Human IL-15 Protein 重组人白细胞介素-15(人白介素-15)

Human IL-15 Protein 重组人白细胞介素-15(人白介素-15)

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已发表文献

Human Interleukin-15 (IL-15) is a widely expressed 14 kDa cytokine that is plays an important role in many immunological diseases. It shares approximately 97% and 73% sequence identity with simian and murine IL-15, respectively. Both human and simian IL-15 are active on murine cells. IL-15 is secreted by mononuclear phagocytes (and some other cells), especially macrophages following infection by virus. IL-15 and IL-2 have similar biologic properties in vitro, consistent with their shared receptor (R) signaling components (IL-2/15Rβγc). A role for IL-15 during autoimmune processes such as rheumatoid arthritis and malignancies such as adult T-cell leukemia suggest that dysregulation of IL-15 may result in deleterious effects for the host. 

 

Product Properties

Synonyms

Interleukin-15, IL-15, MGC9721

Accession

P40933

GeneID

3600

Source

E.coli-derived human IL-15 protein, Asn49Ser162.

Molecular Weight

Approximately 12.9 kDa.

AA Sequence

NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Biological Activity

The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1.Avoid repeated freeze-thaw cycles.

2.For your safety and health, please wear lab coats and disposable gloves for operation.

3.For research use only!

HB221018

Human IL-15 Protein 重组人白细胞介素-15(人白介素-15)

暂无内容

Human IL-15 Protein 重组人白细胞介素-15(人白介素-15)

暂无内容

Human Interleukin-15 (IL-15) is a widely expressed 14 kDa cytokine that is plays an important role in many immunological diseases. It shares approximately 97% and 73% sequence identity with simian and murine IL-15, respectively. Both human and simian IL-15 are active on murine cells. IL-15 is secreted by mononuclear phagocytes (and some other cells), especially macrophages following infection by virus. IL-15 and IL-2 have similar biologic properties in vitro, consistent with their shared receptor (R) signaling components (IL-2/15Rβγc). A role for IL-15 during autoimmune processes such as rheumatoid arthritis and malignancies such as adult T-cell leukemia suggest that dysregulation of IL-15 may result in deleterious effects for the host. 

 

Product Properties

Synonyms

Interleukin-15, IL-15, MGC9721

Accession

P40933

GeneID

3600

Source

E.coli-derived human IL-15 protein, Asn49Ser162.

Molecular Weight

Approximately 12.9 kDa.

AA Sequence

NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Biological Activity

The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1.Avoid repeated freeze-thaw cycles.

2.For your safety and health, please wear lab coats and disposable gloves for operation.

3.For research use only!

HB221018

Human IL-15 Protein 重组人白细胞介素-15(人白介素-15)

暂无内容

Human IL-15 Protein 重组人白细胞介素-15(人白介素-15)

暂无内容