SYBR Green I核酸凝胶染料 dsDNA荧光染料|SYBR Green I(10,000× in DMSO)

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SYBR Green I核酸凝胶染料 dsDNA荧光染料|SYBR Green I(10,000× in DMSO)

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产品简介

 

SYBR Green I，一种灵敏度非常高的dsDNA荧光染料，常用于核酸琼脂糖凝胶/聚丙烯酰胺凝胶电泳。SYBR Green I染料的最大激发波长为497 nm，但是在～290 nm和～380 nm处有二级激发峰。与DNA结合的SYBR Green I染料的荧光发射集中在520 nm。因此，该染料可适用于多种凝胶检测仪。

本品是溶于DMSO的SYBR Green I 10,000×染液。

 

产品信息

 

货号

10222ES60 / 10222ES76 / 10222ES86 / 10222ES90

规格

100 µL / 500 µL / 5×500 µL / 10×500 µL

 

储存条件

 

-25~-15℃保存，有效期1年。

 

使用说明

 

【开始使用前】

使用前将本品取出并回温至室温，使DMSO彻底解冻、溶液均一。于离心机上短暂离心确保所有溶液至管底。第一次使用时可将本品分装成多个小份后冻存，小份染料将更快速溶解。

  1. 染色

1）电泳后DNA染色

a. 在琼脂糖或非变性聚丙烯酰胺凝胶上进行电泳。

*TBE和TAE缓冲液均适合于SYBR Green I染料。

b. 用TE、TAE或TBE将SYBR Green I进行10,000倍稀释。

① SYBR Green I染色对pH敏感，为获得最佳的灵敏度，应确认在染色温度下染色液的pH值在7.5-8.0之间（pH8.0更佳）。

② 用水配制的染色液不如用缓冲液配制的稳定，为得到较好灵敏度，必须在24 h内使用。

c. 染液要充分覆盖凝胶，室温轻摇孵育10-40 min。

① 推荐用塑料而不是玻璃容器来储存染色液，因为染料会吸附到玻璃表面。

② 染色过程避光进行，建议在容器上加盖铝箔或置于暗处。

③ 凝胶厚度及琼脂糖或聚丙烯酰胺的比例不同，染色时间将有所不同。

④ 无需脱色。

⑤ 染液可置暗处（最好是冷藏）放置至少1周，重复使用最多4次。

2）电泳前DNA染色

a. 一般选择配制成SYBR Green I预制凝胶来实验。

临灌胶前将SYBR Green I储存液按照1：10,000倍稀释到凝胶溶液中，预制成含有SYBR Green I染料的琼脂糖或非变性聚丙烯酰胺凝胶。预制的SYBR Green I凝胶的DNA检测下限（大约为30-40 pg/条带），略高于电泳后染色（＜20 pg/条带）。此外，在SYBR Green I凝胶中的DNA片段迁移率明显比无染料凝胶中的相同片段慢。

b. 作为DNA模板预染标记。

一般而言，DNA在电泳前与染料工作液至少孵育15 min。

  1. 凝胶成像（紫外或蓝光透射仪或紫外顶置光源直射）

可在紫外或蓝光光源下轻松观察到用SYBR Green I染色的DNA。

  1. 从双链DNA中去除SYBR Green I

将SYBR Green I染色的DNA移至100 mM NaCl中，加入2.5倍体积的无水或95%的乙醇。在冰上孵育约20 min，在4℃离心至少10 min。去除乙醇，并用70%乙醇洗涤沉淀。让乙醇挥发，用TE重悬双链DNA即可。

 

注意事项

 

  1. 独立实验室进行的艾姆斯试验表明SYBR Green I 的致突变性明显比溴化乙锭要低得多，目前尚无关于SYBR Green I 染料对人体的致突变性或毒性的数据，但我们必须提醒用户注意，该染料能与DNA结合，因此，它应当被看作潜在诱变剂，在使用前小心谨慎。另外，在处理DMSO储存液时应特别小心，因为DMSO能促进有机分子进入组织。染料的处理应当符合当地的规定。
  2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
  3. 本产品仅作科研用途！

Ver.CN20240126

 

SYBR Green I核酸凝胶染料 dsDNA荧光染料|SYBR Green I(10,000× in DMSO)

