苏木素伊红(H&E)染色试剂盒 常规染色法|Hematoxylin and Eosin Staining Kit

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苏木素伊红(H&E)染色试剂盒 常规染色法|Hematoxylin and Eosin Staining Kit

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苏木素（Hematoxylin）是一种从洋苏木提取的天然染料，以Mayer’s，Gill’s和Harris等多种配方形式普遍用于细胞核，线粒体，粘蛋白，弹性纤维，肌肉，胶原，髓鞘和磷脂的染色，检测原理是带正电荷的碱性染料苏木素与带负电荷的酸性物质结合使其被染成蓝色。

伊红（Eosin），又称曙红，一种红色荧光染料，在水中解离成带负电荷的阴离子，能够与蛋白质氨基中带正电荷的阳离子结合从而使细胞质、红细胞、胶原、肌纤维、结缔组织、嗜伊红颗粒等染成不同程度的红色或者粉红色。作为一种酸性染料，常用于苏木素-伊红染色法（H&E Staining），用作苏木素的复染剂。

苏木素-伊红（H&E）的结合染色使得细胞质呈红色，细胞核显蓝紫色，红细胞呈桔红色，其它成分呈深浅不同的红色，可用于免疫组化中组织切片，血涂片，骨髓切片的染色，也能用于细胞涂片的染色，是细胞生物学、组织学及病理学等学科必不可少的最常规染色方法之一。

产品组分

组分编号

组分名称

规格

60524-A

苏木素染色液

50 mL

60524-B

伊红染色液

50 mL

60524-C

增色液

100 mL

运输和保存方法

室温运输；室温保存，有效期至少1年。

注意事项

1）为了您的安全和健康，请穿实验服并戴一次性手套操作。
2）本产品仅作科研用途！

使用方法

一、样本前处理

1）石蜡切片

①脱蜡：用无毒环保脱蜡剂或二甲苯脱蜡，10-15 min/次，共2缸2次；

②梯度入水：95%、70%、30%乙醇各2 min，温水2 min，如果脱蜡不干净，需再次温水2 min。此时玻片上除样本部分略有水分外，玻片其余部分均应无水珠。

2）冰冻切片

①回温：按以下方法将预先制作好的并保存在-20℃的冰冻切片取出回温（选取其中一种即可）：

A、室温放置回温5-10 min。

B、37℃孵育箱中进行回温。

C、在37℃水浴箱中放置一个小盒子，再将从-20℃取出的冰冻切片放到小盒当中，目的是让冰冻切片回温至37℃。

②水合：将回温好的切片，水中浸泡30-60 s左右。

【注】：冰冻切片最好用防脱载玻片，切好的片子如不及时染色可放-20℃保存，染色前最好不要用乙醇等固定，否则易造成掉片。

3）血涂片及骨髓涂片

①推片：取全血3 µL左右置载玻片上，将推玻片与载玻片保持30°角，置于血滴正前方，稍往后移与血滴接触，血滴沿推片下缘散开，再匀速沿载玻片平面平稳向前滑动，至血液铺完血膜为止。

