TRIeasy™总RNA提取试剂|TRIeasy™ Total RNA Extraction Reagent

发表于由

TRIeasy™总RNA提取试剂|TRIeasy™ Total RNA Extraction Reagent

产品说明书

FAQ

COA

已发表文献

产品简介

TRIeasyTM Total RNA Extraction Reagent是一种适用于各种动植物、细菌组织、细胞的总RNA抽提试剂，具有极强的裂解能力，可在短时间内裂解细胞和组织样本，并有效抑制样本中RNA的降解，保持RNA的完整性。样品在该试剂中能够充分裂解，之后加入氯仿离心分层，形成上清层、中间层和有机层(鲜红色下层)，RNA分布在上层水相中，收集上清层后，经异丙醇沉淀便可得到总RNA。提取的总RNA纯度高，基本不含蛋白质及基因组DNA，可直接用于Northern、点杂交、mRNA纯化、体外翻译、RT-PCR、poly(A)+选择、RNA酶保护分析以及构建cDNA文库等多种分子生物学实验。

此外，样品中的DNA和蛋白也能以沉淀的方式还原。乙醇沉淀能析出中间层的DNA，而在有机层中加入异丙醇能沉淀出蛋白。本品操作简单快速，所有操作可在一小时内完成。对少量的组织(50-100 mg)和细胞(5×106)以及大量的组织(≥1 g)和细胞(>107)均有较好的裂解效果。

 

产品信息

货号

10606ES60

规格

100 mL

 

储存条件

2~8℃避光保存，有效期1年。

 

使用说明

表1 每毫升Total RNA Extraction Reagent能够充分裂解的最大样本量

样本类型

样本使用量

贴壁细胞

10 cm2培养面积

悬浮动植物细胞或酵母细胞

5×106-1×107个

细菌

107个

动物组织

50-100 mg

植物组织(多糖和多酚含量不高的)

50-100 mg

【注】：过多的样本量可能会导致裂解不充分，并使产物纯度降低。

 

注意事项

1. 本产品中含有苯酚，具有毒性和腐蚀性。如果吸入体内、接触皮肤、吞食等会引起中毒、灼伤及其他身体伤害。使用时应穿戴防护物，如防护服装、手套、眼罩、面罩等。如果不小心接触到眼睛时，应立即用大量的水冲洗并前往医院治疗。

2. 请穿实验服并佩戴一次性手套操作，避免RNase污染。

3. 需自备氯仿、异丙醇（新开封或提取RNA专用）、75%乙醇 (DEPC处理水配制)、DEPC和DEPC处理水。

4. 样品用Total RNA Extraction Reagent匀浆后，如果不加入氯仿进行下游实验，可先-70℃冻存，可保存一个月以上。

5. RNA沉淀在75%乙醇中，4℃可保存1周，-20℃可保存1年。

6. RNA半衰期比较短，易降解，建议抽提后尽快进行后续实验。

7. 本产品仅作科研用途！

 

Ver.CN20230809

Q：10606和19201的区别是什么？

A：19201是浓缩形式，需要的试剂用量更少一点，提取的准确性和提取反应的速度更快一些。一般10606就可以满足常规提取需求，如果客户想好更好的提取效果和rnase抑制效果可以用19201。

