antibodyresearch 113004 说明书

HyMAXHybridoma Fusion & Cloning Supplement 20x

货号: 113004                  

规格:  100mL





货号: 113004

用法:加入5mL HyMax™20x至95mL您目前用于培养细胞的工作介质,使其占5%。不要使用超过10%的HyMax™。继续用这种培养基培养细胞,直到细胞生长好几代。一旦细胞生长良好,通过逐步将百分比逐步降低到1%,0.5%和0%,可以将培养物从HyMax隔离
储存:建议在4°C至8°C的冰箱中储存,直至6个月内使用。它可以制成等分试样,并在-20°C – 80°C下冷冻保存,长期在12个月内使用。避免反复冻融,这可能会导致活动减少或降水。



图-1用于产生IgG产生B细胞杂交瘤的克隆因子补充物的分析。脾细胞与SP2 / 0细胞融合,培养14天,然后分析来自培养物的上清液。通过抗Fc(γ)ELISA进行分析。IgG阳性克隆被鉴定为产生超过25ng / mL IgG的那些。



  • 无血清生长添加剂
  • 低蛋白含量
  • 成分*已知
  • zui高克隆效率
  • 优良的单克隆抗体生产
  • 无需喂养细胞







Hybridoma Fusion and Cloning Supplement; (HFCS)


10 ml (50x)

Roche Applied Science

Roche Applied Science品牌的 11363735001 已停产

品名 货号 规格 品牌
HyMAX™Hybridoma Fusion & Cloning Supplement 20x 113004 100mL antibodyresearch
HyMAX™Hybridoma Fusion & Cloning Supplement 20x 113004 25ml antibodyresearch


HyMAX™ Hybridoma Fusion and Cloning Supplement, 20X
Catalog No.: 113004
Alternate Names: Hybridoma supplement, Hybridoma cloning factor, HCF, hybridoma cloning supplement, HCS, hybridoma cloning fusion supplement, HCFS, hybridoma growth supplement, HGS, hybridoma fusion and cloning booster, hybridoma fusion and cloning supplement, HFCS, Cell Culture Hybridoma Media Supplements
Description: HyMax™ Hybridoma Fusion and Growth Supplement is a proprietary blend of conditioned media containing various cytokines, growth factors & hormones. It is formulated to replace feeder cells during new hybridoma development. It can also be used for fast recovery of frozen cells from liquid nitrogen.
Feeder cells are routinely used during plating of cells after fusion. Typically feeder cells provide many unknown growth factors to the newly developing clones. These includes many cytokines which are involved in isotype switching and maintenance of IgG secretion by B-cells. From our many years of experience with hybridoma development we have formulated this conditioned media that support better growth & higher frequency of IgG producing clone development. It is tested for hybridoma colony forming efficiency and maintenance of IgG secretion.
Usage: Add 5mL of HyMax™ 20x to 95mL of your working media currently used for culturing cells to make 5%. Do not use HyMax™ at more than 10%. Continue with culturing cells with this media until cells have grown well for few generations. Once cells have grown well, culture may be weaned off of HyMax by gradually reducing the percentage in steps to 1%, 0.5% and 0%.
Format: Provided as a sterile filtered, tissue culture tested, 20x concentrate ready to use solution. Never frozen before shipping to customer. Each 100mL bottle is enough to make 2L of working media, enough for doing a complete hybridoma development project.
Storage: It is recommended to store at 4°C – 8°C in refrigerator until use within 6 months. It can be made into aliquots and frozen stored at -20°C – 80°C for long term to use within a 12 month period. Avoid repeated freezing and thawing, which may cause activity loss and or show precipitation.
Stability: 6 months when stored at 4°C – 8°C in refrigerator or 12 months when stored frozen. Once thawed use within 30 days or aliquot and store as needed. Do not freeze & thaw repeatedly.
Shipping: It is prepared fresh (within 30 days) and shipped in liquid form on blue ice packs.

Third party independent comparisons of HyMax to other products:


Figure-1 Analysis of cloning factor supplements for production of IgG-producing B cell hybridomas. Splenocytes were fused with SP2/0 cells, cultured for 14 days before analysis of supernatant from the cultures. Analysis was performed via anti-Fc (gamma) ELISA. IgG positive clones were identified as those producing more than 25ng/mL IgG.