②涂片空气中自然干燥，95%乙醇固定2-3 min，水洗约30-60 s。
 

二、 样本染色

1）组织润湿：染色前用蒸馏水润湿组织1-2 min，确保蒸馏水覆盖整个组织，使水分均匀分布；

2）胞核染色：苏木素染色液染色5 min，水洗3-5 s。

3）胞浆染色：可选用以下方法中其中一种进行染色：

①伊红染色液染色10-30 s，用增色液冲洗1-2次，滤纸吸干或自然晾干；

【注】：此法可立即封片镜检。

②伊红染色10-30 s，在水中蘸1-2次（10 s左右），滤纸吸干或自然晾干；

【注】：此法需无水乙醇脱水二次后再封片镜检。

③伊红染色2 min，用蒸馏水洗1-2 min，滤纸吸干或自然晾干；

【注】：此法需无水乙醇脱水二次后再封片镜检。

三、 镜检结果

胞浆呈红色，胞浆呈蓝紫色，红细胞呈桔红色，其它成分呈深浅不同红色。

【注】：若染色较浅，可延长染色时间或者重复染色一次；若染色较深，可延长水冲时间或者再重复梯度脱水一次。

相关产品

产品名称

货号

规格

Hematoxylin stain solution 苏木素染色液

60502JP50/60/76

50ml/100 ml/500 ml

Eosin Y,Disodium Salt (Water Soluble) 伊红Y二钠盐（水溶）

60510JP25/60

25g  /100 g

Eosin Y,Free Acid (Spirit Soluble) 伊红Y（醇溶）

60521JP60

100g

Eosin Stain Solution (Water Soluble) 水溶性伊红染色液

60522JP10/60

10ml/100 ml

Hematoxylin and Eosin Staining Kit 苏木素伊红（H&E）染色试剂盒

60524JP60

2×100 ml

      HB190108

 

苏木素伊红(H&E)染色试剂盒 常规染色法|Hematoxylin and Eosin Staining Kit

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[20] Wang Q, Tao Y, Xie H, Liu C, Liu P. MicroRNA‑101 inhibits renal tubular epithelial‑to‑mesenchymal transition by targeting TGF‑β1 type I receptor. Int J Mol Med. 2021;47(6):119. doi:10.3892/ijmm.2021.4952(IF:4.101)
[21] Shi H, Chi H, Luo Z, et al. Self-Healable, Fast Responsive Poly(ω-Pentadecalactone) Thermogelling System for Effective Liver Cancer Therapy. Front Chem. 2019;7:683. Published 2019 Oct 18. doi:10.3389/fchem.2019.00683(IF:3.782)
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[24] Zeng J, Kang WN, Jin L, Anjum AA, Li GQ. Vacuolar ATPase subunit F is critical for larval survival in Henosepilachna vigintioctopunctata. Insect Mol Biol. 2022;31(2):177-189. doi:10.1111/imb.12749(IF:3.585)
[25] Sun W, Liu J, Zhao R, et al. Knockdown of IFNAR2 reduces the inflammatory response in mouse model of type 1 diabetes. Biochem Biophys Res Commun. 2022;619:9-14. doi:10.1016/j.bbrc.2022.06.028(IF:3.575)
[26] Zhang QL, Wang H, Zhu QH, et al. Genome-Wide Identification and Transcriptomic Analysis of MicroRNAs Across Various Amphioxus Organs Using Deep Sequencing. Front Genet. 2019;10:877. Published 2019 Sep 26. doi:10.3389/fgene.2019.00877(IF:3.517)
[27] Da-Wa ZX, Jun M, Chao-Zheng L, et al. Exosomes Derived from M2 Macrophages Exert a Therapeutic Effect via Inhibition of the PI3K/AKT/mTOR Pathway in Rats with Knee Osteoarthritic. Biomed Res Int. 2021;2021:7218067. Published 2021 Dec 10. doi:10.1155/2021/7218067(IF:3.411)
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苏木素（Hematoxylin）是一种从洋苏木提取的天然染料，以Mayer’s，Gill’s和Harris等多种配方形式普遍用于细胞核，线粒体，粘蛋白，弹性纤维，肌肉，胶原，髓鞘和磷脂的染色，检测原理是带正电荷的碱性染料苏木素与带负电荷的酸性物质结合使其被染成蓝色。

伊红（Eosin），又称曙红，一种红色荧光染料，在水中解离成带负电荷的阴离子，能够与蛋白质氨基中带正电荷的阳离子结合从而使细胞质、红细胞、胶原、肌纤维、结缔组织、嗜伊红颗粒等染成不同程度的红色或者粉红色。作为一种酸性染料，常用于苏木素-伊红染色法（H&E Staining），用作苏木素的复染剂。