q: 用在贴壁细胞上总是不能完全从培养瓶（皿）脱落，是裂解不完全吗？

A: 这并不意味着裂解不完全。此时，细胞膜实际已完全裂开，并释放RNA，继续后续实验即可。

[1] Wang Y, Wang Y, Song D, et al. An RNA-cleaving threose nucleic acid enzyme capable of single point mutation discrimination. Nat Chem. 2022;14(3):350-359. doi:10.1038/s41557-021-00847-3(IF:24.427)
[2] Niu B, Liu J, Lv B, et al. Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis. Nat Commun. 2021;12(1):2809. Published 2021 May 14. doi:10.1038/s41467-021-23048-5(IF:14.919)
[3] Shui S, Zhao Z, Wang H, Conrad M, Liu G. Non-enzymatic lipid peroxidation initiated by photodynamic therapy drives a distinct ferroptosis-like cell death pathway. Redox Biol. 2021;45:102056. doi:10.1016/j.redox.2021.102056(IF:11.799)
[4] Fang Y, He Y, Wu C, et al. Magnetism-mediated targeting hyperthermia-immunotherapy in "cold" tumor with CSF1R inhibitor. Theranostics. 2021;11(14):6860-6872. Published 2021 May 3. doi:10.7150/thno.57511(IF:11.556)
[5] Jiang L, Yin X, Chen YH, et al. Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I. Theranostics. 2021;11(4):1703-1720. Published 2021 Jan 1. doi:10.7150/thno.43895(IF:11.556)
[6] Tang X, Deng Z, Ding P, et al. A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing. J Exp Clin Cancer Res. 2022;41(1):85. Published 2022 Mar 8. doi:10.1186/s13046-022-02276-7(IF:11.161)
[7] Zhou W, Li X, Wang Y, et al. Physiological and transcriptomic changes of zebrafish (Danio rerio) embryos-larvae in response to 2-MIB exposure. J Hazard Mater. 2021;416:126142. doi:10.1016/j.jhazmat.2021.126142(IF:10.588)
[8] Mo X, Du S, Chen X, et al. Lactate Induces Production of the tRNAHis Half to Promote B-lymphoblastic Cell Proliferation. Mol Ther. 2020;28(11):2442-2457. doi:10.1016/j.ymthe.2020.09.010(IF:8.986)
[9] Hao W, Han J, Chu Y, et al. Lower fluidity of supported lipid bilayers promotes neuronal differentiation of neural stem cells by enhancing focal adhesion formation. Biomaterials. 2018;161:106-116. doi:10.1016/j.biomaterials.2018.01.034(IF:8.806)
[10] Zou G, Zhang X, Wang L, et al. Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription. Theranostics. 2020;10(15):6839-6853. Published 2020 May 22. doi:10.7150/thno.43622(IF:8.579)
[11] Zhou X, Wang G, An X, et al. Polystyrene microplastic particles: In vivo and in vitro ocular surface toxicity assessment. Environ Pollut. 2022;303:119126. doi:10.1016/j.envpol.2022.119126(IF:8.071)
[12] Zhou W, Wang Y, Wang J, et al. β-Ionone causes endocrine disruption, hyperpigmentation and hypoactivity in zebrafish early life stages. Sci Total Environ. 2022;834:155433. doi:10.1016/j.scitotenv.2022.155433(IF:7.963)
[13] Zhao P, Zhang J, Wu A, et al. Biomimetic codelivery overcomes osimertinib-resistant NSCLC and brain metastasis via macrophage-mediated innate immunity. J Control Release. 2021;329:1249-1261. doi:10.1016/j.jconrel.2020.10.052(IF:7.727)
[14] Wu Z, Lu Z, Li L, et al. Identification and Validation of Ferroptosis-Related LncRNA Signatures as a Novel Prognostic Model for Colon Cancer. Front Immunol. 2022;12:783362. Published 2022 Jan 26. doi:10.3389/fimmu.2021.783362(IF:7.561)
[15] Lv Y, Wang X, Li X, et al. Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway. PLoS Biol. 2020;18(11):e3000872. Published 2020 Nov 13. doi:10.1371/journal.pbio.3000872(IF:7.076)
[16] Guo Q, Wang T, Yang Y, et al. Transcriptional Factor Yin Yang 1 Promotes the Stemness of Breast Cancer Cells by Suppressing miR-873-5p Transcriptional Activity. Mol Ther Nucleic Acids. 2020;21:527-541. doi:10.1016/j.omtn.2020.06.018(IF:7.032)
[17] Duan Y, Jiang N, Chen J, Chen J. Expression, localization and metabolic function of "resurrected" human urate oxidase in human hepatocytes. Int J Biol Macromol. 2021;175:30-39. doi:10.1016/j.ijbiomac.2021.01.163(IF:6.953)
[18] Liu X, Wang C, Pang L, Pan L, Zhang Q. Combination of resolvin E1 and lipoxin A4 promotes the resolution of pulpitis by inhibiting NF-κB activation through upregulating sirtuin 7 in dental pulp fibroblasts. Cell Prolif. 2022;55(5):e13227. doi:10.1111/cpr.13227(IF:6.831)
[19] Luo X, Sun X, Wang Y, et al. Clinical Study of 8 Cases of CHD2 Gene Mutation-Related Neurological Diseases and Their Mechanisms. Front Cell Dev Biol. 2022;10:853127. Published 2022 Mar 21. doi:10.3389/fcell.2022.853127(IF:6.684)
[20] Wang Y, Zhao M, Li W, et al. BMSC-Derived Small Extracellular Vesicles Induce Cartilage Reconstruction of Temporomandibular Joint Osteoarthritis via Autotaxin-YAP Signaling Axis. Front Cell Dev Biol. 2021;9:656153. Published 2021 Apr 1. doi:10.3389/fcell.2021.656153(IF:6.684)
[21] Han S, Cao D, Sha J, Zhu X, Chen D. LncRNA ZFPM2-AS1 promotes lung adenocarcinoma progression by interacting with UPF1 to destabilize ZFPM2. Mol Oncol. 2020;14(5):1074-1088. doi:10.1002/1878-0261.12631(IF:6.574)
[22] Wang J, Hu R, Wang Z, et al. Establishment of Immortalized Yak Ruminal Epithelial Cell Lines by Lentivirus-Mediated SV40T and hTERT Gene Transduction. Oxid Med Cell Longev. 2022;2022:8128028. Published 2022 Mar 25. doi:10.1155/2022/8128028(IF:6.543)
[23] Fan H, Wang S, Wang H, Sun M, Wu S, Bao W. Melatonin Ameliorates the Toxicity Induced by Deoxynivalenol in Murine Ovary Granulosa Cells by Antioxidative and Anti-Inflammatory Effects. Antioxidants (Basel). 2021;10(7):1045. Published 2021 Jun 29. doi:10.3390/antiox10071045(IF:6.313)
[24] Wang Y, Du S, Zhu C, et al. STUB1 is targeted by the SUMO-interacting motif of EBNA1 to maintain Epstein-Barr Virus latency. PLoS Pathog. 2020;16(3):e1008447. Published 2020 Mar 16. doi:10.1371/journal.ppat.1008447(IF:6.218)
[25] Li A, Liu Q, Li Q, Liu B, Yang Y, Zhang N. Berberine Reduces Pyruvate-driven Hepatic Glucose Production by Limiting Mitochondrial Import of Pyruvate through Mitochondrial Pyruvate Carrier 1. EBioMedicine. 2018;34:243-255. doi:10.1016/j.ebiom.2018.07.039(IF:6.183)
[26] Chen LS, Zhang M, Chen P, et al. The m6A demethylase FTO promotes the osteogenesis of mesenchymal stem cells by downregulating PPARG. Acta Pharmacol Sin. 2022;43(5):1311-1323. doi:10.1038/s41401-021-00756-8(IF:6.150)
[27] Xue H, Lu J, Yan H, et al. γ-Glutamyl transpeptidase-activated indole-quinolinium based cyanine as a fluorescence turn-on nucleolus-targeting probe for cancer cell detection and inhibition. Talanta. 2022;237:122898. doi:10.1016/j.talanta.2021.122898(IF:6.057)
[28] Yang N, Zhang X, Li L, et al. Ginsenoside Rc Promotes Bone Formation in Ovariectomy-Induced Osteoporosis In Vivo and Osteogenic Differentiation In Vitro. Int J Mol Sci. 2022;23(11):6187. Published 2022 May 31. doi:10.3390/ijms23116187(IF:5.924)
[29] Song J, Meng Y, Wang M, et al. Mangiferin activates Nrf2 to attenuate cardiac fibrosis via redistributing glutaminolysis-derived glutamate. Pharmacol Res. 2020;157:104845. doi:10.1016/j.phrs.2020.104845(IF:5.893)
[30] Zhenzhen L, Wenting L, Jianmin Z, et al. miR-146a-5p/TXNIP axis attenuates intestinal ischemia-reperfusion injury by inhibiting autophagy via the PRKAA/mTOR signaling pathway. Biochem Pharmacol. 2022;197:114839. doi:10.1016/j.bcp.2021.114839(IF:5.858)
[31] Yan H, Lu J, Wang J, et al. Prevention of Cyclophosphamide-Induced Immunosuppression in Mice With Traditional Chinese Medicine Xuanfei Baidu Decoction [published correction appears in Front Pharmacol. 2022 Jan 20;12:808424]. Front Pharmacol. 2021;12:730567. Published 2021 Oct 19. doi:10.3389/fphar.2021.730567(IF:5.811)
[32] Wei W, Cao T, Pathak JL, et al. Apigenin, a Single Active Component of Herbal Extract, Alleviates Xerostomia via ERα-Mediated Upregulation of AQP5 Activation. Front Pharmacol. 2022;13:818116. Published 2022 Feb 21. doi:10.3389/fphar.2022.818116(IF:5.811)
[33] Chen Y, Chen J, Shu A, et al. Combination of the Herbs Radix Rehmanniae and Cornus Officinalis Mitigated Testicular Damage From Diabetes Mellitus by Enhancing Glycolysis via the AGEs/RAGE/HIF-1α Axis. Front Pharmacol. 2021;12:678300. Published 2021 Jun 28. doi:10.3389/fphar.2021.678300(IF:5.811)
[34] Cao L, Lu X, Wang G, et al. Maize ZmbZIP33 Is Involved in Drought Resistance and Recovery Ability Through an Abscisic Acid-Dependent Signaling Pathway. Front Plant Sci. 2021;12:629903. Published 2021 Apr 1. doi:10.3389/fpls.2021.629903(IF:5.754)
[35] Cheng H, Fan X, Wang X, et al. Hierarchically Self-Assembled Supramolecular Host-Guest Delivery System for Drug Resistant Cancer Therapy. Biomacromolecules. 2018;19(6):1926-1938. doi:10.1021/acs.biomac.7b01693(IF:5.738)
[36] Bao L, Yuan L, Li P, et al. A FUS-LATS1/2 Axis Inhibits Hepatocellular Carcinoma Progression via Activating Hippo Pathway. Cell Physiol Biochem. 2018;50(2):437-451. doi:10.1159/000494155(IF:5.500)
[37] Wang J, Li HY, Wang HS, Su ZB. MicroRNA-485 Modulates the TGF-β/ Smads Signaling Pathway in Chronic Asthmatic Mice by Targeting Smurf2 [retracted in: Cell Physiol Biochem. 2021;55(5):658]. Cell Physiol Biochem. 2018;51(2):692-710. doi:10.1159/000495327(IF:5.500)
[38] Li J, Yang YL, Li LZ, et al. Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: Therapeutic effects of ginsenoside Rb1. Biochim Biophys Acta Mol Basis Dis. 2017;1863(11):2835-2847. doi:10.1016/j.bbadis.2017.07.017(IF:5.476)
[39] Fan X, Cheng H, Wang X, et al. Thermoresponsive Supramolecular Chemotherapy by "V"-Shaped Armed β-Cyclodextrin Star Polymer to Overcome Drug Resistance. Adv Healthc Mater. 2018;7(7):e1701143. doi:10.1002/adhm.201701143(IF:5.110)
[40] Li C, Zhu Y, Wu Y, et al. Oridonin Alleviates LPS-Induced Depression by Inhibiting NLRP3 Inflammasome via Activation of Autophagy. Front Med (Lausanne). 2022;8:813047. Published 2022 Jan 12. doi:10.3389/fmed.2021.813047(IF:5.093)
