1 x 50 µg $230.00 ¥3,220.00
GAS01-B Rho GAP Protein: catalytic domain GST 4 x 50 µg $600.00 ¥8,400.00
GDI01-A GDP dissociation inhibitor protein GST fusion 1 x 25 µg $230.00 ¥3,220.00
GDI01-B GDP dissociation inhibitor protein GST fusion 4 x 25 µg $600.00 ¥8,400.00
GE01-A Dbs protein GEF domain – HIS fusion 2 x 50 µg $230.00 ¥3,220.00
GE01-B Dbs protein GEF domain – HIS fusion 8 x 50 µg $600.00 ¥8,400.00
H001-B Hela Cancer Cell Tubulin 1 x 250 µg $670.00 ¥9,380.00
H003 Hela Cancer Cell Tubulin Biotinylated 1 x 40 µg $570.00 ¥7,980.00
H005 MCF-7 Cancer Cell Tubulin 1 x 250 µg $670.00 ¥9,380.00
HPG6-A Gelsolin (recomb. Human) 4 x 20 µg $265.00 ¥3,710.00
HPG6-B Gelsolin (recomb. Human) 20 x 20 µg $850.00 ¥11,900.00
HTS03-A Tubulin (HTS format, porcine, 8 assays) 1 x 4 mg $260.00 ¥3,640.00
HTS03-B Tubulin (HTS format, porcine, 96 assays) 1 x 40 mg $1,370.00 ¥19,180.00
HTS03-XL Tubulin (HTS format, porcine, 250 assays) 1 x 100 mg $2,730.00 ¥38,220.00
KC01-A KIFC3 Motor Domain (Hm.sap) 2 x 25 µg $299.00 ¥4,186.00
KC01-B KIFC3 Motor Domain (Hm.sap) 10 x 25 µg $995.00 ¥13,930.00
KF01-A KIF3C Motor Domain (Hm.sap.) 2 x 25 µg $299.00 ¥4,186.00
KF01-B KIF3C Motor Domain (Hm.sap.) 10 x 25 µg $995.00 ¥13,930.00
KF01-XL KIF3C Motor Domain (Hm.sap.) (Bulk discounts avail.) 1 x 1 mg $1,695.00 ¥23,730.00
KR01-A KHC Motor Domain (Hm.sap.) 2 x 25 µg $299.00 ¥4,186.00
KR01-B KHC Motor Domain (Hm.sap.) 10 x 25 µg $995.00 ¥13,930.00
KR01-XL KHC Motor Domain (Hm.sap.) (Bulk discounts avail.) 1 x 1 mg $1,695.00 ¥23,730.00
LMN01-A Laminin Rhodamine (mouse tumor) 5 x 20 µg $250.00 ¥3,500.00
LMN01-B Laminin Rhodamine (mouse tumor) 20 x 20 µg $750.00 ¥10,500.00
LMN02-A Laminin HiLyte488™ (mouse tumor) 5 x 20 µg $250.00 ¥3,500.00
LMN02-B Laminin HiLyte488™ (mouse tumor) 20 x 20 µg $750.00 ¥10,500.00
LMN03-A Laminin Biotin (mouse tumor) 5 x 20 µg $250.00 ¥3,500.00
LMN03-B Laminin Biotin (mouse tumor) 20 x 20 µg $750.00 ¥10,500.00
MAPF-A MAP Fraction 1 x 100 µg $195.00 ¥2,730.00
MAPF-C MAP Fraction 5 x 100 µg $695.00 ¥9,730.00
MH01-A Myosin: Heavy Meromyosin 4 x 50 µg $235.00 ¥3,290.00
MH01-B Myosin: Heavy Meromyosin 20 x 50 µg $670.00 ¥9,380.00
MK01-A MCAK Motor Domain (Hm.sap.) 2 x 25 µg $299.00 ¥4,186.00
MK01-B MCAK Motor Domain (Hm.sap.) 10 x 25 µg $995.00 ¥13,930.00
MK01-XL MCAK Motor Domain (Hm.sap.)  1 x 1 mg $1,695.00 ¥23,730.00
ML116-A Tubulin: MAP-rich (porcine) 1 x 1 mg $99.00 ¥1,386.00
ML116-B Tubulin: MAP-rich (porcine) 5 x 1 mg $425.00 ¥5,950.00
ML116-C Tubulin: MAP-rich (porcine) 20 x 1 mg $1,170.00 ¥16,380.00
ML116-DX Tubulin: MAP-rich (porcine) 1 x 10 mg $645.00 ¥9,030.00
ML116-XL Tubulin: MAP-rich (porcine) Large quantities Inquire #VALUE!
MP01-A MKLP Motor Domain (Hm.sap.) 2 x 25 µg $299.00 ¥4,186.00
MP01-B MKLP Motor Domain (Hm.sap.) 10 x 25 µg $995.00 ¥13,930.00
MP01-XL MKLP Motor Domain (Hm.sap.)  1 x 1 mg $1,695.00 ¥23,730.00
MT001-A Microtubules Pre-formed Motor ATPase ready (bovine) 4 x 500 µg $325.00 ¥4,550.00
MT001-XL Microtubules Pre-formed Motor ATPase ready (bovine) 1 x 10 mg $1,130.00 ¥15,820.00
MT002-A Microtubules Pre-formed Motor ATPase ready(porcine) 4 x 500 µg $325.00 ¥4,550.00
MT002-XL Microtubules Pre-formed Motor ATPase ready(porcine) 1 x 10 mg $1,130.00 ¥15,820.00
MY02-A Myosin Skeletal Muscle 5 x 1 mg $180.00 ¥2,520.00
MY02-B Myosin Skeletal Muscle 20 x 1 mg $670.00 ¥9,380.00
MY03-A Myosin Cardiac Muscle 5 x 1 mg $350.00 ¥4,900.00
MY03-B Myosin Cardiac Muscle 10 x 1 mg $670.00 ¥9,380.00
PAK01-A PAK Rac1/Cdc42 binding domain protein 1 x 250 µg $325.00 ¥4,550.00
PAK01-B PAK Rac1/Cdc42 binding domain protein 4 x 250 µg $930.00 ¥13,020.00
PAK02-A PAK Beads, Rac1/Cdc42 binding domain protein on beads 1 x 500 µg $395.00 ¥5,530.00
PAK02-B PAK Beads, Rac1/Cdc42 binding domain protein on beads 4 x 500 µg $1,185.00 ¥16,590.00
PHDG1-A Acti-stain™ 488 phalloidin 1 x 500 µl (300 slides) $145.00 ¥2,030.00
PHDH1-A Acti-stain™ 555 phalloidin 1 x 500 µl (300 slides) $145.00 ¥2,030.00
PHDN1-A Acti-stain™ 670 phalloidin 1 x 500 µl (300 slides) $145.00 ¥2,030.00
PHDR1 Rhodamine Phalloidin (14uM in methanol) 1 x 500 µl $125.00 ¥1,750.00
PIC02 Protease Inhibitor Cocktail (100x strength) 1 x 1 ml $60.00 ¥840.00
PR01-A Profilin 1 (recombinant human) 1 x 50 µg $225.00 ¥3,150.00
PR01-C Profilin 1 (recombinant human) 4 x 50 µg $650.00 ¥9,100.00
R17G01-A Rac1 GST Protein: dominant negative 1 x 25 µg $235.00 ¥3,290.00
R17G01-C Rac1 GST Protein: dominant negative 4 x 25 µg $570.00 ¥7,980.00
R6101-A Rac1 His Protein: constitutively active 1 x 10 µg $235.00 ¥3,290.00
R6101-C Rac1 His Protein: constitutively active 4 x 10 µg $570.00 ¥7,980.00
R6301-A RhoA His Protein: constitutively active 1 x 10 µg $235.00 ¥3,290.00
R6301-C RhoA His Protein: constitutively active 4 x 10 µg $570.00 ¥7,980.00
RC01-A Rac1 His Protein: wild type 1 x 100 µg $235.00 ¥3,290.00
RC01-C Rac1 His Protein: wild type 3 x 100 µg $570.00 ¥7,980.00
