Alexa Fluor 647抗SAM 1a/b说明书

 

arthusbio Alexa Fluor 647抗SAM 1a/b说明书

 

Alexa Fluor 647-anti-SAM 1a/b

产品名称
Alexa Fluor 647抗SAM 1a/b
目录号MAF00201-25/50
描述
Alexa Fluor9647(AF647)共轭抗S腺苷甲硫氨酸单克隆抗体克隆118-6
特异性
MAF00201显示出与未结合的小鼠抗SAM单克隆抗体MA00201相同的特异性。

 

 

属性
类型
液体
存储说明
在2-8°C的黑暗中储存,不要冷冻。
浓度
2-4mg/ml,特定批次
存储缓冲区
50mM Tris,150mM NaCl,pH8.0,0.2%BSA(Sigma),0.09%NaN3
稀释液
PBS,pH 7.4,1%胎牛血清或0.5%BSA,0.09%NaN3
纯洁
>95%用Sephacryl S-200纯化,不含未结合抗体和Alexa FluorP 647
克隆性
单克隆抗体
克隆编号
118-6
免疫球蛋白同种型小鼠lgG2b
研究领域
生物分子(DNA、RNA、蛋白质、激素、神经递质等)的甲基化
单碳代谢
信号转导
新陈代谢
路径和过程
巨蟹座
关节炎
神经变性疾病
动脉粥样硬化
肝脏疾病
肾脏疾病

 

应用
已经测试了MAF00201在以下应用中的使用。应用说明包括推荐和测试的稀释液。最佳稀释度/浓度应由最终用户根据测试环境和目的确定。

 

图1用40μg/ml的AF488抗SAH 301-3(Cat#MAF00301)和8μg/ml的AF647抗SAM 118-6(Cat#MAF00201)对HepG2和L02细胞进行免疫荧光(IF)染色,然后进行DAPI染色,并在激光扫描共聚焦显微镜(蔡司LSM 780)下拍摄。(A1-A4)正常肝细胞系L02细胞在含有10%FBS的RPMI 1640中培养40h。
不同观点如下:DAPI(A1);AF488用于SAH(A2);SAM的AF647(A3);所有三个荧光信号的重叠(A4)。(B1-B4)肝细胞癌细胞系HepG2细胞培养40小时。不同观点如下:DAPI(B1);AF488用于SAH(B2);用于SAM(B3)的AF647;所有三个荧光信号的重叠(B4)。在L02和HepG2细胞(x630)之间,SAM和SAH的表达模式不同。

 

图2:36μg/ml下Alexa Fluor9488结合抗SAH抗体301-3(Cat#MAF00301)和4.5 ug/ml下Alexa Fluorm 647结合抗SAM抗体118-6(Cat#MAF00201)双重染色的L02(A1-A3)和HepG2(B1-B3)细胞的流式细胞术。
颜色图例:
橙色:空白;蓝色:使用核固定/通透性缓冲液(eBioscience 00-5523 FoxP3uTF染色缓冲液组);红色:使用细胞内固定/渗透缓冲液(eBioscience 00-8824)。用细胞内固定/通透性缓冲液(A2,B2)或核固定/通透性缓冲液(A3,B3)固定并通透性融合细胞(在含10%FBS的RPMI 1640中培养48小时),然后用上述抗体双重染色。使用BD-FACSCalibur流式细胞仪分析细胞。
SAM在L02细胞中的表达高于HepG2细胞。SAM和SAH都广泛表达,但相当动态。两种细胞中SAM的水平均高于SAH。

 

 

 

arthusbio MAF00201-25/50

 

Alexa Fluor 350标记山羊抗小鼠IgG(H+L)(A0412)

Alexa Fluor 350标记山羊抗小鼠IgG(H+L)
Alexa Fluor 350标记山羊抗小鼠IgG(H+L)(A0412) 一键复制产品信息

产品编号: A0412

产品包装:100μl
选择包装

100μl

说明书下载

528.00 510.00 10

价格: ¥ 528.00

促销价: ¥ 528.00

促销价: ¥ 528.00 510.00

产品简介
使用说明
产品文件
相关产品
相关论文
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产品编号 产品名称 产品包装 产品价格
A0412 Alexa Fluor 350标记山羊抗小鼠IgG(H+L) 100μl 528.00元

本Alexa Fluor 350标记山羊抗小鼠IgG(H+L)(Alexa Fluor 350-labeled Goat Anti-Mouse IgG(H+L))为进口分装,用于免疫荧光染色。
Alexa Fluor 350是一种常用的非常明亮的蓝色荧光探针。Alexa Fluor 350的荧光光谱与DAPI和Hoechst比较接近。 Alexa Fluor 350的吸收(激发)和发射峰参见下表。

Fluorophore Absorption Peak (nm) Emission Peak (nm)
Alexa Fluor 350 346 442

本抗体为用纯化的小鼠IgG免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人血清、人IgG、山羊IgG、牛IgG(bovine IgG)、兔IgG和大鼠IgG吸附纯化的优质二抗。对人血清、人IgG、山羊IgG、牛IgG(bovine IgG)、兔IgG和大鼠IgG几乎没有结合能力。特别适合于对于二抗种属特异性要求比较高的荧光染色实验。
本Alexa Fluor 350标记山羊抗小鼠IgG(H+L)用于免疫荧光染色时的推荐稀释比例为1:500。实际实验操作过程中需根据抗原和抗体的具体情况适当调节荧光标记二抗的稀释比例,推荐的调节范围为1:200-1000。
本抗体如果用于常规的免疫染色,以每次检测需1毫升1:500稀释的荧光标记二抗计,至少可以检测50次。如果适当重复使用已经使用过的荧光标记二抗,至少可以多检测150-250次。
包装清单:

产品编号 产品名称 包装
A0412 Alexa Fluor 350标记山羊抗小鼠IgG (H+L) 100μl
说明书 1份

保存条件:
-20℃避光保存,一年有效。
注意事项:
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1. 免疫荧光染色请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
2. 如果希望重复使用稀释的荧光标记二抗,稀释的荧光标记二抗4℃保存。 

Alexa Fluor 350标记山羊抗小鼠IgG(H+L)(A0412)
Alexa Fluor 350标记山羊抗小鼠IgG(H+L)(A0412)

物质安全数据单
输入右侧验证码点击下载:

Alexa Fluor 350标记山羊抗小鼠IgG(H+L)(A0412)

Alexa Fluor 350标记山羊抗小鼠IgG(H+L)(A0412)
质检证书
输入批号搜寻COA

搜索

相关产品请点击如下按钮:
荧光标记二抗

使用本产品的文献:

1. Shuangqi Fan, Keke Wu, Mingqiu Zhao, Jin Yuan, Shengming Ma, Erpeng Zhu, Yuming Chen, Hongxing Ding, Lin Yi, Jinding Chen
LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection
Autophagy. 2021 Sep;17(9):2305-2324.;doi: 10.1080/15548627.2020.1823123. (IF 9.77)

2. Shuangqi Fan, Keke Wu, Chaowei Luo, Xin Li, Mengpo Zhao, Dan Song, Shengming Ma, Erpeng Zhu, Yuming Chen, Hongxing Ding, Lin Yi, Jun Li, Mingqiu Zhao, Jinding Chen
Dual NDP52 Function in Persistent CSFV Infection
Front Microbiol. 2020 Jan 8;10:2962.;doi: 10.3389/fmicb.2019.02962. (IF 4.235)

3. Ke Yang, Lianghui Zhan, Tingting Lu, Cong Zhou, Xue Chen, Yingjie Dong, Guiyuan Lv, Suhong Chen
Dendrobium officinale polysaccharides protected against ethanol-induced acute liver injury in vivo and in vitro via the TLR4/NF-κB signaling pathway
Cytokine. 2020 Mar 26;130:155058.;doi: 10.1016/j.cyto.2020.155058. (IF 2.952)

4. Zhigang Yi, Yanchuan Pu, Ruoyan Gou, Yonggang Chen, Xiaojun Ren, Wenzhong Liu, Ping Dong
Silencing of RIPK4 inhibits epithelial‑mesenchymal transition by inactivating the Wnt/β‑catenin signaling pathway in osteosarcoma
Mol Med Rep. 2020 Mar;21(3):1154-1162.;doi: 10.3892/mmr.2020.10939. (IF 2.1)

5. Kai Zhang, Tianyuan Wang, Xingjing Liu, Qingzhao Yuan, Tin Xiao, Xiangjiang Yuan, Yijian Zhang, Li Yuan, Yao Wang
CASK, APBA1, and STXBP1 collaborate during insulin secretion
Mol Cell Endocrinol. 2021 Jan 15;520:111076.;doi: 10.1016/j.mce.2020.111076. (IF 3.871)

Alexa Fluor 488标记山羊抗兔IgG(H+L)(A0423)

Alexa Fluor 488标记山羊抗兔IgG(H+L)
Alexa Fluor 488标记山羊抗兔IgG(H+L)(A0423) 一键复制产品信息

产品编号: A0423

产品包装:100μl
选择包装

100μl

说明书下载

503.00 486.00 10

价格: ¥ 503.00

促销价: ¥ 503.00

促销价: ¥ 503.00 486.00

产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
A0423 Alexa Fluor 488标记山羊抗兔IgG(H+L) 100μl 503.00元

本Alexa Fluor 488标记山羊抗兔IgG(H+L)(Alexa Fluor 488-labeled Goat Anti-Rabbit IgG(H+L))为进口分装,用于免疫荧光染色。
488是一种常用的非常明亮的绿色荧光探针。它比绝大部分常用的绿色荧光探针更加明亮,更加不容易淬灭,而且背景更低。Alexa Fluor 488的荧光光谱与FITC和Cy2比较接近。Alexa Fluor 488的吸收(激发)和发射峰参见下表。

Fluorophore Absorption Peak (nm) Emission Peak (nm)
Alexa Fluor 488 495 519

本抗体为用纯化的兔IgG免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人IgG、小鼠IgG和大鼠IgG吸附纯化的优质二抗。对人IgG、小鼠IgG和大鼠IgG几乎没有结合能力。特别适合于对于二抗种属特异性要求比较高的荧光染色实验。
本Alexa Fluor 488标记山羊抗兔IgG(H+L)用于免疫荧光染色时的推荐稀释比例为1:500。实际实验操作过程中需根据抗原和抗体的具体情况适当调节荧光标记二抗的稀释比例,推荐的调节范围为1:200-1000。
本抗体如果用于常规的免疫染色,以每次检测需1毫升1:500稀释的荧光标记二抗计,至少可以检测50次。如果适当重复使用已经使用过的荧光标记二抗,至少可以多检测150-250次。
包装清单:

产品编号 产品名称 包装
A0423 Alexa Fluor 488标记山羊抗兔IgG (H+L) 100μl
说明书 1份

保存条件:
-20℃避光保存,一年有效。
注意事项:
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1.免疫荧光染色请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
2.如果希望重复使用稀释的荧光标记二抗,稀释的荧光标记二抗4℃保存。

Alexa Fluor 488标记山羊抗兔IgG(H+L)(A0423)
Alexa Fluor 488标记山羊抗兔IgG(H+L)(A0423)

物质安全数据单
输入右侧验证码点击下载:

Alexa Fluor 488标记山羊抗兔IgG(H+L)(A0423)

Alexa Fluor 488标记山羊抗兔IgG(H+L)(A0423)
质检证书
输入批号搜寻COA

搜索

相关产品请点击如下按钮:
荧光标记二抗

使用本产品的文献:

1. Su J, Wang Y, Xing X, Zhang L, Sun H, Zhang Y.
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Mol Cell Biochem. 2012 Jul;366(1-2):101-10. (IF 2.795)

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Regulation of SIV antigen-specific CD4+ T cellular immunity via autophagosome-mediated MHC II molecule-targeting antigen presentation in mice.
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Effects of 3-hydroxyflavone on the cellular and molecular characteristics of bovine embryos produced by somatic-cell nuclear transfer.
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Oocyte-secreted factors in oocyte maturation media enhance subsequent development of bovine cloned embryos.
Mol Reprod Dev. 2014 Apr;81(4):341-9. (IF 2.823)

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Reactive oxygen species and autophagy associated apoptosis and limitation of clonogenic survival induced by zoledronic acid in salivary adenoid cystic carcinoma cell line SACC-83.
PLoS One. 2014 Jun 25;9(6):e101207. (IF 2.74)

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Antialcoholic liver activity of whey fermented by Lactobacillus casei isolated from koumiss.
J Dairy Sci. 2014 Jul;97(7):4062-71. (IF 3.333)

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Astrocytic JWA Expression is Essential to Dopaminergic Neuron Survival in the Pathogenesis of Parkinson’s Disease.
CNS Neurosci Ther. 2014 Aug;20(8):754-62. (IF 4.074)

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Recombinant human growth differentiation factor-9 improves oocyte reprogramming competence and subsequent development of bovine cloned embryos.
Cell Reprogram. 2014 Aug;16(4):281-9. (IF 1.702)

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Toll-like receptor 3 inhibits Newcastle disease virus replication through activation of pro-inflammatory cytokines and the type-1 interferon pathway.
Arch Virol. 2014 Nov;159(11):2937-48. (IF 2.243)

37. Dong WL, Hou CC, Yang WX.
Mitochondrial prohibitin and its ubiquitination during crayfish Procambarus clarkii spermiogenesis.
Cell Tissue Res. 2015 Feb;359(2):679-92. (IF 4.044)

38. Yang X, Ndawula C Jr, Zhou H, Gong X, Jin J.
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Oncol Lett. 2015 Feb;9(2):757-761. (IF 2.311)

39. Luo B, Ju S, Muneri CW, Rui R.
Effects of histone acetylation status on the early development of in vitro porcine transgenic cloned embryos.
Cell Reprogram. 2015 Feb;17(1):41-8. (IF 1.702)

