InStab™磷酸酶抑制剂混合物(100×,储存液)|Phosphatase Inhibitor Cocktail

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InStab™磷酸酶抑制剂混合物(100×,储存液)|Phosphatase Inhibitor Cocktail

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产品简介

 

细胞中蛋白的磷酸化和去磷酸化是信号转导、细胞增殖、细胞分化和细胞凋亡等许多重要的生物学活动的调控开关。因此在蛋白研究过程中，对磷酸酶作用的控制具有重要的生理意义。组织和细胞中充斥着大量的酶原，经裂解后释放到体外的酶原被充分激活从而开始消化周围的蛋白。因此，在研究蛋白磷酸化途径和信号通路的过程中保护蛋白磷酸化状态是极其关键的。

本品是多种磷酸酶抑制剂的特殊混合物，广谱抑制非特异性的酸性/碱性磷酸酶以及特异性的蛋白酪氨酸磷酸酶，丝氨酸/苏氨酸蛋白磷酸酶（PPA，PP2A）等。

本品以A、B管的形式提供，2种溶液可以靶向不同的磷酸酶类型，起到协同抑制的作用。

A管含有Cantharidin、Bromotetramisole和Microcystin–LR，以DMSO的即用溶液提供，抑制碱性磷酸酶的L-同功酶和丝氨酸/苏氨酸蛋白磷酸酶，例如蛋白磷酸酶1和2A（PP1和PP2A）。B管含有Sodium orthovanadate、Sodium molybdate、Sodium tartrate、Imidazole 和 Sodium Fluoride，以ddH2O的即用溶液提供，抑制酪氨酸蛋白磷酸酶，酸性磷酸酶和碱性磷酸酶。

本品可用于WB，Co-IP，Pull-down assay，IF，IHC，激酶研究等多种分析。

 

产品信息

 

编号

组分名称

20109JP05（2mL）

20109JP20（10x2mL）

20109JP70（100x2mL）

20109-A

InStab™ Phosphatase Inhibitor Cocktail-A液（100×）

1 mL

10×1 mL

100×1 mL

20109-B

InStab™ Phosphatase Inhibitor Cocktail-B液（100×）

1 mL

10×1 mL

100×1 mL

 

储存条件

 

-25~-15℃保存，有效期1年。2~8℃保存，有效期2个月。

 

使用说明

 

本品使用前从冰箱取出回温至室温。按照1:100（v/v）的比例向样品中分别加入A液，B液，每次加入后需充分混匀。

注意：
1）A管溶剂为DMSO，B管溶剂为ddH2O，相比之下DMSO融化的速度更慢，故完全溶解A液较B液需要较长时间，温度较低时，可将A液置于25℃水浴溶解。
2）使用前将A、B液混合容易形成沉淀，需要避免这种混合方式，建议向样品中分别加入适量A液和B液。
3）本品可耐受1%的SDS，Triton，NP-40。

 

注意事项

 

1.100×存储液建议分装冻存，避免反复冻融。工作液需现配现用。

2.本品不含蛋白酶抑制剂，可根据实验需要，向待分析样品预先加入合适的蛋白酶抑制剂。

3.A管溶剂为DMSO，请务必小心操作。

4.DTT，EDTA，EGTA等可能会影响本品中含有的正钒酸钠，请小心操作。

5. 本产品仅作科研用途。

6. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

Ver.CN20230918

 

Q:该产品放置于 4℃，有固体不溶物沉淀，请问是否正常？会不会影响效果？

A：正常的，该产品是浓度较高，低温可能会有一些盐类物质析出，可以尝试温育完全融化后再使用，一般不影响使用效果。

Q:该产品是否可与 PMSF 一起加入裂解液中？

A：可以。

Q:该产品分装以后放-20℃是冻结状态，这是正常吗？

A：正常的。

Q:该产品可适用于哪些实验？

A：磷酸酶抑制剂混合物具有广谱性，本品可用于 WB，Co-IP，Pull-down assay，IF， IHC，激酶研究等多种分析。

 

