DMEM/F-12减血清培养基 DMEM/F-12 Reduced Serum Medium
产品说明书
FAQ
COA
已发表文献
DMEM/F-12减血清培养基是在经典DMEM/F-12的基础上额外添加了乙醇胺、谷胱甘肽、抗坏血酸、胰岛素、转铁蛋白、牛血清白蛋白以及微量元素，可用于在低血清状态下维持细胞的生长，应用于减血清哺乳动物细胞培养。使用前需添加1~5%的胎牛血清及4mM的L-谷氨酰胺或丙氨酰–谷氨酰胺。由于不同种类的细胞对血清的依赖程度不同，建议针对不同细胞进行优化后以确定最终的血清浓度。与经典DMEM/F-12相比，在不影响细胞生长速率和形态的情况下，使用该配方可减少50~90%的血清用量，从而降低培养条件波动，延长同一批次血清使用的时间。
本品是用注射用水制备且含有（+）酚红，不含（-）L-谷氨酰胺和HEPES
产品参数
|
无菌检测 |
本品经无菌过滤处理 |
|
内毒素残留 |
≤1 EU/mL |
|
渗透压 |
290~330 mOsm/kg·H20 |
|
pH |
7.0~7.4 |
运输与保存方法
蓝冰运输。2~8℃避光保存，有效期一年。
产品应用
可用于MRC-5、SP2、Vero、WI-38和Jurkat等细胞培养
组分配方
|
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
Glycine |
75 |
18.75 |
0.25 |
|
L-Alanine |
89 |
4.45 |
0.05 |
|
L-Arginine hydrochloride |
211 |
147.5 |
0.6991 |
|
L-Asparagine-H2O |
150 |
7.5 |
0.05 |
|
L-Aspartic acid |
133 |
6.65 |
0.05 |
|
L-Cysteine hydrochloride-H2O |
176 |
17.56 |
0.0998 |
|
L-Cystine 2HCl |
313 |
31.29 |
0.1 |
|
L-Glutamic Acid |
147 |
7.35 |
0.05 |
|
L-Glutamine |
146 |
0 |
0 |
|
L-Histidine hydrochloride-H2O |
210 |
31.48 |
0.1499 |
|
L-Isoleucine |
131 |
54.47 |
0.4158 |
|
L-Leucine |
131 |
59.05 |
0.4508 |
|
L-Lysine hydrochloride |
183 |
91.25 |
0.4986 |
|
L-Methionine |
149 |
17.24 |
0.1157 |
|
L-Phenylalanine |
165 |
35.48 |
0.215 |
|
L-Proline |
115 |
17.25 |
0.15 |
|
L-Serine |
105 |
26.25 |
0.25 |
|
L-Threonine |
119 |
53.45 |
0.4492 |
|
L-Tryptophan |
204 |
9.02 |
0.0442 |
|
L-Tyrosine disodium salt dihydrate |
261 |
55.79 |
0.2138 |
|
L-Valine |
117 |
52.85 |
0.4517 |
|
Inorganic Salts |
|||
|
Calcium Chloride (CaCl2) (anhyd.) |
111 |
116.6 |
1.0505 |
|
Cupric sulfate (CuSO4-5H2O) |
250 |
0.0013 |
5E-06 |
|
Ferric Nitrate (Fe(NO3)3"9H2O) |
404 |
0.05 |
0.0001 |
|
Ferric sulfate (FeSO4-7H2O) |
278 |
0.417 |
0.0015 |
|
Magnesium Chloride (anhydrous) |
95 |
28.64 |
0.3015 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
120 |
48.84 |
0.407 |
|
Potassium Chloride (KCl) |
75 |
311.8 |
4.1573 |
|
Sodium Bicarbonate (NaHCO3) |
84 |
2438 |
29.024 |
|
Sodium Chloride (NaCl) |
58 |
6995.5 |
120.61 |
|
Sodium Phosphate dibasic (Na2HPO4) anhydrous |
142 |
71.02 |
0.5001 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138 |
62.5 |
0.4529 |
|
Zinc sulfate (ZnSO4-7H2O) |
288 |
0.864 |
0.003 |
|
Other Components |
|||
|
D-Glucose (Dextrose) |
180 |
3151 |
17.506 |
|
Ethanolamine |
97.54 |
1.9 |
0.0195 |
|
HEPES |
238 |
0 |
0 |
|
Hypoxanthine Na |
159 |
2.39 |
0.015 |
|
Linoleic Acid |
280 |
0.042 |
0.0001 |
|
Lipoic Acid |