RC01-XL Rac1 His Protein: wild type 1 x 1 mg $1,250.00 ¥17,500.00
RC02-A Rac2 His Protein: wild type 1 x 100 µg $235.00 ¥3,290.00
RC02-B Rac2 His Protein: wild type 3 x 100 µg $570.00 ¥7,980.00
RCG01-C Rac1 GST Protein: wild type 8 x 25 µg $435.00 ¥6,090.00
RF02-A Raf protein ras binding domain on GST beads 1 x 2 mg $395.00 ¥5,530.00
RF02-B Raf protein ras binding domain on GST beads 4 x 2 mg $1,185.00 ¥16,590.00
RH01-A RhoA His Protein: wild type 1 x 100 µg $235.00 ¥3,290.00
RH01-C RhoA His Protein: wild type 3 x 100 µg $570.00 ¥7,980.00
RH01-XL RhoA His Protein: wild type 1 x 1 mg $1,250.00 ¥17,500.00
RH03-A RhoC His Protein: wild type 1 x 100 µg $235.00 ¥3,290.00
RH03-C RhoC His Protein: wild type 3 x 100 µg $570.00 ¥7,980.00
RHG01-C RhoA GST Protein: wild type 8 x 25 µg $435.00 ¥6,090.00
RN01-A Ran protein (Human wild type, His) 1 x 10 µg $235.00 ¥3,290.00
RN01-B Ran protein (Human wild type, His) 3 x 10 µg $570.00 ¥7,980.00
RN03-A Ran protein (Human Q69L mutant, His) 1 x 10 µg $235.00 ¥3,290.00
RN03-B Ran protein (Human Q69L mutant, His) 3 x 10 µg $570.00 ¥7,980.00
RN05-A Ran protein (Human T24N mutant, His) 1 x 10 µg $235.00 ¥3,290.00
RN05-B Ran protein (Human T24N mutant, His) 3 x 10 µg $570.00 ¥7,980.00
RN07-A Ran BP1 protein (Xenopus wild type, His) 1 x 250 µg $280.00 ¥3,920.00
RN07-B Ran BP1 protein (Xenopus wild type, His) 3 x 250 µg $640.00 ¥8,960.00
RP01-A Arp2/3 Protein Complex (Bovine brain) 2 x 50 µg $375.00 ¥5,250.00
RP01-B Arp2/3 Protein Complex (Bovine brain) 6 x 50 µg $975.00 ¥13,650.00
RR02-A Rap1b protein (Human wild type, His) 1 x 100 µg $235.00 ¥3,290.00
RR02-B Rap1b protein (Human wild type, His) 3 x 100 µg $570.00 ¥7,980.00
RS01-A H-Ras-His Protein wild type 1 x 100 µg $235.00 ¥3,290.00
RS01-C H-Ras-His Protein wild type 3 x 100 µg $570.00 ¥7,980.00
RT01-A Rhotekin RBD protein GST fusion 1 x 500 µg $380.00 ¥5,320.00
RT01-B Rhotekin RBD protein GST fusion 3 x 500 µg $980.00 ¥13,720.00
RT02-A Rhotekin RBD protein on GST beads 2 x 2 mg $475.00 ¥6,650.00
RT02-B Rhotekin RBD protein on GST beads 6 x 2 mg $1,130.00 ¥15,820.00
T238P-A Tubulin (porcine brain, >99% pure) 1 x 1 mg $150.00 ¥2,100.00
T238P-B Tubulin (porcine brain, >99% pure) 5 x 1 mg $595.00 ¥8,330.00
T238P-C Tubulin (porcine brain, >99% pure) 20 x 1 mg $1,695.00 ¥23,730.00
T240-A Tubulin (porcine brain, >99% pure) 1 x 1 mg $110.00 ¥1,540.00
T240-B Tubulin (porcine brain, >99% pure) 5 x 1 mg $495.00 ¥6,930.00
T240-C Tubulin (porcine brain, >99% pure) 20 x 1 mg $1,685.00 ¥23,590.00
T240-DX Tubulin (porcine brain, >99% pure) 1 x 10 mg $825.00 ¥11,550.00
T240-XL Tubulin (porcine brain, >99% pure) Large quantities Inquire #VALUE!
T333P-A Tubulin (Biotin Conjugate; Porcine Brain) 5 x 20 µg $275.00 ¥3,850.00
T333P-B Tubulin (Biotin Conjugate; Porcine Brain) 20 x 20 µg $735.00 ¥10,290.00
T333P-XL Tubulin (Biotin Conjugate; Porcine Brain) 1 x 500 µg $630.00 ¥8,820.00
TA01-A Tau (bovine brain) 1 x 50 µg $260.00 ¥3,640.00
TA01-B Tau (bovine brain) 3 x 50 µg $610.00 ¥8,540.00
TL238-A Tubulin (bovine brain, >99% pure, lyophilized) 4 x 250 µg $170.00 ¥2,380.00
TL238-B Tubulin (bovine brain, >99% pure, lyophilized) 1 x 1 mg $120.00 ¥1,680.00
TL238-C Tubulin (bovine brain, >99% pure, lyophilized) 5 x 1 mg $525.00 ¥7,350.00
TL238-D Tubulin (bovine brain, >99% pure, lyophilized) 10 x 1 mg $950.00 ¥13,300.00
TL238-DX Tubulin (bovine brain, >99% pure, lyophilized) 1 x 10 mg $875.00 ¥12,250.00
TL238-E Tubulin (bovine brain, >99% pure, lyophilized) 20 x 1 mg $1,780.00 ¥24,920.00
TL238-F Tubulin (bovine brain, >99% pure, lyophilized) Large quantities Inquire #VALUE!
TL440M-A Tubulin (AMCA dye labeled; porcine, micro-injctn) 5 x 20 µg $275.00 ¥3,850.00
TL440M-B Tubulin (AMCA dye labeled; porcine, micro-injctn) 20 x 20 µg $735.00 ¥10,290.00
TL488M-A Tubulin (HiLyte 488TM dye labeled; porcine, micro-injctn) 5 x 20 µg $275.00 ¥3,850.00
TL488M-B Tubulin (HiLyte 488TM dye labeled; porcine, micro-injctn) 20 x 20 µg $735.00 ¥10,290.00
TL590M-A Tubulin (Rhodamine labeled; porcine, micro-injctn) 5 x 20 µg $275.00 ¥3,850.00
TL590M-B Tubulin (Rhodamine labeled; porcine, micro-injctn) 20 x 20 µg $735.00 ¥10,290.00
TL620M-A Tubulin (X-rhodamine labeled; Bovine Brain micr-injctn) 5 x 20 µg $275.00 ¥3,850.00
TL620M-B Tubulin (X-rhodamine labeled; Bovine Brain micr-injctn) 20 x 20 µg $735.00 ¥10,290.00
TL670M-A Tubulin (HiLyte 647TM dye labeled; porcine, micro-injctn) 5 x 20 µg $275.00 ¥3,850.00
TL670M-B Tubulin (HiLyte 647TM dye labeled; porcine, micro-injctn) 20 x 20 µg $735.00 ¥10,290.00
TP005 Tubulin protein Soybean plant 1 x 250 µg $650.00 ¥9,100.00
TXD01 Paclitaxel  (2mM in DMSO) 10 x 100 µl $160.00 ¥2,240.00
V01-A Vimentin (Recombinant Syrian Hamster) 2 x 50 µg $295.00 ¥4,130.00
V01-C Vimentin (Recombinant Syrian Hamster) 10 x 50 µg $1,060.00 ¥14,840.00
VCG03-A VCA domain of WASP (GST fusion) 1 x 500 µg $375.00 ¥5,250.00
VCG03-B VCA domain of WASP (GST fusion) 5 x 500 µg $1,280.00 ¥17,920.00
 