[41] He ZD, Zhang M, Wang YH, et al. Anti-PD-L1 mediating tumor-targeted codelivery of liposomal irinotecan/JQ1 for chemo-immunotherapy. Acta Pharmacol Sin. 2021;42(9):1516-1523. doi:10.1038/s41401-020-00570-8(IF:5.064)
[42] Liu Y, Chen D, Zhang X, et al. Construction of Baculovirus-Inducible CRISPR/Cas9 Antiviral System Targeting BmNPV in Bombyx mori. Viruses. 2021;14(1):59. Published 2021 Dec 30. doi:10.3390/v14010059(IF:5.048)
[43] Hu R, Zhu X, Chen C, Xu R, Li Y, Xu W. RNA-binding protein PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3'UTR with miRNAs. Cell Prolif. 2018;51(6):e12508. doi:10.1111/cpr.12508(IF:4.936)
[44] Li Z, Wang Y, Hu R, Xu R, Xu W. LncRNA B4GALT1-AS1 recruits HuR to promote osteosarcoma cells stemness and migration via enhancing YAP transcriptional activity. Cell Prolif. 2018;51(6):e12504. doi:10.1111/cpr.12504(IF:4.936)
[45] Wei R, Zhong S, Qiao L, et al. Steroid 5α-Reductase Type I Induces Cell Viability and Migration via Nuclear Factor-κB/Vascular Endothelial Growth Factor Signaling Pathway in Colorectal Cancer. Front Oncol. 2020;10:1501. Published 2020 Aug 28. doi:10.3389/fonc.2020.01501(IF:4.848)
[46] Wang S, He X, Li Q, et al. Obstructive Sleep Apnea Affects Lacrimal Gland Function. Invest Ophthalmol Vis Sci. 2022;63(3):3. doi:10.1167/iovs.63.3.3(IF:4.799)
[47] Zhou X , Weng W , Chen B , et al. Mesoporous silica nanoparticles/gelatin porous composite scaffolds with localized and sustained release of vancomycin for treatment of infected bone defects. J Mater Chem B. 2018;6(5):740-752. doi:10.1039/c7tb01246b(IF:4.776)
[48] Li Z, Liu S, Fu T, Peng Y, Zhang J. Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells. Stem Cell Res Ther. 2019;10(1):351. Published 2019 Nov 27. doi:10.1186/s13287-019-1441-4(IF:4.627)
[49] Wang J, Ren D, Sun Y, et al. Inhibition of PLK4 might enhance the anti-tumour effect of bortezomib on glioblastoma via PTEN/PI3K/AKT/mTOR signalling pathway. J Cell Mol Med. 2020;24(7):3931-3947. doi:10.1111/jcmm.14996(IF:4.486)
[50] Karuppiah V, Vallikkannu M, Li T, Chen J. Simultaneous and sequential based co-fermentations of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841: a strategy to enhance the gene expression and metabolites to improve the bio-control and plant growth promoting activity. Microb Cell Fact. 2019;18(1):185. Published 2019 Oct 29. doi:10.1186/s12934-019-1233-7(IF:4.402)
[51] Wu Y, Min L, Xu Y, et al. Combination of molecular docking and liver transcription sequencing analysis for the evaluation of salt-processed psoraleae fructus-induced hepatotoxicity in ovariectomized mice. J Ethnopharmacol. 2022;288:114955. doi:10.1016/j.jep.2021.114955(IF:4.360)
[52] Karuppiah V, Sun J, Li T, Vallikkannu M, Chen J. Co-cultivation of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841 Causes Differential Gene Expression and Improvement in the Wheat Growth and Biocontrol Activity. Front Microbiol. 2019;10:1068. Published 2019 May 16. doi:10.3389/fmicb.2019.01068(IF:4.259)
[53] Tang H, Yu Q, Li Z, et al. A PIP-mediated osmotic stress signaling cascade plays a positive role in the salt tolerance of sugarcane. BMC Plant Biol. 2021;21(1):589. Published 2021 Dec 13. doi:10.1186/s12870-021-03369-9(IF:4.215)
[54] Zhu W, Hu J, Li Y, et al. Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca2+ in the Primordium Differentiation. Int J Mol Sci. 2019;20(24):6317. Published 2019 Dec 14. doi:10.3390/ijms20246317(IF:4.183)
[55] Yin X, Wei Y, Song W, et al. Melatonin as an inducer of arecoline and their coordinated roles in anti-oxidative activity and immune responses. Food Funct. 2020;11(10):8788-8799. doi:10.1039/d0fo01841d(IF:4.171)
[56] Sun T, Zhang L, Feng J, et al. Characterization of cellular senescence in doxorubicin-induced aging mice. Exp Gerontol. 2022;163:111800. doi:10.1016/j.exger.2022.111800(IF:4.032)
[57] Wang W, Wang L, Wang L, et al. Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencing. BMC Genomics. 2021;22(1):109. Published 2021 Feb 9. doi:10.1186/s12864-021-07416-5(IF:3.969)
[58] Qiu BQ, Zhang PF, Xiong D, et al. CircRNA fibroblast growth factor receptor 3 promotes tumor progression in non-small cell lung cancer by regulating Galectin-1-AKT/ERK1/2 signaling. J Cell Physiol. 2019;234(7):11256-11264. doi:10.1002/jcp.27783(IF:3.923)
[59] Xu J, Luo X, Fang G, et al. Transgenic expression of antimicrobial peptides from black soldier fly enhance resistance against entomopathogenic bacteria in the silkworm, Bombyx mori. Insect Biochem Mol Biol. 2020;127:103487. doi:10.1016/j.ibmb.2020.103487(IF:3.827)
[60] Liao Y, Zhang H, He D, et al. Retinal Pigment Epithelium Cell Death Is Associated With NLRP3 Inflammasome Activation by All-trans Retinal. Invest Ophthalmol Vis Sci. 2019;60(8):3034-3045. doi:10.1167/iovs.18-26360(IF:3.812)
[61] Li Z, Zhang X, Yuan T, et al. Addition of Platelet-Rich Plasma to Silk Fibroin Hydrogel Bioprinting for Cartilage Regeneration. Tissue Eng Part A. 2020;26(15-16):886-895. doi:10.1089/ten.TEA.2019.0304(IF:3.776)
[62] Wang C, Wang Y, Wang C, et al. Therapeutic application of 3B-PEG injectable hydrogel/Nell-1 composite system to temporomandibular joint osteoarthritis. Biomed Mater. 2021;17(1):10.1088/1748-605X/ac367f. Published 2021 Nov 19. doi:10.1088/1748-605X/ac367f(IF:3.715)
[63] Wang M, Tao H, Huang P. Clinical significance of LARGE1 in progression of liver cancer and the underlying mechanism. Gene. 2021;779:145493. doi:10.1016/j.gene.2021.145493(IF:3.688)
[64] Tong X, Wang Y, Sun A, Bello BK, Ni S, Zhang J. Notched Belly Grain 4, a Novel Allele of Dwarf 11, Regulates Grain Shape and Seed Germination in Rice (Oryza sativa L.). Int J Mol Sci. 2018;19(12):4069. Published 2018 Dec 16. doi:10.3390/ijms19124069(IF:3.687)
[65] Tong X, Wang Y, Sun A, Bello BK, Ni S, Zhang J. Notched Belly Grain 4, a Novel Allele of Dwarf 11, Regulates Grain Shape and Seed Germination in Rice (Oryza sativa L.). Int J Mol Sci. 2018;19(12):4069. Published 2018 Dec 16. doi:10.3390/ijms19124069(IF:3.687)
[66] Zhu Y, Du Q, Jiao N, et al. Catalpol ameliorates diabetes-induced testicular injury and modulates gut microbiota. Life Sci. 2021;267:118881. doi:10.1016/j.lfs.2020.118881(IF:3.647)
[67] Chen L, Qin L, Chen C, Hu Q, Wang J, Shen J. Serum exosomes accelerate diabetic wound healing by promoting angiogenesis and ECM formation. Cell Biol Int. 2021;45(9):1976-1985. doi:10.1002/cbin.11627(IF:3.612)
[68] Cai Y, Liu Y, Sun Y, Ren Y. Mesenchyme homeobox 2 has a cancer-inhibiting function in breast carcinoma via affection of the PI3K/AKT/mTOR and ERK1/2 pathways. Biochem Biophys Res Commun. 2022;593:20-27. doi:10.1016/j.bbrc.2022.01.011(IF:3.575)
[69] Yang Y, Wang L, Che Z, et al. Novel target sites for soybean yield enhancement by photosynthesis. J Plant Physiol. 2022;268:153580. doi:10.1016/j.jplph.2021.153580(IF:3.549)
[70] Qiao S, Li B, Cai Q, et al. Involvement of ferroptosis in Porphyromonas gingivalis lipopolysaccharide-stimulated periodontitis in vitro and in vivo [published online ahead of print, 2022 Jun 28]. Oral Dis. 2022;10.1111/odi.14292. doi:10.1111/odi.14292(IF:3.511)
[71] Zhang Y, Niu J, Zhang S, et al. Comparative study on the gastrointestinal- and immune- regulation functions of Hedysari Radix Paeparata Cum Melle and Astragali Radix Praeparata cum Melle in rats with spleen-qi deficiency, based on fuzzy matter-element analysis. Pharm Biol. 2022;60(1):1237-1254. doi:10.1080/13880209.2022.2086990(IF:3.503)
[72] Song H, Xu Y, Shi L, et al. LncRNA THOR increases the stemness of gastric cancer cells via enhancing SOX9 mRNA stability. Biomed Pharmacother. 2018;108:338-346. doi:10.1016/j.biopha.2018.09.057(IF:3.457)
[73] Zhang M, Li Y, Wang H, Yu W, Lin S, Guo J. LncRNA SNHG5 affects cell proliferation, metastasis and migration of colorectal cancer through regulating miR-132-3p/CREB5. Cancer Biol Ther. 2019;20(4):524-536. doi:10.1080/15384047.2018.1537579(IF:3.373)
[74] Ding Q, Sun J, Xie W, Zhang M, Zhang C, Xu X. Stemona alkaloids suppress the positive feedback loop between M2 polarization and fibroblast differentiation by inhibiting JAK2/STAT3 pathway in fibroblasts and CXCR4/PI3K/AKT1 pathway in macrophages. Int Immunopharmacol. 2019;72:385-394. doi:10.1016/j.intimp.2019.04.030(IF:3.361)
[75] Hou J, Qian J, Li Z, et al. Bioactive Compounds from Abelmoschus manihot L. Alleviate the Progression of Multiple Myeloma in Mouse Model and Improve Bone Marrow Microenvironment. Onco Targets Ther. 2020;13:959-973. Published 2020 Jan 31. doi:10.2147/OTT.S235944(IF:3.337)
[76] Ni S, Li Z, Ying J, Zhang J, Chen H. Decreased Spikelets 4 Encoding a Novel Tetratricopeptide Repeat Domain-Containing Protein Is Involved in DNA Repair and Spikelet Number Determination in Rice. Genes (Basel). 2019;10(3):214. Published 2019 Mar 13. doi:10.3390/genes10030214(IF:3.331)
[77] Chen S, Li M, Jiang W, Zheng H, Qi LW, Jiang S. The role of Neu1 in the protective effect of dipsacoside B on acetaminophen-induced liver injury. Ann Transl Med. 2020;8(13):823. doi:10.21037/atm-19-3850(IF:3.297)
[78] Cao L, Lu X, Wang G, et al. Transcriptional regulatory networks in response to drought stress and rewatering in maize (Zea mays L.). Mol Genet Genomics. 2021;296(6):1203-1219. doi:10.1007/s00438-021-01820-y(IF:3.291)
[79] Yang Z , Wu F , He Y , et al. A novel PTP1B inhibitor extracted from Ganoderma lucidum ameliorates insulin resistance by regulating IRS1-GLUT4 cascades in the insulin signaling pathway. Food Funct. 2018;9(1):397-406. doi:10.1039/c7fo01489a(IF:3.247)
[80] Li YY, Cai Q, Li BS, et al. The Effect of Porphyromonas gingivalis Lipopolysaccharide on the Pyroptosis of Gingival Fibroblasts. Inflammation. 2021;44(3):846-858. doi:10.1007/s10753-020-01379-7(IF:3.212)