暂无内容

[1] Li S, Huang S, Ke Y, et al. A HiPAD Integrated with rGO/MWCNTs Nano-Circuit Heater for Visual Point-of-Care Testing of SARS-CoV-2. Adv Funct Mater. 2021;31(26):2100801. doi:10.1002/adfm.202100801(IF:18.808)
[2] Ma N, Wang YK, Xu S, et al. PPDPF alleviates hepatic steatosis through inhibition of mTOR signaling. Nat Commun. 2021;12(1):3059. Published 2021 May 24. doi:10.1038/s41467-021-23285-8(IF:14.919)
[3] Lu W, Yu W, He J, et al. Reprogramming immunosuppressive myeloid cells facilitates immunotherapy for colorectal cancer. EMBO Mol Med. 2021;13(1):e12798. doi:10.15252/emmm.202012798(IF:12.137)
[4] Bai Z, Yang P, Yu F, et al. Combining adoptive NK cell infusion with a dopamine-releasing peptide reduces senescent cells in aged mice. Cell Death Dis. 2022;13(4):305. Published 2022 Apr 5. doi:10.1038/s41419-022-04562-w(IF:8.469)
[5] Wu X, Wang H, Zhu D, et al. USP3 promotes gastric cancer progression and metastasis by deubiquitination-dependent COL9A3/COL6A5 stabilisation. Cell Death Dis. 2021;13(1):10. Published 2021 Dec 20. doi:10.1038/s41419-021-04460-7(IF:8.469)
[6] Ahmad KA, Shoaib RM, Ahsan MZ, et al. Microglial IL-10 and β-endorphin expression mediates gabapentinoids antineuropathic pain. Brain Behav Immun. 2021;95:344-361. doi:10.1016/j.bbi.2021.04.007(IF:7.217)
[7] Liang Y, Kong Q, Luo H, Tan J, Zhu H. Exploring Long Non-Coding RNAs Associated with IP3/DAG Signaling Pathway as Potential Biomarkers Involved in Mast Cell Degranulation in Chronic Spontaneous Urticaria with 2-Year Follow-Up. J Inflamm Res. 2022;15:267-283. Published 2022 Jan 14. doi:10.2147/JIR.S343826(IF:6.922)
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[12] Zhang Y, Ma Y, Liu R, Li G. Genome-Wide Characterization and Expression Analysis of KH Family Genes Response to ABA and SA in Arabidopsis thaliana. Int J Mol Sci. 2022;23(1):511. Published 2022 Jan 3. doi:10.3390/ijms23010511(IF:5.924)
[13] Bi D, Zhu Y, Gao Y, et al. A newly developed PCR-based method revealed distinct Fusobacterium nucleatum subspecies infection patterns in colorectal cancer. Microb Biotechnol. 2021;14(5):2176-2186. doi:10.1111/1751-7915.13900(IF:5.813)
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[15] Zheng H, Liu H, Ge Y, Wang X. Integrated single-cell and bulk RNA sequencing analysis identifies a cancer associated fibroblast-related signature for predicting prognosis and therapeutic responses in colorectal cancer. Cancer Cell Int. 2021;21(1):552. Published 2021 Oct 20. doi:10.1186/s12935-021-02252-9(IF:5.722)
[16] Zhang H, Wang Q, Sun Q, et al. Effects of Compound 511 on BDNF-TrkB Signaling in the Mice Ventral Tegmental Area in Morphine-Induced Conditioned Place Preference. Cell Mol Neurobiol. 2021;41(5):961-975. doi:10.1007/s10571-020-00848-9(IF:5.046)
[17] Pan LL, Niu W, Fang X, et al. Clostridium butyricum Strains Suppress Experimental Acute Pancreatitis by Maintaining Intestinal Homeostasis [published online ahead of print, 2019 Apr 29]. Mol Nutr Food Res. 2019;e1801419. doi:10.1002/mnfr.201801419(IF:4.653)
[18] Yu HW, Lu ZH, Wang X, et al. Identification of a delayed leaf greening gene from a mutation of pummelo. Sci China Life Sci. 2021;64(7):1165-1173. doi:10.1007/s11427-020-1790-0(IF:4.611)