k-assay KT-7519说明书

k-assay KT-7519说明书

Human Apoptosis InducingFactor (AIF) ELISA

诱导人凋亡
因子(AIF)ELISA
用于定量测定血清、血浆中的人类AIF,
组织匀浆和其他生物液体
货号:KT-7519

预期用途
该试剂盒是一种夹心酶免疫分析法,用于体外定量测定人体AIF
血清、血浆、组织匀浆和其他生物液体。仅供研究使用。不适用于
诊断程序。

需要但未提供的材料
1.带450±10 nm滤光片的微孔板阅读器。
2.单通道或多通道移液管,具有高精度和一次性j端。
3.微量离心管。
4.去离子水或蒸馏水。
5.用于吸干微孔板的吸水纸。
6.洗涤液容器。
7.0.01 mol/L(或1×)磷酸盐缓冲盐水(PBS),pH值7.0-7.2。
存储
所有试剂应按照瓶上的标签保存。校准器、检测试剂A、,
检测试剂B和96孔条形板在接收时应储存在-20°C下,而其他试剂则应储存在-20°C下
温度应为4°C。未使用的板条应保存在密封袋中,并向其提供干燥剂
尽量减少暴露在潮湿空气中。打开的测试套件将在1个月内保持稳定,前提是将其储存为
如上所述。

测试原理
本试剂盒中提供的微孔板已预涂有AIF特异性抗体。校准器
然后,将样品添加到适当的微孔板孔中,并加入一种特定于
AIF。接下来,将结合辣根过氧化物酶(HRP)的抗生物素蛋白添加到每个微孔板中,并
孵化。加入TMB底物溶液后,只有那些含有AIF、生物素结合抗体和酶结合亲和素的孔才会出现颜色变化。酶底物反应为
通过添加硫酸溶液终止,并测量颜色变化
在450 nm±10 nm的波长下进行分光光度测定。样品中的AIF浓度为
然后通过将样品的外径与校准曲线进行比较来确定。

样本收集和储存
血清
使用血清分离管,让样本在室温下凝结两小时,或在室温下凝结过夜
在大约1000 x g的条件下离心20分钟前,温度为4℃。测定新鲜制备的血清
立即或在-20°C或-80°C下等分储存样品,以备日后使用。避免反复冻融
周期。
等离子体
使用EDTA或肝素作为抗凝剂收集血浆。在1000 x的温度下离心样品15分钟
g在收集后30分钟内,在4°C下。立即清除血浆并进行分析,或将样品储存在
在-20°C或-80°C下等分供日后使用。避免重复冻融循环。
组织匀浆
组织匀浆的制备取决于组织类型。
1.在冰冷的PBS中冲洗组织,*清除多余的血液,并称重
均质化。
2.将组织切碎成小块,并在新鲜的溶解缓冲液(不同的溶解缓冲液)中均质
需要根据目标蛋白质的亚细胞位置进行选择(w:v=1:20-1:50,例如1毫升裂解
使用冰上玻璃均质器(微型组织研磨机)将缓冲液添加到20-50 mg组织样品中
也很有效)。
3.使用超声波细胞破碎机对所得悬浮液进行超声波处理,直到溶液澄清。
4.然后,匀浆在10000×g下离心5分钟。收集上清液并
立即测定或等分并储存在≤-20摄氏度。
其他生物液体
以1000 x g离心样品20分钟。收集上清液并立即进行分析或储存
在-20°C或-80°C下等分取样,以备日后使用。避免重复冻融循环。
注:
1.5天内使用的样品可储存在4°C下,否则样品必须储存在-20°C下
(≤1个月)或-80°C(≤2个月)以避免生物活性丧失和污染。
2.样本溶血会影响结果,因此不应使用溶血样本。
3.进行分析时,将样品置于室温。
4.根据我们的检测结果,强烈建议使用血清而不是血浆进行检测
内部数据