206 |
0.105 |
0.0005 |
|
Phenol Red |
376.4 |
8.1 |
0.0215 |
|
Putrescine 2HCl |
161 |
0.081 |
0.0005 |
|
Sodium Pyruvate |
110 |
110 |
1 |
|
Thymidine |
242 |
0.365 |
0.0015 |
|
Proteins |
|||
|
Bovine Serum Albumin |
400 |
0 |
|
|
Human Transferrin (Holo) |
7.5 |
0 |
|
|
Insulin Recombinant |
10 |
0 |
|
|
Reducing Agents |
|||
|
Glutathione, monosodium |
307 |
1 |
0.0033 |
|
Trace Elements |
|||
|
Ammonium Metavanadate |
116.98 |
0.0003 |
3E-06 |
|
Manganous Chloride |
198 |
5E-05 |
3E-07 |
|
Sodium Selenite |
173 |
0.005 |
3E-05 |
|
Vitamins |
|||
|
Ascorbic Acid phosphate |
289.54 |
2.5 |
0.0086 |
|
Biotin |
244 |
0.0035 |
1E-05 |
|
Choline chloride |
140 |
8.98 |
0.0641 |
|
D-Calcium pantothenate |
477 |
2.24 |
0.0047 |
|
Folic Acid |
441 |
2.65 |
0.006 |
|
i-Inositol |
180 |
12.6 |
0.07 |
|
Niacinamide |
122 |
2.02 |
0.0166 |
|
Pyridoxine hydrochloride |
206 |
2 |
0.0097 |
|
Riboflavin |
376 |
0.219 |
0.0006 |
|
Thiamine hydrochloride |
337 |
2.17 |
0.0064 |
|
Vitamin B12 |
1355 |
0.68 |
0.0005 |
注意事项
1. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
2. 本产品仅供科研使用，禁止用于人体。
HB220324
DMEM/F-12减血清培养基是在经典DMEM/F-12的基础上额外添加了乙醇胺、谷胱甘肽、抗坏血酸、胰岛素、转铁蛋白、牛血清白蛋白以及微量元素，可用于在低血清状态下维持细胞的生长，应用于减血清哺乳动物细胞培养。使用前需添加1~5%的胎牛血清及4mM的L-谷氨酰胺或丙氨酰–谷氨酰胺。由于不同种类的细胞对血清的依赖程度不同，建议针对不同细胞进行优化后以确定最终的血清浓度。与经典DMEM/F-12相比，在不影响细胞生长速率和形态的情况下，使用该配方可减少50~90%的血清用量，从而降低培养条件波动，延长同一批次血清使用的时间。
本品是用注射用水制备且含有（+）酚红，不含（-）L-谷氨酰胺和HEPES
产品参数
|
无菌检测 |
本品经无菌过滤处理 |
|
内毒素残留 |
≤1 EU/mL |
|
渗透压 |
290~330 mOsm/kg·H20 |
|
pH |
7.0~7.4 |
运输与保存方法
蓝冰运输。2~8℃避光保存，有效期一年。
产品应用
可用于MRC-5、SP2、Vero、WI-38和Jurkat等细胞培养
组分配方
|
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
Glycine |
75 |
18.75 |
0.25 |
|
L-Alanine |
89 |
4.45 |
0.05 |
|
L-Arginine hydrochloride |
211 |
147.5 |
0.6991 |
|
L-Asparagine-H2O |
150 |
7.5 |
0.05 |
|
L-Aspartic acid |
133 |
6.65 |
0.05 |
|
L-Cysteine hydrochloride-H2O |
176 |
17.56 |
0.0998 |
|
L-Cystine 2HCl |
313 |
31.29 |
0.1 |
|
L-Glutamic Acid |
147 |
7.35 |
0.05 |
|
L-Glutamine |
146 |
0 |
0 |
|
L-Histidine hydrochloride-H2O |
210 |
31.48 |
0.1499 |
|
L-Isoleucine |
131 |
54.47 |
0.4158 |
|
L-Leucine |
131 |
59.05 |
0.4508 |
|
L-Lysine hydrochloride |
183 |
91.25 |
0.4986 |
|
L-Methionine |
149 |
17.24 |
0.1157 |
|
L-Phenylalanine |
165 |
35.48 |
0.215 |
|
L-Proline |
115 |
17.25 |
0.15 |
|
L-Serine |
105 |
26.25 |
0.25 |
|
L-Threonine |
119 |
53.45 |
0.4492 |
|
L-Tryptophan |
204 |
9.02 |
0.0442 |
|
L-Tyrosine disodium salt dihydrate |