40. Guo Y, Liu Q, Yang Y, Guo X, Lian R, Li S, Wang C, Zhang S, Chen J.
The effects of ROCK inhibitor Y-27632 on injectable spheroids of bovine corneal endothelial cells.
Cell Reprogram. 2015 Feb;17(1):77-87. (IF 1.702)

41. Li C, Zhang Y, Wang L, Feng H, Xia X, Ma J, Yuan H, Gao B, Lan X.
A novel multivalent (99m)Tc-labeled EG2-C4bpα antibody for targeting the epidermal growth factor receptor in tumor xenografts.
Nucl Med Biol. 2015 Jun;42(6):547-54. (IF 2.396)

42. Sun LD, Wang F, Dai F, Wang YH, Lin D, Zhou B.
Development and mechanism investigation of a new piperlongumine derivative as a potent anti-inflammatory agent.
Biochem Pharmacol. 2015 Jun 1;95(3):156-69. (IF 4.96)

43. Chen H, Zhang L, Guo Z, Wang Y, He R, Qin Y, Quan F, Zhang Y.
Improving the development of early bovine somatic-cell nuclear transfer embryos by treating adult donor cells with vitamin C.
Mol Reprod Dev. 2015 Nov;82(11):867-79. (IF 2.823)

44. Dai X, Chen H, Chen Y, Wu J, Wang H, Shi J, Fei X, Wang Z, Wang A, Dong J, Lan Q, Huang Q.
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Oncol Rep. 2015 Dec;34(6):2997-3006. (IF 3.417)

45. Li F, Feng J, Gao D, Wang J, Song C, Wei S, Qiao M.
Shuyu Capsules Relieve Premenstrual Syndrome Depression by Reducing 5-HT3AR and 5-HT3BRExpression in the Rat Brain.
Neural Plast. 2016;2016:7950781. (IF 3.093)

46. Xiang S, Zeng Y, Xiong B, Qin Y, Huang X, Jiang Y, Luo W, Sooranna SR, Pinhu L.
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J INFLAMM-LOND. 2016 Jun 10;13:19. (IF 2.8)

47. Gao J, Meng Q, Zhao Y, Chen X, Cai L.
EHD1 confers resistance to cisplatin in non-small cell lung cancer by regulating intracellular cisplatinconcentrations.
BMC Cancer. 2016 Jul 13;16:470. (IF 3.15)

48. Chen H, Zhang L, Deng T, Zou P, Wang Y, Quan F, Zhang Y.
Effects of oocyte vitrification on epigenetic status in early bovine embryos.
Theriogenology. 2016 Aug;86(3):868-78. (IF 2.094)

49. Zhang L, Xie D, Chen X, Hughes ML, Jiang G, Lu Z, Xia C, Li L, Wang J, Xu W, Sun Y, Li R, Wang R, Qian F, Li J, Li J.
p53 Mediates Colistin-Induced Autophagy and Apoptosis in PC-12 Cells.
ANTIMICROB AGENTS CH. 2016 Aug 22;60(9):5294-301. (IF 4.904)

50. Li H, Chen L, Zhang M, Zhang B.
Foxa1 gene and protein in developing rat eccrine sweat glands.
J Mol Histol. 2016 Oct 27. [Epub ahead of print] (IF 2.531)

51. Huang X, Huan P, Liu B.
A comparative proteomic analysis reveals important proteins for the fertilization and earlyembryonic development of the oyster Crassostrea gigas.
Proteomics. 2017 Jan;17(1-2). (IF 3.254)

52. He J, Li X, Luo H, Li T, Zhao L, Qi Q, Liu Y, Yu Z.
Galectin-3 mediates the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with NADPH oxidase 4.
J Am Soc Hypertens. 2017 May;11(5):275-289.e2. (IF 2.268)

53. Wang ZB, Zhang S, Li Y, Wang RM, Tong LC, Wang Y, Liu WY, Su DF, Tu Y, Zhang LC, Li L.
LY333531, a PKCβ inhibitor, attenuates glomerular endothelial cell apoptosis in the early stage of mouse diabetic nephropathy via down-regulating swiprosin-1.
Acta Pharmacol Sin. 2017 Jul;38(7):1009-1023. (IF 5.064)

54. Zhao X, Wang R, Xiong J, Yan D, Li A, Wang S, Xu J, Zhou J.
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Toxicol Lett. 2017 Aug 5;277:32-40. (IF 3.569)

55. Li W, Mao L, Cao Y, Zhou B, Yang L, Han L, Hao F, Lin T, Zhang W, Jiang J.
Porcine Viperin protein inhibits the replication of classical swine fever virus (CSFV) in vitro.
Virol J. 2017 Oct 23;14(1):202. (IF 2.579)

56. Zhang DD, Gao XM, Zhao YQ, Hou CC, Zhu JQ.
The C-terminal kinesin motor KIFC1 may participate in nuclear reshaping and flagellum formation during spermiogenesis of Larimichthys crocea.
Fish Physiol Biochem. 2017 Oct;43(5):1351-1371. (IF 2.242)

57. Luo T, Guo T, Yang Q, Hao S, Wang J, Cheng Z, Qu Q, He Y, Gong Y, Gao F, Li W, Xia H, Wang B.
In situ hydrogels enhancing postoperative functional recovery by reducing iron overload afterintracerebral haemorrhage.
INT J PHARMACOL. 2017 Dec 20;534(1-2):179-189. (IF 0.692)

58. Zhu Y, Zhou J, Feng Y, Chen L, Zhang L, Yang F, Zha H, Wang X, Han X, Shu C, Wan YY, Li QJ, Guo B, Zhu B
Control of Intestinal Inflammation, Colitis-Associated Tumorigenesis, and Macrophage Polarization by Fibrinogen-Like Protein 2.
Front Immunol. 2018 Jan 30;9:87. (IF 5.085)

59. Huang Y, Jiang T, Chen J, Yin GY, Fan J
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Int J Mol Med. 2018 Feb;41(2):749-756 (IF 3.098)

60. He W, Mosselhy DA, Zheng Y, Feng Q, Li X, Yang X, Yue L, Hannula SP
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INT J NANOMED. 2018 Feb 9;13:877-893. (IF 5.115)

61. Zhan Y, He Z, Liu X, Miao N, Lin F, Xu W, Mu S, Mu H, Yuan M, Cao X, Jin H, Liu Z, Li Y, Zhang B
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INT J BIOCHEM CELL B. 2018 May; (IF 3.673)

62. Zhao YQ, Mu DL, Wang D, Han YL, Hou CC, Zhu JQ
Analysis of the function of KIF3A and KIF3B in the spermatogenesis in Boleophthalmus pectinirostris.
Fish Physiol Biochem. 2018 Jun;44(3):769-788. (IF 2.242)

63. Yang SQ, Chen YD, Li H, Hui X, Gao WY
Geniposide and Gentiopicroside Suppress Hepatic Gluconeogenesis via Regulation of AKT-FOXO1 Pathway.
Arch Med Res. 2018 Jul;49(5):314-322. (IF 2.093)

64. Li H, Chen L, Zhang M, Xie S, Cheng L
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Acta Histochem. 2018 Aug;120(6):520-524. (IF 2.107)

65. Ouyang Z, Li HH, Zhang MJ, Xie ST, Cheng LH
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CHINESE MED J-PEKING. 2018 Aug 20;131(16):1964-1968. (IF 1.585)

66. Liang Y, Chen B, Liu F, Wang J, Yang Y, Zheng Y, Tan S.
Shank-associated RH domain-interacting protein expression is upregulated in entodermal and mesodermal cancer or downregulated in ectodermal malignancy.
Oncol Lett. 2018 Dec;16(6):7180-7188. (IF 2.311)

67. Peng LY, An L, Sun NY, Ma Y, Zhang XW, Liu WH, Liu BL, Li P, Chen J
Salvia miltiorrhiza Restrains Reactive Oxygen Species-Associated Pulmonary Fibrosis via Targeting Nrf2-Nox4 Redox Balance.
AM J CHINESE MED. 2019;47(5):1113-1131. (IF 3.682)

68. Zhong Y, Zhu Y, He T, Li W, Li Q, Miao Y
Brain-derived neurotrophic factor inhibits hyperglycemia-induced apoptosis and downregulation of synaptic plasticity-related proteins in hippocampal neurons via the PI3K/Akt pathway.
Int J Mol Med. 2019 Jan;43(1):294-304. (IF 3.098)

69. Gao XM, Mu DL, Hou CC, Zhu JQ, Jin S, Wang CL
Expression and putative functions of KIFC1 for nuclear reshaping and midpiece formation during spermiogenesis of Phascolosoma esculenta.
Gene. 2019 Jan 30;683:169-183. (IF 2.984)

70. Song S, Liu L, Yu Y, Zhang R, Li Y, Cao W, Xiao Y, Fang G, Li Z, Wang X, Wang Q, Zhao X, Chen L, Wang Y, Wang Q
Inhibition of BRD4 attenuates transverse aortic constriction- and TGF-β-induced endothelial-mesenchymal transition and cardiac fibrosis.
J Mol Cell Cardiol. 2019 Feb;127:83-96. (IF 4.133)

71. Di B, Li HW, Li W, Hua B
Liraglutide inhibited AGEs induced coronary smooth muscle cell phenotypic transition through inhibiting the NF-κB signal pathway.
Peptides. 2019 Feb;112:125-132. (IF 2.843)

72. Liu M, Hu Y, Yuan Y, Tian Z, Zhang C
γδT Cells Suppress Liver Fibrosis via Strong Cytolysis and Enhanced NK Cell-Mediated Cytotoxicity Against Hepatic Stellate Cells.
Front Immunol. 2019 Mar 15;10:477. (IF 5.085)

73. Xu J, Qian J, Zhang W, Chen E, Zhang G, Cao G, Wang F, Shen X, Zhou W, Song Z
LYPD8 regulates the proliferation and migration of colorectal cancer cells through inhibiting the secretion of IL‑6 and TNF‑α.
Oncol Rep. 2019 Apr;41(4):2389-2395. (IF 3.417)

74. Luo G, Jian Z, Zhu Y, Zhu Y, Chen B, Ma R, Tang F, Xiao Y
Sirt1 promotes autophagy and inhibits apoptosis to protect cardiomyocytes from hypoxic stress.
Int J Mol Med. 2019 May;43(5):2033-2043. (IF 3.098)

75. Shen W, Jin Z, Tong X, Wang H, Zhuang L, Lu X, Wu S
TRIM14 promotes cell proliferation and inhibits apoptosis by suppressing PTEN in colorectal cancer.
Cancer Manag Res. 2019 Jun 24;11:5725-5735. (IF 2.886)

76. He Y, Moqbel SAA, Xu L, Ran J, Ma C, Xu K, Bao J, Jiang L, Chen W, Xiong Y, Wu L
Costunolide inhibits matrix metalloproteinases expression and osteoarthritis via the NF‑κB and Wnt/β‑catenin signaling pathways.
Mol Med Rep. 2019 Jul;20(1):312-322. (IF 2.1)

77. Ran X, Yang Y, Meng Y, Li Y, Zhou L, Wang Z, Zhu J
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J Chem Neuroanat. 2019 Jul;98:97-103. (IF 2.353)

78. Shi L, Li B, Zhang B, Zhen C, Zhou J, Tang S
Mouse embryonic palatal mesenchymal cells maintain stemness through the PTEN-Akt-mTOR autophagic pathway.
Stem Cell Res Ther. 2019 Jul 29;10(1):217. (IF 5.116)

79. Xiang XJ, Song L, Deng XJ, Tang Y, Min Z, Luo B, Wen QX, Li KY, Chen J, Ma YL, Zhu BL, Yan Z, Chen GJ
Mitochondrial methionine sulfoxide reductase B2 links oxidative stress to Alzheimer’s disease-like pathology.
Exp Neurol. 2019 Aug;318:145-156. (IF 4.691)

80. Lv B, Min S, Xie F, Yang J, Chen J
Alleviating Sepsis-Induced Neuromuscular Dysfunction Linked With Acetylcholine Receptors by Agrin.
J Surg Res. 2019 Sep;241:308-316. (IF 1.841)

81. Fu L, Chang H, Wang Z, Xie X, Chen H, Lei Z, Zhang Y, Quan F
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Cryobiology. 2019 Oct;90:41-46. (IF 2.283)

82. Xu S, Ni H, Chen H, Dai Q
The interaction between STAT3 and nAChRα1 interferes with nicotine-induced atherosclerosis via Akt/mTOR signaling cascade.
AGING-US. 2019 Oct 14;11(19):8120-8138. (IF 4.831)

83. Shen Y, Xu L, Ning Z, Liu L, Lin J, Chen H, Meng Z
ARHGAP4 regulates the cell migration and invasion of pancreatic cancer by the HDAC2/β-catenin signaling pathway.
Carcinogenesis. 2019 Nov 25;40(11):1405-1414. (IF 4.603)

84. Li Q, Yao Y, Shi S, Zhou M, Zhou Y, Wang M, Chiu JJ, Huang Z, Zhang W, Liu M, Wang Q, Tu X
Inhibition of miR-21 alleviated cardiac perivascular fibrosis via repressing EndMT in T1DM.
J Cell Mol Med. 2020 Jan;24(1):910-920. (IF 4.486)

85. Fusheng Si, Xiaoxia Hu, Chenyang Wang, Bingqing Chen, Ruiyang Wang, Shijuan Dong, Ruisong Yu, Zhen Li
Porcine Epidemic Diarrhea Virus (PEDV) ORF3 Enhances Viral Proliferation by Inhibiting Apoptosis of Infected Cells
VIRUSES-BASEL. 2020 Feb 14;12(2):214.;doi: 10.3390/v12020214. (IF 3.816)