[81] Gong F, Dong D, Zhang T, Xu W. Long non-coding RNA FENDRR attenuates the stemness of non-small cell lung cancer cells via decreasing multidrug resistance gene 1 (MDR1) expression through competitively binding with RNA binding protein HuR. Eur J Pharmacol. 2019;853:345-352. doi:10.1016/j.ejphar.2019.04.022(IF:3.170)
[82] Song H, Shi L, Xu Y, et al. BRD4 promotes the stemness of gastric cancer cells via attenuating miR-216a-3p-mediated inhibition of Wnt/β-catenin signaling. Eur J Pharmacol. 2019;852:189-197. doi:10.1016/j.ejphar.2019.03.018(IF:3.170)
[83] Tan JK, Ma XF, Wang GN, Jiang CR, Gong HQ, Liu H. LncRNA MIAT knockdown alleviates oxygen-glucose deprivation‑induced cardiomyocyte injury by regulating JAK2/STAT3 pathway via miR-181a-5p. J Cardiol. 2021;78(6):586-597. doi:10.1016/j.jjcc.2021.08.018(IF:3.159)
[84] Tan Q, Lin S, Zeng Y, et al. Ginsenoside Rg3 attenuates the osimertinib resistance by reducing the stemness of non-small cell lung cancer cells. Environ Toxicol. 2020;35(6):643-651. doi:10.1002/tox.22899(IF:3.118)
[85] Yang Z, Zhang Z, Zhao J, He Y, Yang H, Zhou P. Modulation of energy metabolism and mitochondrial biogenesis by a novel proteoglycan from Ganoderma lucidum. RSC Adv. 2019;9(5):2591-2598. Published 2019 Jan 18. doi:10.1039/c8ra09482a(IF:3.049)
[86] Xu L, Sheng T, Liu X, Zhang T, Wang Z, Han H. Analyzing the hepatoprotective effect of the Swertia cincta Burkillextract against ANIT-induced cholestasis in rats by modulating the expression of transporters and metabolic enzymes. J Ethnopharmacol. 2017;209:91-99. doi:10.1016/j.jep.2017.07.031(IF:2.981)
[87] Wang S, Zhang G, Zheng W, et al. MiR-454-3p and miR-374b-5p suppress migration and invasion of bladder cancer cells through targetting ZEB2. Biosci Rep. 2018;38(6):BSR20181436. Published 2018 Dec 7. doi:10.1042/BSR20181436(IF:2.899)
[88] Yang Z, Chen C, Zhao J, et al. Hypoglycemic mechanism of a novel proteoglycan, extracted from Ganoderma lucidum, in hepatocytes. Eur J Pharmacol. 2018;820:77-85. doi:10.1016/j.ejphar.2017.12.020(IF:2.896)
[89] Liu Z, Li J, Hu X, Xu H. Helicobacter pylori-induced protein tyrosine phosphatase receptor type C as a prognostic biomarker for gastric cancer. J Gastrointest Oncol. 2021;12(3):1058-1073. doi:10.21037/jgo-21-305(IF:2.892)
[90] Zeng Y, Shi XB, Yuan ZY, et al. Biological characteristics of renal cancer cells after CTP-mediated cancer suppressor gene NPRL2 protein treatment. Biol Chem. 2016;397(11):1163-1171. doi:10.1515/hsz-2016-0143(IF:2.710)
[91] Lin K, Qu H, Tan Y, Deng T, Gao B, Wei N. Effects of the diphenylheptane extract of Alpinia officinarum rhizomes on ethanol-induced gastric ulcers in mice. Iran J Basic Med Sci. 2021;24(5):657-665. doi:10.22038/ijbms.2021.53644.12068(IF:2.699)
[92] Peng H, Jin L, Zhang Q, et al. Calycosin Improves Intestinal Mucosal Barrier Function after Gastrectomy in Rats through Alleviating Bacterial Translocation, Inflammation, and Oxidative Stress. Evid Based Complement Alternat Med. 2022;2022:7412331. Published 2022 Jun 26. doi:10.1155/2022/7412331(IF:2.650)
[93] Jin S, He J, Li J, Guo R, Shu Y, Liu P. MiR-873 inhibition enhances gefitinib resistance in non-small cell lung cancer cells by targeting glioma-associated oncogene homolog 1. Thorac Cancer. 2018;9(10):1262-1270. doi:10.1111/1759-7714.12830(IF:2.569)
[94] Zhai L, Chen W, Cui B, Yu B, Wang Y, Liu H. Overexpressed versican promoted cell multiplication, migration and invasion in gastric cancer. Tissue Cell. 2021;73:101611. doi:10.1016/j.tice.2021.101611(IF:2.466)
[95] Li X, Gao Y, Meng Z, Zhang C, Qi Q. Regulatory role of microRNA-30b and plasminogen activator inhibitor-1 in the pathogenesis of cognitive impairment. Exp Ther Med. 2016;11(5):1993-1998. doi:10.3892/etm.2016.3162(IF:2.447)
[96] Li M, Xia S, Shi P. DPM1 expression as a potential prognostic tumor marker in hepatocellular carcinoma. PeerJ. 2020;8:e10307. Published 2020 Nov 24. doi:10.7717/peerj.10307(IF:2.379)
[97] Yin W, Liu Y, Liu X, Ma X, Sun B, Yu Z. Metformin inhibits epithelial-mesenchymal transition of oral squamous cell carcinoma via the mTOR/HIF-1α/PKM2/STAT3 pathway. Oncol Lett. 2021;21(1):31. doi:10.3892/ol.2020.12292(IF:2.311)
[98] Zhang H, Meng F, Dong S. circSMARCA5 Promoted Osteosarcoma Cell Proliferation, Adhesion, Migration, and Invasion through a Competing Endogenous RNA Network. Biomed Res Int. 2020;2020:2539150. Published 2020 Sep 27. doi:10.1155/2020/2539150(IF:2.276)
[99] Wu M, Li X, Liu Q, Xie Y, Yuan J, Wanggou S. miR-526b-3p serves as a prognostic factor and regulates the proliferation, invasion, and migration of glioma through targeting WEE1. Cancer Manag Res. 2019;11:3099-3110. Published 2019 Apr 11. doi:10.2147/CMAR.S192361(IF:2.243)
[100] Li W, Cheng B. Knockdown of LncRNA NEAT1 inhibits myofibroblast activity in oral submucous fibrosis through miR-760/TPM1 axis. J Dent Sci. 2022;17(2):707-717. doi:10.1016/j.jds.2021.11.003(IF:2.080)
[101] Li Y, Li B, Liu Y, et al. Porphyromonas gingivalis lipopolysaccharide affects oral epithelial connections via pyroptosis. J Dent Sci. 2021;16(4):1255-1263. doi:10.1016/j.jds.2021.01.003(IF:2.080)
[102] Cheng N, Li H, Han Y, Sun S. Transcription factor Six2 induces a stem cell-like phenotype in renal cell carcinoma cells. FEBS Open Bio. 2019;9(10):1808-1816. doi:10.1002/2211-5463.12721(IF:1.959)
[103] Gao ZY, Yu LL, Shi BX, Dong ZL, Sun YJ, Ma HS. T140 Inhibits Apoptosis and Promotes Proliferation and Matrix Formation Through the SDF-1/CXC Receptor-4 Signaling Pathway in Endplate Chondrocytes of the Rat Intervertebral Discs. World Neurosurg. 2020;133:e165-e172. doi:10.1016/j.wneu.2019.08.140(IF:1.723)
[104] Pan X, Li B, Zhang G, et al. Identification of RORγ as a favorable biomarker for colon cancer. J Int Med Res. 2021;49(5):3000605211008338. doi:10.1177/03000605211008338(IF:1.671)
[105] Yang Z, Peng Y, Yang S. MicroRNA-146a regulates the transformation from liver fibrosis to cirrhosis in patients with hepatitis B via interleukin-6. Exp Ther Med. 2019;17(6):4670-4676. doi:10.3892/etm.2019.7490(IF:1.448)
[106] Cui J, Gong C, Cao B, Li L. MicroRNA-27a participates in the pathological process of depression in rats by regulating VEGFA. Exp Ther Med. 2018;15(5):4349-4355. doi:10.3892/etm.2018.5942(IF:1.410)
[107] Li B, Hu J, Chen X. MicroRNA-30b protects myocardial cell function in patients with acute myocardial ischemia by targeting plasminogen activator inhibitor-1. Exp Ther Med. 2018;15(6):5125-5132. doi:10.3892/etm.2018.6039(IF:1.410)
[108] Wu W, Li Y. Lung injury caused by paraquat poisoning results in increased interleukin-6 and decreased microRNA-146a levels. Exp Ther Med. 2018;16(1):406-412. doi:10.3892/etm.2018.6153(IF:1.410)
[109] Li X, Wu Z, He B, Zhong W. Tetrandrine alleviates symptoms of rheumatoid arthritis in rats by regulating the expression of cyclooxygenase-2 and inflammatory factors. Exp Ther Med. 2018;16(3):2670-2676. doi:10.3892/etm.2018.6498(IF:1.410)
[110] Jin J, Yao J, Yue F, Jin Z, Li D, Wang S. Decreased expression of microRNA-214 contributes to imatinib mesylate resistance of chronic myeloid leukemia patients by upregulating ABCB1 gene expression. Exp Ther Med. 2018;16(3):1693-1700. doi:10.3892/etm.2018.6404(IF:1.410)
[111] Zhang Y, Sun L, Sun H, et al. MicroRNA-381 protects myocardial cell function in children and mice with viral myocarditis via targeting cyclooxygenase-2 expression. Exp Ther Med. 2018;15(6):5510-5516. doi:10.3892/etm.2018.6082(IF:1.410)
[112] Chen Q, Zhao T, Xie X, et al. MicroRNA-663 regulates the proliferation of fibroblasts in hypertrophic scars via transforming growth factor-β1. Exp Ther Med. 2018;16(2):1311-1317. doi:10.3892/etm.2018.6350(IF:1.410)
[113] Song K, Li L, Sun G, Wei Y. MicroRNA-381 regulates the occurrence and immune responses of coronary atherosclerosis via cyclooxygenase-2. Exp Ther Med. 2018;15(5):4557-4563. doi:10.3892/etm.2018.5947(IF:1.410)
[114] Wang L, Gao H, Gong N, Gong M. Downregulation of microRNA-497 is associated with upregulation of synuclein γ in patients with osteosarcoma. Exp Ther Med. 2016;12(6):3761-3766. doi:10.3892/etm.2016.3838(IF:1.280)
[115] Song Q, Li H, Shao H, Li C, Lu X. MicroRNA-365 in macrophages regulates Mycobacterium tuberculosis-induced active pulmonary tuberculosis via interleukin-6. Int J Clin Exp Med. 2015;8(9):15458-15465. Published 2015 Sep 15. (IF:1.277)
[116] Zhang Y, Lin X, Zhang L, Hong W, Zeng K. MicroRNA-222 regulates the viability of fibroblasts in hypertrophic scars via matrix metalloproteinase 1. Exp Ther Med. 2018;15(2):1803-1808. doi:10.3892/etm.2017.5634(IF:1.261)
[117] He X, Ping J, Wen D. MicroRNA-186 regulates the invasion and metastasis of bladder cancer via vascular endothelial growth factor C. Exp Ther Med. 2017;14(4):3253-3258. doi:10.3892/etm.2017.4908(IF:1.261)
[118] Niu Q, Li X, Xia D, et al. MicroRNA-186 affects the proliferation of tumor cells via yes-associated protein 1 in the occurrence and development of pancreatic cancer. Exp Ther Med. 2017;14(3):2094-2100. doi:10.3892/etm.2017.4770(IF:1.261)
[119] Gong Y, Yang H, Tian X. Elucidating the mechanism of miRNA-214 in the regulation of gingival carcinoma. Exp Ther Med. 2017;13(5):2544-2550. doi:10.3892/etm.2017.4264(IF:1.261)
[120] Wang F, Wang J, Zhang Z, Chen S. Tetrandrine inhibits the proliferation and cytokine production induced by IL-22 in HaCaT cells. J Int Med Res. 2018;46(12):5210-5218. doi:10.1177/0300060518801463(IF:1.023)
[121] Liu ZJ, Chen SG, Yang YZ, et al. miR-29a inhibits adhesion, migration, and invasion of osteosarcoma cells by suppressing CDC42. Int J Clin Exp Pathol. 2019;12(11):4171-4180. Published 2019 Nov 1. (IF:0.205)

