标签归档：序列

pCMV-VSV-G 载体序列

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 pCMV-VSV-G 载体序列

型号 载体名称 出品公司 载体用途
VMA0369 pCMV-VSV-G Addgene 慢病毒载体
产品参数: 

5′ sequencing primer: T7

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

Vector for constitutive expression of VSV-G glycoprotein. Also known as pHCMV-G or pHCMV-VSV-G.

Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. pCMV-VSV-G expresses the G glycoprotein of the vesicular stomatitis virus （VSV-G） under the control of the CMV immediate-early promoter. VSV-G is used in pseudotyping of Moloney Murine Leukenia Virus（MMLV）-based retroviral vectors by mediating viral entry. VSV-G interacts with phospholipidcomponents of the target cell membrane and fosters the fusion of viral and cellular membranes. VSV-G does not require a cell surface receptor and can serve as a surrogate viral envelope protein. 
The vector contains the ampicillin-resistance gene for propagation and antibiotic selection in bacteria.

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pSicoR载体序列

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 pSicoR载体说明书

型号 载体名称 出品公司 载体用途
VMA0372 pSicoR Addgene 慢病毒载体
产品参数: 

Backbone size （bp）: 7567
Modifications to Backbone: EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5′: HpaI
Cloning site 3′: XhoI
5′ sequencing primer: mU6-F

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

pSicoR allows for conditional （Cre-Lox）, stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression.

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pCMV-VSV-G载体序列

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 pCMV-VSV-G载体序列说明书

型号 载体名称 出品公司 载体用途
VMA0369 pCMV-VSV-G Addgene 慢病毒载体
产品参数: 

5′ sequencing primer: T7

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

Vector for constitutive expression of VSV-G glycoprotein. Also known as pHCMV-G or pHCMV-VSV-G.

Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. pCMV-VSV-G expresses the G glycoprotein of the vesicular stomatitis virus （VSV-G） under the control of the CMV immediate-early promoter. VSV-G is used in pseudotyping of Moloney Murine Leukenia Virus（MMLV）-based retroviral vectors by mediating viral entry. VSV-G interacts with phospholipidcomponents of the target cell membrane and fosters the fusion of viral and cellular membranes. VSV-G does not require a cell surface receptor and can serve as a surrogate viral envelope protein. 
The vector contains the ampicillin-resistance gene for propagation and antibiotic selection in bacteria.

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pAdTrack 载体 序列及说明书

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pAdTrack

型号 载体名称 出品公司 载体用途
VXC0391 pAdTrack Coloncancer 腺病毒系统
产品参数: 

Size （bp）: 8320

载体抗性: 
卡那霉素（Kanamycin）
载体描述: 

Vector pAdTrack is used for expression of transgenes under a chosen （non-CMV） promoter when a GFP tracer is desired.

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pShuttle载体说明及序列

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pShuttle

型号 载体名称 出品公司 载体用途
VXS0389 pShuttle Stratagene 腺病毒系统
产品参数: 

Vector type: Mammalian Expression, Adenoviral
Growth strain（s）: DH10B
Growth temperature （℃）: 37
High or low copy: Low Copy

载体抗性: 
卡那霉素（Kanamycin）
载体描述: 

Vector pShuttle is used for expression of transgenes under a chosen （non-CMV） promoter when no GFP tracer is desired.

Max insert size = 7.5kb with pAdEasy®1 and 10.2kb with pAdEasy®2.

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pShuttle-CMV载体序列说明书

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pShuttle-CMV

型号 载体名称 出品公司 载体用途
VXS0388 pShuttle-CMV Stratagene 腺病毒系统
产品参数: 

Size （bp）: 7452
5′ sequencing primer: CMV-F

载体抗性: 
卡那霉素（Kanamycin）
载体描述: 

Vector pShuttle-CMV is used for expression of transgenes under a CMV promoter when no GFP tracer is desired.
Max insert size = 6.6kb with pAdEasy®1 and 9.3kb with pAdEasy®2.

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