试剂制备
使用前,将所有套件组件和样品置于室温(18-25℃)。如果不使用该套件
一次完成后,请只取出当前实验的试纸和试剂,剩下的留着
所需条件下的试纸条和试剂。
校准器
用1.0 mL校准液稀释校准液,在室温下保存10分钟,
轻轻摇动(不要起泡)。储备溶液中校准器的浓度为4000 pg/mL。
请先将储备液稀释至1000pg/mL,稀释后的校准液为最高值
校准器(1000 pg/mL)。然后准备7根含有0.5 mL校准液稀释液的试管,并使用稀释液
根据下图所示,校准器产生双稀释系列。混合每根管子
在下一次转移前*检查。设置7点稀释校准器,如1000pg/mL、500pg/mL、,
250 pg/mL、125 pg/mL、62.5 pg/mL、31.2 pg/mL、15.6 pg/mL,以及最后一支带有校准器的EP管
稀释剂为空白,为0 pg/ml。

k-assay试剂盒产品目录

k-assay对于 ELISA 试剂盒,检测形式和检测范围可能会发生变化。


k-assay试剂盒产品目录

Research Assay Kits (Human) – Apoptosis
Product Description Photo Quantity Price* Cat. No.
Tumor Necrosis Factor Receptor Superfamily, Member 10C (TNFRSF10C), Human For quantitative detection in human samples.  (no photo available) 96 tests $952 KT-37819
Acidic mammalian chitinase (CHIA) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL005348HU
ADAMTS-like protein 4 (ADAMTSL4) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL001320HU
Amyloid beta A4 protein ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E14352h
Amyloid-like protein 1 (APLP1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL001911HU
Apoptosis Inducing Factor (AIF) ELISA Kit, Human  For quantitative detection in human samples.  (no photo available) 96 tests $952 KT-7519
Apoptosis inducing factor, AIF ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E09006h
Apoptosis inhibitor 4 (API4) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-EL002706HU
Apoptosis Inhibitor 5 (API5), Human For quantitative detection in human samples.  (no photo available) 96 tests $752 KT-37278
Apoptosis protease activating factor-1, Apaf-1 ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E08996h
Apoptosis regulator BAX ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $714 CSB-E09344h
Apoptosis Signal Regulating Kinase 1 (ASK1) ELISA Kit, Human  For quantitative detection in human samples.  (no photo available) 96 tests $952 KT-7529
Apoptosis-associated speck-like protein containing a CARD (PYCARD) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL019114HU
Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit, Human  For quantitative detection in human samples.  (no photo available) 96 tests $752 KT-7539
B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $705 KT-33787
B-cell leukemia/lymphoma 2, Bcl-2 ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E08853h
Baculoviral IAP repeat-containing protein 2 (BIRC2) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL002703HU
Baculoviral IAP repeat-containing protein 3 (BIRC3) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL002704HU
BAG family molecular chaperone regulator 1 (BAG1) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $975 KT-62273
BAG family molecular chaperone regulator 2 (BAG2) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $975 KT-62274
BAG family molecular chaperone regulator 3 (BAG3) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $975 KT-62275
BAG family molecular chaperone regulator 4 (BAG4) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $975 KT-62276
BAG family molecular chaperone regulator 5 (BAG5) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $975 KT-62277
Bcl-2-like protein 1 (BCL2L1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL002613HU
Bcl-2-like protein 11 (BCL2L11) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL002615HU
Bcl-2-related protein A1 (BCL2A1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL002612HU
Bcl2 antagonist of cell death (BAD) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL002528HU
Bcl2 Antagonist/Killer 1 (BAK1) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $757 KT-34045
Bcl2 Associated Athanogene 3 (BAG3) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $757 KT-34047
Bcl2 Associated Death Promoter (BAD) ELISA, Human, Human  For quantitative detection in human samples.  (no photo available) 96 tests $952 KT-38699
Bcl2 Associated X Protein (Bax) ELISA Kit, Human  For quantitative detection in human samples.  (no photo available) 96 tests $752 KT-8234
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL002767HU
BH3 Interacting Domain Death Agonist (Bid) ELISA Kit, Human  For quantitative detection in human samples.  (no photo available) 96 tests $752 KT-8351
BH3 interacting domain death agonist, Bid ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E08865h
Bifunctional apoptosis regulator (BFAR) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL002678HU
Cartilage oligomeric protein, COMP ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E09138h
Caspase 1 (Casp-1) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E13025h
Caspase 1 (CASP1) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $931 KT-33900
Caspase 12 (CASP12), Human For quantitative detection in human samples.  (no photo available) 96 tests $952 KT-37329
Caspase 14 (CASP14) ELISA Kit, Human  For quantitative detection in human samples.  (no photo available) 96 tests $752 KT-9404
Caspase 3 (CASP3) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $705 KT-33768
Caspase 3 ELISA For quantitative detection of human Caspase 3 in cell culture supernates, tissue homogenates, and serum. Assay Range: 31.2 – 2,000 pg/mL.  (no photo available) 1 kit $440 KT-1162
Caspase 4 (CASP4) ELISA Kit, Human  For quantitative detection in human samples.  (no photo available) 96 tests $752 KT-9434
Caspase 5 (CASP5) ELISA Kit, Human  For quantitative detection in human samples.  (no photo available) 96 tests $952 KT-9444
Caspase 6 (CASP6) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $957 KT-34048
Caspase 7 (CASP7) ELISA Kit, Human  For quantitative detection in human samples.  (no photo available) 96 tests $752 KT-9464
Caspase 8 (CASP8) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $652 KT-33805
Caspase 8 ELISA For quantitative detection of human Caspase 8 in cell culture supernates, tissue homogenates, and serum. Assay Range: 31.2 – 2,000 pg/mL.  (no photo available) 1 kit $467 KT-1163
Caspase 9 (CASP9) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $905 KT-33769
Caspase-6 (CASP6) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL004551HU
Caspase-7 (CASP7) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL004552HU
Caspase-9, Casp-9 ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $714 CSB-E08862h
Caspase-Activated DNase (CAD) ELISA Kit, Human  For quantitative detection in human samples.  (no photo available) 96 tests $952 KT-9494
CD40/TNFRSF5 ELISA For quantitative detection of human CD40 in cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine, and human milk. Assay Range: 15.6 – 1,000 pg/mL.  (no photo available) 1 kit $490 KT-1201
Cell adhesion molecule 1 (CADM1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL004425HU
Cell death activator CIDE-3 (CIDEC) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL005433HU
Clusterin, CLU ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E09121h
Cyclin Dependent Kinase 5, CDK5 ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E10102h
Cytochrome C ELISA  For the quantitative determination of human cytochrome C in cell culture supernatant, whole blood, and serum. Contains cytochrome C standard. Assay Range: 0 – 5 ng/mL. Sensitivity: 0.08 ng/mL.   
  