产品简介

TRIeasyTM Total RNA Extraction Reagent是一种适用于各种动植物、细菌组织、细胞的总RNA抽提试剂，具有极强的裂解能力，可在短时间内裂解细胞和组织样本，并有效抑制样本中RNA的降解，保持RNA的完整性。样品在该试剂中能够充分裂解，之后加入氯仿离心分层，形成上清层、中间层和有机层(鲜红色下层)，RNA分布在上层水相中，收集上清层后，经异丙醇沉淀便可得到总RNA。提取的总RNA纯度高，基本不含蛋白质及基因组DNA，可直接用于Northern、点杂交、mRNA纯化、体外翻译、RT-PCR、poly(A)+选择、RNA酶保护分析以及构建cDNA文库等多种分子生物学实验。

此外，样品中的DNA和蛋白也能以沉淀的方式还原。乙醇沉淀能析出中间层的DNA，而在有机层中加入异丙醇能沉淀出蛋白。本品操作简单快速，所有操作可在一小时内完成。对少量的组织(50-100 mg)和细胞(5×106)以及大量的组织(≥1 g)和细胞(>107)均有较好的裂解效果。

 

产品信息

货号

10606ES60

规格

100 mL

 

储存条件

2~8℃避光保存，有效期1年。

 

使用说明

表1 每毫升Total RNA Extraction Reagent能够充分裂解的最大样本量

样本类型

样本使用量

贴壁细胞

10 cm2培养面积

悬浮动植物细胞或酵母细胞

5×106-1×107个

细菌

107个

动物组织

50-100 mg

植物组织(多糖和多酚含量不高的)

50-100 mg

【注】：过多的样本量可能会导致裂解不充分，并使产物纯度降低。

 

注意事项

1. 本产品中含有苯酚，具有毒性和腐蚀性。如果吸入体内、接触皮肤、吞食等会引起中毒、灼伤及其他身体伤害。使用时应穿戴防护物，如防护服装、手套、眼罩、面罩等。如果不小心接触到眼睛时，应立即用大量的水冲洗并前往医院治疗。

2. 请穿实验服并佩戴一次性手套操作，避免RNase污染。

3. 需自备氯仿、异丙醇（新开封或提取RNA专用）、75%乙醇 (DEPC处理水配制)、DEPC和DEPC处理水。

4. 样品用Total RNA Extraction Reagent匀浆后，如果不加入氯仿进行下游实验，可先-70℃冻存，可保存一个月以上。

5. RNA沉淀在75%乙醇中，4℃可保存1周，-20℃可保存1年。

6. RNA半衰期比较短，易降解，建议抽提后尽快进行后续实验。

7. 本产品仅作科研用途！

 

Ver.CN20230809

Q：10606和19201的区别是什么？

A：19201是浓缩形式，需要的试剂用量更少一点，提取的准确性和提取反应的速度更快一些。一般10606就可以满足常规提取需求，如果客户想好更好的提取效果和rnase抑制效果可以用19201。