86. Zou H, Wang T, Yuan J, Sun J, Yuan Y, Gu J, Liu X, Bian J, Liu Z
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Toxicol Lett. 2020 Mar 15;321:32-43. (IF 3.569)

87. Hui Zou, Tao Wang, Junzhao Yuan, Jian Sun, Yan Yuan, Jianhong Gu, Xuezhong Liu, Jianchun Bian, Zongping Liu
Cadmium-induced cytotoxicity in mouse liver cells is associated with the disruption of autophagic flux via inhibiting the fusion of autophagosomes and lysosomes
Toxicol Lett. 2020 Mar 15;321:32-43.;doi: 10.1016/j.toxlet.2019.12.019. (IF 3.569)

88. Minxia Ke, Meng Ji, Hao Wang, Yifeng Yao, Yuehong Wu, Nianmin Qi
Inhibition of Rho-associated protein kinase improves the survival of human induced pluripotent stem cell-derived cardiomyocytes after dissociation
Exp Ther Med. 2020 Mar;19(3):1701-1710.;doi: 10.3892/etm.2020.8436. (IF 1.785)

89. Zhigang Yi, Yanchuan Pu, Ruoyan Gou, Yonggang Chen, Xiaojun Ren, Wenzhong Liu, Ping Dong
Silencing of RIPK4 inhibits epithelial‑mesenchymal transition by inactivating the Wnt/β‑catenin signaling pathway in osteosarcoma
Mol Med Rep. 2020 Mar;21(3):1154-1162.;doi: 10.3892/mmr.2020.10939. (IF 2.1)

90. Cui L, Wang H, Lin J, Wang Y, Dong J, Li J, Li J
Progesterone inhibits inflammatory response in E.coli- or LPS-Stimulated bovine endometrial epithelial cells by NF-κB and MAPK pathways.
Dev Comp Immunol. 2020 Apr;105:103568. (IF 3.192)

91. Luying Cui, Heng Wang, Jiaqi Lin, Yali Wang, Junsheng Dong, Jun Li, Jianji Li
Progesterone inhibits inflammatory response in E.coli- or LPS-Stimulated bovine endometrial epithelial cells by NF-κB and MAPK pathways
Dev Comp Immunol. 2020 Apr;105:103568.;doi: 10.1016/j.dci.2019.103568. (IF 3.192)

92. Zhen-Yu She, Yue-Ling Li, Yang Lin, Ming-Hui Lu, Ya-Lan Wei, Kai-Wei Yu, Ning Zhong, Yu Xiao
Kinesin-6 family motor KIF20A regulates central spindle assembly and acrosome biogenesis in mouse spermatogenesis
BBA-MOL CELL RES. 2020 Apr;1867(4):118636.;doi: 10.1016/j.bbamcr.2019.118636. (IF 4.105)

93. Jing Zhang, Yunhai Dai, Chao Wei, Xiaowen Zhao, Qingjun Zhou, Lixin Xie
DNase I improves corneal epithelial and nerve regeneration in diabetic mice
J Cell Mol Med. 2020 Apr;24(8):4547-4556.;doi: 10.1111/jcmm.15112. (IF 4.486)

94. Zhihong Shao, Xiaolong Ma, Yufeng Zhang, Yuanyuan Sun, Wenjuan Lv, Kuigang He, Rui Xia, Peijun Wang, Xiaolong Gao
CPNE1 predicts poor prognosis and promotes tumorigenesis and radioresistance via the AKT singling pathway in triple-negative breast cancer
MOL CARCINOGEN. 2020 May;59(5):533-544.;doi: 10.1002/mc.23177. (IF 3.825)

95. Min Yu, Ni Zheng, Dudu Jiang, Lijing Wang, Qing Zhan, Jiangmin Zhao
Chemokine C-C motif ligand 2 suppressed the growth of human brain astrocytes under Ischemic/hypoxic conditions via regulating ERK1/2 pathway
BRAIN INJURY. 2020 Jul 28;34(9):1277-1282.;doi: 10.1080/02699052.2020.1797167. (IF 1.69)

96. Rezvan Moallemian, Ashfaq Ur Rehman, Na Zhao, Huan Wang, Haifeng Chen, Gang Lin, Xiaojing Ma, Jing Yu
Immunoproteasome inhibitor DPLG3 attenuates experimental colitis by restraining NF-κB activation
Biochem Pharmacol. 2020 Jul;177:113964.;doi: 10.1016/j.bcp.2020.113964. (IF 4.96)

97. Xiao Chen, Xuliang Chen, Xiangxiang Shi, Zhan Gao, Zhigang Guo
Curcumin attenuates endothelial cell fibrosis through inhibiting endothelial-interstitial transformation
CLIN EXP PHARMACOL P . 2020 Jul;47(7):1182-1192.;doi: 10.1111/1440-1681.13271. (IF 2.092)

98. Yan Zhang, Bao-You Fan, Yi-Lin Pang, Wen-Yuan Shen, Xu Wang, Chen-Xi Zhao, Wen-Xiang Li, Chang Liu, Xiao-Hong Kong, Guang-Zhi Ning, Shi-Qing Feng, Xue Yao
Neuroprotective effect of deferoxamine on erastininduced ferroptosis in primary cortical neurons
Neural Regen Res. 2020 Aug;15(8):1539-1545.;doi: 10.4103/1673-5374.274344. (IF 3.171)

99. Yi Du, Yi-Tong Yang, Gang Tang, Jie-Shuang Jia, Nan Zhu, Wei-Jie Yuan
Butyrate alleviates diabetic kidney disease by mediating the miR-7a-5p/P311/TGF-β1 pathway
FASEB J. 2020 Aug;34(8):10462-10475.;doi: 10.1096/fj.202000431R. (IF 4.966)

100. Enhui Fu, Tianyang Liu, Siyang Yu, Xiaohang Chen, Lianhao Song, Huihuang Lou, Fen Ma, Siwei Zhang, Sajjad Hussain, Junnan Guo, Ji Sun, Pingyang Yu, Xionghui Mao, Lanlan Wei
M2 macrophages reduce the radiosensitivity of head and neck cancer by releasing HB‑EGF
Oncol Rep. 2020 Aug;44(2):698-710.;doi: 10.3892/or.2020.7628. (IF 3.417)

101. Xinmin Zhao, Xianghua Wu, Huijie Wang, Hui Yu, Jialei Wang
USP53 promotes apoptosis and inhibits glycolysis in lung adenocarcinoma through FKBP51-AKT1 signaling
MOL CARCINOGEN. 2020 Aug;59(8):1000-1011.;doi: 10.1002/mc.23230. (IF 3.825)

102. Pei Zhang, Yimin Wu, Ruifeng Li, Huicheng Lv, Baolong Yu
Tripartite Motif Containing 52 Positively Regulates NF-κB Signaling by Promoting IκBα Ubiquitination in Lipopolysaccharide-Treated Microglial Cell Activation
MED SCI MONITOR. 2020 Oct 30;26:e925356.;doi: 10.12659/MSM.925356. (IF 1.918)

103. Ensheng Zhang, Jing Wang, Qian Chen, Zhaohao Wang, Dong Li, Ning Jiang, Xiuli Ju
Artesunate ameliorates sepsis-induced acute lung injury by activating the mTOR/AKT/PI3K axis
Gene. 2020 Oct 30;759:144969.;doi: 10.1016/j.gene.2020.144969. (IF 2.984)

104. Qi-Feng Chen, Feng Shi, Tao Huang, Chaoyun Huang, Lujun Shen, Peihong Wu, Wang Li
ASTN1 is associated with immune infiltrates in hepatocellular carcinoma, and inhibits the migratory and invasive capacity of liver cancer via the Wnt/β‑catenin signaling pathway
Oncol Rep. 2020 Oct;44(4):1425-1440.;doi: 10.3892/or.2020.7704. (IF 3.417)

105. Anzana Parvin, Shuang-Li Hao, Fu-Qing Tan, Wan-Xi Yang
Inhibition of kinesin motor protein KIFC1 by AZ82 induces multipolar mitosis and apoptosis in prostate cancer cell
Gene. 2020 Nov 15;760:144989.;doi: 10.1016/j.gene.2020.144989. (IF 2.984)

106. Hui Zou, Junzhao Yuan, Yi Zhang, Tao Wang, Yan Chen, Yan Yuan, Jianchun Bian, Zongping Liu
Gap Junction Intercellular Communication Negatively Regulates Cadmium-Induced Autophagy and Inhibition of Autophagic Flux in Buffalo Rat Liver 3A Cells
Front Pharmacol. 2020 Nov 26;11:596046.;doi: 10.3389/fphar.2020.596046. (IF 4.225)

107. Xue Zhou, Jie Liang, Jiang Wang, Zhaoyang Fei, Guangcheng Qin, Dunke Zhang, Jiying Zhou, Lixue Chen
Up-regulation of astrocyte excitatory amino acid transporter 2 alleviates central sensitization in a rat model of chronic migraine
J Neurochem. 2020 Nov;155(4):370-389.;doi: 10.1111/jnc.14944. (IF 4.066)

108. Xiaobo Wang, Xuxing Ye, Yili Zhang, Feng Ji
Flurbiprofen suppresses the inflammation, proliferation, invasion and migration of colorectal cancer cells via COX2
Oncol Lett. 2020 Nov;20(5):132.;doi: 10.3892/ol.2020.11993. (IF 2.311)

109. Xiaohong Zhao, Changping Gu, Yuelan Wang
PAD4 selective inhibitor TDFA protects lipopolysaccharide-induced acute lung injury by modulating nuclear p65 localization in epithelial cells
Int Immunopharmacol. 2020 Nov;88:106923.;doi: 10.1016/j.intimp.2020.106923. (IF 3.943)

110. Zhaohui Liu, Yanli Meng, Yu Miao, Lili Yu, Qiannan Yu
Propofol reduces renal ischemia/reperfusion-induced acute lung injury by stimulating sirtuin 1 and inhibiting pyroptosis
AGING-US. 2020 Dec 1;13(1):865-876.;doi: 10.18632/aging.202191. (IF 4.831)

111. Kexin Hua, Huimin Gong, Qingrong Xu, Tingting Li, Bin Ma, Yangjie Li, Rongrong He, Dingren Bi, Rui Zhou, Rui Luo, Ling Zhao, Hui Jin
P38 mitogen-activated protein kinase promotes Wnt/β-catenin signaling by impeding Dickkofp-1 expression during Haemophilus parasuis infection
Cytokine. 2020 Dec;136:155287.;doi: 10.1016/j.cyto.2020.155287. (IF 2.952)

112. Xin Xue, Liang Zhang, Xiang Yin, Xing-Xing Chen, Zong-Feng Chen, Chen-Xu Wang, Yu Xiang, Ming-Yong Liu, Jian-Hua Zhao
Transplantation of neural stem cells preconditioned with high‑mobility group box 1 facilitates functional recovery after spinal cord injury in rats
Mol Med Rep. 2020 Dec;22(6):4725-4733.;doi: 10.3892/mmr.2020.11565. (IF 2.1)

113. Xiang-Rong Li, Xiaju Cheng, Jia Sun, Yan S Xu, Nannan Chen, Yimei Hao, Chao Huang, Y Eugene Chin
Acetylation-dependent glutamate receptor GluR signalosome formation for STAT3 activation in both transcriptional and metabolism regulation
Cell Death Discov. 2021 Jan 14;7(1):11.;doi: 10.1038/s41420-020-00389-6.

114. Hongfei Ge, Chao Zhang, Yang Yang, Weixiang Chen, Jun Zhong, Xuanyu Fang, Xuheng Jiang, Liang Tan, Yongjie Zou, Rong Hu, Yujie Chen, Hua Feng
Ambroxol Upregulates Glucocerebrosidase Expression to Promote Neural Stem Cells Differentiation Into Neurons Through Wnt/β-Catenin Pathway After Ischemic Stroke
Front Mol Neurosci. 2021 Jan 20;13:596039.;doi: 10.3389/fnmol.2020.596039. (IF 4.057)

115. Litao Yan, Gejun Liu, Xing Wu
The umbilical cord mesenchymal stem cell-derived exosomal lncRNA H19 improves osteochondral activity through miR-29b-3p/FoxO3 axis
Clin Transl Med. 2021 Jan;11(1):e255.;doi: 10.1002/ctm2.255.

116. Chunguang Li, Maoling Zhu, Ji Zhu, Qijue Lu, Bowen Shi, Bin Sun, Hezhong Chen
SOX12 contributes to the activation of the JAK2/STAT3 pathway and malignant transformation of esophageal squamous cell carcinoma
Oncol Rep. 2021 Jan;45(1):129-138.;doi: 10.3892/or.2020.7863. (IF 3.417)

117. Jing-Wei Xing, Man-Man Chen, Xin-Yun Tian, Dan-Qing Pan, Xiu-Hua Peng, Peng-Fei Gao
919 syrup inhibits ROS-mediated leptin-induced anorexia by activating PPARγ and improves gut flora abnormalities
Biomed Pharmacother. 2021 Jun;138:111455.;doi: 10.1016/j.biopha.2021.111455. (IF 4.545)

118. Jianwen Hao, Qizhen Peng, Keruo Wang, Ge Yu, Yi Pan, Xiaoling Du, Na Hu, Xuening Zhang, Yu Qin, Huikai Li
Antitumor Effect of Lenvatinib Combined with Alisertib in Hepatocellular Carcinoma by Targeting the DNA Damage Pathway
Biomed Res Int. 2021 Jul 22;2021:6613439.;doi: 10.1155/2021/6613439. (IF 2.276)

119. Yujie Zhao, Yuan Chen, Jiayi Wang, Xue Zhu, Ke Wang, Yue Li, Fanfan Zhou
Ginkgolide J protects human synovial cells SW982 via suppression of p38‑dependent production of pro‑inflammatory mediators
Mol Med Rep. 2021 Aug;24(2):555.;doi: 10.3892/mmr.2021.12194. (IF 2.1)

120. Jing Sun, Wugui Chen, Songtao Li, Sizhen Yang, Ying Zhang, Xu Hu, Hao Qiu, Jigong Wu, Shangcheng Xu, Tongwei Chu
Nox4 Promotes RANKL-Induced Autophagy and Osteoclastogenesis via Activating ROS/PERK/eIF-2α/ATF4 Pathway
Front Pharmacol. 2021 Sep 28;12:751845.;doi: 10.3389/fphar.2021.751845. (IF 4.225)

121. Na Zhang, Gang Wang, Gengyun Sun
Actin-binding protein, IQGAP1, regulates LPS-induced RPMVECs hyperpermeability and ICAM-1 upregulation via Rap1/Src signalling pathway
Cell Signal. 2021 Sep;85:110067.;doi: 10.1016/j.cellsig.2021.110067. (IF 3.968)

122. Rui Wang, Jian Liu, Kai Li, Ganghua Yang, Sisi Chen, Jie Wu, Xinming Xie, Hong Ren, Yamei Pang
An SETD1A/Wnt/β-catenin feedback loop promotes NSCLC development
J Exp Clin Cancer Res. 2021 Oct 13;40(1):318.;doi: 10.1186/s13046-021-02119-x.