[19] Yang H, Yin F, Gan S, et al. The Study of Genetic Susceptibility and Mitochondrial Dysfunction in Mesial Temporal Lobe Epilepsy. Mol Neurobiol. 2020;57(9):3920-3930. doi:10.1007/s12035-020-01993-4(IF:4.500)
[20] Sun JN, Yu XY, Hou B, et al. Vaccarin enhances intestinal barrier function in type 2 diabetic mice. Eur J Pharmacol. 2021;908:174375. doi:10.1016/j.ejphar.2021.174375(IF:4.432)
[21] Chen L, Shi H, Wang X, et al. Hepatocyte nuclear factor 4 gamma (HNF4G) is correlated with poor prognosis and promotes tumor cell growth by inhibiting caspase-dependent intrinsic apoptosis in colorectal cancer. Eur J Pharmacol. 2022;916:174727. doi:10.1016/j.ejphar.2021.174727(IF:4.432)
[22] Xu Z, Zhou Y, Nong Q, et al. LKB1 Differently Regulates Adipogenesis in Intramuscular and Subcutaneous Adipocytes through Metabolic and Cytokine-Related Signaling Pathways. Cells. 2020;9(12):2599. Published 2020 Dec 4. doi:10.3390/cells9122599(IF:4.366)
[23] Jian M, Du Q, Zhu D, et al. Tumor suppressor miR-145-5p sensitizes prolactinoma to bromocriptine by downregulating TPT1. J Endocrinol Invest. 2019;42(6):639-652. doi:10.1007/s40618-018-0963-4(IF:4.256)
[24] Qin X, Zhang Y, Lu J, Huang S, Liu Z, Wang X. CYP3A deficiency alters bile acid homeostasis and leads to changes in hepatic susceptibility in rats. Toxicol Appl Pharmacol. 2021;429:115703. doi:10.1016/j.taap.2021.115703(IF:4.219)
[25] Sun X, Shu J, Ali Mohamed AM, et al. Identification and Characterization of EI (Elongated Internode) Gene in Tomato (Solanum lycopersicum). Int J Mol Sci. 2019;20(9):2204. Published 2019 May 5. doi:10.3390/ijms20092204(IF:4.183)
[26] Zhang F, Attarilar S, Xie K, et al. Carfilzomib alleviated osteoporosis by targeting PSME1/2 to activate Wnt/β-catenin signaling. Mol Cell Endocrinol. 2022;540:111520. doi:10.1016/j.mce.2021.111520(IF:4.102)
[27] Cheng ZY, Hu YH, Xia QP, Wang C, He L. DRD1 agonist A-68930 improves mitochondrial dysfunction and cognitive deficits in a streptozotocin-induced mouse model. Brain Res Bull. 2021;175:136-149. doi:10.1016/j.brainresbull.2021.07.015(IF:4.079)
[28] Yin X, Gao Y, Song S, Hassani D, Lu J. Identification, characterization and functional analysis of grape (Vitis vinifera L.) mitochondrial transcription termination factor (mTERF) genes in responding to biotic stress and exogenous phytohormone. BMC Genomics. 2021;22(1):136. Published 2021 Feb 26. doi:10.1186/s12864-021-07446-z(IF:3.969)
[29] Tian Y, Yuan X, Wang Y, et al. Soluble epoxide hydrolase inhibitor attenuates BBB disruption and neuroinflammation after intracerebral hemorrhage in mice. Neurochem Int. 2021;150:105197. doi:10.1016/j.neuint.2021.105197(IF:3.921)
[30] Chen Y, Ma X, Lou C, et al. PLA2G10 incorporated in exosomes could be diagnostic and prognostic biomarker for non-small cell lung cancer. Clin Chim Acta. 2022;530:55-65. doi:10.1016/j.cca.2022.02.016(IF:3.786)
[31] Bai J, Wei Q, Shu J, et al. Exploration of resistance to Phelipanche aegyptiaca in tomato. Pest Manag Sci. 2020;76(11):3806-3821. doi:10.1002/ps.5932(IF:3.750)