q: 用在贴壁细胞上总是不能完全从培养瓶（皿）脱落，是裂解不完全吗？

A: 这并不意味着裂解不完全。此时，细胞膜实际已完全裂开，并释放RNA，继续后续实验即可。

[1] Wang Y, Wang Y, Song D, et al. An RNA-cleaving threose nucleic acid enzyme capable of single point mutation discrimination. Nat Chem. 2022;14(3):350-359. doi:10.1038/s41557-021-00847-3(IF:24.427)
[2] Niu B, Liu J, Lv B, et al. Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis. Nat Commun. 2021;12(1):2809. Published 2021 May 14. doi:10.1038/s41467-021-23048-5(IF:14.919)
[3] Shui S, Zhao Z, Wang H, Conrad M, Liu G. Non-enzymatic lipid peroxidation initiated by photodynamic therapy drives a distinct ferroptosis-like cell death pathway. Redox Biol. 2021;45:102056. doi:10.1016/j.redox.2021.102056(IF:11.799)
[4] Fang Y, He Y, Wu C, et al. Magnetism-mediated targeting hyperthermia-immunotherapy in "cold" tumor with CSF1R inhibitor. Theranostics. 2021;11(14):6860-6872. Published 2021 May 3. doi:10.7150/thno.57511(IF:11.556)
[5] Jiang L, Yin X, Chen YH, et al. Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I. Theranostics. 2021;11(4):1703-1720. Published 2021 Jan 1. doi:10.7150/thno.43895(IF:11.556)
[6] Tang X, Deng Z, Ding P, et al. A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing. J Exp Clin Cancer Res. 2022;41(1):85. Published 2022 Mar 8. doi:10.1186/s13046-022-02276-7(IF:11.161)
[7] Zhou W, Li X, Wang Y, et al. Physiological and transcriptomic changes of zebrafish (Danio rerio) embryos-larvae in response to 2-MIB exposure. J Hazard Mater. 2021;416:126142. doi:10.1016/j.jhazmat.2021.126142(IF:10.588)
[8] Mo X, Du S, Chen X, et al. Lactate Induces Production of the tRNAHis Half to Promote B-lymphoblastic Cell Proliferation. Mol Ther. 2020;28(11):2442-2457. doi:10.1016/j.ymthe.2020.09.010(IF:8.986)
[9] Hao W, Han J, Chu Y, et al. Lower fluidity of supported lipid bilayers promotes neuronal differentiation of neural stem cells by enhancing focal adhesion formation. Biomaterials. 2018;161:106-116. doi:10.1016/j.biomaterials.2018.01.034(IF:8.806)
[10] Zou G, Zhang X, Wang L, et al. Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription. Theranostics. 2020;10(15):6839-6853. Published 2020 May 22. doi:10.7150/thno.43622(IF:8.579)
[11] Zhou X, Wang G, An X, et al. Polystyrene microplastic particles: In vivo and in vitro ocular surface toxicity assessment. Environ Pollut. 2022;303:119126. doi:10.1016/j.envpol.2022.119126(IF:8.071)
[12] Zhou W, Wang Y, Wang J, et al. β-Ionone causes endocrine disruption, hyperpigmentation and hypoactivity in zebrafish early life stages. Sci Total Environ. 2022;834:155433. doi:10.1016/j.scitotenv.2022.155433(IF:7.963)
[13] Zhao P, Zhang J, Wu A, et al. Biomimetic codelivery overcomes osimertinib-resistant NSCLC and brain metastasis via macrophage-mediated innate immunity. J Control Release. 2021;329:1249-1261. doi:10.1016/j.jconrel.2020.10.052(IF:7.727)
[14] Wu Z, Lu Z, Li L, et al. Identification and Validation of Ferroptosis-Related LncRNA Signatures as a Novel Prognostic Model for Colon Cancer. Front Immunol. 2022;12:783362. Published 2022 Jan 26. doi:10.3389/fimmu.2021.783362(IF:7.561)
[15] Lv Y, Wang X, Li X, et al. Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway. PLoS Biol. 2020;18(11):e3000872. Published 2020 Nov 13. doi:10.1371/journal.pbio.3000872(IF:7.076)
[16] Guo Q, Wang T, Yang Y, et al. Transcriptional Factor Yin Yang 1 Promotes the Stemness of Breast Cancer Cells by Suppressing miR-873-5p Transcriptional Activity. Mol Ther Nucleic Acids. 2020;21:527-541. doi:10.1016/j.omtn.2020.06.018(IF:7.032)
[17] Duan Y, Jiang N, Chen J, Chen J. Expression, localization and metabolic function of "resurrected" human urate oxidase in human hepatocytes. Int J Biol Macromol. 2021;175:30-39. doi:10.1016/j.ijbiomac.2021.01.163(IF:6.953)
[18] Liu X, Wang C, Pang L, Pan L, Zhang Q. Combination of resolvin E1 and lipoxin A4 promotes the resolution of pulpitis by inhibiting NF-κB activation through upregulating sirtuin 7 in dental pulp fibroblasts. Cell Prolif. 2022;55(5):e13227. doi:10.1111/cpr.13227(IF:6.831)
[19] Luo X, Sun X, Wang Y, et al. Clinical Study of 8 Cases of CHD2 Gene Mutation-Related Neurological Diseases and Their Mechanisms. Front Cell Dev Biol. 2022;10:853127. Published 2022 Mar 21. doi:10.3389/fcell.2022.853127(IF:6.684)
[20] Wang Y, Zhao M, Li W, et al. BMSC-Derived Small Extracellular Vesicles Induce Cartilage Reconstruction of Temporomandibular Joint Osteoarthritis via Autotaxin-YAP Signaling Axis. Front Cell Dev Biol. 2021;9:656153. Published 2021 Apr 1. doi:10.3389/fcell.2021.656153(IF:6.684)
[21] Han S, Cao D, Sha J, Zhu X, Chen D. LncRNA ZFPM2-AS1 promotes lung adenocarcinoma progression by interacting with UPF1 to destabilize ZFPM2. Mol Oncol. 2020;14(5):1074-1088. doi:10.1002/1878-0261.12631(IF:6.574)
[22] Wang J, Hu R, Wang Z, et al. Establishment of Immortalized Yak Ruminal Epithelial Cell Lines by Lentivirus-Mediated SV40T and hTERT Gene Transduction. Oxid Med Cell Longev. 2022;2022:8128028. Published 2022 Mar 25. doi:10.1155/2022/8128028(IF:6.543)
[23] Fan H, Wang S, Wang H, Sun M, Wu S, Bao W. Melatonin Ameliorates the Toxicity Induced by Deoxynivalenol in Murine Ovary Granulosa Cells by Antioxidative and Anti-Inflammatory Effects. Antioxidants (Basel). 2021;10(7):1045. Published 2021 Jun 29. doi:10.3390/antiox10071045(IF:6.313)
[24] Wang Y, Du S, Zhu C, et al. STUB1 is targeted by the SUMO-interacting motif of EBNA1 to maintain Epstein-Barr Virus latency. PLoS Pathog. 2020;16(3):e1008447. Published 2020 Mar 16. doi:10.1371/journal.ppat.1008447(IF:6.218)
[25] Li A, Liu Q, Li Q, Liu B, Yang Y, Zhang N. Berberine Reduces Pyruvate-driven Hepatic Glucose Production by Limiting Mitochondrial Import of Pyruvate through Mitochondrial Pyruvate Carrier 1. EBioMedicine. 2018;34:243-255. doi:10.1016/j.ebiom.2018.07.039(IF:6.183)
[26] Chen LS, Zhang M, Chen P, et al. The m6A demethylase FTO promotes the osteogenesis of mesenchymal stem cells by downregulating PPARG. Acta Pharmacol Sin. 2022;43(5):1311-1323. doi:10.1038/s41401-021-00756-8(IF:6.150)
[27] Xue H, Lu J, Yan H, et al. γ-Glutamyl transpeptidase-activated indole-quinolinium based cyanine as a fluorescence turn-on nucleolus-targeting probe for cancer cell detection and inhibition. Talanta. 2022;237:122898. doi:10.1016/j.talanta.2021.122898(IF:6.057)
[28] Yang N, Zhang X, Li L, et al. Ginsenoside Rc Promotes Bone Formation in Ovariectomy-Induced Osteoporosis In Vivo and Osteogenic Differentiation In Vitro. Int J Mol Sci. 2022;23(11):6187. Published 2022 May 31. doi:10.3390/ijms23116187(IF:5.924)
[29] Song J, Meng Y, Wang M, et al. Mangiferin activates Nrf2 to attenuate cardiac fibrosis via redistributing glutaminolysis-derived glutamate. Pharmacol Res. 2020;157:104845. doi:10.1016/j.phrs.2020.104845(IF:5.893)
[30] Zhenzhen L, Wenting L, Jianmin Z, et al. miR-146a-5p/TXNIP axis attenuates intestinal ischemia-reperfusion injury by inhibiting autophagy via the PRKAA/mTOR signaling pathway. Biochem Pharmacol. 2022;197:114839. doi:10.1016/j.bcp.2021.114839(IF:5.858)
[31] Yan H, Lu J, Wang J, et al. Prevention of Cyclophosphamide-Induced Immunosuppression in Mice With Traditional Chinese Medicine Xuanfei Baidu Decoction [published correction appears in Front Pharmacol. 2022 Jan 20;12:808424]. Front Pharmacol. 2021;12:730567. Published 2021 Oct 19. doi:10.3389/fphar.2021.730567(IF:5.811)
[32] Wei W, Cao T, Pathak JL, et al. Apigenin, a Single Active Component of Herbal Extract, Alleviates Xerostomia via ERα-Mediated Upregulation of AQP5 Activation. Front Pharmacol. 2022;13:818116. Published 2022 Feb 21. doi:10.3389/fphar.2022.818116(IF:5.811)
[33] Chen Y, Chen J, Shu A, et al. Combination of the Herbs Radix Rehmanniae and Cornus Officinalis Mitigated Testicular Damage From Diabetes Mellitus by Enhancing Glycolysis via the AGEs/RAGE/HIF-1α Axis. Front Pharmacol. 2021;12:678300. Published 2021 Jun 28. doi:10.3389/fphar.2021.678300(IF:5.811)
[34] Cao L, Lu X, Wang G, et al. Maize ZmbZIP33 Is Involved in Drought Resistance and Recovery Ability Through an Abscisic Acid-Dependent Signaling Pathway. Front Plant Sci. 2021;12:629903. Published 2021 Apr 1. doi:10.3389/fpls.2021.629903(IF:5.754)
[35] Cheng H, Fan X, Wang X, et al. Hierarchically Self-Assembled Supramolecular Host-Guest Delivery System for Drug Resistant Cancer Therapy. Biomacromolecules. 2018;19(6):1926-1938. doi:10.1021/acs.biomac.7b01693(IF:5.738)
[36] Bao L, Yuan L, Li P, et al. A FUS-LATS1/2 Axis Inhibits Hepatocellular Carcinoma Progression via Activating Hippo Pathway. Cell Physiol Biochem. 2018;50(2):437-451. doi:10.1159/000494155(IF:5.500)
[37] Wang J, Li HY, Wang HS, Su ZB. MicroRNA-485 Modulates the TGF-β/ Smads Signaling Pathway in Chronic Asthmatic Mice by Targeting Smurf2 [retracted in: Cell Physiol Biochem. 2021;55(5):658]. Cell Physiol Biochem. 2018;51(2):692-710. doi:10.1159/000495327(IF:5.500)
[38] Li J, Yang YL, Li LZ, et al. Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: Therapeutic effects of ginsenoside Rb1. Biochim Biophys Acta Mol Basis Dis. 2017;1863(11):2835-2847. doi:10.1016/j.bbadis.2017.07.017(IF:5.476)
[39] Fan X, Cheng H, Wang X, et al. Thermoresponsive Supramolecular Chemotherapy by "V"-Shaped Armed β-Cyclodextrin Star Polymer to Overcome Drug Resistance. Adv Healthc Mater. 2018;7(7):e1701143. doi:10.1002/adhm.201701143(IF:5.110)
[40] Li C, Zhu Y, Wu Y, et al. Oridonin Alleviates LPS-Induced Depression by Inhibiting NLRP3 Inflammasome via Activation of Autophagy. Front Med (Lausanne). 2022;8:813047. Published 2022 Jan 12. doi:10.3389/fmed.2021.813047(IF:5.093)