123. Xiaolei Liu, Shaoping Lin, Yiyue Zhong, Jiaojiao Shen, Xuedi Zhang, Shuhua Luo, Li Huang, Liangqing Zhang, Shuangnan Zhou, Jing Tang
Remimazolam Protects Against LPS-Induced Endotoxicity Improving Survival of Endotoxemia Mice
Front Pharmacol. 2021 Nov 19;12:739603.;doi: 10.3389/fphar.2021.739603. (IF 4.225)

124. Bing-Xin Chu, Ya-Nan Li, Ning- Liu, Lan-Xin Yuan, Shi-Yan Chen, Yao-Hong Zhu, Jiu-Feng Wang
Salmonella Infantis Delays the Death of Infected Epithelial Cells to Aggravate Bacterial Load by Intermittent Phosphorylation of Akt With SopB
Front Immunol. 2021 Nov 5;12:757909.;doi: 10.3389/fimmu.2021.757909. (IF 5.085)

125. Yu Zhou, Wei-Jiang Zhao, Wei Quan, Chen-Meng Qiao, Chun Cui, Hui Hong, Yun Shi, Gu-Yu Niu, Li-Ping Zhao, Yan-Qin Shen
Dynamic changes of activated AHR in microglia and astrocytes in the substantia nigra-striatum system in an MPTP-induced Parkinson’s disease mouse model
Brain Res Bull. 2021 Nov;176:174-183.;doi: 10.1016/j.brainresbull.2021.08.013. (IF 3.37)

126. Jinshan Wang, Shizhen Jin, Wenshuang Fu, Yufeng Liang, Yani Yang, Xiaohong Xu
Pubertal exposure to bisphenol-A affects social recognition and arginine vasopressin in the brain of male mice
Ecotoxicol Environ Saf. 2021 Dec 15;226:112843.;doi: 10.1016/j.ecoenv.2021.112843.

127. Lin Lei, Wenwen Gao, Juan J Loor, Ahmad Aboragah, Zhiyuan Fang, Xiliang Du, Min Zhang, Yuxiang Song, Guowen Liu, Xinwei Li
Reducing hepatic endoplasmic reticulum stress ameliorates the impairment in insulin signaling induced by high levels of β-hydroxybutyrate in bovine hepatocytes
J Dairy Sci. 2021 Dec;104(12):12845-12858.;doi: 10.3168/jds.2021-20611. (IF 3.333)

128. Siwen Zhang, Junfu Wang, Ting Chen, Jiancheng Wang, Ye Wang, Zhu Yu, Kun Zhao, Kaitian Zheng, Yeyang Chen, Zhen Wang, Bopei Li, Congjun Wang, Weijia Huang, Zhao Fu, Junqiang Chen
α-Actinin1 promotes tumorigenesis and epithelial-mesenchymal transition of gastric cancer via the AKT/GSK3β/β-Catenin pathway
Bioengineered. 2021 Dec;12(1):5688-5704.;doi: 10.1080/21655979.2021.1967713. (IF 2.205)

129. Qianqian Li, Ziguan Zhang, Shanshan Chen, Zhengrong Huang, Mengru Wang, Mengchen Zhou, Chenguang Yu, Xiangyi Wang, Yilin Chen, Dan Jiang, Dunfeng Du, Yufeng Huang, Xin Tu, Zhishui Chen, Yuanyuan Zhao
miR-190a-5p Partially Represses the Abnormal Electrical Activity of SCN3B in Cardiac Arrhythmias by Downregulation of IL-2
Front Cardiovasc Med. 2022 Jan 10;8:795675.;doi: 10.3389/fcvm.2021.795675.

130. Shi-Yong Zhu, Jian-Ying Guo, Jin-Yang Li, Xue-Yan Dai, Xue-Nan Li, Jin-Long Li
Lycopene ameliorates atrazine-induced pyroptosis in spleen by suppressing the Ox-mtDNA/Nlrp3 inflammasome pathway
Food Funct. 2022 Mar 21;13(6):3551-3560.;doi: 10.1039/d1fo02857j. (IF 4.171)

131. Chan Zhu, Meiyuan Wang, Jun Guo, Shu Lan Su, Guang Yu, Yan Yang, Yuan Zhou, Zongxiang Tang
Angelica dahurica Extracts Attenuate CFA-Induced Inflammatory Pain via TRPV1 in Mice
Evid Based Complement Alternat Med. 2022 May 23;2022:4684830.;doi: 10.1155/2022/4684830.

132. Chenxi Zhao, Tiangang Zhou, Xiaoqing Zhao, Yilin Pang, Wenxiang Li, Baoyou Fan, Ming Li, Xinjie Liu, Lei Ma, Jiawei Zhang, Chao Sun, Wenyuan Shen, Xiaohong Kong, Xue Yao, Shiqing Feng
Delayed administration of nafamostat mesylate inhibits thrombin-mediated blood-spinal cord barrier breakdown during acute spinal cord injury in rats
J Neuroinflammation. 2022 Jul 16;19(1):189.;doi: 10.1186/s12974-022-02531-w.

Alexa Fluor 555标记驴抗小鼠IgG(H+L)(A0460)

Alexa Fluor 555标记驴抗小鼠IgG(H+L)
Alexa Fluor 555标记驴抗小鼠IgG(H+L)(A0460) 一键复制产品信息

产品编号: A0460

产品包装:100μl
选择包装

100μl

说明书下载

483.00 466.00 10

价格: ¥ 483.00

促销价: ¥ 483.00

促销价: ¥ 483.00 466.00

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产品文件
相关产品
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产品编号 产品名称 产品包装 产品价格
A0460 Alexa Fluor 555标记驴抗小鼠IgG(H+L) 100μl 483.00元

本Alexa Fluor 555标记驴抗小鼠IgG(H+L)(Alexa Fluor 555-labeled Donkey Anti-Mouse IgG(H+L))为进口分装,用于免疫荧光染色。
Alexa Fluor 555是一种常用的非常明亮的红色荧光探针。它比绝大部分常用的红色荧光探针更加明亮,更加不容易淬灭,而且背景更低。Alexa Fluor 555的荧光光谱和Cy3非常接近。Alexa Fluor 555的吸收(激发)和发射峰参见下表。

Fluorophore Absorption Peak (nm) Emission Peak (nm)
Alexa Fluor 555 555 565

本抗体为用纯化的小鼠IgG免疫驴,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人IgG、兔IgG和大鼠IgG吸附纯化的优质二抗。
本Alexa Fluor 555标记驴抗小鼠IgG(H+L)用于免疫荧光染色时的推荐稀释比例为1:500。实际实验操作过程中需根据抗原和抗体的具体情况适当调节荧光标记二抗的稀释比例,推荐的调节范围为1:200-1000。
本抗体如果用于常规的免疫染色,以每次检测需1毫升1:500稀释的荧光标记二抗计,至少可以检测50次。如果适当重复使用已经使用过的荧光标记二抗,至少可以多检测150-250次。
包装清单:

产品编号 产品名称 包装
A0460 Alexa Fluor 555标记驴抗小鼠IgG (H+L) 100μl
说明书 1份

保存条件:
-20℃避光保存,一年有效。
注意事项:
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 免疫荧光染色请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
2. 如果希望重复使用稀释的荧光标记二抗,稀释的荧光标记二抗4℃保存。

Alexa Fluor 555标记驴抗小鼠IgG(H+L)(A0460)
Alexa Fluor 555标记驴抗小鼠IgG(H+L)(A0460)

物质安全数据单
输入右侧验证码点击下载:

Alexa Fluor 555标记驴抗小鼠IgG(H+L)(A0460)

Alexa Fluor 555标记驴抗小鼠IgG(H+L)(A0460)
质检证书
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相关产品请点击如下按钮:
荧光标记二抗

使用本产品的文献:

1. Zhao H, Chen Y, Shao L, Xie M, Nie J, Qiu J, Zhao P, Ramezani H, Fu J, Ouyang H, He Y
Airflow-Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle.
Small. 2018 Sep;14(39):e1802630. (IF 11.459)

2. Shuang Qiao, Wenhua Lu, Christophe Glorieux, Jiangjiang Li, Peiting Zeng, Ning Meng, Huiqin Zhang, Shijun Wen, Peng Huang
Wild-type IDH2 protects nuclear DNA from oxidative damage and is a potential therapeutic target in colorectal cancer
Oncogene. 2021 Sep;40(39):5880-5892.;doi: 10.1038/s41388-021-01968-2. (IF 7.971)

3. Li F, Yang X, Liu J, Shu K, Shen C, Chen T, Yang W, Li S, Wang X, Jiang M
Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus.
MOL THER-NUCL ACIDS. 2019 Jun 7;16:295-301. (IF 7.032)

4. Xiao T, Zhong W, Zhao J, Qian B, Liu H, Chen S, Qiao K, Lei Y, Zong S, Wang H, Liang Y, Zhang H, Meng J, Zhou H, Sun T, Liu Y, Yang C
Polyphyllin I suppresses the formation of vasculogenic mimicry via Twist1/VE-cadherin pathway.
Cell Death Dis. 2018 Sep 5;9(9):906. (IF 6.304)

5. Mengyun Wang, Yue Du, Song Gao, Zheng Wang, Pengxiang Qu, Yang Gao, Jingyi Wang, Zhengqi Liu, Jingcheng Zhang, Yong Zhang, Suzhu Qing, Yongsheng Wang
Sperm-borne miR-202 targets SEPT7 and regulates first cleavage of bovine embryos via cytoskeletal remodeling
Development. 2021 Mar 5;148(5):dev189670.;doi: 10.1242/dev.189670. (IF 5.611)

6. Guan Y, Guo L, Yang E, Liao Y, Liu L, Che Y, Zhang Y, Wang L, Wang J, Li Q.
HSV-1 nucleocapsid egress mediated by UL31 in association with UL34 is impeded by cellular transmembrane protein 140.
Virology. 2014 Jul 15;464-465C:1-10. (IF 2.819)

7. Dong WL, Hou CC, Yang WX.
Mitochondrial prohibitin and its ubiquitination during crayfish Procambarus clarkii spermiogenesis.
Cell Tissue Res. 2015 Feb;359(2):679-92. (IF 4.044)

8. Hu L, Su C, Song X, Shi Q, Fu J, Xia X, Xu D, Song E, Song Y.
Tetrachlorobenzoquinone triggers the cleavage of Bid and promotes the cross-talk of extrinsic and intrinsic apoptotic signalings in pheochromocytoma (PC) 12 cells.
Neurotoxicology. 2015 Jul;49:149-57. (IF 3.105)

9. Wang Y, Tu L, Li Y, Chen D, Wang S .
Notoginsenoside R1 Protects against Neonatal Cerebral Hypoxic-Ischemic Injury through EstrogenReceptor-Dependent Activation of Endoplasmic Reticulum Stress Pathways.
J Pharmacol Exp Ther. 2016 Jun;357(3):591-605. (IF 3.561)

10. Lin Z, Zhu X, Zhang T, You F, Wu Z, Cao Y .
Effects of hydrostatic pressure on microtubule organization and nucleus changes in gynogeneticallyactivated eggs of olive flounder (Paralichthys olivaceus).
Theriogenology. 2016 Jun;85(9):1610-24. (IF 2.094)

11. Shen HQ, Xiao YX, She ZY, Tan FQ, Yang WX.
A novel role of KIF3b in the seminoma cell cycle.
Exp Cell Res. 2017 Mar 1;352(1):95-103. (IF 3.383)

12. Qu P, Qing S, Liu R, Qin H, Wang W, Qiao F, Ge H, Liu J, Zhang Y, Cui W, Wang Y.
Effects of embryo-derived exosomes on the development of bovine cloned embryos.
PLoS One. 2017 Mar 28;12(3). (IF 2.74)

13. Luo C, Chen S, Xu N, Sai WB, Zhao W, Li YC, Hu XJ, Tian H, Gao XD, Yao WB.
Establishment of a fluorescence-based method to evaluate endocytosis of desialylatedglycoproteins in vitro.
Biomed Pharmacother. 2017 Apr;88:87-94. (IF 4.545)

14. Guo J, Cai H, Zheng J, Liu X, Liu Y, Ma J, Que Z, Gong W, Gao Y, Tao W, Xue Y.
Long non-coding RNA NEAT1 regulates permeability of the blood-tumor barrier via miR-181d-5p-mediated expression changes in ZO-1, occludin, and claudin-5.
BBA-BIOMEMBRANES. 2017 Sep;1863(9):2240-2254. (IF 3.411)