苏木素-伊红（H&E）的结合染色使得细胞质呈红色，细胞核显蓝紫色，红细胞呈桔红色，其它成分呈深浅不同的红色，可用于免疫组化中组织切片，血涂片，骨髓切片的染色，也能用于细胞涂片的染色，是细胞生物学、组织学及病理学等学科必不可少的最常规染色方法之一。

产品组分

组分编号

组分名称

规格

60524-A

苏木素染色液

50 mL

60524-B

伊红染色液

50 mL

60524-C

增色液

100 mL

运输和保存方法

室温运输；室温保存，有效期至少1年。

注意事项

1）为了您的安全和健康，请穿实验服并戴一次性手套操作。
2）本产品仅作科研用途！

使用方法

一、样本前处理

1）石蜡切片

①脱蜡：用无毒环保脱蜡剂或二甲苯脱蜡，10-15 min/次，共2缸2次；

②梯度入水：95%、70%、30%乙醇各2 min，温水2 min，如果脱蜡不干净，需再次温水2 min。此时玻片上除样本部分略有水分外，玻片其余部分均应无水珠。

2）冰冻切片

①回温：按以下方法将预先制作好的并保存在-20℃的冰冻切片取出回温（选取其中一种即可）：

A、室温放置回温5-10 min。

B、37℃孵育箱中进行回温。

C、在37℃水浴箱中放置一个小盒子，再将从-20℃取出的冰冻切片放到小盒当中，目的是让冰冻切片回温至37℃。

②水合：将回温好的切片，水中浸泡30-60 s左右。

【注】：冰冻切片最好用防脱载玻片，切好的片子如不及时染色可放-20℃保存，染色前最好不要用乙醇等固定，否则易造成掉片。

3）血涂片及骨髓涂片

①推片：取全血3 µL左右置载玻片上，将推玻片与载玻片保持30°角，置于血滴正前方，稍往后移与血滴接触，血滴沿推片下缘散开，再匀速沿载玻片平面平稳向前滑动，至血液铺完血膜为止。

②涂片空气中自然干燥，95%乙醇固定2-3 min，水洗约30-60 s。
 

二、 样本染色

1）组织润湿：染色前用蒸馏水润湿组织1-2 min，确保蒸馏水覆盖整个组织，使水分均匀分布；

2）胞核染色：苏木素染色液染色5 min，水洗3-5 s。

3）胞浆染色：可选用以下方法中其中一种进行染色：

①伊红染色液染色10-30 s，用增色液冲洗1-2次，滤纸吸干或自然晾干；

【注】：此法可立即封片镜检。

②伊红染色10-30 s，在水中蘸1-2次（10 s左右），滤纸吸干或自然晾干；

【注】：此法需无水乙醇脱水二次后再封片镜检。

③伊红染色2 min，用蒸馏水洗1-2 min，滤纸吸干或自然晾干；

【注】：此法需无水乙醇脱水二次后再封片镜检。

三、 镜检结果

胞浆呈红色，胞浆呈蓝紫色，红细胞呈桔红色，其它成分呈深浅不同红色。

【注】：若染色较浅，可延长染色时间或者重复染色一次；若染色较深，可延长水冲时间或者再重复梯度脱水一次。

相关产品

产品名称

货号

规格

Hematoxylin stain solution 苏木素染色液

60502JP50/60/76

50ml/100 ml/500 ml

Eosin Y,Disodium Salt (Water Soluble) 伊红Y二钠盐（水溶）

60510JP25/60

25g  /100 g

Eosin Y,Free Acid (Spirit Soluble) 伊红Y（醇溶）

60521JP60

100g

Eosin Stain Solution (Water Soluble) 水溶性伊红染色液

60522JP10/60

10ml/100 ml

Hematoxylin and Eosin Staining Kit 苏木素伊红（H&E）染色试剂盒

60524JP60

2×100 ml

      HB190108

 

苏木素伊红(H&E)染色试剂盒 常规染色法|Hematoxylin and Eosin Staining Kit

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