转录缓冲液 10×Transcription Buffer 2 GMP-grade
产品说明书
FAQ
COA
已发表文献
This product is optimized transcription buffer and efficient for in vitro synthesis of RNA. This product is mainly composed of buffer salts, reducing agents and magnesium ions required for enzyme proteins.
This product is produced in accordance with GMP regulations and provided in liquid form.
Contents
|
Contents No. |
Name |
Catalog No./Specification |
||||
|
10670ES03 |
10670ES10 |
10670ES25 |
10670ES60 |
10670ES76 |
||
|
10670 |
10× Transcription Buffer 2 GMP-grade |
1 mL |
10 mL |
25 mL |
100 mL |
500 mL |
Shipping and Storage
This product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Notes
1. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.
HB230406
This product is optimized transcription buffer and efficient for in vitro synthesis of RNA. This product is mainly composed of buffer salts, reducing agents and magnesium ions required for enzyme proteins.
This product is produced in accordance with GMP regulations and provided in liquid form.
Contents
|
Contents No. |
Name |
Catalog No./Specification |
||||
|
10670ES03 |
10670ES10 |
10670ES25 |
10670ES60 |
10670ES76 |
||
|
10670 |
10× Transcription Buffer 2 GMP-grade |
1 mL |
10 mL |
25 mL |
100 mL |
500 mL |
Shipping and Storage
This product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Notes
1. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.
HB230406
Q: 看到咱家的转录buffer，Mg2+浓度达到460mM，比N*、T*和其他国产厂家的都高。我们看到有关文献报导，Mg2+浓度对转录副产物dsRNA量有着显著影响，我想了解咱家的转录buffer Mg2+浓度做的这么高，一定是有比较全面的研究数据，是否能够分享？
A: 我们转录Buffer是10X的浓度，在IVT体系的终浓度是46mM，是会高于一些其他品牌。之前我们有做过Mg2+单因素研究的一些数据（具体不太方便公开），确定了该使用浓度。我们另外提供不含Mg2+的buffer（10669ES），方便客户自行调配Mg2+在转录体系中的终浓度，从而调节IVT产量和副产物之间的平衡。
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