15. Xu Y, Li R, Zhang K, Wu W, Wang S, Zhang P, Xu H
The multifunctional RNA-binding protein hnRNPK is critical for the proliferation and differentiation of myoblasts.
BMB Rep. 2018 Jul;51(7):350-355. (IF 3.167)

16. Wu K, Zhang H, Fu Y, Zhu Y, Kong L, Chen L, Zhao F, Yu L, Chen X
TLR4/MyD88 signaling determines the metastatic potential of breast cancer cells.
Mol Med Rep. 2018 Sep;18(3):3411-3420. (IF 2.1)

17. Hua K, Li Y, Zhou H, Hu X, Chen Y, He R, Luo R, Zhou R, Bi D, Jin H
Haemophilus parasuis Infection Disrupts Adherens Junctions and Initializes EMT Dependent on Canonical Wnt/β-Catenin Signaling Pathway.
FRONT CELL INFECT MI. 2018 Sep 12;8:324. (IF 4.123)

18. Li, Wang H, Li Z, Wang C, Xiao F, Gao Y, Zhang X, Wang P, Peng J, Cai G, Zuo B, Shen Y, Qi J, Qian N, Deng L, Song W, Zhang X, Shen L, Chen X
The inhibition of RANKL expression in fibroblasts attenuate CoCr particles induced aseptic prosthesis loosening via the MyD88-independent TLR signaling pathway.
BIOCHEM BIOPH RES CO. 2018 Sep 5;503(2):1115-1122. (IF 2.985)

19. Shen W, Jin Z, Tong X, Wang H, Zhuang L, Lu X, Wu S
TRIM14 promotes cell proliferation and inhibits apoptosis by suppressing PTEN in colorectal cancer.
Cancer Manag Res. 2019 Jun 24;11:5725-5735. (IF 2.886)

20. Mei Li, Di Zhang, Chuntian Li, Zifeng Zheng, Ming Fu, Fengfeng Ni, Yalan Liu, Tao Du, Hanzhong Wang, George E Griffin, Mudan Zhang, Qinxue Hu
Characterization of Zika Virus Endocytic Pathways in Human Glioblastoma Cells
Front Microbiol. 2020 Mar 6;11:242.;doi: 10.3389/fmicb.2020.00242. (IF 4.235)

21. Minxia Ke, Meng Ji, Hao Wang, Yifeng Yao, Yuehong Wu, Nianmin Qi
Inhibition of Rho-associated protein kinase improves the survival of human induced pluripotent stem cell-derived cardiomyocytes after dissociation
Exp Ther Med. 2020 Mar;19(3):1701-1710.;doi: 10.3892/etm.2020.8436. (IF 1.785)

22. Zhen-Yu She, Yue-Ling Li, Yang Lin, Ming-Hui Lu, Ya-Lan Wei, Kai-Wei Yu, Ning Zhong, Yu Xiao
Kinesin-6 family motor KIF20A regulates central spindle assembly and acrosome biogenesis in mouse spermatogenesis
BBA-MOL CELL RES. 2020 Apr;1867(4):118636.;doi: 10.1016/j.bbamcr.2019.118636. (IF 4.105)

23. Anzana Parvin, Shuang-Li Hao, Fu-Qing Tan, Wan-Xi Yang
Inhibition of kinesin motor protein KIFC1 by AZ82 induces multipolar mitosis and apoptosis in prostate cancer cell
Gene. 2020 Nov 15;760:144989.;doi: 10.1016/j.gene.2020.144989. (IF 2.984)

24. Hao Li, Pengfei Cui, Xue Fu, Lan Zhang, Wenjun Yan, Yaru Zhai, Changwei Lei, Hongning Wang, Xin Yang
Identification and analysis of long non-coding RNAs and mRNAs in chicken macrophages infected with avian infectious bronchitis coronavirus
BMC Genomics. 2021 Jan 20;22(1):67.;doi: 10.1186/s12864-020-07359-3. (IF 3.594)

25. Xuechao Jiang, Tingting Li, Bojian Li, Wei Wei, Fen Li, Sun Chen, Rang Xu, Kun Sun
SOX7 suppresses endothelial-to-mesenchymal transitions by enhancing VE-cadherin expression during outflow tract development
Clin Sci (Lond). 2021 Mar 26;135(6):829-846.;doi: 10.1042/CS20201496.

26. Zhen-Yu She, Kai-Wei Yu, Ya-Lan Wei, Ning Zhong, Yang Lin
Kinesin-7 CENP-E regulates the formation and structural maintenance of the acrosome
Cell Tissue Res. 2021 Mar;383(3):1167-1182.;doi: 10.1007/s00441-020-03341-3. (IF 4.044)

27. Xinming Gao, Chen Du, Xuebin Zheng, Junquan Zhu, Shan Jin
Process of cytoplasm elimination during spermiogenesis in Octopus tankahkeei: Polarized development of the spermatid and discarding of the residual body
J Morphol. 2021 Apr;282(4):500-510.;doi: 10.1002/jmor.21323. (IF 1.563)

28. Jing-Wei Xing, Man-Man Chen, Xin-Yun Tian, Dan-Qing Pan, Xiu-Hua Peng, Peng-Fei Gao
919 syrup inhibits ROS-mediated leptin-induced anorexia by activating PPARγ and improves gut flora abnormalities
Biomed Pharmacother. 2021 Jun;138:111455.;doi: 10.1016/j.biopha.2021.111455. (IF 4.545)

29. Wenqi Shan, Weiyue Hu, Ya Wen, Xingwang Ding, Xuan Ma, Wu Yan, Yankai Xia
Evaluation of atrazine neurodevelopment toxicity in vitro-application of hESC-based neural differentiation model
Reprod Toxicol. 2021 Aug;103:149-158.;doi: 10.1016/j.reprotox.2021.06.009. (IF 3.121)

Alexa Fluor 488标记山羊抗小鼠IgG(H+L)(A0428)

Alexa Fluor 488标记山羊抗小鼠IgG(H+L)
Alexa Fluor 488标记山羊抗小鼠IgG(H+L)(A0428) 一键复制产品信息

产品编号: A0428

产品包装:100μl
选择包装

100μl

说明书下载

502.00 485.00 10

价格: ¥ 502.00

促销价: ¥ 502.00

促销价: ¥ 502.00 485.00

产品简介
使用说明
产品文件
相关产品
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产品编号 产品名称 产品包装 产品价格
A0428 Alexa Fluor 488标记山羊抗小鼠IgG(H+L) 100μl 502.00元

本Alexa Fluor 488标记山羊抗小鼠IgG(H+L)(Alexa Fluor 488-labeled Goat Anti-Mouse IgG(H+L))为进口分装,用于免疫荧光染色。
Alexa Fluor 488是一种常用的非常明亮的绿色荧光探针。它比绝大部分常用的绿色荧光探针更加明亮,更加不容易淬灭,而且背景更低。Alexa Fluor 488的荧光光谱与FITC和Cy2比较接近。Alexa Fluor 488的吸收(激发)和发射峰参见下表。

Fluorophore Absorption Peak (nm) Emission Peak (nm)
Alexa Fluor 488 495 519

本抗体为用纯化的小鼠IgG免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人IgG、马IgG、 牛IgG(bovine IgG)、兔IgG和猪IgG吸附纯化的优质二抗。对人IgG、马IgG、牛IgG(bovine IgG)、兔IgG和猪IgG几乎没有结合能力。特别适合于对于二抗种属特异性要求比较高的荧光染色实验。
本Alexa Fluor 488标记山羊抗小鼠IgG(H+L)用于免疫荧光染色时的推荐稀释比例为1:500。实际实验操作过程中需根据抗原和抗体的具体情况适当调节荧光标记二抗的稀释比例,推荐的调节范围为1:200-1000。
本抗体如果用于常规的免疫染色,以每次检测需1毫升1:500稀释的荧光标记二抗计,至少可以检测50次。如果适当重复使用已经使用过的荧光标记二抗,至少可以多检测150-250次。
包装清单:

产品编号 产品名称 包装
A0428 Alexa Fluor 488标记山羊抗小鼠IgG (H+L) 100μl
说明书 1份

保存条件:
-20℃避光保存,一年有效。
注意事项:
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1.免疫荧光染色请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
2.如果希望重复使用稀释的荧光标记二抗,稀释的荧光标记二抗4℃保存。

Alexa Fluor 488标记山羊抗小鼠IgG(H+L)(A0428)
Alexa Fluor 488标记山羊抗小鼠IgG(H+L)(A0428)

物质安全数据单
输入右侧验证码点击下载:

Alexa Fluor 488标记山羊抗小鼠IgG(H+L)(A0428)

Alexa Fluor 488标记山羊抗小鼠IgG(H+L)(A0428)
质检证书
输入批号搜寻COA

搜索

相关产品请点击如下按钮:
荧光标记二抗

使用本产品的文献:

1. Weiliang Mo, Junchuan Zhang, Li Zhang, Zhenming Yang, Liang Yang, Nan Yao, Yong Xiao, Tianhong Li, Yaxing Li, Guangmei Zhang, Mingdi Bian, Xinglin Du, Zecheng Zuo
Arabidopsis cryptochrome 2 forms photobodies with TCP22 under blue light and regulates the circadian clock
Nat Commun. 2022 May 12;13(1):2631.;doi: 10.1038/s41467-022-30231-9. (IF 12.121)

2. Feng Zhu, Hong Zheng, Suilin Chen, Kun Zhang, Xin Qin, Jingru Zhang, Taiping Liu, Yongling Fan, Liting Wang, Xiaoxu Li, Jian Zhang, Wenyue Xu
Malaria oocysts require circumsporozoite protein to evade mosquito immunity
Nat Commun. 2022 Jun 9;13(1):3208.;doi: 10.1038/s41467-022-30988-z. (IF 12.121)

3. Cao L, Chen J, Wang Y, Yang Y, Qing J, Rao Z, Chen X, Lou Z
Identification of serotonin 2A receptor as a novel HCV entry factor by a chemical biology strategy.
Protein Cell. 2019 Mar;10(3):178-195. (IF 10.164)

4. Qidong Li, Hanhui Yao, Yichun Wang, Yang Wu, Rick F Thorne, Youming Zhu, Mian Wu, Lianxin Liu
circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells
Cell Rep. 2022 Nov 22;41(8):111707.;doi: 10.1016/j.celrep.2022.111707. (IF 8.109)

5. Gong Z, Pan J, Shen Q, Li M, Peng Y
Mitochondrial dysfunction induces NLRP3 inflammasome activation during cerebral ischemia/reperfusion injury.
J NEUROINFLAMM. 2018 Aug 28;15(1):242. (IF 5.793)

6. Lin Che, Chuan-Li Yang, Yu Chen, Zi-Li Wu, Ze-Bang Du, Jia-Shen Wu, Cong-Ling Gan, Si-Ping Yan, Jing Huang, Ni-Jun Guo, Yu-Chun Lin, Zhong-Ning Lin
Mitochondrial redox-driven mitofusin 2 S-glutathionylation promotes neuronal necroptosis via disrupting ER-mitochondria crosstalk in cadmium-induced neurotoxicity
Chemosphere. 2021 Jan;262:127878.;doi: 10.1016/j.chemosphere.2020.127878. (IF 5.778)

7. Tong X, Zhang C, Wang D, Song R, Ma Y, Cao Y, Zhao H, Bian J, Gu J, Liu Z
Suppression of AMP-activated protein kinase reverses osteoprotegerin-induced inhibition of osteoclast differentiation by reducing autophagy.
CELL PROLIFERAT. 2020 Jan;53(1):e12714. (IF 5.753)

8. Xishuai Tong, Chuang Zhang, Dong Wang, Ruilong Song, Yonggang Ma, Ying Cao, Hongyan Zhao, Jianchun Bian, Jianhong Gu, Zongping Liu
Suppression of AMP-activated protein kinase reverses osteoprotegerin-induced inhibition of osteoclast differentiation by reducing autophagy
CELL PROLIFERAT. 2020 Jan;53(1):e12714.;doi: 10.1111/cpr.12714. (IF 5.753)

9. Chenliang Zhou, Zhichang Zhang, Xiaoshi Zhu, Guowei Qian, Yan Zhou, Yong Sun, Wenxi Yu, Jiahui Wang, Haiyang Lu, Feng Lin, Zan Shen, Shuier Zheng
N6-Methyladenosine modification of the TRIM7 positively regulates tumorigenesis and chemoresistance in osteosarcoma through ubiquitination of BRMS1
EBioMedicine. 2020 Sep;59:102955.;doi: 10.1016/j.ebiom.2020.102955. (IF 5.736)

10. Wang HY, Li C, Liu WH, Deng FM, Ma Y, Guo LN, Kong H, Hu KA, Liu Q, Wu J, Sun J, Liu YL
Autophagy inhibition via Becn1 downregulation improves the mesenchymal stem cells antifibrotic potential in experimental liver fibrosis.
J Cell Physiol. 2020 Mar;235(3):2722-2737. (IF 5.546)

11. Lu M, Zhuang X, Tang K, Wu P, Guo X, Yin L, Cao H, Zou D
Intrinsic Surface Effects of Tantalum and Titanium on Integrin α5β1/ ERK1/2 Pathway-Mediated Osteogenic Differentiation in Rat Bone Mesenchymal Stromal Cells.
CELL PHYSIOL BIOCHEM. 2018;51(2):589-609. (IF 5.5)

12. Tu C, Li N, Zhu L, Zhou L, Su Y, Li P, Zhu X.
Cationic long-chain hyperbranched poly(ethylene glycol)s with low charge density for gene delivery.
POLYM CHEM-UK. 2013;4:393-401. (IF 5.342)