[32] Rao MJ, Xu Y, Huang Y, Tang X, Deng X, Xu Q. Ectopic expression of citrus UDP-GLUCOSYL TRANSFERASE gene enhances anthocyanin and proanthocyanidins contents and confers high light tolerance in Arabidopsis. BMC Plant Biol. 2019;19(1):603. Published 2019 Dec 30. doi:10.1186/s12870-019-2212-1(IF:3.670)
[33] Zhang XJ, Liu DM, Sun Y, et al. Potential risk of hyperuricemia: leading cardiomyocyte hypertrophy by inducing autophagy. Am J Transl Res. 2020;12(5):1894-1903. Published 2020 May 15. (IF:3.375)
[34] Zou C, Xu M, Chen L, et al. Xiaochaihu Decoction reduces hepatic steatosis and improves D-GalN/LPS-induced liver injury in hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀). Fish Shellfish Immunol. 2019;91:293-305. doi:10.1016/j.fsi.2019.05.025(IF:3.298)
[35] Wei Y, Zeng S, Zou C, et al. Porcine TRIM21 RING-finger E3 ubiquitin ligase is essential for anti-PRRSV activity. Vet Microbiol. 2021;256:109043. doi:10.1016/j.vetmic.2021.109043(IF:3.293)
[36] Jiang F, Hu X, Cao H, Shen X. Hsa_circ_0000081 promotes the function of gastric cancer through sponging hsa-miR-423-5p to influence 3-phosphoinositide-dependent kinase 1 expression. Bioengineered. 2022;13(4):8277-8290. doi:10.1080/21655979.2022.2053796(IF:3.269)
[37] Li Q, Li M, Zhu M, et al. Genome-wide identification and comparative analysis of Cry toxin receptor families in 7 insect species with a focus on Spodoptera litura. Insect Sci. 2022;29(3):783-800. doi:10.1111/1744-7917.12961(IF:3.262)
[38] Yang Z , Wu F , He Y , et al. A novel PTP1B inhibitor extracted from Ganoderma lucidum ameliorates insulin resistance by regulating IRS1-GLUT4 cascades in the insulin signaling pathway. Food Funct. 2018;9(1):397-406. doi:10.1039/c7fo01489a(IF:3.247)
[39] Qadeer A, Giri BR, Ullah H, Cheng G. Transcriptional profiles of genes potentially involved in extracellular vesicle biogenesis in Schistosoma japonicum. Acta Trop. 2021;217:105851. doi:10.1016/j.actatropica.2021.105851(IF:3.112)
[40] Yang Z, Zhang Z, Zhao J, He Y, Yang H, Zhou P. Modulation of energy metabolism and mitochondrial biogenesis by a novel proteoglycan from Ganoderma lucidum. RSC Adv. 2019;9(5):2591-2598. Published 2019 Jan 18. doi:10.1039/c8ra09482a(IF:3.049)
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[42] Yang Z, Chen C, Zhao J, et al. Hypoglycemic mechanism of a novel proteoglycan, extracted from Ganoderma lucidum, in hepatocytes. Eur J Pharmacol. 2018;820:77-85. doi:10.1016/j.ejphar.2017.12.020(IF:2.896)
[43] Ma H, Song X, Huang P, et al. Myricetin protects natural killer cells from arsenite induced DNA damage by attenuating oxidative stress and retaining poly(ADP-Ribose) polymerase 1 activity. Mutat Res Genet Toxicol Environ Mutagen. 2021;865:503337. doi:10.1016/j.mrgentox.2021.503337(IF:2.873)
[44] Xing W, Zhou D, Long Q, Sun M, Guo R, Wang L. Immune Response of Eastern Honeybee Worker to Nosema ceranae Infection Revealed by Transcriptomic Investigation. Insects. 2021;12(8):728. Published 2021 Aug 14. doi:10.3390/insects12080728(IF:2.769)
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[49] Xu H, Wang X, Wang W. Monomethylarsonous acid: Induction of DNA damage and oxidative stress in mouse natural killer cells at environmentally-relevant concentrations. Mutat Res Genet Toxicol Environ Mutagen. 2018;832-833:1-6. doi:10.1016/j.mrgentox.2018.05.017(IF:1.996)