[1] Zhu R, Yan T, Feng Y, et al. Mesenchymal stem cell treatment improves outcome of COVID-19 patients via multiple immunomodulatory mechanisms. Cell Res. 2021;31(12):1244-1262. doi:10.1038/s41422-021-00573-y(IF:25.617)
[2] Liu H, Zhou Y, Qiu H, et al. Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src. Cell Death Dis. 2021;12(4):284. Published 2021 Mar 17. doi:10.1038/s41419-021-03561-7(IF:8.469)
[3] Chen Z, Xie J, Li Q, et al. Human CYP enzyme-activated clastogenicity of 2-ethylhexyl diphenyl phosphate (a flame retardant) in mammalian cells. Environ Pollut. 2021;285:117527. doi:10.1016/j.envpol.2021.117527(IF:8.071)
[4] Sun J, Guo Y, Fan Y, Wang Q, Zhang Q, Lai D. Decreased expression of IDH1 by chronic unpredictable stress suppresses proliferation and accelerates senescence of granulosa cells through ROS activated MAPK signaling pathways. Free Radic Biol Med. 2021;169:122-136. doi:10.1016/j.freeradbiomed.2021.04.016(IF:7.376)
[5] Yang BY, Deng GY, Zhao RZ, et al. Porous Se@SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species-NF-κB pathway inhibition. Acta Biomater. 2019;88:392-405. doi:10.1016/j.actbio.2019.02.006(IF:6.638)
[6] Zhang Y, Li G, Zhang X, Lin L. ROS-scavenging glyco-nanoplatform for synergistic antibacterial and wound-healing therapy of bacterial keratitis. J Mater Chem B. 2022;10(24):4575-4587. Published 2022 Jun 22. doi:10.1039/d2tb00667g(IF:6.331)
[7] Deng L, Li H, Su X, et al. Chlorzoxazone, a small molecule drug, augments immunosuppressive capacity of mesenchymal stem cells via modulation of FOXO3 phosphorylation. Cell Death Dis. 2020;11(3):158. Published 2020 Mar 2. doi:10.1038/s41419-020-2357-8(IF:6.304)
[8] Zhang X, Cai Z, Wu M, Huangfu X, Li J, Liu X. Adipose Stem Cell-Derived Exosomes Recover Impaired Matrix Metabolism of Torn Human Rotator Cuff Tendons by Maintaining Tissue Homeostasis. Am J Sports Med. 2021;49(4):899-908. doi:10.1177/0363546521992469(IF:6.203)
[9] Gao C, Wang SW, Lu JC, et al. KSR2-14-3-3ζ complex serves as a biomarker and potential therapeutic target in sorafenib-resistant hepatocellular carcinoma. Biomark Res. 2022;10(1):25. Published 2022 Apr 25. doi:10.1186/s40364-022-00361-9(IF:6.148)
[10] Chen L, Xu W, Yang Q, et al. Imperatorin alleviates cancer cachexia and prevents muscle wasting via directly inhibiting STAT3. Pharmacol Res. 2020;158:104871. doi:10.1016/j.phrs.2020.104871(IF:5.893)
[11] Yang BY, Zhou ZY, Liu SY, et al. Porous Se@SiO2 Nanoparticles Enhance Wound Healing by ROS-PI3K/Akt Pathway in Dermal Fibroblasts and Reduce Scar Formation. Front Bioeng Biotechnol. 2022;10:852482. Published 2022 Mar 21. doi:10.3389/fbioe.2022.852482(IF:5.890)
[12] Huang S, Tao R, Zhou J, Qian L, Wu J. Trans-10-Hydroxy-2-Decenoic Acid Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice via Regulating the Inflammasome-Mediated Pyroptotic Pathway and Enhancing Colonic Barrier Function. Mol Nutr Food Res. 2022;66(12):e2100821. doi:10.1002/mnfr.202100821(IF:5.820)
[13] Zhou X, Jian W, Luo Q, et al. Circular RNA_0006014 promotes breast cancer progression through sponging miR-885-3p to regulate NTRK2 and PIK3/AKT pathway. Aging (Albany NY). 2022;14(7):3105-3128. doi:10.18632/aging.203996(IF:5.682)
[14] Liu B, Yang MQ, Yu TY, et al. Mast Cell Tryptase Promotes Inflammatory Bowel Disease-Induced Intestinal Fibrosis. Inflamm Bowel Dis. 2021;27(2):242-255. doi:10.1093/ibd/izaa125(IF:5.325)
[15] Jiang M, Xie H, Zhang C, et al. Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy. J Cell Mol Med. 2022;26(4):1229-1244. doi:10.1111/jcmm.17179(IF:5.310)
[16] Fang DD, Tang Q, Kong Y, et al. MDM2 inhibitor APG-115 exerts potent antitumor activity and synergizes with standard-of-care agents in preclinical acute myeloid leukemia models. Cell Death Discov. 2021;7(1):90. Published 2021 May 3. doi:10.1038/s41420-021-00465-5(IF:5.241)