13. Feng Wei, Sang Ba, Mei Jin, Ren Ci, Xuelian Wang, Fusheng E, Ziwen Long
RNF180 Inhibits Proliferation and Promotes Apoptosis of Colorectal Cancer Through Ubiquitination of WISP1
Front Cell Dev Biol. 2021 Jan 22;8:623455.;doi: 10.3389/fcell.2020.623455. (IF 5.201)

14. Chen C, Wang X, Huang X, Yong H, Shen J, Tang Q, Zhu J, Ni J, Feng Z .
Nicotinamide N-methyltransferase: a potential biomarker for worse prognosis in gastric carcinoma.
Am J Cancer Res. 2016 Feb 15;6(3):649-63. (IF 5.177)

15. Yao B, Song W, Li Z, Hu T, Wang R, Wang Y, Huang S, Fu X
Irf6 directs glandular lineage differentiation of epidermal progenitors and promotes limited sweat gland regeneration in a mouse burn model.
Stem Cell Res Ther. 2018 Jul 4;9(1):179. (IF 5.116)

16. Shi L, Li B, Zhang B, Zhen C, Zhou J, Tang S
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17. Guan X, Zhang M, Fu M, Luo S, Hu Q
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18. Ye Yu, Dong-Ming Wu, Jing Li, Shi-Hua Deng, Teng Liu, Ting Zhang, Miao He, Yang-Yang Zhao, Ying Xu
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41. Li H, Zhang M, Chen L, Zhang B, Zhang C.
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52. Zhang M, Li H, Xie S, Chen L
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53. Yang YL, Ran XR, Li Y, Zhou L, Zheng LF, Han Y, Cai QQ, Wang ZY, Zhu JX
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54. Siyuan Chen, Li Yang, Hui Dong, Hong Guo
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55. Xie T, Xu Q, Wan H, Xing S, Shang C, Gao Y, He Z
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56. Liu H, Wu Y, He F, Cheng Z, Zhao Z, Xiang C, Feng X, Bai X, Takeda S, Wu X, Qing Y
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57. Chen S, Cai F, Wang J, Yang Z, Gu C, Wang G, Mao G, Yan J
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58. Cao L, Chen L, Li H, Wei Z, Xie S, Zhang M, Lin Y, Huang H
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59. Liu B, Liu ZZ, Zhou ML, Lin JW, Chen XM, Li Z, Gao WB, Yu ZD, Liu T
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60. Fu L, Chang H, Wang Z, Xie X, Chen H, Lei Z, Zhang Y, Quan F
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61. Xu S, Ni H, Chen H, Dai Q
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62. Zhu E, Chen W, Qin Y, Ma S, Fan S, Wu K, Li W, Fan J, Yi L, Ding H, Chen J, Zhao M
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63. Xinxin Yang, Jichun Tan, Xiaoyan Xu, Haibo Yang, Fengdi Wu, Bin Xu, Wei Liu, Pengcheng Shi, Zhaofa Xu, Yu Deng
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64. Shuangqi Fan, Keke Wu, Chaowei Luo, Xin Li, Mengpo Zhao, Dan Song, Shengming Ma, Erpeng Zhu, Yuming Chen, Hongxing Ding, Lin Yi, Jun Li, Mingqiu Zhao, Jinding Chen
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65. Liu Y, Wu C, Hou Z, Fu X, Yuan L, Sun S, Zhang H, Yang D, Yao X, Yang J
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66. Miao X, Xiang Y, Mao W, Chen Y, Li Q, Fan B
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67. Fusheng Si, Xiaoxia Hu, Chenyang Wang, Bingqing Chen, Ruiyang Wang, Shijuan Dong, Ruisong Yu, Zhen Li
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68. Jinpeng Lv, Ying Fu, Yan Cao, Songzhou Jiang, Ying Yang, Guoqiang Song, Changjun Yun, Rongyin Gao
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69. Zhen-Yu She, Yue-Ling Li, Yang Lin, Ming-Hui Lu, Ya-Lan Wei, Kai-Wei Yu, Ning Zhong, Yu Xiao
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70. Jialing Peng, Hongxuan Wang, Zhe Gong, Xiangpen Li, Lei He, Qingyu Shen, Jingrui Pan, Ying Peng
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71. Cheng Gao, Haochen Wang, Tao Wang, Chengliang Luo, Zufeng Wang, Mingyang Zhang, Xiping Chen, Luyang Tao
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72. Xiao Chen, Xuliang Chen, Xiangxiang Shi, Zhan Gao, Zhigang Guo
Curcumin attenuates endothelial cell fibrosis through inhibiting endothelial-interstitial transformation
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73. Denglu Zhang, Xiaohang Du, Xufeng Zhang, Kailin Li, Feng Kong, Guanghui Cheng, Shengtian Zhao
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74. Yi Du, Yi-Tong Yang, Gang Tang, Jie-Shuang Jia, Nan Zhu, Wei-Jie Yuan
Butyrate alleviates diabetic kidney disease by mediating the miR-7a-5p/P311/TGF-β1 pathway
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75. Jinpeng Lv, Xiaohong An, Songzhou Jiang, Ying Yang, Guoqiang Song, Rongyin Gao
Protoporphyrin IX Stimulates Melanogenesis, Melanocyte Dendricity, and Melanosome Transport Through the cGMP/PKG Pathway
Front Pharmacol. 2020 Sep 11;11:569368.;doi: 10.3389/fphar.2020.569368. (IF 4.225)

76. Chao Huang, Ke Ming Xiang, Bing Jun Liang, Wei Xuan Huang, Fan Jun Zhang, Yu Wan Shao, Xiu Lian Wang, Hao Sheng Liu, Wei Zeng Shen
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77. Wenhui Li, Zilin Zhang, Liangliang Zhang, Hong Li, Shuangqi Fan, Erpeng Zhu, Jindai Fan, Zhaoyao Li, Wenxian Chen, Lin Yi, Hongxing Ding, Jinding Chen, Mingqiu Zhao
Antiviral Role of Serine Incorporator 5 (SERINC5) Proteins in Classical Swine Fever Virus Infection
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78. Kun-Lin Chen, Yue Wang, Zhi-Ping Lin, Hui-Xia Li
The protective effect of rosmarinic acid on myotube formation during myoblast differentiation under heat stress
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79. Pei Zhang, Yimin Wu, Ruifeng Li, Huicheng Lv, Baolong Yu
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80. Xue Zhou, Jie Liang, Jiang Wang, Zhaoyang Fei, Guangcheng Qin, Dunke Zhang, Jiying Zhou, Lixue Chen
Up-regulation of astrocyte excitatory amino acid transporter 2 alleviates central sensitization in a rat model of chronic migraine
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81. Li Deng, Taiyuan Li, Yuanyuan Liao, Shuang Liu, Zhen Xie, Zhixiang Huang, Hua Dai, Jianfeng Li, Xiong Lei
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82. Zi-Xing Xu, Li-Qun Zhang, Yi-Nan Zhou, Xue-Min Chen, Wei-Hong Xu
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83. Yi R Hu, San L Xing, Chuan Chen, Ding Z Shen, Jiu L Chen
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84. Hóngyi Zhào, Ru Wang, Yan Zhang, Yu Liu, Yonghua Huang
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85. Hongqing Chen, Fei Fei, Xinda Li, Zhenguo Nie, Dezhi Zhou, Libiao Liu, Jing Zhang, Haitao Zhang, Zhou Fei, Tao Xu
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86. Jing-Wei Xing, Man-Man Chen, Xin-Yun Tian, Dan-Qing Pan, Xiu-Hua Peng, Peng-Fei Gao
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87. Jing Qian, Jianyong Zhong, Shaojun Liu, Minhua Yan, Ping Cheng, Chuanming Hao, Yong Gu, Lingyun Lai
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88. He-Qin Zhan, Rong Qin, Yan-Li Li, Meng-Meng Liu, Lin Gan
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89. Yunhui Zhang, Yinru Tang, Yuping Luo, Li Luo, Feihai Shen, Zhiying Huang
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90. Y Lan, W Zeng, X Dong, H Lu
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91. Yu Zhou, Wei-Jiang Zhao, Wei Quan, Chen-Meng Qiao, Chun Cui, Hui Hong, Yun Shi, Gu-Yu Niu, Li-Ping Zhao, Yan-Qin Shen
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92. Dabao Yao, Shuo Zhang, Zhengwei Hu, Haiyang Luo, Chengyuan Mao, Yu Fan, Mibo Tang, Fen Liu, Si Shen, Liyuan Fan, Mengjie Li, Jingjing Shi, Jiadi Li, Dongrui Ma, Yuming Xu, Changhe Shi
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93. Yonglan Ruan, Jinying Ling, Fan Ye, Nuo Cheng, Fei Wu, Zongxiang Tang, Xiaolan Cheng, Hongquan Liu
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94. Manxiu Cao, Junhong Zhao, Lijie Du, Zixiu Chen, Lei Zhang, Xiang Liu, Jiaqi Cheng, Yongjing Yan, Cuiping Zhang, Haihong Li
The combination of hair follicle-specific marker LHX2 and co-expressed marker can distinguish between sweat gland placodes and hair placodes in rat
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95. Yinxia Li, Xue Bai, Min Gao, Haikun Chen, Xiaoyao Ma, Yihan Zhang, Huhu Bai, Yanni Liu, Xiaodong Hu, Zhanwei Suo
AKAP150 and its Palmitoylation Contributed to Pain Hypersensitivity Via Facilitating Synaptic Incorporation of GluA1-Containing AMPA Receptor in Spinal Dorsal Horn
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96. Jiannan Du, Yuan Li, Yuting Su, Wenqian Zhi, Jiale Zhang, Cheng Zhang, Juan Wang, Wensheng Deng, Shasha Zhao
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97. Chenxi Zhao, Tiangang Zhou, Xiaoqing Zhao, Yilin Pang, Wenxiang Li, Baoyou Fan, Ming Li, Xinjie Liu, Lei Ma, Jiawei Zhang, Chao Sun, Wenyuan Shen, Xiaohong Kong, Xue Yao, Shiqing Feng
Delayed administration of nafamostat mesylate inhibits thrombin-mediated blood-spinal cord barrier breakdown during acute spinal cord injury in rats
J Neuroinflammation. 2022 Jul 16;19(1):189.;doi: 10.1186/s12974-022-02531-w.

98. Shi Mo, Wei Tang, Jun Xie, Sisi Chen, Luo Ren, Na Zang, Xiaohong Xie, Yu Deng, Leiqiong Gao, Enmei Liu
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99. Yanjie Guo, Ruofei Li, Xiaoqian Dang
S100A10 regulates tumor necrosis factor alpha-induced apoptosis in chondrocytes via the reactive oxygen species/nuclear factor-kappa B pathway
Biotechnol Appl Biochem. 2022 Dec;69(6):2284-2295.;doi: 10.1002/bab.2285.

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促销价: ¥ 487.00 470.00

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产品编号 产品名称 产品包装 产品价格
A0453 Alexa Fluor 555标记驴抗兔IgG(H+L) 100μl 487.00元

本Alexa Fluor 555标记驴抗兔IgG(H+L)(Alexa Fluor 555-labeled Donkey Anti-Rabbit IgG(H+L))为进口分装,用于免疫荧光染色。
Alexa Fluor 555是一种常用的非常明亮的红色荧光探针。它比绝大部分常用的红色荧光探针更加明亮,更加不容易淬灭,而且背景更低。Alexa Fluor 555的荧光光谱和Cy3非常接近。Alexa Fluor 555的吸收(激发)和发射峰参见下表。

Fluorophore Absorption Peak (nm) Emission Peak (nm)
Alexa Fluor 555 555 565

本抗体为用纯化的兔IgG免疫驴,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人IgG、小鼠IgG和大鼠IgG吸附纯化的优质二抗。
本Alexa Fluor 555标记驴抗兔IgG(H+L)用于免疫荧光染色时的推荐稀释比例为1:500。实际实验操作过程中需根据抗原和抗体的具体情况适当调节荧光标记二抗的稀释比例,推荐的调节范围为1:200-1000。
本抗体如果用于常规的免疫染色,以每次检测需1毫升1:500稀释的荧光标记二抗计,至少可以检测50次。如果适当重复使用已经使用过的荧光标记二抗,至少可以多检测150-250次。
包装清单:

产品编号 产品名称 包装
A0453 Alexa Fluor 555标记驴抗兔IgG (H+L) 100μl
说明书 1份

保存条件:
-20℃避光保存,一年有效。
注意事项:
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 免疫荧光染色请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
2. 如果希望重复使用稀释的荧光标记二抗,稀释的荧光标记二抗4℃保存。

Alexa Fluor 555标记驴抗兔IgG(H+L)(A0453)
Alexa Fluor 555标记驴抗兔IgG(H+L)(A0453)

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Alexa Fluor 555标记驴抗兔IgG(H+L)(A0453)

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Alexa Fluor 647标记山羊抗兔IgG(H+L)(A0468)

Alexa Fluor 647标记山羊抗兔IgG(H+L)
Alexa Fluor 647标记山羊抗兔IgG(H+L)(A0468) 一键复制产品信息

产品编号: A0468

产品包装:100μl
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100μl

说明书下载

565.00 545.00 10

价格: ¥ 565.00

促销价: ¥ 565.00

促销价: ¥ 565.00 545.00

产品简介
使用说明
产品文件
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产品编号 产品名称 产品包装 产品价格
A0468 Alexa Fluor 647标记山羊抗兔IgG(H+L) 100μl 565.00元

本Alexa Fluor 647标记山羊抗兔IgG(H+L)(Alexa Fluor 647-labeled Goat Anti-Rabbit IgG(H+L))为进口分装,用于免疫荧光染色。
Alexa Fluor 647是一种较常用的非常明亮的远红外荧光探针。通常该荧光探针被激发后肉眼不能观察到激发出来的长波长荧光,但可以被很多成像系统例如激光共聚焦显微镜等所检测到。Alexa Fluor 647的荧光光谱与Cy5比较接近。Alexa Fluor   647的吸收(激发)和发射峰参见下表。

