脱脂奶粉Skim Milk 细菌微生物培养基,WB/ELISA封闭剂|=

脱脂奶粉Skim Milk 细菌微生物培养基,WB/ELISA封闭剂|=

产品说明书

FAQ

COA

已发表文献

产品描述

脱脂奶粉(Skim Milk)是将鲜牛奶脱去脂肪再干燥而成,常用于微生物培养基的制备,以及用作免疫实验如Western Blot,ELISA的封闭剂。普通脱脂奶粉由于嗜热微生物的存在常引起有偏差的培养结果,因此不适用于细菌培养基。然而本品经特殊喷雾干燥法所得,不含任何嗜热微生物,达到细菌培养级。

脱脂奶粉可单独或者作为一种复杂细菌培养基的添加成分。基于凝固和酪蛋白水解的原理,脱脂奶粉可用于分化鉴定食品和乳制品微生物。常与pH,石蕊和溴甲酚紫混合使用来分析产酸情况,也常与亚甲蓝和刃天青混合使用建立氧化还原指示系统。也可以用作微生物菌种的保藏。

本品用于封闭的常用浓度为5%(v/v)。

产品使用

首先往干粉内加入少量蒸馏水(ddH2O),搅拌成均匀糊团,然后继续加入蒸馏水该干粉将其稀释成10%(w/v)的混合液,此时的溶液等同于新鲜牛奶,需要对其进行高压灭菌121℃,5min。灭菌时需注意温度切勿过高,否则会发生焦糖化现象。

产品性质

外观(Appearance)

乳白或白色粉末

水分(moisture)

<5%

脂肪含量(Fat content)

≤1.5%

蛋白质(Protein)

≥34%

溶解性(Solubility)

溶于水

运输与保存方法

室温运输。室温密封干燥保存。

注意事项

1)为了您的安全和健康,请穿实验服并戴一次性手套操作。
2)本产品仅作科研用途!

 

脱脂奶粉Skim Milk 细菌微生物培养基,WB/ELISA封闭剂|=

暂无内容

[1] Hu LP, Zhou KX, Huo YM, et al. Single-cell RNA sequencing reveals that targeting HSP90 suppresses PDAC progression by restraining mitochondrial bioenergetics. Oncogenesis. 2021;10(3):22. Published 2021 Mar 3. doi:10.1038/s41389-021-00311-4(IF:7.485)
[2] Wang Y, Zhao M, Li W, et al. BMSC-Derived Small Extracellular Vesicles Induce Cartilage Reconstruction of Temporomandibular Joint Osteoarthritis via Autotaxin-YAP Signaling Axis. Front Cell Dev Biol. 2021;9:656153. Published 2021 Apr 1. doi:10.3389/fcell.2021.656153(IF:6.684)
[3] Yang B, Li Y, Zhang R, et al. MOB1A regulates glucose deprivation-induced autophagy via IL6-STAT3 pathway in gallbladder carcinoma. Am J Cancer Res. 2020;10(11):3896-3910. Published 2020 Nov 1. (IF:5.177)
[4] Ding Y, Xia S, Fang H, Niu B, Chen Q. Loureirin B attenuates insulin resistance in HepG2 cells by regulating gluconeogenesis signaling pathway. Eur J Pharmacol. 2021;910:174481. doi:10.1016/j.ejphar.2021.174481(IF:4.432)
[5] Zhou Y, Zhao WJ, Quan W, et al. Dynamic changes of activated AHR in microglia and astrocytes in the substantia nigra-striatum system in an MPTP-induced Parkinson's disease mouse model. Brain Res Bull. 2021;176:174-183. doi:10.1016/j.brainresbull.2021.08.013(IF:4.079)
[6] Qiao CM, Sun MF, Jia XB, et al. Sodium Butyrate Exacerbates Parkinson's Disease by Aggravating Neuroinflammation and Colonic Inflammation in MPTP-Induced Mice Model. Neurochem Res. 2020;45(9):2128-2142. doi:10.1007/s11064-020-03074-3(IF:3.996)
[7] Qiao CM, Sun MF, Jia XB, et al. Sodium butyrate causes α-synuclein degradation by an Atg5-dependent and PI3K/Akt/mTOR-related autophagy pathway. Exp Cell Res. 2020;387(1):111772. doi:10.1016/j.yexcr.2019.111772(IF:3.905)
[8] Shi Y, Qiao CM, Zhou Y, et al. Protective effects of prucalopride in MPTP-induced Parkinson's disease mice: Neurochemistry, motor function and gut barrier. Biochem Biophys Res Commun. 2021;556:16-22. doi:10.1016/j.bbrc.2021.03.109(IF:3.575)
[9] Pan K, Huang X, Jia X. SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu. Oncol Lett. 2021;21(6):485. doi:10.3892/ol.2021.12746(IF:2.967)
[10] Wang H, Chen X, Kong N, et al. TRIM21 inhibits porcine epidemic diarrhea virus proliferation by proteasomal degradation of the nucleocapsid protein. Arch Virol. 2021;166(7):1903-1911. doi:10.1007/s00705-021-05080-4(IF:2.574)