[17] He L, Gao K, Liu H, Wang J, Li X, He C. Smooth muscle cell-specific knockout of interferon gamma (IFN-γ) receptor attenuates intimal hyperplasia via STAT1-KLF4 activation [published online ahead of print, 2021 May 25]. Life Sci. 2021;119651. doi:10.1016/j.lfs.2021.119651(IF:5.037)
[18] Zheng Y, Luo A, Liu X. The Imbalance of Mitochondrial Fusion/Fission Drives High-Glucose-Induced Vascular Injury. Biomolecules. 2021;11(12):1779. Published 2021 Nov 27. doi:10.3390/biom11121779(IF:4.879)
[19] Tao R, Huang S, Zhou J, et al. Neonatal Supplementation of Oleamide During Suckling Promotes Learning Ability and Memory in Adolescent Mice. J Nutr. 2022;152(3):889-898. doi:10.1093/jn/nxab442(IF:4.798)
[20] Zhang Q, Ye L, Xin F, et al. Milk Fat Globule Membrane Supplementation During Suckling Ameliorates Maternal High Fat Diet-Induced Hepatic Steatosis in Adult Male Offspring of Mice. J Nutr. 2021;151(6):1487-1496. doi:10.1093/jn/nxab026(IF:4.798)
[21] Yang C, Wang W, Zhu K, et al. Lithium chloride with immunomodulatory function for regulating titanium nanoparticle-stimulated inflammatory response and accelerating osteogenesis through suppression of MAPK signaling pathway. Int J Nanomedicine. 2019;14:7475-7488. Published 2019 Sep 12. doi:10.2147/IJN.S210834(IF:4.471)
[22] Wu Y, Min L, Xu Y, et al. Combination of molecular docking and liver transcription sequencing analysis for the evaluation of salt-processed psoraleae fructus-induced hepatotoxicity in ovariectomized mice. J Ethnopharmacol. 2022;288:114955. doi:10.1016/j.jep.2021.114955(IF:4.360)
[23] Huang S, Fu Y, Xu B, et al. Wogonoside alleviates colitis by improving intestinal epithelial barrier function via the MLCK/pMLC2 pathway. Phytomedicine. 2020;68:153179. doi:10.1016/j.phymed.2020.153179(IF:4.268)
[24] Xue S, Zou J, Liu Y, Wang M, Zhang C, Le J. Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism. Int J Mol Sci. 2019;20(9):2339. Published 2019 May 11. doi:10.3390/ijms20092339(IF:4.183)
[25] Chen CH, Di YQ, Shen QY, Wang JX, Zhao XF. The steroid hormone 20-hydroxyecdysone induces phosphorylation and aggregation of stromal interacting molecule 1 for store-operated calcium entry. J Biol Chem. 2019;294(41):14922-14936. doi:10.1074/jbc.RA119.008484(IF:4.106)
[26] Liao L, Ge M, Zhan Q, et al. PSPH Mediates the Metastasis and Proliferation of Non-small Cell Lung Cancer through MAPK Signaling Pathways. Int J Biol Sci. 2019;15(1):183-194. Published 2019 Jan 1. doi:10.7150/ijbs.29203(IF:4.067)
[27] Yang C, Li J, Zhu K, et al. Puerarin Exerts Protective Effects on Wear Particle-Induced Inflammatory Osteolysis. Front Pharmacol. 2019;10:1113. Published 2019 Oct 1. doi:10.3389/fphar.2019.01113(IF:3.845)
[28] Pan K, Huang X, Jia X. SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu. Oncol Lett. 2021;21(6):485. doi:10.3892/ol.2021.12746(IF:2.967)
[29] Ma B, Wang X, Zhang R, et al. Cigarette smoke extract stimulates PCSK9 production in HepG2 cells via ROS/NF‑κB signaling. Mol Med Rep. 2021;23(5):331. doi:10.3892/mmr.2021.11970(IF:2.952)
[30] Liao L, Yu H, Ge M, et al. Upregulation of phosphoserine phosphatase contributes to tumor progression and predicts poor prognosis in non-small cell lung cancer patients. Thorac Cancer. 2019;10(5):1203-1212. doi:10.1111/1759-7714.13064(IF:2.524)
[31] Peng H, Luo Y, Ying Y. lncRNA XIST attenuates hypoxia-induced H9c2 cardiomyocyte injury by targeting the miR-122-5p/FOXP2 axis. Mol Cell Probes. 2020;50:101500. doi:10.1016/j.mcp.2019.101500(IF:2.511)
[32] Fang J, Zheng W, Hu P, Wu J. Investigating the effect of lncRNA HOTAIR on apoptosis induced by myocardial ischemia-reperfusion injury. Mol Med Rep. 2021;23(3):169. doi:10.3892/mmr.2020.11808(IF:2.100)