Fluorophore Absorption Peak (nm) Emission Peak (nm)
Alexa Fluor 647 651 667

本抗体为用纯化的兔IgG免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人IgG、小鼠IgG和大鼠IgG吸附纯化的优质二抗。对人IgG、小鼠IgG和大鼠IgG几乎没有结合能力。特别适合于对于二抗种属特异性要求比较高的荧光染色实验。
本Alexa Fluor 647标记山羊抗兔IgG(H+L)用于免疫荧光染色时的推荐稀释比例为1:500。实际实验操作过程中需根据抗原和抗体的具体情况适当调节荧光标记二抗的稀释比例,推荐的调节范围为1:200-1000。
本抗体如果用于常规的免疫染色,以每次检测需1毫升1:500稀释的荧光标记二抗计,至少可以检测50次。如果适当重复使用已经使用过的荧光标记二抗,至少可以多检测150-250次。
包装清单:

产品编号 产品名称 包装
A0468 Alexa Fluor 647标记山羊抗兔IgG (H+L) 100μl
说明书 1份

保存条件:
-20℃避光保存,一年有效。
注意事项:
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 免疫荧光染色请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
2. 如果希望重复使用稀释的荧光标记二抗,稀释的荧光标记二抗4℃保存。

Alexa Fluor 647标记山羊抗兔IgG(H+L)(A0468)
Alexa Fluor 647标记山羊抗兔IgG(H+L)(A0468)

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Alexa Fluor 647标记山羊抗兔IgG(H+L)(A0468)

Alexa Fluor 647标记山羊抗兔IgG(H+L)(A0468)
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使用本产品的文献:

1. Jing Yan, Yuemei Zhang, Hairong Yu, Yicen Zong, Daixi Wang, Jiangfei Zheng, Li Jin, Xiangtian Yu, Caizhi Liu, Yi Zhang, Feng Jiang, Rong Zhang, Xiangnan Fang, Ting Xu, Mingyu Li, Jianzhong Di, Yan Lu, Xinran Ma, Jian Zhang, Weiping Jia, Cheng Hu
GPSM1 impairs metabolic homeostasis by controlling a pro-inflammatory pathway in macrophages
Nat Commun. 2022 Nov 25;13(1):7260.;doi: 10.1038/s41467-022-34998-9. (IF 12.121)

2. Liying Wang, Zimo Liu, Quan Zhou, Sufang Gu, Xiangsheng Liu, Jianxiang Huang, Haiping Jiang, Huifang Wang, Liping Cao, Jihong Sun, Youqing Shen, Huan Meng, Xiangrui Liu
Prodrug nanoparticles rationally integrating stroma modification and chemotherapy to treat metastatic pancreatic cancer
Biomaterials. 2021 Nov;278:121176.;doi: 10.1016/j.biomaterials.2021.121176. (IF 10.317)

3. Shuangqi Fan, Keke Wu, Mingqiu Zhao, Jin Yuan, Shengming Ma, Erpeng Zhu, Yuming Chen, Hongxing Ding, Lin Yi, Jinding Chen
LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection
Autophagy. 2021 Sep;17(9):2305-2324.;doi: 10.1080/15548627.2020.1823123. (IF 9.77)

4. Junfeng Zhang, Wei Xue, Kai Xu, Liang Yi, Yu Guo, Tian Xie, Haipeng Tong, Bo Zhou, Shunan Wang, Qing Li, Heng Liu, Xiao Chen, Jingqin Fang, Weiguo Zhang
Dual inhibition of PFKFB3 and VEGF normalizes tumor vasculature, reduces lactate production, and improves chemotherapy in glioblastoma: insights from protein expression profiling and MRI
Theranostics. 2020 Jun 5;10(16):7245-7259.;doi: 10.7150/thno.44427. (IF 8.579)

5. Zuo R, Wang Y, Li J, Wu J, Wang W, Li B, Sun C, Wang Z, Shi C, Zhou Y, Liu M, Zhang C
Rapamycin Induced Autophagy Inhibits Inflammation-Mediated Endplate Degeneration by Enhancing Nrf2/Keap1 Signaling of Cartilage Endplate Stem Cells.
Stem Cells. 2019 Jun;37(6):828-840. (IF 6.022)

6. Guan X, Zhang M, Fu M, Luo S, Hu Q
Herpes Simplex Virus Type 2 Immediate Early Protein ICP27 Inhibits IFN-β Production in Mucosal Epithelial Cells by Antagonizing IRF3 Activation.
Front Immunol. 2019 Feb 26;10:290. (IF 5.085)

7. Simin Shao, Haowei Cao, Zhongkun Wang, Dongmei Zhou, Chaoshen Wu, Shu Wang, Dian Xia, Daoyong Zhang
CHD4/NuRD complex regulates complement gene expression and correlates with CD8 T cell infiltration in human hepatocellular carcinoma
Clin Epigenetics. 2020 Feb 18;12(1):31.;doi: 10.1186/s13148-020-00827-3. (IF 5.028)

8. Ge C, Song J, Chen L, Wang L, Chen Y, Liu X, Zhang Y, Zhang L, Zhang M.
Atheroprotective pulsatile flow induces ubiquitin-proteasome-mediated degradation of programmed cell death 4 in endothelial cells.
PLoS One. 2014 Mar 13;9(3):e91564. (IF 2.74)

9. Lu J, Qian Y, Jin W, Tian R, Zhu Y, Wang J, Meng X, Wang R
Hypoxia-inducible factor-1α regulates epithelial-to-mesenchymal transition in paraquat-induced pulmonary fibrosis by activating lysyl oxidase.
Exp Ther Med. 2018 Mar;15(3):2287-2294. (IF 1.785)

10. Xiong D, Wu YB, Jin C, Li JJ, Gu J, Liao YF, Long X, Zhu SQ, Wu HB, Xu JJ, Ding JY
Elevated FUS/TLS expression is negatively associated with E-cadherin expression and prognosis of patients with non-small cell lung cancer.
Oncol Lett. 2018 Aug;16(2):1791-1800. (IF 2.311)

11. Wang G, Zhou H, Gu Z, Gao Q, Shen G
Oct4 promotes cancer cell proliferation and migration and leads to poor prognosis associated with the survivin/STAT3 pathway in hepatocellular carcinoma.
Oncol Rep. 2018 Aug;40(2):979-987. (IF 3.417)

12. Han X, Wu YC, Meng M, Sun QS, Gao SM, Sun H
Linarin prevents LPS‑induced acute lung injury by suppressing oxidative stress and inflammation via inhibition of TXNIP/NLRP3 and NF‑κB pathways.
Int J Mol Med. 2018 Sep;42(3):1460-1472. (IF 3.098)

13. Siyuan Chen, Li Yang, Hui Dong, Hong Guo
Human Telomerase Reverse Transcriptase Recruits the β-catenin/TCF-4 Complex to Transactivate Chemokine (C-C Motif) Ligand 2 Expression in Colorectal Cancer
Biomed Pharmacother. 2019 Apr;112:108700.;doi: 10.1016/j.biopha.2019.108700. (IF 4.545)

14. Zhang F, Zhu F, Yang J, Zhang W, Liu K, Ren L, Xiong F, Lu K, Li D, Zeng R, Wang X, Li J, Chen S, Wang Y, Chen J, Huang N
High-mobility group protein N2 induces autophagy by activating AMPK/ULK1 pathway and thereby boosts UPEC proliferation within bladder epithelial cells.
BIOCHEM BIOPH RES CO. 2019 May 21;513(1):193-200. (IF 2.985)

15. Qin L, Li M, Tan HL, Yang HX, Li SD, Luan ZX, Chen YF, Yang MH
Mechanistic target of rapamycin-mediated autophagy is involved in the alleviation of lipopolysaccharide-induced acute lung injury in rats.
Int Immunopharmacol. 2020 Jan;78:105790. (IF 3.943)

16. Shuangqi Fan, Keke Wu, Chaowei Luo, Xin Li, Mengpo Zhao, Dan Song, Shengming Ma, Erpeng Zhu, Yuming Chen, Hongxing Ding, Lin Yi, Jun Li, Mingqiu Zhao, Jinding Chen
Dual NDP52 Function in Persistent CSFV Infection
Front Microbiol. 2020 Jan 8;10:2962.;doi: 10.3389/fmicb.2019.02962. (IF 4.235)

17. Li Qin, Min Li, Hong-Ling Tan, Hong-Xing Yang, Shao-Dan Li, Zhen-Xian Luan, Ying-Fan Chen, Ming-Hui Yang
Mechanistic target of rapamycin-mediated autophagy is involved in the alleviation of lipopolysaccharide-induced acute lung injury in rats
Int Immunopharmacol. 2020 Jan;78:105790.;doi: 10.1016/j.intimp.2019.105790. (IF 3.943)

18. Bo Wang, Yan Wu, Zhuowang Ge, Xuan Zhang, Yexiang Yan, Yuquan Xie
NLRC5 deficiency ameliorates cardiac fibrosis in diabetic cardiomyopathy by regulating EndMT through Smad2/3 signaling pathway
BIOCHEM BIOPH RES CO. 2020 Jul 30;528(3):545-553.;doi: 10.1016/j.bbrc.2020.05.151. (IF 2.985)

19. Wenhui Li, Zilin Zhang, Liangliang Zhang, Hong Li, Shuangqi Fan, Erpeng Zhu, Jindai Fan, Zhaoyao Li, Wenxian Chen, Lin Yi, Hongxing Ding, Jinding Chen, Mingqiu Zhao
Antiviral Role of Serine Incorporator 5 (SERINC5) Proteins in Classical Swine Fever Virus Infection
Front Microbiol. 2020 Sep 4;11:580233.;doi: 10.3389/fmicb.2020.580233. (IF 4.235)

20. Jun Zhang, Tiantian Zuo, Jie Yang, Zongwei Hu, Zhihua Wang, Rui Xu, Siyu Ma, Yawen Wei, Qi Shen
Hierarchically Releasing Bio-Responsive Nanoparticles for Complete Tumor Microenvironment Modulation via TGF-β Pathway Inhibition and TAF Reduction
ACS Appl Mater Interfaces. 2021 Jan 20;13(2):2256-2268.;doi: 10.1021/acsami.0c18545.

21. Xing Liu, Fang Li, Jiwen Zhang, Lulu Wang, Jinliang Wang, Zhiyuan Wen, Zilong Wang, Lei Shuai, Xijun Wang, Jinying Ge, Dongming Zhao, Zhigao Bu
The ATPase ATP6V1A facilitates rabies virus replication by promoting virion uncoating and interacting with the viral matrix protein
J Biol Chem. 2021 Jan-Jun;296:100096.;doi: 10.1074/jbc.RA120.014190. (IF 4.238)

22. Wei Wang, Yu Zhong, Zhenkun Zhuang, Jiarui Xie, Yueer Lu, Chengzhi Huang, Yan Sun, Liang Wu, Jianhua Yin, Hang Yu, Zhiqiang Jiang, Shanshan Wang, Chunqing Wang, Yuanhang Zhang, Yilin Huang, Chongyin Han, Zhenggang Zhong, Jialin Hu, Ying Ouyang, Huisheng Liu, Mengya Yu, Xiaochan Wei, Dandan Chen, Lizhen Huang, Yong Hou, Zhanglin Lin, Shiping Liu, Fei Ling, Xueqing Yao
Multiregion single-cell sequencing reveals the transcriptional landscape of the immune microenvironment of colorectal cancer
Clin Transl Med. 2021 Jan;11(1):e253.;doi: 10.1002/ctm2.253.

23. Chao Yu, Dongdong Li, Chenggui Wang, Kaishun Xia, Jingkai Wang, Xiaopeng Zhou, Liwei Ying, Jiawei Shu, Xianpeng Huang, Haibin Xu, Bin Han, Qixin Chen, Fangcai Li, Jianbin Tang, Chengzhen Liang, Nigel Slater
Injectable kartogenin and apocynin loaded micelle enhances the alleviation of intervertebral disc degeneration by adipose-derived stem cell
Bioact Mater. 2021 Mar 23;6(10):3568-3579.;doi: 10.1016/j.bioactmat.2021.03.018.

24. Anqi Sun, Xiuhong Huang, Yanpeng Jiao, Xiangyu Wang, Jun Wen
Construction of biological factor-coated stent and its effect on promoting endothelialization
Mater Sci Eng C Mater Biol Appl. 2021 Mar;122:111943.;doi: 10.1016/j.msec.2021.111943.

25. Kefu Wu, Jiong Yue, Kaifeng Shen, Jiaojiang He, Gang Zhu, Shiyong Liu, Chunqing Zhang, Hui Yang
Increased expression of fibroblast growth factor 13 in cortical lesions of the focal cortical dysplasia
Brain Res Bull. 2021 Mar;168:36-44.;doi: 10.1016/j.brainresbull.2020.11.023. (IF 3.37)

26. Kefu Wu, Jiong Yue, Kaifeng Shen, Jiaojiang He, Gang Zhu, Shiyong Liu, Hui Yang, Chun-Qing Zhang
Expression and cellular distribution of FGF13 in cortical tubers of the tuberous sclerosis complex
Neurosci Lett. 2021 Apr 1;749:135714.;doi: 10.1016/j.neulet.2021.135714. (IF 2.274)

27. Feng Feng, Xueyan Feng, Di Zhang, Qilong Li, Li Yao
Matrix Stiffness Induces Pericyte-Fibroblast Transition Through YAP Activation
Front Pharmacol. 2021 May 31;12:698275.;doi: 10.3389/fphar.2021.698275. (IF 4.225)

28. Zifeng Zheng, Yuncheng Li, Mudan Zhang, Yalan Liu, Ming Fu, Sitang Gong, Qinxue Hu
Human Norovirus NTPase Antagonizes Interferon-β Production by Interacting With IkB Kinase ε
Front Microbiol. 2021 Jul 15;12:687933.;doi: 10.3389/fmicb.2021.687933. (IF 4.235)

29. Jing Qian, Jianyong Zhong, Shaojun Liu, Minhua Yan, Ping Cheng, Chuanming Hao, Yong Gu, Lingyun Lai
α-Klotho, Plasma Asymmetric Dimethylarginine, and Kidney Disease Progression
Kidney Med. 2021 Jul 29;3(6):984-991.e1.;doi: 10.1016/j.xkme.2021.05.008.