Click Image To Enlarge  

96 tests $783 KT-002
Cytochrome-C, Cyt-C ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E08530h
Cytokeratin 20, CK20 ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E11710h
DCR3/TNFRSF6B ELISA For quantitative detection of human DCR3 in cell culture supernates, serum, and plasma (heparin, EDTA). Assay Range: 156 – 10,000 pg/mL.  (no photo available) 1 kit $467 KT-1241
Death Associated Protein Kinase 1 (DAPK1) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $757 KT-34074
Death domain-associated protein 6 (DAXX) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL006507HU
Death Inducer Obliterator 1 (DIDO1) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $957 KT-34179
Death receptor 5 (DR5), Human For quantitative detection in human samples.  (no photo available) 96 tests $952 KT-37409
Dedicator of cytokinesis protein 1 (DOCK1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL007094HU
Deoxyribonuclease gamma (DNASE1L3) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL007052HU
Deoxyribonuclease I, DNase-I ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E09068h
Diablo/SMAC ELISA For quantitative detection of human Diablo in cell culture supernates, serum, and plasma (heparin, EDTA). Assay Range: 31.2 – 2,000 pg/mL.  (no photo available) 1 kit $467 KT-1243
Dipeptidyl Peptidase 7 (DPP7), Human  For quantitative detection in human samples.  (no photo available) 96 tests $752 KT-38666
DNA damage-inducible transcript 4 protein (DDIT4) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL006590HU
Dual specificity mitogen-activated protein kinase kinase 4 (MAP2K4) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL013413HU
Extracellular sulfatase Sulf-1 (SULF1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL022930HU
Factor Related Apoptosis (FAS) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $952 KT-14443
Factor-related Apoptosis, FAS ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $474 CSB-E04542h
Fas apoptotic inhibitory molecule 1 (FAIM) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL007970HU
FASL ELISA For quantitative detection of human FASL in cell culture supernates, serum, and plasma (heparin, EDTA, citrate). Assay Range: 15.6 – 1,000 pg/mL.  (no photo available) 1 kit $505 KT-1262
Fibroblast growth factor receptor 2 (FGFR2) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL008645HU
Fibroblast growth factor receptor 3 (FGFR3) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL008646HU
Forkhead box O3, FOXO3 ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E11177h
Forkhead Box Protein P1 (FOXP1), Human For quantitative detection in human samples.  (no photo available) 96 tests $952 KT-37469
G protein-coupled receptor kinase 5 (GRK5) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL009926HU
GLP-1 ELISA, Human, Mouse, Rat  For the quantitative determination of Glucagon-Like Peptide-1 in rat, mouse, and human plasma. Assay range: 0.206 – 50 ng/mL.   
  