产品简介

 

细胞中蛋白的磷酸化和去磷酸化是信号转导、细胞增殖、细胞分化和细胞凋亡等许多重要的生物学活动的调控开关。因此在蛋白研究过程中，对磷酸酶作用的控制具有重要的生理意义。组织和细胞中充斥着大量的酶原，经裂解后释放到体外的酶原被充分激活从而开始消化周围的蛋白。因此，在研究蛋白磷酸化途径和信号通路的过程中保护蛋白磷酸化状态是极其关键的。

本品是多种磷酸酶抑制剂的特殊混合物，广谱抑制非特异性的酸性/碱性磷酸酶以及特异性的蛋白酪氨酸磷酸酶，丝氨酸/苏氨酸蛋白磷酸酶（PPA，PP2A）等。

本品以A、B管的形式提供，2种溶液可以靶向不同的磷酸酶类型，起到协同抑制的作用。

A管含有Cantharidin、Bromotetramisole和Microcystin–LR，以DMSO的即用溶液提供，抑制碱性磷酸酶的L-同功酶和丝氨酸/苏氨酸蛋白磷酸酶，例如蛋白磷酸酶1和2A（PP1和PP2A）。B管含有Sodium orthovanadate、Sodium molybdate、Sodium tartrate、Imidazole 和 Sodium Fluoride，以ddH2O的即用溶液提供，抑制酪氨酸蛋白磷酸酶，酸性磷酸酶和碱性磷酸酶。

本品可用于WB，Co-IP，Pull-down assay，IF，IHC，激酶研究等多种分析。

 

产品信息

 

编号

组分名称

20109JP05（2mL）

20109JP20（10x2mL）

20109JP70（100x2mL）

20109-A

InStab™ Phosphatase Inhibitor Cocktail-A液（100×）

1 mL

10×1 mL

100×1 mL

20109-B

InStab™ Phosphatase Inhibitor Cocktail-B液（100×）

1 mL

10×1 mL

100×1 mL

 

储存条件

 

-25~-15℃保存，有效期1年。2~8℃保存，有效期2个月。

 

使用说明

 

本品使用前从冰箱取出回温至室温。按照1:100（v/v）的比例向样品中分别加入A液，B液，每次加入后需充分混匀。

注意：
1）A管溶剂为DMSO，B管溶剂为ddH2O，相比之下DMSO融化的速度更慢，故完全溶解A液较B液需要较长时间，温度较低时，可将A液置于25℃水浴溶解。
2）使用前将A、B液混合容易形成沉淀，需要避免这种混合方式，建议向样品中分别加入适量A液和B液。
3）本品可耐受1%的SDS，Triton，NP-40。

 

注意事项

 