[41] He ZD, Zhang M, Wang YH, et al. Anti-PD-L1 mediating tumor-targeted codelivery of liposomal irinotecan/JQ1 for chemo-immunotherapy. Acta Pharmacol Sin. 2021;42(9):1516-1523. doi:10.1038/s41401-020-00570-8(IF:5.064)
[42] Liu Y, Chen D, Zhang X, et al. Construction of Baculovirus-Inducible CRISPR/Cas9 Antiviral System Targeting BmNPV in Bombyx mori. Viruses. 2021;14(1):59. Published 2021 Dec 30. doi:10.3390/v14010059(IF:5.048)
[43] Hu R, Zhu X, Chen C, Xu R, Li Y, Xu W. RNA-binding protein PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3'UTR with miRNAs. Cell Prolif. 2018;51(6):e12508. doi:10.1111/cpr.12508(IF:4.936)
[44] Li Z, Wang Y, Hu R, Xu R, Xu W. LncRNA B4GALT1-AS1 recruits HuR to promote osteosarcoma cells stemness and migration via enhancing YAP transcriptional activity. Cell Prolif. 2018;51(6):e12504. doi:10.1111/cpr.12504(IF:4.936)
[45] Wei R, Zhong S, Qiao L, et al. Steroid 5α-Reductase Type I Induces Cell Viability and Migration via Nuclear Factor-κB/Vascular Endothelial Growth Factor Signaling Pathway in Colorectal Cancer. Front Oncol. 2020;10:1501. Published 2020 Aug 28. doi:10.3389/fonc.2020.01501(IF:4.848)
[46] Wang S, He X, Li Q, et al. Obstructive Sleep Apnea Affects Lacrimal Gland Function. Invest Ophthalmol Vis Sci. 2022;63(3):3. doi:10.1167/iovs.63.3.3(IF:4.799)
[47] Zhou X , Weng W , Chen B , et al. Mesoporous silica nanoparticles/gelatin porous composite scaffolds with localized and sustained release of vancomycin for treatment of infected bone defects. J Mater Chem B. 2018;6(5):740-752. doi:10.1039/c7tb01246b(IF:4.776)
[48] Li Z, Liu S, Fu T, Peng Y, Zhang J. Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells. Stem Cell Res Ther. 2019;10(1):351. Published 2019 Nov 27. doi:10.1186/s13287-019-1441-4(IF:4.627)
[49] Wang J, Ren D, Sun Y, et al. Inhibition of PLK4 might enhance the anti-tumour effect of bortezomib on glioblastoma via PTEN/PI3K/AKT/mTOR signalling pathway. J Cell Mol Med. 2020;24(7):3931-3947. doi:10.1111/jcmm.14996(IF:4.486)
[50] Karuppiah V, Vallikkannu M, Li T, Chen J. Simultaneous and sequential based co-fermentations of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841: a strategy to enhance the gene expression and metabolites to improve the bio-control and plant growth promoting activity. Microb Cell Fact. 2019;18(1):185. Published 2019 Oct 29. doi:10.1186/s12934-019-1233-7(IF:4.402)
[51] Wu Y, Min L, Xu Y, et al. Combination of molecular docking and liver transcription sequencing analysis for the evaluation of salt-processed psoraleae fructus-induced hepatotoxicity in ovariectomized mice. J Ethnopharmacol. 2022;288:114955. doi:10.1016/j.jep.2021.114955(IF:4.360)
[52] Karuppiah V, Sun J, Li T, Vallikkannu M, Chen J. Co-cultivation of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841 Causes Differential Gene Expression and Improvement in the Wheat Growth and Biocontrol Activity. Front Microbiol. 2019;10:1068. Published 2019 May 16. doi:10.3389/fmicb.2019.01068(IF:4.259)
[53] Tang H, Yu Q, Li Z, et al. A PIP-mediated osmotic stress signaling cascade plays a positive role in the salt tolerance of sugarcane. BMC Plant Biol. 2021;21(1):589. Published 2021 Dec 13. doi:10.1186/s12870-021-03369-9(IF:4.215)
[54] Zhu W, Hu J, Li Y, et al. Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca2+ in the Primordium Differentiation. Int J Mol Sci. 2019;20(24):6317. Published 2019 Dec 14. doi:10.3390/ijms20246317(IF:4.183)
[55] Yin X, Wei Y, Song W, et al. Melatonin as an inducer of arecoline and their coordinated roles in anti-oxidative activity and immune responses. Food Funct. 2020;11(10):8788-8799. doi:10.1039/d0fo01841d(IF:4.171)
[56] Sun T, Zhang L, Feng J, et al. Characterization of cellular senescence in doxorubicin-induced aging mice. Exp Gerontol. 2022;163:111800. doi:10.1016/j.exger.2022.111800(IF:4.032)
[57] Wang W, Wang L, Wang L, et al. Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencing. BMC Genomics. 2021;22(1):109. Published 2021 Feb 9. doi:10.1186/s12864-021-07416-5(IF:3.969)
[58] Qiu BQ, Zhang PF, Xiong D, et al. CircRNA fibroblast growth factor receptor 3 promotes tumor progression in non-small cell lung cancer by regulating Galectin-1-AKT/ERK1/2 signaling. J Cell Physiol. 2019;234(7):11256-11264. doi:10.1002/jcp.27783(IF:3.923)
[59] Xu J, Luo X, Fang G, et al. Transgenic expression of antimicrobial peptides from black soldier fly enhance resistance against entomopathogenic bacteria in the silkworm, Bombyx mori. Insect Biochem Mol Biol. 2020;127:103487. doi:10.1016/j.ibmb.2020.103487(IF:3.827)
[60] Liao Y, Zhang H, He D, et al. Retinal Pigment Epithelium Cell Death Is Associated With NLRP3 Inflammasome Activation by All-trans Retinal. Invest Ophthalmol Vis Sci. 2019;60(8):3034-3045. doi:10.1167/iovs.18-26360(IF:3.812)
[61] Li Z, Zhang X, Yuan T, et al. Addition of Platelet-Rich Plasma to Silk Fibroin Hydrogel Bioprinting for Cartilage Regeneration. Tissue Eng Part A. 2020;26(15-16):886-895. doi:10.1089/ten.TEA.2019.0304(IF:3.776)
[62] Wang C, Wang Y, Wang C, et al. Therapeutic application of 3B-PEG injectable hydrogel/Nell-1 composite system to temporomandibular joint osteoarthritis. Biomed Mater. 2021;17(1):10.1088/1748-605X/ac367f. Published 2021 Nov 19. doi:10.1088/1748-605X/ac367f(IF:3.715)
[63] Wang M, Tao H, Huang P. Clinical significance of LARGE1 in progression of liver cancer and the underlying mechanism. Gene. 2021;779:145493. doi:10.1016/j.gene.2021.145493(IF:3.688)
[64] Tong X, Wang Y, Sun A, Bello BK, Ni S, Zhang J. Notched Belly Grain 4, a Novel Allele of Dwarf 11, Regulates Grain Shape and Seed Germination in Rice (Oryza sativa L.). Int J Mol Sci. 2018;19(12):4069. Published 2018 Dec 16. doi:10.3390/ijms19124069(IF:3.687)
[65] Tong X, Wang Y, Sun A, Bello BK, Ni S, Zhang J. Notched Belly Grain 4, a Novel Allele of Dwarf 11, Regulates Grain Shape and Seed Germination in Rice (Oryza sativa L.). Int J Mol Sci. 2018;19(12):4069. Published 2018 Dec 16. doi:10.3390/ijms19124069(IF:3.687)
[66] Zhu Y, Du Q, Jiao N, et al. Catalpol ameliorates diabetes-induced testicular injury and modulates gut microbiota. Life Sci. 2021;267:118881. doi:10.1016/j.lfs.2020.118881(IF:3.647)
[67] Chen L, Qin L, Chen C, Hu Q, Wang J, Shen J. Serum exosomes accelerate diabetic wound healing by promoting angiogenesis and ECM formation. Cell Biol Int. 2021;45(9):1976-1985. doi:10.1002/cbin.11627(IF:3.612)
[68] Cai Y, Liu Y, Sun Y, Ren Y. Mesenchyme homeobox 2 has a cancer-inhibiting function in breast carcinoma via affection of the PI3K/AKT/mTOR and ERK1/2 pathways. Biochem Biophys Res Commun. 2022;593:20-27. doi:10.1016/j.bbrc.2022.01.011(IF:3.575)
[69] Yang Y, Wang L, Che Z, et al. Novel target sites for soybean yield enhancement by photosynthesis. J Plant Physiol. 2022;268:153580. doi:10.1016/j.jplph.2021.153580(IF:3.549)
[70] Qiao S, Li B, Cai Q, et al. Involvement of ferroptosis in Porphyromonas gingivalis lipopolysaccharide-stimulated periodontitis in vitro and in vivo [published online ahead of print, 2022 Jun 28]. Oral Dis. 2022;10.1111/odi.14292. doi:10.1111/odi.14292(IF:3.511)
[71] Zhang Y, Niu J, Zhang S, et al. Comparative study on the gastrointestinal- and immune- regulation functions of Hedysari Radix Paeparata Cum Melle and Astragali Radix Praeparata cum Melle in rats with spleen-qi deficiency, based on fuzzy matter-element analysis. Pharm Biol. 2022;60(1):1237-1254. doi:10.1080/13880209.2022.2086990(IF:3.503)
[72] Song H, Xu Y, Shi L, et al. LncRNA THOR increases the stemness of gastric cancer cells via enhancing SOX9 mRNA stability. Biomed Pharmacother. 2018;108:338-346. doi:10.1016/j.biopha.2018.09.057(IF:3.457)
[73] Zhang M, Li Y, Wang H, Yu W, Lin S, Guo J. LncRNA SNHG5 affects cell proliferation, metastasis and migration of colorectal cancer through regulating miR-132-3p/CREB5. Cancer Biol Ther. 2019;20(4):524-536. doi:10.1080/15384047.2018.1537579(IF:3.373)
[74] Ding Q, Sun J, Xie W, Zhang M, Zhang C, Xu X. Stemona alkaloids suppress the positive feedback loop between M2 polarization and fibroblast differentiation by inhibiting JAK2/STAT3 pathway in fibroblasts and CXCR4/PI3K/AKT1 pathway in macrophages. Int Immunopharmacol. 2019;72:385-394. doi:10.1016/j.intimp.2019.04.030(IF:3.361)
[75] Hou J, Qian J, Li Z, et al. Bioactive Compounds from Abelmoschus manihot L. Alleviate the Progression of Multiple Myeloma in Mouse Model and Improve Bone Marrow Microenvironment. Onco Targets Ther. 2020;13:959-973. Published 2020 Jan 31. doi:10.2147/OTT.S235944(IF:3.337)
[76] Ni S, Li Z, Ying J, Zhang J, Chen H. Decreased Spikelets 4 Encoding a Novel Tetratricopeptide Repeat Domain-Containing Protein Is Involved in DNA Repair and Spikelet Number Determination in Rice. Genes (Basel). 2019;10(3):214. Published 2019 Mar 13. doi:10.3390/genes10030214(IF:3.331)
[77] Chen S, Li M, Jiang W, Zheng H, Qi LW, Jiang S. The role of Neu1 in the protective effect of dipsacoside B on acetaminophen-induced liver injury. Ann Transl Med. 2020;8(13):823. doi:10.21037/atm-19-3850(IF:3.297)
[78] Cao L, Lu X, Wang G, et al. Transcriptional regulatory networks in response to drought stress and rewatering in maize (Zea mays L.). Mol Genet Genomics. 2021;296(6):1203-1219. doi:10.1007/s00438-021-01820-y(IF:3.291)
[79] Yang Z , Wu F , He Y , et al. A novel PTP1B inhibitor extracted from Ganoderma lucidum ameliorates insulin resistance by regulating IRS1-GLUT4 cascades in the insulin signaling pathway. Food Funct. 2018;9(1):397-406. doi:10.1039/c7fo01489a(IF:3.247)
[80] Li YY, Cai Q, Li BS, et al. The Effect of Porphyromonas gingivalis Lipopolysaccharide on the Pyroptosis of Gingival Fibroblasts. Inflammation. 2021;44(3):846-858. doi:10.1007/s10753-020-01379-7(IF:3.212)
