产品简介

革兰氏染色法是细菌学中广泛使用的一种鉴别染色法（属复染法），包括初染，媒染，脱色，复染等几步，主要用于区分革兰氏阳性菌和革兰氏阴性菌。

染色原理是：革兰氏阳性菌由于其细胞壁较厚，不含类脂，肽聚糖网层次较多且交联致密，经结晶紫初染和碘液媒染后能将结晶紫与碘的复合物牢固留在壁内，不容易被脱色液脱掉而仍呈紫色；而革兰氏阴性菌因其细胞壁较薄、外膜层类脂含量高、肽聚糖层薄且交联度差，在遇脱色剂后，以类脂为主的外膜迅速溶解，薄而松散的肽聚糖不能阻挡结晶紫与碘复合物的溶出，因此通过乙醇脱色后呈无色，再经沙黄等红色染料复染，就使革兰氏阴性菌呈红色。

产品信息

组分信息

储存条件

室温保存，有效期1年。

使用说明

1. 样品准备：按照常规方法准备涂片；

2. 初染：加入结晶紫染液染色1 min，水洗；

3. 媒染：加入碘液染色1 min，水洗；

4. 脱色：加脱色液，摇动玻片，根据涂片厚度，脱色约20-60s，水洗，吸去水分；

5. 复染：加入沙黄染液复染1 min，水洗；

6. 镜检：滤纸吸干或空气中干燥，镜检。

7. 结果判定：革兰氏阳性菌呈紫色，革兰氏阴性菌呈红色。

注意事项

1. 碘液变透明，则不能使用；
2. 水洗时动作需轻柔，沿载玻片对角线方向用洗瓶冲洗，以免把菌体冲掉；
3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
4. 本产品仅用于科研用途。

Ver.CN20240201

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