Click Image To Enlarge  

96 tests $998 KT-384
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH/G3PDH) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E13911h
Granzyme A ELISA For quantitative detection of human Granzyme A in cell culture supernates, serum, and plasma (heparin, EDTA). Assay Range: 15.6 – 1,000 pg/mL.  (no photo available) 1 kit $467 KT-1292
Guanine nucleotide-binding protein subunit beta-2-like 1 (GNB2L1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL009605HU
HLA-B associated transcript 3 (BAT3) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL002567HU
Homeodomain-interacting protein kinase 2 (HIPK2) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL010368HU
Huntingtin (HTT) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL010905HU
Interferon alpha-inducible protein 27, mitochondrial (IFI27) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL011009HU
Interleukin 19 (IL-19) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E17323h
Interleukin 24 (IL-24) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E15840h
Livin ELISA For quantitative detection of human Livin in cell culture supernates, serum, cell lysates, and tissue homogenates. Assay Range: 0.78 – 50 ng/mL.  (no photo available) 1 kit $542 KT-1371
MAP kinase-activating death domain protein (MADD) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL013309HU
Maternal embryonic leucine zipper kinase (MELK) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL013692HU
Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL013403HU
Mothers Against Decapentaplegic Homolog 2 (Smad2), Human, Mouse, Rat For quantitative detection in human, mouse, rat samples.  (no photo available) 96 tests $752 KT-37868
NACHT, LRR and PYD domains-containing protein 1 (NLRP1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL015864HU
NACHT, LRR and PYD domains-containing protein 3 (NLRP3/C1orf7/CIAS1/NALP3/PYPAF1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E15885h
NAD-dependent deacetylase sirtuin-1 (SIRT1/SIR2L1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E15058h
Netrin receptor DCC (DCC) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL006536HU
Netrin receptor UNC5B (UNC5B) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL025629HU
Netrin receptor UNC5D (UNC5D) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL025632HU
Netrin-1, Ntn1 ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E11899h
Neutrophil gelatinase-associated lipocalin, NGAL ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $700 KU-181
NLR family CARD domain-containing protein 4 (NLRC4) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL015862HU
Nuclear factor-kappa B, NF-kappaB ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E12107h
Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL015914HU
OPG ELISA For quantitative detection of human OPG in cell culture supernates, serum, plasma (heparin, EDTA), saliva, and urine. Assay Range: 93.7 – 6,000 pg/mL.  (no photo available) 1 kit $463 KT-1408
P2X purinoceptor 1 (P2RX1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL017319HU
P53 ELISA For quantitative detection of human P53 in cell lysates. Assay Range: 156 – 10,000 pg/mL.  (no photo available) 1 kit $505 KT-1411
PD-1 ELISA For quantitative detection of human PD-1 in cell culture supernates. Assay Range: 156 – 10,000 pg/mL.  (no photo available) 1 kit $489 KT-1415
Phosphatidylcholine:ceramide cholinephosphotransferase 1 (SGMS1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL021193HU
Pleckstrin homology-like domain family A member 3 (PHLDA3) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL017932HU
Presenilin 1 (PS-1) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E17832h
PRKC apoptosis WT1 regulator protein (PAWR) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL017486HU
Programmed cell death 4 (PDCD4) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E13638h
Programmed cell death 6-interacting protein (PDCD6IP) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL017673HU
Programmed Cell Death Protein 1 (PDCD1) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $905 KT-33784
Programmed cell death protein 5 (PDCD5) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL017671HU
Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $731 KT-33855
Prostaglandin E Synthase, Microsomal (PTGES) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $752 KT-26231
Proteasome activator complex subunit 3 (PSME3) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL018918HU
Protein DJ-1 (PARK7) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E12024h
Protein FADD (FADD) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL007958HU
Protein FAM3B (FAM3B) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL008227HU
Protein JTB (JTB) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL011970HU
Protein SCAF11 (SCAF11), Human For quantitative detection in human samples.  (no photo available) 96 tests $752 KT-37694
Proto-oncogene serine/threonine-protein kinase pim-1 (PIM1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E11825h
PTB domain-containing engulfment adapter protein 1 (GULP1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL010062HU
RAC-alpha serine/threonine-protein kinase (AKT1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL001553HU
Receptor-binding cancer antigen expressed on SiSo cells, RCAS1 ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E12153h
Retrotransposon-derived protein PEG10 (PEG10) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL017772HU
Ribosomal protein S6 kinase beta-1 (RPS6KB1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL020470HU
sCD40 ELISA, Human  For the quantitative detection of human soluble CD40 in cell culture supernatant, plasma, amniotic fluid, and serum. Contains standard. Assay Range: 0 – 500 pg/mL. Sensitivity: 12 pg/mL.  (no photo available) 96 tests $783 KT-003
sCD40L ELISA For quantitative detection of human soluble CD40L in cell culture supernates, serum, and plasma (heparin, EDTA). Assay Range: 62.5 – 4,000 pg/mL.  (no photo available) 1 kit $514 KT-1441
sCD40L ELISA, Human  For the quantitative detection of human soluble CD40L in cell culture supernatant and serum. Contains standard. Assay Range: 0 – 10 ng/mL. Sensitivity: 0.095 ng/mL.  (no photo available) 96 tests $783 KT-004
Serglycin (SRGN) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL022664HU
Serine/threonine-protein kinase 24 (STK24) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E13532h
Serine/threonine-protein kinase PAK 2 (PAK2) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL017406HU
Serine/threonine-protein kinase Sgk1 (SGK1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL021189HU
Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL007795HU
Serine/threonine-protein kinase/endoribonuclease IRE2 (ERN2) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL007796HU
sFAS ELISA For quantitative detection of human FAS in cell culture supernates, serum, and plasma (heparin, EDTA, citrate). Assay Range: 31.2 – 2,000 pg/mL.  (no photo available) 1 kit $463 KT-1448
sFas Ligand ELISA  For the quantitative detection of human soluble Fas Ligand in cell culture supernatant, plasma, urine, amniotic fluid, and serum. Contains standard. Assay Range: 0 – 10 ng/mL. Sensitivity: 0.1 ng/ mL.  (no photo available) 96 tests $739 KT-001
Signal Transducer And Activator Of Transcription 3 (STAT3), Human, Mouse, Rat For quantitative detection in human, mouse, rat samples.  (no photo available) 96 tests $752 KT-37878
Soluble tumor necrosis factor receptor 2, sTNF-R2 ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E11266h
Soluble tumor necrosis factor-related apoptosis inducing ligand, sTRAIL ELISA KIT, Human For quantitative detection in human samples.  (no photo available) 96 tests $714 CSB-E04750h
Tensin-4 (TNS4) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL024034HU
TGF Beta Inducible Early Response Gene 1 (TIEG1) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $752 KT-29598
TGF-beta receptor type-1 (TGFBR1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL023451HU
TGFB1-induced anti-apoptotic factor 1 (TIAF1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL023528HU
TNFRSF9/4-1BB ELISA  For quantitative detection of human TNFRSF9 in cell culture supernates, serum, and plasma ( heparin, EDTA). Assay Range: 31.2 – 2,000 pg/mL.  (no photo available) 1 kit $467 KT-1499
TNFsR I ELISA  For quantitative detection of human TNFsR?in cell culture supernates, serum, plasma ( heparin, EDTA, citrate), and urine. Assay Range: 7.8 – 500 pg/mL.  (no photo available) 1 kit $490 KT-1505
TRAIL ELISA For quantitative detection of human TRAIL in cell culture supernates, cell lysates, serum, plasma (heparin, EDTA), and saliva. Assay Range: 15.6 – 1,000 pg/mL.  (no photo available) 1 kit $490 KT-1506
Transcription Factor P65 (REL-A) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $752 KT-30240
Transforming growth factor beta receptor 2 (TGF-betaR2) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-E13669h
Tribbles homolog 3 (TRIB3) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL024446HU
Trichoplein keratin filament-binding protein (TCHP) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL023311HU
Tumor necrosis factor alpha-induced protein 3 (TNFAIP3) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL023958HU
Tumor necrosis factor receptor superfamily member 19 (TNFRSF19) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL023976HU
Tumor necrosis factor receptor superfamily member 21 (TNFRSF21) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL023979HU
Tumor necrosis factor receptor superfamily member 25 (TNFRSF25) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL023980HU
Tumor necrosis factor receptor superfamily member 3 (LTBR) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL013227HU
Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) ELISA, Human, Human  For quantitative detection in human samples.  (no photo available) 96 tests $952 KT-38683
Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $868 KT-32108
Tumor necrosis factor soluble receptor I, TNFsR-IELISA KIT, Human For quantitative detection in human samples.  (no photo available) 96 tests $650 KU-018
Tumor necrosis factor-associated factor 1, TRAF1 ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $948 CSB-E13601h
Tumor necrosis factor-related apoptosis-inducing ligand receptor 1 (TRAIL-R1) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $714 CSB-E04747h
Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2 (TRAIL-R2/DR5) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $714 CSB-E13164h
Tumor necrosis factor-related apoptosis-inducing ligand receptor 4 (TRAIL-R4) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $714 CSB-E04749h
Tyrosine-protein kinase ABL1 (ABL1) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL001105HU
Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL002294HU
Ubiquitin-like protein ATG12 (ATG12) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL002283HU
Ubiquitin-like-conjugating enzyme ATG3 (ATG3) ELISA kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $834 CSB-EL002288HU
X-Linked Inhibitor Of Apoptosis Protein (XIAP) ELISA Kit, Human For quantitative detection in human samples.  (no photo available) 96 tests $757 KT-34122