[81] Gong F, Dong D, Zhang T, Xu W. Long non-coding RNA FENDRR attenuates the stemness of non-small cell lung cancer cells via decreasing multidrug resistance gene 1 (MDR1) expression through competitively binding with RNA binding protein HuR. Eur J Pharmacol. 2019;853:345-352. doi:10.1016/j.ejphar.2019.04.022(IF:3.170)
[82] Song H, Shi L, Xu Y, et al. BRD4 promotes the stemness of gastric cancer cells via attenuating miR-216a-3p-mediated inhibition of Wnt/β-catenin signaling. Eur J Pharmacol. 2019;852:189-197. doi:10.1016/j.ejphar.2019.03.018(IF:3.170)
[83] Tan JK, Ma XF, Wang GN, Jiang CR, Gong HQ, Liu H. LncRNA MIAT knockdown alleviates oxygen-glucose deprivation‑induced cardiomyocyte injury by regulating JAK2/STAT3 pathway via miR-181a-5p. J Cardiol. 2021;78(6):586-597. doi:10.1016/j.jjcc.2021.08.018(IF:3.159)
[84] Tan Q, Lin S, Zeng Y, et al. Ginsenoside Rg3 attenuates the osimertinib resistance by reducing the stemness of non-small cell lung cancer cells. Environ Toxicol. 2020;35(6):643-651. doi:10.1002/tox.22899(IF:3.118)
[85] Yang Z, Zhang Z, Zhao J, He Y, Yang H, Zhou P. Modulation of energy metabolism and mitochondrial biogenesis by a novel proteoglycan from Ganoderma lucidum. RSC Adv. 2019;9(5):2591-2598. Published 2019 Jan 18. doi:10.1039/c8ra09482a(IF:3.049)
[86] Xu L, Sheng T, Liu X, Zhang T, Wang Z, Han H. Analyzing the hepatoprotective effect of the Swertia cincta Burkillextract against ANIT-induced cholestasis in rats by modulating the expression of transporters and metabolic enzymes. J Ethnopharmacol. 2017;209:91-99. doi:10.1016/j.jep.2017.07.031(IF:2.981)
[87] Wang S, Zhang G, Zheng W, et al. MiR-454-3p and miR-374b-5p suppress migration and invasion of bladder cancer cells through targetting ZEB2. Biosci Rep. 2018;38(6):BSR20181436. Published 2018 Dec 7. doi:10.1042/BSR20181436(IF:2.899)
[88] Yang Z, Chen C, Zhao J, et al. Hypoglycemic mechanism of a novel proteoglycan, extracted from Ganoderma lucidum, in hepatocytes. Eur J Pharmacol. 2018;820:77-85. doi:10.1016/j.ejphar.2017.12.020(IF:2.896)
[89] Liu Z, Li J, Hu X, Xu H. Helicobacter pylori-induced protein tyrosine phosphatase receptor type C as a prognostic biomarker for gastric cancer. J Gastrointest Oncol. 2021;12(3):1058-1073. doi:10.21037/jgo-21-305(IF:2.892)
[90] Zeng Y, Shi XB, Yuan ZY, et al. Biological characteristics of renal cancer cells after CTP-mediated cancer suppressor gene NPRL2 protein treatment. Biol Chem. 2016;397(11):1163-1171. doi:10.1515/hsz-2016-0143(IF:2.710)
[91] Lin K, Qu H, Tan Y, Deng T, Gao B, Wei N. Effects of the diphenylheptane extract of Alpinia officinarum rhizomes on ethanol-induced gastric ulcers in mice. Iran J Basic Med Sci. 2021;24(5):657-665. doi:10.22038/ijbms.2021.53644.12068(IF:2.699)
[92] Peng H, Jin L, Zhang Q, et al. Calycosin Improves Intestinal Mucosal Barrier Function after Gastrectomy in Rats through Alleviating Bacterial Translocation, Inflammation, and Oxidative Stress. Evid Based Complement Alternat Med. 2022;2022:7412331. Published 2022 Jun 26. doi:10.1155/2022/7412331(IF:2.650)
[93] Jin S, He J, Li J, Guo R, Shu Y, Liu P. MiR-873 inhibition enhances gefitinib resistance in non-small cell lung cancer cells by targeting glioma-associated oncogene homolog 1. Thorac Cancer. 2018;9(10):1262-1270. doi:10.1111/1759-7714.12830(IF:2.569)
[94] Zhai L, Chen W, Cui B, Yu B, Wang Y, Liu H. Overexpressed versican promoted cell multiplication, migration and invasion in gastric cancer. Tissue Cell. 2021;73:101611. doi:10.1016/j.tice.2021.101611(IF:2.466)
[95] Li X, Gao Y, Meng Z, Zhang C, Qi Q. Regulatory role of microRNA-30b and plasminogen activator inhibitor-1 in the pathogenesis of cognitive impairment. Exp Ther Med. 2016;11(5):1993-1998. doi:10.3892/etm.2016.3162(IF:2.447)
[96] Li M, Xia S, Shi P. DPM1 expression as a potential prognostic tumor marker in hepatocellular carcinoma. PeerJ. 2020;8:e10307. Published 2020 Nov 24. doi:10.7717/peerj.10307(IF:2.379)
[97] Yin W, Liu Y, Liu X, Ma X, Sun B, Yu Z. Metformin inhibits epithelial-mesenchymal transition of oral squamous cell carcinoma via the mTOR/HIF-1α/PKM2/STAT3 pathway. Oncol Lett. 2021;21(1):31. doi:10.3892/ol.2020.12292(IF:2.311)
[98] Zhang H, Meng F, Dong S. circSMARCA5 Promoted Osteosarcoma Cell Proliferation, Adhesion, Migration, and Invasion through a Competing Endogenous RNA Network. Biomed Res Int. 2020;2020:2539150. Published 2020 Sep 27. doi:10.1155/2020/2539150(IF:2.276)
[99] Wu M, Li X, Liu Q, Xie Y, Yuan J, Wanggou S. miR-526b-3p serves as a prognostic factor and regulates the proliferation, invasion, and migration of glioma through targeting WEE1. Cancer Manag Res. 2019;11:3099-3110. Published 2019 Apr 11. doi:10.2147/CMAR.S192361(IF:2.243)
[100] Li W, Cheng B. Knockdown of LncRNA NEAT1 inhibits myofibroblast activity in oral submucous fibrosis through miR-760/TPM1 axis. J Dent Sci. 2022;17(2):707-717. doi:10.1016/j.jds.2021.11.003(IF:2.080)
[101] Li Y, Li B, Liu Y, et al. Porphyromonas gingivalis lipopolysaccharide affects oral epithelial connections via pyroptosis. J Dent Sci. 2021;16(4):1255-1263. doi:10.1016/j.jds.2021.01.003(IF:2.080)
[102] Cheng N, Li H, Han Y, Sun S. Transcription factor Six2 induces a stem cell-like phenotype in renal cell carcinoma cells. FEBS Open Bio. 2019;9(10):1808-1816. doi:10.1002/2211-5463.12721(IF:1.959)
[103] Gao ZY, Yu LL, Shi BX, Dong ZL, Sun YJ, Ma HS. T140 Inhibits Apoptosis and Promotes Proliferation and Matrix Formation Through the SDF-1/CXC Receptor-4 Signaling Pathway in Endplate Chondrocytes of the Rat Intervertebral Discs. World Neurosurg. 2020;133:e165-e172. doi:10.1016/j.wneu.2019.08.140(IF:1.723)
[104] Pan X, Li B, Zhang G, et al. Identification of RORγ as a favorable biomarker for colon cancer. J Int Med Res. 2021;49(5):3000605211008338. doi:10.1177/03000605211008338(IF:1.671)
[105] Yang Z, Peng Y, Yang S. MicroRNA-146a regulates the transformation from liver fibrosis to cirrhosis in patients with hepatitis B via interleukin-6. Exp Ther Med. 2019;17(6):4670-4676. doi:10.3892/etm.2019.7490(IF:1.448)
[106] Cui J, Gong C, Cao B, Li L. MicroRNA-27a participates in the pathological process of depression in rats by regulating VEGFA. Exp Ther Med. 2018;15(5):4349-4355. doi:10.3892/etm.2018.5942(IF:1.410)
[107] Li B, Hu J, Chen X. MicroRNA-30b protects myocardial cell function in patients with acute myocardial ischemia by targeting plasminogen activator inhibitor-1. Exp Ther Med. 2018;15(6):5125-5132. doi:10.3892/etm.2018.6039(IF:1.410)
[108] Wu W, Li Y. Lung injury caused by paraquat poisoning results in increased interleukin-6 and decreased microRNA-146a levels. Exp Ther Med. 2018;16(1):406-412. doi:10.3892/etm.2018.6153(IF:1.410)
[109] Li X, Wu Z, He B, Zhong W. Tetrandrine alleviates symptoms of rheumatoid arthritis in rats by regulating the expression of cyclooxygenase-2 and inflammatory factors. Exp Ther Med. 2018;16(3):2670-2676. doi:10.3892/etm.2018.6498(IF:1.410)
[110] Jin J, Yao J, Yue F, Jin Z, Li D, Wang S. Decreased expression of microRNA-214 contributes to imatinib mesylate resistance of chronic myeloid leukemia patients by upregulating ABCB1 gene expression. Exp Ther Med. 2018;16(3):1693-1700. doi:10.3892/etm.2018.6404(IF:1.410)
[111] Zhang Y, Sun L, Sun H, et al. MicroRNA-381 protects myocardial cell function in children and mice with viral myocarditis via targeting cyclooxygenase-2 expression. Exp Ther Med. 2018;15(6):5510-5516. doi:10.3892/etm.2018.6082(IF:1.410)
[112] Chen Q, Zhao T, Xie X, et al. MicroRNA-663 regulates the proliferation of fibroblasts in hypertrophic scars via transforming growth factor-β1. Exp Ther Med. 2018;16(2):1311-1317. doi:10.3892/etm.2018.6350(IF:1.410)
[113] Song K, Li L, Sun G, Wei Y. MicroRNA-381 regulates the occurrence and immune responses of coronary atherosclerosis via cyclooxygenase-2. Exp Ther Med. 2018;15(5):4557-4563. doi:10.3892/etm.2018.5947(IF:1.410)
[114] Wang L, Gao H, Gong N, Gong M. Downregulation of microRNA-497 is associated with upregulation of synuclein γ in patients with osteosarcoma. Exp Ther Med. 2016;12(6):3761-3766. doi:10.3892/etm.2016.3838(IF:1.280)
[115] Song Q, Li H, Shao H, Li C, Lu X. MicroRNA-365 in macrophages regulates Mycobacterium tuberculosis-induced active pulmonary tuberculosis via interleukin-6. Int J Clin Exp Med. 2015;8(9):15458-15465. Published 2015 Sep 15. (IF:1.277)
[116] Zhang Y, Lin X, Zhang L, Hong W, Zeng K. MicroRNA-222 regulates the viability of fibroblasts in hypertrophic scars via matrix metalloproteinase 1. Exp Ther Med. 2018;15(2):1803-1808. doi:10.3892/etm.2017.5634(IF:1.261)
[117] He X, Ping J, Wen D. MicroRNA-186 regulates the invasion and metastasis of bladder cancer via vascular endothelial growth factor C. Exp Ther Med. 2017;14(4):3253-3258. doi:10.3892/etm.2017.4908(IF:1.261)
[118] Niu Q, Li X, Xia D, et al. MicroRNA-186 affects the proliferation of tumor cells via yes-associated protein 1 in the occurrence and development of pancreatic cancer. Exp Ther Med. 2017;14(3):2094-2100. doi:10.3892/etm.2017.4770(IF:1.261)
[119] Gong Y, Yang H, Tian X. Elucidating the mechanism of miRNA-214 in the regulation of gingival carcinoma. Exp Ther Med. 2017;13(5):2544-2550. doi:10.3892/etm.2017.4264(IF:1.261)
[120] Wang F, Wang J, Zhang Z, Chen S. Tetrandrine inhibits the proliferation and cytokine production induced by IL-22 in HaCaT cells. J Int Med Res. 2018;46(12):5210-5218. doi:10.1177/0300060518801463(IF:1.023)
[121] Liu ZJ, Chen SG, Yang YZ, et al. miR-29a inhibits adhesion, migration, and invasion of osteosarcoma cells by suppressing CDC42. Int J Clin Exp Pathol. 2019;12(11):4171-4180. Published 2019 Nov 1. (IF:0.205)