261 |
55.79 |
0.2138 |
|
L-Valine |
117 |
52.85 |
0.4517 |
|
Inorganic Salts |
|||
|
Calcium Chloride (CaCl2) (anhyd.) |
111 |
116.6 |
1.0505 |
|
Cupric sulfate (CuSO4-5H2O) |
250 |
0.0013 |
5E-06 |
|
Ferric Nitrate (Fe(NO3)3"9H2O) |
404 |
0.05 |
0.0001 |
|
Ferric sulfate (FeSO4-7H2O) |
278 |
0.417 |
0.0015 |
|
Magnesium Chloride (anhydrous) |
95 |
28.64 |
0.3015 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
120 |
48.84 |
0.407 |
|
Potassium Chloride (KCl) |
75 |
311.8 |
4.1573 |
|
Sodium Bicarbonate (NaHCO3) |
84 |
2438 |
29.024 |
|
Sodium Chloride (NaCl) |
58 |
6995.5 |
120.61 |
|
Sodium Phosphate dibasic (Na2HPO4) anhydrous |
142 |
71.02 |
0.5001 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138 |
62.5 |
0.4529 |
|
Zinc sulfate (ZnSO4-7H2O) |
288 |
0.864 |
0.003 |
|
Other Components |
|||
|
D-Glucose (Dextrose) |
180 |
3151 |
17.506 |
|
Ethanolamine |
97.54 |
1.9 |
0.0195 |
|
HEPES |
238 |
0 |
0 |
|
Hypoxanthine Na |
159 |
2.39 |
0.015 |
|
Linoleic Acid |
280 |
0.042 |
0.0001 |
|
Lipoic Acid |
206 |
0.105 |
0.0005 |
|
Phenol Red |
376.4 |
8.1 |
0.0215 |
|
Putrescine 2HCl |
161 |
0.081 |
0.0005 |
|
Sodium Pyruvate |
110 |
110 |
1 |
|
Thymidine |
242 |
0.365 |
0.0015 |
|
Proteins |
|||
|
Bovine Serum Albumin |
400 |
0 |
|
|
Human Transferrin (Holo) |
7.5 |
0 |
|
|
Insulin Recombinant |
10 |
0 |
|
|
Reducing Agents |
|||
|
Glutathione, monosodium |
307 |
1 |
0.0033 |
|
Trace Elements |
|||
|
Ammonium Metavanadate |
116.98 |
0.0003 |
3E-06 |
|
Manganous Chloride |
198 |
5E-05 |
3E-07 |
|
Sodium Selenite |
173 |
0.005 |
3E-05 |
|
Vitamins |
|||
|
Ascorbic Acid phosphate |
289.54 |
2.5 |
0.0086 |
|
Biotin |
244 |
0.0035 |
1E-05 |
|
Choline chloride |
140 |
8.98 |
0.0641 |
|
D-Calcium pantothenate |
477 |
2.24 |
0.0047 |
|
Folic Acid |
441 |
2.65 |
0.006 |
|
i-Inositol |
180 |
12.6 |
0.07 |
|
Niacinamide |
122 |
2.02 |
0.0166 |
|
Pyridoxine hydrochloride |
206 |
2 |
0.0097 |
|
Riboflavin |
376 |
0.219 |
0.0006 |
|
Thiamine hydrochloride |
337 |
2.17 |
0.0064 |
|
Vitamin B12 |
1355 |
0.68 |
0.0005 |
注意事项
1. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
2. 本产品仅供科研使用，禁止用于人体。
HB220324
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[1] Zheng Y, Ke L, Lu Y, et al. Enhanced Healing and Antimicrobial Efficacy of Chitosan-g-Polyacrylamide in a Rat Model of Gingival Ulcers. Front Chem. 2020;8:273. Published 2020 Apr 24. doi:10.3389/fchem.2020.00273(IF:3.693)