产品简介

 

SYBR Green I，一种灵敏度非常高的dsDNA荧光染料，常用于核酸琼脂糖凝胶/聚丙烯酰胺凝胶电泳。SYBR Green I染料的最大激发波长为497 nm，但是在～290 nm和～380 nm处有二级激发峰。与DNA结合的SYBR Green I染料的荧光发射集中在520 nm。因此，该染料可适用于多种凝胶检测仪。

本品是溶于DMSO的SYBR Green I 10,000×染液。

 

产品信息

 

货号

10222ES60 / 10222ES76 / 10222ES86 / 10222ES90

规格

100 µL / 500 µL / 5×500 µL / 10×500 µL

 

储存条件

 

-25~-15℃保存，有效期1年。

 

使用说明

 

【开始使用前】

使用前将本品取出并回温至室温，使DMSO彻底解冻、溶液均一。于离心机上短暂离心确保所有溶液至管底。第一次使用时可将本品分装成多个小份后冻存，小份染料将更快速溶解。

  1. 染色

1）电泳后DNA染色

a. 在琼脂糖或非变性聚丙烯酰胺凝胶上进行电泳。

*TBE和TAE缓冲液均适合于SYBR Green I染料。

b. 用TE、TAE或TBE将SYBR Green I进行10,000倍稀释。

① SYBR Green I染色对pH敏感，为获得最佳的灵敏度，应确认在染色温度下染色液的pH值在7.5-8.0之间（pH8.0更佳）。

② 用水配制的染色液不如用缓冲液配制的稳定，为得到较好灵敏度，必须在24 h内使用。

c. 染液要充分覆盖凝胶，室温轻摇孵育10-40 min。

① 推荐用塑料而不是玻璃容器来储存染色液，因为染料会吸附到玻璃表面。

② 染色过程避光进行，建议在容器上加盖铝箔或置于暗处。

③ 凝胶厚度及琼脂糖或聚丙烯酰胺的比例不同，染色时间将有所不同。

④ 无需脱色。

⑤ 染液可置暗处（最好是冷藏）放置至少1周，重复使用最多4次。

2）电泳前DNA染色

a. 一般选择配制成SYBR Green I预制凝胶来实验。

临灌胶前将SYBR Green I储存液按照1：10,000倍稀释到凝胶溶液中，预制成含有SYBR Green I染料的琼脂糖或非变性聚丙烯酰胺凝胶。预制的SYBR Green I凝胶的DNA检测下限（大约为30-40 pg/条带），略高于电泳后染色（＜20 pg/条带）。此外，在SYBR Green I凝胶中的DNA片段迁移率明显比无染料凝胶中的相同片段慢。

b. 作为DNA模板预染标记。

一般而言，DNA在电泳前与染料工作液至少孵育15 min。

  1. 凝胶成像（紫外或蓝光透射仪或紫外顶置光源直射）

可在紫外或蓝光光源下轻松观察到用SYBR Green I染色的DNA。

  1. 从双链DNA中去除SYBR Green I

将SYBR Green I染色的DNA移至100 mM NaCl中，加入2.5倍体积的无水或95%的乙醇。在冰上孵育约20 min，在4℃离心至少10 min。去除乙醇，并用70%乙醇洗涤沉淀。让乙醇挥发，用TE重悬双链DNA即可。

 

注意事项

 

  1. 独立实验室进行的艾姆斯试验表明SYBR Green I 的致突变性明显比溴化乙锭要低得多，目前尚无关于SYBR Green I 染料对人体的致突变性或毒性的数据，但我们必须提醒用户注意，该染料能与DNA结合，因此，它应当被看作潜在诱变剂，在使用前小心谨慎。另外，在处理DMSO储存液时应特别小心，因为DMSO能促进有机分子进入组织。染料的处理应当符合当地的规定。
  2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
  3. 本产品仅作科研用途！

Ver.CN20240126

 

SYBR Green I核酸凝胶染料 dsDNA荧光染料|SYBR Green I(10,000× in DMSO)

暂无内容

[1] Li S, Huang S, Ke Y, et al. A HiPAD Integrated with rGO/MWCNTs Nano-Circuit Heater for Visual Point-of-Care Testing of SARS-CoV-2. Adv Funct Mater. 2021;31(26):2100801. doi:10.1002/adfm.202100801(IF:18.808)
[2] Ma N, Wang YK, Xu S, et al. PPDPF alleviates hepatic steatosis through inhibition of mTOR signaling. Nat Commun. 2021;12(1):3059. Published 2021 May 24. doi:10.1038/s41467-021-23285-8(IF:14.919)
[3] Lu W, Yu W, He J, et al. Reprogramming immunosuppressive myeloid cells facilitates immunotherapy for colorectal cancer. EMBO Mol Med. 2021;13(1):e12798. doi:10.15252/emmm.202012798(IF:12.137)
[4] Bai Z, Yang P, Yu F, et al. Combining adoptive NK cell infusion with a dopamine-releasing peptide reduces senescent cells in aged mice. Cell Death Dis. 2022;13(4):305. Published 2022 Apr 5. doi:10.1038/s41419-022-04562-w(IF:8.469)
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