 

S100B ELISA Assay Kit 说明书

世界*实验材料供应商Eagle Biosciences 上海金畔生物为其中国代理, Eagle Biosciences 在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务,Eagle Biosciences 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

 Eagle Biosciences 中国代理, Eagle Biosciences上海代理, Eagle Biosciences北京代理,Eagle Biosciences广东代理, Eagle Biosciences江苏代理Eagle Biosciences湖北代理, Eagle Biosciences 天津,Eagle Biosciences 黑龙江代理, Eagle Biosciences 内蒙古代理, Eagle Biosciences 吉林代理, Eagle Biosciences 福建代理, Eagle Biosciences 江苏代理, Eagle Biosciences 浙江代理, Eagle Biosciences 四川代理,

 

Eagle Biosciences已迅速成为一个的抗体,免疫分析,蛋白质的研究和临床社区。我们的员工在研究和诊断行业有超过25年的时间,超过10年的商业化和销售。我们提供eaglebio开发的产品除了销售的产品来自二十多个欧洲组织。在所有这些产品中,我们主要致力于提供帮助医学研究和临床实验室的深奥创新产品。

 

S100B ELISA Assay Kit

货号: DCM074

产品描述

S100B ELISA测定试剂盒: 
仅供研究使用
尺寸:1 x 96
孔灵敏度:35.37 pg / ml
动态范围:35.37 – 5000 pg / ml
孵育时间:3.5小时
样品类型:血清,血浆
样品量:50μl
对照

有可能的使用

Eagle Biosciences S100B ELISA测定试剂盒是用于定量测定人血清或血浆中S100B浓度的免疫酶学比色法。S100B ELISA测定试剂盒仅供研究使用。

测定原理

S100B ELISA测定试剂盒基于S100B通过两种抗体的结合,一种固定在微孔板上,另一种与辣根过氧化物酶(HRP)结合。该测定是两步结合方法,并且在每个孵育步骤之后,通过简单的固相洗涤进行结合/自由分离。然后,结合级分中的酶HRP与底物(H 2 O 2)和TMB底物反应,并且当添加停止溶液(H 2 SO 4)时,发展出变成黄色的蓝色。颜色强度与样品中的S100B浓度成比例。通过校准曲线计算样品中的S100B浓度。

测定背景

S100是属于EF-手钙结合蛋白的S100 /钙调素/肌钙蛋白C超家族的20kDa蛋白质。S100zui初是从人脑分离出来的,并被认为是胶质细胞特异性蛋白质(1)。今天,已经根据结构和功能相似性确定了S100家族的20种单体(2,3)。大多数S100蛋白以二聚体形式存在并以细胞特异性方式表达。被称为S100α和S100β(4)的S100单体中的两种在物种之间是高度保守的,并且在中枢神经系统胶质细胞和某些外周细胞中被发现为同型(ββ)和异二聚体(αβ)。雪旺氏细胞,黑素细胞,脂肪细胞和软骨细胞(5)。S100αβ和S100ββ也存在于恶性组织中,zui显着的是黑素瘤,而在胶质瘤,甲状腺细胞癌和肾细胞癌中也较少。血清中S100B(如S100αβ和S100ββ单位)的测定已被证明在诊断为恶性黑素瘤的患者的预后和治疗监测中具有临床意义(6-9)。研究还表明,S100B可能有助于治疗患有脑损伤的患者。创伤性头部损伤,围产期窒息,心脏骤停,心脏手术和中风(10-13)。

 

Assay Biotechnology B0020 说明书

世界*实验材料供应商 Assay Biotechnology上海金畔生物为其中国代理, Assay Biotechnology在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Assay Biotechnology就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Assay Biotechnology中国代理Assay Biotechnology上海代理, Assay Biotechnology北京代理,Assay Biotechnology广东代理, Assay Biotechnology江苏代理Assay Biotechnology湖北代理,Assay Biotechnology天津,Assay Biotechnology黑龙江代理,Assay Biotechnology内蒙古代理,Assay Biotechnology吉林代理,Assay Biotechnology福建代理, Assay Biotechnology江苏代理, Assay Biotechnology浙江代理, Assay Biotechnology四川代理,

 

自2006年1月成立以来,Assay Biotechnology一直是业界的抗体生产和工程和测定技术,荧光染料,猝灭剂,重组蛋白和合成肽的贡献者。位于加利福尼亚州旧金山湾区的心脏地带,我们一直努力应对高质量检测试剂盒和免疫学试剂的需求,用于制药和学术研究,主要制定医疗保健基础。
在我们成立后的头几年,Assay Biotech成立了抗体制造商和抗体工程公司,通过OEM合作伙伴关系专门针对大型公司。从那时起,我们一直在成为一个突出的测定和抗体来源,将其覆盖范围扩大到超过50个国家的数百万研究人员。
我们一直在寻求推动我们潜力的信条,决心成为*的测定开发和制造公司之一,提供大量即用型测定,免疫试剂,分子检测模块,生物标志物和全线抗体工程服务支持zui艰巨的学术,药学和临床研究工作。

 

MYC ANTIBODY

Catalog No Size  
B0020                         50ul    100ul                       
Reactivity Applications MW Source
H, M, R WB IHC IF ELISA 48 kDa Rabbit
Product Name MYC Antibody
Synonyms Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64 ; MYC ; BHLHE39
Immunogen The antiserum was produced against synthesized peptide derived from human MYC.
Immunogen Range 31-80
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity MYC Antibody detects endogenous levels of total MYC protein.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol MYC
Database Links

Gene ID: 4609 Swiss-Prot #: P01106

OMIM #: 113970/190080 Unigene #: Hs.202453

Target Modification Unmodified/Total
Modification Sites  
Application Images
Image 1 Immunofluorescence analysis of HeLa cells, using MYC Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MYC Antibody. The picture on the right is blocked with the synthesized peptide.
Image 3 Western blot analysis of lysates from HeLa cells, treated with Forskolin 40nM 30', using MYC Antibody. The lane on the right is blocked with the synthesized peptide.