产品描述

脱脂奶粉(Skim Milk)是将鲜牛奶脱去脂肪再干燥而成,常用于微生物培养基的制备,以及用作免疫实验如Western Blot,ELISA的封闭剂。普通脱脂奶粉由于嗜热微生物的存在常引起有偏差的培养结果,因此不适用于细菌培养基。然而本品经特殊喷雾干燥法所得,不含任何嗜热微生物,达到细菌培养级。

脱脂奶粉可单独或者作为一种复杂细菌培养基的添加成分。基于凝固和酪蛋白水解的原理,脱脂奶粉可用于分化鉴定食品和乳制品微生物。常与pH,石蕊和溴甲酚紫混合使用来分析产酸情况,也常与亚甲蓝和刃天青混合使用建立氧化还原指示系统。也可以用作微生物菌种的保藏。

本品用于封闭的常用浓度为5%(v/v)。

产品使用

首先往干粉内加入少量蒸馏水(ddH2O),搅拌成均匀糊团,然后继续加入蒸馏水该干粉将其稀释成10%(w/v)的混合液,此时的溶液等同于新鲜牛奶,需要对其进行高压灭菌121℃,5min。灭菌时需注意温度切勿过高,否则会发生焦糖化现象。

产品性质

外观(Appearance)

乳白或白色粉末

水分(moisture)

<5%

脂肪含量(Fat content)

≤1.5%

蛋白质(Protein)

≥34%

溶解性(Solubility)

溶于水

运输与保存方法

室温运输。室温密封干燥保存。

注意事项

1)为了您的安全和健康,请穿实验服并戴一次性手套操作。
2)本产品仅作科研用途!

 

脱脂奶粉Skim Milk 细菌微生物培养基,WB/ELISA封闭剂|=

暂无内容

[1] Hu LP, Zhou KX, Huo YM, et al. Single-cell RNA sequencing reveals that targeting HSP90 suppresses PDAC progression by restraining mitochondrial bioenergetics. Oncogenesis. 2021;10(3):22. Published 2021 Mar 3. doi:10.1038/s41389-021-00311-4(IF:7.485)
[2] Wang Y, Zhao M, Li W, et al. BMSC-Derived Small Extracellular Vesicles Induce Cartilage Reconstruction of Temporomandibular Joint Osteoarthritis via Autotaxin-YAP Signaling Axis. Front Cell Dev Biol. 2021;9:656153. Published 2021 Apr 1. doi:10.3389/fcell.2021.656153(IF:6.684)
[3] Yang B, Li Y, Zhang R, et al. MOB1A regulates glucose deprivation-induced autophagy via IL6-STAT3 pathway in gallbladder carcinoma. Am J Cancer Res. 2020;10(11):3896-3910. Published 2020 Nov 1. (IF:5.177)
[4] Ding Y, Xia S, Fang H, Niu B, Chen Q. Loureirin B attenuates insulin resistance in HepG2 cells by regulating gluconeogenesis signaling pathway. Eur J Pharmacol. 2021;910:174481. doi:10.1016/j.ejphar.2021.174481(IF:4.432)
[5] Zhou Y, Zhao WJ, Quan W, et al. Dynamic changes of activated AHR in microglia and astrocytes in the substantia nigra-striatum system in an MPTP-induced Parkinson's disease mouse model. Brain Res Bull. 2021;176:174-183. doi:10.1016/j.brainresbull.2021.08.013(IF:4.079)
[6] Qiao CM, Sun MF, Jia XB, et al. Sodium Butyrate Exacerbates Parkinson's Disease by Aggravating Neuroinflammation and Colonic Inflammation in MPTP-Induced Mice Model. Neurochem Res. 2020;45(9):2128-2142. doi:10.1007/s11064-020-03074-3(IF:3.996)
[7] Qiao CM, Sun MF, Jia XB, et al. Sodium butyrate causes α-synuclein degradation by an Atg5-dependent and PI3K/Akt/mTOR-related autophagy pathway. Exp Cell Res. 2020;387(1):111772. doi:10.1016/j.yexcr.2019.111772(IF:3.905)
[8] Shi Y, Qiao CM, Zhou Y, et al. Protective effects of prucalopride in MPTP-induced Parkinson's disease mice: Neurochemistry, motor function and gut barrier. Biochem Biophys Res Commun. 2021;556:16-22. doi:10.1016/j.bbrc.2021.03.109(IF:3.575)
[9] Pan K, Huang X, Jia X. SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu. Oncol Lett. 2021;21(6):485. doi:10.3892/ol.2021.12746(IF:2.967)
[10] Wang H, Chen X, Kong N, et al. TRIM21 inhibits porcine epidemic diarrhea virus proliferation by proteasomal degradation of the nucleocapsid protein. Arch Virol. 2021;166(7):1903-1911. doi:10.1007/s00705-021-05080-4(IF:2.574)