重组人CEACAM-3蛋白 Recombinant Human CEACAM-3 Protein,hFc Tag

重组人CEACAM-3蛋白 Recombinant Human CEACAM-3 Protein,hFc Tag

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FAQ

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已发表文献

 

性能参数

分子别名(Synonyms)

CGM1;CD66d;CEACAM3

表达区间及表达系统(Source)

Human CEACAM-3 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Lys35-Gly155.[Accession | P40198-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.85 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Human CEACAM-7, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名(Synonyms)

CGM1;CD66d;CEACAM3

表达区间及表达系统(Source)

Human CEACAM-3 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Lys35-Gly155.[Accession | P40198-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.85 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Human CEACAM-7, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.