1.100×存储液建议分装冻存，避免反复冻融。工作液需现配现用。

2.本品不含蛋白酶抑制剂，可根据实验需要，向待分析样品预先加入合适的蛋白酶抑制剂。

3.A管溶剂为DMSO，请务必小心操作。

4.DTT，EDTA，EGTA等可能会影响本品中含有的正钒酸钠，请小心操作。

5. 本产品仅作科研用途。

6. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

Ver.CN20230918

 

Q:该产品放置于 4℃，有固体不溶物沉淀，请问是否正常？会不会影响效果？

A：正常的，该产品是浓度较高，低温可能会有一些盐类物质析出，可以尝试温育完全融化后再使用，一般不影响使用效果。

Q:该产品是否可与 PMSF 一起加入裂解液中？

A：可以。

Q:该产品分装以后放-20℃是冻结状态，这是正常吗？

A：正常的。

Q:该产品可适用于哪些实验？

A：磷酸酶抑制剂混合物具有广谱性，本品可用于 WB，Co-IP，Pull-down assay，IF， IHC，激酶研究等多种分析。

 

[1] Zhu R, Yan T, Feng Y, et al. Mesenchymal stem cell treatment improves outcome of COVID-19 patients via multiple immunomodulatory mechanisms. Cell Res. 2021;31(12):1244-1262. doi:10.1038/s41422-021-00573-y(IF:25.617)
[2] Liu H, Zhou Y, Qiu H, et al. Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src. Cell Death Dis. 2021;12(4):284. Published 2021 Mar 17. doi:10.1038/s41419-021-03561-7(IF:8.469)
[3] Chen Z, Xie J, Li Q, et al. Human CYP enzyme-activated clastogenicity of 2-ethylhexyl diphenyl phosphate (a flame retardant) in mammalian cells. Environ Pollut. 2021;285:117527. doi:10.1016/j.envpol.2021.117527(IF:8.071)
[4] Sun J, Guo Y, Fan Y, Wang Q, Zhang Q, Lai D. Decreased expression of IDH1 by chronic unpredictable stress suppresses proliferation and accelerates senescence of granulosa cells through ROS activated MAPK signaling pathways. Free Radic Biol Med. 2021;169:122-136. doi:10.1016/j.freeradbiomed.2021.04.016(IF:7.376)
[5] Yang BY, Deng GY, Zhao RZ, et al. Porous Se@SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species-NF-κB pathway inhibition. Acta Biomater. 2019;88:392-405. doi:10.1016/j.actbio.2019.02.006(IF:6.638)
[6] Zhang Y, Li G, Zhang X, Lin L. ROS-scavenging glyco-nanoplatform for synergistic antibacterial and wound-healing therapy of bacterial keratitis. J Mater Chem B. 2022;10(24):4575-4587. Published 2022 Jun 22. doi:10.1039/d2tb00667g(IF:6.331)
[7] Deng L, Li H, Su X, et al. Chlorzoxazone, a small molecule drug, augments immunosuppressive capacity of mesenchymal stem cells via modulation of FOXO3 phosphorylation. Cell Death Dis. 2020;11(3):158. Published 2020 Mar 2. doi:10.1038/s41419-020-2357-8(IF:6.304)
[8] Zhang X, Cai Z, Wu M, Huangfu X, Li J, Liu X. Adipose Stem Cell-Derived Exosomes Recover Impaired Matrix Metabolism of Torn Human Rotator Cuff Tendons by Maintaining Tissue Homeostasis. Am J Sports Med. 2021;49(4):899-908. doi:10.1177/0363546521992469(IF:6.203)
[9] Gao C, Wang SW, Lu JC, et al. KSR2-14-3-3ζ complex serves as a biomarker and potential therapeutic target in sorafenib-resistant hepatocellular carcinoma. Biomark Res. 2022;10(1):25. Published 2022 Apr 25. doi:10.1186/s40364-022-00361-9(IF:6.148)
[10] Chen L, Xu W, Yang Q, et al. Imperatorin alleviates cancer cachexia and prevents muscle wasting via directly inhibiting STAT3. Pharmacol Res. 2020;158:104871. doi:10.1016/j.phrs.2020.104871(IF:5.893)
[11] Yang BY, Zhou ZY, Liu SY, et al. Porous Se@SiO2 Nanoparticles Enhance Wound Healing by ROS-PI3K/Akt Pathway in Dermal Fibroblasts and Reduce Scar Formation. Front Bioeng Biotechnol. 2022;10:852482. Published 2022 Mar 21. doi:10.3389/fbioe.2022.852482(IF:5.890)
