β-Actin Mouse Monoclonal Antibody
一键复制产品信息 产品编号: AF0003
产品包装:100μl
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100μl
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β-Actin Mouse mAb(β-Actin小鼠单抗)(AF0003)
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价格: ¥ 446.00
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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF0003 | β-Actin Mouse Monoclonal Antibody | 100μl | 446.00元 |
| 来源 | 用途 | 交叉反应性 | 分子量 |
| Mouse | WB, ICC, IHC, IF | H, M, R | 43KDa |
WB,Western blot;IP,Immunoprecipitation;IF,Immunofluorescence;IHC,Immunohistochemistry;ICC,Immunocytochemistry;FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
| 1:1000 | – | 1:20 | 1:20 | 1:20 | – | – | – |
抗体详细信息如下:
|
About this Antibody |
|
|
Name |
β-Actin Mouse Monoclonal Antibody |
|
Category |
Monoclonal antibody (mAb); Primary antibody |
|
Isotype |
IgG1 |
|
Purification |
Affinity chromatography |
|
About the Immunogen |
|
|
Immunogen |
synthetic peptide (KLH-coupled) |
|
Gene ID |
60/11461/81822 |
|
SwissProt |
P60709/P60710/P60711 |
|
Synonyms |
BRWS1; PS1TP5BP1 |
|
Category |
Cytoskeletal Signaling |
|
Background |
Beta actin also know as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β-Actin is generally used as an internal control for experimental error. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF0003 | β-Actin Mouse Monoclonal Antibody | 100μl |
| AZ100 | Western一抗稀释液 | 100ml |
| — | 说明书 | 1份 |
保存条件：
β-Actin Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋
白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体 操作可以参考如下网页： http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片:
Fig. 1. Western blot analysis on Hela (A), NIH/3T3 (B) and PC12 (C) cell lysatesusing β-actin mousemonoclonal antibody (AF003).
Fig. 2. Immunofluorescent staining of NIH/3T3 cells using β-actin mouse monoclonalantibody (AF0003). Nucleus (blue) were stained with DAPI.
Fig. 3. Immunohistochemical analysis of paraffin-embedded mouse testis tissueusing β-actin mouse monoclonal antibody (AF0003).
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