 

 

 

 

Assay Biotechnology A7159 说明书

世界*实验材料供应商 Assay Biotechnology上海金畔生物为其中国代理, Assay Biotechnology在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Assay Biotechnology就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Assay Biotechnology中国代理Assay Biotechnology上海代理, Assay Biotechnology北京代理,Assay Biotechnology广东代理, Assay Biotechnology江苏代理Assay Biotechnology湖北代理,Assay Biotechnology天津,Assay Biotechnology黑龙江代理,Assay Biotechnology内蒙古代理,Assay Biotechnology吉林代理,Assay Biotechnology福建代理, Assay Biotechnology江苏代理, Assay Biotechnology浙江代理, Assay Biotechnology四川代理,

 

自2006年1月成立以来,Assay Biotechnology一直是业界的抗体生产和工程和测定技术,荧光染料,猝灭剂,重组蛋白和合成肽的贡献者。位于加利福尼亚州旧金山湾区的心脏地带,我们一直努力应对高质量检测试剂盒和免疫学试剂的需求,用于制药和学术研究,主要制定医疗保健基础。
在我们成立后的头几年,Assay Biotech成立了抗体制造商和抗体工程公司,通过OEM合作伙伴关系专门针对大型公司。从那时起,我们一直在成为一个突出的测定和抗体来源,将其覆盖范围扩大到超过50个国家的数百万研究人员。
我们一直在寻求推动我们潜力的信条,决心成为*的测定开发和制造公司之一,提供大量即用型测定,免疫试剂,分子检测模块,生物标志物和全线抗体工程服务支持zui艰巨的学术,药学和临床研究工作。

 

MYC (PHOSPHO-THR58) ANTIBODY

Catalog No Size  
A7159                         50ul    100ul                         
Reactivity Applications MW Source
H, M, R WB IHC IP ELISA 48 kDa Rabbit
Product Name Myc (Phospho-Thr58) Antibody
Synonyms Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64 ; MYC ; BHLHE39
Immunogen The antiserum was produced against synthesized peptide derived from human Myc around the phosphorylation site of Thr58.
Immunogen Range 25-74
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity Myc (Phospho-Thr58) Antibody detects endogenous levels of Myc only when phosphorylated at Thr58.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol MYC
Database Links

Gene ID: 9531 Swiss-Prot #: P01106

OMIM #: 190080 Unigene #: Hs.202453

Target Modification Phospho
Modification Sites H:T58 M:T58 R:T58
Application Images
Image 1 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Thr58) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Western blot analysis of lysates from ovary cancer, using Myc (Phospho-Thr58) Antibody. The lane on the right is blocked with the phospho peptide.

 

Assay Biotechnology A7118 说明书

世界*实验材料供应商 Assay Biotechnology上海金畔生物为其中国代理, Assay Biotechnology在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Assay Biotechnology就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Assay Biotechnology中国代理Assay Biotechnology上海代理, Assay Biotechnology北京代理,Assay Biotechnology广东代理, Assay Biotechnology江苏代理Assay Biotechnology湖北代理,Assay Biotechnology天津,Assay Biotechnology黑龙江代理,Assay Biotechnology内蒙古代理,Assay Biotechnology吉林代理,Assay Biotechnology福建代理, Assay Biotechnology江苏代理, Assay Biotechnology浙江代理, Assay Biotechnology四川代理,

 

自2006年1月成立以来,Assay Biotechnology一直是业界的抗体生产和工程和测定技术,荧光染料,猝灭剂,重组蛋白和合成肽的贡献者。位于加利福尼亚州旧金山湾区的心脏地带,我们一直努力应对高质量检测试剂盒和免疫学试剂的需求,用于制药和学术研究,主要制定医疗保健基础。
在我们成立后的头几年,Assay Biotech成立了抗体制造商和抗体工程公司,通过OEM合作伙伴关系专门针对大型公司。从那时起,我们一直在成为一个突出的测定和抗体来源,将其覆盖范围扩大到超过50个国家的数百万研究人员。
我们一直在寻求推动我们潜力的信条,决心成为*的测定开发和制造公司之一,提供大量即用型测定,免疫试剂,分子检测模块,生物标志物和全线抗体工程服务支持zui艰巨的学术,药学和临床研究工作。

 

INTEGRIN BETA3 (PHOSPHO-TYR773) ANTIBODY

Catalog No Size  
A7118                         50ul    100ul                       

 

Product Name Integrin beta3 (Phospho-Tyr773) Antibody
Synonyms CD61; GP3A; GPIIIa; integrin beta-3; ITB3; plaet membrane glycoprotein IIIa
Immunogen The antiserum was produced against synthesized peptide derived from human Integrin beta3 around the phosphorylation site of Tyr773.
Immunogen Range 739-788
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity Integrin beta3 (Phospho-Tyr773) Antibody detects endogenous levels of Integrin beta3 only when phosphorylated at Tyr773.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol ITGB3
Database Links

Gene ID: 3690 Swiss-Prot #: P05106

OMIM #: 173470/273800 Unigene #: Hs.218040

Target Modification Phospho
Modification Sites H:Y773 M:Y772 R:Y772
Application Images
Image 1 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Integrin beta3 (Phospho-Tyr773) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Western blot analysis of lysates from HL-60 cells treated with H2O2, using Integrin beta3 (Phospho-Tyr773) Antibody. The lane on the right is blocked with the phospho peptide.