[12] Huang S, Tao R, Zhou J, Qian L, Wu J. Trans-10-Hydroxy-2-Decenoic Acid Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice via Regulating the Inflammasome-Mediated Pyroptotic Pathway and Enhancing Colonic Barrier Function. Mol Nutr Food Res. 2022;66(12):e2100821. doi:10.1002/mnfr.202100821(IF:5.820)
[13] Zhou X, Jian W, Luo Q, et al. Circular RNA_0006014 promotes breast cancer progression through sponging miR-885-3p to regulate NTRK2 and PIK3/AKT pathway. Aging (Albany NY). 2022;14(7):3105-3128. doi:10.18632/aging.203996(IF:5.682)
[14] Liu B, Yang MQ, Yu TY, et al. Mast Cell Tryptase Promotes Inflammatory Bowel Disease-Induced Intestinal Fibrosis. Inflamm Bowel Dis. 2021;27(2):242-255. doi:10.1093/ibd/izaa125(IF:5.325)
[15] Jiang M, Xie H, Zhang C, et al. Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy. J Cell Mol Med. 2022;26(4):1229-1244. doi:10.1111/jcmm.17179(IF:5.310)
[16] Fang DD, Tang Q, Kong Y, et al. MDM2 inhibitor APG-115 exerts potent antitumor activity and synergizes with standard-of-care agents in preclinical acute myeloid leukemia models. Cell Death Discov. 2021;7(1):90. Published 2021 May 3. doi:10.1038/s41420-021-00465-5(IF:5.241)
[17] He L, Gao K, Liu H, Wang J, Li X, He C. Smooth muscle cell-specific knockout of interferon gamma (IFN-γ) receptor attenuates intimal hyperplasia via STAT1-KLF4 activation [published online ahead of print, 2021 May 25]. Life Sci. 2021;119651. doi:10.1016/j.lfs.2021.119651(IF:5.037)
[18] Zheng Y, Luo A, Liu X. The Imbalance of Mitochondrial Fusion/Fission Drives High-Glucose-Induced Vascular Injury. Biomolecules. 2021;11(12):1779. Published 2021 Nov 27. doi:10.3390/biom11121779(IF:4.879)
[19] Tao R, Huang S, Zhou J, et al. Neonatal Supplementation of Oleamide During Suckling Promotes Learning Ability and Memory in Adolescent Mice. J Nutr. 2022;152(3):889-898. doi:10.1093/jn/nxab442(IF:4.798)
[20] Zhang Q, Ye L, Xin F, et al. Milk Fat Globule Membrane Supplementation During Suckling Ameliorates Maternal High Fat Diet-Induced Hepatic Steatosis in Adult Male Offspring of Mice. J Nutr. 2021;151(6):1487-1496. doi:10.1093/jn/nxab026(IF:4.798)
[21] Yang C, Wang W, Zhu K, et al. Lithium chloride with immunomodulatory function for regulating titanium nanoparticle-stimulated inflammatory response and accelerating osteogenesis through suppression of MAPK signaling pathway. Int J Nanomedicine. 2019;14:7475-7488. Published 2019 Sep 12. doi:10.2147/IJN.S210834(IF:4.471)
[22] Wu Y, Min L, Xu Y, et al. Combination of molecular docking and liver transcription sequencing analysis for the evaluation of salt-processed psoraleae fructus-induced hepatotoxicity in ovariectomized mice. J Ethnopharmacol. 2022;288:114955. doi:10.1016/j.jep.2021.114955(IF:4.360)
[23] Huang S, Fu Y, Xu B, et al. Wogonoside alleviates colitis by improving intestinal epithelial barrier function via the MLCK/pMLC2 pathway. Phytomedicine. 2020;68:153179. doi:10.1016/j.phymed.2020.153179(IF:4.268)
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