30. Zhongkuan Lyu, Yuanjin Chan, Qiyue Li, Qiang Zhang, Kaili Liu, Jun Xiang, Xiangting Li, Dingfang Cai, Yaming Li, Bing Wang, Zhonghai Yu
Destructive Effects of Pyroptosis on Homeostasis of Neuron Survival Associated with the Dysfunctional BBB-Glymphatic System and Amyloid-Beta Accumulation after Cerebral Ischemia/Reperfusion in Rats
Neural Plast. 2021 Aug 14;2021:4504363.;doi: 10.1155/2021/4504363. (IF 3.093)

31. Chengchen Wu, Junyi Zhao, Qiuping Wu, Qiulong Tan, Qiong Liu, Shifeng Xiao
Tau N-Terminal Inserts Regulate Tau Liquid-Liquid Phase Separation and Condensates Maturation in a Neuronal Cell Model
Int J Mol Sci. 2021 Sep 8;22(18):9728.;doi: 10.3390/ijms22189728. (IF 4.556)

32. Zhi-Qiang Hu, Qian Li, Zhen-Hong Hu, Hai-Chao Liu, Cheng-Long Rao, Mei-Juan Zhang, Yu-Pei Xia, Ling Deng, Xu-Hu Mao, Yao Fang
MicroRNA-146a inhibits autophagy to maintain the intracellular survival of Burkholderia pseudomallei by targeting LIPA
Microb Pathog. 2021 Sep;158:104969.;doi: 10.1016/j.micpath.2021.104969.

33. Qiong Zhu, Yi Zhang, Jiawei Tang, Najiao Tang, Ying He, Xiaoqin Chen, Shunji Gao, Yali Xu, Zheng Liu
Ultrasound-Targeted Microbubble Destruction Accelerates Angiogenesis and Ameliorates Left Ventricular Dysfunction after Myocardial Infarction in Mice
Ultrasound Med Biol. 2021 Sep;47(9):2692-2701.;doi: 10.1016/j.ultrasmedbio.2021.04.024. (IF 2.514)

34. Weiwei Liu, Liqiang Shi, Qiang Wan, Yansheng Wu, Di Huang, Jiaoying Ou, Qiuyu Liu, Xin Guan, Yuzhu Yang, Xiaotian Zhang, Jiandong Gao
Huangqi Guizhi Wuwu Decoction attenuates Podocyte cytoskeletal protein damage in IgA nephropathy rats by regulating AT1R/Nephrin/c-Abl pathway
Biomed Pharmacother. 2021 Oct;142:111907.;doi: 10.1016/j.biopha.2021.111907. (IF 4.545)

35. Yong Xu, Wenpan Peng, Di Han, Fanchao Feng, Zhichao Wang, Cheng Gu, Xianmei Zhou, Qi Wu
Maiwei Yangfei decoction prevents bleomycin-induced pulmonary fibrosis in mice
Exp Ther Med. 2021 Nov;22(5):1306.;doi: 10.3892/etm.2021.10741. (IF 1.785)

36. Ning Zhong, Wei Zhuang, Qian Huang, Qiang Wang, Wenjian Jin
Apatinib inhibits the growth of small cell lung cancer via a mechanism mediated by VEGF, PI3K/Akt and Ki-67/CD31
J Cell Mol Med. 2021 Nov;25(21):10039-10048.;doi: 10.1111/jcmm.16926. (IF 4.486)

37. Haokun Guo, Yan Liu, Juan Gu, Jing Luo, Yuanlin Ma, Fei Xiao
GJB1 mutations c.212T>G and c.311A>C induce apoptosis and inwardly rectifying potassium current changes in X-linked Charcot-Marie-Tooth type 1
Biochem Biophys Res Commun. 2021 Dec 10;582:8-15.;doi: 10.1016/j.bbrc.2021.10.029.

38. Siwen Zhang, Junfu Wang, Ting Chen, Jiancheng Wang, Ye Wang, Zhu Yu, Kun Zhao, Kaitian Zheng, Yeyang Chen, Zhen Wang, Bopei Li, Congjun Wang, Weijia Huang, Zhao Fu, Junqiang Chen
α-Actinin1 promotes tumorigenesis and epithelial-mesenchymal transition of gastric cancer via the AKT/GSK3β/β-Catenin pathway
Bioengineered. 2021 Dec;12(1):5688-5704.;doi: 10.1080/21655979.2021.1967713. (IF 2.205)

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Correction for Mo et al., “Respiratory Syncytial Virus Activates Rab5a to Suppress IRF1-Dependent Lambda Interferon Production, Subverting the Antiviral Defense of Airway Epithelial Cells”
J Virol. 2022 Sep 28;96(18):e0105422.;doi: 10.1128/jvi.01054-22. (IF 4.501)

Alexa Fluor 350标记山羊抗兔IgG(H+L)(A0408)

Alexa Fluor 350标记山羊抗兔IgG(H+L)
Alexa Fluor 350标记山羊抗兔IgG(H+L)(A0408) 一键复制产品信息

产品编号: A0408

产品包装:100μl
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100μl

说明书下载

529.00 511.00 10

价格: ¥ 529.00

促销价: ¥ 529.00

促销价: ¥ 529.00 511.00

产品简介
使用说明
产品文件
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产品编号 产品名称 产品包装 产品价格
A0408 Alexa Fluor 350标记山羊抗兔IgG(H+L) 100μl 529.00元

本Alexa Fluor 350标记山羊抗兔IgG (H+L)(Alexa Fluor 350-labeled Goat Anti-Rabbit IgG (H+L))为进口分装,用于免疫荧光染色。
Alexa Fluor 350是一种常用的非常明亮的蓝色荧光探针。Alexa Fluor 350的荧光光谱与DAPI和Hoechst比较接近。Alexa Fluor 350的吸收(激发)和发射峰参见下表。

Fluorophore Absorption Peak (nm) Emission Peak (nm)
Alexa Fluor 350 346 442

本抗体为用纯化的兔IgG免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人IgG、山羊IgG、牛IgG
(bovine IgG)、小鼠IgG和大鼠IgG吸附纯化的优质二抗。对人IgG、山羊IgG、牛IgG(bovine IgG)、小鼠IgG和大鼠IgG几乎没有结合能力。特别适合于对于二抗种属特异性要求比较高的荧光染色实验。

本Alexa Fluor 350标记山羊抗兔IgG(H+L)用于免疫荧光染色时的推荐稀释比例为1:500。实际实验操作过程中需根据抗原和抗体的具体情况适当调节荧光标记二抗的稀释比例,推荐的调节范围为1:200-1000。
本抗体如果用于常规的免疫染色,以每次检测需1毫升1:500稀释的荧光标记二抗计,至少可以检测50次。如果适当重复使用已经使用过的荧光标记二抗,至少可以多检测150-250次。
包装清单:

产品编号 产品名称 包装
A0408 Alexa Fluor 350标记山羊抗兔IgG (H+L) 100μl
说明书 1份

保存条件:
-20℃避光保存,一年有效。
注意事项:
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1. 免疫荧光染色请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
2. 如果希望重复使用稀释的荧光标记二抗,稀释的荧光标记二抗4℃保存。

Alexa Fluor 350标记山羊抗兔IgG(H+L)(A0408)
Alexa Fluor 350标记山羊抗兔IgG(H+L)(A0408)

物质安全数据单
输入右侧验证码点击下载:

Alexa Fluor 350标记山羊抗兔IgG(H+L)(A0408)

Alexa Fluor 350标记山羊抗兔IgG(H+L)(A0408)
质检证书
输入批号搜寻COA

搜索

相关产品请点击如下按钮:
荧光标记二抗

使用本产品的文献:

1. Wei Shi, Shuge Sun, Yu Han, Yu Tang, Weishang Zhou, Xueying Du, Guangxu Liu
Microplastics impair olfactory-mediated behaviors of goldfish Carassius auratus
J Hazard Mater. 2021 May 5;409:125016.;doi: 10.1016/j.jhazmat.2020.125016. (IF 9.038)

2. Hu T, Li SS, Lu MN, Zhang L, Chen B, Mao R, Mei R, Tan YX, Li S, Xiyang YB
Neuroprotection induced by Navβ2‑knockdown in APP/PS1 transgenic neurons is associated with NEP regulation.
Mol Med Rep. 2019 Aug;20(2):2002-2011. (IF 2.1)

3. Guo L, Li Y, Tian Y, Gong S, Chen X, Peng T, Wang A, Jiang Z
eIF2α promotes vascular remodeling via autophagy in monocrotaline-induced pulmonary arterial hypertension rats.
DRUG DES DEV THER. 2019 Aug 13;13:2799-2809. (IF 3.216)

4. Ding KK, Yang F, Jiang HQ, Yuan ZQ, Yin LL, Dong LY, Cui W, Gou Q, Liu XD, Wu YM, Jiang XY, Zhang X, Zhou PK, Yang CJ
Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells.
Oncol Lett. 2019 Sep;18(3):2704-2711. (IF 2.311)

5. Shangwen Xiao, Bin Song
LncRNA HOXA-AS2 promotes the progression of prostate cancer via targeting miR-509-3p/PBX3 axis
BIOSCIENCE REP. 2020 Aug 28;40(8):BSR20193287.;doi: 10.1042/BSR20193287. (IF 2.942)

6. Xinyu Bian, Dongmin Shi, Kailin Xing, Hongxin Zhou, Lili Lu, Dahai Yu, Weizhong Wu
AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation
Clin Transl Med. 2021 Mar;11(3):e352.;doi: 10.1002/ctm2.352.

7. Yu Yao, Dongxiao Qu, Xiaoping Jing, Yuxiang Jia, Qi Zhong, Limin Zhuo, Xingxing Chen, Guoyi Li, Lele Tang, Yudan Zhu, Xuemei Zhang, Yonghua Ji, Zhiping Li, Jie Tao
Molecular Mechanisms of Epileptic Encephalopathy Caused by KCNMA1 Loss-of-Function Mutations
Front Pharmacol. 2022 Jan 13;12:775328.;doi: 10.3389/fphar.2021.775328. (IF 4.225)

8. Kailu Wang, Pengfei Cui, Ruiqi Ni, Huiling Gong, Hao Li, Wenjun Yan, Xue Fu, Liang Chen, Changwei Lei, Hongning Wang, Xin Yang
Chicken-Derived Pattern Recognition Receptor chLGP2 Inhibits the Replication and Proliferation of Infectious Bronchitis Virus
Front Microbiol. 2022 Jan 25;12:810215.;doi: 10.3389/fmicb.2021.810215. (IF 4.235)

Alexa Fluor 647标记山羊抗小鼠IgG(H+L)(A0473)

Alexa Fluor 647标记山羊抗小鼠IgG(H+L)
Alexa Fluor 647标记山羊抗小鼠IgG(H+L)(A0473) 一键复制产品信息

产品编号: A0473

产品包装:100μl
选择包装

100μl

说明书下载

563.00 543.00 10

价格: ¥ 563.00

促销价: ¥ 563.00

促销价: ¥ 563.00 543.00

产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
A0473 Alexa Fluor 647标记山羊抗小鼠IgG(H+L) 100μl 563.00元

本Alexa Fluor 647标记山羊抗小鼠IgG(H+L)(Alexa Fluor 647-labeled Goat Anti-Mouse IgG(H+L))为进口分装,用于免疫荧光染色。
Alexa Fluor 647是一种较常用的非常明亮的远红外荧光探针。通常该荧光探针被激发后肉眼不能观察到激发出来的长波长荧光,但可以被很多成像系统例如激光共聚焦显微镜等所检测到。Alexa Fluor 647的荧光光谱与Cy5比较接近。Alexa Fluor   647的吸收(激发)和发射峰参见下表。

Fluorophore Absorption Peak (nm) Emission Peak (nm)
Alexa Fluor 647 651 667

本抗体为用纯化的小鼠IgG免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人IgG、马IgG、牛IgG(bovine IgG)、兔IgG和猪IgG吸附纯化的优质二抗。对人IgG、马IgG、牛IgG(bovine IgG)、兔IgG和猪IgG几乎没有结合能力。特别适合于对于二抗种属特异性要求比较高的荧光染色实验。
本Alexa Fluor 647标记山羊抗小鼠IgG(H+L)用于免疫荧光染色时的推荐稀释比例为1:500。实际实验操作过程中需根据抗原和抗体的具体情况适当调节荧光标记二抗的稀释比例,推荐的调节范围为1:200-1000。
本抗体如果用于常规的免疫染色,以每次检测需1毫升1:500稀释的荧光标记二抗计,至少可以检测50次。如果适当重复使用已经使用过的荧光标记二抗,至少可以多检测150-250次。
包装清单:

产品编号 产品名称 包装
A0473 Alexa Fluor 647标记山羊抗小鼠IgG (H+L) 100μl
说明书 1份

保存条件:
-20℃避光保存,一年有效。
注意事项:
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Alexa Fluor 647标记山羊抗小鼠IgG(H+L)(A0473)
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Alexa Fluor 647标记山羊抗小鼠IgG(H+L)(A0473)

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